AlfalfaGEDB | Msa0424350

M. sativa cultivar zhongmu-4 / Msa0424350

KEGG: K03921
GO: GO:0005575 GO:0016020
PFAM: PF03405.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424350	XP_003602146.1	94.262	366	21	0	1	366	1	366	0.0	729

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424350	sp\|Q84VY3\|STAD6_ARATH	68.644	354	106	2	18	366	38	391	0.0	517

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424350	G7J653	94.262	366	21	0	1	366	1	366	0.0	729

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006980	Msa0424350	0.849190	3.792018e-60	-8.615850e-47
Msa0019600	Msa0424350	0.893179	7.993924e-75	-8.615850e-47
Msa0050040	Msa0424350	0.804847	1.774985e-49	-8.615850e-47
Msa0066320	Msa0424350	0.853040	3.107643e-61	-8.615850e-47
Msa0102990	Msa0424350	0.804047	2.605961e-49	-8.615850e-47
Msa0109880	Msa0424350	0.812948	3.278549e-51	-8.615850e-47
Msa0111750	Msa0424350	0.875817	2.284991e-68	-8.615850e-47
Msa0124130	Msa0424350	0.862150	6.214145e-64	-8.615850e-47
Msa0141120	Msa0424350	0.937683	2.326249e-98	-8.615850e-47
Msa0149140	Msa0424350	0.877400	6.476436e-69	-8.615850e-47
Msa0152480	Msa0424350	0.811024	8.608623e-51	-8.615850e-47
Msa0152500	Msa0424350	0.824677	7.092081e-54	-8.615850e-47
Msa0156250	Msa0424350	0.842444	2.576941e-58	-8.615850e-47
Msa0185490	Msa0424350	0.846805	1.724439e-59	-8.615850e-47
Msa0197100	Msa0424350	0.823186	1.586059e-53	-8.615850e-47
Msa0212080	Msa0424350	0.830954	2.195749e-55	-8.615850e-47
Msa0212150	Msa0424350	0.834201	3.437564e-56	-8.615850e-47
Msa0215640	Msa0424350	0.810784	9.705579e-51	-8.615850e-47
Msa0217230	Msa0424350	0.814319	1.637128e-51	-8.615850e-47
Msa0375680	Msa0424350	0.820445	6.832580e-53	-8.615850e-47
Msa0375700	Msa0424350	0.824489	7.853164e-54	-8.615850e-47
Msa0384910	Msa0424350	0.815594	8.533773e-52	-8.615850e-47
Msa0392420	Msa0424350	0.847959	8.311017e-60	-8.615850e-47
Msa0392460	Msa0424350	0.847508	1.106280e-59	-8.615850e-47
Msa0392490	Msa0424350	0.849433	3.243016e-60	-8.615850e-47
Msa0406000	Msa0424350	0.908594	1.441645e-81	-8.615850e-47
Msa0415320	Msa0424350	0.804952	1.687268e-49	-8.615850e-47
Msa0424330	Msa0424350	0.878950	1.854814e-69	-8.615850e-47
Msa0424340	Msa0424350	0.898660	4.268440e-77	-8.615850e-47
Msa0424350	Msa0441590	0.811603	6.445090e-51	-8.615850e-47
Msa0424350	Msa0451400	0.907238	6.290427e-81	-8.615850e-47
Msa0424350	Msa0487570	0.851458	8.760386e-61	-8.615850e-47
Msa0424350	Msa0487630	0.844023	9.769303e-59	-8.615850e-47
Msa0424350	Msa0492190	0.890630	8.277690e-74	-8.615850e-47
Msa0424350	Msa0515950	0.870018	2.002437e-66	-8.615850e-47
Msa0424350	Msa0534930	0.862575	4.598317e-64	-8.615850e-47
Msa0424350	Msa0535040	0.841488	4.611015e-58	-8.615850e-47
Msa0424350	Msa0538220	0.800425	1.449791e-48	-8.615850e-47
Msa0424350	Msa0591790	0.865445	5.875830e-65	-8.615850e-47
Msa0424350	Msa0594090	0.857159	1.974077e-62	-8.615850e-47
Msa0424350	Msa0641250	0.821450	4.010866e-53	-8.615850e-47
Msa0424350	Msa0669840	0.885575	7.224151e-72	-8.615850e-47
Msa0424350	Msa0707220	0.803510	3.367977e-49	-8.615850e-47
Msa0424350	Msa0709450	0.823333	1.465298e-53	-8.615850e-47
Msa0424350	Msa0711320	0.838089	3.537061e-57	-8.615850e-47
Msa0424350	Msa0725860	0.811480	6.855365e-51	-8.615850e-47
Msa0424350	Msa0739580	0.883305	5.019164e-71	-8.615850e-47
Msa0424350	Msa0748400	0.841524	4.510917e-58	-8.615850e-47
Msa0424350	Msa0751890	0.835628	1.501843e-56	-8.615850e-47
Msa0424350	Msa0752840	0.835629	1.501600e-56	-8.615850e-47
Msa0424350	Msa0764820	0.821725	3.464910e-53	-8.615850e-47
Msa0424350	Msa0770290	0.817051	4.029333e-52	-8.615850e-47
Msa0424350	Msa0782360	0.854538	1.151778e-61	-8.615850e-47
Msa0424350	Msa0819890	0.819166	1.339694e-52	-8.615850e-47
Msa0424350	Msa0823400	0.804203	2.418022e-49	-8.615850e-47
Msa0424350	Msa0828830	0.816102	6.576051e-52	-8.615850e-47
Msa0424350	Msa0831940	0.845234	4.611296e-59	-8.615850e-47
Msa0424350	Msa0849280	0.801410	9.125573e-49	-8.615850e-47
Msa0424350	Msa0851020	0.807044	6.126854e-50	-8.615850e-47
Msa0424350	Msa0860280	0.859630	3.621734e-63	-8.615850e-47
Msa0424350	Msa0867350	0.861041	1.354881e-63	-8.615850e-47
Msa0424350	Msa0896350	0.807334	5.317024e-50	-8.615850e-47
Msa0424350	Msa0898730	0.877242	7.352077e-69	-8.615850e-47
Msa0424350	Msa0916640	0.842664	2.252224e-58	-8.615850e-47
Msa0424350	Msa0922750	0.825483	4.573159e-54	-8.615850e-47
Msa0424350	Msa0927120	0.818224	2.191976e-52	-8.615850e-47
Msa0424350	Msa0929870	0.849816	2.536958e-60	-8.615850e-47
Msa0424350	Msa0941820	0.802954	4.389732e-49	-8.615850e-47
Msa0424350	Msa0944760	0.844714	6.371025e-59	-8.615850e-47
Msa0424350	Msa0944880	0.820106	8.173216e-53	-8.615850e-47
Msa0424350	Msa0953540	0.866221	3.341287e-65	-8.615850e-47
Msa0424350	Msa0961560	0.833564	4.960720e-56	-8.615850e-47
Msa0424350	Msa0996270	0.832102	1.144760e-55	-8.615850e-47
Msa0424350	Msa0999300	0.860239	2.372923e-63	-8.615850e-47
Msa0424350	Msa0999320	0.829987	3.783736e-55	-8.615850e-47
Msa0424350	Msa1001870	0.831835	1.332866e-55	-8.615850e-47
Msa0424350	Msa1001890	0.828634	8.059487e-55	-8.615850e-47
Msa0424350	Msa1037890	0.858126	1.020020e-62	-8.615850e-47
Msa0424350	Msa1037900	0.837219	5.915157e-57	-8.615850e-47
Msa0424350	Msa1037910	0.823526	1.320942e-53	-8.615850e-47
Msa0424350	Msa1081570	0.808349	3.235865e-50	-8.615850e-47
Msa0424350	Msa1084520	0.867535	1.273726e-65	-8.615850e-47
Msa0424350	Msa1125810	0.833674	4.655909e-56	-8.615850e-47
Msa0424350	Msa1127140	0.809668	1.688717e-50	-8.615850e-47
Msa0424350	Msa1129950	0.839254	1.768811e-57	-8.615850e-47
Msa0424350	Msa1129960	0.826916	2.086197e-54	-8.615850e-47
Msa0424350	Msa1129970	0.822305	2.542752e-53	-8.615850e-47
Msa0424350	Msa1130010	0.849932	2.353165e-60	-8.615850e-47
Msa0424350	Msa1145020	0.824237	9.001409e-54	-8.615850e-47
Msa0424350	Msa1166400	0.807801	4.233024e-50	-8.615850e-47
Msa0424350	Msa1171310	0.847678	9.935363e-60	-8.615850e-47
Msa0424350	Msa1201110	0.804683	1.920754e-49	-8.615850e-47
Msa0424350	Msa1201160	0.850710	1.424072e-60	-8.615850e-47
Msa0424350	Msa1228190	0.838394	2.952682e-57	-8.615850e-47
Msa0424350	Msa1294290	0.831122	1.996572e-55	-8.615850e-47
Msa0424350	Msa1305510	0.860013	2.777261e-63	-8.615850e-47
Msa0424350	Msa1326630	0.842108	3.161717e-58	-8.615850e-47
Msa0424350	Msa1356740	0.801309	9.570314e-49	-8.615850e-47
Msa0424350	Msa1374550	0.811260	7.652313e-51	-8.615850e-47
Msa0424350	Msa1378870	0.833378	5.521062e-56	-8.615850e-47
Msa0424350	Msa1379970	0.818137	2.293080e-52	-8.615850e-47
Msa0424350	Msa1394890	0.849468	3.171332e-60	-8.615850e-47
Msa0424350	Msa1406780	0.874251	7.812439e-68	-8.615850e-47
Msa0424350	Msa1421550	0.857767	1.303969e-62	-8.615850e-47
Msa0424350	Msa1423460	0.849281	3.575181e-60	-8.615850e-47
Msa0424350	Msa1431850	0.877986	4.046167e-69	-8.615850e-47
Msa0424350	Msa1455320	0.834960	2.215290e-56	-8.615850e-47
Msa0424350	Msa1459530	0.830364	3.061951e-55	-8.615850e-47
Msa0424350	Msa1461060	0.849518	3.071050e-60	-8.615850e-47
Msa0239030	Msa0424350	0.834082	3.681545e-56	-8.615850e-47
Msa0252630	Msa0424350	0.837531	4.920500e-57	-8.615850e-47
Msa0252770	Msa0424350	0.838432	2.885813e-57	-8.615850e-47
Msa0265420	Msa0424350	0.801287	9.669255e-49	-8.615850e-47
Msa0276740	Msa0424350	0.822486	2.308729e-53	-8.615850e-47
Msa0289920	Msa0424350	0.818356	2.046207e-52	-8.615850e-47
Msa0291330	Msa0424350	0.815111	1.092886e-51	-8.615850e-47
Msa0306120	Msa0424350	0.846821	1.706968e-59	-8.615850e-47
Msa0316680	Msa0424350	0.829218	5.820764e-55	-8.615850e-47
Msa0329620	Msa0424350	0.839013	2.041746e-57	-8.615850e-47
Msa0333700	Msa0424350	0.813415	2.589228e-51	-8.615850e-47
Msa0334700	Msa0424350	0.809157	2.173361e-50	-8.615850e-47
Msa0343660	Msa0424350	0.834653	2.646074e-56	-8.615850e-47
Msa0344570	Msa0424350	0.837606	4.706805e-57	-8.615850e-47
Msa0351770	Msa0424350	0.839561	1.472231e-57	-8.615850e-47
Msa0359250	Msa0424350	0.901916	1.649869e-78	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424350	MtrunA17_Chr3g0126011	94.262	366	21	0	1	366	1	366	0.0	729
Msa0424350	MtrunA17_Chr3g0125991	90.984	366	33	0	1	366	1	366	0.0	704
Msa0424350	MtrunA17_Chr3g0125781	78.962	366	73	1	5	366	4	369	0.0	607
Msa0424350	MtrunA17_Chr3g0125981	75.204	367	86	1	3	364	2	368	0.0	583
Msa0424350	MtrunA17_Chr1g0159321	74.128	344	86	1	26	366	47	390	0.0	547
Msa0424350	MtrunA17_Chr4g0045661	65.143	350	114	3	23	366	46	393	9.79e-171	481
Msa0424350	MtrunA17_Chr5g0406961	65.143	350	114	3	23	366	97	444	1.80e-170	482

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424350	AT1G43800.1	68.644	354	106	2	18	366	38	391	0.0	517
Msa0424350	AT3G02630.1	64.793	338	115	2	29	364	59	394	2.44e-166	470
Msa0424350	AT5G16240.1	63.037	349	120	3	23	364	46	392	1.16e-165	469
Msa0424350	AT3G02610.2	59.677	372	130	5	14	366	43	413	1.08e-162	462
Msa0424350	AT3G02610.1	59.677	372	130	5	14	366	41	411	1.42e-162	461
Msa0424350	AT2G43710.2	63.422	339	121	2	29	366	65	401	2.25e-162	461
Msa0424350	AT2G43710.1	63.422	339	121	2	29	366	65	401	2.92e-162	460
Msa0424350	AT3G02620.1	59.890	364	134	4	14	366	41	403	4.85e-162	460
Msa0424350	AT3G02620.2	59.890	364	134	4	14	366	44	406	5.74e-162	459
Msa0424350	AT5G16230.1	61.696	342	126	3	29	366	61	401	5.53e-160	454
Msa0424350	AT5G16230.2	61.963	326	119	3	45	366	1	325	8.67e-154	436
Msa0424350	AT3G02620.3	61.890	328	118	3	45	366	1	327	1.24e-152	433

Find 98 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATGATTATCTTGTGGTTT+TGG	0.263671	3_2:-74554074	Msa0424350:CDS
GAGACATTGAGTGTGTCTTT+TGG	0.299881	3_2:+74554275	None:intergenic
AATCTTGTGCCGCATCATTT+TGG	0.331259	3_2:+74552940	None:intergenic
CCAACTTGTTTATCCATGTT+TGG	0.336928	3_2:+74554014	None:intergenic
CGCCAGCTCCAATCAAATAT+TGG	0.345449	3_2:+74553840	None:intergenic
GTCTCACACCATGGGCTATA+TGG	0.350432	3_2:-74553964	Msa0424350:CDS
TTCTCAGGGCCTGCACTTGA+TGG	0.351234	3_2:+74554116	None:intergenic
GTGGACTTGCACCTAGATTA+AGG	0.352719	3_2:-74552724	Msa0424350:CDS
GGGCTATATGGTCACGCTCT+TGG	0.370720	3_2:-74553952	Msa0424350:CDS
TGCACCTAGATTAAGGAAAC+TGG	0.374627	3_2:-74552717	Msa0424350:CDS
TGTTCAAATCACTAGAGAAA+TGG	0.378630	3_2:-74554237	Msa0424350:CDS
CTTGATTAGACGATGGAGAT+TGG	0.392791	3_2:-74552798	Msa0424350:CDS
GCTCAGCCGTTCGATTTCTC+AGG	0.393347	3_2:+74554101	None:intergenic
GTTCAAATCACTAGAGAAAT+GGG	0.399384	3_2:-74554236	Msa0424350:CDS
AAGACTATCCAATATTTGAT+TGG	0.400329	3_2:-74553848	Msa0424350:CDS
TGAAGATCCAAGAGAATTTG+TGG	0.410017	3_2:+74552660	None:intergenic
TCTGCAGTGGCACAGCGTAT+TGG	0.420298	3_2:-74552860	Msa0424350:CDS
GGCTGCCAGCATTCCTCCAC+AGG	0.422913	3_2:+74554184	None:intergenic
GTGTACACAAAACCCATGTA+TGG	0.438769	3_2:+74553154	None:intergenic
GGGCCTTTAGATAACATTGT+AGG	0.442941	3_2:+74554320	None:intergenic
GATGGAGTTGGCGACGAGAC+TGG	0.447459	3_2:-74553992	Msa0424350:CDS
TGGAGATTGGAGAAGATTGA+GGG	0.448075	3_2:-74552785	Msa0424350:CDS
TCTTCCAGTTTCCTTAATCT+AGG	0.451053	3_2:+74552713	None:intergenic
AAACTGGAAGAGCGTCTTGA+TGG	0.452571	3_2:-74552701	Msa0424350:CDS
GGCCTTTAGATAACATTGTA+GGG	0.464982	3_2:+74554321	None:intergenic
TACCGAAAACAACCCATACA+TGG	0.467155	3_2:-74553167	Msa0424350:CDS
AGATCAACCACAAATTCTCT+TGG	0.467703	3_2:-74552667	Msa0424350:CDS
CTCTCACTTGCACGCGTATG+CGG	0.471157	3_2:-74553061	Msa0424350:CDS
TAAGATATCAACGTAATCAT+TGG	0.471259	3_2:+74552826	None:intergenic
ATCCAATATTTGATTGGAGC+TGG	0.472360	3_2:-74553842	Msa0424350:CDS
AGTGGATCCCACCGAGGCTA+TGG	0.482931	3_2:-74552972	Msa0424350:CDS
ATGGAGATTGGAGAAGATTG+AGG	0.484391	3_2:-74552786	Msa0424350:CDS
GGCTATGGCCACCATAGCCT+CGG	0.489653	3_2:+74552961	None:intergenic
TCTCTTTAGCCAACCTACCT+AGG	0.494048	3_2:+74553092	None:intergenic
TTCACAAAATGTGTTGCCAT+TGG	0.497194	3_2:-74554212	Msa0424350:CDS
AAAACCACAAGATAATCATC+TGG	0.498146	3_2:+74554076	None:intergenic
AACTGGAAGAGCGTCTTGAT+GGG	0.500840	3_2:-74552700	Msa0424350:CDS
TTTATCCATGTTTGGTACGT+AGG	0.501588	3_2:+74554022	None:intergenic
CCTGCTCACTTGATGCATGA+TGG	0.509740	3_2:-74552911	Msa0424350:CDS
GTACGTAGGCAATGCTTCCT+CGG	0.510676	3_2:+74554036	None:intergenic
TCGGAAGGTAGTGATGAGTC+TGG	0.523237	3_2:+74554148	None:intergenic
ATCATATTACAACAAAGATC+AGG	0.525928	3_2:+74552475	None:intergenic
TCCCCTACAATGTTATCTAA+AGG	0.527355	3_2:-74554323	Msa0424350:CDS
GCTTCCAGATGATTATCTTG+TGG	0.527931	3_2:-74554080	Msa0424350:CDS
CCATCATGCATCAAGTGAGC+AGG	0.528607	3_2:+74552911	None:intergenic
TCTCACTTGCACGCGTATGC+GGG	0.529845	3_2:-74553060	Msa0424350:CDS
CCAAACATGGATAAACAAGT+TGG	0.530351	3_2:-74554014	Msa0424350:CDS
AAAACTTATCTATATCTCTC+CGG	0.537755	3_2:-74553896	Msa0424350:CDS
CATTGCCTACGTACCAAACA+TGG	0.538689	3_2:-74554027	Msa0424350:CDS
AAATGTGGCTCGCTCTTGAA+AGG	0.540932	3_2:+74553129	None:intergenic
TTAAACCTGTGGAGGAATGC+TGG	0.548548	3_2:-74554189	Msa0424350:CDS
CTCAGCCGTTCGATTTCTCA+GGG	0.548721	3_2:+74554102	None:intergenic
ATTGTAGGGGAGTCCACGCA+TGG	0.558263	3_2:+74554335	None:intergenic
ATAAACAAGTTGGATGGAGT+TGG	0.559959	3_2:-74554004	Msa0424350:CDS
CGCATACGCGTGCAAGTGAG+AGG	0.560592	3_2:+74553062	None:intergenic
GGATCCCACCGAGGCTATGG+TGG	0.562827	3_2:-74552969	Msa0424350:CDS
TGTGGAAAAGCTTCTAGAAG+TGG	0.566245	3_2:-74552990	Msa0424350:CDS
TGAGTTCATCCATCAAGTGC+AGG	0.567672	3_2:-74554125	Msa0424350:CDS
TCCTCCACAGGTTTAACCAA+TGG	0.568150	3_2:+74554196	None:intergenic
TATGGCCACCATAGCCTCGG+TGG	0.570103	3_2:+74552964	None:intergenic
ACATGGATAAACAAGTTGGA+TGG	0.572710	3_2:-74554010	Msa0424350:CDS
ATTGAGGGTTTGACAAGCGA+AGG	0.578019	3_2:-74552770	Msa0424350:CDS
TTGTTCATGGTGTAGGATGT+GGG	0.582554	3_2:-74553190	Msa0424350:intron
TTTATACAAAGACATAGTGT+AGG	0.582555	3_2:-74552568	Msa0424350:three_prime_UTR
ATATTTGATTGGAGCTGGCG+TGG	0.585908	3_2:-74553837	Msa0424350:intron
CATGCACATATCAACTCGAC+CGG	0.587049	3_2:+74553877	None:intergenic
CAACATTATGCACCCATGCG+TGG	0.592760	3_2:-74554348	Msa0424350:CDS
TGGTTCAAGTCTCACACCAT+GGG	0.600203	3_2:-74553972	Msa0424350:CDS
TAGAGTTCTTGATTAGACGA+TGG	0.605110	3_2:-74552805	Msa0424350:CDS
TTTGTGTCACACGGAAACCT+AGG	0.610294	3_2:-74553109	Msa0424350:CDS
ACACGGAAACCTAGGTAGGT+TGG	0.610946	3_2:-74553101	Msa0424350:CDS
CTGGTTCAAGTCTCACACCA+TGG	0.612232	3_2:-74553973	Msa0424350:CDS
CGAGCCACATTTGTGTCACA+CGG	0.618843	3_2:-74553118	Msa0424350:CDS
ATGGCCACCATAGCCTCGGT+GGG	0.619166	3_2:+74552965	None:intergenic
GCCTTTAGATAACATTGTAG+GGG	0.623075	3_2:+74554322	None:intergenic
GAGCGTGACCATATAGCCCA+TGG	0.625365	3_2:+74553956	None:intergenic
ACCGAAAACAACCCATACAT+GGG	0.628237	3_2:-74553166	Msa0424350:CDS
TATCAAATTAACAATGACGA+TGG	0.630822	3_2:-74554371	Msa0424350:exon
GCCATTGGTTAAACCTGTGG+AGG	0.632502	3_2:-74554197	Msa0424350:CDS
GGTAGGTTGGCTAAAGAGAG+AGG	0.635441	3_2:-74553088	Msa0424350:CDS
TGTACACAAAACCCATGTAT+GGG	0.636404	3_2:+74553155	None:intergenic
GAATGCATATGTGAGGATTG+TGG	0.642224	3_2:-74553008	Msa0424350:CDS
TGTCACACGGAAACCTAGGT+AGG	0.642596	3_2:-74553105	Msa0424350:CDS
TTGTAGGGGAGTCCACGCAT+GGG	0.642906	3_2:+74554336	None:intergenic
GTTGCCATTGGTTAAACCTG+TGG	0.644205	3_2:-74554200	Msa0424350:CDS
TGCAACTATAAAACTTCACA+AGG	0.648856	3_2:+74552625	None:intergenic
CATGCACAAGAGTTCGTGTG+TGG	0.649922	3_2:-74552743	Msa0424350:CDS
GAGTCTGGCAACAAATTATG+TGG	0.650290	3_2:+74554163	None:intergenic
GCAGGCCCTGAGAAATCGAA+CGG	0.653102	3_2:-74554107	Msa0424350:CDS
CACTTGATGGATGAACTCAT+CGG	0.663477	3_2:+74554129	None:intergenic
GTCACGAGAATGCATATGTG+AGG	0.664895	3_2:-74553015	Msa0424350:CDS
ACCGCTGAAGAAAATAGACA+TGG	0.664992	3_2:-74553929	Msa0424350:CDS
CTCGTGACGCTTCTCATCAG+CGG	0.665409	3_2:+74553030	None:intergenic
TGATGGATGAACTCATCGGA+AGG	0.673548	3_2:+74554133	None:intergenic
GTTTCCGTGTGACACAAATG+TGG	0.677033	3_2:+74553114	None:intergenic
TGGCCATAGCCAAAATGATG+CGG	0.688297	3_2:-74552949	Msa0424350:CDS
TCTAGAAGTGGATCCCACCG+AGG	0.721401	3_2:-74552978	Msa0424350:CDS
GGTCGGTGGCTTGATCACCG+AGG	0.746193	3_2:-74554053	Msa0424350:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTATGAAAAATGTTATT+TGG	+	chr3_2:74553628-74553647	None:intergenic	10.0%
!!	AATTTATGAAAAATGTTATT+TGG	+	chr3_2:74553628-74553647	None:intergenic	10.0%
!!	TAATAAATCATATTTGTGTA+TGG	+	chr3_2:74553319-74553338	None:intergenic	15.0%
!!	ATAAATTAATTGATTGTTCA+TGG	-	chr3_2:74553641-74553660	Msa0424350:intron	15.0%
!!	TAATAAATCATATTTGTGTA+TGG	+	chr3_2:74553319-74553338	None:intergenic	15.0%
!!	ATAAATTAATTGATTGTTCA+TGG	-	chr3_2:74553641-74553660	Msa0424350:intron	15.0%
!!	TAATGACAATGCATAATATA+TGG	-	chr3_2:74553436-74553455	Msa0424350:intron	20.0%
!!	AATGACAATGCATAATATAT+GGG	-	chr3_2:74553437-74553456	Msa0424350:intron	20.0%
!!	AACGACTATTAAAAAAATAG+TGG	+	chr3_2:74553499-74553518	None:intergenic	20.0%
!!	TAATGACAATGCATAATATA+TGG	-	chr3_2:74553436-74553455	Msa0424350:intron	20.0%
!!	AATGACAATGCATAATATAT+GGG	-	chr3_2:74553437-74553456	Msa0424350:intron	20.0%
!!	AACGACTATTAAAAAAATAG+TGG	+	chr3_2:74553499-74553518	None:intergenic	20.0%
!	TATCAAATTAACAATGACGA+TGG	-	chr3_2:74552473-74552492	Msa0424350:three_prime_UTR	25.0%
!	AAAACTTATCTATATCTCTC+CGG	-	chr3_2:74552948-74552967	Msa0424350:CDS	25.0%
!	AAGACTATCCAATATTTGAT+TGG	-	chr3_2:74552996-74553015	Msa0424350:CDS	25.0%
!!!	ATTTGTTATTCTCGTTAGTT+TGG	+	chr3_2:74553221-74553240	None:intergenic	25.0%
!	ATGACAATGCATAATATATG+GGG	-	chr3_2:74553438-74553457	Msa0424350:intron	25.0%
!	TAAGATGTGATAGATAGAAT+AGG	+	chr3_2:74553568-74553587	None:intergenic	25.0%
!!	TATTTCTATTTCTTTCTCCT+TGG	-	chr3_2:74553586-74553605	Msa0424350:intron	25.0%
!	TAAGATATCAACGTAATCAT+TGG	+	chr3_2:74554021-74554040	None:intergenic	25.0%
!	TTTATACAAAGACATAGTGT+AGG	-	chr3_2:74554276-74554295	Msa0424350:CDS	25.0%
!	ATCATATTACAACAAAGATC+AGG	+	chr3_2:74554372-74554391	None:intergenic	25.0%
!	TATCAAATTAACAATGACGA+TGG	-	chr3_2:74552473-74552492	Msa0424350:three_prime_UTR	25.0%
!	AAAACTTATCTATATCTCTC+CGG	-	chr3_2:74552948-74552967	Msa0424350:CDS	25.0%
!	AAGACTATCCAATATTTGAT+TGG	-	chr3_2:74552996-74553015	Msa0424350:CDS	25.0%
!!!	ATTTGTTATTCTCGTTAGTT+TGG	+	chr3_2:74553221-74553240	None:intergenic	25.0%
!	ATGACAATGCATAATATATG+GGG	-	chr3_2:74553438-74553457	Msa0424350:intron	25.0%
!	TAAGATGTGATAGATAGAAT+AGG	+	chr3_2:74553568-74553587	None:intergenic	25.0%
!!	TATTTCTATTTCTTTCTCCT+TGG	-	chr3_2:74553586-74553605	Msa0424350:intron	25.0%
!	TAAGATATCAACGTAATCAT+TGG	+	chr3_2:74554021-74554040	None:intergenic	25.0%
!	TTTATACAAAGACATAGTGT+AGG	-	chr3_2:74554276-74554295	Msa0424350:CDS	25.0%
!	ATCATATTACAACAAAGATC+AGG	+	chr3_2:74554372-74554391	None:intergenic	25.0%
!!	TTTTAGGAATGTTTTGGATG+GGG	+	chr3_2:74552547-74552566	None:intergenic	30.0%
!!!	GTTTTAGGAATGTTTTGGAT+GGG	+	chr3_2:74552548-74552567	None:intergenic	30.0%
!	TTGAACACTTCTTTCTTTTC+AGG	+	chr3_2:74552594-74552613	None:intergenic	30.0%
	TGTTCAAATCACTAGAGAAA+TGG	-	chr3_2:74552607-74552626	Msa0424350:three_prime_UTR	30.0%
	GTTCAAATCACTAGAGAAAT+GGG	-	chr3_2:74552608-74552627	Msa0424350:three_prime_UTR	30.0%
	AAAACCACAAGATAATCATC+TGG	+	chr3_2:74552771-74552790	None:intergenic	30.0%
!	AGATGATTATCTTGTGGTTT+TGG	-	chr3_2:74552770-74552789	Msa0424350:CDS	30.0%
	ACATATACATGCACACAATA+TGG	-	chr3_2:74553068-74553087	Msa0424350:CDS	30.0%
	TGACAATGCATAATATATGG+GGG	-	chr3_2:74553439-74553458	Msa0424350:intron	30.0%
	ATGTGATAGATAGAATAGGT+TGG	+	chr3_2:74553564-74553583	None:intergenic	30.0%
	TAATTGATTGTTCATGGTGT+AGG	-	chr3_2:74553647-74553666	Msa0424350:intron	30.0%
	TGCAACTATAAAACTTCACA+AGG	+	chr3_2:74554222-74554241	None:intergenic	30.0%
!!	TTTTAGGAATGTTTTGGATG+GGG	+	chr3_2:74552547-74552566	None:intergenic	30.0%
!!!	GTTTTAGGAATGTTTTGGAT+GGG	+	chr3_2:74552548-74552567	None:intergenic	30.0%
!	TTGAACACTTCTTTCTTTTC+AGG	+	chr3_2:74552594-74552613	None:intergenic	30.0%
	TGTTCAAATCACTAGAGAAA+TGG	-	chr3_2:74552607-74552626	Msa0424350:three_prime_UTR	30.0%
	GTTCAAATCACTAGAGAAAT+GGG	-	chr3_2:74552608-74552627	Msa0424350:three_prime_UTR	30.0%
	AAAACCACAAGATAATCATC+TGG	+	chr3_2:74552771-74552790	None:intergenic	30.0%
!	AGATGATTATCTTGTGGTTT+TGG	-	chr3_2:74552770-74552789	Msa0424350:CDS	30.0%
	ACATATACATGCACACAATA+TGG	-	chr3_2:74553068-74553087	Msa0424350:CDS	30.0%
	TGACAATGCATAATATATGG+GGG	-	chr3_2:74553439-74553458	Msa0424350:intron	30.0%
	ATGTGATAGATAGAATAGGT+TGG	+	chr3_2:74553564-74553583	None:intergenic	30.0%
	TAATTGATTGTTCATGGTGT+AGG	-	chr3_2:74553647-74553666	Msa0424350:intron	30.0%
	TGCAACTATAAAACTTCACA+AGG	+	chr3_2:74554222-74554241	None:intergenic	30.0%
	TCCCCTACAATGTTATCTAA+AGG	-	chr3_2:74552521-74552540	Msa0424350:three_prime_UTR	35.0%
	GCCTTTAGATAACATTGTAG+GGG	+	chr3_2:74552525-74552544	None:intergenic	35.0%
	GGCCTTTAGATAACATTGTA+GGG	+	chr3_2:74552526-74552545	None:intergenic	35.0%
!!!	GGTTTTAGGAATGTTTTGGA+TGG	+	chr3_2:74552549-74552568	None:intergenic	35.0%
!!!	TTGGGGTTTTAGGAATGTTT+TGG	+	chr3_2:74552553-74552572	None:intergenic	35.0%
!	AGACATTGAGTGTGTCTTTT+GGG	+	chr3_2:74552571-74552590	None:intergenic	35.0%
	TTCACAAAATGTGTTGCCAT+TGG	-	chr3_2:74552632-74552651	Msa0424350:exon	35.0%
!!!	GATTATCTTGTGGTTTTGGT+CGG	-	chr3_2:74552774-74552793	Msa0424350:CDS	35.0%
	TTTATCCATGTTTGGTACGT+AGG	+	chr3_2:74552825-74552844	None:intergenic	35.0%
!	CCAACTTGTTTATCCATGTT+TGG	+	chr3_2:74552833-74552852	None:intergenic	35.0%
	CCAAACATGGATAAACAAGT+TGG	-	chr3_2:74552830-74552849	Msa0424350:CDS	35.0%
!	ACATGGATAAACAAGTTGGA+TGG	-	chr3_2:74552834-74552853	Msa0424350:CDS	35.0%
!	ATAAACAAGTTGGATGGAGT+TGG	-	chr3_2:74552840-74552859	Msa0424350:CDS	35.0%
!	TCCATGTCTATTTTCTTCAG+CGG	+	chr3_2:74552919-74552938	None:intergenic	35.0%
	ATCCAATATTTGATTGGAGC+TGG	-	chr3_2:74553002-74553021	Msa0424350:CDS	35.0%
!	CGTTAGTTTGGCAAATGTAT+TGG	+	chr3_2:74553209-74553228	None:intergenic	35.0%
	GACAATGCATAATATATGGG+GGG	-	chr3_2:74553440-74553459	Msa0424350:intron	35.0%
!	AGTGGTGTCTTTTCTTTTTG+CGG	+	chr3_2:74553481-74553500	None:intergenic	35.0%
!	TTCTATTTCTTTCTCCTTGG+AGG	-	chr3_2:74553589-74553608	Msa0424350:intron	35.0%
!	TCTATTTCTTTCTCCTTGGA+GGG	-	chr3_2:74553590-74553609	Msa0424350:intron	35.0%
	TGTACACAAAACCCATGTAT+GGG	+	chr3_2:74553692-74553711	None:intergenic	35.0%
	TAGAGTTCTTGATTAGACGA+TGG	-	chr3_2:74554039-74554058	Msa0424350:CDS	35.0%
	TCTTCCAGTTTCCTTAATCT+AGG	+	chr3_2:74554134-74554153	None:intergenic	35.0%
	AGATCAACCACAAATTCTCT+TGG	-	chr3_2:74554177-74554196	Msa0424350:CDS	35.0%
	TGAAGATCCAAGAGAATTTG+TGG	+	chr3_2:74554187-74554206	None:intergenic	35.0%
	TCCCCTACAATGTTATCTAA+AGG	-	chr3_2:74552521-74552540	Msa0424350:three_prime_UTR	35.0%
	GCCTTTAGATAACATTGTAG+GGG	+	chr3_2:74552525-74552544	None:intergenic	35.0%
	GGCCTTTAGATAACATTGTA+GGG	+	chr3_2:74552526-74552545	None:intergenic	35.0%
!!!	GGTTTTAGGAATGTTTTGGA+TGG	+	chr3_2:74552549-74552568	None:intergenic	35.0%
!!!	TTGGGGTTTTAGGAATGTTT+TGG	+	chr3_2:74552553-74552572	None:intergenic	35.0%
!	AGACATTGAGTGTGTCTTTT+GGG	+	chr3_2:74552571-74552590	None:intergenic	35.0%
	TTCACAAAATGTGTTGCCAT+TGG	-	chr3_2:74552632-74552651	Msa0424350:exon	35.0%
!!!	GATTATCTTGTGGTTTTGGT+CGG	-	chr3_2:74552774-74552793	Msa0424350:CDS	35.0%
	TTTATCCATGTTTGGTACGT+AGG	+	chr3_2:74552825-74552844	None:intergenic	35.0%
!	CCAACTTGTTTATCCATGTT+TGG	+	chr3_2:74552833-74552852	None:intergenic	35.0%
	CCAAACATGGATAAACAAGT+TGG	-	chr3_2:74552830-74552849	Msa0424350:CDS	35.0%
!	ACATGGATAAACAAGTTGGA+TGG	-	chr3_2:74552834-74552853	Msa0424350:CDS	35.0%
!	ATAAACAAGTTGGATGGAGT+TGG	-	chr3_2:74552840-74552859	Msa0424350:CDS	35.0%
!	TCCATGTCTATTTTCTTCAG+CGG	+	chr3_2:74552919-74552938	None:intergenic	35.0%
	ATCCAATATTTGATTGGAGC+TGG	-	chr3_2:74553002-74553021	Msa0424350:CDS	35.0%
!	CGTTAGTTTGGCAAATGTAT+TGG	+	chr3_2:74553209-74553228	None:intergenic	35.0%
	GACAATGCATAATATATGGG+GGG	-	chr3_2:74553440-74553459	Msa0424350:intron	35.0%
!	AGTGGTGTCTTTTCTTTTTG+CGG	+	chr3_2:74553481-74553500	None:intergenic	35.0%
!	TTCTATTTCTTTCTCCTTGG+AGG	-	chr3_2:74553589-74553608	Msa0424350:intron	35.0%
!	TCTATTTCTTTCTCCTTGGA+GGG	-	chr3_2:74553590-74553609	Msa0424350:intron	35.0%
	TGTACACAAAACCCATGTAT+GGG	+	chr3_2:74553692-74553711	None:intergenic	35.0%
	TAGAGTTCTTGATTAGACGA+TGG	-	chr3_2:74554039-74554058	Msa0424350:CDS	35.0%
	TCTTCCAGTTTCCTTAATCT+AGG	+	chr3_2:74554134-74554153	None:intergenic	35.0%
	AGATCAACCACAAATTCTCT+TGG	-	chr3_2:74554177-74554196	Msa0424350:CDS	35.0%
	TGAAGATCCAAGAGAATTTG+TGG	+	chr3_2:74554187-74554206	None:intergenic	35.0%
	GGGCCTTTAGATAACATTGT+AGG	+	chr3_2:74552527-74552546	None:intergenic	40.0%
!!!	AGTGTGTCTTTTGGGGTTTT+AGG	+	chr3_2:74552563-74552582	None:intergenic	40.0%
!	GACATTGAGTGTGTCTTTTG+GGG	+	chr3_2:74552570-74552589	None:intergenic	40.0%
!	GAGACATTGAGTGTGTCTTT+TGG	+	chr3_2:74552572-74552591	None:intergenic	40.0%
	GAGTCTGGCAACAAATTATG+TGG	+	chr3_2:74552684-74552703	None:intergenic	40.0%
!	CACTTGATGGATGAACTCAT+CGG	+	chr3_2:74552718-74552737	None:intergenic	40.0%
	GCTTCCAGATGATTATCTTG+TGG	-	chr3_2:74552764-74552783	Msa0424350:CDS	40.0%
	ACCGCTGAAGAAAATAGACA+TGG	-	chr3_2:74552915-74552934	Msa0424350:CDS	40.0%
	AGCTCACGAGGACAAAATAT+AGG	+	chr3_2:74553404-74553423	None:intergenic	40.0%
!	CTATTTCTTTCTCCTTGGAG+GGG	-	chr3_2:74553591-74553610	Msa0424350:intron	40.0%
	ATTGTTCATGGTGTAGGATG+TGG	-	chr3_2:74553653-74553672	Msa0424350:intron	40.0%
	TTGTTCATGGTGTAGGATGT+GGG	-	chr3_2:74553654-74553673	Msa0424350:intron	40.0%
	TACCGAAAACAACCCATACA+TGG	-	chr3_2:74553677-74553696	Msa0424350:intron	40.0%
	ACCGAAAACAACCCATACAT+GGG	-	chr3_2:74553678-74553697	Msa0424350:intron	40.0%
!!	ACCCATGTATGGGTTGTTTT+CGG	+	chr3_2:74553682-74553701	None:intergenic	40.0%
	GTGTACACAAAACCCATGTA+TGG	+	chr3_2:74553693-74553712	None:intergenic	40.0%
	GAATGCATATGTGAGGATTG+TGG	-	chr3_2:74553836-74553855	Msa0424350:intron	40.0%
!	TGTGGAAAAGCTTCTAGAAG+TGG	-	chr3_2:74553854-74553873	Msa0424350:CDS	40.0%
!	AATCTTGTGCCGCATCATTT+TGG	+	chr3_2:74553907-74553926	None:intergenic	40.0%
!	AAAAATGATCGAAGAGGCGT+GGG	+	chr3_2:74553965-74553984	None:intergenic	40.0%
	CTGCAGAAAAATGATCGAAG+AGG	+	chr3_2:74553971-74553990	None:intergenic	40.0%
!!	CTTCGATCATTTTTCTGCAG+TGG	-	chr3_2:74553971-74553990	Msa0424350:CDS	40.0%
	CTTGATTAGACGATGGAGAT+TGG	-	chr3_2:74554046-74554065	Msa0424350:CDS	40.0%
	ATGGAGATTGGAGAAGATTG+AGG	-	chr3_2:74554058-74554077	Msa0424350:CDS	40.0%
	TGGAGATTGGAGAAGATTGA+GGG	-	chr3_2:74554059-74554078	Msa0424350:CDS	40.0%
	TGCACCTAGATTAAGGAAAC+TGG	-	chr3_2:74554127-74554146	Msa0424350:CDS	40.0%
	GGGCCTTTAGATAACATTGT+AGG	+	chr3_2:74552527-74552546	None:intergenic	40.0%
!!!	AGTGTGTCTTTTGGGGTTTT+AGG	+	chr3_2:74552563-74552582	None:intergenic	40.0%
!	GACATTGAGTGTGTCTTTTG+GGG	+	chr3_2:74552570-74552589	None:intergenic	40.0%
!	GAGACATTGAGTGTGTCTTT+TGG	+	chr3_2:74552572-74552591	None:intergenic	40.0%
	GAGTCTGGCAACAAATTATG+TGG	+	chr3_2:74552684-74552703	None:intergenic	40.0%
!	CACTTGATGGATGAACTCAT+CGG	+	chr3_2:74552718-74552737	None:intergenic	40.0%
	GCTTCCAGATGATTATCTTG+TGG	-	chr3_2:74552764-74552783	Msa0424350:CDS	40.0%
	ACCGCTGAAGAAAATAGACA+TGG	-	chr3_2:74552915-74552934	Msa0424350:CDS	40.0%
	AGCTCACGAGGACAAAATAT+AGG	+	chr3_2:74553404-74553423	None:intergenic	40.0%
!	CTATTTCTTTCTCCTTGGAG+GGG	-	chr3_2:74553591-74553610	Msa0424350:intron	40.0%
	ATTGTTCATGGTGTAGGATG+TGG	-	chr3_2:74553653-74553672	Msa0424350:intron	40.0%
	TTGTTCATGGTGTAGGATGT+GGG	-	chr3_2:74553654-74553673	Msa0424350:intron	40.0%
	TACCGAAAACAACCCATACA+TGG	-	chr3_2:74553677-74553696	Msa0424350:intron	40.0%
	ACCGAAAACAACCCATACAT+GGG	-	chr3_2:74553678-74553697	Msa0424350:intron	40.0%
!!	ACCCATGTATGGGTTGTTTT+CGG	+	chr3_2:74553682-74553701	None:intergenic	40.0%
	GTGTACACAAAACCCATGTA+TGG	+	chr3_2:74553693-74553712	None:intergenic	40.0%
	GAATGCATATGTGAGGATTG+TGG	-	chr3_2:74553836-74553855	Msa0424350:intron	40.0%
!	TGTGGAAAAGCTTCTAGAAG+TGG	-	chr3_2:74553854-74553873	Msa0424350:CDS	40.0%
!	AATCTTGTGCCGCATCATTT+TGG	+	chr3_2:74553907-74553926	None:intergenic	40.0%
!	AAAAATGATCGAAGAGGCGT+GGG	+	chr3_2:74553965-74553984	None:intergenic	40.0%
	CTGCAGAAAAATGATCGAAG+AGG	+	chr3_2:74553971-74553990	None:intergenic	40.0%
!!	CTTCGATCATTTTTCTGCAG+TGG	-	chr3_2:74553971-74553990	Msa0424350:CDS	40.0%
	CTTGATTAGACGATGGAGAT+TGG	-	chr3_2:74554046-74554065	Msa0424350:CDS	40.0%
	ATGGAGATTGGAGAAGATTG+AGG	-	chr3_2:74554058-74554077	Msa0424350:CDS	40.0%
	TGGAGATTGGAGAAGATTGA+GGG	-	chr3_2:74554059-74554078	Msa0424350:CDS	40.0%
	TGCACCTAGATTAAGGAAAC+TGG	-	chr3_2:74554127-74554146	Msa0424350:CDS	40.0%
!	GTTGCCATTGGTTAAACCTG+TGG	-	chr3_2:74552644-74552663	Msa0424350:CDS	45.0%
	TCCTCCACAGGTTTAACCAA+TGG	+	chr3_2:74552651-74552670	None:intergenic	45.0%
	TTAAACCTGTGGAGGAATGC+TGG	-	chr3_2:74552655-74552674	Msa0424350:CDS	45.0%
!	TGATGGATGAACTCATCGGA+AGG	+	chr3_2:74552714-74552733	None:intergenic	45.0%
	TGAGTTCATCCATCAAGTGC+AGG	-	chr3_2:74552719-74552738	Msa0424350:CDS	45.0%
!!!	TATCTTGTGGTTTTGGTCGG+TGG	-	chr3_2:74552777-74552796	Msa0424350:CDS	45.0%
	CATTGCCTACGTACCAAACA+TGG	-	chr3_2:74552817-74552836	Msa0424350:CDS	45.0%
	TGGTTCAAGTCTCACACCAT+GGG	-	chr3_2:74552872-74552891	Msa0424350:CDS	45.0%
	CATGCACATATCAACTCGAC+CGG	+	chr3_2:74552970-74552989	None:intergenic	45.0%
	CGCCAGCTCCAATCAAATAT+TGG	+	chr3_2:74553007-74553026	None:intergenic	45.0%
	ATATTTGATTGGAGCTGGCG+TGG	-	chr3_2:74553007-74553026	Msa0424350:CDS	45.0%
	TGCACTCGTAACACGTATGT+GGG	+	chr3_2:74553287-74553306	None:intergenic	45.0%
	TATATGGGGGGTTCAAATCC+CGG	-	chr3_2:74553452-74553471	Msa0424350:intron	45.0%
!	TCTTTTCTTTTTGCGGTGGC+CGG	+	chr3_2:74553474-74553493	None:intergenic	45.0%
!	GGTGTCTTTTCTTTTTGCGG+TGG	+	chr3_2:74553478-74553497	None:intergenic	45.0%
	AAATGTGGCTCGCTCTTGAA+AGG	+	chr3_2:74553718-74553737	None:intergenic	45.0%
	GTTTCCGTGTGACACAAATG+TGG	+	chr3_2:74553733-74553752	None:intergenic	45.0%
	TTTGTGTCACACGGAAACCT+AGG	-	chr3_2:74553735-74553754	Msa0424350:intron	45.0%
	TCTCTTTAGCCAACCTACCT+AGG	+	chr3_2:74553755-74553774	None:intergenic	45.0%
	GTCACGAGAATGCATATGTG+AGG	-	chr3_2:74553829-74553848	Msa0424350:intron	45.0%
	TGGCCATAGCCAAAATGATG+CGG	-	chr3_2:74553895-74553914	Msa0424350:CDS	45.0%
	GAAAAATGATCGAAGAGGCG+TGG	+	chr3_2:74553966-74553985	None:intergenic	45.0%
	ATTGAGGGTTTGACAAGCGA+AGG	-	chr3_2:74554074-74554093	Msa0424350:CDS	45.0%
	GTGGACTTGCACCTAGATTA+AGG	-	chr3_2:74554120-74554139	Msa0424350:CDS	45.0%
!!	AAACTGGAAGAGCGTCTTGA+TGG	-	chr3_2:74554143-74554162	Msa0424350:CDS	45.0%
!!	AACTGGAAGAGCGTCTTGAT+GGG	-	chr3_2:74554144-74554163	Msa0424350:CDS	45.0%
!	GTTGCCATTGGTTAAACCTG+TGG	-	chr3_2:74552644-74552663	Msa0424350:CDS	45.0%
	TCCTCCACAGGTTTAACCAA+TGG	+	chr3_2:74552651-74552670	None:intergenic	45.0%
	TTAAACCTGTGGAGGAATGC+TGG	-	chr3_2:74552655-74552674	Msa0424350:CDS	45.0%
!	TGATGGATGAACTCATCGGA+AGG	+	chr3_2:74552714-74552733	None:intergenic	45.0%
	TGAGTTCATCCATCAAGTGC+AGG	-	chr3_2:74552719-74552738	Msa0424350:CDS	45.0%
!!!	TATCTTGTGGTTTTGGTCGG+TGG	-	chr3_2:74552777-74552796	Msa0424350:CDS	45.0%
	CATTGCCTACGTACCAAACA+TGG	-	chr3_2:74552817-74552836	Msa0424350:CDS	45.0%
	TGGTTCAAGTCTCACACCAT+GGG	-	chr3_2:74552872-74552891	Msa0424350:CDS	45.0%
	CATGCACATATCAACTCGAC+CGG	+	chr3_2:74552970-74552989	None:intergenic	45.0%
	CGCCAGCTCCAATCAAATAT+TGG	+	chr3_2:74553007-74553026	None:intergenic	45.0%
	ATATTTGATTGGAGCTGGCG+TGG	-	chr3_2:74553007-74553026	Msa0424350:CDS	45.0%
	TGCACTCGTAACACGTATGT+GGG	+	chr3_2:74553287-74553306	None:intergenic	45.0%
	TATATGGGGGGTTCAAATCC+CGG	-	chr3_2:74553452-74553471	Msa0424350:intron	45.0%
!	TCTTTTCTTTTTGCGGTGGC+CGG	+	chr3_2:74553474-74553493	None:intergenic	45.0%
!	GGTGTCTTTTCTTTTTGCGG+TGG	+	chr3_2:74553478-74553497	None:intergenic	45.0%
	AAATGTGGCTCGCTCTTGAA+AGG	+	chr3_2:74553718-74553737	None:intergenic	45.0%
	GTTTCCGTGTGACACAAATG+TGG	+	chr3_2:74553733-74553752	None:intergenic	45.0%
	TTTGTGTCACACGGAAACCT+AGG	-	chr3_2:74553735-74553754	Msa0424350:intron	45.0%
	TCTCTTTAGCCAACCTACCT+AGG	+	chr3_2:74553755-74553774	None:intergenic	45.0%
	GTCACGAGAATGCATATGTG+AGG	-	chr3_2:74553829-74553848	Msa0424350:intron	45.0%
	TGGCCATAGCCAAAATGATG+CGG	-	chr3_2:74553895-74553914	Msa0424350:CDS	45.0%
	GAAAAATGATCGAAGAGGCG+TGG	+	chr3_2:74553966-74553985	None:intergenic	45.0%
	ATTGAGGGTTTGACAAGCGA+AGG	-	chr3_2:74554074-74554093	Msa0424350:CDS	45.0%
	GTGGACTTGCACCTAGATTA+AGG	-	chr3_2:74554120-74554139	Msa0424350:CDS	45.0%
!!	AAACTGGAAGAGCGTCTTGA+TGG	-	chr3_2:74554143-74554162	Msa0424350:CDS	45.0%
!!	AACTGGAAGAGCGTCTTGAT+GGG	-	chr3_2:74554144-74554163	Msa0424350:CDS	45.0%
	CAACATTATGCACCCATGCG+TGG	-	chr3_2:74552496-74552515	Msa0424350:three_prime_UTR	50.0%
!	GCCATTGGTTAAACCTGTGG+AGG	-	chr3_2:74552647-74552666	Msa0424350:CDS	50.0%
	TCGGAAGGTAGTGATGAGTC+TGG	+	chr3_2:74552699-74552718	None:intergenic	50.0%
	CTCAGCCGTTCGATTTCTCA+GGG	+	chr3_2:74552745-74552764	None:intergenic	50.0%
	GTACGTAGGCAATGCTTCCT+CGG	+	chr3_2:74552811-74552830	None:intergenic	50.0%
	CTGGTTCAAGTCTCACACCA+TGG	-	chr3_2:74552871-74552890	Msa0424350:CDS	50.0%
!	GTCTCACACCATGGGCTATA+TGG	-	chr3_2:74552880-74552899	Msa0424350:CDS	50.0%
	CTGCACTCGTAACACGTATG+TGG	+	chr3_2:74553288-74553307	None:intergenic	50.0%
	CACATACGTGTTACGAGTGC+AGG	-	chr3_2:74553286-74553305	Msa0424350:intron	50.0%
	CCAACTGAGTTAAGCTCACG+AGG	+	chr3_2:74553416-74553435	None:intergenic	50.0%
	CCTCGTGAGCTTAACTCAGT+TGG	-	chr3_2:74553413-74553432	Msa0424350:intron	50.0%
!	CTTTTCTTTTTGCGGTGGCC+GGG	+	chr3_2:74553473-74553492	None:intergenic	50.0%
	GACATATGCATACCCCTCCA+AGG	+	chr3_2:74553606-74553625	None:intergenic	50.0%
	CGAGCCACATTTGTGTCACA+CGG	-	chr3_2:74553726-74553745	Msa0424350:intron	50.0%
	TGTCACACGGAAACCTAGGT+AGG	-	chr3_2:74553739-74553758	Msa0424350:intron	50.0%
	ACACGGAAACCTAGGTAGGT+TGG	-	chr3_2:74553743-74553762	Msa0424350:intron	50.0%
!	GGTAGGTTGGCTAAAGAGAG+AGG	-	chr3_2:74553756-74553775	Msa0424350:intron	50.0%
!!!	GTGCCGCATCATTTTGGCTA+TGG	+	chr3_2:74553901-74553920	None:intergenic	50.0%
	CCATCATGCATCAAGTGAGC+AGG	+	chr3_2:74553936-74553955	None:intergenic	50.0%
	CCTGCTCACTTGATGCATGA+TGG	-	chr3_2:74553933-74553952	Msa0424350:CDS	50.0%
	CATGCACAAGAGTTCGTGTG+TGG	-	chr3_2:74554101-74554120	Msa0424350:CDS	50.0%
	CAACATTATGCACCCATGCG+TGG	-	chr3_2:74552496-74552515	Msa0424350:three_prime_UTR	50.0%
!	GCCATTGGTTAAACCTGTGG+AGG	-	chr3_2:74552647-74552666	Msa0424350:CDS	50.0%
	TCGGAAGGTAGTGATGAGTC+TGG	+	chr3_2:74552699-74552718	None:intergenic	50.0%
	CTCAGCCGTTCGATTTCTCA+GGG	+	chr3_2:74552745-74552764	None:intergenic	50.0%
	GTACGTAGGCAATGCTTCCT+CGG	+	chr3_2:74552811-74552830	None:intergenic	50.0%
	CTGGTTCAAGTCTCACACCA+TGG	-	chr3_2:74552871-74552890	Msa0424350:CDS	50.0%
!	GTCTCACACCATGGGCTATA+TGG	-	chr3_2:74552880-74552899	Msa0424350:CDS	50.0%
	CTGCACTCGTAACACGTATG+TGG	+	chr3_2:74553288-74553307	None:intergenic	50.0%
	CACATACGTGTTACGAGTGC+AGG	-	chr3_2:74553286-74553305	Msa0424350:intron	50.0%
	CCAACTGAGTTAAGCTCACG+AGG	+	chr3_2:74553416-74553435	None:intergenic	50.0%
	CCTCGTGAGCTTAACTCAGT+TGG	-	chr3_2:74553413-74553432	Msa0424350:intron	50.0%
!	CTTTTCTTTTTGCGGTGGCC+GGG	+	chr3_2:74553473-74553492	None:intergenic	50.0%
	GACATATGCATACCCCTCCA+AGG	+	chr3_2:74553606-74553625	None:intergenic	50.0%
	CGAGCCACATTTGTGTCACA+CGG	-	chr3_2:74553726-74553745	Msa0424350:intron	50.0%
	TGTCACACGGAAACCTAGGT+AGG	-	chr3_2:74553739-74553758	Msa0424350:intron	50.0%
	ACACGGAAACCTAGGTAGGT+TGG	-	chr3_2:74553743-74553762	Msa0424350:intron	50.0%
!	GGTAGGTTGGCTAAAGAGAG+AGG	-	chr3_2:74553756-74553775	Msa0424350:intron	50.0%
!!!	GTGCCGCATCATTTTGGCTA+TGG	+	chr3_2:74553901-74553920	None:intergenic	50.0%
	CCATCATGCATCAAGTGAGC+AGG	+	chr3_2:74553936-74553955	None:intergenic	50.0%
	CCTGCTCACTTGATGCATGA+TGG	-	chr3_2:74553933-74553952	Msa0424350:CDS	50.0%
	CATGCACAAGAGTTCGTGTG+TGG	-	chr3_2:74554101-74554120	Msa0424350:CDS	50.0%
	TTGTAGGGGAGTCCACGCAT+GGG	+	chr3_2:74552511-74552530	None:intergenic	55.0%
	ATTGTAGGGGAGTCCACGCA+TGG	+	chr3_2:74552512-74552531	None:intergenic	55.0%
	TTCTCAGGGCCTGCACTTGA+TGG	+	chr3_2:74552731-74552750	None:intergenic	55.0%
	GCAGGCCCTGAGAAATCGAA+CGG	-	chr3_2:74552737-74552756	Msa0424350:CDS	55.0%
	GCTCAGCCGTTCGATTTCTC+AGG	+	chr3_2:74552746-74552765	None:intergenic	55.0%
	GAGCGTGACCATATAGCCCA+TGG	+	chr3_2:74552891-74552910	None:intergenic	55.0%
	GGGCTATATGGTCACGCTCT+TGG	-	chr3_2:74552892-74552911	Msa0424350:CDS	55.0%
	CTCTCACTTGCACGCGTATG+CGG	-	chr3_2:74553783-74553802	Msa0424350:intron	55.0%
	TCTCACTTGCACGCGTATGC+GGG	-	chr3_2:74553784-74553803	Msa0424350:intron	55.0%
	CTCGTGACGCTTCTCATCAG+CGG	+	chr3_2:74553817-74553836	None:intergenic	55.0%
	TCTAGAAGTGGATCCCACCG+AGG	-	chr3_2:74553866-74553885	Msa0424350:CDS	55.0%
	TCTGCAGTGGCACAGCGTAT+TGG	-	chr3_2:74553984-74554003	Msa0424350:CDS	55.0%
	TTGTAGGGGAGTCCACGCAT+GGG	+	chr3_2:74552511-74552530	None:intergenic	55.0%
	ATTGTAGGGGAGTCCACGCA+TGG	+	chr3_2:74552512-74552531	None:intergenic	55.0%
	TTCTCAGGGCCTGCACTTGA+TGG	+	chr3_2:74552731-74552750	None:intergenic	55.0%
	GCAGGCCCTGAGAAATCGAA+CGG	-	chr3_2:74552737-74552756	Msa0424350:CDS	55.0%
	GCTCAGCCGTTCGATTTCTC+AGG	+	chr3_2:74552746-74552765	None:intergenic	55.0%
	GAGCGTGACCATATAGCCCA+TGG	+	chr3_2:74552891-74552910	None:intergenic	55.0%
	GGGCTATATGGTCACGCTCT+TGG	-	chr3_2:74552892-74552911	Msa0424350:CDS	55.0%
	CTCTCACTTGCACGCGTATG+CGG	-	chr3_2:74553783-74553802	Msa0424350:intron	55.0%
	TCTCACTTGCACGCGTATGC+GGG	-	chr3_2:74553784-74553803	Msa0424350:intron	55.0%
	CTCGTGACGCTTCTCATCAG+CGG	+	chr3_2:74553817-74553836	None:intergenic	55.0%
	TCTAGAAGTGGATCCCACCG+AGG	-	chr3_2:74553866-74553885	Msa0424350:CDS	55.0%
	TCTGCAGTGGCACAGCGTAT+TGG	-	chr3_2:74553984-74554003	Msa0424350:CDS	55.0%
	GATGGAGTTGGCGACGAGAC+TGG	-	chr3_2:74552852-74552871	Msa0424350:CDS	60.0%
	CGCATACGCGTGCAAGTGAG+AGG	+	chr3_2:74553785-74553804	None:intergenic	60.0%
	AGTGGATCCCACCGAGGCTA+TGG	-	chr3_2:74553872-74553891	Msa0424350:CDS	60.0%
	ATGGCCACCATAGCCTCGGT+GGG	+	chr3_2:74553882-74553901	None:intergenic	60.0%
	TATGGCCACCATAGCCTCGG+TGG	+	chr3_2:74553883-74553902	None:intergenic	60.0%
	GGCTATGGCCACCATAGCCT+CGG	+	chr3_2:74553886-74553905	None:intergenic	60.0%
	GATGGAGTTGGCGACGAGAC+TGG	-	chr3_2:74552852-74552871	Msa0424350:CDS	60.0%
	CGCATACGCGTGCAAGTGAG+AGG	+	chr3_2:74553785-74553804	None:intergenic	60.0%
	AGTGGATCCCACCGAGGCTA+TGG	-	chr3_2:74553872-74553891	Msa0424350:CDS	60.0%
	ATGGCCACCATAGCCTCGGT+GGG	+	chr3_2:74553882-74553901	None:intergenic	60.0%
	TATGGCCACCATAGCCTCGG+TGG	+	chr3_2:74553883-74553902	None:intergenic	60.0%
	GGCTATGGCCACCATAGCCT+CGG	+	chr3_2:74553886-74553905	None:intergenic	60.0%
	GGCTGCCAGCATTCCTCCAC+AGG	+	chr3_2:74552663-74552682	None:intergenic	65.0%
	GGTCGGTGGCTTGATCACCG+AGG	-	chr3_2:74552791-74552810	Msa0424350:CDS	65.0%
	GGATCCCACCGAGGCTATGG+TGG	-	chr3_2:74553875-74553894	Msa0424350:CDS	65.0%
	GGCTGCCAGCATTCCTCCAC+AGG	+	chr3_2:74552663-74552682	None:intergenic	65.0%
	GGTCGGTGGCTTGATCACCG+AGG	-	chr3_2:74552791-74552810	Msa0424350:CDS	65.0%
	GGATCCCACCGAGGCTATGG+TGG	-	chr3_2:74553875-74553894	Msa0424350:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	74552468	74554398	74552468	ID=Msa0424350;Name=Msa0424350
chr3_2	mRNA	74552468	74554398	74552468	ID=Msa0424350-mRNA-1;Parent=Msa0424350;Name=Msa0424350-mRNA-1;_AED=0.11;_eAED=0.11;_QI=18\|1\|1\|1\|1\|1\|2\|172\|366
chr3_2	exon	74552468	74553197	74552468	ID=Msa0424350-mRNA-1:exon:18811;Parent=Msa0424350-mRNA-1
chr3_2	exon	74553838	74554398	74553838	ID=Msa0424350-mRNA-1:exon:18810;Parent=Msa0424350-mRNA-1
chr3_2	five_prime_UTR	74554381	74554398	74554381	ID=Msa0424350-mRNA-1:five_prime_utr;Parent=Msa0424350-mRNA-1
chr3_2	CDS	74553838	74554380	74553838	ID=Msa0424350-mRNA-1:cds;Parent=Msa0424350-mRNA-1
chr3_2	CDS	74552640	74553197	74552640	ID=Msa0424350-mRNA-1:cds;Parent=Msa0424350-mRNA-1
chr3_2	three_prime_UTR	74552468	74552639	74552468	ID=Msa0424350-mRNA-1:three_prime_utr;Parent=Msa0424350-mRNA-1

Gene Sequence

>Msa0424350

ATGACGATGGCAACATTATGCACCCATGCGTGGACTCCCCTACAATGTTATCTAAAGGCCCCATCCAAAACATTCCTAAAACCCCAAAAGACACACTCAATGTCTCCTGAAAAGAAAGAAGTGTTCAAATCACTAGAGAAATGGGTTTCACAAAATGTGTTGCCATTGGTTAAACCTGTGGAGGAATGCTGGCAGCCACATAATTTGTTGCCAGACTCATCACTACCTTCCGATGAGTTCATCCATCAAGTGCAGGCCCTGAGAAATCGAACGGCTGAGCTTCCAGATGATTATCTTGTGGTTTTGGTCGGTGGCTTGATCACCGAGGAAGCATTGCCTACGTACCAAACATGGATAAACAAGTTGGATGGAGTTGGCGACGAGACTGGTTCAAGTCTCACACCATGGGCTATATGGTCACGCTCTTGGACCGCTGAAGAAAATAGACATGGAGATTTACTCAAAACTTATCTATATCTCTCCGGTCGAGTTGATATGTGCATGATTGAGAAGACTATCCAATATTTGATTGGAGCTGGCGTGGATGTGGGTACCGAAAACAACCCATACATGGGTTTTGTGTACACATCCTTTCAAGAGCGAGCCACATTTGTGTCACACGGAAACCTAGGTAGGTTGGCTAAAGAGAGAGGTGACCTCTCACTTGCACGCGTATGCGGGACAATTGCCGCTGATGAGAAGCGTCACGAGAATGCATATGTGAGGATTGTGGAAAAGCTTCTAGAAGTGGATCCCACCGAGGCTATGGTGGCCATAGCCAAAATGATGCGGCACAAGATTACAATGCCTGCTCACTTGATGCATGATGGACAAGACCCACGCCTCTTCGATCATTTTTCTGCAGTGGCACAGCGTATTGGCGCGTACACGACCAATGATTACGTTGATATCTTAGAGTTCTTGATTAGACGATGGAGATTGGAGAAGATTGAGGGTTTGACAAGCGAAGGACAACATGCACAAGAGTTCGTGTGTGGACTTGCACCTAGATTAAGGAAACTGGAAGAGCGTCTTGATGGGAGAACAAGCAAGATCAACCACAAATTCTCTTGGATCTTCAATAAAGAGTTGTCCTTGTGA

Protein sequence

>Msa0424350

MTMATLCTHAWTPLQCYLKAPSKTFLKPQKTHSMSPEKKEVFKSLEKWVSQNVLPLVKPVEECWQPHNLLPDSSLPSDEFIHQVQALRNRTAELPDDYLVVLVGGLITEEALPTYQTWINKLDGVGDETGSSLTPWAIWSRSWTAEENRHGDLLKTYLYLSGRVDMCMIEKTIQYLIGAGVDVGTENNPYMGFVYTSFQERATFVSHGNLGRLAKERGDLSLARVCGTIAADEKRHENAYVRIVEKLLEVDPTEAMVAIAKMMRHKITMPAHLMHDGQDPRLFDHFSAVAQRIGAYTTNDYVDILEFLIRRWRLEKIEGLTSEGQHAQEFVCGLAPRLRKLEERLDGRTSKINHKFSWIFNKELSL*