AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0740720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0740720 MtrunA17_Chr5g0441381 95.833 240 9 1 1 239 1 240 3.18e-171 471
Msa0740720 MtrunA17_Chr7g0246301 40.940 149 78 5 9 155 8 148 6.24e-26 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0740720 AT1G08500.1 52.814 231 96 4 13 239 7 228 8.55e-78 234

Find 58 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCGACATGAAATGAAAT+AGG 0.222292 5_1:+59819864 Msa0740720:five_prime_UTR
TCCCAAGCTGAATTCTTTCT+TGG 0.237475 5_1:-59820242 None:intergenic
GACTCATCGTCACCGGAAAT+TGG 0.237543 5_1:-59821703 None:intergenic
AAAGGAACTGCCACTGTTAC+TGG 0.264232 5_1:-59821529 None:intergenic
AAATTTCACAACATGTTATA+TGG 0.271985 5_1:+59821850 Msa0740720:CDS
TTCCTTAGGATGGTTGAAAT+TGG 0.295594 5_1:-59821749 None:intergenic
CAACTGTTAATTATCAGAAA+TGG 0.300214 5_1:+59820215 Msa0740720:CDS
ATTATTATAAAATAAATAAA+AGG 0.310077 5_1:-59821923 None:intergenic
TCTCCGGCTAGTCCAATTTC+CGG 0.340374 5_1:+59821691 Msa0740720:CDS
TTTCACTCACCCAAGAATAA+TGG 0.363506 5_1:+59820054 Msa0740720:exon
AGCCGGAGACGGAGAATCTT+CGG 0.389032 5_1:-59821677 None:intergenic
TCATCGTCACTGTCTTCCTT+AGG 0.390911 5_1:-59821763 None:intergenic
TCATGTTTCTTAGGTTCTGT+TGG 0.394187 5_1:+59820133 Msa0740720:CDS
TACACAGTGGGAGATTCTTT+AGG 0.400880 5_1:+59820169 Msa0740720:CDS
TTGGATTGTGTCCTTGTCTT+GGG 0.401290 5_1:-59821470 None:intergenic
CTTTAACATCGAAACAGAAC+TGG 0.404508 5_1:-59821821 None:intergenic
ATTGGATTGTGTCCTTGTCT+TGG 0.414041 5_1:-59821471 None:intergenic
AACTGTTAATTATCAGAAAT+GGG 0.420262 5_1:+59820216 Msa0740720:CDS
GGTGAGTGAAATGTGAATAA+TGG 0.437886 5_1:-59820042 None:intergenic
TTATACAACAATAACATAGA+TGG 0.440206 5_1:-59822010 None:intergenic
TTGGAAGGAATATTTGTGTC+TGG 0.465359 5_1:-59821730 None:intergenic
AACCGACATGAAATGAAATA+GGG 0.467134 5_1:+59819865 Msa0740720:five_prime_UTR
GTTGCCCCTTCTTTCAGTAA+AGG 0.467797 5_1:-59821547 None:intergenic
GCAGTTCCTTTACTGAAAGA+AGG 0.475413 5_1:+59821541 Msa0740720:CDS
AAATCACATGTAACCATAGC+TGG 0.482015 5_1:+59819938 Msa0740720:five_prime_UTR
TGACAAAGATAAAGATAAAG+AGG 0.482717 5_1:+59821795 Msa0740720:CDS
GCCAAGAAAGAATTCAGCTT+GGG 0.483597 5_1:+59820241 Msa0740720:CDS
GATTCATATGCTGCCAGCTA+TGG 0.501233 5_1:-59819951 None:intergenic
CAGTGGGAGATTCTTTAGGC+TGG 0.508955 5_1:+59820173 Msa0740720:CDS
ACGGATCAACTGATGACCAT+TGG 0.511597 5_1:-59821489 None:intergenic
CGTCACTGTCTTCCTTAGGA+TGG 0.511800 5_1:-59821759 None:intergenic
TTAGGATGGTTGAAATTGGA+AGG 0.515170 5_1:-59821745 None:intergenic
CATTTCTGATAATTAACAGT+TGG 0.517247 5_1:-59820214 None:intergenic
CAGTTCCTTTACTGAAAGAA+GGG 0.524215 5_1:+59821542 Msa0740720:CDS
TTCAAAATAAATGTGACTCA+TGG 0.525741 5_1:+59821622 Msa0740720:CDS
TTCCAATTTCAACCATCCTA+AGG 0.526093 5_1:+59821747 Msa0740720:CDS
CTCCCTATTTCATTTCATGT+CGG 0.528285 5_1:-59819867 None:intergenic
ATCCGAAGATTCTCCGTCTC+CGG 0.529783 5_1:+59821675 Msa0740720:CDS
TATATGTTTGAACAAGAGAG+TGG 0.543824 5_1:-59821411 None:intergenic
ATAATCACATTGTTTGTATG+TGG 0.560805 5_1:-59821440 None:intergenic
TGCCAAGAAAGAATTCAGCT+TGG 0.561777 5_1:+59820240 Msa0740720:CDS
GTTGACGTGAGATGTGAGCT+AGG 0.566796 5_1:+59821982 Msa0740720:three_prime_UTR
TCACCGGAAATTGGACTAGC+CGG 0.571076 5_1:-59821694 None:intergenic
TGGCGCAGACTCATCGTCAC+CGG 0.575216 5_1:-59821710 None:intergenic
TGAATAATGGCAACTAGCAG+AGG 0.583195 5_1:-59820029 None:intergenic
TACTGGATGTATATCAGTGT+TGG 0.593017 5_1:-59821512 None:intergenic
GGCTCAGTGGATGAGAATGT+AGG 0.619488 5_1:-59819973 None:intergenic
AGTTCCTTTACTGAAAGAAG+GGG 0.634152 5_1:+59821543 Msa0740720:CDS
TTGACGTGAGATGTGAGCTA+GGG 0.640215 5_1:+59821983 Msa0740720:three_prime_UTR
GAAATTGGACTAGCCGGAGA+CGG 0.643836 5_1:-59821688 None:intergenic
TTATGATGATGCCCAAGACA+AGG 0.650355 5_1:+59821459 Msa0740720:CDS
AGTAATTCAACAGAAAGACA+AGG 0.655740 5_1:+59819902 Msa0740720:five_prime_UTR
ATAAATGTGACTCATGGACA+AGG 0.658209 5_1:+59821628 Msa0740720:CDS
GGATGTATATCAGTGTTGGA+CGG 0.669469 5_1:-59821508 None:intergenic
GGAGACGGAGAATCTTCGGA+TGG 0.673058 5_1:-59821673 None:intergenic
TGATATACATCCAGTAACAG+TGG 0.677787 5_1:+59821519 Msa0740720:CDS
AAAATGTCCCTAATAAAACA+AGG 0.680872 5_1:-59821876 None:intergenic
AAGACAAGGACACAATCCAA+TGG 0.682396 5_1:+59821473 Msa0740720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTATAAAATAAATAAA+AGG - chr5_1:59821926-59821945 None:intergenic 0.0%
!! ATTATTATAAAATAAATAAA+AGG - chr5_1:59821926-59821945 None:intergenic 0.0%
!! ATAAAAAATTATGCTTAAAT+TGG + chr5_1:59820855-59820874 Msa0740720:intron 10.0%
!! ATAAAAAATTATGCTTAAAT+TGG + chr5_1:59820855-59820874 Msa0740720:intron 10.0%
!! ACAAATTAAAGAAATGAAAA+CGG - chr5_1:59821169-59821188 None:intergenic 15.0%
!!! TTTCATTTCTTTAATTTGTT+TGG + chr5_1:59821170-59821189 Msa0740720:intron 15.0%
!! ACAAATTAAAGAAATGAAAA+CGG - chr5_1:59821169-59821188 None:intergenic 15.0%
!!! TTTCATTTCTTTAATTTGTT+TGG + chr5_1:59821170-59821189 Msa0740720:intron 15.0%
!!! TTCTATTTTCCATTATTCTT+GGG - chr5_1:59820066-59820085 None:intergenic 20.0%
!! AACTGTTAATTATCAGAAAT+GGG + chr5_1:59820216-59820235 Msa0740720:CDS 20.0%
!! AAAAATAAAATCACTTACTG+AGG - chr5_1:59820273-59820292 None:intergenic 20.0%
!! GAAAAAAAATCAAAGACTAT+AGG - chr5_1:59820303-59820322 None:intergenic 20.0%
!!! TGATTTTTTTTCATGTTTAG+AGG + chr5_1:59820311-59820330 Msa0740720:intron 20.0%
!!! TTTTTTACAAAAGAGACAAA+CGG - chr5_1:59821021-59821040 None:intergenic 20.0%
!! AAGAAAGGAATATTACAAAA+AGG - chr5_1:59821107-59821126 None:intergenic 20.0%
!!! TTTGTAATATTCCTTTCTTT+TGG + chr5_1:59821108-59821127 Msa0740720:intron 20.0%
!! CAAATTAAAGAAATGAAAAC+GGG - chr5_1:59821168-59821187 None:intergenic 20.0%
!!! TCTTTAATTTGTTTGGTTTT+AGG + chr5_1:59821177-59821196 Msa0740720:intron 20.0%
!! AAATTTCACAACATGTTATA+TGG + chr5_1:59821850-59821869 Msa0740720:CDS 20.0%
!! TTATACAACAATAACATAGA+TGG - chr5_1:59822013-59822032 None:intergenic 20.0%
!!! TTCTATTTTCCATTATTCTT+GGG - chr5_1:59820066-59820085 None:intergenic 20.0%
!! AACTGTTAATTATCAGAAAT+GGG + chr5_1:59820216-59820235 Msa0740720:CDS 20.0%
!! AAAAATAAAATCACTTACTG+AGG - chr5_1:59820273-59820292 None:intergenic 20.0%
!! GAAAAAAAATCAAAGACTAT+AGG - chr5_1:59820303-59820322 None:intergenic 20.0%
!!! TGATTTTTTTTCATGTTTAG+AGG + chr5_1:59820311-59820330 Msa0740720:intron 20.0%
!!! TTTTTTACAAAAGAGACAAA+CGG - chr5_1:59821021-59821040 None:intergenic 20.0%
!! AAGAAAGGAATATTACAAAA+AGG - chr5_1:59821107-59821126 None:intergenic 20.0%
!!! TTTGTAATATTCCTTTCTTT+TGG + chr5_1:59821108-59821127 Msa0740720:intron 20.0%
!! CAAATTAAAGAAATGAAAAC+GGG - chr5_1:59821168-59821187 None:intergenic 20.0%
!!! TCTTTAATTTGTTTGGTTTT+AGG + chr5_1:59821177-59821196 Msa0740720:intron 20.0%
!! AAATTTCACAACATGTTATA+TGG + chr5_1:59821850-59821869 Msa0740720:CDS 20.0%
!! TTATACAACAATAACATAGA+TGG - chr5_1:59822013-59822032 None:intergenic 20.0%
!! CTTCTATTTTCCATTATTCT+TGG - chr5_1:59820067-59820086 None:intergenic 25.0%
!! CTTTTTCTATCATGTTTCTT+AGG + chr5_1:59820124-59820143 Msa0740720:CDS 25.0%
! CATTTCTGATAATTAACAGT+TGG - chr5_1:59820217-59820236 None:intergenic 25.0%
! CAACTGTTAATTATCAGAAA+TGG + chr5_1:59820215-59820234 Msa0740720:CDS 25.0%
! TTGCATTGAATTACTTCTAA+TGG + chr5_1:59820400-59820419 Msa0740720:intron 25.0%
!!! GTGTGTTTCAATTTTTTACA+TGG + chr5_1:59820476-59820495 Msa0740720:intron 25.0%
! GTTTGTGATTGATTATTTGA+TGG + chr5_1:59820576-59820595 Msa0740720:intron 25.0%
!!! AGGAGCATAAAATTAGATTT+TGG + chr5_1:59820697-59820716 Msa0740720:intron 25.0%
! AAATAAAAACACAACCACTT+AGG - chr5_1:59820778-59820797 None:intergenic 25.0%
!!! ATGTACTTATATGCTGTTTT+TGG + chr5_1:59820808-59820827 Msa0740720:intron 25.0%
!!! TGTACTTATATGCTGTTTTT+GGG + chr5_1:59820809-59820828 Msa0740720:intron 25.0%
!! TGTTAATCTGTTTTGCTAAA+TGG - chr5_1:59820982-59821001 None:intergenic 25.0%
!! GTTTGTCTCTTTTGTAAAAA+AGG + chr5_1:59821020-59821039 Msa0740720:intron 25.0%
! GAATATTTGTACCAAAAGAA+AGG - chr5_1:59821122-59821141 None:intergenic 25.0%
!!! TTGGTTTTAGGATTAAAGTT+TGG + chr5_1:59821189-59821208 Msa0740720:intron 25.0%
! ATAATCACATTGTTTGTATG+TGG - chr5_1:59821443-59821462 None:intergenic 25.0%
!! TTTTTCTCTAGTGATTATGA+TGG + chr5_1:59821574-59821593 Msa0740720:CDS 25.0%
! TTCAAAATAAATGTGACTCA+TGG + chr5_1:59821622-59821641 Msa0740720:CDS 25.0%
! TGACAAAGATAAAGATAAAG+AGG + chr5_1:59821795-59821814 Msa0740720:CDS 25.0%
! AAAATGTCCCTAATAAAACA+AGG - chr5_1:59821879-59821898 None:intergenic 25.0%
!! CTTCTATTTTCCATTATTCT+TGG - chr5_1:59820067-59820086 None:intergenic 25.0%
!! CTTTTTCTATCATGTTTCTT+AGG + chr5_1:59820124-59820143 Msa0740720:CDS 25.0%
! CATTTCTGATAATTAACAGT+TGG - chr5_1:59820217-59820236 None:intergenic 25.0%
! CAACTGTTAATTATCAGAAA+TGG + chr5_1:59820215-59820234 Msa0740720:CDS 25.0%
! TTGCATTGAATTACTTCTAA+TGG + chr5_1:59820400-59820419 Msa0740720:intron 25.0%
!!! GTGTGTTTCAATTTTTTACA+TGG + chr5_1:59820476-59820495 Msa0740720:intron 25.0%
! GTTTGTGATTGATTATTTGA+TGG + chr5_1:59820576-59820595 Msa0740720:intron 25.0%
!!! AGGAGCATAAAATTAGATTT+TGG + chr5_1:59820697-59820716 Msa0740720:intron 25.0%
! AAATAAAAACACAACCACTT+AGG - chr5_1:59820778-59820797 None:intergenic 25.0%
!!! ATGTACTTATATGCTGTTTT+TGG + chr5_1:59820808-59820827 Msa0740720:intron 25.0%
!!! TGTACTTATATGCTGTTTTT+GGG + chr5_1:59820809-59820828 Msa0740720:intron 25.0%
!! TGTTAATCTGTTTTGCTAAA+TGG - chr5_1:59820982-59821001 None:intergenic 25.0%
!! GTTTGTCTCTTTTGTAAAAA+AGG + chr5_1:59821020-59821039 Msa0740720:intron 25.0%
! GAATATTTGTACCAAAAGAA+AGG - chr5_1:59821122-59821141 None:intergenic 25.0%
!!! TTGGTTTTAGGATTAAAGTT+TGG + chr5_1:59821189-59821208 Msa0740720:intron 25.0%
! ATAATCACATTGTTTGTATG+TGG - chr5_1:59821443-59821462 None:intergenic 25.0%
!! TTTTTCTCTAGTGATTATGA+TGG + chr5_1:59821574-59821593 Msa0740720:CDS 25.0%
! TTCAAAATAAATGTGACTCA+TGG + chr5_1:59821622-59821641 Msa0740720:CDS 25.0%
! TGACAAAGATAAAGATAAAG+AGG + chr5_1:59821795-59821814 Msa0740720:CDS 25.0%
! AAAATGTCCCTAATAAAACA+AGG - chr5_1:59821879-59821898 None:intergenic 25.0%
AAACCGACATGAAATGAAAT+AGG + chr5_1:59819864-59819883 Msa0740720:five_prime_UTR 30.0%
AACCGACATGAAATGAAATA+GGG + chr5_1:59819865-59819884 Msa0740720:five_prime_UTR 30.0%
AGTAATTCAACAGAAAGACA+AGG + chr5_1:59819902-59819921 Msa0740720:five_prime_UTR 30.0%
!!! TAAGTACTAGCTTTTATGAC+AGG - chr5_1:59819997-59820016 None:intergenic 30.0%
AGCTTACAAAAATTACACAG+TGG + chr5_1:59820156-59820175 Msa0740720:CDS 30.0%
GCTTACAAAAATTACACAGT+GGG + chr5_1:59820157-59820176 Msa0740720:CDS 30.0%
! TTTTCATGTTTAGAGGTCTT+TGG + chr5_1:59820318-59820337 Msa0740720:intron 30.0%
TAAATTGGAAATCAGATCGA+AGG - chr5_1:59820447-59820466 None:intergenic 30.0%
ACACACAATTGAGTGTAAAT+TGG - chr5_1:59820462-59820481 None:intergenic 30.0%
TCTCATTAGTAGTTTCTGAA+AGG + chr5_1:59820677-59820696 Msa0740720:intron 30.0%
CATATAAGTACATCACAGTT+GGG - chr5_1:59820801-59820820 None:intergenic 30.0%
!! AAATTGGAAGCACTAAGAAA+AGG + chr5_1:59820871-59820890 Msa0740720:intron 30.0%
!! AATTGGAAGCACTAAGAAAA+GGG + chr5_1:59820872-59820891 Msa0740720:intron 30.0%
! GTCTCTTTTGTAAAAAAGGA+AGG + chr5_1:59821024-59821043 Msa0740720:intron 30.0%
! TTCTCTTTTGATACTATGAG+TGG + chr5_1:59821139-59821158 Msa0740720:intron 30.0%
TGTATTCTTAGTGTGTCTTT+AGG + chr5_1:59821248-59821267 Msa0740720:intron 30.0%
CTAGTATTAACTTCAAAGTG+TGG + chr5_1:59821283-59821302 Msa0740720:intron 30.0%
TATATGTTTGAACAAGAGAG+TGG - chr5_1:59821414-59821433 None:intergenic 30.0%
!!! TATGGATGCCTTGTTTTATT+AGG + chr5_1:59821868-59821887 Msa0740720:CDS 30.0%
!!! ATGGATGCCTTGTTTTATTA+GGG + chr5_1:59821869-59821888 Msa0740720:CDS 30.0%
AAACCGACATGAAATGAAAT+AGG + chr5_1:59819864-59819883 Msa0740720:five_prime_UTR 30.0%
AACCGACATGAAATGAAATA+GGG + chr5_1:59819865-59819884 Msa0740720:five_prime_UTR 30.0%
AGTAATTCAACAGAAAGACA+AGG + chr5_1:59819902-59819921 Msa0740720:five_prime_UTR 30.0%
!!! TAAGTACTAGCTTTTATGAC+AGG - chr5_1:59819997-59820016 None:intergenic 30.0%
AGCTTACAAAAATTACACAG+TGG + chr5_1:59820156-59820175 Msa0740720:CDS 30.0%
GCTTACAAAAATTACACAGT+GGG + chr5_1:59820157-59820176 Msa0740720:CDS 30.0%
! TTTTCATGTTTAGAGGTCTT+TGG + chr5_1:59820318-59820337 Msa0740720:intron 30.0%
TAAATTGGAAATCAGATCGA+AGG - chr5_1:59820447-59820466 None:intergenic 30.0%
ACACACAATTGAGTGTAAAT+TGG - chr5_1:59820462-59820481 None:intergenic 30.0%
TCTCATTAGTAGTTTCTGAA+AGG + chr5_1:59820677-59820696 Msa0740720:intron 30.0%
CATATAAGTACATCACAGTT+GGG - chr5_1:59820801-59820820 None:intergenic 30.0%
!! AAATTGGAAGCACTAAGAAA+AGG + chr5_1:59820871-59820890 Msa0740720:intron 30.0%
!! AATTGGAAGCACTAAGAAAA+GGG + chr5_1:59820872-59820891 Msa0740720:intron 30.0%
! GTCTCTTTTGTAAAAAAGGA+AGG + chr5_1:59821024-59821043 Msa0740720:intron 30.0%
! TTCTCTTTTGATACTATGAG+TGG + chr5_1:59821139-59821158 Msa0740720:intron 30.0%
TGTATTCTTAGTGTGTCTTT+AGG + chr5_1:59821248-59821267 Msa0740720:intron 30.0%
CTAGTATTAACTTCAAAGTG+TGG + chr5_1:59821283-59821302 Msa0740720:intron 30.0%
TATATGTTTGAACAAGAGAG+TGG - chr5_1:59821414-59821433 None:intergenic 30.0%
!!! TATGGATGCCTTGTTTTATT+AGG + chr5_1:59821868-59821887 Msa0740720:CDS 30.0%
!!! ATGGATGCCTTGTTTTATTA+GGG + chr5_1:59821869-59821888 Msa0740720:CDS 30.0%
CTCCCTATTTCATTTCATGT+CGG - chr5_1:59819870-59819889 None:intergenic 35.0%
AAATCACATGTAACCATAGC+TGG + chr5_1:59819938-59819957 Msa0740720:five_prime_UTR 35.0%
GGTGAGTGAAATGTGAATAA+TGG - chr5_1:59820045-59820064 None:intergenic 35.0%
TTTCACTCACCCAAGAATAA+TGG + chr5_1:59820054-59820073 Msa0740720:exon 35.0%
TCATGTTTCTTAGGTTCTGT+TGG + chr5_1:59820133-59820152 Msa0740720:CDS 35.0%
AGAGAGAGATCATGTACTAT+AGG - chr5_1:59820369-59820388 None:intergenic 35.0%
TGAGAAAGTAGATCCACTAT+TGG - chr5_1:59820662-59820681 None:intergenic 35.0%
GCATATAAGTACATCACAGT+TGG - chr5_1:59820802-59820821 None:intergenic 35.0%
ACCTATTTGGGGAATAATGT+GGG - chr5_1:59820937-59820956 None:intergenic 35.0%
GAAGAGTGAATACTTGAGTT+GGG - chr5_1:59820960-59820979 None:intergenic 35.0%
TACTGGATGTATATCAGTGT+TGG - chr5_1:59821515-59821534 None:intergenic 35.0%
TGATATACATCCAGTAACAG+TGG + chr5_1:59821519-59821538 Msa0740720:CDS 35.0%
CAGTTCCTTTACTGAAAGAA+GGG + chr5_1:59821542-59821561 Msa0740720:CDS 35.0%
AGTTCCTTTACTGAAAGAAG+GGG + chr5_1:59821543-59821562 Msa0740720:CDS 35.0%
GATGGTGAACAATGCAAAAA+TGG + chr5_1:59821592-59821611 Msa0740720:CDS 35.0%
ATAAATGTGACTCATGGACA+AGG + chr5_1:59821628-59821647 Msa0740720:CDS 35.0%
TTGGAAGGAATATTTGTGTC+TGG - chr5_1:59821733-59821752 None:intergenic 35.0%
TTAGGATGGTTGAAATTGGA+AGG - chr5_1:59821748-59821767 None:intergenic 35.0%
TTCCAATTTCAACCATCCTA+AGG + chr5_1:59821747-59821766 Msa0740720:CDS 35.0%
TTCCTTAGGATGGTTGAAAT+TGG - chr5_1:59821752-59821771 None:intergenic 35.0%
CTTTAACATCGAAACAGAAC+TGG - chr5_1:59821824-59821843 None:intergenic 35.0%
CTCCCTATTTCATTTCATGT+CGG - chr5_1:59819870-59819889 None:intergenic 35.0%
AAATCACATGTAACCATAGC+TGG + chr5_1:59819938-59819957 Msa0740720:five_prime_UTR 35.0%
GGTGAGTGAAATGTGAATAA+TGG - chr5_1:59820045-59820064 None:intergenic 35.0%
TTTCACTCACCCAAGAATAA+TGG + chr5_1:59820054-59820073 Msa0740720:exon 35.0%
TCATGTTTCTTAGGTTCTGT+TGG + chr5_1:59820133-59820152 Msa0740720:CDS 35.0%
AGAGAGAGATCATGTACTAT+AGG - chr5_1:59820369-59820388 None:intergenic 35.0%
TGAGAAAGTAGATCCACTAT+TGG - chr5_1:59820662-59820681 None:intergenic 35.0%
GCATATAAGTACATCACAGT+TGG - chr5_1:59820802-59820821 None:intergenic 35.0%
ACCTATTTGGGGAATAATGT+GGG - chr5_1:59820937-59820956 None:intergenic 35.0%
GAAGAGTGAATACTTGAGTT+GGG - chr5_1:59820960-59820979 None:intergenic 35.0%
TACTGGATGTATATCAGTGT+TGG - chr5_1:59821515-59821534 None:intergenic 35.0%
TGATATACATCCAGTAACAG+TGG + chr5_1:59821519-59821538 Msa0740720:CDS 35.0%
CAGTTCCTTTACTGAAAGAA+GGG + chr5_1:59821542-59821561 Msa0740720:CDS 35.0%
AGTTCCTTTACTGAAAGAAG+GGG + chr5_1:59821543-59821562 Msa0740720:CDS 35.0%
GATGGTGAACAATGCAAAAA+TGG + chr5_1:59821592-59821611 Msa0740720:CDS 35.0%
ATAAATGTGACTCATGGACA+AGG + chr5_1:59821628-59821647 Msa0740720:CDS 35.0%
TTGGAAGGAATATTTGTGTC+TGG - chr5_1:59821733-59821752 None:intergenic 35.0%
TTAGGATGGTTGAAATTGGA+AGG - chr5_1:59821748-59821767 None:intergenic 35.0%
TTCCAATTTCAACCATCCTA+AGG + chr5_1:59821747-59821766 Msa0740720:CDS 35.0%
TTCCTTAGGATGGTTGAAAT+TGG - chr5_1:59821752-59821771 None:intergenic 35.0%
CTTTAACATCGAAACAGAAC+TGG - chr5_1:59821824-59821843 None:intergenic 35.0%
TGAATAATGGCAACTAGCAG+AGG - chr5_1:59820032-59820051 None:intergenic 40.0%
TACACAGTGGGAGATTCTTT+AGG + chr5_1:59820169-59820188 Msa0740720:CDS 40.0%
TGCCAAGAAAGAATTCAGCT+TGG + chr5_1:59820240-59820259 Msa0740720:CDS 40.0%
GCCAAGAAAGAATTCAGCTT+GGG + chr5_1:59820241-59820260 Msa0740720:CDS 40.0%
TCCCAAGCTGAATTCTTTCT+TGG - chr5_1:59820245-59820264 None:intergenic 40.0%
AATGTCAACACCACCAATAG+TGG + chr5_1:59820646-59820665 Msa0740720:intron 40.0%
GAAAGTAGATCCACTATTGG+TGG - chr5_1:59820659-59820678 None:intergenic 40.0%
ACTATAGTGTCACACCTAAG+TGG + chr5_1:59820761-59820780 Msa0740720:intron 40.0%
GGGATGTACTATTCTTGTTC+TGG + chr5_1:59820892-59820911 Msa0740720:intron 40.0%
! ACTATTCTTGTTCTGGACAG+TGG + chr5_1:59820899-59820918 Msa0740720:intron 40.0%
ACCCACATTATTCCCCAAAT+AGG + chr5_1:59820933-59820952 Msa0740720:intron 40.0%
GACCTATTTGGGGAATAATG+TGG - chr5_1:59820938-59820957 None:intergenic 40.0%
!! ACTTGAGTTGGGACCTATTT+GGG - chr5_1:59820949-59820968 None:intergenic 40.0%
!! TACTTGAGTTGGGACCTATT+TGG - chr5_1:59820950-59820969 None:intergenic 40.0%
GGAAGAGTGAATACTTGAGT+TGG - chr5_1:59820961-59820980 None:intergenic 40.0%
! TTATGATGATGCCCAAGACA+AGG + chr5_1:59821459-59821478 Msa0740720:CDS 40.0%
TTGGATTGTGTCCTTGTCTT+GGG - chr5_1:59821473-59821492 None:intergenic 40.0%
ATTGGATTGTGTCCTTGTCT+TGG - chr5_1:59821474-59821493 None:intergenic 40.0%
AAGACAAGGACACAATCCAA+TGG + chr5_1:59821473-59821492 Msa0740720:CDS 40.0%
! GGATGTATATCAGTGTTGGA+CGG - chr5_1:59821511-59821530 None:intergenic 40.0%
GCAGTTCCTTTACTGAAAGA+AGG + chr5_1:59821541-59821560 Msa0740720:CDS 40.0%
!! GATGGCTTTTGAAGACTCTT+CGG - chr5_1:59821658-59821677 None:intergenic 40.0%
TGAATAATGGCAACTAGCAG+AGG - chr5_1:59820032-59820051 None:intergenic 40.0%
TACACAGTGGGAGATTCTTT+AGG + chr5_1:59820169-59820188 Msa0740720:CDS 40.0%
TGCCAAGAAAGAATTCAGCT+TGG + chr5_1:59820240-59820259 Msa0740720:CDS 40.0%
GCCAAGAAAGAATTCAGCTT+GGG + chr5_1:59820241-59820260 Msa0740720:CDS 40.0%
TCCCAAGCTGAATTCTTTCT+TGG - chr5_1:59820245-59820264 None:intergenic 40.0%
AATGTCAACACCACCAATAG+TGG + chr5_1:59820646-59820665 Msa0740720:intron 40.0%
GAAAGTAGATCCACTATTGG+TGG - chr5_1:59820659-59820678 None:intergenic 40.0%
ACTATAGTGTCACACCTAAG+TGG + chr5_1:59820761-59820780 Msa0740720:intron 40.0%
GGGATGTACTATTCTTGTTC+TGG + chr5_1:59820892-59820911 Msa0740720:intron 40.0%
! ACTATTCTTGTTCTGGACAG+TGG + chr5_1:59820899-59820918 Msa0740720:intron 40.0%
ACCCACATTATTCCCCAAAT+AGG + chr5_1:59820933-59820952 Msa0740720:intron 40.0%
GACCTATTTGGGGAATAATG+TGG - chr5_1:59820938-59820957 None:intergenic 40.0%
!! ACTTGAGTTGGGACCTATTT+GGG - chr5_1:59820949-59820968 None:intergenic 40.0%
!! TACTTGAGTTGGGACCTATT+TGG - chr5_1:59820950-59820969 None:intergenic 40.0%
GGAAGAGTGAATACTTGAGT+TGG - chr5_1:59820961-59820980 None:intergenic 40.0%
! TTATGATGATGCCCAAGACA+AGG + chr5_1:59821459-59821478 Msa0740720:CDS 40.0%
TTGGATTGTGTCCTTGTCTT+GGG - chr5_1:59821473-59821492 None:intergenic 40.0%
ATTGGATTGTGTCCTTGTCT+TGG - chr5_1:59821474-59821493 None:intergenic 40.0%
AAGACAAGGACACAATCCAA+TGG + chr5_1:59821473-59821492 Msa0740720:CDS 40.0%
! GGATGTATATCAGTGTTGGA+CGG - chr5_1:59821511-59821530 None:intergenic 40.0%
GCAGTTCCTTTACTGAAAGA+AGG + chr5_1:59821541-59821560 Msa0740720:CDS 40.0%
!! GATGGCTTTTGAAGACTCTT+CGG - chr5_1:59821658-59821677 None:intergenic 40.0%
GATTCATATGCTGCCAGCTA+TGG - chr5_1:59819954-59819973 None:intergenic 45.0%
! AGCTTTTATGACAGGCTCAG+TGG - chr5_1:59819989-59820008 None:intergenic 45.0%
!! CTTGAGTTGGGACCTATTTG+GGG - chr5_1:59820948-59820967 None:intergenic 45.0%
AATGCCAAAGTGACAGTTGC+TGG + chr5_1:59821080-59821099 Msa0740720:intron 45.0%
ATGCCAAAGTGACAGTTGCT+GGG + chr5_1:59821081-59821100 Msa0740720:intron 45.0%
ACGGATCAACTGATGACCAT+TGG - chr5_1:59821492-59821511 None:intergenic 45.0%
AAAGGAACTGCCACTGTTAC+TGG - chr5_1:59821532-59821551 None:intergenic 45.0%
GTTGCCCCTTCTTTCAGTAA+AGG - chr5_1:59821550-59821569 None:intergenic 45.0%
TCATCGTCACTGTCTTCCTT+AGG - chr5_1:59821766-59821785 None:intergenic 45.0%
TTGACGTGAGATGTGAGCTA+GGG + chr5_1:59821983-59822002 Msa0740720:three_prime_UTR 45.0%
GATTCATATGCTGCCAGCTA+TGG - chr5_1:59819954-59819973 None:intergenic 45.0%
! AGCTTTTATGACAGGCTCAG+TGG - chr5_1:59819989-59820008 None:intergenic 45.0%
!! CTTGAGTTGGGACCTATTTG+GGG - chr5_1:59820948-59820967 None:intergenic 45.0%
AATGCCAAAGTGACAGTTGC+TGG + chr5_1:59821080-59821099 Msa0740720:intron 45.0%
ATGCCAAAGTGACAGTTGCT+GGG + chr5_1:59821081-59821100 Msa0740720:intron 45.0%
ACGGATCAACTGATGACCAT+TGG - chr5_1:59821492-59821511 None:intergenic 45.0%
AAAGGAACTGCCACTGTTAC+TGG - chr5_1:59821532-59821551 None:intergenic 45.0%
GTTGCCCCTTCTTTCAGTAA+AGG - chr5_1:59821550-59821569 None:intergenic 45.0%
TCATCGTCACTGTCTTCCTT+AGG - chr5_1:59821766-59821785 None:intergenic 45.0%
TTGACGTGAGATGTGAGCTA+GGG + chr5_1:59821983-59822002 Msa0740720:three_prime_UTR 45.0%
GGCTCAGTGGATGAGAATGT+AGG - chr5_1:59819976-59819995 None:intergenic 50.0%
CAGTGGGAGATTCTTTAGGC+TGG + chr5_1:59820173-59820192 Msa0740720:CDS 50.0%
ATCCGAAGATTCTCCGTCTC+CGG + chr5_1:59821675-59821694 Msa0740720:CDS 50.0%
!! GAAATTGGACTAGCCGGAGA+CGG - chr5_1:59821691-59821710 None:intergenic 50.0%
! TCTCCGGCTAGTCCAATTTC+CGG + chr5_1:59821691-59821710 Msa0740720:CDS 50.0%
!! TCACCGGAAATTGGACTAGC+CGG - chr5_1:59821697-59821716 None:intergenic 50.0%
GACTCATCGTCACCGGAAAT+TGG - chr5_1:59821706-59821725 None:intergenic 50.0%
CGTCACTGTCTTCCTTAGGA+TGG - chr5_1:59821762-59821781 None:intergenic 50.0%
GTTGACGTGAGATGTGAGCT+AGG + chr5_1:59821982-59822001 Msa0740720:three_prime_UTR 50.0%
GGCTCAGTGGATGAGAATGT+AGG - chr5_1:59819976-59819995 None:intergenic 50.0%
CAGTGGGAGATTCTTTAGGC+TGG + chr5_1:59820173-59820192 Msa0740720:CDS 50.0%
ATCCGAAGATTCTCCGTCTC+CGG + chr5_1:59821675-59821694 Msa0740720:CDS 50.0%
!! GAAATTGGACTAGCCGGAGA+CGG - chr5_1:59821691-59821710 None:intergenic 50.0%
! TCTCCGGCTAGTCCAATTTC+CGG + chr5_1:59821691-59821710 Msa0740720:CDS 50.0%
!! TCACCGGAAATTGGACTAGC+CGG - chr5_1:59821697-59821716 None:intergenic 50.0%
GACTCATCGTCACCGGAAAT+TGG - chr5_1:59821706-59821725 None:intergenic 50.0%
CGTCACTGTCTTCCTTAGGA+TGG - chr5_1:59821762-59821781 None:intergenic 50.0%
GTTGACGTGAGATGTGAGCT+AGG + chr5_1:59821982-59822001 Msa0740720:three_prime_UTR 50.0%
! AGGCCCAGCAACTGTCACTT+TGG - chr5_1:59821087-59821106 None:intergenic 55.0%
GGAGACGGAGAATCTTCGGA+TGG - chr5_1:59821676-59821695 None:intergenic 55.0%
AGCCGGAGACGGAGAATCTT+CGG - chr5_1:59821680-59821699 None:intergenic 55.0%
! AGGCCCAGCAACTGTCACTT+TGG - chr5_1:59821087-59821106 None:intergenic 55.0%
GGAGACGGAGAATCTTCGGA+TGG - chr5_1:59821676-59821695 None:intergenic 55.0%
AGCCGGAGACGGAGAATCTT+CGG - chr5_1:59821680-59821699 None:intergenic 55.0%
TGGCGCAGACTCATCGTCAC+CGG - chr5_1:59821713-59821732 None:intergenic 60.0%
TGGCGCAGACTCATCGTCAC+CGG - chr5_1:59821713-59821732 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 59819861 59822085 59819861 ID=Msa0740720;Name=Msa0740720
chr5_1 mRNA 59819861 59822085 59819861 ID=Msa0740720-mRNA-1;Parent=Msa0740720;Name=Msa0740720-mRNA-1;_AED=0.00;_eAED=0.00;_QI=212|1|1|1|1|1|2|176|239
chr5_1 exon 59819861 59820274 59819861 ID=Msa0740720-mRNA-1:exon:17236;Parent=Msa0740720-mRNA-1
chr5_1 exon 59821392 59822085 59821392 ID=Msa0740720-mRNA-1:exon:17237;Parent=Msa0740720-mRNA-1
chr5_1 five_prime_UTR 59819861 59820072 59819861 ID=Msa0740720-mRNA-1:five_prime_utr;Parent=Msa0740720-mRNA-1
chr5_1 CDS 59820073 59820274 59820073 ID=Msa0740720-mRNA-1:cds;Parent=Msa0740720-mRNA-1
chr5_1 CDS 59821392 59821909 59821392 ID=Msa0740720-mRNA-1:cds;Parent=Msa0740720-mRNA-1
chr5_1 three_prime_UTR 59821910 59822085 59821910 ID=Msa0740720-mRNA-1:three_prime_utr;Parent=Msa0740720-mRNA-1
Gene Sequence

>Msa0740720

ATGGAAAATAGAAGAAACTCATCATTGTTTCTATTCTCAACTCTTTCTTGTCTTTTTCTATCATGTTTCTTAGGTTCTGTTGGAGCTTACAAAAATTACACAGTGGGAGATTCTTTAGGCTGGTTTGACAATCTTGAAAAGCCAACTGTTAATTATCAGAAATGGGTTGCCAAGAAAGAATTCAGCTTGGGAGATTTCCTCATTTTCAACACAGACAATAACCACTCTCTTGTTCAAACATATAATTTCACCACATACAAACAATGTGATTATGATGATGCCCAAGACAAGGACACAATCCAATGGTCATCAGTTGATCCGTCCAACACTGATATACATCCAGTAACAGTGGCAGTTCCTTTACTGAAAGAAGGGGCAACATACTTTTTCTCTAGTGATTATGATGGTGAACAATGCAAAAATGGACAACATTTCAAAATAAATGTGACTCATGGACAAGGTTTGCCGAAGAGTCTTCAAAAGCCATCCGAAGATTCTCCGTCTCCGGCTAGTCCAATTTCCGGTGACGATGAGTCTGCGCCAGACACAAATATTCCTTCCAATTTCAACCATCCTAAGGAAGACAGTGACGATGAAAAAACTAGTGACAAAGATAAAGATAAAGAGGAATCCAGTTCTGTTTCGATGTTAAAGTATGCAAAATTTCACAACATGTTATATGGATGCCTTGTTTTATTAGGGACATTTTTCTTTTTTTAG
Protein sequence

>Msa0740720

MENRRNSSLFLFSTLSCLFLSCFLGSVGAYKNYTVGDSLGWFDNLEKPTVNYQKWVAKKEFSLGDFLIFNTDNNHSLVQTYNFTTYKQCDYDDAQDKDTIQWSSVDPSNTDIHPVTVAVPLLKEGATYFFSSDYDGEQCKNGQHFKINVTHGQGLPKSLQKPSEDSPSPASPISGDDESAPDTNIPSNFNHPKEDSDDEKTSDKDKDKEESSSVSMLKYAKFHNMLYGCLVLLGTFFFF*