AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1344130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1344130 MtrunA17_Chr1g0206761 49.057 106 17 3 1 72 1 103 7.79e-16 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTAATGGGTTCTGTTCA+AGG 0.346498 tig0006790:-41697 None:intergenic
AGTTCTCAAGTGGAGGAAAA+AGG 0.353703 tig0006790:-41510 Msa1344130:CDS
GGTAGTAAAGGGAGTGTTGC+TGG 0.355967 tig0006790:-41444 Msa1344130:CDS
AAAACCATTCATCAGATAAC+TGG 0.401419 tig0006790:-41408 Msa1344130:CDS
TTTGGAAGAAACGGCAGGTT+CGG 0.430422 tig0006790:-41665 Msa1344130:intron
AGAGTCATGGTGGTGGTGGC+AGG 0.445844 tig0006790:-41551 Msa1344130:CDS
TGTTCAAGGTGATCGAGCTT+TGG 0.459674 tig0006790:-41683 Msa1344130:CDS
CTAACCAGTTATCTGATGAA+TGG 0.462802 tig0006790:+41404 Msa1344130:intergenic
CCTGCAGGCACTGGTAGTAA+AGG 0.468442 tig0006790:-41456 Msa1344130:CDS
CTGCAGGCACTGGTAGTAAA+GGG 0.481427 tig0006790:-41455 Msa1344130:CDS
GACAATTTCTAGTTCTCAAG+TGG 0.492881 tig0006790:-41520 Msa1344130:CDS
CCTTTACTACCAGTGCCTGC+AGG 0.505585 tig0006790:+41456 Msa1344130:intergenic
AAGGAGAGTCATGGTGGTGG+TGG 0.519066 tig0006790:-41555 Msa1344130:CDS
TGATCGAGCTTTGGAAGAAA+CGG 0.531100 tig0006790:-41674 Msa1344130:CDS
CGAGCTTTGGAAGAAACGGC+AGG 0.564383 tig0006790:-41670 Msa1344130:CDS
TGGAAGGAGAGTCATGGTGG+TGG 0.582142 tig0006790:-41558 Msa1344130:intron
AATTTCTAGTTCTCAAGTGG+AGG 0.602033 tig0006790:-41517 Msa1344130:CDS
CCGTGGAAGGAGAGTCATGG+TGG 0.660355 tig0006790:-41561 Msa1344130:intron
TGGCCGTGGAAGGAGAGTCA+TGG 0.660402 tig0006790:-41564 Msa1344130:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATAATAACGAAAATTAAC+AGG + tig0006790:41488-41507 Msa1344130:intergenic 20.0%
AAAACCATTCATCAGATAAC+TGG - tig0006790:41687-41706 Msa1344130:CDS 30.0%
!! GACAATTTCTAGTTCTCAAG+TGG - tig0006790:41575-41594 Msa1344130:intron 35.0%
!! AATTTCTAGTTCTCAAGTGG+AGG - tig0006790:41578-41597 Msa1344130:intron 35.0%
CTAACCAGTTATCTGATGAA+TGG + tig0006790:41694-41713 Msa1344130:intergenic 35.0%
!! TGATCGAGCTTTGGAAGAAA+CGG - tig0006790:41421-41440 Msa1344130:CDS 40.0%
AACGGTACTCTTTGTCATTC+TGG + tig0006790:41510-41529 Msa1344130:intergenic 40.0%
!! AATGACAAAGAGTACCGTTG+TGG - tig0006790:41511-41530 Msa1344130:CDS 40.0%
AGTTCTCAAGTGGAGGAAAA+AGG - tig0006790:41585-41604 Msa1344130:intron 40.0%
GACAAGAAAAACAAACCTGC+AGG - tig0006790:41624-41643 Msa1344130:intron 40.0%
TGTTCAAGGTGATCGAGCTT+TGG - tig0006790:41412-41431 Msa1344130:CDS 45.0%
TTTGGAAGAAACGGCAGGTT+CGG - tig0006790:41430-41449 Msa1344130:CDS 45.0%
AAAAACAAACCTGCAGGCAC+TGG - tig0006790:41630-41649 Msa1344130:intron 45.0%
!! CTGCAGGCACTGGTAGTAAA+GGG - tig0006790:41640-41659 Msa1344130:intron 50.0%
!! GGTAGTAAAGGGAGTGTTGC+TGG - tig0006790:41651-41670 Msa1344130:intron 50.0%
!! CGAGCTTTGGAAGAAACGGC+AGG - tig0006790:41425-41444 Msa1344130:CDS 55.0%
!! AAAGAGTACCGTTGTGGCCG+TGG - tig0006790:41517-41536 Msa1344130:CDS 55.0%
CCACCATGACTCTCCTTCCA+CGG + tig0006790:41537-41556 Msa1344130:intergenic 55.0%
TGGAAGGAGAGTCATGGTGG+TGG - tig0006790:41537-41556 Msa1344130:CDS 55.0%
!! AAGGAGAGTCATGGTGGTGG+TGG - tig0006790:41540-41559 Msa1344130:CDS 55.0%
CCTTTACTACCAGTGCCTGC+AGG + tig0006790:41642-41661 Msa1344130:intergenic 55.0%
!! CCTGCAGGCACTGGTAGTAA+AGG - tig0006790:41639-41658 Msa1344130:intron 55.0%
AGTACCGTTGTGGCCGTGGA+AGG - tig0006790:41521-41540 Msa1344130:CDS 60.0%
CTCTCCTTCCACGGCCACAA+CGG + tig0006790:41528-41547 Msa1344130:intergenic 60.0%
TGGCCGTGGAAGGAGAGTCA+TGG - tig0006790:41531-41550 Msa1344130:CDS 60.0%
CCGTGGAAGGAGAGTCATGG+TGG - tig0006790:41534-41553 Msa1344130:CDS 60.0%
!! AGAGTCATGGTGGTGGTGGC+AGG - tig0006790:41544-41563 Msa1344130:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0006790 gene 41404 41713 41404 ID=Msa1344130;Name=Msa1344130
tig0006790 mRNA 41404 41713 41404 ID=Msa1344130-mRNA-1;Parent=Msa1344130;Name=Msa1344130-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0|0|1|1|1|2|0|72
tig0006790 exon 41666 41713 41666 ID=Msa1344130-mRNA-1:exon:4379;Parent=Msa1344130-mRNA-1
tig0006790 exon 41404 41574 41404 ID=Msa1344130-mRNA-1:exon:4378;Parent=Msa1344130-mRNA-1
tig0006790 CDS 41666 41713 41666 ID=Msa1344130-mRNA-1:cds;Parent=Msa1344130-mRNA-1
tig0006790 CDS 41404 41574 41404 ID=Msa1344130-mRNA-1:cds;Parent=Msa1344130-mRNA-1
Gene Sequence

>Msa1344130

ATGGGTTCTGTTCAAGGTGATCGAGCTTTGGAAGAAACGGCAGGTTCGGAGAGTCATGGTGGTGGTGGCAGGAAAAGAAAGACAATTTCTAGTTCTCAAGTGGAGGAAAAAGGTAGTTTTGTAAAGAAAGACAAGAAAAACAAACCTGCAGGCACTGGTAGTAAAGGGAGTGTTGCTGGAAAGAGTAAACATAAAACCATTCATCAGATAACTGGTTAG

Protein sequence

>Msa1344130

MGSVQGDRALEETAGSESHGGGGRKRKTISSSQVEEKGSFVKKDKKNKPAGTGSKGSVAGKSKHKTIHQITG*