Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1186980 | XP_003627978.1 | 98.913 | 92 | 1 | 0 | 1 | 92 | 29 | 120 | 4.80e-63 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1186980 | sp|Q9SGA6|RS191_ARATH | 82.609 | 92 | 16 | 0 | 1 | 92 | 29 | 120 | 1.45e-57 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1186980 | B7FHE5 | 98.913 | 92 | 1 | 0 | 1 | 92 | 29 | 120 | 2.29e-63 | 197 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0045730 | Msa1186980 | 0.844883 | 5.737555e-59 | -8.615850e-47 |
| Msa0116130 | Msa1186980 | 0.866236 | 3.305493e-65 | -8.615850e-47 |
| Msa0116670 | Msa1186980 | 0.820254 | 7.558480e-53 | -8.615850e-47 |
| Msa0118710 | Msa1186980 | 0.803654 | 3.143706e-49 | -8.615850e-47 |
| Msa0622750 | Msa1186980 | 0.814301 | 1.652192e-51 | -8.615850e-47 |
| Msa0623200 | Msa1186980 | 0.847457 | 1.142524e-59 | -8.615850e-47 |
| Msa0712610 | Msa1186980 | 0.818175 | 2.248531e-52 | -8.615850e-47 |
| Msa0717380 | Msa1186980 | 0.860043 | 2.719383e-63 | -8.615850e-47 |
| Msa0729200 | Msa1186980 | 0.823297 | 1.494326e-53 | -8.615850e-47 |
| Msa0769590 | Msa1186980 | 0.822141 | 2.776644e-53 | -8.615850e-47 |
| Msa0799830 | Msa1186980 | 0.821063 | 4.927989e-53 | -8.615850e-47 |
| Msa0806880 | Msa1186980 | 0.814919 | 1.205501e-51 | -8.615850e-47 |
| Msa0827380 | Msa1186980 | 0.823155 | 1.613142e-53 | -8.615850e-47 |
| Msa0133540 | Msa1186980 | 0.809991 | 1.439221e-50 | -8.615850e-47 |
| Msa0173530 | Msa1186980 | 0.816270 | 6.028598e-52 | -8.615850e-47 |
| Msa0181490 | Msa1186980 | 0.816357 | 5.764725e-52 | -8.615850e-47 |
| Msa0218830 | Msa1186980 | 0.822507 | 2.282637e-53 | -8.615850e-47 |
| Msa1048690 | Msa1186980 | 0.801733 | 7.835097e-49 | -8.615850e-47 |
| Msa0262480 | Msa1186980 | 0.813055 | 3.106354e-51 | -8.615850e-47 |
| Msa0297830 | Msa1186980 | 0.808156 | 3.557334e-50 | -8.615850e-47 |
| Msa0306690 | Msa1186980 | 0.830059 | 3.634443e-55 | -8.615850e-47 |
| Msa0335840 | Msa1186980 | 0.805754 | 1.145885e-49 | -8.615850e-47 |
| Msa0337670 | Msa1186980 | 0.831118 | 2.000633e-55 | -8.615850e-47 |
| Msa0348830 | Msa1186980 | 0.802444 | 5.593871e-49 | -8.615850e-47 |
| Msa1186980 | Msa1349570 | 0.808100 | 3.655332e-50 | -8.615850e-47 |
| Msa1186980 | Msa1356790 | 0.803041 | 4.211993e-49 | -8.615850e-47 |
| Msa1186980 | Msa1369190 | 0.809578 | 1.765920e-50 | -8.615850e-47 |
| Msa1186980 | Msa1374240 | 0.816939 | 4.269374e-52 | -8.615850e-47 |
| Msa1186980 | Msa1403910 | 0.814026 | 1.899660e-51 | -8.615850e-47 |
| Msa1186980 | Msa1406080 | 0.825976 | 3.494521e-54 | -8.615850e-47 |
| Msa1186980 | Msa1413500 | 0.815684 | 8.151213e-52 | -8.615850e-47 |
| Msa1186980 | Msa1426370 | 0.817991 | 2.474436e-52 | -8.615850e-47 |
| Msa1186980 | Msa1426490 | 0.842870 | 1.985325e-58 | -8.615850e-47 |
| Msa1186980 | Msa1455950 | 0.804366 | 2.236466e-49 | -8.615850e-47 |
| Msa0596370 | Msa1186980 | 0.805716 | 1.167014e-49 | -8.615850e-47 |
| Msa0876170 | Msa1186980 | 0.805442 | 1.332555e-49 | -8.615850e-47 |
| Msa0945480 | Msa1186980 | 0.810818 | 9.539234e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1186980 | MtrunA17_Chr8g0353881 | 98.913 | 92 | 1 | 0 | 1 | 92 | 29 | 120 | 4.41e-67 | 197 |
| Msa1186980 | MtrunA17_Chr3g0081741 | 91.304 | 92 | 8 | 0 | 1 | 92 | 29 | 120 | 8.17e-64 | 189 |
| Msa1186980 | MtrunA17_Chr3g0081771 | 78.571 | 56 | 12 | 0 | 30 | 85 | 8 | 63 | 1.61e-28 | 98.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1186980 | AT3G02080.1 | 82.609 | 92 | 16 | 0 | 1 | 92 | 29 | 120 | 1.47e-58 | 176 |
| Msa1186980 | AT5G15520.1 | 81.522 | 92 | 17 | 0 | 1 | 92 | 29 | 120 | 2.00e-57 | 173 |
| Msa1186980 | AT5G61170.1 | 79.348 | 92 | 19 | 0 | 1 | 92 | 29 | 120 | 2.60e-57 | 173 |
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCATTCCAAAGGATTTA+TGG | 0.171916 | 8_1:-26200178 | Msa1186980:CDS |
| AACTGCAAAATTCAAAGAAT+TGG | 0.319374 | 8_1:-26200403 | Msa1186980:CDS |
| ATAATACCAATCAGGATCAT+AGG | 0.347366 | 8_1:+26200377 | None:intergenic |
| GGCTCCCACCATAAATCCTT+TGG | 0.389483 | 8_1:+26200170 | None:intergenic |
| ATCTACATAAGAGGCGGTCT+TGG | 0.403127 | 8_1:-26200205 | Msa1186980:CDS |
| GTACTCAATTTCAGCTTCCA+TGG | 0.471471 | 8_1:-26200236 | Msa1186980:intron |
| TGGCTCCCTATGATCCTGAT+TGG | 0.474063 | 8_1:-26200383 | Msa1186980:CDS |
| GCTCTAACATAATACCAATC+AGG | 0.489642 | 8_1:+26200369 | None:intergenic |
| TTGGTGTTGGTTCATTCCAA+AGG | 0.500013 | 8_1:-26200186 | Msa1186980:CDS |
| TATGTAGATCTTCCTTGCCA+TGG | 0.510578 | 8_1:+26200219 | None:intergenic |
| GAGGGCGACTTCCATTCCTC+TGG | 0.516528 | 8_1:+26200149 | None:intergenic |
| ATAAGAGGCGGTCTTGGTGT+TGG | 0.519137 | 8_1:-26200199 | Msa1186980:CDS |
| TAATACCAATCAGGATCATA+GGG | 0.537599 | 8_1:+26200378 | None:intergenic |
| CAATTTCAGCTTCCATGGCA+AGG | 0.544710 | 8_1:-26200231 | Msa1186980:intron |
| GCAAGGAAGATCTACATAAG+AGG | 0.545628 | 8_1:-26200214 | Msa1186980:CDS |
| CATTCCAAAGGATTTATGGT+GGG | 0.572274 | 8_1:-26200174 | Msa1186980:CDS |
| GGATTTATGGTGGGAGCCAG+AGG | 0.572742 | 8_1:-26200165 | Msa1186980:CDS |
| GATTGGTATTATGTTAGAGC+TGG | 0.573765 | 8_1:-26200366 | Msa1186980:intron |
| GCGTCACATTCTTCAACAGT+TGG | 0.581832 | 8_1:-26200095 | Msa1186980:CDS |
| GAACATGAACCTCATCGAGA+TGG | 0.583253 | 8_1:-26200071 | Msa1186980:CDS |
| TCATCGAGATGGATACCAAA+GGG | 0.584221 | 8_1:-26200060 | Msa1186980:CDS |
| TATGGTGGGAGCCAGAGGAA+TGG | 0.616028 | 8_1:-26200160 | Msa1186980:CDS |
| TCATTCCAAAGGATTTATGG+TGG | 0.619819 | 8_1:-26200175 | Msa1186980:CDS |
| TTGACAAGATCAGTCCACTC+AGG | 0.623178 | 8_1:+26200426 | None:intergenic |
| AATAGTTGGAGCTGCCTGAG+TGG | 0.627148 | 8_1:-26200440 | None:intergenic |
| CTCATCGAGATGGATACCAA+AGG | 0.653842 | 8_1:-26200061 | Msa1186980:CDS |
| CTTTGGTATCCATCTCGATG+AGG | 0.659041 | 8_1:+26200062 | None:intergenic |
| AGGAAGATCTACATAAGAGG+CGG | 0.678529 | 8_1:-26200211 | Msa1186980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTGCAAAATTCAAAGAAT+TGG | - | chr8_1:26200089-26200108 | Msa1186980:CDS | 25.0% |
| ! | AACATGATACTTCTAATTCT+TGG | - | chr8_1:26200192-26200211 | Msa1186980:CDS | 25.0% |
| TAATACCAATCAGGATCATA+GGG | + | chr8_1:26200117-26200136 | None:intergenic | 30.0% | |
| ATAATACCAATCAGGATCAT+AGG | + | chr8_1:26200118-26200137 | None:intergenic | 30.0% | |
| GCTCTAACATAATACCAATC+AGG | + | chr8_1:26200126-26200145 | None:intergenic | 35.0% | |
| GATTGGTATTATGTTAGAGC+TGG | - | chr8_1:26200126-26200145 | Msa1186980:CDS | 35.0% | |
| GGTTCATTCCAAAGGATTTA+TGG | - | chr8_1:26200314-26200333 | Msa1186980:intron | 35.0% | |
| TCATTCCAAAGGATTTATGG+TGG | - | chr8_1:26200317-26200336 | Msa1186980:intron | 35.0% | |
| CATTCCAAAGGATTTATGGT+GGG | - | chr8_1:26200318-26200337 | Msa1186980:intron | 35.0% | |
| ! | TCTTGGTATTTTGTTGCTGC+TGG | - | chr8_1:26200209-26200228 | Msa1186980:CDS | 40.0% |
| GTACTCAATTTCAGCTTCCA+TGG | - | chr8_1:26200256-26200275 | Msa1186980:intron | 40.0% | |
| TATGTAGATCTTCCTTGCCA+TGG | + | chr8_1:26200276-26200295 | None:intergenic | 40.0% | |
| GCAAGGAAGATCTACATAAG+AGG | - | chr8_1:26200278-26200297 | Msa1186980:intron | 40.0% | |
| AGGAAGATCTACATAAGAGG+CGG | - | chr8_1:26200281-26200300 | Msa1186980:intron | 40.0% | |
| !! | TTGGTGTTGGTTCATTCCAA+AGG | - | chr8_1:26200306-26200325 | Msa1186980:intron | 40.0% |
| TGCTCTTGCAAAAATGTGGA+GGG | + | chr8_1:26200364-26200383 | None:intergenic | 40.0% | |
| TCATCGAGATGGATACCAAA+GGG | - | chr8_1:26200432-26200451 | Msa1186980:CDS | 40.0% | |
| TTGACAAGATCAGTCCACTC+AGG | + | chr8_1:26200069-26200088 | None:intergenic | 45.0% | |
| CAATTTCAGCTTCCATGGCA+AGG | - | chr8_1:26200261-26200280 | Msa1186980:intron | 45.0% | |
| ATCTACATAAGAGGCGGTCT+TGG | - | chr8_1:26200287-26200306 | Msa1186980:intron | 45.0% | |
| CTGCTCTTGCAAAAATGTGG+AGG | + | chr8_1:26200365-26200384 | None:intergenic | 45.0% | |
| CCACTGCTCTTGCAAAAATG+TGG | + | chr8_1:26200368-26200387 | None:intergenic | 45.0% | |
| ! | CCACATTTTTGCAAGAGCAG+TGG | - | chr8_1:26200365-26200384 | Msa1186980:intron | 45.0% |
| GCGTCACATTCTTCAACAGT+TGG | - | chr8_1:26200397-26200416 | Msa1186980:CDS | 45.0% | |
| GAACATGAACCTCATCGAGA+TGG | - | chr8_1:26200421-26200440 | Msa1186980:CDS | 45.0% | |
| CTTTGGTATCCATCTCGATG+AGG | + | chr8_1:26200433-26200452 | None:intergenic | 45.0% | |
| CTCATCGAGATGGATACCAA+AGG | - | chr8_1:26200431-26200450 | Msa1186980:CDS | 45.0% | |
| TGGCTCCCTATGATCCTGAT+TGG | - | chr8_1:26200109-26200128 | Msa1186980:CDS | 50.0% | |
| ! | ATAAGAGGCGGTCTTGGTGT+TGG | - | chr8_1:26200293-26200312 | Msa1186980:intron | 50.0% |
| GGCTCCCACCATAAATCCTT+TGG | + | chr8_1:26200325-26200344 | None:intergenic | 50.0% | |
| ! | GGATTTATGGTGGGAGCCAG+AGG | - | chr8_1:26200327-26200346 | Msa1186980:intron | 55.0% |
| ! | TATGGTGGGAGCCAGAGGAA+TGG | - | chr8_1:26200332-26200351 | Msa1186980:intron | 55.0% |
| GAGGGCGACTTCCATTCCTC+TGG | + | chr8_1:26200346-26200365 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 26200057 | 26200457 | 26200057 | ID=Msa1186980;Name=Msa1186980 |
| chr8_1 | mRNA | 26200057 | 26200457 | 26200057 | ID=Msa1186980-mRNA-1;Parent=Msa1186980;Name=Msa1186980-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|1|0|1|1|1|2|0|92 |
| chr8_1 | exon | 26200367 | 26200457 | 26200367 | ID=Msa1186980-mRNA-1:exon:9290;Parent=Msa1186980-mRNA-1 |
| chr8_1 | exon | 26200057 | 26200244 | 26200057 | ID=Msa1186980-mRNA-1:exon:9289;Parent=Msa1186980-mRNA-1 |
| chr8_1 | CDS | 26200367 | 26200457 | 26200367 | ID=Msa1186980-mRNA-1:cds;Parent=Msa1186980-mRNA-1 |
| chr8_1 | CDS | 26200057 | 26200244 | 26200057 | ID=Msa1186980-mRNA-1:cds;Parent=Msa1186980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
