AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0344250


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0344250 MtrunA17_CPg0492781 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0344250 MtrunA17_Chr4g0015791 98.333 60 1 0 46 105 7 66 1.14e-38 124
Msa0344250 MtrunA17_Chr8g0355601 96.610 59 2 0 46 104 7 65 1.73e-37 120
Msa0344250 MtrunA17_Chr7g0224991 95.455 44 2 0 62 105 6 49 7.95e-26 90.9
Msa0344250 MtrunA17_CPg0492791 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0344250 MtrunA17_Chr4g0015781 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0344250 MtrunA17_Chr4g0028951 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0344250 MtrunA17_Chr8g0355611 93.750 32 2 0 15 46 1 32 2.06e-15 64.3
Msa0344250 MtrunA17_Chr4g0016881 96.774 31 1 0 15 45 1 31 5.83e-15 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0344250 ATCG00640.1 75.000 60 15 0 46 105 7 66 2.13e-29 100
Msa0344250 ATCG00630.1 84.375 32 5 0 15 46 1 32 8.57e-13 58.2

Find 13 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACGAATAACTGTTATTT+TGG 0.125276 3_1:-2736695 Msa0344250:CDS
AGAGTGTTAATAAGGAATCT+AGG 0.329597 3_1:-2736651 Msa0344250:CDS
AATCGTATGTTTGCAACAAA+AGG 0.396841 3_1:+2736555 None:intergenic
CACCAGTGGCAAGTACTCTA+TGG 0.426592 3_1:-2737321 Msa0344250:CDS
GAGTGTTAATAAGGAATCTA+GGG 0.452009 3_1:-2736650 Msa0344250:CDS
CTATGGTTCGCGGTTCTAGC+AGG 0.487956 3_1:-2737304 Msa0344250:CDS
ACACAATACACCTAGTCGAT+TGG 0.524208 3_1:-2736596 Msa0344250:CDS
GGCAAGTACTCTATGGTTCG+CGG 0.527936 3_1:-2737314 Msa0344250:CDS
ATCGTATGTTTGCAACAAAA+GGG 0.538893 3_1:+2736556 None:intergenic
TTTCTCAATTCCAATCGACT+AGG 0.574270 3_1:+2736586 None:intergenic
ATATCTCTCTGTGGCACCAG+TGG 0.585814 3_1:-2737335 Msa0344250:CDS
AACCATAGAGTACTTGCCAC+TGG 0.586013 3_1:+2737319 None:intergenic
AGTGTTAATAAGGAATCTAG+GGG 0.667965 3_1:-2736649 Msa0344250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACTAGAATCAAAAAAA+GGG + chr3_1:2736696-2736715 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - chr3_1:2736819-2736838 Msa0344250:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + chr3_1:2736913-2736932 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG - chr3_1:2737069-2737088 Msa0344250:intron 15.0%
!! AATAACTAGAATCAAAAAAA+GGG + chr3_1:2736696-2736715 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG - chr3_1:2736819-2736838 Msa0344250:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG + chr3_1:2736913-2736932 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG - chr3_1:2737069-2737088 Msa0344250:intron 15.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG + chr3_1:2736544-2736563 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG - chr3_1:2736543-2736562 Msa0344250:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + chr3_1:2736695-2736714 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + chr3_1:2736697-2736716 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - chr3_1:2736695-2736714 Msa0344250:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + chr3_1:2736750-2736769 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - chr3_1:2736822-2736841 Msa0344250:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - chr3_1:2736828-2736847 Msa0344250:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + chr3_1:2736944-2736963 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG - chr3_1:2737233-2737252 Msa0344250:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - chr3_1:2737269-2737288 Msa0344250:intron 20.0%
!!! TTCGTTTTTTTCTTGTAATT+TGG + chr3_1:2736544-2736563 None:intergenic 20.0%
!! AAATTACAAGAAAAAAACGA+AGG - chr3_1:2736543-2736562 Msa0344250:CDS 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG + chr3_1:2736695-2736714 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG + chr3_1:2736697-2736716 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG - chr3_1:2736695-2736714 Msa0344250:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG + chr3_1:2736750-2736769 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG - chr3_1:2736822-2736841 Msa0344250:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG - chr3_1:2736828-2736847 Msa0344250:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG + chr3_1:2736944-2736963 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG - chr3_1:2737233-2737252 Msa0344250:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG - chr3_1:2737269-2737288 Msa0344250:intron 20.0%
! AAAAAAAAAACGAACGAAGA+GGG + chr3_1:2736767-2736786 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG + chr3_1:2736768-2736787 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - chr3_1:2736948-2736967 Msa0344250:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - chr3_1:2736949-2736968 Msa0344250:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG - chr3_1:2737187-2737206 Msa0344250:intron 25.0%
! AAAAAAAAAACGAACGAAGA+GGG + chr3_1:2736767-2736786 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG + chr3_1:2736768-2736787 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG - chr3_1:2736948-2736967 Msa0344250:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG - chr3_1:2736949-2736968 Msa0344250:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG - chr3_1:2737187-2737206 Msa0344250:intron 25.0%
TTACAAGAAAAAAACGAAGG+AGG - chr3_1:2736546-2736565 Msa0344250:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - chr3_1:2736584-2736603 Msa0344250:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG - chr3_1:2736656-2736675 Msa0344250:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - chr3_1:2736700-2736719 Msa0344250:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG + chr3_1:2736854-2736873 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - chr3_1:2736928-2736947 Msa0344250:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG - chr3_1:2736952-2736971 Msa0344250:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG - chr3_1:2737190-2737209 Msa0344250:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + chr3_1:2737223-2737242 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - chr3_1:2737277-2737296 Msa0344250:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - chr3_1:2737278-2737297 Msa0344250:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - chr3_1:2737279-2737298 Msa0344250:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + chr3_1:2737375-2737394 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + chr3_1:2737376-2737395 None:intergenic 30.0%
TTACAAGAAAAAAACGAAGG+AGG - chr3_1:2736546-2736565 Msa0344250:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG - chr3_1:2736584-2736603 Msa0344250:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG - chr3_1:2736656-2736675 Msa0344250:CDS 30.0%
! TTTGATTCTAGTTATTGGCA+CGG - chr3_1:2736700-2736719 Msa0344250:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG + chr3_1:2736854-2736873 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG - chr3_1:2736928-2736947 Msa0344250:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG - chr3_1:2736952-2736971 Msa0344250:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG - chr3_1:2737190-2737209 Msa0344250:intron 30.0%
TATTCGTATATCTTTACCCT+TGG + chr3_1:2737223-2737242 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG - chr3_1:2737277-2737296 Msa0344250:intron 30.0%
GAGTGTTAATAAGGAATCTA+GGG - chr3_1:2737278-2737297 Msa0344250:intron 30.0%
AGTGTTAATAAGGAATCTAG+GGG - chr3_1:2737279-2737298 Msa0344250:intron 30.0%
ATCGTATGTTTGCAACAAAA+GGG + chr3_1:2737375-2737394 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG + chr3_1:2737376-2737395 None:intergenic 30.0%
! TGAAATTAGGAGTTTTTCCC+AGG - chr3_1:2736857-2736876 Msa0344250:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + chr3_1:2736877-2736896 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + chr3_1:2736878-2736897 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG - chr3_1:2736953-2736972 Msa0344250:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + chr3_1:2737148-2737167 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG - chr3_1:2737197-2737216 Msa0344250:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG + chr3_1:2737345-2737364 None:intergenic 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG - chr3_1:2736857-2736876 Msa0344250:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG + chr3_1:2736877-2736896 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG + chr3_1:2736878-2736897 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG - chr3_1:2736953-2736972 Msa0344250:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG + chr3_1:2737148-2737167 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG - chr3_1:2737197-2737216 Msa0344250:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG + chr3_1:2737345-2737364 None:intergenic 35.0%
GATTTGGCCTCAGTGAAATT+AGG - chr3_1:2736844-2736863 Msa0344250:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - chr3_1:2736866-2736885 Msa0344250:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - chr3_1:2737332-2737351 Msa0344250:CDS 40.0%
GATTTGGCCTCAGTGAAATT+AGG - chr3_1:2736844-2736863 Msa0344250:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG - chr3_1:2736866-2736885 Msa0344250:intron 40.0%
ACACAATACACCTAGTCGAT+TGG - chr3_1:2737332-2737351 Msa0344250:CDS 40.0%
! AACCATAGAGTACTTGCCAC+TGG + chr3_1:2736612-2736631 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG + chr3_1:2736678-2736697 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG - chr3_1:2736705-2736724 Msa0344250:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG - chr3_1:2736706-2736725 Msa0344250:intron 45.0%
! AACCATAGAGTACTTGCCAC+TGG + chr3_1:2736612-2736631 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG + chr3_1:2736678-2736697 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG - chr3_1:2736705-2736724 Msa0344250:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG - chr3_1:2736706-2736725 Msa0344250:intron 45.0%
ATATCTCTCTGTGGCACCAG+TGG - chr3_1:2736593-2736612 Msa0344250:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - chr3_1:2736607-2736626 Msa0344250:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - chr3_1:2736614-2736633 Msa0344250:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG + chr3_1:2736677-2736696 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG - chr3_1:2736707-2736726 Msa0344250:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - chr3_1:2737204-2737223 Msa0344250:intron 50.0%
ATATCTCTCTGTGGCACCAG+TGG - chr3_1:2736593-2736612 Msa0344250:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG - chr3_1:2736607-2736626 Msa0344250:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG - chr3_1:2736614-2736633 Msa0344250:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG + chr3_1:2736677-2736696 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG - chr3_1:2736707-2736726 Msa0344250:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG - chr3_1:2737204-2737223 Msa0344250:intron 50.0%
! CTATGGTTCGCGGTTCTAGC+AGG - chr3_1:2736624-2736643 Msa0344250:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG + chr3_1:2737206-2737225 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG - chr3_1:2737203-2737222 Msa0344250:intron 55.0%
! CTATGGTTCGCGGTTCTAGC+AGG - chr3_1:2736624-2736643 Msa0344250:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG + chr3_1:2737206-2737225 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG - chr3_1:2737203-2737222 Msa0344250:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 2736534 2737416 2736534 ID=Msa0344250;Name=Msa0344250
chr3_1 mRNA 2736534 2737416 2736534 ID=Msa0344250-mRNA-1;Parent=Msa0344250;Name=Msa0344250-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|105
chr3_1 exon 2737284 2737416 2737284 ID=Msa0344250-mRNA-1:exon:889;Parent=Msa0344250-mRNA-1
chr3_1 exon 2736534 2736718 2736534 ID=Msa0344250-mRNA-1:exon:888;Parent=Msa0344250-mRNA-1
chr3_1 CDS 2737284 2737416 2737284 ID=Msa0344250-mRNA-1:cds;Parent=Msa0344250-mRNA-1
chr3_1 CDS 2736534 2736718 2736534 ID=Msa0344250-mRNA-1:cds;Parent=Msa0344250-mRNA-1
Gene Sequence

>Msa0344250

ATGTATTCCAAATTACAAGAAAAAAACGAAGGAGGATTTGAAATGCGAGATCTAAAAACATATCTCTCTGTGGCACCAGTGGCAAGTACTCTATGGTTCGCGGTTCTAGCAGGTCTCTTGATAGAAATCAATCATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACGTCGAGATAAAGAAATAG

Protein sequence

>Msa0344250

MYSKLQEKNEGGFEMRDLKTYLSVAPVASTLWFAVLAGLLIEINHIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHVEIKK*