AlfalfaGEDB | Msa1305050

M. sativa cultivar zhongmu-4 / Msa1305050

KEGG: K02883
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0005886 GO:0005911 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015934 GO:0016020 GO:0019538 GO:0022625 GO:0022626 GO:0030054 GO:0031090 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0055044 GO:0071704 GO:0071944 GO:0097159 GO:0098588 GO:0098805 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF17135.7

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1305050	NP_001365649.1	84.475	219	2	1	1	219	1	187	6.70e-123	356

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1305050	sp\|O65729\|RL18_CICAR	73.611	216	24	1	4	219	1	183	1.21e-106	307

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1305050	B7FMF5	84.475	219	2	1	1	219	1	187	3.20e-123	356

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002750	Msa1305050	0.849795	2.571299e-60	-8.615850e-47
Msa0002790	Msa1305050	0.860220	2.403467e-63	-8.615850e-47
Msa0003780	Msa1305050	0.825747	3.961019e-54	-8.615850e-47
Msa0006910	Msa1305050	0.811440	6.992509e-51	-8.615850e-47
Msa0022970	Msa1305050	0.827234	1.750868e-54	-8.615850e-47
Msa0027260	Msa1305050	0.839505	1.522394e-57	-8.615850e-47
Msa0040690	Msa1305050	0.819404	1.182343e-52	-8.615850e-47
Msa0042280	Msa1305050	0.871499	6.524323e-67	-8.615850e-47
Msa0045730	Msa1305050	0.828185	1.034687e-54	-8.615850e-47
Msa0047160	Msa1305050	0.827137	1.847265e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1305050	MtrunA17_Chr7g0272381	84.475	219	2	1	1	219	1	187	6.16e-127	356
Msa1305050	MtrunA17_Chr1g0192271	73.973	219	25	1	1	219	1	187	5.26e-111	315
Msa1305050	MtrunA17_Chr7g0269451	74.091	220	24	2	1	219	1	188	1.30e-108	310

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1305050	AT5G27850.1	69.863	219	34	1	1	219	1	187	2.54e-105	301
Msa1305050	AT3G05590.1	69.406	219	35	1	1	219	1	187	1.15e-104	300
Msa1305050	AT2G47570.1	64.220	218	45	2	3	219	1	186	1.41e-93	272
Msa1305050	AT3G05590.3	65.060	166	26	1	54	219	1	134	2.19e-71	214
Msa1305050	AT3G05590.2	65.060	166	26	1	54	219	1	134	2.19e-71	214
Msa1305050	AT5G27850.2	64.458	166	27	1	54	219	1	134	4.38e-70	210

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Find 90 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTAATACCCATTTAT+TGG	0.197066	8_3:+68342030	None:intergenic
AAACACTTTGGTCGTGCTCC+TGG	0.468460	8_3:-68342307	Msa1305050:CDS
AAAGGGAAGGAAACAAGAGA+AGG	0.607313	8_3:-68342248	Msa1305050:CDS
AAAGTTGATCGAGTACATGA+AGG	0.551641	8_3:-68343079	Msa1305050:CDS
AAATGGAATGGACCTTGACG+AGG	0.641410	8_3:+68344479	None:intergenic
AACTTTGACAGAGAAATTGG+AGG	0.595797	8_3:+68343096	None:intergenic
AAGAGAAGGCCAGAGGAAAG+AGG	0.507573	8_3:-68342234	Msa1305050:CDS
AAGAGGCCCAAAGAATGCTA+GGG	0.636036	8_3:-68342338	Msa1305050:CDS
AAGCCTTATGTAAGATCAAA+GGG	0.467688	8_3:-68342265	Msa1305050:CDS
AAGTTGATCGAGTACATGAA+GGG	0.557741	8_3:-68343078	Msa1305050:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGGTAGATATCATTGGATTT+AGG	+	chr8_3:68342125-68342144	None:intergenic	30.0%
TTGGAACTAATTGTATTCCT+TGG	+	chr8_3:68342323-68342342	None:intergenic	30.0%
AATTATAGTCCTAGACCTTA+CGG	+	chr8_3:68342495-68342514	None:intergenic	30.0%
ATAGCTTAAAACACACACTA+AGG	+	chr8_3:68342740-68342759	None:intergenic	30.0%
ATGCATATACTTAGTCCTAA+TGG	+	chr8_3:68342871-68342890	None:intergenic	30.0%
GTTGGAACTAATTATATTCC+TGG	+	chr8_3:68343019-68343038	None:intergenic	30.0%
GTACAATTGATTATATCCAG+AGG	+	chr8_3:68343057-68343076	None:intergenic	30.0%
TGGTTAAAAGTAACATCACT+GGG	+	chr8_3:68343228-68343247	None:intergenic	30.0%
ATGGTTAAAAGTAACATCAC+TGG	+	chr8_3:68343229-68343248	None:intergenic	30.0%
ATCAACTTTGACAGAGAAAT+TGG	+	chr8_3:68343540-68343559	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	68341897	68344755	68341897	ID=Msa1305050;Name=Msa1305050
chr8_3	mRNA	68341897	68344755	68341897	ID=Msa1305050-mRNA-1;Parent=Msa1305050;Name=Msa1305050-mRNA-1;_AED=0.29;_eAED=0.29;_QI=67\|0.6\|0.83\|0.83\|0.8\|0.83\|6\|313\|219
chr8_3	exon	68341897	68342368	68341897	ID=Msa1305050-mRNA-1:exon:21172;Parent=Msa1305050-mRNA-1
chr8_3	exon	68342461	68342556	68342461	ID=Msa1305050-mRNA-1:exon:21171;Parent=Msa1305050-mRNA-1
chr8_3	exon	68342781	68342963	68342781	ID=Msa1305050-mRNA-1:exon:21170;Parent=Msa1305050-mRNA-1
chr8_3	exon	68343074	68343202	68343074	ID=Msa1305050-mRNA-1:exon:21169;Parent=Msa1305050-mRNA-1
chr8_3	exon	68344492	68344581	68344492	ID=Msa1305050-mRNA-1:exon:21168;Parent=Msa1305050-mRNA-1
chr8_3	exon	68344686	68344755	68344686	ID=Msa1305050-mRNA-1:exon:21167;Parent=Msa1305050-mRNA-1
chr8_3	five_prime_UTR	68344689	68344755	68344689	ID=Msa1305050-mRNA-1:five_prime_utr;Parent=Msa1305050-mRNA-1
chr8_3	CDS	68344686	68344688	68344686	ID=Msa1305050-mRNA-1:cds;Parent=Msa1305050-mRNA-1
chr8_3	CDS	68344492	68344581	68344492	ID=Msa1305050-mRNA-1:cds;Parent=Msa1305050-mRNA-1
chr8_3	CDS	68343074	68343202	68343074	ID=Msa1305050-mRNA-1:cds;Parent=Msa1305050-mRNA-1
chr8_3	CDS	68342781	68342963	68342781	ID=Msa1305050-mRNA-1:cds;Parent=Msa1305050-mRNA-1
chr8_3	CDS	68342461	68342556	68342461	ID=Msa1305050-mRNA-1:cds;Parent=Msa1305050-mRNA-1
chr8_3	CDS	68342210	68342368	68342210	ID=Msa1305050-mRNA-1:cds;Parent=Msa1305050-mRNA-1
chr8_3	three_prime_UTR	68341897	68342209	68341897	ID=Msa1305050-mRNA-1:three_prime_utr;Parent=Msa1305050-mRNA-1

Gene Sequence

>Msa1305050

ATGGGGATCGATCTCAAAGCTGGGGGTAAGAGCAAAAACACCAAACGAACCGAACCTAAATCCAATGATATCTACCTCAAGCTCCTCGTCAAGCTATATCGCTTCCTTGTTAGAAGGACTGACAGCAATTTCAATGCGGTAATACTCAAGCGCTTGTTCATGAGCAAGGTCAACCGACCTCCAATTTCTCTGTCAAAGTTGATCGAGTACATGAAGGGGAAGGACGGTAAAATTGGTGTCGTTGTGGGGGCTGTAACAGATGATATCAGGGTAAATGAAGTCCCAGTATTAAAAATTGCTGCCCTTAGGTTCACAGAGACGGCACGTGCAAGAATAGAGAAGGCGGGTGGTGAATGCCTAACATTTGATCAACTTGCCCTTAGAGCTCCCCTTGGACAGAACACGTATTGTTTTGTTATATTAGCTCTTCCTGTGAGTAGTTGTGATGGGATAGCCTTGGTTATGTTGAGCTTACCAAGTTTTGTTATATTAGCTCTTCCTGTCCTCCTAAGAGGCCCAAAGAATGCTAGGGAAGCAGTAAAACACTTTGGTCGTGCTCCTGGTGTGCCACACAGCCATTCCAAGCCTTATGTAAGATCAAAGGGAAGGAAACAAGAGAAGGCCAGAGGAAAGAGGAAGAGTAGAGGATTTAGGGTTTGA

Protein sequence

>Msa1305050

MGIDLKAGGKSKNTKRTEPKSNDIYLKLLVKLYRFLVRRTDSNFNAVILKRLFMSKVNRPPISLSKLIEYMKGKDGKIGVVVGAVTDDIRVNEVPVLKIAALRFTETARARIEKAGGECLTFDQLALRAPLGQNTYCFVILALPVSSCDGIALVMLSLPSFVILALPVLLRGPKNAREAVKHFGRAPGVPHSHSKPYVRSKGRKQEKARGKRKSRGFRV*