Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa1001870 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006980 | Msa1001870 | 0.809508 | 1.828180e-50 | -8.615850e-47 |
| Msa0055450 | Msa1001870 | 0.857083 | 2.077395e-62 | -8.615850e-47 |
| Msa0669840 | Msa1001870 | 0.814845 | 1.251686e-51 | -8.615850e-47 |
| Msa0725860 | Msa1001870 | 0.807360 | 5.250273e-50 | -8.615850e-47 |
| Msa0739580 | Msa1001870 | 0.842946 | 1.895765e-58 | -8.615850e-47 |
| Msa0782360 | Msa1001870 | 0.817001 | 4.134894e-52 | -8.615850e-47 |
| Msa0819890 | Msa1001870 | 0.807840 | 4.152893e-50 | -8.615850e-47 |
| Msa0823400 | Msa1001870 | 0.802584 | 5.235068e-49 | -8.615850e-47 |
| Msa0124130 | Msa1001870 | 0.861720 | 8.411569e-64 | -8.615850e-47 |
| Msa0141120 | Msa1001870 | 0.828013 | 1.137994e-54 | -8.615850e-47 |
| Msa0149140 | Msa1001870 | 0.824521 | 7.717722e-54 | -8.615850e-47 |
| Msa0212150 | Msa1001870 | 0.806574 | 7.700381e-50 | -8.615850e-47 |
| Msa0406000 | Msa1001870 | 0.841733 | 3.973037e-58 | -8.615850e-47 |
| Msa0424340 | Msa1001870 | 0.828533 | 8.529334e-55 | -8.615850e-47 |
| Msa0424350 | Msa1001870 | 0.831835 | 1.332866e-55 | -8.615850e-47 |
| Msa0451400 | Msa1001870 | 0.842709 | 2.191769e-58 | -8.615850e-47 |
| Msa0999300 | Msa1001870 | 0.820202 | 7.768971e-53 | -8.615850e-47 |
| Msa0999320 | Msa1001870 | 0.999616 | 0.000000e+00 | -8.615850e-47 |
| Msa0999330 | Msa1001870 | 0.803011 | 4.273649e-49 | -8.615850e-47 |
| Msa0999350 | Msa1001870 | 0.819215 | 1.305455e-52 | -8.615850e-47 |
| Msa0999380 | Msa1001870 | 0.817538 | 3.131052e-52 | -8.615850e-47 |
| Msa0999400 | Msa1001870 | 0.810543 | 1.093766e-50 | -8.615850e-47 |
| Msa1001870 | Msa1201160 | 0.818524 | 1.874567e-52 | -8.615850e-47 |
| Msa1001870 | Msa1228190 | 0.805005 | 1.644805e-49 | -8.615850e-47 |
| Msa1001870 | Msa1271260 | 0.805164 | 1.523153e-49 | -8.615850e-47 |
| Msa1001870 | Msa1294290 | 0.821860 | 3.225671e-53 | -8.615850e-47 |
| Msa1001870 | Msa1326630 | 0.817034 | 4.064742e-52 | -8.615850e-47 |
| Msa1001870 | Msa1379970 | 0.807782 | 4.272294e-50 | -8.615850e-47 |
| Msa1001870 | Msa1406780 | 0.837076 | 6.432592e-57 | -8.615850e-47 |
| Msa1001870 | Msa1431850 | 0.846306 | 2.359801e-59 | -8.615850e-47 |
| Msa0252770 | Msa1001870 | 0.813968 | 1.956718e-51 | -8.615850e-47 |
| Msa0306120 | Msa1001870 | 0.817610 | 3.015746e-52 | -8.615850e-47 |
| Msa0329620 | Msa1001870 | 0.813609 | 2.347489e-51 | -8.615850e-47 |
| Msa0343660 | Msa1001870 | 0.811428 | 7.035577e-51 | -8.615850e-47 |
| Msa0359250 | Msa1001870 | 0.829851 | 4.084117e-55 | -8.615850e-47 |
| Msa0492190 | Msa1001870 | 0.827808 | 1.275233e-54 | -8.615850e-47 |
| Msa0515950 | Msa1001870 | 0.817374 | 3.408031e-52 | -8.615850e-47 |
| Msa0591790 | Msa1001870 | 0.812106 | 5.009805e-51 | -8.615850e-47 |
| Msa0591800 | Msa1001870 | 0.810958 | 8.896852e-51 | -8.615850e-47 |
| Msa0860280 | Msa1001870 | 0.829049 | 6.396431e-55 | -8.615850e-47 |
| Msa0898730 | Msa1001870 | 0.810987 | 8.768697e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTTAAATTATCTTCATTT+TGG | 0.128759 | 6_4:-85975907 | Msa1001870:three_prime_UTR |
| GCCTGCTGCACAAACTTTGC+TGG | 0.372514 | 6_4:+85976179 | None:intergenic |
| TTGTCCAATTGCACAGCAAA+AGG | 0.389238 | 6_4:+85975827 | None:intergenic |
| CCCCACACCATGCGCCTACT+TGG | 0.394115 | 6_4:-85976066 | Msa1001870:CDS |
| ACCAAGTAGGCGCATGGTGT+GGG | 0.413182 | 6_4:+85976065 | None:intergenic |
| CCCAGCAAAGTTTGTGCAGC+AGG | 0.418179 | 6_4:-85976180 | Msa1001870:CDS |
| TTACCCTACTGCCAACTTTG+AGG | 0.451034 | 6_4:+85976009 | None:intergenic |
| TACCAAGTAGGCGCATGGTG+TGG | 0.455447 | 6_4:+85976064 | None:intergenic |
| TATATGAGTGTGGCATCATA+TGG | 0.458709 | 6_4:-85976100 | Msa1001870:CDS |
| AAGGACCCCAATGTATATCT+TGG | 0.459225 | 6_4:-85975965 | Msa1001870:CDS |
| ATGTTACTCTGGTCACGAAT+GGG | 0.468552 | 6_4:-85976133 | Msa1001870:CDS |
| TAGATGCCAAGATATACATT+GGG | 0.479422 | 6_4:+85975959 | None:intergenic |
| TGTCCAATTGCACAGCAAAA+GGG | 0.481741 | 6_4:+85975828 | None:intergenic |
| AGGATGATCCTACACGTTGT+TGG | 0.493940 | 6_4:-85976363 | Msa1001870:CDS |
| CCAAGTAGGCGCATGGTGTG+GGG | 0.502089 | 6_4:+85976066 | None:intergenic |
| GCGCCTACTTGGTACGAGAA+TGG | 0.506896 | 6_4:-85976055 | Msa1001870:CDS |
| CGTGACCAGAGTAACATCCT+TGG | 0.510970 | 6_4:+85976139 | None:intergenic |
| GTGACCAGAGTAACATCCTT+GGG | 0.521862 | 6_4:+85976140 | None:intergenic |
| AGGGCAAATGGCCTCAAAGT+TGG | 0.529213 | 6_4:-85976020 | Msa1001870:CDS |
| CCTGCTGCACAAACTTTGCT+GGG | 0.532977 | 6_4:+85976180 | None:intergenic |
| ATAGATGCCAAGATATACAT+TGG | 0.536503 | 6_4:+85975958 | None:intergenic |
| GATGTTACTCTGGTCACGAA+TGG | 0.538013 | 6_4:-85976134 | Msa1001870:CDS |
| TGGCCTCAAAGTTGGCAGTA+GGG | 0.544894 | 6_4:-85976012 | Msa1001870:CDS |
| ATGGCCTCAAAGTTGGCAGT+AGG | 0.546647 | 6_4:-85976013 | Msa1001870:CDS |
| ACAGCAAAAGGGGCATAAAA+CGG | 0.550062 | 6_4:+85975839 | None:intergenic |
| GTCCAATTGCACAGCAAAAG+GGG | 0.566761 | 6_4:+85975829 | None:intergenic |
| TAAGCCCAAGGATGTTACTC+TGG | 0.573695 | 6_4:-85976144 | Msa1001870:CDS |
| TGTTACTCTGGTCACGAATG+GGG | 0.578985 | 6_4:-85976132 | Msa1001870:CDS |
| CCTCCATTCTCGTACCAAGT+AGG | 0.585927 | 6_4:+85976052 | None:intergenic |
| CAAGTAGGCGCATGGTGTGG+GGG | 0.590660 | 6_4:+85976067 | None:intergenic |
| TGGCAGTAGGGTAAAGCTGA+CGG | 0.614821 | 6_4:-85976000 | Msa1001870:CDS |
| GCAAGCGACAGAGAATAAGA+TGG | 0.627502 | 6_4:-85976304 | Msa1001870:intron |
| TCTCGTACCAAGTAGGCGCA+TGG | 0.632784 | 6_4:+85976059 | None:intergenic |
| GTTACTCTGGTCACGAATGG+GGG | 0.633780 | 6_4:-85976131 | Msa1001870:CDS |
| CTGAATCTCCAACAACGTGT+AGG | 0.646377 | 6_4:+85976355 | None:intergenic |
| CTGACGGCATATCTTGCGAA+AGG | 0.650466 | 6_4:-85975984 | Msa1001870:CDS |
| CCTACTTGGTACGAGAATGG+AGG | 0.658469 | 6_4:-85976052 | Msa1001870:CDS |
| TCTGCTAGAGAATAAGCCCA+AGG | 0.674413 | 6_4:-85976156 | Msa1001870:CDS |
| AGATGCCAAGATATACATTG+GGG | 0.686624 | 6_4:+85975960 | None:intergenic |
| ATTCTCTGTCGCTTGCAATG+CGG | 0.689130 | 6_4:+85976311 | None:intergenic |
| GGAATGCATATATATGAGTG+TGG | 0.712984 | 6_4:-85976110 | Msa1001870:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTTAAATTATCTTCATTT+TGG | - | chr6_4:85976282-85976301 | Msa1001870:intron | 15.0% |
| !!! | AGTTAAATTATCTTCATTTT+GGG | - | chr6_4:85976283-85976302 | Msa1001870:intron | 15.0% |
| !! | AAATGTCGTTGTAATAAATA+AGG | + | chr6_4:85975950-85975969 | None:intergenic | 20.0% |
| TAGATGCCAAGATATACATT+GGG | + | chr6_4:85976233-85976252 | None:intergenic | 30.0% | |
| ATAGATGCCAAGATATACAT+TGG | + | chr6_4:85976234-85976253 | None:intergenic | 30.0% | |
| !!! | CTTCATTTTGGGTTAATATC+TGG | - | chr6_4:85976294-85976313 | Msa1001870:intron | 30.0% |
| GGAATGCATATATATGAGTG+TGG | - | chr6_4:85976079-85976098 | Msa1001870:CDS | 35.0% | |
| TATATGAGTGTGGCATCATA+TGG | - | chr6_4:85976089-85976108 | Msa1001870:CDS | 35.0% | |
| AGATGCCAAGATATACATTG+GGG | + | chr6_4:85976232-85976251 | None:intergenic | 35.0% | |
| ATGTTACTCTGGTCACGAAT+GGG | - | chr6_4:85976056-85976075 | Msa1001870:CDS | 40.0% | |
| ! | AGAATGGAGGCAATTTTGCA+GGG | - | chr6_4:85976150-85976169 | Msa1001870:CDS | 40.0% |
| AAGGACCCCAATGTATATCT+TGG | - | chr6_4:85976224-85976243 | Msa1001870:intron | 40.0% | |
| ! | ACAGCAAAAGGGGCATAAAA+CGG | + | chr6_4:85976353-85976372 | None:intergenic | 40.0% |
| CTGAATCTCCAACAACGTGT+AGG | + | chr6_4:85975837-85975856 | None:intergenic | 45.0% | |
| ! | ATTCTCTGTCGCTTGCAATG+CGG | + | chr6_4:85975881-85975900 | None:intergenic | 45.0% |
| GCAAGCGACAGAGAATAAGA+TGG | - | chr6_4:85975885-85975904 | Msa1001870:three_prime_UTR | 45.0% | |
| TCTGCTAGAGAATAAGCCCA+AGG | - | chr6_4:85976033-85976052 | Msa1001870:CDS | 45.0% | |
| TAAGCCCAAGGATGTTACTC+TGG | - | chr6_4:85976045-85976064 | Msa1001870:CDS | 45.0% | |
| GTGACCAGAGTAACATCCTT+GGG | + | chr6_4:85976052-85976071 | None:intergenic | 45.0% | |
| GATGTTACTCTGGTCACGAA+TGG | - | chr6_4:85976055-85976074 | Msa1001870:CDS | 45.0% | |
| TGTTACTCTGGTCACGAATG+GGG | - | chr6_4:85976057-85976076 | Msa1001870:CDS | 45.0% | |
| ! | GAGAATGGAGGCAATTTTGC+AGG | - | chr6_4:85976149-85976168 | Msa1001870:CDS | 45.0% |
| ! | AGGCAATTTTGCAGGGCAAA+TGG | - | chr6_4:85976157-85976176 | Msa1001870:CDS | 45.0% |
| TTACCCTACTGCCAACTTTG+AGG | + | chr6_4:85976183-85976202 | None:intergenic | 45.0% | |
| GTCCAATTGCACAGCAAAAG+GGG | + | chr6_4:85976363-85976382 | None:intergenic | 45.0% | |
| CCTGCTGCACAAACTTTGCT+GGG | + | chr6_4:85976012-85976031 | None:intergenic | 50.0% | |
| CGTGACCAGAGTAACATCCT+TGG | + | chr6_4:85976053-85976072 | None:intergenic | 50.0% | |
| GTTACTCTGGTCACGAATGG+GGG | - | chr6_4:85976058-85976077 | Msa1001870:CDS | 50.0% | |
| CCTCCATTCTCGTACCAAGT+AGG | + | chr6_4:85976140-85976159 | None:intergenic | 50.0% | |
| CCTACTTGGTACGAGAATGG+AGG | - | chr6_4:85976137-85976156 | Msa1001870:CDS | 50.0% | |
| AGGGCAAATGGCCTCAAAGT+TGG | - | chr6_4:85976169-85976188 | Msa1001870:CDS | 50.0% | |
| ATGGCCTCAAAGTTGGCAGT+AGG | - | chr6_4:85976176-85976195 | Msa1001870:CDS | 50.0% | |
| TGGCCTCAAAGTTGGCAGTA+GGG | - | chr6_4:85976177-85976196 | Msa1001870:CDS | 50.0% | |
| TGGCAGTAGGGTAAAGCTGA+CGG | - | chr6_4:85976189-85976208 | Msa1001870:intron | 50.0% | |
| CTGACGGCATATCTTGCGAA+AGG | - | chr6_4:85976205-85976224 | Msa1001870:intron | 50.0% | |
| !! | TGCCCCTTTTGCTGTGCAAT+TGG | - | chr6_4:85976358-85976377 | Msa1001870:CDS | 50.0% |
| CCCAGCAAAGTTTGTGCAGC+AGG | - | chr6_4:85976009-85976028 | Msa1001870:CDS | 55.0% | |
| GCCTGCTGCACAAACTTTGC+TGG | + | chr6_4:85976013-85976032 | None:intergenic | 55.0% | |
| ACCAAGTAGGCGCATGGTGT+GGG | + | chr6_4:85976127-85976146 | None:intergenic | 55.0% | |
| TACCAAGTAGGCGCATGGTG+TGG | + | chr6_4:85976128-85976147 | None:intergenic | 55.0% | |
| TCTCGTACCAAGTAGGCGCA+TGG | + | chr6_4:85976133-85976152 | None:intergenic | 55.0% | |
| GCGCCTACTTGGTACGAGAA+TGG | - | chr6_4:85976134-85976153 | Msa1001870:CDS | 55.0% | |
| CAAGTAGGCGCATGGTGTGG+GGG | + | chr6_4:85976125-85976144 | None:intergenic | 60.0% | |
| CCAAGTAGGCGCATGGTGTG+GGG | + | chr6_4:85976126-85976145 | None:intergenic | 60.0% | |
| GCGCATGGTGTGGGGGTTTG+AGG | + | chr6_4:85976118-85976137 | None:intergenic | 65.0% | |
| CCCCACACCATGCGCCTACT+TGG | - | chr6_4:85976123-85976142 | Msa1001870:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 85975829 | 85976382 | 85975829 | ID=Msa1001870;Name=Msa1001870 |
| chr6_4 | mRNA | 85975829 | 85976382 | 85975829 | ID=Msa1001870-mRNA-1;Parent=Msa1001870;Name=Msa1001870-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|1|0.5|1|1|0.5|2|100|118 |
| chr6_4 | exon | 85975829 | 85976207 | 85975829 | ID=Msa1001870-mRNA-1:exon:16947;Parent=Msa1001870-mRNA-1 |
| chr6_4 | exon | 85976305 | 85976382 | 85976305 | ID=Msa1001870-mRNA-1:exon:16946;Parent=Msa1001870-mRNA-1 |
| chr6_4 | CDS | 85976305 | 85976382 | 85976305 | ID=Msa1001870-mRNA-1:cds;Parent=Msa1001870-mRNA-1 |
| chr6_4 | CDS | 85975929 | 85976207 | 85975929 | ID=Msa1001870-mRNA-1:cds;Parent=Msa1001870-mRNA-1 |
| chr6_4 | three_prime_UTR | 85975829 | 85975928 | 85975829 | ID=Msa1001870-mRNA-1:three_prime_utr;Parent=Msa1001870-mRNA-1 |
| Gene Sequence |
| Protein sequence |
