AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0196340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0196340 MtrunA17_Chr2g0287961 99.130 115 1 0 1 115 1 115 3.54e-81 233
Msa0196340 MtrunA17_Chr4g0074501 71.296 108 29 2 1 106 1 108 5.12e-53 162
Msa0196340 MtrunA17_Chr0c30g0494111 68.657 67 19 2 42 106 1 67 8.39e-27 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0196340 AT5G19190.1 43.810 105 48 4 1 98 1 101 1.71e-17 73.6
Msa0196340 AT3G06070.1 42.045 88 46 3 1 83 1 88 8.79e-16 68.9

Find 39 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTCTTGTACCTCCTTCT+TGG 0.149558 2_1:-13522397 None:intergenic
CTAGAAACTAAATACAATTT+TGG 0.181446 2_1:+13521806 Msa0196340:five_prime_UTR
CTTCATCTTCATCCATTATT+AGG 0.195790 2_1:-13521925 None:intergenic
ATTTAGGTGTATATAATATA+TGG 0.250639 2_1:-13522698 None:intergenic
TTAATATCATTAACAGAAAA+AGG 0.277524 2_1:-13522820 None:intergenic
TAATTAATTCTTTAGCCTTT+AGG 0.283220 2_1:+13522606 Msa0196340:three_prime_UTR
TTCTATTCTTATTTATATAA+AGG 0.283430 2_1:+13522655 Msa0196340:three_prime_UTR
ATACACGATGACTAATATTT+AGG 0.294719 2_1:-13522714 None:intergenic
TTCTTGGCTGTGTTGCTTGT+GGG 0.315774 2_1:-13522381 None:intergenic
GGAAATTGCTCTCCTAATAA+TGG 0.320698 2_1:+13521913 Msa0196340:CDS
TCAACCTGCAAACTCTCTTC+AGG 0.344380 2_1:+13521961 Msa0196340:CDS
AATTAACATGGAGGTTGCTT+TGG 0.348717 2_1:+13521853 Msa0196340:exon
CATGGAGGTTGCTTTGGAAA+TGG 0.372075 2_1:+13521859 Msa0196340:exon
TGTTTGATAGAATTCTTGTT+AGG 0.386930 2_1:-13522484 None:intergenic
GCCTTTAGGCAATAATTGTA+TGG 0.401046 2_1:+13522620 Msa0196340:three_prime_UTR
AGAAGAGAAAGCAAAGGAAC+AGG 0.411669 2_1:+13522349 Msa0196340:CDS
CTTCTTGGCTGTGTTGCTTG+TGG 0.415079 2_1:-13522382 None:intergenic
ATCACACAGCTTTAACTAGA+AGG 0.419335 2_1:-13522532 None:intergenic
AGAACCTGAAGAGAGTTTGC+AGG 0.436707 2_1:-13521965 None:intergenic
AGTGGAGGTTGCAAAACCTT+TGG 0.437819 2_1:-13522749 None:intergenic
AAATATTAGTCATCGTGTAT+AGG 0.451262 2_1:+13522717 Msa0196340:three_prime_UTR
AATTCTATCAAACAGTGAAA+TGG 0.469397 2_1:+13522493 Msa0196340:exon
GCCATACAATTATTGCCTAA+AGG 0.474343 2_1:-13522621 None:intergenic
CACAAGCAACACAGCCAAGA+AGG 0.493229 2_1:+13522383 Msa0196340:CDS
TATAAAGGTGGATATGTACA+TGG 0.524484 2_1:+13522670 Msa0196340:three_prime_UTR
AAGAAATCAAACTGTGGAGG+AGG 0.531737 2_1:-13522307 None:intergenic
TATTCTTATTTATATAAAGG+TGG 0.546547 2_1:+13522658 Msa0196340:three_prime_UTR
CCAAGAAGGAGGTACAAGAA+AGG 0.549607 2_1:+13522397 Msa0196340:CDS
TATTAGTCATCGTGTATAGG+AGG 0.579038 2_1:+13522720 Msa0196340:three_prime_UTR
GTATAGGAGGATTCAACCAA+AGG 0.594707 2_1:+13522733 Msa0196340:three_prime_UTR
TTGATTAGAAGAGAAAGCAA+AGG 0.610534 2_1:+13522343 Msa0196340:CDS
TTTGCAGGTTGAGAAGCAAG+TGG 0.620205 2_1:-13521950 None:intergenic
ATAAAGGTGGATATGTACAT+GGG 0.623401 2_1:+13522671 Msa0196340:three_prime_UTR
TAGAAGAAATCAAACTGTGG+AGG 0.634181 2_1:-13522310 None:intergenic
TTTATATTGAATTAACATGG+AGG 0.678774 2_1:+13521844 Msa0196340:exon
AAGCAACACAGCCAAGAAGG+AGG 0.682199 2_1:+13522386 Msa0196340:CDS
AATGCTTGTCCACACAGTGG+AGG 0.692722 2_1:-13522764 None:intergenic
CAAAATGCTTGTCCACACAG+TGG 0.720567 2_1:-13522767 None:intergenic
TCATAGAAGAAATCAAACTG+TGG 0.762193 2_1:-13522313 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAACTAATTTTATTTTGT+AGG + chr2_1:13522264-13522283 Msa0196340:intron 10.0%
!!! TTCTATTCTTATTTATATAA+AGG + chr2_1:13522655-13522674 Msa0196340:three_prime_UTR 10.0%
!!! TTAAACTAATTTTATTTTGT+AGG + chr2_1:13522264-13522283 Msa0196340:intron 10.0%
!!! TTCTATTCTTATTTATATAA+AGG + chr2_1:13522655-13522674 Msa0196340:three_prime_UTR 10.0%
!!! TCTTTTATATTGAATTAACA+TGG + chr2_1:13521841-13521860 Msa0196340:exon 15.0%
!!! TATTCTTATTTATATAAAGG+TGG + chr2_1:13522658-13522677 Msa0196340:three_prime_UTR 15.0%
!! ATTTAGGTGTATATAATATA+TGG - chr2_1:13522701-13522720 None:intergenic 15.0%
!! TTAATATCATTAACAGAAAA+AGG - chr2_1:13522823-13522842 None:intergenic 15.0%
!!! TCTTTTATATTGAATTAACA+TGG + chr2_1:13521841-13521860 Msa0196340:exon 15.0%
!!! TATTCTTATTTATATAAAGG+TGG + chr2_1:13522658-13522677 Msa0196340:three_prime_UTR 15.0%
!! ATTTAGGTGTATATAATATA+TGG - chr2_1:13522701-13522720 None:intergenic 15.0%
!! TTAATATCATTAACAGAAAA+AGG - chr2_1:13522823-13522842 None:intergenic 15.0%
!!! CTAGAAACTAAATACAATTT+TGG + chr2_1:13521806-13521825 Msa0196340:five_prime_UTR 20.0%
!! TTTATATTGAATTAACATGG+AGG + chr2_1:13521844-13521863 Msa0196340:exon 20.0%
!! TATATTAGTATGTAACTATG+AGG - chr2_1:13522025-13522044 None:intergenic 20.0%
!! AAAATGTCATAATTGAAACT+TGG - chr2_1:13522080-13522099 None:intergenic 20.0%
!! TTAAAGAATTTATTTGTGCA+TGG + chr2_1:13522102-13522121 Msa0196340:intron 20.0%
!!! TAATTAATTCTTTAGCCTTT+AGG + chr2_1:13522606-13522625 Msa0196340:three_prime_UTR 20.0%
!!! CTAGAAACTAAATACAATTT+TGG + chr2_1:13521806-13521825 Msa0196340:five_prime_UTR 20.0%
!! TTTATATTGAATTAACATGG+AGG + chr2_1:13521844-13521863 Msa0196340:exon 20.0%
!! TATATTAGTATGTAACTATG+AGG - chr2_1:13522025-13522044 None:intergenic 20.0%
!! AAAATGTCATAATTGAAACT+TGG - chr2_1:13522080-13522099 None:intergenic 20.0%
!! TTAAAGAATTTATTTGTGCA+TGG + chr2_1:13522102-13522121 Msa0196340:intron 20.0%
!!! TAATTAATTCTTTAGCCTTT+AGG + chr2_1:13522606-13522625 Msa0196340:three_prime_UTR 20.0%
!! ATTTTGCATATAGAGAAGAA+GGG - chr2_1:13522236-13522255 None:intergenic 25.0%
!! TATTTTGCATATAGAGAAGA+AGG - chr2_1:13522237-13522256 None:intergenic 25.0%
! TGTTTGATAGAATTCTTGTT+AGG - chr2_1:13522487-13522506 None:intergenic 25.0%
! AATTCTATCAAACAGTGAAA+TGG + chr2_1:13522493-13522512 Msa0196340:exon 25.0%
! ATACACGATGACTAATATTT+AGG - chr2_1:13522717-13522736 None:intergenic 25.0%
! AAATATTAGTCATCGTGTAT+AGG + chr2_1:13522717-13522736 Msa0196340:three_prime_UTR 25.0%
!! ATTTTGCATATAGAGAAGAA+GGG - chr2_1:13522236-13522255 None:intergenic 25.0%
!! TATTTTGCATATAGAGAAGA+AGG - chr2_1:13522237-13522256 None:intergenic 25.0%
! TGTTTGATAGAATTCTTGTT+AGG - chr2_1:13522487-13522506 None:intergenic 25.0%
! AATTCTATCAAACAGTGAAA+TGG + chr2_1:13522493-13522512 Msa0196340:exon 25.0%
! ATACACGATGACTAATATTT+AGG - chr2_1:13522717-13522736 None:intergenic 25.0%
! AAATATTAGTCATCGTGTAT+AGG + chr2_1:13522717-13522736 Msa0196340:three_prime_UTR 25.0%
!!! ATTTTTTGCAAACTTGAGCA+AGG + chr2_1:13521892-13521911 Msa0196340:CDS 30.0%
CTTCATCTTCATCCATTATT+AGG - chr2_1:13521928-13521947 None:intergenic 30.0%
GTGCATGTATTTAATTAAGG+AGG - chr2_1:13522003-13522022 None:intergenic 30.0%
GAGGTGCATGTATTTAATTA+AGG - chr2_1:13522006-13522025 None:intergenic 30.0%
CATAATTGAAACTTGGAAGA+AGG - chr2_1:13522073-13522092 None:intergenic 30.0%
TCATAGAAGAAATCAAACTG+TGG - chr2_1:13522316-13522335 None:intergenic 30.0%
TTGATTAGAAGAGAAAGCAA+AGG + chr2_1:13522343-13522362 Msa0196340:CDS 30.0%
TATAAAGGTGGATATGTACA+TGG + chr2_1:13522670-13522689 Msa0196340:three_prime_UTR 30.0%
ATAAAGGTGGATATGTACAT+GGG + chr2_1:13522671-13522690 Msa0196340:three_prime_UTR 30.0%
!!! ATTTTTTGCAAACTTGAGCA+AGG + chr2_1:13521892-13521911 Msa0196340:CDS 30.0%
CTTCATCTTCATCCATTATT+AGG - chr2_1:13521928-13521947 None:intergenic 30.0%
GTGCATGTATTTAATTAAGG+AGG - chr2_1:13522003-13522022 None:intergenic 30.0%
GAGGTGCATGTATTTAATTA+AGG - chr2_1:13522006-13522025 None:intergenic 30.0%
CATAATTGAAACTTGGAAGA+AGG - chr2_1:13522073-13522092 None:intergenic 30.0%
TCATAGAAGAAATCAAACTG+TGG - chr2_1:13522316-13522335 None:intergenic 30.0%
TTGATTAGAAGAGAAAGCAA+AGG + chr2_1:13522343-13522362 Msa0196340:CDS 30.0%
TATAAAGGTGGATATGTACA+TGG + chr2_1:13522670-13522689 Msa0196340:three_prime_UTR 30.0%
ATAAAGGTGGATATGTACAT+GGG + chr2_1:13522671-13522690 Msa0196340:three_prime_UTR 30.0%
AATTAACATGGAGGTTGCTT+TGG + chr2_1:13521853-13521872 Msa0196340:exon 35.0%
GGAAATTGCTCTCCTAATAA+TGG + chr2_1:13521913-13521932 Msa0196340:CDS 35.0%
TTTATTTGTGCATGGCAGTT+CGG + chr2_1:13522110-13522129 Msa0196340:intron 35.0%
TAGAAGAAATCAAACTGTGG+AGG - chr2_1:13522313-13522332 None:intergenic 35.0%
! ATCACACAGCTTTAACTAGA+AGG - chr2_1:13522535-13522554 None:intergenic 35.0%
GCCTTTAGGCAATAATTGTA+TGG + chr2_1:13522620-13522639 Msa0196340:three_prime_UTR 35.0%
GCCATACAATTATTGCCTAA+AGG - chr2_1:13522624-13522643 None:intergenic 35.0%
TATTAGTCATCGTGTATAGG+AGG + chr2_1:13522720-13522739 Msa0196340:three_prime_UTR 35.0%
AATTAACATGGAGGTTGCTT+TGG + chr2_1:13521853-13521872 Msa0196340:exon 35.0%
GGAAATTGCTCTCCTAATAA+TGG + chr2_1:13521913-13521932 Msa0196340:CDS 35.0%
TTTATTTGTGCATGGCAGTT+CGG + chr2_1:13522110-13522129 Msa0196340:intron 35.0%
TAGAAGAAATCAAACTGTGG+AGG - chr2_1:13522313-13522332 None:intergenic 35.0%
! ATCACACAGCTTTAACTAGA+AGG - chr2_1:13522535-13522554 None:intergenic 35.0%
GCCTTTAGGCAATAATTGTA+TGG + chr2_1:13522620-13522639 Msa0196340:three_prime_UTR 35.0%
GCCATACAATTATTGCCTAA+AGG - chr2_1:13522624-13522643 None:intergenic 35.0%
TATTAGTCATCGTGTATAGG+AGG + chr2_1:13522720-13522739 Msa0196340:three_prime_UTR 35.0%
AAGAAATCAAACTGTGGAGG+AGG - chr2_1:13522310-13522329 None:intergenic 40.0%
AGAAGAGAAAGCAAAGGAAC+AGG + chr2_1:13522349-13522368 Msa0196340:CDS 40.0%
GTATAGGAGGATTCAACCAA+AGG + chr2_1:13522733-13522752 Msa0196340:three_prime_UTR 40.0%
AAGAAATCAAACTGTGGAGG+AGG - chr2_1:13522310-13522329 None:intergenic 40.0%
AGAAGAGAAAGCAAAGGAAC+AGG + chr2_1:13522349-13522368 Msa0196340:CDS 40.0%
GTATAGGAGGATTCAACCAA+AGG + chr2_1:13522733-13522752 Msa0196340:three_prime_UTR 40.0%
!! CATGGAGGTTGCTTTGGAAA+TGG + chr2_1:13521859-13521878 Msa0196340:exon 45.0%
! TTTGCAGGTTGAGAAGCAAG+TGG - chr2_1:13521953-13521972 None:intergenic 45.0%
TCAACCTGCAAACTCTCTTC+AGG + chr2_1:13521961-13521980 Msa0196340:CDS 45.0%
AGAACCTGAAGAGAGTTTGC+AGG - chr2_1:13521968-13521987 None:intergenic 45.0%
!! TTCTTGGCTGTGTTGCTTGT+GGG - chr2_1:13522384-13522403 None:intergenic 45.0%
CCTTTCTTGTACCTCCTTCT+TGG - chr2_1:13522400-13522419 None:intergenic 45.0%
! CCAAGAAGGAGGTACAAGAA+AGG + chr2_1:13522397-13522416 Msa0196340:CDS 45.0%
AGTGGAGGTTGCAAAACCTT+TGG - chr2_1:13522752-13522771 None:intergenic 45.0%
CAAAATGCTTGTCCACACAG+TGG - chr2_1:13522770-13522789 None:intergenic 45.0%
! CTGTGTGGACAAGCATTTTG+TGG + chr2_1:13522770-13522789 Msa0196340:three_prime_UTR 45.0%
!! CATGGAGGTTGCTTTGGAAA+TGG + chr2_1:13521859-13521878 Msa0196340:exon 45.0%
! TTTGCAGGTTGAGAAGCAAG+TGG - chr2_1:13521953-13521972 None:intergenic 45.0%
TCAACCTGCAAACTCTCTTC+AGG + chr2_1:13521961-13521980 Msa0196340:CDS 45.0%
AGAACCTGAAGAGAGTTTGC+AGG - chr2_1:13521968-13521987 None:intergenic 45.0%
!! TTCTTGGCTGTGTTGCTTGT+GGG - chr2_1:13522384-13522403 None:intergenic 45.0%
CCTTTCTTGTACCTCCTTCT+TGG - chr2_1:13522400-13522419 None:intergenic 45.0%
! CCAAGAAGGAGGTACAAGAA+AGG + chr2_1:13522397-13522416 Msa0196340:CDS 45.0%
AGTGGAGGTTGCAAAACCTT+TGG - chr2_1:13522752-13522771 None:intergenic 45.0%
CAAAATGCTTGTCCACACAG+TGG - chr2_1:13522770-13522789 None:intergenic 45.0%
! CTGTGTGGACAAGCATTTTG+TGG + chr2_1:13522770-13522789 Msa0196340:three_prime_UTR 45.0%
ATTTGTGCATGGCAGTTCGG+TGG + chr2_1:13522113-13522132 Msa0196340:intron 50.0%
!! CTTCTTGGCTGTGTTGCTTG+TGG - chr2_1:13522385-13522404 None:intergenic 50.0%
CACAAGCAACACAGCCAAGA+AGG + chr2_1:13522383-13522402 Msa0196340:CDS 50.0%
AAGCAACACAGCCAAGAAGG+AGG + chr2_1:13522386-13522405 Msa0196340:CDS 50.0%
! GTTTTGCAACCTCCACTGTG+TGG + chr2_1:13522755-13522774 Msa0196340:three_prime_UTR 50.0%
AATGCTTGTCCACACAGTGG+AGG - chr2_1:13522767-13522786 None:intergenic 50.0%
ATTTGTGCATGGCAGTTCGG+TGG + chr2_1:13522113-13522132 Msa0196340:intron 50.0%
!! CTTCTTGGCTGTGTTGCTTG+TGG - chr2_1:13522385-13522404 None:intergenic 50.0%
CACAAGCAACACAGCCAAGA+AGG + chr2_1:13522383-13522402 Msa0196340:CDS 50.0%
AAGCAACACAGCCAAGAAGG+AGG + chr2_1:13522386-13522405 Msa0196340:CDS 50.0%
! GTTTTGCAACCTCCACTGTG+TGG + chr2_1:13522755-13522774 Msa0196340:three_prime_UTR 50.0%
AATGCTTGTCCACACAGTGG+AGG - chr2_1:13522767-13522786 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 13521766 13522861 13521766 ID=Msa0196340;Name=Msa0196340
chr2_1 mRNA 13521766 13522861 13521766 ID=Msa0196340-mRNA-1;Parent=Msa0196340;Name=Msa0196340-mRNA-1;_AED=0.04;_eAED=0.04;_QI=94|1|1|1|1|1|2|351|115
chr2_1 exon 13521766 13521982 13521766 ID=Msa0196340-mRNA-1:exon:5822;Parent=Msa0196340-mRNA-1
chr2_1 exon 13522286 13522861 13522286 ID=Msa0196340-mRNA-1:exon:5823;Parent=Msa0196340-mRNA-1
chr2_1 five_prime_UTR 13521766 13521859 13521766 ID=Msa0196340-mRNA-1:five_prime_utr;Parent=Msa0196340-mRNA-1
chr2_1 CDS 13521860 13521982 13521860 ID=Msa0196340-mRNA-1:cds;Parent=Msa0196340-mRNA-1
chr2_1 CDS 13522286 13522510 13522286 ID=Msa0196340-mRNA-1:cds;Parent=Msa0196340-mRNA-1
chr2_1 three_prime_UTR 13522511 13522861 13522511 ID=Msa0196340-mRNA-1:three_prime_utr;Parent=Msa0196340-mRNA-1
Gene Sequence

>Msa0196340

ATGGAGGTTGCTTTGGAAATGGAAGATGATTTATTTTTTGCAAACTTGAGCAAGGAAATTGCTCTCCTAATAATGGATGAAGATGAAGATCCACTTGCTTCTCAACCTGCAAACTCTCTTCAGGATTTCTCTAGAGCAATTCATCCTCCTCCACAGTTTGATTTCTTCTATGAGCAAGCTTTGATTAGAAGAGAAAGCAAAGGAACAGGAGTGTTTATCCCACAAGCAACACAGCCAAGAAGGAGGTACAAGAAAGGAAGATCTAATTCATTCTCAAAATATCAAAAGCAGTCTCAAGACACAAGAATGATTTCTCAAGTTCCTAACAAGAATTCTATCAAACAGTGA

Protein sequence

>Msa0196340

MEVALEMEDDLFFANLSKEIALLIMDEDEDPLASQPANSLQDFSRAIHPPPQFDFFYEQALIRRESKGTGVFIPQATQPRRRYKKGRSNSFSKYQKQSQDTRMISQVPNKNSIKQ*