Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045050 | XP_036856100.1 | 93.204 | 103 | 7 | 0 | 1 | 103 | 1 | 103 | 2.56e-48 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045050 | sp|P0CG89|H4_SOYBN | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 4.92e-50 | 156 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045050 | A0A453II90 | 94.175 | 103 | 6 | 0 | 1 | 103 | 42 | 144 | 6.34e-49 | 161 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0020390 | Msa1045050 | 0.820729 | 5.881504e-53 | -8.615850e-47 |
| Msa0118540 | Msa1045050 | 0.821005 | 5.080691e-53 | -8.615850e-47 |
| Msa0615580 | Msa1045050 | 0.800347 | 1.504267e-48 | -8.615850e-47 |
| Msa0669720 | Msa1045050 | 0.814239 | 1.704464e-51 | -8.615850e-47 |
| Msa0672480 | Msa1045050 | 0.808047 | 3.751764e-50 | -8.615850e-47 |
| Msa0748920 | Msa1045050 | 0.805160 | 1.526775e-49 | -8.615850e-47 |
| Msa0800370 | Msa1045050 | 0.816508 | 5.333068e-52 | -8.615850e-47 |
| Msa0816730 | Msa1045050 | 0.804489 | 2.108310e-49 | -8.615850e-47 |
| Msa0826150 | Msa1045050 | 0.800639 | 1.311190e-48 | -8.615850e-47 |
| Msa0833190 | Msa1045050 | -0.809367 | 1.959528e-50 | -8.615850e-47 |
| Msa0183850 | Msa1045050 | 0.812125 | 4.962068e-51 | -8.615850e-47 |
| Msa0189520 | Msa1045050 | 0.800918 | 1.150234e-48 | -8.615850e-47 |
| Msa0202410 | Msa1045050 | 0.801261 | 9.785736e-49 | -8.615850e-47 |
| Msa0207670 | Msa1045050 | 0.804239 | 2.376499e-49 | -8.615850e-47 |
| Msa0209500 | Msa1045050 | 0.811425 | 7.047716e-51 | -8.615850e-47 |
| Msa0233380 | Msa1045050 | 0.809589 | 1.756440e-50 | -8.615850e-47 |
| Msa0365860 | Msa1045050 | 0.826758 | 2.276022e-54 | -8.615850e-47 |
| Msa0385490 | Msa1045050 | 0.807337 | 5.310880e-50 | -8.615850e-47 |
| Msa0436920 | Msa1045050 | -0.821338 | 4.257126e-53 | -8.615850e-47 |
| Msa0463340 | Msa1045050 | 0.819987 | 8.703443e-53 | -8.615850e-47 |
| Msa0467220 | Msa1045050 | 0.800684 | 1.284057e-48 | -8.615850e-47 |
| Msa0473830 | Msa1045050 | -0.802322 | 5.927800e-49 | -8.615850e-47 |
| Msa0994370 | Msa1045050 | 0.813790 | 2.141366e-51 | -8.615850e-47 |
| Msa1034290 | Msa1045050 | 0.800536 | 1.376560e-48 | -8.615850e-47 |
| Msa1045050 | Msa1088290 | 0.804168 | 2.459360e-49 | -8.615850e-47 |
| Msa1045050 | Msa1133230 | 0.814970 | 1.174348e-51 | -8.615850e-47 |
| Msa1045050 | Msa1133250 | 0.818895 | 1.543761e-52 | -8.615850e-47 |
| Msa1045050 | Msa1165780 | 0.813513 | 2.463980e-51 | -8.615850e-47 |
| Msa1045050 | Msa1254730 | 0.806225 | 9.120454e-50 | -8.615850e-47 |
| Msa1045050 | Msa1275110 | 0.802833 | 4.649778e-49 | -8.615850e-47 |
| Msa1045050 | Msa1296830 | 0.807798 | 4.238581e-50 | -8.615850e-47 |
| Msa1045050 | Msa1307600 | 0.800872 | 1.175316e-48 | -8.615850e-47 |
| Msa1045050 | Msa1336560 | 0.815973 | 7.026305e-52 | -8.615850e-47 |
| Msa1045050 | Msa1351540 | 0.844021 | 9.780763e-59 | -8.615850e-47 |
| Msa1045050 | Msa1359270 | 0.808969 | 2.384772e-50 | -8.615850e-47 |
| Msa1045050 | Msa1416370 | 0.830628 | 2.638916e-55 | -8.615850e-47 |
| Msa1045050 | Msa1422810 | 0.817588 | 3.049949e-52 | -8.615850e-47 |
| Msa1045050 | Msa1454450 | 0.813711 | 2.228940e-51 | -8.615850e-47 |
| Msa1045050 | Msa1454680 | 0.818423 | 1.975745e-52 | -8.615850e-47 |
| Msa1045050 | Msa1454950 | 0.806314 | 8.737606e-50 | -8.615850e-47 |
| Msa0271360 | Msa1045050 | 0.813878 | 2.048445e-51 | -8.615850e-47 |
| Msa0309880 | Msa1045050 | 0.826570 | 2.523187e-54 | -8.615850e-47 |
| Msa0511770 | Msa1045050 | 0.801005 | 1.104308e-48 | -8.615850e-47 |
| Msa0887250 | Msa1045050 | 0.801111 | 1.050186e-48 | -8.615850e-47 |
| Msa0890550 | Msa1045050 | 0.815305 | 9.896577e-52 | -8.615850e-47 |
| Msa0923780 | Msa1045050 | 0.800659 | 1.299253e-48 | -8.615850e-47 |
| Msa0944610 | Msa1045050 | 0.819431 | 1.165794e-52 | -8.615850e-47 |
| Msa0973320 | Msa1045050 | 0.803214 | 3.879611e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045050 | MtrunA17_Chr5g0425951 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr4g0030431 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr3g0100121 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr3g0113681 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr2g0327641 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr3g0103351 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr8g0352791 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr3g0103821 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr3g0104031 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr7g0225731 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr3g0103801 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr3g0103881 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr7g0262791 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.62e-51 | 156 |
| Msa1045050 | MtrunA17_Chr4g0072411 | 94.175 | 103 | 6 | 0 | 1 | 103 | 59 | 161 | 3.77e-51 | 158 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1045050 | AT3G53730.1 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 5.00e-51 | 156 |
| Msa1045050 | AT1G07820.2 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 5.00e-51 | 156 |
| Msa1045050 | AT1G07820.1 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 5.00e-51 | 156 |
| Msa1045050 | AT5G59690.1 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 5.00e-51 | 156 |
| Msa1045050 | AT3G46320.1 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 5.00e-51 | 156 |
| Msa1045050 | AT2G28740.1 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 5.00e-51 | 156 |
| Msa1045050 | AT1G07660.1 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 5.00e-51 | 156 |
| Msa1045050 | AT3G45930.1 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 5.00e-51 | 156 |
| Msa1045050 | AT5G59970.1 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 5.00e-51 | 156 |
| Msa1045050 | AT5G59970.2 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 5.00e-51 | 156 |
| Msa1045050 | AT1G07660.2 | 91.549 | 71 | 6 | 0 | 33 | 103 | 16 | 86 | 2.29e-40 | 129 |
Find 33 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGGACTCTTTACGGATT+TGG | 0.296782 | 7_2:-4196601 | Msa1045050:CDS |
| GGTTCTCCGCGACAACATTC+AGG | 0.328775 | 7_2:-4196821 | Msa1045050:CDS |
| GGCAAAGGAGGAAAGGGTTT+AGG | 0.384869 | 7_2:-4196871 | Msa1045050:CDS |
| TGTTGTCGCGGAGAACCTTT+CGG | 0.398530 | 7_2:+4196827 | None:intergenic |
| TCCTTTGCCTCTTCCTGACA+TGG | 0.418595 | 7_2:+4196885 | None:intergenic |
| GGAGGAAAGGGTTTAGGAAA+GGG | 0.429069 | 7_2:-4196865 | Msa1045050:CDS |
| AGGCAGGGAAGGACTCTTTA+CGG | 0.443617 | 7_2:-4196607 | Msa1045050:CDS |
| AAAAGTTTCACAGCCATGTC+AGG | 0.488802 | 7_2:-4196898 | None:intergenic |
| TGTTTACGCTCTCAAGAGGC+AGG | 0.493747 | 7_2:-4196623 | Msa1045050:CDS |
| AGGACTCTTTACGGATTTGG+CGG | 0.517890 | 7_2:-4196598 | Msa1045050:CDS |
| AGGAAGAGGCAAAGGAGGAA+AGG | 0.518412 | 7_2:-4196878 | Msa1045050:CDS |
| GGAAGAGGCAAAGGAGGAAA+GGG | 0.527098 | 7_2:-4196877 | Msa1045050:CDS |
| AGGAGGAAAGGGTTTAGGAA+AGG | 0.528235 | 7_2:-4196866 | Msa1045050:CDS |
| CCATGGCAATAACAGTCTTA+CGG | 0.528943 | 7_2:+4196650 | None:intergenic |
| GGAAAGGGTTTAGGAAAGGG+AGG | 0.536831 | 7_2:-4196862 | Msa1045050:CDS |
| GCCATGTCAGGAAGAGGCAA+AGG | 0.547501 | 7_2:-4196886 | Msa1045050:CDS |
| GTTCTCCGCGACAACATTCA+GGG | 0.553788 | 7_2:-4196820 | Msa1045050:CDS |
| CCGTAAGACTGTTATTGCCA+TGG | 0.561344 | 7_2:-4196650 | Msa1045050:CDS |
| CTTCCGAGACAACGAATCGC+AGG | 0.565616 | 7_2:+4196787 | None:intergenic |
| TTCACAGCCATGTCAGGAAG+AGG | 0.573281 | 7_2:-4196892 | None:intergenic |
| ATGTCAGGAAGAGGCAAAGG+AGG | 0.581057 | 7_2:-4196883 | Msa1045050:CDS |
| ACGCTCTCAAGAGGCAGGGA+AGG | 0.583359 | 7_2:-4196618 | Msa1045050:CDS |
| ACGGCGAGCATGTTTAGTGT+AGG | 0.586831 | 7_2:+4196669 | None:intergenic |
| AAGCCTGCGATTCGTTGTCT+CGG | 0.595434 | 7_2:-4196790 | Msa1045050:CDS |
| AGGAGCAAAACGACACCGAA+AGG | 0.608533 | 7_2:-4196842 | Msa1045050:CDS |
| ATGTTGTTTACGCTCTCAAG+AGG | 0.616421 | 7_2:-4196627 | Msa1045050:CDS |
| CGTTGTCTCGGAAGATGTGG+TGG | 0.617770 | 7_2:-4196778 | Msa1045050:CDS |
| GTTTACGCTCTCAAGAGGCA+GGG | 0.624823 | 7_2:-4196622 | Msa1045050:CDS |
| GAGAGCGTAAACAACATCCA+TGG | 0.638206 | 7_2:+4196633 | None:intergenic |
| ATTCGTTGTCTCGGAAGATG+TGG | 0.639546 | 7_2:-4196781 | Msa1045050:CDS |
| TAATTCCCTGAATGTTGTCG+CGG | 0.640736 | 7_2:+4196815 | None:intergenic |
| TTAATCTATGAAGAAACTCG+TGG | 0.649567 | 7_2:-4196736 | Msa1045050:CDS |
| GGTGGTGTGAAGCGTATCAG+TGG | 0.690731 | 7_2:-4196760 | Msa1045050:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTAATCTATGAAGAAACTCG+TGG | - | chr7_2:4196741-4196760 | Msa1045050:CDS | 30.0% | |
| TAATTCCCTGAATGTTGTCG+CGG | + | chr7_2:4196665-4196684 | None:intergenic | 40.0% | |
| CCATGGCAATAACAGTCTTA+CGG | + | chr7_2:4196830-4196849 | None:intergenic | 40.0% | |
| ! | ATGTTGTTTACGCTCTCAAG+AGG | - | chr7_2:4196850-4196869 | Msa1045050:CDS | 40.0% |
| AGGAGGAAAGGGTTTAGGAA+AGG | - | chr7_2:4196611-4196630 | Msa1045050:CDS | 45.0% | |
| GGAGGAAAGGGTTTAGGAAA+GGG | - | chr7_2:4196612-4196631 | Msa1045050:CDS | 45.0% | |
| ATTCGTTGTCTCGGAAGATG+TGG | - | chr7_2:4196696-4196715 | Msa1045050:CDS | 45.0% | |
| CCGTAAGACTGTTATTGCCA+TGG | - | chr7_2:4196827-4196846 | Msa1045050:CDS | 45.0% | |
| ! | GAGAGCGTAAACAACATCCA+TGG | + | chr7_2:4196847-4196866 | None:intergenic | 45.0% |
| GGAAGGACTCTTTACGGATT+TGG | - | chr7_2:4196876-4196895 | Msa1045050:CDS | 45.0% | |
| AGGACTCTTTACGGATTTGG+CGG | - | chr7_2:4196879-4196898 | Msa1045050:CDS | 45.0% | |
| ATGTCAGGAAGAGGCAAAGG+AGG | - | chr7_2:4196594-4196613 | Msa1045050:CDS | 50.0% | |
| AGGAAGAGGCAAAGGAGGAA+AGG | - | chr7_2:4196599-4196618 | Msa1045050:CDS | 50.0% | |
| GGAAGAGGCAAAGGAGGAAA+GGG | - | chr7_2:4196600-4196619 | Msa1045050:CDS | 50.0% | |
| GGCAAAGGAGGAAAGGGTTT+AGG | - | chr7_2:4196606-4196625 | Msa1045050:CDS | 50.0% | |
| GGAAAGGGTTTAGGAAAGGG+AGG | - | chr7_2:4196615-4196634 | Msa1045050:CDS | 50.0% | |
| AGGAGCAAAACGACACCGAA+AGG | - | chr7_2:4196635-4196654 | Msa1045050:CDS | 50.0% | |
| TGTTGTCGCGGAGAACCTTT+CGG | + | chr7_2:4196653-4196672 | None:intergenic | 50.0% | |
| GTTCTCCGCGACAACATTCA+GGG | - | chr7_2:4196657-4196676 | Msa1045050:CDS | 50.0% | |
| AAGCCTGCGATTCGTTGTCT+CGG | - | chr7_2:4196687-4196706 | Msa1045050:CDS | 50.0% | |
| ACGGCGAGCATGTTTAGTGT+AGG | + | chr7_2:4196811-4196830 | None:intergenic | 50.0% | |
| TGTTTACGCTCTCAAGAGGC+AGG | - | chr7_2:4196854-4196873 | Msa1045050:CDS | 50.0% | |
| GTTTACGCTCTCAAGAGGCA+GGG | - | chr7_2:4196855-4196874 | Msa1045050:CDS | 50.0% | |
| AGGCAGGGAAGGACTCTTTA+CGG | - | chr7_2:4196870-4196889 | Msa1045050:CDS | 50.0% | |
| GGTTCTCCGCGACAACATTC+AGG | - | chr7_2:4196656-4196675 | Msa1045050:CDS | 55.0% | |
| CTTCCGAGACAACGAATCGC+AGG | + | chr7_2:4196693-4196712 | None:intergenic | 55.0% | |
| CGTTGTCTCGGAAGATGTGG+TGG | - | chr7_2:4196699-4196718 | Msa1045050:CDS | 55.0% | |
| GGTGGTGTGAAGCGTATCAG+TGG | - | chr7_2:4196717-4196736 | Msa1045050:CDS | 55.0% | |
| ACGCTCTCAAGAGGCAGGGA+AGG | - | chr7_2:4196859-4196878 | Msa1045050:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 4196594 | 4196905 | 4196594 | ID=Msa1045050;Name=Msa1045050 |
| chr7_2 | mRNA | 4196594 | 4196905 | 4196594 | ID=Msa1045050-mRNA-1;Parent=Msa1045050;Name=Msa1045050-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|103 |
| chr7_2 | exon | 4196594 | 4196905 | 4196594 | ID=Msa1045050-mRNA-1:exon:991;Parent=Msa1045050-mRNA-1 |
| chr7_2 | CDS | 4196594 | 4196905 | 4196594 | ID=Msa1045050-mRNA-1:cds;Parent=Msa1045050-mRNA-1 |
| Gene Sequence |
| Protein sequence |
