AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0309880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0309880 MtrunA17_Chr2g0278531 92.063 63 5 0 30 92 11 73 2.34e-37 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0309880 AT1G25275.3 50.000 70 31 1 25 90 2 71 1.19e-17 71.2
Msa0309880 AT1G25275.1 50.000 70 31 1 25 90 2 71 1.21e-17 70.9
Msa0309880 AT1G25275.2 48.571 70 32 1 25 90 2 71 1.10e-16 68.2

Find 27 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCTTCTGATTGCCTTGA+TGG 0.154913 2_4:-4674450 Msa0309880:CDS
CTCAACCAAGTTTAATTAGA+AGG 0.298295 2_4:+4673743 None:intergenic
CTCTTTGAACGCAGGCAGTT+TGG 0.316423 2_4:+4674424 None:intergenic
ATGTATTGTGATAGCAGAAT+TGG 0.414963 2_4:+4673618 None:intergenic
GTAGTTTCTCAAGATTATTA+TGG 0.419164 2_4:-4674662 Msa0309880:five_prime_UTR
ACAAGCAGCAAACATTGAAT+AGG 0.443291 2_4:+4673773 None:intergenic
GATGTACTAGTCGTAGTTAA+TGG 0.445314 2_4:-4674762 None:intergenic
CGAGACAGTTGACAAGTTGT+TGG 0.451351 2_4:+4673837 None:intergenic
TAGTTGTACATTTATGACTT+TGG 0.453545 2_4:-4674692 Msa0309880:five_prime_UTR
ATGTAGCAAGTAATTGCTTA+AGG 0.470851 2_4:-4673651 Msa0309880:three_prime_UTR
TGCTCGATTAGATGCGGTCC+AGG 0.477134 2_4:-4674182 Msa0309880:intron
TGCAGATTCTATGGTGGAGA+AGG 0.494670 2_4:-4673906 Msa0309880:intron
TAGTGCCTTCTAATTAAACT+TGG 0.497364 2_4:-4673748 Msa0309880:three_prime_UTR
TCAAGGCAATCAGAAGCATC+AGG 0.516390 2_4:+4674453 None:intergenic
GTGACATGCAGCAAGGCTTA+CGG 0.519988 2_4:-4674222 Msa0309880:intron
ATAATAATCTTGAGAAACTA+CGG 0.529331 2_4:+4674664 None:intergenic
GCAGATTCTATGGTGGAGAA+GGG 0.551697 2_4:-4673905 Msa0309880:intron
CAACAACTTGTCAACTGTCT+CGG 0.563127 2_4:-4673836 Msa0309880:three_prime_UTR
AGGCAGTTTGGCAACCATCA+AGG 0.564306 2_4:+4674436 None:intergenic
TTCTATGGTGGAGAAGGGCA+TGG 0.592128 2_4:-4673900 Msa0309880:CDS
ATGCAGCAAGGCTTACGGCA+AGG 0.642528 2_4:-4674217 Msa0309880:intron
CGCAAGTGCTCGATTAGATG+CGG 0.643345 2_4:-4674188 Msa0309880:CDS
AGTATGCATGTACTTCCACA+TGG 0.664590 2_4:-4674716 Msa0309880:five_prime_UTR
CTAACAGTCTCTTTGAACGC+AGG 0.676017 2_4:+4674416 None:intergenic
TAAATGTACAACTATCCATG+TGG 0.677441 2_4:+4674701 None:intergenic
GATGATGAAGAAGACGACGG+TGG 0.786514 2_4:-4674526 Msa0309880:CDS
GAAGATGATGAAGAAGACGA+CGG 0.800011 2_4:-4674529 Msa0309880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAGAAAAGTAATTAAA+AGG + chr2_4:4673959-4673978 None:intergenic 10.0%
!!! CATTAAAATAGTATTATTTT+GGG + chr2_4:4674185-4674204 None:intergenic 10.0%
!!! TCATTAAAATAGTATTATTT+TGG + chr2_4:4674186-4674205 None:intergenic 10.0%
!!! AATAATACTATTTTAATGAA+AGG - chr2_4:4674187-4674206 Msa0309880:CDS 10.0%
!! TATTAAGAAAAGTAATTAAA+AGG + chr2_4:4673959-4673978 None:intergenic 10.0%
!!! CATTAAAATAGTATTATTTT+GGG + chr2_4:4674185-4674204 None:intergenic 10.0%
!!! TCATTAAAATAGTATTATTT+TGG + chr2_4:4674186-4674205 None:intergenic 10.0%
!!! AATAATACTATTTTAATGAA+AGG - chr2_4:4674187-4674206 Msa0309880:CDS 10.0%
!! ATCATAAAAAAAAAACATCA+AGG + chr2_4:4673730-4673749 None:intergenic 15.0%
!! AAATTAATTAAAAAGCTTCA+AGG - chr2_4:4674061-4674080 Msa0309880:intron 15.0%
!!! TTTTTAAGTTAAAAAGCTTA+AGG - chr2_4:4674750-4674769 Msa0309880:five_prime_UTR 15.0%
!! ATCATAAAAAAAAAACATCA+AGG + chr2_4:4673730-4673749 None:intergenic 15.0%
!! AAATTAATTAAAAAGCTTCA+AGG - chr2_4:4674061-4674080 Msa0309880:intron 15.0%
!!! TTTTTAAGTTAAAAAGCTTA+AGG - chr2_4:4674750-4674769 Msa0309880:five_prime_UTR 15.0%
!! ATAATAATCTTGAGAAACTA+CGG + chr2_4:4673668-4673687 None:intergenic 20.0%
!!! ATTTGTGATATTTTCTCAAA+TGG - chr2_4:4673842-4673861 Msa0309880:three_prime_UTR 20.0%
!!! CATTGTCTTCTTTTAATTTA+AGG - chr2_4:4673982-4674001 Msa0309880:intron 20.0%
!!! ATTTAATTTTGCAGATTCTA+TGG - chr2_4:4674414-4674433 Msa0309880:intron 20.0%
!! ATAATAATCTTGAGAAACTA+CGG + chr2_4:4673668-4673687 None:intergenic 20.0%
!!! ATTTGTGATATTTTCTCAAA+TGG - chr2_4:4673842-4673861 Msa0309880:three_prime_UTR 20.0%
!!! CATTGTCTTCTTTTAATTTA+AGG - chr2_4:4673982-4674001 Msa0309880:intron 20.0%
!!! ATTTAATTTTGCAGATTCTA+TGG - chr2_4:4674414-4674433 Msa0309880:intron 20.0%
! TAGTTGTACATTTATGACTT+TGG - chr2_4:4673637-4673656 Msa0309880:three_prime_UTR 25.0%
! GTAGTTTCTCAAGATTATTA+TGG - chr2_4:4673667-4673686 Msa0309880:three_prime_UTR 25.0%
! TAGTTGTACATTTATGACTT+TGG - chr2_4:4673637-4673656 Msa0309880:three_prime_UTR 25.0%
! GTAGTTTCTCAAGATTATTA+TGG - chr2_4:4673667-4673686 Msa0309880:three_prime_UTR 25.0%
TAAATGTACAACTATCCATG+TGG + chr2_4:4673631-4673650 None:intergenic 30.0%
TTTGGGTAATGTTAATTACC+TGG + chr2_4:4674168-4674187 None:intergenic 30.0%
! TAATTTTGCAGATTCTATGG+TGG - chr2_4:4674417-4674436 Msa0309880:intron 30.0%
TAGTGCCTTCTAATTAAACT+TGG - chr2_4:4674581-4674600 Msa0309880:CDS 30.0%
CTCAACCAAGTTTAATTAGA+AGG + chr2_4:4674589-4674608 None:intergenic 30.0%
ATGTAGCAAGTAATTGCTTA+AGG - chr2_4:4674678-4674697 Msa0309880:five_prime_UTR 30.0%
ATGTATTGTGATAGCAGAAT+TGG + chr2_4:4674714-4674733 None:intergenic 30.0%
TAAATGTACAACTATCCATG+TGG + chr2_4:4673631-4673650 None:intergenic 30.0%
TTTGGGTAATGTTAATTACC+TGG + chr2_4:4674168-4674187 None:intergenic 30.0%
! TAATTTTGCAGATTCTATGG+TGG - chr2_4:4674417-4674436 Msa0309880:intron 30.0%
TAGTGCCTTCTAATTAAACT+TGG - chr2_4:4674581-4674600 Msa0309880:CDS 30.0%
CTCAACCAAGTTTAATTAGA+AGG + chr2_4:4674589-4674608 None:intergenic 30.0%
ATGTAGCAAGTAATTGCTTA+AGG - chr2_4:4674678-4674697 Msa0309880:five_prime_UTR 30.0%
ATGTATTGTGATAGCAGAAT+TGG + chr2_4:4674714-4674733 None:intergenic 30.0%
! CTCTTTTCTCTTTGATCGTT+TGG + chr2_4:4673766-4673785 None:intergenic 35.0%
! TTAGTGCTGAAAAGATACTG+AGG - chr2_4:4674235-4674254 Msa0309880:intron 35.0%
CTTGAATGATCGTAAACAAC+AGG - chr2_4:4674271-4674290 Msa0309880:intron 35.0%
ACAAGCAGCAAACATTGAAT+AGG + chr2_4:4674559-4674578 None:intergenic 35.0%
! CTCTTTTCTCTTTGATCGTT+TGG + chr2_4:4673766-4673785 None:intergenic 35.0%
! TTAGTGCTGAAAAGATACTG+AGG - chr2_4:4674235-4674254 Msa0309880:intron 35.0%
CTTGAATGATCGTAAACAAC+AGG - chr2_4:4674271-4674290 Msa0309880:intron 35.0%
ACAAGCAGCAAACATTGAAT+AGG + chr2_4:4674559-4674578 None:intergenic 35.0%
AGTATGCATGTACTTCCACA+TGG - chr2_4:4673613-4673632 Msa0309880:three_prime_UTR 40.0%
GAAGATGATGAAGAAGACGA+CGG - chr2_4:4673800-4673819 Msa0309880:three_prime_UTR 40.0%
TATGTATGTGACATGCAGCA+AGG - chr2_4:4674100-4674119 Msa0309880:intron 40.0%
ATGATCGTAAACAACAGGTC+TGG - chr2_4:4674276-4674295 Msa0309880:intron 40.0%
TGATCGTAAACAACAGGTCT+GGG - chr2_4:4674277-4674296 Msa0309880:intron 40.0%
GTATAAAATTGTCTGAGCCC+AGG + chr2_4:4674356-4674375 None:intergenic 40.0%
CAACAACTTGTCAACTGTCT+CGG - chr2_4:4674493-4674512 Msa0309880:CDS 40.0%
AGTATGCATGTACTTCCACA+TGG - chr2_4:4673613-4673632 Msa0309880:three_prime_UTR 40.0%
GAAGATGATGAAGAAGACGA+CGG - chr2_4:4673800-4673819 Msa0309880:three_prime_UTR 40.0%
TATGTATGTGACATGCAGCA+AGG - chr2_4:4674100-4674119 Msa0309880:intron 40.0%
ATGATCGTAAACAACAGGTC+TGG - chr2_4:4674276-4674295 Msa0309880:intron 40.0%
TGATCGTAAACAACAGGTCT+GGG - chr2_4:4674277-4674296 Msa0309880:intron 40.0%
GTATAAAATTGTCTGAGCCC+AGG + chr2_4:4674356-4674375 None:intergenic 40.0%
CAACAACTTGTCAACTGTCT+CGG - chr2_4:4674493-4674512 Msa0309880:CDS 40.0%
!! TCAAGGCAATCAGAAGCATC+AGG + chr2_4:4673879-4673898 None:intergenic 45.0%
GATGCTTCTGATTGCCTTGA+TGG - chr2_4:4673879-4673898 Msa0309880:exon 45.0%
CTAACAGTCTCTTTGAACGC+AGG + chr2_4:4673916-4673935 None:intergenic 45.0%
! TGCAGATTCTATGGTGGAGA+AGG - chr2_4:4674423-4674442 Msa0309880:CDS 45.0%
! GCAGATTCTATGGTGGAGAA+GGG - chr2_4:4674424-4674443 Msa0309880:CDS 45.0%
CGAGACAGTTGACAAGTTGT+TGG + chr2_4:4674495-4674514 None:intergenic 45.0%
!! TCAAGGCAATCAGAAGCATC+AGG + chr2_4:4673879-4673898 None:intergenic 45.0%
GATGCTTCTGATTGCCTTGA+TGG - chr2_4:4673879-4673898 Msa0309880:exon 45.0%
CTAACAGTCTCTTTGAACGC+AGG + chr2_4:4673916-4673935 None:intergenic 45.0%
! TGCAGATTCTATGGTGGAGA+AGG - chr2_4:4674423-4674442 Msa0309880:CDS 45.0%
! GCAGATTCTATGGTGGAGAA+GGG - chr2_4:4674424-4674443 Msa0309880:CDS 45.0%
CGAGACAGTTGACAAGTTGT+TGG + chr2_4:4674495-4674514 None:intergenic 45.0%
GATGATGAAGAAGACGACGG+TGG - chr2_4:4673803-4673822 Msa0309880:three_prime_UTR 50.0%
AGGCAGTTTGGCAACCATCA+AGG + chr2_4:4673896-4673915 None:intergenic 50.0%
CTCTTTGAACGCAGGCAGTT+TGG + chr2_4:4673908-4673927 None:intergenic 50.0%
GTGACATGCAGCAAGGCTTA+CGG - chr2_4:4674107-4674126 Msa0309880:intron 50.0%
CGCAAGTGCTCGATTAGATG+CGG - chr2_4:4674141-4674160 Msa0309880:intron 50.0%
TCGTAAACAACAGGTCTGGG+TGG - chr2_4:4674280-4674299 Msa0309880:intron 50.0%
! ACGCTAGTCTCACACACTAG+AGG - chr2_4:4674317-4674336 Msa0309880:intron 50.0%
! TTCTATGGTGGAGAAGGGCA+TGG - chr2_4:4674429-4674448 Msa0309880:CDS 50.0%
TGTGCGCCATTAACTACGAC+AGG + chr2_4:4673576-4673595 None:intergenic 50.0%
GATGATGAAGAAGACGACGG+TGG - chr2_4:4673803-4673822 Msa0309880:three_prime_UTR 50.0%
AGGCAGTTTGGCAACCATCA+AGG + chr2_4:4673896-4673915 None:intergenic 50.0%
CTCTTTGAACGCAGGCAGTT+TGG + chr2_4:4673908-4673927 None:intergenic 50.0%
GTGACATGCAGCAAGGCTTA+CGG - chr2_4:4674107-4674126 Msa0309880:intron 50.0%
CGCAAGTGCTCGATTAGATG+CGG - chr2_4:4674141-4674160 Msa0309880:intron 50.0%
TCGTAAACAACAGGTCTGGG+TGG - chr2_4:4674280-4674299 Msa0309880:intron 50.0%
! ACGCTAGTCTCACACACTAG+AGG - chr2_4:4674317-4674336 Msa0309880:intron 50.0%
! TTCTATGGTGGAGAAGGGCA+TGG - chr2_4:4674429-4674448 Msa0309880:CDS 50.0%
ATGCAGCAAGGCTTACGGCA+AGG - chr2_4:4674112-4674131 Msa0309880:intron 55.0%
TGCTCGATTAGATGCGGTCC+AGG - chr2_4:4674147-4674166 Msa0309880:intron 55.0%
ACAGGTCTGGGTGGTGTAGT+CGG - chr2_4:4674289-4674308 Msa0309880:intron 55.0%
TCTGAGCCCAGGTTCGAACT+GGG + chr2_4:4674345-4674364 None:intergenic 55.0%
ATGCAGCAAGGCTTACGGCA+AGG - chr2_4:4674112-4674131 Msa0309880:intron 55.0%
TGCTCGATTAGATGCGGTCC+AGG - chr2_4:4674147-4674166 Msa0309880:intron 55.0%
ACAGGTCTGGGTGGTGTAGT+CGG - chr2_4:4674289-4674308 Msa0309880:intron 55.0%
TCTGAGCCCAGGTTCGAACT+GGG + chr2_4:4674345-4674364 None:intergenic 55.0%
AGAGGTCCCCAGTTCGAACC+TGG - chr2_4:4674335-4674354 Msa0309880:intron 60.0%
GAGGTCCCCAGTTCGAACCT+GGG - chr2_4:4674336-4674355 Msa0309880:intron 60.0%
CTGAGCCCAGGTTCGAACTG+GGG + chr2_4:4674344-4674363 None:intergenic 60.0%
GTCTGAGCCCAGGTTCGAAC+TGG + chr2_4:4674346-4674365 None:intergenic 60.0%
AGAGGTCCCCAGTTCGAACC+TGG - chr2_4:4674335-4674354 Msa0309880:intron 60.0%
GAGGTCCCCAGTTCGAACCT+GGG - chr2_4:4674336-4674355 Msa0309880:intron 60.0%
CTGAGCCCAGGTTCGAACTG+GGG + chr2_4:4674344-4674363 None:intergenic 60.0%
GTCTGAGCCCAGGTTCGAAC+TGG + chr2_4:4674346-4674365 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 4673576 4674775 4673576 ID=Msa0309880;Name=Msa0309880
chr2_4 mRNA 4673576 4674775 4673576 ID=Msa0309880-mRNA-1;Parent=Msa0309880;Name=Msa0309880-mRNA-1;_AED=0.00;_eAED=0.00;_QI=156|1|1|1|1|1|3|319|92
chr2_4 exon 4673576 4673923 4673576 ID=Msa0309880-mRNA-1:exon:1589;Parent=Msa0309880-mRNA-1
chr2_4 exon 4674183 4674233 4674183 ID=Msa0309880-mRNA-1:exon:1588;Parent=Msa0309880-mRNA-1
chr2_4 exon 4674421 4674775 4674421 ID=Msa0309880-mRNA-1:exon:1587;Parent=Msa0309880-mRNA-1
chr2_4 five_prime_UTR 4674620 4674775 4674620 ID=Msa0309880-mRNA-1:five_prime_utr;Parent=Msa0309880-mRNA-1
chr2_4 CDS 4674421 4674619 4674421 ID=Msa0309880-mRNA-1:cds;Parent=Msa0309880-mRNA-1
chr2_4 CDS 4674183 4674233 4674183 ID=Msa0309880-mRNA-1:cds;Parent=Msa0309880-mRNA-1
chr2_4 CDS 4673895 4673923 4673895 ID=Msa0309880-mRNA-1:cds;Parent=Msa0309880-mRNA-1
chr2_4 three_prime_UTR 4673576 4673894 4673576 ID=Msa0309880-mRNA-1:three_prime_utr;Parent=Msa0309880-mRNA-1
Gene Sequence

>Msa0309880

ATGTTTTTTTTTTATGATTCAAGAGAATCTACCAAACGATCAAAGAGAAAAGAGAAAGTAAAGTTTGTGAAGATGATGAAGAAGACGACGGTGGTTGCTATGATGCTTTTATTTGTGATATTTTCTCAAATGGATAGCGTTGTTCCTGATGCTTCTGATTGCCTTGATGGTTGCCAAACTGCCTGCGTTCAAAGAGACTCAAGGCTTACGGCAAGGTGTGAGCGCAAGTGCTCGATTAGATGCGGTCCAGATTCTATGGTGGAGAAGGGCATGGATTGA

Protein sequence

>Msa0309880

MFFFYDSRESTKRSKRKEKVKFVKMMKKTTVVAMMLLFVIFSQMDSVVPDASDCLDGCQTACVQRDSRLTARCERKCSIRCGPDSMVEKGMD*