Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0999320 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006980 | Msa0999320 | 0.808274 | 3.356109e-50 | -8.615850e-47 |
| Msa0055450 | Msa0999320 | 0.856188 | 3.808143e-62 | -8.615850e-47 |
| Msa0669840 | Msa0999320 | 0.813486 | 2.498364e-51 | -8.615850e-47 |
| Msa0725860 | Msa0999320 | 0.807018 | 6.202914e-50 | -8.615850e-47 |
| Msa0739580 | Msa0999320 | 0.842014 | 3.348535e-58 | -8.615850e-47 |
| Msa0782360 | Msa0999320 | 0.815670 | 8.207574e-52 | -8.615850e-47 |
| Msa0819890 | Msa0999320 | 0.806082 | 9.776827e-50 | -8.615850e-47 |
| Msa0823400 | Msa0999320 | 0.801951 | 7.066828e-49 | -8.615850e-47 |
| Msa0124130 | Msa0999320 | 0.860496 | 1.984099e-63 | -8.615850e-47 |
| Msa0141120 | Msa0999320 | 0.826219 | 3.058052e-54 | -8.615850e-47 |
| Msa0149140 | Msa0999320 | 0.823183 | 1.588884e-53 | -8.615850e-47 |
| Msa0212150 | Msa0999320 | 0.806152 | 9.448210e-50 | -8.615850e-47 |
| Msa0406000 | Msa0999320 | 0.840541 | 8.172974e-58 | -8.615850e-47 |
| Msa0424340 | Msa0999320 | 0.828188 | 1.032765e-54 | -8.615850e-47 |
| Msa0424350 | Msa0999320 | 0.829987 | 3.783736e-55 | -8.615850e-47 |
| Msa0451400 | Msa0999320 | 0.841485 | 4.618880e-58 | -8.615850e-47 |
| Msa0999300 | Msa0999320 | 0.819063 | 1.413868e-52 | -8.615850e-47 |
| Msa0999320 | Msa0999330 | 0.802724 | 4.897353e-49 | -8.615850e-47 |
| Msa0999320 | Msa0999350 | 0.821281 | 4.389846e-53 | -8.615850e-47 |
| Msa0999320 | Msa0999380 | 0.817124 | 3.880940e-52 | -8.615850e-47 |
| Msa0999320 | Msa0999400 | 0.812929 | 3.310570e-51 | -8.615850e-47 |
| Msa0999320 | Msa1001870 | 0.999616 | 0.000000e+00 | -8.615850e-47 |
| Msa0999320 | Msa1201160 | 0.817503 | 3.188017e-52 | -8.615850e-47 |
| Msa0999320 | Msa1228190 | 0.805800 | 1.120827e-49 | -8.615850e-47 |
| Msa0999320 | Msa1271260 | 0.805885 | 1.075725e-49 | -8.615850e-47 |
| Msa0999320 | Msa1294290 | 0.820992 | 5.116742e-53 | -8.615850e-47 |
| Msa0999320 | Msa1326630 | 0.816660 | 4.930921e-52 | -8.615850e-47 |
| Msa0999320 | Msa1379970 | 0.808174 | 3.525908e-50 | -8.615850e-47 |
| Msa0999320 | Msa1406780 | 0.836493 | 9.061927e-57 | -8.615850e-47 |
| Msa0999320 | Msa1431850 | 0.845859 | 3.121678e-59 | -8.615850e-47 |
| Msa0252770 | Msa0999320 | 0.813826 | 2.103035e-51 | -8.615850e-47 |
| Msa0306120 | Msa0999320 | 0.816598 | 5.091936e-52 | -8.615850e-47 |
| Msa0329620 | Msa0999320 | 0.812999 | 3.196275e-51 | -8.615850e-47 |
| Msa0343660 | Msa0999320 | 0.809347 | 1.978795e-50 | -8.615850e-47 |
| Msa0359250 | Msa0999320 | 0.828533 | 8.529872e-55 | -8.615850e-47 |
| Msa0492190 | Msa0999320 | 0.826828 | 2.189269e-54 | -8.615850e-47 |
| Msa0515950 | Msa0999320 | 0.817990 | 2.475821e-52 | -8.615850e-47 |
| Msa0591790 | Msa0999320 | 0.811532 | 6.680260e-51 | -8.615850e-47 |
| Msa0591800 | Msa0999320 | 0.812229 | 4.708848e-51 | -8.615850e-47 |
| Msa0860280 | Msa0999320 | 0.827851 | 1.244891e-54 | -8.615850e-47 |
| Msa0898730 | Msa0999320 | 0.808497 | 3.008924e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTTAAATTATCTTCATTT+TGG | 0.128759 | 6_4:+81256118 | Msa0999320:exon |
| GCCTGCTGCACAAACTTTGC+TGG | 0.372514 | 6_4:-81255846 | None:intergenic |
| TTGTCCAATTGCACAGCAAA+AGG | 0.389238 | 6_4:-81256198 | None:intergenic |
| CCCCACACCATGCGCCTACT+TGG | 0.394115 | 6_4:+81255959 | Msa0999320:CDS |
| ACCAAGTAGGCGCATGGTGT+GGG | 0.413182 | 6_4:-81255960 | None:intergenic |
| CCCAGCAAAGTTTGTGCAGC+AGG | 0.418179 | 6_4:+81255845 | Msa0999320:CDS |
| TTACCCTACTGCCAACTTTG+AGG | 0.451034 | 6_4:-81256016 | None:intergenic |
| TACCAAGTAGGCGCATGGTG+TGG | 0.455447 | 6_4:-81255961 | None:intergenic |
| TATATGAGTGTGGCATCATA+TGG | 0.458709 | 6_4:+81255925 | Msa0999320:CDS |
| AAGGACCCCAATGTATATCT+TGG | 0.459225 | 6_4:+81256060 | Msa0999320:CDS |
| ATGTTACTCTGGTCACGAAT+GGG | 0.468552 | 6_4:+81255892 | Msa0999320:CDS |
| TAGATGCCAAGATATACATT+GGG | 0.479422 | 6_4:-81256066 | None:intergenic |
| TGTCCAATTGCACAGCAAAA+GGG | 0.481741 | 6_4:-81256197 | None:intergenic |
| AGGATGATCCTACACGTTGT+TGG | 0.493940 | 6_4:+81255662 | Msa0999320:CDS |
| CCAAGTAGGCGCATGGTGTG+GGG | 0.502089 | 6_4:-81255959 | None:intergenic |
| GCGCCTACTTGGTACGAGAA+TGG | 0.506896 | 6_4:+81255970 | Msa0999320:CDS |
| CGTGACCAGAGTAACATCCT+TGG | 0.510970 | 6_4:-81255886 | None:intergenic |
| GTGACCAGAGTAACATCCTT+GGG | 0.521862 | 6_4:-81255885 | None:intergenic |
| AGGGCAAATGGCCTCAAAGT+TGG | 0.529213 | 6_4:+81256005 | Msa0999320:CDS |
| CCTGCTGCACAAACTTTGCT+GGG | 0.532977 | 6_4:-81255845 | None:intergenic |
| ATAGATGCCAAGATATACAT+TGG | 0.536503 | 6_4:-81256067 | None:intergenic |
| GATGTTACTCTGGTCACGAA+TGG | 0.538013 | 6_4:+81255891 | Msa0999320:CDS |
| TGGCCTCAAAGTTGGCAGTA+GGG | 0.544894 | 6_4:+81256013 | Msa0999320:CDS |
| ATGGCCTCAAAGTTGGCAGT+AGG | 0.546647 | 6_4:+81256012 | Msa0999320:CDS |
| ACAGCAAAAGGGGCATAAAA+CGG | 0.550062 | 6_4:-81256186 | None:intergenic |
| GTCCAATTGCACAGCAAAAG+GGG | 0.566761 | 6_4:-81256196 | None:intergenic |
| TAAGCCCAAGGATGTTACTC+TGG | 0.573695 | 6_4:+81255881 | Msa0999320:CDS |
| TGTTACTCTGGTCACGAATG+GGG | 0.578985 | 6_4:+81255893 | Msa0999320:CDS |
| CCTCCATTCTCGTACCAAGT+AGG | 0.585927 | 6_4:-81255973 | None:intergenic |
| CAAGTAGGCGCATGGTGTGG+GGG | 0.590660 | 6_4:-81255958 | None:intergenic |
| TGGCAGTAGGGTAAAGCTGA+CGG | 0.614821 | 6_4:+81256025 | Msa0999320:CDS |
| GCAAGCGACAGAGAATAAGA+TGG | 0.627502 | 6_4:+81255721 | Msa0999320:CDS |
| TCTCGTACCAAGTAGGCGCA+TGG | 0.632784 | 6_4:-81255966 | None:intergenic |
| GTTACTCTGGTCACGAATGG+GGG | 0.633780 | 6_4:+81255894 | Msa0999320:CDS |
| CTGAATCTCCAACAACGTGT+AGG | 0.646377 | 6_4:-81255670 | None:intergenic |
| CTGACGGCATATCTTGCGAA+AGG | 0.650466 | 6_4:+81256041 | Msa0999320:CDS |
| CCTACTTGGTACGAGAATGG+AGG | 0.658469 | 6_4:+81255973 | Msa0999320:CDS |
| TCTGCTAGAGAATAAGCCCA+AGG | 0.674413 | 6_4:+81255869 | Msa0999320:CDS |
| AGATGCCAAGATATACATTG+GGG | 0.686624 | 6_4:-81256065 | None:intergenic |
| ATTCTCTGTCGCTTGCAATG+CGG | 0.689130 | 6_4:-81255714 | None:intergenic |
| GGAATGCATATATATGAGTG+TGG | 0.712984 | 6_4:+81255915 | Msa0999320:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTTAAATTATCTTCATTT+TGG | + | chr6_4:81256118-81256137 | Msa0999320:exon | 15.0% |
| !!! | AGTTAAATTATCTTCATTTT+GGG | + | chr6_4:81256119-81256138 | Msa0999320:three_prime_UTR | 15.0% |
| !! | AAATGTCGTTGTAATAAATA+AGG | - | chr6_4:81255786-81255805 | None:intergenic | 20.0% |
| TAGATGCCAAGATATACATT+GGG | - | chr6_4:81256069-81256088 | None:intergenic | 30.0% | |
| ATAGATGCCAAGATATACAT+TGG | - | chr6_4:81256070-81256089 | None:intergenic | 30.0% | |
| !!! | CTTCATTTTGGGTTAATATC+TGG | + | chr6_4:81256130-81256149 | Msa0999320:three_prime_UTR | 30.0% |
| GGAATGCATATATATGAGTG+TGG | + | chr6_4:81255915-81255934 | Msa0999320:CDS | 35.0% | |
| TATATGAGTGTGGCATCATA+TGG | + | chr6_4:81255925-81255944 | Msa0999320:CDS | 35.0% | |
| AGATGCCAAGATATACATTG+GGG | - | chr6_4:81256068-81256087 | None:intergenic | 35.0% | |
| ATGTTACTCTGGTCACGAAT+GGG | + | chr6_4:81255892-81255911 | Msa0999320:CDS | 40.0% | |
| ! | AGAATGGAGGCAATTTTGCA+GGG | + | chr6_4:81255986-81256005 | Msa0999320:CDS | 40.0% |
| AAGGACCCCAATGTATATCT+TGG | + | chr6_4:81256060-81256079 | Msa0999320:CDS | 40.0% | |
| ! | ACAGCAAAAGGGGCATAAAA+CGG | - | chr6_4:81256189-81256208 | None:intergenic | 40.0% |
| AGGATGATCCTACACGTTGT+TGG | + | chr6_4:81255662-81255681 | Msa0999320:CDS | 45.0% | |
| CTGAATCTCCAACAACGTGT+AGG | - | chr6_4:81255673-81255692 | None:intergenic | 45.0% | |
| ! | ATTCTCTGTCGCTTGCAATG+CGG | - | chr6_4:81255717-81255736 | None:intergenic | 45.0% |
| GCAAGCGACAGAGAATAAGA+TGG | + | chr6_4:81255721-81255740 | Msa0999320:CDS | 45.0% | |
| TCTGCTAGAGAATAAGCCCA+AGG | + | chr6_4:81255869-81255888 | Msa0999320:CDS | 45.0% | |
| TAAGCCCAAGGATGTTACTC+TGG | + | chr6_4:81255881-81255900 | Msa0999320:CDS | 45.0% | |
| GTGACCAGAGTAACATCCTT+GGG | - | chr6_4:81255888-81255907 | None:intergenic | 45.0% | |
| GATGTTACTCTGGTCACGAA+TGG | + | chr6_4:81255891-81255910 | Msa0999320:CDS | 45.0% | |
| TGTTACTCTGGTCACGAATG+GGG | + | chr6_4:81255893-81255912 | Msa0999320:CDS | 45.0% | |
| ! | GAGAATGGAGGCAATTTTGC+AGG | + | chr6_4:81255985-81256004 | Msa0999320:CDS | 45.0% |
| ! | AGGCAATTTTGCAGGGCAAA+TGG | + | chr6_4:81255993-81256012 | Msa0999320:CDS | 45.0% |
| TTACCCTACTGCCAACTTTG+AGG | - | chr6_4:81256019-81256038 | None:intergenic | 45.0% | |
| GTCCAATTGCACAGCAAAAG+GGG | - | chr6_4:81256199-81256218 | None:intergenic | 45.0% | |
| CCTGCTGCACAAACTTTGCT+GGG | - | chr6_4:81255848-81255867 | None:intergenic | 50.0% | |
| CGTGACCAGAGTAACATCCT+TGG | - | chr6_4:81255889-81255908 | None:intergenic | 50.0% | |
| GTTACTCTGGTCACGAATGG+GGG | + | chr6_4:81255894-81255913 | Msa0999320:CDS | 50.0% | |
| CCTCCATTCTCGTACCAAGT+AGG | - | chr6_4:81255976-81255995 | None:intergenic | 50.0% | |
| CCTACTTGGTACGAGAATGG+AGG | + | chr6_4:81255973-81255992 | Msa0999320:CDS | 50.0% | |
| AGGGCAAATGGCCTCAAAGT+TGG | + | chr6_4:81256005-81256024 | Msa0999320:CDS | 50.0% | |
| ATGGCCTCAAAGTTGGCAGT+AGG | + | chr6_4:81256012-81256031 | Msa0999320:CDS | 50.0% | |
| TGGCCTCAAAGTTGGCAGTA+GGG | + | chr6_4:81256013-81256032 | Msa0999320:CDS | 50.0% | |
| TGGCAGTAGGGTAAAGCTGA+CGG | + | chr6_4:81256025-81256044 | Msa0999320:CDS | 50.0% | |
| CTGACGGCATATCTTGCGAA+AGG | + | chr6_4:81256041-81256060 | Msa0999320:CDS | 50.0% | |
| !! | TGCCCCTTTTGCTGTGCAAT+TGG | + | chr6_4:81256194-81256213 | Msa0999320:three_prime_UTR | 50.0% |
| CCCAGCAAAGTTTGTGCAGC+AGG | + | chr6_4:81255845-81255864 | Msa0999320:CDS | 55.0% | |
| GCCTGCTGCACAAACTTTGC+TGG | - | chr6_4:81255849-81255868 | None:intergenic | 55.0% | |
| ACCAAGTAGGCGCATGGTGT+GGG | - | chr6_4:81255963-81255982 | None:intergenic | 55.0% | |
| TACCAAGTAGGCGCATGGTG+TGG | - | chr6_4:81255964-81255983 | None:intergenic | 55.0% | |
| TCTCGTACCAAGTAGGCGCA+TGG | - | chr6_4:81255969-81255988 | None:intergenic | 55.0% | |
| GCGCCTACTTGGTACGAGAA+TGG | + | chr6_4:81255970-81255989 | Msa0999320:CDS | 55.0% | |
| CAAGTAGGCGCATGGTGTGG+GGG | - | chr6_4:81255961-81255980 | None:intergenic | 60.0% | |
| CCAAGTAGGCGCATGGTGTG+GGG | - | chr6_4:81255962-81255981 | None:intergenic | 60.0% | |
| GCGCATGGTGTGGGGGTTTG+AGG | - | chr6_4:81255954-81255973 | None:intergenic | 65.0% | |
| CCCCACACCATGCGCCTACT+TGG | + | chr6_4:81255959-81255978 | Msa0999320:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 81255662 | 81256218 | 81255662 | ID=Msa0999320;Name=Msa0999320 |
| chr6_4 | mRNA | 81255662 | 81256218 | 81255662 | ID=Msa0999320-mRNA-1;Parent=Msa0999320;Name=Msa0999320-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|1|0.5|1|1|0.5|2|100|119 |
| chr6_4 | exon | 81255662 | 81255742 | 81255662 | ID=Msa0999320-mRNA-1:exon:15519;Parent=Msa0999320-mRNA-1 |
| chr6_4 | exon | 81255840 | 81256218 | 81255840 | ID=Msa0999320-mRNA-1:exon:15520;Parent=Msa0999320-mRNA-1 |
| chr6_4 | CDS | 81255662 | 81255742 | 81255662 | ID=Msa0999320-mRNA-1:cds;Parent=Msa0999320-mRNA-1 |
| chr6_4 | CDS | 81255840 | 81256118 | 81255840 | ID=Msa0999320-mRNA-1:cds;Parent=Msa0999320-mRNA-1 |
| chr6_4 | three_prime_UTR | 81256119 | 81256218 | 81256119 | ID=Msa0999320-mRNA-1:three_prime_utr;Parent=Msa0999320-mRNA-1 |
| Gene Sequence |
| Protein sequence |
