AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0962770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0962770 MtrunA17_Chr4g0071151 68.627 51 16 0 38 88 3 53 2.49e-14 65.5
Msa0962770 MtrunA17_Chr2g0328241 68.750 64 20 0 19 82 401 464 2.02e-12 60.8
Msa0962770 MtrunA17_Chr3g0111661 52.459 61 29 0 22 82 382 442 1.11e-11 58.9
Msa0962770 MtrunA17_Chr5g0435301 50.000 62 31 0 19 80 859 920 1.23e-11 58.9
Msa0962770 MtrunA17_Chr2g0328251 54.321 81 21 1 19 83 401 481 1.36e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0962770 AT4G01180.1 52.459 61 29 0 16 76 277 337 4.81e-12 60.5
Msa0962770 AT5G59390.2 53.448 58 27 0 19 76 285 342 1.01e-11 59.3
Msa0962770 AT5G59390.4 53.448 58 27 0 19 76 285 342 1.01e-11 59.3
Msa0962770 AT5G59390.3 53.448 58 27 0 19 76 285 342 1.01e-11 59.3
Msa0962770 AT1G13790.2 46.875 64 34 0 19 82 471 534 4.44e-11 57.8
Msa0962770 AT1G13790.1 46.875 64 34 0 19 82 471 534 4.44e-11 57.8

Find 30 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGAAATTATTTGTCTCTA+AGG 0.241880 6_3:+86257227 None:intergenic
TGGAGACTTATTTGTCTCTA+AGG 0.270769 6_3:-86257168 Msa0962770:intron
CCAAGTCTGCTAAATTTCTA+AGG 0.285019 6_3:+86257188 None:intergenic
TTCATATCTGCTTATATTAG+TGG 0.329683 6_3:-86253379 Msa0962770:three_prime_UTR
ACTTATTTATATTTAGTAAT+GGG 0.349767 6_3:-86257320 Msa0962770:three_prime_UTR
AACTAGAAATTTAGCAAACT+TGG 0.352418 6_3:-86253541 Msa0962770:three_prime_UTR
CCTTAGAAATTTAGCAGACT+TGG 0.364058 6_3:-86257188 Msa0962770:three_prime_UTR
ACTTGATGCAAGACATGCTT+TGG 0.365400 6_3:-86257457 Msa0962770:CDS
TGCAAGACATGCTTTGGAAT+TGG 0.380086 6_3:-86257451 Msa0962770:CDS
GACTTATTTATATTTAGTAA+TGG 0.441698 6_3:-86257321 Msa0962770:three_prime_UTR
TCACCAAGGGTTTCCTCAAA+AGG 0.453923 6_3:+86253340 None:intergenic
ATTGCATCACTTATCTCGTC+TGG 0.458810 6_3:+86257674 None:intergenic
TTGGATGTTGCAGAAAGAAA+AGG 0.471032 6_3:-86257517 Msa0962770:intron
TTATATTTAGTAATGGGTGT+TGG 0.513099 6_3:-86257314 Msa0962770:three_prime_UTR
TGCAATATCAGAGCAACAAA+AGG 0.514710 6_3:-86257657 Msa0962770:CDS
GAAGCTGCAGGAAAAGTAAG+AGG 0.515092 6_3:-86257370 Msa0962770:exon
GGGTGTTGGTGTGTCTGTAG+TGG 0.521988 6_3:-86257300 Msa0962770:three_prime_UTR
ACTAGAAATTTAGCAAACTT+GGG 0.523150 6_3:-86253540 Msa0962770:three_prime_UTR
GCGTTTGGCTGAAGACCATA+AGG 0.531394 6_3:-86257618 Msa0962770:intron
TACTAAATATAAATAAGTCG+TGG 0.532381 6_3:+86257325 None:intergenic
GGAAGTAGAAATGAAGCTGC+AGG 0.553371 6_3:-86257382 Msa0962770:CDS
ACTAAATATAAATAAGTCGT+GGG 0.563621 6_3:+86257326 None:intergenic
TTGATATCAACTTCTAGTGG+GGG 0.591702 6_3:+86253473 None:intergenic
GAGGAATTAATATAGCAAGT+AGG 0.597914 6_3:-86257351 Msa0962770:three_prime_UTR
ATCTGCTTATATTAGTGGTG+AGG 0.610762 6_3:-86253374 Msa0962770:three_prime_UTR
TTTGATATCAACTTCTAGTG+GGG 0.628815 6_3:+86253472 None:intergenic
TCACAAGAAAATGCATGACC+TGG 0.631525 6_3:-86257487 Msa0962770:CDS
TTAAGCTAATACCTCACCAA+GGG 0.631543 6_3:+86253327 None:intergenic
TTGGAGATAGAACAGCTGAA+AGG 0.635079 6_3:-86257432 Msa0962770:CDS
GTTAAGCTAATACCTCACCA+AGG 0.674046 6_3:+86253326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAAAAAATGTTGATAAA+AGG + chr6_3:86253437-86253456 None:intergenic 10.0%
!! ACTTATTTATATTTAGTAAT+GGG - chr6_3:86253694-86253713 Msa0962770:intron 10.0%
!!! ATTTGTTATTATGAATTTTA+AGG + chr6_3:86254294-86254313 None:intergenic 10.0%
!! GACTTATTTATATTTAGTAA+TGG - chr6_3:86253693-86253712 Msa0962770:intron 15.0%
!! TATACATTCTAATATTTAGA+AGG - chr6_3:86254535-86254554 Msa0962770:intron 15.0%
!! ACTAAATATAAATAAGTCGT+GGG + chr6_3:86253691-86253710 None:intergenic 20.0%
!! TACTAAATATAAATAAGTCG+TGG + chr6_3:86253692-86253711 None:intergenic 20.0%
!! ATAGAAATTATTTGTCTCTA+AGG + chr6_3:86253790-86253809 None:intergenic 20.0%
!! TGTATAATGTGATGATTAAT+GGG + chr6_3:86254521-86254540 None:intergenic 20.0%
!! ATGTATAATGTGATGATTAA+TGG + chr6_3:86254522-86254541 None:intergenic 20.0%
!! TATAGTGTATGTATACATAT+TGG - chr6_3:86254635-86254654 Msa0962770:intron 20.0%
!! ATAGTGTATGTATACATATT+GGG - chr6_3:86254636-86254655 Msa0962770:intron 20.0%
!! ACTTACAATTCTTTATTAGA+TGG + chr6_3:86254732-86254751 None:intergenic 20.0%
!!! CATTTCTTTTAAGAGATTTA+TGG - chr6_3:86254887-86254906 Msa0962770:intron 20.0%
!! ATAACAGTATCATGATTAAT+GGG + chr6_3:86254941-86254960 None:intergenic 20.0%
!! AATAACAGTATCATGATTAA+TGG + chr6_3:86254942-86254961 None:intergenic 20.0%
!! AAAATAAAACTGCATTCTTA+TGG + chr6_3:86255116-86255135 None:intergenic 20.0%
!!! AATAAACTTGTTTTCATCAA+TGG - chr6_3:86255517-86255536 Msa0962770:intron 20.0%
!!! ATAGTAACAATGGTTTTATT+TGG + chr6_3:86256618-86256637 None:intergenic 20.0%
!! ACATTAGAAATCTTAAAATG+TGG + chr6_3:86256669-86256688 None:intergenic 20.0%
!! ATAGAAATTATTTGTCTCTA+AGG + chr6_3:86253790-86253809 None:intergenic 20.0%
! TAATGAAAAAATGATGCGTT+TGG - chr6_3:86253381-86253400 Msa0962770:three_prime_UTR 25.0%
!!! TTATGTTTTGATGACTCTTT+TGG - chr6_3:86253474-86253493 Msa0962770:three_prime_UTR 25.0%
!!! TTATATTTAGTAATGGGTGT+TGG - chr6_3:86253700-86253719 Msa0962770:intron 25.0%
! AACTAGAAATTTAGCAAACT+TGG - chr6_3:86253891-86253910 Msa0962770:intron 25.0%
! ACTAGAAATTTAGCAAACTT+GGG - chr6_3:86253892-86253911 Msa0962770:intron 25.0%
!! TTTTGATATCAACTTCTAGT+GGG + chr6_3:86253964-86253983 None:intergenic 25.0%
! TTCATATCTGCTTATATTAG+TGG - chr6_3:86254053-86254072 Msa0962770:intron 25.0%
!! GGTATATATTTTTCCTTTTG+AGG - chr6_3:86254079-86254098 Msa0962770:intron 25.0%
! ACATTCTAATATTTAGAAGG+TGG - chr6_3:86254538-86254557 Msa0962770:intron 25.0%
! TATCAGGATCTTCTATTTAA+GGG + chr6_3:86254564-86254583 None:intergenic 25.0%
! AGAAATGCATTCGAAAAATA+TGG + chr6_3:86254874-86254893 None:intergenic 25.0%
! GTTATGTAAGAATAAAATGG+TGG + chr6_3:86254919-86254938 None:intergenic 25.0%
! TGGGTTATGTAAGAATAAAA+TGG + chr6_3:86254922-86254941 None:intergenic 25.0%
!! AGTGATACAAAGGTATATAT+AGG + chr6_3:86255087-86255106 None:intergenic 25.0%
! TCTTCCATTGTTTCATAATA+TGG - chr6_3:86255138-86255157 Msa0962770:intron 25.0%
! GATACCATATTATGAAACAA+TGG + chr6_3:86255145-86255164 None:intergenic 25.0%
!! TCTATCAACAATTTGAAACA+AGG - chr6_3:86255745-86255764 Msa0962770:intron 25.0%
! TCACTAAAATGAAAACTCTA+TGG - chr6_3:86255791-86255810 Msa0962770:intron 25.0%
! CACTAAAATGAAAACTCTAT+GGG - chr6_3:86255792-86255811 Msa0962770:intron 25.0%
! AATTAACAAGGTGTATTCAT+TGG - chr6_3:86255996-86256015 Msa0962770:intron 25.0%
! TATCTGATTCATCTAATGAT+TGG - chr6_3:86256502-86256521 Msa0962770:intron 25.0%
! TGAAGTAAAATAGTGTAAAG+AGG + chr6_3:86256562-86256581 None:intergenic 25.0%
! ATCAAATTAACCTTGAAATG+TGG + chr6_3:86256702-86256721 None:intergenic 25.0%
! TTTATGGATATATCACAATG+AGG + chr6_3:86256903-86256922 None:intergenic 25.0%
! GTAAAAGAACTAGAAACAAT+GGG + chr6_3:86256939-86256958 None:intergenic 25.0%
! AGTAAAAGAACTAGAAACAA+TGG + chr6_3:86256940-86256959 None:intergenic 25.0%
! CATATTTGACAATCTTTGAT+TGG + chr6_3:86257009-86257028 None:intergenic 25.0%
! TTATTATCAACACAGATTGA+GGG + chr6_3:86257044-86257063 None:intergenic 25.0%
! TTTATTATCAACACAGATTG+AGG + chr6_3:86257045-86257064 None:intergenic 25.0%
! AAATTGACAAACATCACAAA+TGG + chr6_3:86257069-86257088 None:intergenic 25.0%
!! AACTGATTCATTGTGATATT+TGG - chr6_3:86257147-86257166 Msa0962770:intron 25.0%
!! ACTGATTCATTGTGATATTT+GGG - chr6_3:86257148-86257167 Msa0962770:intron 25.0%
! AACAAAAATCATTGAAACAC+AGG + chr6_3:86257343-86257362 None:intergenic 25.0%
! AACTAGAAATTTAGCAAACT+TGG - chr6_3:86253891-86253910 Msa0962770:intron 25.0%
! ACTAGAAATTTAGCAAACTT+GGG - chr6_3:86253892-86253911 Msa0962770:intron 25.0%
!! TTTTGATATCAACTTCTAGT+GGG + chr6_3:86253964-86253983 None:intergenic 25.0%
! TTCATATCTGCTTATATTAG+TGG - chr6_3:86254053-86254072 Msa0962770:intron 25.0%
!! GGTATATATTTTTCCTTTTG+AGG - chr6_3:86254079-86254098 Msa0962770:intron 25.0%
AAAATCAAATGTGTGCCTTA+TGG + chr6_3:86253414-86253433 None:intergenic 30.0%
AAGAGTCATCAAAACATAAG+TGG + chr6_3:86253473-86253492 None:intergenic 30.0%
TTTCCGTGTAATGAAAAAAC+TGG - chr6_3:86253608-86253627 Msa0962770:intron 30.0%
GAGGAATTAATATAGCAAGT+AGG - chr6_3:86253663-86253682 Msa0962770:intron 30.0%
TTTGATATCAACTTCTAGTG+GGG + chr6_3:86253963-86253982 None:intergenic 30.0%
! CTTTTGATATCAACTTCTAG+TGG + chr6_3:86253965-86253984 None:intergenic 30.0%
ATTGTCCTTACTCTCAAAAA+TGG + chr6_3:86254469-86254488 None:intergenic 30.0%
!! TTAATGGGCTTTGTAAAGAA+GGG + chr6_3:86254506-86254525 None:intergenic 30.0%
!! ATTAATGGGCTTTGTAAAGA+AGG + chr6_3:86254507-86254526 None:intergenic 30.0%
CTATCAGGATCTTCTATTTA+AGG + chr6_3:86254565-86254584 None:intergenic 30.0%
ATGCAAAAATGGAAGACTTA+AGG + chr6_3:86254601-86254620 None:intergenic 30.0%
! ACATACACTATACTTGTTGA+TGG + chr6_3:86254627-86254646 None:intergenic 30.0%
TTGAAAAAGATGAAAGACGA+AGG + chr6_3:86254669-86254688 None:intergenic 30.0%
ATTTGCAAAGTCCATCAATA+AGG - chr6_3:86254823-86254842 Msa0962770:intron 30.0%
TTTGCAAAGTCCATCAATAA+GGG - chr6_3:86254824-86254843 Msa0962770:intron 30.0%
ACATACAATTCCCTTATTGA+TGG + chr6_3:86254837-86254856 None:intergenic 30.0%
ACTAAGATGATGAAGTACAA+TGG + chr6_3:86255028-86255047 None:intergenic 30.0%
GATTGTGTAGTACAATTCTT+AGG - chr6_3:86255061-86255080 Msa0962770:intron 30.0%
ATGGAAATAGAGTGATACAA+AGG + chr6_3:86255097-86255116 None:intergenic 30.0%
AAATGAGAAGAAAAACGGTT+AGG + chr6_3:86255218-86255237 None:intergenic 30.0%
GGAAGAAATGAGAAGAAAAA+CGG + chr6_3:86255223-86255242 None:intergenic 30.0%
! CAAAAGTACAGAAAAAACAG+AGG + chr6_3:86255325-86255344 None:intergenic 30.0%
! AAGCTTTTCATCTTCAACAA+TGG - chr6_3:86255345-86255364 Msa0962770:intron 30.0%
AAATCATCAAGATCAGGAAT+AGG + chr6_3:86255550-86255569 None:intergenic 30.0%
ACTAAAGTTTGAGCAATTTG+TGG + chr6_3:86255637-86255656 None:intergenic 30.0%
! CAAGTGATTCAATTTTTGAC+TGG - chr6_3:86255913-86255932 Msa0962770:intron 30.0%
TCCATTACCTTCAATTAACA+AGG - chr6_3:86255984-86256003 Msa0962770:intron 30.0%
ACCTTGTTAATTGAAGGTAA+TGG + chr6_3:86255988-86256007 None:intergenic 30.0%
GAATACACCTTGTTAATTGA+AGG + chr6_3:86255994-86256013 None:intergenic 30.0%
! GTATTCATTGGTTATACAAG+AGG - chr6_3:86256008-86256027 Msa0962770:intron 30.0%
ACAAAACCTAGAGTTATTCT+TGG + chr6_3:86256145-86256164 None:intergenic 30.0%
AGGATTGGATTTGTATGAAT+CGG + chr6_3:86256226-86256245 None:intergenic 30.0%
! AAGCAATATGGTTTGAAGTT+GGG + chr6_3:86256413-86256432 None:intergenic 30.0%
! AAAACAGTTGATATACCTGA+AGG + chr6_3:86256471-86256490 None:intergenic 30.0%
! AATGATTGGTTACTTGATTC+TGG - chr6_3:86256516-86256535 Msa0962770:intron 30.0%
TTTGGTAAATGAACATGAAC+AGG + chr6_3:86256600-86256619 None:intergenic 30.0%
AGCATGATGTATAGTAACAA+TGG + chr6_3:86256628-86256647 None:intergenic 30.0%
ATTGTTACTATACATCATGC+TGG - chr6_3:86256627-86256646 Msa0962770:intron 30.0%
CTTATATTCACCACATTTCA+AGG - chr6_3:86256689-86256708 Msa0962770:intron 30.0%
! ATTCAAGCTGCTAATGATTT+TGG + chr6_3:86256733-86256752 None:intergenic 30.0%
ATTTGTATAAACCACCATGA+GGG + chr6_3:86256830-86256849 None:intergenic 30.0%
AATTTGTATAAACCACCATG+AGG + chr6_3:86256831-86256850 None:intergenic 30.0%
! GGGTTGAACATCTTTATTTA+TGG + chr6_3:86256919-86256938 None:intergenic 30.0%
TGAGATAAACTATGAGGAAA+AGG + chr6_3:86257110-86257129 None:intergenic 30.0%
CTGATTCATTGTGATATTTG+GGG - chr6_3:86257149-86257168 Msa0962770:intron 30.0%
TACTTATGACCATGAATAGA+AGG + chr6_3:86257188-86257207 None:intergenic 30.0%
!! CTATTGTTGATGACTTTAGT+AGG - chr6_3:86257216-86257235 Msa0962770:three_prime_UTR 30.0%
! ATGACTTTAGTAGGTTTGTT+TGG - chr6_3:86257225-86257244 Msa0962770:three_prime_UTR 30.0%
! TCATTGAAACACAGGATTTT+AGG + chr6_3:86257335-86257354 None:intergenic 30.0%
TTTGATATCAACTTCTAGTG+GGG + chr6_3:86253963-86253982 None:intergenic 30.0%
! CTTTTGATATCAACTTCTAG+TGG + chr6_3:86253965-86253984 None:intergenic 30.0%
TGCAATATCAGAGCAACAAA+AGG - chr6_3:86253357-86253376 Msa0962770:three_prime_UTR 35.0%
!! GTTTTGATGACTCTTTTGGT+TGG - chr6_3:86253478-86253497 Msa0962770:three_prime_UTR 35.0%
TTGGATGTTGCAGAAAGAAA+AGG - chr6_3:86253497-86253516 Msa0962770:three_prime_UTR 35.0%
!!! CCTCCAGTTTTTTCATTACA+CGG + chr6_3:86253614-86253633 None:intergenic 35.0%
CCAAGTCTGCTAAATTTCTA+AGG + chr6_3:86253829-86253848 None:intergenic 35.0%
CCTTAGAAATTTAGCAGACT+TGG - chr6_3:86253826-86253845 Msa0962770:intron 35.0%
TGGAGACTTATTTGTCTCTA+AGG - chr6_3:86253846-86253865 Msa0962770:intron 35.0%
TTGATATCAACTTCTAGTGG+GGG + chr6_3:86253962-86253981 None:intergenic 35.0%
ATCTGCTTATATTAGTGGTG+AGG - chr6_3:86254058-86254077 Msa0962770:intron 35.0%
TTAAGCTAATACCTCACCAA+GGG + chr6_3:86254108-86254127 None:intergenic 35.0%
CTTCATGAAATGATTGCTAG+AGG + chr6_3:86254351-86254370 None:intergenic 35.0%
TGAAAGCGGCAAAAATGATA+AGG + chr6_3:86254388-86254407 None:intergenic 35.0%
!! ATTCGTGCTTTGTTTGAAAG+CGG + chr6_3:86254402-86254421 None:intergenic 35.0%
!! GTCTTCCATTTTTGAGAGTA+AGG - chr6_3:86254461-86254480 Msa0962770:intron 35.0%
!! GTTGATGGACTATGCAAAAA+TGG + chr6_3:86254612-86254631 None:intergenic 35.0%
TTGTAAGTGATTATGTCAGC+TGG - chr6_3:86254744-86254763 Msa0962770:intron 35.0%
ATAGATGAGATGCATGATAG+AGG + chr6_3:86254774-86254793 None:intergenic 35.0%
AGGGAGAATATCTGATGTTT+GGG + chr6_3:86254802-86254821 None:intergenic 35.0%
!! TTGATGGACTTTGCAAATCA+GGG + chr6_3:86254821-86254840 None:intergenic 35.0%
!! ATTGATGGACTTTGCAAATC+AGG + chr6_3:86254822-86254841 None:intergenic 35.0%
GAATTGTATGTTACAGTGTC+AGG - chr6_3:86254846-86254865 Msa0962770:intron 35.0%
GAAGAACACGAAGAAAACAA+TGG + chr6_3:86255296-86255315 None:intergenic 35.0%
TTCTATGTTCATCCTAGTGA+GGG - chr6_3:86255406-86255425 Msa0962770:intron 35.0%
!! AAGGTAATGGATCCAAAAGA+AGG + chr6_3:86255975-86255994 None:intergenic 35.0%
ATACAAGAGGAGAGTAACAA+TGG - chr6_3:86256021-86256040 Msa0962770:intron 35.0%
! TGAAGCATTAACAAGAATGC+TGG + chr6_3:86256079-86256098 None:intergenic 35.0%
! TCAGACTCTAAGAAGCAATA+TGG - chr6_3:86256096-86256115 Msa0962770:intron 35.0%
TCTAAGAAGCAATATGGCAA+TGG - chr6_3:86256102-86256121 Msa0962770:intron 35.0%
!!! GAGTTTTGTTATCAGAAGCA+TGG - chr6_3:86256195-86256214 Msa0962770:intron 35.0%
GATTGGATTTGTATGAATCG+GGG + chr6_3:86256224-86256243 None:intergenic 35.0%
GGATTGGATTTGTATGAATC+GGG + chr6_3:86256225-86256244 None:intergenic 35.0%
CAATCACATTTGTTTGCTTC+AGG - chr6_3:86256372-86256391 Msa0962770:intron 35.0%
! AGCAATATGGTTTGAAGTTG+GGG + chr6_3:86256412-86256431 None:intergenic 35.0%
! GAAGCAATATGGTTTGAAGT+TGG + chr6_3:86256414-86256433 None:intergenic 35.0%
AATTAGGAGCAGAAGCAATA+TGG + chr6_3:86256425-86256444 None:intergenic 35.0%
CATGAACAGGTTTGATAGAT+TGG + chr6_3:86256587-86256606 None:intergenic 35.0%
ACCTGATAAATGCATCATAC+AGG - chr6_3:86256770-86256789 Msa0962770:intron 35.0%
TCCTGTATGATGCATTTATC+AGG + chr6_3:86256774-86256793 None:intergenic 35.0%
GACTGTAAAGGAAAAGCTAA+AGG + chr6_3:86256864-86256883 None:intergenic 35.0%
TGAGAAACAGAAGACTGTAA+AGG + chr6_3:86256876-86256895 None:intergenic 35.0%
ATATTCCTGAAACTGCACTT+TGG - chr6_3:86256961-86256980 Msa0962770:intron 35.0%
GTAGCATGAGATAAACTATG+AGG + chr6_3:86257116-86257135 None:intergenic 35.0%
TGAATAGAAGGAACACTCAA+AGG + chr6_3:86257176-86257195 None:intergenic 35.0%
TTGAGTGTTCCTTCTATTCA+TGG - chr6_3:86257176-86257195 Msa0962770:three_prime_UTR 35.0%
TTTGATCAAATCCAAAGCTG+AGG - chr6_3:86257253-86257272 Msa0962770:three_prime_UTR 35.0%
CCAAGTCTGCTAAATTTCTA+AGG + chr6_3:86253829-86253848 None:intergenic 35.0%
CCTTAGAAATTTAGCAGACT+TGG - chr6_3:86253826-86253845 Msa0962770:intron 35.0%
TGGAGACTTATTTGTCTCTA+AGG - chr6_3:86253846-86253865 Msa0962770:intron 35.0%
TTGATATCAACTTCTAGTGG+GGG + chr6_3:86253962-86253981 None:intergenic 35.0%
ATCTGCTTATATTAGTGGTG+AGG - chr6_3:86254058-86254077 Msa0962770:intron 35.0%
TTAAGCTAATACCTCACCAA+GGG + chr6_3:86254108-86254127 None:intergenic 35.0%
ATTGCATCACTTATCTCGTC+TGG + chr6_3:86253343-86253362 None:intergenic 40.0%
TCACAAGAAAATGCATGACC+TGG - chr6_3:86253527-86253546 Msa0962770:three_prime_UTR 40.0%
! TTGCATCAAGTCTTTTCACC+AGG + chr6_3:86253548-86253567 None:intergenic 40.0%
ACTTGATGCAAGACATGCTT+TGG - chr6_3:86253557-86253576 Msa0962770:intron 40.0%
!! TGCAAGACATGCTTTGGAAT+TGG - chr6_3:86253563-86253582 Msa0962770:intron 40.0%
TTGGAGATAGAACAGCTGAA+AGG - chr6_3:86253582-86253601 Msa0962770:intron 40.0%
CCGTGTAATGAAAAAACTGG+AGG - chr6_3:86253611-86253630 Msa0962770:intron 40.0%
!! TTTCCTTTTGAGGAAACCCT+TGG - chr6_3:86254089-86254108 Msa0962770:intron 40.0%
GTTAAGCTAATACCTCACCA+AGG + chr6_3:86254109-86254128 None:intergenic 40.0%
TGCTAGAGGTTTACTCTAAG+AGG + chr6_3:86254337-86254356 None:intergenic 40.0%
AAGCGGCAAAAATGATAAGG+CGG + chr6_3:86254385-86254404 None:intergenic 40.0%
GTTTCGTAAGTTACTGCATC+AGG - chr6_3:86254426-86254445 Msa0962770:intron 40.0%
CTCTCAAAAATGGAAGACAG+CGG + chr6_3:86254459-86254478 None:intergenic 40.0%
AAGACTTAAGGATGCAGCAA+GGG + chr6_3:86254589-86254608 None:intergenic 40.0%
!! CAAAAACCATCAGGTTGACA+GGG + chr6_3:86254703-86254722 None:intergenic 40.0%
TGGATTGTGCAAAAACCATC+AGG + chr6_3:86254712-86254731 None:intergenic 40.0%
CAGGGAGAATATCTGATGTT+TGG + chr6_3:86254803-86254822 None:intergenic 40.0%
GGTTAGGATCGCAAGAAAAA+CGG + chr6_3:86255202-86255221 None:intergenic 40.0%
GTTCTATGTTCATCCTAGTG+AGG - chr6_3:86255405-86255424 Msa0962770:intron 40.0%
GGAGTAACTACCAAAGAGTT+TGG + chr6_3:86255433-86255452 None:intergenic 40.0%
CGGTTCAAATCATCAAGATC+AGG + chr6_3:86255556-86255575 None:intergenic 40.0%
ATGATTTGAACCGTGATGCA+TGG - chr6_3:86255563-86255582 Msa0962770:intron 40.0%
TGATTTGAACCGTGATGCAT+GGG - chr6_3:86255564-86255583 Msa0962770:intron 40.0%
! GCAATCATCTGGTTTATTCG+TGG - chr6_3:86255593-86255612 Msa0962770:intron 40.0%
ACTCAAAGAACGCTTCTCAA+AGG - chr6_3:86255690-86255709 Msa0962770:intron 40.0%
GGAAATGGACAAGTGCAATT+AGG + chr6_3:86255844-86255863 None:intergenic 40.0%
CAAATCTCATAGCTTCACAC+CGG + chr6_3:86255870-86255889 None:intergenic 40.0%
! CAAGTCTCAAGTCCTTCTTT+TGG - chr6_3:86255960-86255979 Msa0962770:intron 40.0%
!! CTGGAATCTTCTGAAACAGA+GGG + chr6_3:86256060-86256079 None:intergenic 40.0%
CTGGTTCCAAGAATAACTCT+AGG - chr6_3:86256136-86256155 Msa0962770:intron 40.0%
CAAACATACAAACCACACTG+TGG - chr6_3:86256173-86256192 Msa0962770:intron 40.0%
GATAACAAAACTCCACAGTG+TGG + chr6_3:86256188-86256207 None:intergenic 40.0%
!! AGTAGCATTTGCTGTAGGAT+TGG + chr6_3:86256241-86256260 None:intergenic 40.0%
!! ACAGTAGTAGCATTTGCTGT+AGG + chr6_3:86256246-86256265 None:intergenic 40.0%
! GCAATATGGTTTGAAGTTGG+GGG + chr6_3:86256411-86256430 None:intergenic 40.0%
! ACAGTTGATATACCTGAAGG+AGG + chr6_3:86256468-86256487 None:intergenic 40.0%
TGCACTTTGGCACTTTAGAT+TGG - chr6_3:86256974-86256993 Msa0962770:intron 40.0%
GCACTTTGGCACTTTAGATT+GGG - chr6_3:86256975-86256994 Msa0962770:intron 40.0%
CACTTTGGCACTTTAGATTG+GGG - chr6_3:86256976-86256995 Msa0962770:intron 40.0%
ATGAATAGACACCTCAGCTT+TGG + chr6_3:86257267-86257286 None:intergenic 40.0%
!! TTTCCTTTTGAGGAAACCCT+TGG - chr6_3:86254089-86254108 Msa0962770:intron 40.0%
GTTAAGCTAATACCTCACCA+AGG + chr6_3:86254109-86254128 None:intergenic 40.0%
GGAAGTAGAAATGAAGCTGC+AGG - chr6_3:86253632-86253651 Msa0962770:intron 45.0%
GAAGCTGCAGGAAAAGTAAG+AGG - chr6_3:86253644-86253663 Msa0962770:intron 45.0%
TCACCAAGGGTTTCCTCAAA+AGG + chr6_3:86254095-86254114 None:intergenic 45.0%
!! TTTTGAGGAAACCCTTGGTG+AGG - chr6_3:86254094-86254113 Msa0962770:intron 45.0%
GAAGACTTAAGGATGCAGCA+AGG + chr6_3:86254590-86254609 None:intergenic 45.0%
!! GCAAAAACCATCAGGTTGAC+AGG + chr6_3:86254704-86254723 None:intergenic 45.0%
ATTACAGCAGCATGCAGAGA+AGG - chr6_3:86254959-86254978 Msa0962770:intron 45.0%
GGATCGCAAGAAAAACGGTT+AGG + chr6_3:86255197-86255216 None:intergenic 45.0%
CCAGAGAAGATGAATTGCAG+CGG + chr6_3:86255244-86255263 None:intergenic 45.0%
AAAGAGTTTGGACCCTCACT+AGG + chr6_3:86255421-86255440 None:intergenic 45.0%
TAGTGAGGGTCCAAACTCTT+TGG - chr6_3:86255420-86255439 Msa0962770:intron 45.0%
GTAGTTACTCCACTTCTCAC+TGG - chr6_3:86255442-86255461 Msa0962770:intron 45.0%
TAATTCGAGCCAGTGAGAAG+TGG + chr6_3:86255454-86255473 None:intergenic 45.0%
GAGTTCATCCCAAACGTCAA+TGG + chr6_3:86255674-86255693 None:intergenic 45.0%
GGACAAGTGCAATTAGGCAT+TGG + chr6_3:86255838-86255857 None:intergenic 45.0%
!! GCTGGAATCTTCTGAAACAG+AGG + chr6_3:86256061-86256080 None:intergenic 45.0%
! GGCAATGGAAAGTCTTTTGC+TGG - chr6_3:86256117-86256136 Msa0962770:intron 45.0%
TTGTCAACTGCAGAGTTCAC+AGG + chr6_3:86256294-86256313 None:intergenic 45.0%
GTTGACAATTCTCCTCCTAC+TGG - chr6_3:86256306-86256325 Msa0962770:intron 45.0%
CCAGAGGAAGAAGGGAAATT+AGG + chr6_3:86256441-86256460 None:intergenic 45.0%
CCTAATTTCCCTTCTTCCTC+TGG - chr6_3:86256438-86256457 Msa0962770:intron 45.0%
! TACCCACAGGATGATTGGTT+TGG - chr6_3:86256800-86256819 Msa0962770:intron 45.0%
AGCCAAACCAATCATCCTGT+GGG + chr6_3:86256805-86256824 None:intergenic 45.0%
AAGTGCCAAAGTGCAGTTTC+AGG + chr6_3:86256969-86256988 None:intergenic 45.0%
TCACCAAGGGTTTCCTCAAA+AGG + chr6_3:86254095-86254114 None:intergenic 45.0%
!! TTTTGAGGAAACCCTTGGTG+AGG - chr6_3:86254094-86254113 Msa0962770:intron 45.0%
GCGTTTGGCTGAAGACCATA+AGG - chr6_3:86253396-86253415 Msa0962770:three_prime_UTR 50.0%
CAATTGCCCTGTCAACCTGA+TGG - chr6_3:86254694-86254713 Msa0962770:intron 50.0%
CCGCTGCAATTCATCTTCTC+TGG - chr6_3:86255241-86255260 Msa0962770:intron 50.0%
! CTGGCTCGAATTATCTCGCA+TGG - chr6_3:86255461-86255480 Msa0962770:intron 50.0%
AGATCCATGCAACGTGCACT+TGG - chr6_3:86255487-86255506 Msa0962770:intron 50.0%
TGAACCGTGATGCATGGGAA+CGG - chr6_3:86255569-86255588 Msa0962770:intron 50.0%
ATGGGAACGGTGCAATCATC+TGG - chr6_3:86255582-86255601 Msa0962770:intron 50.0%
ATGAGCACGCCATTGACGTT+TGG - chr6_3:86255662-86255681 Msa0962770:intron 50.0%
TGAGCACGCCATTGACGTTT+GGG - chr6_3:86255663-86255682 Msa0962770:intron 50.0%
GCACTTGTCCATTTCCATGC+CGG - chr6_3:86255848-86255867 Msa0962770:intron 50.0%
ATTTGCACGCGCACATCGTA+TGG - chr6_3:86255885-86255904 Msa0962770:intron 50.0%
!! TCCTCCTACTGGTTTGACTC+AGG - chr6_3:86256317-86256336 Msa0962770:intron 50.0%
TCCTGAGTCAAACCAGTAGG+AGG + chr6_3:86256321-86256340 None:intergenic 50.0%
TGCTCCTGAGTCAAACCAGT+AGG + chr6_3:86256324-86256343 None:intergenic 50.0%
! TGGTTTGAAGTTGGGGGAGA+AGG + chr6_3:86256405-86256424 None:intergenic 50.0%
TACAGGATCTGCTTACCCAC+AGG - chr6_3:86256787-86256806 Msa0962770:intron 50.0%
CTGCTTACCCACAGGATGAT+TGG - chr6_3:86256795-86256814 Msa0962770:intron 50.0%
CAGCCAAACCAATCATCCTG+TGG + chr6_3:86256806-86256825 None:intergenic 50.0%
!! ATTGGTTTGGCTGACCCTCA+TGG - chr6_3:86256813-86256832 Msa0962770:intron 50.0%
!! GGGTGTTGGTGTGTCTGTAG+TGG - chr6_3:86253714-86253733 Msa0962770:intron 55.0%
GGATGCAGCAAGGGTCTATC+AGG + chr6_3:86254580-86254599 None:intergenic 55.0%
TGCACCGTTCCCATGCATCA+CGG + chr6_3:86255576-86255595 None:intergenic 55.0%
GCTTCACACCGGCATGGAAA+TGG + chr6_3:86255859-86255878 None:intergenic 55.0%
CTCATAGCTTCACACCGGCA+TGG + chr6_3:86255865-86255884 None:intergenic 55.0%
TGAAGTTGGGGGAGAAGGAG+TGG + chr6_3:86256400-86256419 None:intergenic 55.0%
!! GAGGAGTGCCAGAGGAAGAA+GGG + chr6_3:86256449-86256468 None:intergenic 55.0%
TGCACGCGCACATCGTATGG+AGG - chr6_3:86255888-86255907 Msa0962770:intron 60.0%
!! GGAGGAGTGCCAGAGGAAGA+AGG + chr6_3:86256450-86256469 None:intergenic 60.0%
TCCTCTGGCACTCCTCCTTC+AGG - chr6_3:86256453-86256472 Msa0962770:intron 60.0%
!! ACCTGAAGGAGGAGTGCCAG+AGG + chr6_3:86256457-86256476 None:intergenic 60.0%
GGTTTGGCTGACCCTCATGG+TGG - chr6_3:86256816-86256835 Msa0962770:intron 60.0%
CGCGCCAAGTGCACGTTGCA+TGG + chr6_3:86255494-86255513 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 86253304 86257732 86253304 ID=Msa0962770;Name=Msa0962770
chr6_3 mRNA 86253304 86257732 86253304 ID=Msa0962770-mRNA-1;Parent=Msa0962770;Name=Msa0962770-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0.5|0.66|0.66|0.5|0.33|3|474|88
chr6_3 exon 86253304 86253572 86253304 ID=Msa0962770-mRNA-1:exon:15587;Parent=Msa0962770-mRNA-1
chr6_3 exon 86257169 86257526 86257169 ID=Msa0962770-mRNA-1:exon:15586;Parent=Msa0962770-mRNA-1
chr6_3 exon 86257619 86257732 86257619 ID=Msa0962770-mRNA-1:exon:15585;Parent=Msa0962770-mRNA-1
chr6_3 CDS 86257619 86257732 86257619 ID=Msa0962770-mRNA-1:cds;Parent=Msa0962770-mRNA-1
chr6_3 CDS 86257374 86257526 86257374 ID=Msa0962770-mRNA-1:cds;Parent=Msa0962770-mRNA-1
chr6_3 three_prime_UTR 86257169 86257373 86257169 ID=Msa0962770-mRNA-1:three_prime_utr;Parent=Msa0962770-mRNA-1
chr6_3 three_prime_UTR 86253304 86253572 86253304 ID=Msa0962770-mRNA-1:three_prime_utr;Parent=Msa0962770-mRNA-1
Gene Sequence

>Msa0962770

ATGGCGGAAGCTGGGGTGGATAACATCAGCAGTTTACCAGACGAGATAAGTGATGCAATATCAGAGCAACAAAAGGCTAATGAAAAAATGATGCGTTTGGCTGAAGACCATAAGAAAGAAAAGGAAAAATTTCACAAGAAAATGCATGACCTGGTGAAAAGACTTGATGCAAGACATGCTTTGGAATTGGAGATAGAACAGCTGAAAGGAGTTTTCCGTGTAATGAAAAAACTGGAGGAAGTAGAAATGAAGCTGCAGGAAAAGTAA
Protein sequence

>Msa0962770

MAEAGVDNISSLPDEISDAISEQQKANEKMMRLAEDHKKEKEKFHKKMHDLVKRLDARHALELEIEQLKGVFRVMKKLEEVEMKLQEK*