AlfalfaGEDB | Msa1379590

M. sativa cultivar zhongmu-4 / Msa1379590

KEGG: K08332
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
PFAM: PF00514.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1379590	XP_003594249.1	98.696	460	2	1	1	456	1	460	0.0	912

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1379590	sp\|O22193\|PUB4_ARATH	41.781	438	228	9	35	449	387	820	1.77e-82	274

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1379590	G7IP90	98.696	460	2	1	1	456	1	460	0.0	912

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002680	Msa1379590	0.815206	1.040940e-51	-8.615850e-47
Msa0002710	Msa1379590	0.803769	2.976135e-49	-8.615850e-47
Msa0003050	Msa1379590	0.806273	8.911182e-50	-8.615850e-47
Msa0003300	Msa1379590	0.878834	2.037457e-69	-8.615850e-47
Msa0007990	Msa1379590	0.801813	7.541871e-49	-8.615850e-47
Msa0011930	Msa1379590	0.810930	9.020735e-51	-8.615850e-47
Msa0014570	Msa1379590	0.845001	5.330600e-59	-8.615850e-47
Msa0022550	Msa1379590	0.805997	1.018583e-49	-8.615850e-47
Msa0022750	Msa1379590	-0.849336	3.451818e-60	-8.615850e-47
Msa0029850	Msa1379590	0.846680	1.865453e-59	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1379590	MtrunA17_Chr2g0290151	98.696	460	2	1	1	456	1	460	0.0	912
Msa1379590	MtrunA17_Chr1g0152561	44.444	360	161	2	98	448	9	338	6.42e-92	282
Msa1379590	MtrunA17_Chr3g0134761	48.693	306	141	3	152	453	44	337	2.94e-90	278
Msa1379590	MtrunA17_Chr1g0183781	52.330	279	132	1	175	453	444	721	3.53e-82	268
Msa1379590	MtrunA17_Chr3g0106931	50.530	283	139	1	167	449	549	830	1.74e-76	255
Msa1379590	MtrunA17_Chr7g0261371	50.000	278	138	1	175	452	518	794	8.87e-76	252
Msa1379590	MtrunA17_Chr4g0044411	44.444	279	155	0	171	449	373	651	6.29e-68	228
Msa1379590	MtrunA17_Chr4g0031431	47.547	265	139	0	186	450	368	632	7.39e-68	228
Msa1379590	MtrunA17_Chr1g0199321	47.518	282	148	0	171	452	345	626	1.29e-67	227
Msa1379590	MtrunA17_Chr7g0237931	44.944	267	145	1	178	444	341	605	3.98e-61	209

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1379590	AT4G16490.1	71.882	473	113	8	1	455	1	471	0.0	611
Msa1379590	AT3G01400.1	45.257	369	171	2	81	448	4	342	6.03e-93	285
Msa1379590	AT5G58680.1	43.902	369	175	5	82	448	3	341	1.12e-86	269
Msa1379590	AT2G23140.3	41.781	438	228	9	35	449	198	631	2.06e-85	275
Msa1379590	AT2G23140.5	41.781	438	228	9	35	449	198	631	2.06e-85	275
Msa1379590	AT2G23140.1	41.781	438	228	9	35	449	390	823	1.62e-83	274
Msa1379590	AT2G23140.2	41.781	438	228	9	35	449	387	820	1.80e-83	274
Msa1379590	AT2G23140.4	41.781	438	228	9	35	449	387	820	1.80e-83	274
Msa1379590	AT5G67340.1	49.828	291	144	2	160	449	412	701	1.47e-77	256
Msa1379590	AT5G67340.1	33.702	181	106	5	152	329	536	705	9.51e-11	64.7

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Find 156 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTCAATTGGAGCTTC+AGG	0.285745	tig0020280:+70369	Msa1379590:CDS
AAAATATTAGTACAAGTTTC+TGG	0.277209	tig0020280:-72381	None:intergenic
AAACACAACAAATTGGAACA+AGG	0.643203	tig0020280:-71743	None:intergenic
AAAGAGAGAGCGGTGAGTGC+TGG	0.499031	tig0020280:+70492	Msa1379590:CDS
AAAGCTCAATTGGAGCTTCA+GGG	0.489989	tig0020280:+70370	Msa1379590:CDS
AACACAACAAATTGGAACAA+GGG	0.602875	tig0020280:-71742	None:intergenic
AACACACGCCATGTAACTCC+TGG	0.489743	tig0020280:-71766	None:intergenic
AACAGAAGCGAAACCAACGG+TGG	0.719199	tig0020280:-70399	None:intergenic
AACAGATGAAGCTGAGTAAC+TGG	0.382820	tig0020280:-72157	None:intergenic
AACATCCTTCCAAGAATGTT+TGG	0.368032	tig0020280:-71632	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAATCTCAAATTCGTTACAG+TGG	+	tig0020280:69474-69493	Msa1379590:five_prime_UTR	30.0%
GAAGAGAATAAAAGCTCAAT+TGG	+	tig0020280:70360-70379	Msa1379590:CDS	30.0%
TTCATACCATAGGATTCAAA+AGG	+	tig0020280:70847-70866	Msa1379590:intron	30.0%
AGATATCTTAAATAACCAGG+TGG	-	tig0020280:70909-70928	None:intergenic	30.0%
ATATCTTGACTTTGAGTGTT+TGG	+	tig0020280:70923-70942	Msa1379590:intron	30.0%
TAATAAACCTCGACAAGAAA+AGG	+	tig0020280:71072-71091	Msa1379590:intron	30.0%
AATAAACCTCGACAAGAAAA+GGG	+	tig0020280:71073-71092	Msa1379590:intron	30.0%
AGCTATATTCATGCATCTAA+AGG	-	tig0020280:71703-71722	None:intergenic	30.0%
TTAGATGCATGAATATAGCT+AGG	+	tig0020280:71703-71722	Msa1379590:three_prime_UTR	30.0%
AACACAACAAATTGGAACAA+GGG	-	tig0020280:71745-71764	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0020280	gene	69284	72588	69284	ID=Msa1379590;Name=Msa1379590
tig0020280	mRNA	69284	72588	69284	ID=Msa1379590-mRNA-1;Parent=Msa1379590;Name=Msa1379590-mRNA-1;_AED=0.00;_eAED=0.00;_QI=230\|1\|1\|1\|1\|1\|2\|1054\|456
tig0020280	exon	69284	70821	69284	ID=Msa1379590-mRNA-1:exon:6627;Parent=Msa1379590-mRNA-1
tig0020280	exon	71472	72588	71472	ID=Msa1379590-mRNA-1:exon:6628;Parent=Msa1379590-mRNA-1
tig0020280	five_prime_UTR	69284	69513	69284	ID=Msa1379590-mRNA-1:five_prime_utr;Parent=Msa1379590-mRNA-1
tig0020280	CDS	69514	70821	69514	ID=Msa1379590-mRNA-1:cds;Parent=Msa1379590-mRNA-1
tig0020280	CDS	71472	71534	71472	ID=Msa1379590-mRNA-1:cds;Parent=Msa1379590-mRNA-1
tig0020280	three_prime_UTR	71535	72588	71535	ID=Msa1379590-mRNA-1:three_prime_utr;Parent=Msa1379590-mRNA-1

Gene Sequence

>Msa1379590

ATGGTTTCTCTAGAAGATTCTCGTTCCAATTCAAATCGTTTTCCATTACCAAAAACCTATCAATATCATTCATCTATTTCTTCAAAATCACAGCGAAACATAGGAAGATCCATGCGAACAAGAAGATCCAGTATCTTCGATGAAGATAACAGTAGCTGCACTTTCACCGAAAGATCCACCTGTGTATCGGAAAATCTCACCGATTCTATCGTCGACCTCCGTCTCGGAGAACTCGCTTCCAAGAATCACAAATCCGGAAAATCATCAACTTCCGAACAAGACCTTCTAGATCTTTCTCAAGCTTTTAGTGAATATGCTTATTCAGCTTGTAGTAGTGATATCTCCGGTGAGCTTCAGAGGTTAGCGACGCTGCCGTCACCGGAGTGTGACGGTGGAGGTGATGGTGTGGTTGAAGTTGTTGAACCTGAGCCTTGCATGGGGTTTTTACAAAGAGAGAATTTCTCGACGGAGATTATTGAGAGTATTTCGCCGGAGGATCTTCAACCGACAGTGAAGCTTTGTGTGGATGGTTTACAGTCTTCTTCAGTGGCTGTGAAACGGTCTGCTGCTGCAAAATTGAGGTTGCTTGCGAAGAATCGTGCTGATAATCGTGTTTTGATTGGTGAATCAGGTGCTGTACCTTTGCTTGTTCCTTTGCTTCGGTGTAGTGATCCATGGACACAAGAGCACGCGGTGACGGCGTTGTTGAATCTTTCTCTTCATGAAGATAACAAGAAACTGATATTTAATGCTGGAGCTGTGAAATCGTTGATTTATGTATTGAAAACAGGGACAGAGACTTCTAAGCAGAACGCGGCTTGTGCTCTTTTGAGTTTAGCGTTGGTGGAAGAGAATAAAAGCTCAATTGGAGCTTCAGGGGCGATTCCACCGTTGGTTTCGCTTCTGTTGAATGGTTCCAACAGAGGGAAGAAAGATGCATTAACGACGTTGTACAAGCTTTGTTCTGTTAAGCAGAACAAAGAGAGAGCGGTGAGTGCTGGTGTGGTGAAGCCGTTGGTGGAGCTGGTGGCTGAGCAAGGGAATGGGATGATGGAGAAAGCAATGGTGGTGTTGAATAGCTTAGCTGGAATTGACGAAGGGAAGGAAGCGATTGTGGAAGAAGGTGGAATCGCGGCGCTTGTGGAAGCCATTGAAGATGGGTCTGTGAAAGGAAAGGAATTTGCTGTTTTAACACTTTTGCAGCTTTGTGCTGAAAGTGTTACAAACAGAGGATTGCTTGTTAGAGAAGGTGGGATTCCTCCTCTTGTCGCTCTTTCGCAGAATGGAACTCCTCGAGCTAAGCATAAGGCTGAGACGCTTCTTCGATATTTGCGAGAATCAAGACAAGAAGCATCAACTTCAACTTCGTAG

Protein sequence

>Msa1379590

MVSLEDSRSNSNRFPLPKTYQYHSSISSKSQRNIGRSMRTRRSSIFDEDNSSCTFTERSTCVSENLTDSIVDLRLGELASKNHKSGKSSTSEQDLLDLSQAFSEYAYSACSSDISGELQRLATLPSPECDGGGDGVVEVVEPEPCMGFLQRENFSTEIIESISPEDLQPTVKLCVDGLQSSSVAVKRSAAAKLRLLAKNRADNRVLIGESGAVPLLVPLLRCSDPWTQEHAVTALLNLSLHEDNKKLIFNAGAVKSLIYVLKTGTETSKQNAACALLSLALVEENKSSIGASGAIPPLVSLLLNGSNRGKKDALTTLYKLCSVKQNKERAVSAGVVKPLVELVAEQGNGMMEKAMVVLNSLAGIDEGKEAIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCAESVTNRGLLVREGGIPPLVALSQNGTPRAKHKAETLLRYLRESRQEASTSTS*