Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0238230 | XP_013462764.1 | 93.913 | 115 | 1 | 1 | 1 | 109 | 1 | 115 | 4.08e-70 | 215 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0238230 | A0A072V456 | 93.913 | 115 | 1 | 1 | 1 | 109 | 1 | 115 | 1.95e-70 | 215 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0008220 | Msa0238230 | 0.804729 | 1.878254e-49 | -8.615850e-47 |
| Msa0011930 | Msa0238230 | 0.806726 | 7.150537e-50 | -8.615850e-47 |
| Msa0014570 | Msa0238230 | 0.837250 | 5.807993e-57 | -8.615850e-47 |
| Msa0022750 | Msa0238230 | -0.814961 | 1.180225e-51 | -8.615850e-47 |
| Msa0030280 | Msa0238230 | 0.823286 | 1.503102e-53 | -8.615850e-47 |
| Msa0032320 | Msa0238230 | 0.867567 | 1.244322e-65 | -8.615850e-47 |
| Msa0039250 | Msa0238230 | 0.822188 | 2.707037e-53 | -8.615850e-47 |
| Msa0055070 | Msa0238230 | 0.834628 | 2.685351e-56 | -8.615850e-47 |
| Msa0077450 | Msa0238230 | 0.812259 | 4.638132e-51 | -8.615850e-47 |
| Msa0077880 | Msa0238230 | 0.811771 | 5.924559e-51 | -8.615850e-47 |
| Msa0090390 | Msa0238230 | 0.823584 | 1.280106e-53 | -8.615850e-47 |
| Msa0101020 | Msa0238230 | 0.833978 | 3.909881e-56 | -8.615850e-47 |
| Msa0101880 | Msa0238230 | 0.828983 | 6.635447e-55 | -8.615850e-47 |
| Msa0104700 | Msa0238230 | 0.800885 | 1.168496e-48 | -8.615850e-47 |
| Msa0108390 | Msa0238230 | 0.855426 | 6.359696e-62 | -8.615850e-47 |
| Msa0115830 | Msa0238230 | 0.814993 | 1.160631e-51 | -8.615850e-47 |
| Msa0118000 | Msa0238230 | 0.808696 | 2.728710e-50 | -8.615850e-47 |
| Msa0133720 | Msa0238230 | 0.834068 | 3.710107e-56 | -8.615850e-47 |
| Msa0133820 | Msa0238230 | 0.838190 | 3.331966e-57 | -8.615850e-47 |
| Msa0134000 | Msa0238230 | 0.808425 | 3.117105e-50 | -8.615850e-47 |
| Msa0137870 | Msa0238230 | 0.817674 | 2.917501e-52 | -8.615850e-47 |
| Msa0142460 | Msa0238230 | 0.828226 | 1.011254e-54 | -8.615850e-47 |
| Msa0145070 | Msa0238230 | 0.802338 | 5.882676e-49 | -8.615850e-47 |
| Msa0145110 | Msa0238230 | 0.829610 | 4.675331e-55 | -8.615850e-47 |
| Msa0150990 | Msa0238230 | 0.810022 | 1.417164e-50 | -8.615850e-47 |
| Msa0154440 | Msa0238230 | 0.832572 | 8.756530e-56 | -8.615850e-47 |
| Msa0160670 | Msa0238230 | 0.804400 | 2.199634e-49 | -8.615850e-47 |
| Msa0165300 | Msa0238230 | 0.802704 | 4.945594e-49 | -8.615850e-47 |
| Msa0178510 | Msa0238230 | 0.809763 | 1.611338e-50 | -8.615850e-47 |
| Msa0181000 | Msa0238230 | 0.807399 | 5.152205e-50 | -8.615850e-47 |
| Msa0181230 | Msa0238230 | 0.812903 | 3.354486e-51 | -8.615850e-47 |
| Msa0187030 | Msa0238230 | 0.838559 | 2.675570e-57 | -8.615850e-47 |
| Msa0191890 | Msa0238230 | 0.837625 | 4.653588e-57 | -8.615850e-47 |
| Msa0193540 | Msa0238230 | 0.801510 | 8.703896e-49 | -8.615850e-47 |
| Msa0196340 | Msa0238230 | 0.961290 | 1.568728e-119 | -8.615850e-47 |
| Msa0207350 | Msa0238230 | 0.810927 | 9.034028e-51 | -8.615850e-47 |
| Msa0208810 | Msa0238230 | 0.800977 | 1.118999e-48 | -8.615850e-47 |
| Msa0212750 | Msa0238230 | -0.816253 | 6.084439e-52 | -8.615850e-47 |
| Msa0230370 | Msa0238230 | 0.866610 | 2.514175e-65 | -8.615850e-47 |
| Msa0233380 | Msa0238230 | 0.810196 | 1.300194e-50 | -8.615850e-47 |
| Msa0236070 | Msa0238230 | 0.813330 | 2.703746e-51 | -8.615850e-47 |
| Msa0236700 | Msa0238230 | -0.805864 | 1.086707e-49 | -8.615850e-47 |
| Msa0238230 | Msa0251050 | 0.800621 | 1.322482e-48 | -8.615850e-47 |
| Msa0238230 | Msa0261160 | 0.805994 | 1.020069e-49 | -8.615850e-47 |
| Msa0238230 | Msa0275660 | 0.874072 | 8.986997e-68 | -8.615850e-47 |
| Msa0238230 | Msa0277420 | 0.835917 | 1.268863e-56 | -8.615850e-47 |
| Msa0238230 | Msa0312530 | 0.846287 | 2.387778e-59 | -8.615850e-47 |
| Msa0238230 | Msa0317270 | 0.835379 | 1.736429e-56 | -8.615850e-47 |
| Msa0238230 | Msa0340690 | 0.806631 | 7.491091e-50 | -8.615850e-47 |
| Msa0238230 | Msa0346990 | 0.813881 | 2.045343e-51 | -8.615850e-47 |
| Msa0238230 | Msa0387370 | 0.815911 | 7.252992e-52 | -8.615850e-47 |
| Msa0238230 | Msa0388030 | -0.811136 | 8.140413e-51 | -8.615850e-47 |
| Msa0238230 | Msa0392200 | 0.823613 | 1.260841e-53 | -8.615850e-47 |
| Msa0238230 | Msa0403660 | 0.848265 | 6.842173e-60 | -8.615850e-47 |
| Msa0238230 | Msa0429090 | 0.804624 | 1.975111e-49 | -8.615850e-47 |
| Msa0238230 | Msa0431770 | 0.809773 | 1.603535e-50 | -8.615850e-47 |
| Msa0238230 | Msa0436370 | 0.842268 | 2.868923e-58 | -8.615850e-47 |
| Msa0238230 | Msa0437510 | 0.860774 | 1.633935e-63 | -8.615850e-47 |
| Msa0238230 | Msa0450350 | 0.802144 | 6.449524e-49 | -8.615850e-47 |
| Msa0238230 | Msa0462220 | 0.832053 | 1.177009e-55 | -8.615850e-47 |
| Msa0238230 | Msa0465920 | 0.810495 | 1.120299e-50 | -8.615850e-47 |
| Msa0238230 | Msa0465960 | 0.810596 | 1.065298e-50 | -8.615850e-47 |
| Msa0238230 | Msa0466760 | 0.809441 | 1.889018e-50 | -8.615850e-47 |
| Msa0238230 | Msa0475500 | 0.801089 | 1.061449e-48 | -8.615850e-47 |
| Msa0238230 | Msa0517490 | 0.803468 | 3.437046e-49 | -8.615850e-47 |
| Msa0238230 | Msa0556010 | 0.806230 | 9.097852e-50 | -8.615850e-47 |
| Msa0238230 | Msa0572480 | 0.830565 | 2.733398e-55 | -8.615850e-47 |
| Msa0238230 | Msa0576890 | 0.820983 | 5.141327e-53 | -8.615850e-47 |
| Msa0238230 | Msa0578090 | 0.807517 | 4.863668e-50 | -8.615850e-47 |
| Msa0238230 | Msa0581690 | 0.808927 | 2.435360e-50 | -8.615850e-47 |
| Msa0238230 | Msa0595600 | 0.804821 | 1.796704e-49 | -8.615850e-47 |
| Msa0238230 | Msa0600410 | -0.820969 | 5.178137e-53 | -8.615850e-47 |
| Msa0238230 | Msa0608200 | 0.831352 | 1.752695e-55 | -8.615850e-47 |
| Msa0238230 | Msa0619180 | 0.844288 | 8.293791e-59 | -8.615850e-47 |
| Msa0238230 | Msa0633760 | 0.834432 | 3.008214e-56 | -8.615850e-47 |
| Msa0238230 | Msa0648520 | 0.847995 | 8.122115e-60 | -8.615850e-47 |
| Msa0238230 | Msa0664760 | 0.805054 | 1.606697e-49 | -8.615850e-47 |
| Msa0238230 | Msa0682140 | 0.801216 | 9.996750e-49 | -8.615850e-47 |
| Msa0238230 | Msa0689760 | 0.809290 | 2.036113e-50 | -8.615850e-47 |
| Msa0238230 | Msa0691050 | 0.862671 | 4.295468e-64 | -8.615850e-47 |
| Msa0238230 | Msa0716860 | 0.808101 | 3.654247e-50 | -8.615850e-47 |
| Msa0238230 | Msa0718070 | 0.831883 | 1.296901e-55 | -8.615850e-47 |
| Msa0238230 | Msa0740720 | 0.836263 | 1.036883e-56 | -8.615850e-47 |
| Msa0238230 | Msa0743680 | 0.821081 | 4.879700e-53 | -8.615850e-47 |
| Msa0238230 | Msa0750250 | 0.821903 | 3.151646e-53 | -8.615850e-47 |
| Msa0238230 | Msa0751260 | 0.828353 | 9.422374e-55 | -8.615850e-47 |
| Msa0238230 | Msa0753770 | 0.801000 | 1.106549e-48 | -8.615850e-47 |
| Msa0238230 | Msa0756530 | 0.807225 | 5.609633e-50 | -8.615850e-47 |
| Msa0238230 | Msa0757800 | 0.811266 | 7.629367e-51 | -8.615850e-47 |
| Msa0238230 | Msa0779550 | 0.800759 | 1.239775e-48 | -8.615850e-47 |
| Msa0238230 | Msa0784410 | 0.820972 | 5.171222e-53 | -8.615850e-47 |
| Msa0238230 | Msa0784620 | 0.825793 | 3.860937e-54 | -8.615850e-47 |
| Msa0238230 | Msa0786130 | 0.811063 | 8.445128e-51 | -8.615850e-47 |
| Msa0238230 | Msa0795070 | 0.820744 | 5.835447e-53 | -8.615850e-47 |
| Msa0238230 | Msa0799060 | 0.808758 | 2.645821e-50 | -8.615850e-47 |
| Msa0238230 | Msa0818160 | 0.808813 | 2.575310e-50 | -8.615850e-47 |
| Msa0238230 | Msa0824730 | 0.808804 | 2.586522e-50 | -8.615850e-47 |
| Msa0238230 | Msa0861450 | 0.840337 | 9.242031e-58 | -8.615850e-47 |
| Msa0238230 | Msa0861830 | 0.805610 | 1.228349e-49 | -8.615850e-47 |
| Msa0238230 | Msa0863800 | 0.808866 | 2.508974e-50 | -8.615850e-47 |
| Msa0238230 | Msa0895310 | 0.804357 | 2.245477e-49 | -8.615850e-47 |
| Msa0238230 | Msa0922030 | 0.807179 | 5.734899e-50 | -8.615850e-47 |
| Msa0238230 | Msa0929950 | 0.821632 | 3.641141e-53 | -8.615850e-47 |
| Msa0238230 | Msa0945120 | 0.822213 | 2.671447e-53 | -8.615850e-47 |
| Msa0238230 | Msa0947530 | -0.829621 | 4.645721e-55 | -8.615850e-47 |
| Msa0238230 | Msa1000930 | 0.823682 | 1.214654e-53 | -8.615850e-47 |
| Msa0238230 | Msa1010070 | 0.821880 | 3.190711e-53 | -8.615850e-47 |
| Msa0238230 | Msa1021510 | 0.838416 | 2.914044e-57 | -8.615850e-47 |
| Msa0238230 | Msa1031820 | 0.803383 | 3.577966e-49 | -8.615850e-47 |
| Msa0238230 | Msa1033660 | 0.827014 | 1.976298e-54 | -8.615850e-47 |
| Msa0238230 | Msa1033970 | 0.815350 | 9.668295e-52 | -8.615850e-47 |
| Msa0238230 | Msa1037650 | -0.809828 | 1.560279e-50 | -8.615850e-47 |
| Msa0238230 | Msa1087510 | -0.809673 | 1.684765e-50 | -8.615850e-47 |
| Msa0238230 | Msa1094540 | -0.815171 | 1.059833e-51 | -8.615850e-47 |
| Msa0238230 | Msa1105500 | 0.831721 | 1.421478e-55 | -8.615850e-47 |
| Msa0238230 | Msa1118610 | 0.806302 | 8.786240e-50 | -8.615850e-47 |
| Msa0238230 | Msa1118760 | 0.811924 | 5.488446e-51 | -8.615850e-47 |
| Msa0238230 | Msa1138320 | 0.806626 | 7.507242e-50 | -8.615850e-47 |
| Msa0238230 | Msa1152950 | 0.806874 | 6.653053e-50 | -8.615850e-47 |
| Msa0238230 | Msa1158660 | 0.832830 | 7.554329e-56 | -8.615850e-47 |
| Msa0238230 | Msa1158770 | 0.802041 | 6.773003e-49 | -8.615850e-47 |
| Msa0238230 | Msa1162830 | 0.821499 | 3.909578e-53 | -8.615850e-47 |
| Msa0238230 | Msa1163540 | 0.808056 | 3.735427e-50 | -8.615850e-47 |
| Msa0238230 | Msa1177780 | 0.824650 | 7.195672e-54 | -8.615850e-47 |
| Msa0238230 | Msa1178370 | 0.816186 | 6.297303e-52 | -8.615850e-47 |
| Msa0238230 | Msa1182770 | 0.802544 | 5.334707e-49 | -8.615850e-47 |
| Msa0238230 | Msa1198880 | 0.819696 | 1.014269e-52 | -8.615850e-47 |
| Msa0238230 | Msa1204100 | -0.818258 | 2.153099e-52 | -8.615850e-47 |
| Msa0238230 | Msa1211050 | -0.810570 | 1.079292e-50 | -8.615850e-47 |
| Msa0238230 | Msa1223330 | 0.829070 | 6.321156e-55 | -8.615850e-47 |
| Msa0238230 | Msa1286550 | 0.809540 | 1.798761e-50 | -8.615850e-47 |
| Msa0238230 | Msa1296300 | 0.812102 | 5.018199e-51 | -8.615850e-47 |
| Msa0238230 | Msa1302060 | 0.806401 | 8.374212e-50 | -8.615850e-47 |
| Msa0238230 | Msa1307550 | 0.815265 | 1.009915e-51 | -8.615850e-47 |
| Msa0238230 | Msa1317580 | 0.808435 | 3.101702e-50 | -8.615850e-47 |
| Msa0238230 | Msa1319820 | 0.842038 | 3.299766e-58 | -8.615850e-47 |
| Msa0238230 | Msa1329050 | -0.810566 | 1.081325e-50 | -8.615850e-47 |
| Msa0238230 | Msa1338830 | 0.806972 | 6.345938e-50 | -8.615850e-47 |
| Msa0238230 | Msa1340040 | -0.820449 | 6.820432e-53 | -8.615850e-47 |
| Msa0238230 | Msa1345320 | 0.929351 | 7.868969e-93 | -8.615850e-47 |
| Msa0238230 | Msa1346750 | 0.807239 | 5.570893e-50 | -8.615850e-47 |
| Msa0238230 | Msa1352540 | -0.809503 | 1.832708e-50 | -8.615850e-47 |
| Msa0238230 | Msa1359920 | 0.808639 | 2.805474e-50 | -8.615850e-47 |
| Msa0238230 | Msa1366300 | 0.821915 | 3.132680e-53 | -8.615850e-47 |
| Msa0238230 | Msa1379590 | 0.823160 | 1.608483e-53 | -8.615850e-47 |
| Msa0238230 | Msa1379850 | 0.842665 | 2.251177e-58 | -8.615850e-47 |
| Msa0238230 | Msa1390120 | 0.845970 | 2.912549e-59 | -8.615850e-47 |
| Msa0238230 | Msa1395530 | 0.847216 | 1.330654e-59 | -8.615850e-47 |
| Msa0238230 | Msa1396780 | 0.865299 | 6.529160e-65 | -8.615850e-47 |
| Msa0238230 | Msa1401270 | 0.836239 | 1.051518e-56 | -8.615850e-47 |
| Msa0238230 | Msa1410420 | 0.800171 | 1.633006e-48 | -8.615850e-47 |
| Msa0238230 | Msa1420250 | 0.807481 | 4.950103e-50 | -8.615850e-47 |
| Msa0238230 | Msa1422840 | 0.807097 | 5.968622e-50 | -8.615850e-47 |
| Msa0238230 | Msa1422910 | 0.808716 | 2.701331e-50 | -8.615850e-47 |
| Msa0238230 | Msa1424640 | 0.824647 | 7.205519e-54 | -8.615850e-47 |
| Msa0238230 | Msa1433220 | 0.844612 | 6.786249e-59 | -8.615850e-47 |
| Msa0238230 | Msa1435400 | 0.827994 | 1.150236e-54 | -8.615850e-47 |
| Msa0238230 | Msa1443610 | 0.822410 | 2.404653e-53 | -8.615850e-47 |
| Msa0238230 | Msa1447110 | 0.838702 | 2.457326e-57 | -8.615850e-47 |
| Msa0238230 | Msa1449910 | 0.868396 | 6.736539e-66 | -8.615850e-47 |
| Msa0238230 | Msa1453360 | 0.815007 | 1.152437e-51 | -8.615850e-47 |
| Msa0238230 | Msa1454530 | 0.801993 | 6.927431e-49 | -8.615850e-47 |
| Msa0238230 | Msa1464290 | 0.803727 | 3.036521e-49 | -8.615850e-47 |
| Msa0238230 | Msa1465680 | 0.804386 | 2.215117e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0238230 | MtrunA17_Chr2g0287961 | 93.913 | 115 | 1 | 1 | 1 | 109 | 1 | 115 | 3.75e-74 | 215 |
| Msa0238230 | MtrunA17_Chr4g0074501 | 68.519 | 108 | 26 | 3 | 1 | 100 | 1 | 108 | 8.74e-48 | 148 |
| Msa0238230 | MtrunA17_Chr0c30g0494111 | 64.179 | 67 | 16 | 3 | 42 | 100 | 1 | 67 | 7.92e-22 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0238230 | AT3G06070.1 | 40.625 | 96 | 52 | 3 | 1 | 91 | 1 | 96 | 5.58e-17 | 72.0 |
| Msa0238230 | AT5G19190.1 | 45.263 | 95 | 41 | 4 | 1 | 88 | 1 | 91 | 2.43e-16 | 70.1 |
Find 39 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTTCTTGTACCTCCTTCT+TGG | 0.150935 | 2_2:-11642792 | None:intergenic |
| CTAGAAACTAAATACAATTT+TGG | 0.181446 | 2_2:+11642203 | Msa0238230:five_prime_UTR |
| CTTCATCTTCATCCATTATT+AGG | 0.195790 | 2_2:-11642322 | None:intergenic |
| ATTTAGGTGTATATAATATA+TGG | 0.250639 | 2_2:-11643075 | None:intergenic |
| ATACATGATGACTAATATTT+AGG | 0.266098 | 2_2:-11643091 | None:intergenic |
| TTAATATCATTAACAGAAAA+AGG | 0.277524 | 2_2:-11643197 | None:intergenic |
| TAATTAATTCTTTAGCCTTT+AGG | 0.283220 | 2_2:+11642983 | Msa0238230:three_prime_UTR |
| TTCTATTCTTAGTTATATAA+AGG | 0.312892 | 2_2:+11643032 | Msa0238230:three_prime_UTR |
| GGAAATTGCTCTCCTAATAA+TGG | 0.320698 | 2_2:+11642310 | Msa0238230:CDS |
| TTCTTGGTTGTGTTGCTTGT+GGG | 0.344306 | 2_2:-11642776 | None:intergenic |
| TCAACCTGCAAACTCTCTTC+AGG | 0.344380 | 2_2:+11642358 | Msa0238230:CDS |
| AATTAACATGGAGGTTGCTT+TGG | 0.348717 | 2_2:+11642250 | Msa0238230:exon |
| CATGGAGGTTGCTTTGGAAA+TGG | 0.372075 | 2_2:+11642256 | Msa0238230:exon |
| TGTTTGATAGAATTCTTGTT+AGG | 0.386930 | 2_2:-11642861 | None:intergenic |
| GCCTTTAGGCAATAATTGTA+TGG | 0.401046 | 2_2:+11642997 | Msa0238230:three_prime_UTR |
| AGAAGAGAAAGCAAAGGAAC+AGG | 0.411669 | 2_2:+11642744 | Msa0238230:CDS |
| ATCACACAGCTTTAACTAGA+AGG | 0.419335 | 2_2:-11642909 | None:intergenic |
| CTTCTTGGTTGTGTTGCTTG+TGG | 0.425984 | 2_2:-11642777 | None:intergenic |
| AGAACCTGAAGAGAGTTTGC+AGG | 0.436707 | 2_2:-11642362 | None:intergenic |
| AGTGGAGGTTGCAAAACCTT+TGG | 0.437819 | 2_2:-11643126 | None:intergenic |
| AAATATTAGTCATCATGTAT+AGG | 0.461723 | 2_2:+11643094 | Msa0238230:three_prime_UTR |
| AATTCTATCAAACAGTGAAA+TGG | 0.469397 | 2_2:+11642870 | Msa0238230:exon |
| GCCATACAATTATTGCCTAA+AGG | 0.474343 | 2_2:-11642998 | None:intergenic |
| TATAAAGGTGGATATGTACA+TGG | 0.524484 | 2_2:+11643047 | Msa0238230:three_prime_UTR |
| AAGAAATCAAACTGTGGAGG+AGG | 0.531737 | 2_2:-11642702 | None:intergenic |
| CCAAGAAGGAGGTACAAGAA+AGG | 0.544633 | 2_2:+11642792 | Msa0238230:CDS |
| CACAAGCAACACAACCAAGA+AGG | 0.551661 | 2_2:+11642778 | Msa0238230:CDS |
| TATTCTTAGTTATATAAAGG+TGG | 0.569532 | 2_2:+11643035 | Msa0238230:three_prime_UTR |
| GTATAGGAGGATTCAACCAA+AGG | 0.585124 | 2_2:+11643110 | Msa0238230:three_prime_UTR |
| TATTAGTCATCATGTATAGG+AGG | 0.591401 | 2_2:+11643097 | Msa0238230:three_prime_UTR |
| TTGATTAGAAGAGAAAGCAA+AGG | 0.610534 | 2_2:+11642738 | Msa0238230:CDS |
| TTTGCAGGTTGAGAAGCAAG+TGG | 0.620205 | 2_2:-11642347 | None:intergenic |
| ATAAAGGTGGATATGTACAT+GGG | 0.623401 | 2_2:+11643048 | Msa0238230:three_prime_UTR |
| TAGAAGAAATCAAACTGTGG+AGG | 0.634181 | 2_2:-11642705 | None:intergenic |
| AAGCAACACAACCAAGAAGG+AGG | 0.648331 | 2_2:+11642781 | Msa0238230:CDS |
| TTTATATTGAATTAACATGG+AGG | 0.678774 | 2_2:+11642241 | Msa0238230:exon |
| AATGCTTGTCCACACAGTGG+AGG | 0.692722 | 2_2:-11643141 | None:intergenic |
| CAAAATGCTTGTCCACACAG+TGG | 0.720567 | 2_2:-11643144 | None:intergenic |
| TCATAGAAGAAATCAAACTG+TGG | 0.762193 | 2_2:-11642708 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAAACTAATTTTATTTTGT+AGG | + | chr2_2:11642659-11642678 | Msa0238230:intron | 10.0% |
| !!! | TTAAACTAATTTTATTTTGT+AGG | + | chr2_2:11642659-11642678 | Msa0238230:intron | 10.0% |
| !!! | TCTTTTATATTGAATTAACA+TGG | + | chr2_2:11642238-11642257 | Msa0238230:exon | 15.0% |
| !! | TTCTATTCTTAGTTATATAA+AGG | + | chr2_2:11643032-11643051 | Msa0238230:three_prime_UTR | 15.0% |
| !! | ATTTAGGTGTATATAATATA+TGG | - | chr2_2:11643078-11643097 | None:intergenic | 15.0% |
| !! | TTAATATCATTAACAGAAAA+AGG | - | chr2_2:11643200-11643219 | None:intergenic | 15.0% |
| !!! | TCTTTTATATTGAATTAACA+TGG | + | chr2_2:11642238-11642257 | Msa0238230:exon | 15.0% |
| !! | TTCTATTCTTAGTTATATAA+AGG | + | chr2_2:11643032-11643051 | Msa0238230:three_prime_UTR | 15.0% |
| !! | ATTTAGGTGTATATAATATA+TGG | - | chr2_2:11643078-11643097 | None:intergenic | 15.0% |
| !! | TTAATATCATTAACAGAAAA+AGG | - | chr2_2:11643200-11643219 | None:intergenic | 15.0% |
| !!! | CTAGAAACTAAATACAATTT+TGG | + | chr2_2:11642203-11642222 | Msa0238230:five_prime_UTR | 20.0% |
| !! | TTTATATTGAATTAACATGG+AGG | + | chr2_2:11642241-11642260 | Msa0238230:exon | 20.0% |
| !! | TATATTAGTATGTAACTATG+AGG | - | chr2_2:11642421-11642440 | None:intergenic | 20.0% |
| !! | AAAATGTCATAATTGAAACT+TGG | - | chr2_2:11642476-11642495 | None:intergenic | 20.0% |
| !! | TTAAAGAATTTATTTGTGCA+TGG | + | chr2_2:11642498-11642517 | Msa0238230:intron | 20.0% |
| !!! | ATTTTGCATATAAAGAAGAA+GGG | - | chr2_2:11642631-11642650 | None:intergenic | 20.0% |
| !!! | TATTTTGCATATAAAGAAGA+AGG | - | chr2_2:11642632-11642651 | None:intergenic | 20.0% |
| !!! | TAATTAATTCTTTAGCCTTT+AGG | + | chr2_2:11642983-11643002 | Msa0238230:three_prime_UTR | 20.0% |
| !! | TATTCTTAGTTATATAAAGG+TGG | + | chr2_2:11643035-11643054 | Msa0238230:three_prime_UTR | 20.0% |
| !! | ATACATGATGACTAATATTT+AGG | - | chr2_2:11643094-11643113 | None:intergenic | 20.0% |
| !! | AAATATTAGTCATCATGTAT+AGG | + | chr2_2:11643094-11643113 | Msa0238230:three_prime_UTR | 20.0% |
| !!! | CTAGAAACTAAATACAATTT+TGG | + | chr2_2:11642203-11642222 | Msa0238230:five_prime_UTR | 20.0% |
| !! | TTTATATTGAATTAACATGG+AGG | + | chr2_2:11642241-11642260 | Msa0238230:exon | 20.0% |
| !! | TATATTAGTATGTAACTATG+AGG | - | chr2_2:11642421-11642440 | None:intergenic | 20.0% |
| !! | AAAATGTCATAATTGAAACT+TGG | - | chr2_2:11642476-11642495 | None:intergenic | 20.0% |
| !! | TTAAAGAATTTATTTGTGCA+TGG | + | chr2_2:11642498-11642517 | Msa0238230:intron | 20.0% |
| !!! | ATTTTGCATATAAAGAAGAA+GGG | - | chr2_2:11642631-11642650 | None:intergenic | 20.0% |
| !!! | TATTTTGCATATAAAGAAGA+AGG | - | chr2_2:11642632-11642651 | None:intergenic | 20.0% |
| !!! | TAATTAATTCTTTAGCCTTT+AGG | + | chr2_2:11642983-11643002 | Msa0238230:three_prime_UTR | 20.0% |
| !! | TATTCTTAGTTATATAAAGG+TGG | + | chr2_2:11643035-11643054 | Msa0238230:three_prime_UTR | 20.0% |
| !! | ATACATGATGACTAATATTT+AGG | - | chr2_2:11643094-11643113 | None:intergenic | 20.0% |
| !! | AAATATTAGTCATCATGTAT+AGG | + | chr2_2:11643094-11643113 | Msa0238230:three_prime_UTR | 20.0% |
| ! | TGTTTGATAGAATTCTTGTT+AGG | - | chr2_2:11642864-11642883 | None:intergenic | 25.0% |
| ! | AATTCTATCAAACAGTGAAA+TGG | + | chr2_2:11642870-11642889 | Msa0238230:exon | 25.0% |
| ! | TGTTTGATAGAATTCTTGTT+AGG | - | chr2_2:11642864-11642883 | None:intergenic | 25.0% |
| ! | AATTCTATCAAACAGTGAAA+TGG | + | chr2_2:11642870-11642889 | Msa0238230:exon | 25.0% |
| !!! | ATTTTTTGCAAACTTGAGCA+AGG | + | chr2_2:11642289-11642308 | Msa0238230:CDS | 30.0% |
| CTTCATCTTCATCCATTATT+AGG | - | chr2_2:11642325-11642344 | None:intergenic | 30.0% | |
| TGAGGTGCATGTATTAATTA+AGG | - | chr2_2:11642403-11642422 | None:intergenic | 30.0% | |
| CATAATTGAAACTTGGAAGA+AGG | - | chr2_2:11642469-11642488 | None:intergenic | 30.0% | |
| TCATAGAAGAAATCAAACTG+TGG | - | chr2_2:11642711-11642730 | None:intergenic | 30.0% | |
| TTGATTAGAAGAGAAAGCAA+AGG | + | chr2_2:11642738-11642757 | Msa0238230:CDS | 30.0% | |
| TATAAAGGTGGATATGTACA+TGG | + | chr2_2:11643047-11643066 | Msa0238230:three_prime_UTR | 30.0% | |
| ATAAAGGTGGATATGTACAT+GGG | + | chr2_2:11643048-11643067 | Msa0238230:three_prime_UTR | 30.0% | |
| TATTAGTCATCATGTATAGG+AGG | + | chr2_2:11643097-11643116 | Msa0238230:three_prime_UTR | 30.0% | |
| !!! | ATTTTTTGCAAACTTGAGCA+AGG | + | chr2_2:11642289-11642308 | Msa0238230:CDS | 30.0% |
| CTTCATCTTCATCCATTATT+AGG | - | chr2_2:11642325-11642344 | None:intergenic | 30.0% | |
| TGAGGTGCATGTATTAATTA+AGG | - | chr2_2:11642403-11642422 | None:intergenic | 30.0% | |
| CATAATTGAAACTTGGAAGA+AGG | - | chr2_2:11642469-11642488 | None:intergenic | 30.0% | |
| TCATAGAAGAAATCAAACTG+TGG | - | chr2_2:11642711-11642730 | None:intergenic | 30.0% | |
| TTGATTAGAAGAGAAAGCAA+AGG | + | chr2_2:11642738-11642757 | Msa0238230:CDS | 30.0% | |
| TATAAAGGTGGATATGTACA+TGG | + | chr2_2:11643047-11643066 | Msa0238230:three_prime_UTR | 30.0% | |
| ATAAAGGTGGATATGTACAT+GGG | + | chr2_2:11643048-11643067 | Msa0238230:three_prime_UTR | 30.0% | |
| TATTAGTCATCATGTATAGG+AGG | + | chr2_2:11643097-11643116 | Msa0238230:three_prime_UTR | 30.0% | |
| AATTAACATGGAGGTTGCTT+TGG | + | chr2_2:11642250-11642269 | Msa0238230:exon | 35.0% | |
| GGAAATTGCTCTCCTAATAA+TGG | + | chr2_2:11642310-11642329 | Msa0238230:CDS | 35.0% | |
| GGTGCATGTATTAATTAAGG+AGG | - | chr2_2:11642400-11642419 | None:intergenic | 35.0% | |
| TTTATTTGTGCATGGCAGTT+CGG | + | chr2_2:11642506-11642525 | Msa0238230:intron | 35.0% | |
| TAGAAGAAATCAAACTGTGG+AGG | - | chr2_2:11642708-11642727 | None:intergenic | 35.0% | |
| ! | ATCACACAGCTTTAACTAGA+AGG | - | chr2_2:11642912-11642931 | None:intergenic | 35.0% |
| GCCTTTAGGCAATAATTGTA+TGG | + | chr2_2:11642997-11643016 | Msa0238230:three_prime_UTR | 35.0% | |
| GCCATACAATTATTGCCTAA+AGG | - | chr2_2:11643001-11643020 | None:intergenic | 35.0% | |
| AATTAACATGGAGGTTGCTT+TGG | + | chr2_2:11642250-11642269 | Msa0238230:exon | 35.0% | |
| GGAAATTGCTCTCCTAATAA+TGG | + | chr2_2:11642310-11642329 | Msa0238230:CDS | 35.0% | |
| GGTGCATGTATTAATTAAGG+AGG | - | chr2_2:11642400-11642419 | None:intergenic | 35.0% | |
| TTTATTTGTGCATGGCAGTT+CGG | + | chr2_2:11642506-11642525 | Msa0238230:intron | 35.0% | |
| TAGAAGAAATCAAACTGTGG+AGG | - | chr2_2:11642708-11642727 | None:intergenic | 35.0% | |
| ! | ATCACACAGCTTTAACTAGA+AGG | - | chr2_2:11642912-11642931 | None:intergenic | 35.0% |
| GCCTTTAGGCAATAATTGTA+TGG | + | chr2_2:11642997-11643016 | Msa0238230:three_prime_UTR | 35.0% | |
| GCCATACAATTATTGCCTAA+AGG | - | chr2_2:11643001-11643020 | None:intergenic | 35.0% | |
| AAGAAATCAAACTGTGGAGG+AGG | - | chr2_2:11642705-11642724 | None:intergenic | 40.0% | |
| AGAAGAGAAAGCAAAGGAAC+AGG | + | chr2_2:11642744-11642763 | Msa0238230:CDS | 40.0% | |
| !! | TTCTTGGTTGTGTTGCTTGT+GGG | - | chr2_2:11642779-11642798 | None:intergenic | 40.0% |
| GTATAGGAGGATTCAACCAA+AGG | + | chr2_2:11643110-11643129 | Msa0238230:three_prime_UTR | 40.0% | |
| AAGAAATCAAACTGTGGAGG+AGG | - | chr2_2:11642705-11642724 | None:intergenic | 40.0% | |
| AGAAGAGAAAGCAAAGGAAC+AGG | + | chr2_2:11642744-11642763 | Msa0238230:CDS | 40.0% | |
| !! | TTCTTGGTTGTGTTGCTTGT+GGG | - | chr2_2:11642779-11642798 | None:intergenic | 40.0% |
| GTATAGGAGGATTCAACCAA+AGG | + | chr2_2:11643110-11643129 | Msa0238230:three_prime_UTR | 40.0% | |
| !! | CATGGAGGTTGCTTTGGAAA+TGG | + | chr2_2:11642256-11642275 | Msa0238230:exon | 45.0% |
| ! | TTTGCAGGTTGAGAAGCAAG+TGG | - | chr2_2:11642350-11642369 | None:intergenic | 45.0% |
| TCAACCTGCAAACTCTCTTC+AGG | + | chr2_2:11642358-11642377 | Msa0238230:CDS | 45.0% | |
| AGAACCTGAAGAGAGTTTGC+AGG | - | chr2_2:11642365-11642384 | None:intergenic | 45.0% | |
| !! | CTTCTTGGTTGTGTTGCTTG+TGG | - | chr2_2:11642780-11642799 | None:intergenic | 45.0% |
| CACAAGCAACACAACCAAGA+AGG | + | chr2_2:11642778-11642797 | Msa0238230:CDS | 45.0% | |
| AAGCAACACAACCAAGAAGG+AGG | + | chr2_2:11642781-11642800 | Msa0238230:CDS | 45.0% | |
| CCTTTCTTGTACCTCCTTCT+TGG | - | chr2_2:11642795-11642814 | None:intergenic | 45.0% | |
| ! | CCAAGAAGGAGGTACAAGAA+AGG | + | chr2_2:11642792-11642811 | Msa0238230:CDS | 45.0% |
| AGTGGAGGTTGCAAAACCTT+TGG | - | chr2_2:11643129-11643148 | None:intergenic | 45.0% | |
| CAAAATGCTTGTCCACACAG+TGG | - | chr2_2:11643147-11643166 | None:intergenic | 45.0% | |
| ! | CTGTGTGGACAAGCATTTTG+TGG | + | chr2_2:11643147-11643166 | Msa0238230:three_prime_UTR | 45.0% |
| !! | CATGGAGGTTGCTTTGGAAA+TGG | + | chr2_2:11642256-11642275 | Msa0238230:exon | 45.0% |
| ! | TTTGCAGGTTGAGAAGCAAG+TGG | - | chr2_2:11642350-11642369 | None:intergenic | 45.0% |
| TCAACCTGCAAACTCTCTTC+AGG | + | chr2_2:11642358-11642377 | Msa0238230:CDS | 45.0% | |
| AGAACCTGAAGAGAGTTTGC+AGG | - | chr2_2:11642365-11642384 | None:intergenic | 45.0% | |
| !! | CTTCTTGGTTGTGTTGCTTG+TGG | - | chr2_2:11642780-11642799 | None:intergenic | 45.0% |
| CACAAGCAACACAACCAAGA+AGG | + | chr2_2:11642778-11642797 | Msa0238230:CDS | 45.0% | |
| AAGCAACACAACCAAGAAGG+AGG | + | chr2_2:11642781-11642800 | Msa0238230:CDS | 45.0% | |
| CCTTTCTTGTACCTCCTTCT+TGG | - | chr2_2:11642795-11642814 | None:intergenic | 45.0% | |
| ! | CCAAGAAGGAGGTACAAGAA+AGG | + | chr2_2:11642792-11642811 | Msa0238230:CDS | 45.0% |
| AGTGGAGGTTGCAAAACCTT+TGG | - | chr2_2:11643129-11643148 | None:intergenic | 45.0% | |
| CAAAATGCTTGTCCACACAG+TGG | - | chr2_2:11643147-11643166 | None:intergenic | 45.0% | |
| ! | CTGTGTGGACAAGCATTTTG+TGG | + | chr2_2:11643147-11643166 | Msa0238230:three_prime_UTR | 45.0% |
| ATTTGTGCATGGCAGTTCGG+TGG | + | chr2_2:11642509-11642528 | Msa0238230:intron | 50.0% | |
| ! | GTTTTGCAACCTCCACTGTG+TGG | + | chr2_2:11643132-11643151 | Msa0238230:three_prime_UTR | 50.0% |
| AATGCTTGTCCACACAGTGG+AGG | - | chr2_2:11643144-11643163 | None:intergenic | 50.0% | |
| ATTTGTGCATGGCAGTTCGG+TGG | + | chr2_2:11642509-11642528 | Msa0238230:intron | 50.0% | |
| ! | GTTTTGCAACCTCCACTGTG+TGG | + | chr2_2:11643132-11643151 | Msa0238230:three_prime_UTR | 50.0% |
| AATGCTTGTCCACACAGTGG+AGG | - | chr2_2:11643144-11643163 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 11642163 | 11643238 | 11642163 | ID=Msa0238230;Name=Msa0238230 |
| chr2_2 | mRNA | 11642163 | 11643238 | 11642163 | ID=Msa0238230-mRNA-1;Parent=Msa0238230;Name=Msa0238230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=94|1|1|1|1|1|2|351|109 |
| chr2_2 | exon | 11642163 | 11642379 | 11642163 | ID=Msa0238230-mRNA-1:exon:4986;Parent=Msa0238230-mRNA-1 |
| chr2_2 | exon | 11642681 | 11643238 | 11642681 | ID=Msa0238230-mRNA-1:exon:4987;Parent=Msa0238230-mRNA-1 |
| chr2_2 | five_prime_UTR | 11642163 | 11642256 | 11642163 | ID=Msa0238230-mRNA-1:five_prime_utr;Parent=Msa0238230-mRNA-1 |
| chr2_2 | CDS | 11642257 | 11642379 | 11642257 | ID=Msa0238230-mRNA-1:cds;Parent=Msa0238230-mRNA-1 |
| chr2_2 | CDS | 11642681 | 11642887 | 11642681 | ID=Msa0238230-mRNA-1:cds;Parent=Msa0238230-mRNA-1 |
| chr2_2 | three_prime_UTR | 11642888 | 11643238 | 11642888 | ID=Msa0238230-mRNA-1:three_prime_utr;Parent=Msa0238230-mRNA-1 |
| Gene Sequence |
| Protein sequence |
