AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0899900


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCCCTTTGCCTAGACTTT+AGG 0.335492 6_1:+95324896 Msa0899900:CDS
CCAATTGTTGAGCTATTCAT+TGG 0.390337 6_1:+95324384 Msa0899900:CDS
AACGTCGCCTCAGGTAACTT+TGG 0.396304 6_1:+95324867 Msa0899900:CDS
CCAATGAATAGCTCAACAAT+TGG 0.434045 6_1:-95324384 None:intergenic
TGCTTCAGGTAATGGCTACT+CGG 0.454119 6_1:+95324353 Msa0899900:intron
ACGTCGCCTCAGGTAACTTT+GGG 0.459659 6_1:+95324868 Msa0899900:CDS
TCGCCTAAAGTCTAGGCAAA+GGG 0.478044 6_1:-95324899 None:intergenic
TTGGTAGCATAAGACTTGCT+CGG 0.513928 6_1:+95324403 Msa0899900:CDS
TTAAGCGTCGCCTAAAGTCT+AGG 0.517572 6_1:-95324906 None:intergenic
TGGTAGCATAAGACTTGCTC+GGG 0.522565 6_1:+95324404 Msa0899900:CDS
GTCGCCTAAAGTCTAGGCAA+AGG 0.576462 6_1:-95324900 None:intergenic
GCTTCAGGTAATGGCTACTC+GGG 0.580207 6_1:+95324354 Msa0899900:intron
TAGCATAAGACTTGCTCGGG+TGG 0.628154 6_1:+95324407 Msa0899900:CDS
CGCCTAAAGTCTAGGCAAAG+GGG 0.662867 6_1:-95324898 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTTATAAATATGTGA+TGG + chr6_1:95324524-95324543 Msa0899900:intron 10.0%
!! ATTTATTTATAAATATGTGA+TGG + chr6_1:95324524-95324543 Msa0899900:intron 10.0%
!! ACATGAATAAAATAAAACAA+GGG - chr6_1:95323625-95323644 None:intergenic 15.0%
!! AACATGAATAAAATAAAACA+AGG - chr6_1:95323626-95323645 None:intergenic 15.0%
!!! AAAAAAACTTTTTAACATGA+TGG + chr6_1:95323928-95323947 Msa0899900:intron 15.0%
!! TATTATAAGTAGACATAAAA+CGG - chr6_1:95324287-95324306 None:intergenic 15.0%
!!! AAGAGTTTTTTTATATGTTA+AGG + chr6_1:95324736-95324755 Msa0899900:intron 15.0%
!!! AGAGTTTTTTTATATGTTAA+GGG + chr6_1:95324737-95324756 Msa0899900:intron 15.0%
!! ACATGAATAAAATAAAACAA+GGG - chr6_1:95323625-95323644 None:intergenic 15.0%
!! AACATGAATAAAATAAAACA+AGG - chr6_1:95323626-95323645 None:intergenic 15.0%
!!! AAAAAAACTTTTTAACATGA+TGG + chr6_1:95323928-95323947 Msa0899900:intron 15.0%
!! TATTATAAGTAGACATAAAA+CGG - chr6_1:95324287-95324306 None:intergenic 15.0%
!!! AAGAGTTTTTTTATATGTTA+AGG + chr6_1:95324736-95324755 Msa0899900:intron 15.0%
!!! AGAGTTTTTTTATATGTTAA+GGG + chr6_1:95324737-95324756 Msa0899900:intron 15.0%
!!! TTATTTATCTTCCTCTTTTT+TGG + chr6_1:95323889-95323908 Msa0899900:intron 20.0%
!! CAAATAATGCATTTATGTTA+AGG - chr6_1:95324062-95324081 None:intergenic 20.0%
!! TAAATCAAGTTCAAATTGAA+TGG - chr6_1:95324111-95324130 None:intergenic 20.0%
!! GATAGAGAATAATAATTCAT+TGG - chr6_1:95324688-95324707 None:intergenic 20.0%
!!! TTTTATTTATGATTAGGCAA+CGG + chr6_1:95324810-95324829 Msa0899900:intron 20.0%
!!! TTATTTATCTTCCTCTTTTT+TGG + chr6_1:95323889-95323908 Msa0899900:intron 20.0%
!! CAAATAATGCATTTATGTTA+AGG - chr6_1:95324062-95324081 None:intergenic 20.0%
!! TAAATCAAGTTCAAATTGAA+TGG - chr6_1:95324111-95324130 None:intergenic 20.0%
!! GATAGAGAATAATAATTCAT+TGG - chr6_1:95324688-95324707 None:intergenic 20.0%
!!! TTTTATTTATGATTAGGCAA+CGG + chr6_1:95324810-95324829 Msa0899900:intron 20.0%
! TCTGAGTAGAATACAAATTA+AGG - chr6_1:95323309-95323328 None:intergenic 25.0%
!! TTTATGTGGATTTTCTACTT+AGG + chr6_1:95323363-95323382 Msa0899900:intron 25.0%
! TGATATTCTCTCAAAATACA+AGG + chr6_1:95323524-95323543 Msa0899900:intron 25.0%
! TCTCAAAATACAAGGATTAT+TGG + chr6_1:95323532-95323551 Msa0899900:intron 25.0%
! AAAATACAAGGATTATTGGT+TGG + chr6_1:95323536-95323555 Msa0899900:intron 25.0%
!!! TGTGTTTTTAACCAAAAAAG+AGG - chr6_1:95323903-95323922 None:intergenic 25.0%
!!! TTTTATTTAAGCACTAGCTT+AGG + chr6_1:95324000-95324019 Msa0899900:intron 25.0%
!! AATTTTATGAGCAAAAGACA+CGG - chr6_1:95324139-95324158 None:intergenic 25.0%
! TTCATGAAATTCATATCTGA+AGG - chr6_1:95324242-95324261 None:intergenic 25.0%
! CATAAAATAAGAGATTGATG+AGG - chr6_1:95324568-95324587 None:intergenic 25.0%
!! AAAATTTACAAAAAGCGTGT+TGG - chr6_1:95324641-95324660 None:intergenic 25.0%
! AAAAATTAGTGTGGAAAAGT+CGG + chr6_1:95324760-95324779 Msa0899900:intron 25.0%
!!! TTTTTAATTGTTGCGATATC+TGG - chr6_1:95324849-95324868 None:intergenic 25.0%
! TCTGAGTAGAATACAAATTA+AGG - chr6_1:95323309-95323328 None:intergenic 25.0%
!! TTTATGTGGATTTTCTACTT+AGG + chr6_1:95323363-95323382 Msa0899900:intron 25.0%
! TGATATTCTCTCAAAATACA+AGG + chr6_1:95323524-95323543 Msa0899900:intron 25.0%
! TCTCAAAATACAAGGATTAT+TGG + chr6_1:95323532-95323551 Msa0899900:intron 25.0%
! AAAATACAAGGATTATTGGT+TGG + chr6_1:95323536-95323555 Msa0899900:intron 25.0%
!!! TGTGTTTTTAACCAAAAAAG+AGG - chr6_1:95323903-95323922 None:intergenic 25.0%
!!! TTTTATTTAAGCACTAGCTT+AGG + chr6_1:95324000-95324019 Msa0899900:intron 25.0%
!! AATTTTATGAGCAAAAGACA+CGG - chr6_1:95324139-95324158 None:intergenic 25.0%
! TTCATGAAATTCATATCTGA+AGG - chr6_1:95324242-95324261 None:intergenic 25.0%
! CATAAAATAAGAGATTGATG+AGG - chr6_1:95324568-95324587 None:intergenic 25.0%
!! AAAATTTACAAAAAGCGTGT+TGG - chr6_1:95324641-95324660 None:intergenic 25.0%
! AAAAATTAGTGTGGAAAAGT+CGG + chr6_1:95324760-95324779 Msa0899900:intron 25.0%
!!! TTTTTAATTGTTGCGATATC+TGG - chr6_1:95324849-95324868 None:intergenic 25.0%
GCATAAATTCAGTTTACAAC+AGG - chr6_1:95323218-95323237 None:intergenic 30.0%
GTATTCTACTCAGAAATGTT+AGG + chr6_1:95323315-95323334 Msa0899900:intron 30.0%
! GATTCTTTCTGCTTTAGATA+TGG + chr6_1:95323435-95323454 Msa0899900:intron 30.0%
!! TACCTGAACTTGTTTTCTAA+AGG + chr6_1:95323670-95323689 Msa0899900:intron 30.0%
! ATTTTATGAGCAAAAGACAC+GGG - chr6_1:95324138-95324157 None:intergenic 30.0%
!! ACTGTTTTAAGTGCTTCAAA+CGG + chr6_1:95324471-95324490 Msa0899900:intron 30.0%
TGTTAAGGGAAAAATTAGTG+TGG + chr6_1:95324751-95324770 Msa0899900:intron 30.0%
GCATAAATTCAGTTTACAAC+AGG - chr6_1:95323218-95323237 None:intergenic 30.0%
GTATTCTACTCAGAAATGTT+AGG + chr6_1:95323315-95323334 Msa0899900:intron 30.0%
! GATTCTTTCTGCTTTAGATA+TGG + chr6_1:95323435-95323454 Msa0899900:intron 30.0%
!! TACCTGAACTTGTTTTCTAA+AGG + chr6_1:95323670-95323689 Msa0899900:intron 30.0%
! ATTTTATGAGCAAAAGACAC+GGG - chr6_1:95324138-95324157 None:intergenic 30.0%
!! ACTGTTTTAAGTGCTTCAAA+CGG + chr6_1:95324471-95324490 Msa0899900:intron 30.0%
TGTTAAGGGAAAAATTAGTG+TGG + chr6_1:95324751-95324770 Msa0899900:intron 30.0%
!!! AAGCTGCTTTTAGCTTTATG+TGG + chr6_1:95323349-95323368 Msa0899900:intron 35.0%
TAGATGAACACTGAACATGA+AGG + chr6_1:95323473-95323492 Msa0899900:intron 35.0%
! TGCCTTTAGAAAACAAGTTC+AGG - chr6_1:95323675-95323694 None:intergenic 35.0%
!!! GACTTGTTAGTACTGGTTTT+TGG + chr6_1:95323964-95323983 Msa0899900:intron 35.0%
ATGTTGTTATCACCATTCTC+AGG + chr6_1:95324083-95324102 Msa0899900:intron 35.0%
AATTGAATGGATCCTGAGAA+TGG - chr6_1:95324098-95324117 None:intergenic 35.0%
! ATTTGTTGCAGATGGTTTGA+GGG + chr6_1:95324168-95324187 Msa0899900:intron 35.0%
CATATCTGAAGGATGATGTT+AGG - chr6_1:95324231-95324250 None:intergenic 35.0%
CCAATGAATAGCTCAACAAT+TGG - chr6_1:95324387-95324406 None:intergenic 35.0%
CCAATTGTTGAGCTATTCAT+TGG + chr6_1:95324384-95324403 Msa0899900:CDS 35.0%
TACCAGACAATGTTACAACA+AGG + chr6_1:95324445-95324464 Msa0899900:intron 35.0%
ACCAGACAATGTTACAACAA+GGG + chr6_1:95324446-95324465 Msa0899900:intron 35.0%
!!! AAGCTGCTTTTAGCTTTATG+TGG + chr6_1:95323349-95323368 Msa0899900:intron 35.0%
TAGATGAACACTGAACATGA+AGG + chr6_1:95323473-95323492 Msa0899900:intron 35.0%
! TGCCTTTAGAAAACAAGTTC+AGG - chr6_1:95323675-95323694 None:intergenic 35.0%
!!! GACTTGTTAGTACTGGTTTT+TGG + chr6_1:95323964-95323983 Msa0899900:intron 35.0%
ATGTTGTTATCACCATTCTC+AGG + chr6_1:95324083-95324102 Msa0899900:intron 35.0%
AATTGAATGGATCCTGAGAA+TGG - chr6_1:95324098-95324117 None:intergenic 35.0%
! ATTTGTTGCAGATGGTTTGA+GGG + chr6_1:95324168-95324187 Msa0899900:intron 35.0%
CATATCTGAAGGATGATGTT+AGG - chr6_1:95324231-95324250 None:intergenic 35.0%
CCAATGAATAGCTCAACAAT+TGG - chr6_1:95324387-95324406 None:intergenic 35.0%
CCAATTGTTGAGCTATTCAT+TGG + chr6_1:95324384-95324403 Msa0899900:CDS 35.0%
TACCAGACAATGTTACAACA+AGG + chr6_1:95324445-95324464 Msa0899900:intron 35.0%
ACCAGACAATGTTACAACAA+GGG + chr6_1:95324446-95324465 Msa0899900:intron 35.0%
! AGGTGTCGTGTTATGTAGTT+TGG + chr6_1:95323383-95323402 Msa0899900:intron 40.0%
AACACAGAGTGTGTCGAAAA+CGG - chr6_1:95323708-95323727 None:intergenic 40.0%
!! TGTCGTAGACTTGTTAGTAC+TGG + chr6_1:95323957-95323976 Msa0899900:intron 40.0%
CTCAAACCATCTGCAACAAA+TGG - chr6_1:95324169-95324188 None:intergenic 40.0%
! CATTTGTTGCAGATGGTTTG+AGG + chr6_1:95324167-95324186 Msa0899900:intron 40.0%
TTGACTTCCTTTCATGCTTC+AGG + chr6_1:95324339-95324358 Msa0899900:intron 40.0%
TCCTTTCATGCTTCAGGTAA+TGG + chr6_1:95324345-95324364 Msa0899900:intron 40.0%
! TTGGTAGCATAAGACTTGCT+CGG + chr6_1:95324403-95324422 Msa0899900:CDS 40.0%
! TCCCTTGTTGTAACATTGTC+TGG - chr6_1:95324450-95324469 None:intergenic 40.0%
!! GTTTTAAGTGCTTCAAACGG+CGG + chr6_1:95324474-95324493 Msa0899900:intron 40.0%
TAGTGTGGAAAAGTCGGATA+TGG + chr6_1:95324766-95324785 Msa0899900:intron 40.0%
ACAATTAAAAACGTCGCCTC+AGG + chr6_1:95324858-95324877 Msa0899900:CDS 40.0%
! AGGTGTCGTGTTATGTAGTT+TGG + chr6_1:95323383-95323402 Msa0899900:intron 40.0%
AACACAGAGTGTGTCGAAAA+CGG - chr6_1:95323708-95323727 None:intergenic 40.0%
!! TGTCGTAGACTTGTTAGTAC+TGG + chr6_1:95323957-95323976 Msa0899900:intron 40.0%
CTCAAACCATCTGCAACAAA+TGG - chr6_1:95324169-95324188 None:intergenic 40.0%
! CATTTGTTGCAGATGGTTTG+AGG + chr6_1:95324167-95324186 Msa0899900:intron 40.0%
TTGACTTCCTTTCATGCTTC+AGG + chr6_1:95324339-95324358 Msa0899900:intron 40.0%
TCCTTTCATGCTTCAGGTAA+TGG + chr6_1:95324345-95324364 Msa0899900:intron 40.0%
! TTGGTAGCATAAGACTTGCT+CGG + chr6_1:95324403-95324422 Msa0899900:CDS 40.0%
! TCCCTTGTTGTAACATTGTC+TGG - chr6_1:95324450-95324469 None:intergenic 40.0%
!! GTTTTAAGTGCTTCAAACGG+CGG + chr6_1:95324474-95324493 Msa0899900:intron 40.0%
TAGTGTGGAAAAGTCGGATA+TGG + chr6_1:95324766-95324785 Msa0899900:intron 40.0%
ACAATTAAAAACGTCGCCTC+AGG + chr6_1:95324858-95324877 Msa0899900:CDS 40.0%
! CTCTTTGTGTTGCTGCATTC+AGG + chr6_1:95323819-95323838 Msa0899900:intron 45.0%
! GTGCTTCCATTTGTTGCAGA+TGG + chr6_1:95324160-95324179 Msa0899900:intron 45.0%
GATGATGTTAGGAGAGAGGT+TGG - chr6_1:95324220-95324239 None:intergenic 45.0%
GAAGGATGATGTTAGGAGAG+AGG - chr6_1:95324224-95324243 None:intergenic 45.0%
GCCATTACCTGAAGCATGAA+AGG - chr6_1:95324349-95324368 None:intergenic 45.0%
! TGCTTCAGGTAATGGCTACT+CGG + chr6_1:95324353-95324372 Msa0899900:intron 45.0%
! TGGTAGCATAAGACTTGCTC+GGG + chr6_1:95324404-95324423 Msa0899900:CDS 45.0%
! CGTTTTCCCAAAGTTACCTG+AGG - chr6_1:95324877-95324896 None:intergenic 45.0%
TCGCCTAAAGTCTAGGCAAA+GGG - chr6_1:95324902-95324921 None:intergenic 45.0%
! TTAAGCGTCGCCTAAAGTCT+AGG - chr6_1:95324909-95324928 None:intergenic 45.0%
! CTCTTTGTGTTGCTGCATTC+AGG + chr6_1:95323819-95323838 Msa0899900:intron 45.0%
! GTGCTTCCATTTGTTGCAGA+TGG + chr6_1:95324160-95324179 Msa0899900:intron 45.0%
GATGATGTTAGGAGAGAGGT+TGG - chr6_1:95324220-95324239 None:intergenic 45.0%
GAAGGATGATGTTAGGAGAG+AGG - chr6_1:95324224-95324243 None:intergenic 45.0%
GCCATTACCTGAAGCATGAA+AGG - chr6_1:95324349-95324368 None:intergenic 45.0%
! TGCTTCAGGTAATGGCTACT+CGG + chr6_1:95324353-95324372 Msa0899900:intron 45.0%
! TGGTAGCATAAGACTTGCTC+GGG + chr6_1:95324404-95324423 Msa0899900:CDS 45.0%
! CGTTTTCCCAAAGTTACCTG+AGG - chr6_1:95324877-95324896 None:intergenic 45.0%
TCGCCTAAAGTCTAGGCAAA+GGG - chr6_1:95324902-95324921 None:intergenic 45.0%
! TTAAGCGTCGCCTAAAGTCT+AGG - chr6_1:95324909-95324928 None:intergenic 45.0%
!!! TTATATTTTTATTTATGATT+AGG + chr6_1:95324804-95324823 Msa0899900:intron 5.0%
!!! TTATATTTTTATTTATGATT+AGG + chr6_1:95324804-95324823 Msa0899900:intron 5.0%
! GCTTCAGGTAATGGCTACTC+GGG + chr6_1:95324354-95324373 Msa0899900:intron 50.0%
! TAGCATAAGACTTGCTCGGG+TGG + chr6_1:95324407-95324426 Msa0899900:CDS 50.0%
GTGGAAAAGTCGGATATGGC+AGG + chr6_1:95324770-95324789 Msa0899900:intron 50.0%
AACGTCGCCTCAGGTAACTT+TGG + chr6_1:95324867-95324886 Msa0899900:CDS 50.0%
ACGTCGCCTCAGGTAACTTT+GGG + chr6_1:95324868-95324887 Msa0899900:CDS 50.0%
GTCCCCTTTGCCTAGACTTT+AGG + chr6_1:95324896-95324915 Msa0899900:CDS 50.0%
CGCCTAAAGTCTAGGCAAAG+GGG - chr6_1:95324901-95324920 None:intergenic 50.0%
GTCGCCTAAAGTCTAGGCAA+AGG - chr6_1:95324903-95324922 None:intergenic 50.0%
! GCTTCAGGTAATGGCTACTC+GGG + chr6_1:95324354-95324373 Msa0899900:intron 50.0%
! TAGCATAAGACTTGCTCGGG+TGG + chr6_1:95324407-95324426 Msa0899900:CDS 50.0%
GTGGAAAAGTCGGATATGGC+AGG + chr6_1:95324770-95324789 Msa0899900:intron 50.0%
AACGTCGCCTCAGGTAACTT+TGG + chr6_1:95324867-95324886 Msa0899900:CDS 50.0%
ACGTCGCCTCAGGTAACTTT+GGG + chr6_1:95324868-95324887 Msa0899900:CDS 50.0%
GTCCCCTTTGCCTAGACTTT+AGG + chr6_1:95324896-95324915 Msa0899900:CDS 50.0%
CGCCTAAAGTCTAGGCAAAG+GGG - chr6_1:95324901-95324920 None:intergenic 50.0%
GTCGCCTAAAGTCTAGGCAA+AGG - chr6_1:95324903-95324922 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 95323187 95324951 95323187 ID=Msa0899900;Name=Msa0899900
chr6_1 mRNA 95323187 95324951 95323187 ID=Msa0899900-mRNA-1;Parent=Msa0899900;Name=Msa0899900-mRNA-1;_AED=0.78;_eAED=0.79;_QI=0|0|0|0.5|0.33|0.25|4|0|59
chr6_1 exon 95323187 95323190 95323187 ID=Msa0899900-mRNA-1:exon:16430;Parent=Msa0899900-mRNA-1
chr6_1 exon 95324361 95324428 95324361 ID=Msa0899900-mRNA-1:exon:16431;Parent=Msa0899900-mRNA-1
chr6_1 exon 95324826 95324831 95324826 ID=Msa0899900-mRNA-1:exon:16432;Parent=Msa0899900-mRNA-1
chr6_1 exon 95324850 95324951 95324850 ID=Msa0899900-mRNA-1:exon:16433;Parent=Msa0899900-mRNA-1
chr6_1 CDS 95323187 95323190 95323187 ID=Msa0899900-mRNA-1:cds;Parent=Msa0899900-mRNA-1
chr6_1 CDS 95324361 95324428 95324361 ID=Msa0899900-mRNA-1:cds;Parent=Msa0899900-mRNA-1
chr6_1 CDS 95324826 95324831 95324826 ID=Msa0899900-mRNA-1:cds;Parent=Msa0899900-mRNA-1
chr6_1 CDS 95324850 95324951 95324850 ID=Msa0899900-mRNA-1:cds;Parent=Msa0899900-mRNA-1
Gene Sequence

>Msa0899900

ATGGGTAATGGCTACTCGGGAAATAATCCAATTGTTGAGCTATTCATTGGTAGCATAAGACTTGCTCGGGTGGCAACGATATCGCAACAATTAAAAACGTCGCCTCAGGTAACTTTGGGAAAACGTCCCCTTTGCCTAGACTTTAGGCGACGCTTAAAAATAGTGTGCCCAAAAGTGTGA

Protein sequence

>Msa0899900

MGNGYSGNNPIVELFIGSIRLARVATISQQLKTSPQVTLGKRPLCLDFRRRLKIVCPKV*