AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1251170


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGTAATCTGAACGAAAT+TGG 0.193066 8_2:-46962773 None:intergenic
TACCAGAAGATAAGATTATT+TGG 0.207713 8_2:+46962789 Msa1251170:CDS
CTGGTAATCTGAACGAAATT+GGG 0.389759 8_2:-46962772 None:intergenic
TGCCAAATAATCTTATCTTC+TGG 0.431450 8_2:-46962791 None:intergenic
GATTATTTGGCACGGCGAAA+AGG 0.464105 8_2:+46962802 Msa1251170:CDS
GCGCAGGCAGGATCGTCGTC+TGG 0.469415 8_2:+46962881 Msa1251170:CDS
AGGATGCTTTGTACTCGGTC+AGG 0.493366 8_2:+46962822 Msa1251170:CDS
TGAAGAAAAGAGAAGGGCGC+AGG 0.541610 8_2:+46962865 Msa1251170:CDS
GAAAAGAGAAGGGCGCAGGC+AGG 0.547169 8_2:+46962869 Msa1251170:CDS
CGAAAAGGATGCTTTGTACT+CGG 0.565876 8_2:+46962817 Msa1251170:CDS
CAGGTCAGCTTATCATCAAG+TGG 0.582239 8_2:+46962841 Msa1251170:CDS
AAGTGGGTGAAGAAAAGAGA+AGG 0.598929 8_2:+46962858 Msa1251170:CDS
GAAGATAAGATTATTTGGCA+CGG 0.602997 8_2:+46962794 Msa1251170:CDS
AGGTCAGCTTATCATCAAGT+GGG 0.654150 8_2:+46962842 Msa1251170:CDS
AGTGGGTGAAGAAAAGAGAA+GGG 0.676090 8_2:+46962859 Msa1251170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTACATAGAAATCAATT+GGG + chr8_2:46961973-46961992 Msa1251170:intron 15.0%
!! AATTTACATAGAAATCAATT+GGG + chr8_2:46961973-46961992 Msa1251170:intron 15.0%
!!! TTTTTTGTAGTTGTATCTTA+AGG + chr8_2:46961776-46961795 Msa1251170:intron 20.0%
!! GAATTTACATAGAAATCAAT+TGG + chr8_2:46961972-46961991 Msa1251170:intron 20.0%
!! ATTTACATAGAAATCAATTG+GGG + chr8_2:46961974-46961993 Msa1251170:intron 20.0%
!! TTTATCATCCAACATTATAA+AGG - chr8_2:46962043-46962062 None:intergenic 20.0%
!! CAAGTTCAAATTATAAAGAT+AGG + chr8_2:46962113-46962132 Msa1251170:intron 20.0%
!! ATAACAGAAAGTTAAATCAA+TGG - chr8_2:46962150-46962169 None:intergenic 20.0%
!!! TTTTTTGTAGTTGTATCTTA+AGG + chr8_2:46961776-46961795 Msa1251170:intron 20.0%
!! GAATTTACATAGAAATCAAT+TGG + chr8_2:46961972-46961991 Msa1251170:intron 20.0%
!! ATTTACATAGAAATCAATTG+GGG + chr8_2:46961974-46961993 Msa1251170:intron 20.0%
!! TTTATCATCCAACATTATAA+AGG - chr8_2:46962043-46962062 None:intergenic 20.0%
!! CAAGTTCAAATTATAAAGAT+AGG + chr8_2:46962113-46962132 Msa1251170:intron 20.0%
!! ATAACAGAAAGTTAAATCAA+TGG - chr8_2:46962150-46962169 None:intergenic 20.0%
! AACAAAAATGAACGTTACTA+AGG - chr8_2:46961833-46961852 None:intergenic 25.0%
! TACTTTCAACTGAAAAAACA+AGG - chr8_2:46961934-46961953 None:intergenic 25.0%
!! AGTTTGTTCCTTTATAATGT+TGG + chr8_2:46962032-46962051 Msa1251170:intron 25.0%
! CTTTCTGTTATAAATAAGAC+TGG + chr8_2:46962159-46962178 Msa1251170:intron 25.0%
! AATCTATGTTAGCTAAATTC+TGG + chr8_2:46962256-46962275 Msa1251170:intron 25.0%
! TCAAAATCTAGCATGATAAT+TGG + chr8_2:46962592-46962611 Msa1251170:intron 25.0%
! TACCAGAAGATAAGATTATT+TGG + chr8_2:46962789-46962808 Msa1251170:CDS 25.0%
! AACAAAAATGAACGTTACTA+AGG - chr8_2:46961833-46961852 None:intergenic 25.0%
! TACTTTCAACTGAAAAAACA+AGG - chr8_2:46961934-46961953 None:intergenic 25.0%
!! AGTTTGTTCCTTTATAATGT+TGG + chr8_2:46962032-46962051 Msa1251170:intron 25.0%
! CTTTCTGTTATAAATAAGAC+TGG + chr8_2:46962159-46962178 Msa1251170:intron 25.0%
! AATCTATGTTAGCTAAATTC+TGG + chr8_2:46962256-46962275 Msa1251170:intron 25.0%
! TCAAAATCTAGCATGATAAT+TGG + chr8_2:46962592-46962611 Msa1251170:intron 25.0%
! TACCAGAAGATAAGATTATT+TGG + chr8_2:46962789-46962808 Msa1251170:CDS 25.0%
ATGTTGGATGATAAAGAGTA+TGG + chr8_2:46962048-46962067 Msa1251170:intron 30.0%
CTCTCTAATAAAGACAAAGA+AGG + chr8_2:46962216-46962235 Msa1251170:intron 30.0%
TATGTTAGCTAAATTCTGGT+GGG + chr8_2:46962260-46962279 Msa1251170:intron 30.0%
TAATAGAGAACACACTTTGA+TGG + chr8_2:46962422-46962441 Msa1251170:intron 30.0%
AATAGAGAACACACTTTGAT+GGG + chr8_2:46962423-46962442 Msa1251170:intron 30.0%
AAATCAAGATACTATCTGAG+AGG + chr8_2:46962456-46962475 Msa1251170:intron 30.0%
GATATTTACCAAGTTATGCA+TGG + chr8_2:46962502-46962521 Msa1251170:intron 30.0%
!! TTTTGGTCTAAGGTATTCAT+GGG - chr8_2:46962651-46962670 None:intergenic 30.0%
!! ATTTTGGTCTAAGGTATTCA+TGG - chr8_2:46962652-46962671 None:intergenic 30.0%
! ATTGATTCTGATATGAGACA+TGG + chr8_2:46962690-46962709 Msa1251170:intron 30.0%
TGCCAAATAATCTTATCTTC+TGG - chr8_2:46962794-46962813 None:intergenic 30.0%
GAAGATAAGATTATTTGGCA+CGG + chr8_2:46962794-46962813 Msa1251170:CDS 30.0%
ATGTTGGATGATAAAGAGTA+TGG + chr8_2:46962048-46962067 Msa1251170:intron 30.0%
CTCTCTAATAAAGACAAAGA+AGG + chr8_2:46962216-46962235 Msa1251170:intron 30.0%
TATGTTAGCTAAATTCTGGT+GGG + chr8_2:46962260-46962279 Msa1251170:intron 30.0%
TAATAGAGAACACACTTTGA+TGG + chr8_2:46962422-46962441 Msa1251170:intron 30.0%
AATAGAGAACACACTTTGAT+GGG + chr8_2:46962423-46962442 Msa1251170:intron 30.0%
AAATCAAGATACTATCTGAG+AGG + chr8_2:46962456-46962475 Msa1251170:intron 30.0%
GATATTTACCAAGTTATGCA+TGG + chr8_2:46962502-46962521 Msa1251170:intron 30.0%
!! TTTTGGTCTAAGGTATTCAT+GGG - chr8_2:46962651-46962670 None:intergenic 30.0%
!! ATTTTGGTCTAAGGTATTCA+TGG - chr8_2:46962652-46962671 None:intergenic 30.0%
! ATTGATTCTGATATGAGACA+TGG + chr8_2:46962690-46962709 Msa1251170:intron 30.0%
TGCCAAATAATCTTATCTTC+TGG - chr8_2:46962794-46962813 None:intergenic 30.0%
GAAGATAAGATTATTTGGCA+CGG + chr8_2:46962794-46962813 Msa1251170:CDS 30.0%
TACTAAGGTAATTGCAGCAA+AGG - chr8_2:46961818-46961837 None:intergenic 35.0%
CTATGTTAGCTAAATTCTGG+TGG + chr8_2:46962259-46962278 Msa1251170:intron 35.0%
TAAATTCTGGTGGGAATCAA+AGG + chr8_2:46962269-46962288 Msa1251170:intron 35.0%
CTAGCAAAATCCAAAAGTAG+AGG + chr8_2:46962330-46962349 Msa1251170:intron 35.0%
CAAAATCCAAAAGTAGAGGT+GGG + chr8_2:46962334-46962353 Msa1251170:intron 35.0%
TGATTTCAACAAAGCCTCAT+TGG + chr8_2:46962377-46962396 Msa1251170:intron 35.0%
ATAGAGAACACACTTTGATG+GGG + chr8_2:46962424-46962443 Msa1251170:intron 35.0%
ACTTGGTAAATATCCAGTGT+TGG - chr8_2:46962496-46962515 None:intergenic 35.0%
! TTTGGTCTAAGGTATTCATG+GGG - chr8_2:46962650-46962669 None:intergenic 35.0%
!! TACAACAGCATTTTGGTCTA+AGG - chr8_2:46962661-46962680 None:intergenic 35.0%
TTCTGTGTTCAATGAGTTTG+AGG + chr8_2:46962730-46962749 Msa1251170:intron 35.0%
CTGGTAATCTGAACGAAATT+GGG - chr8_2:46962775-46962794 None:intergenic 35.0%
TCTGGTAATCTGAACGAAAT+TGG - chr8_2:46962776-46962795 None:intergenic 35.0%
TACTAAGGTAATTGCAGCAA+AGG - chr8_2:46961818-46961837 None:intergenic 35.0%
CTATGTTAGCTAAATTCTGG+TGG + chr8_2:46962259-46962278 Msa1251170:intron 35.0%
TAAATTCTGGTGGGAATCAA+AGG + chr8_2:46962269-46962288 Msa1251170:intron 35.0%
CTAGCAAAATCCAAAAGTAG+AGG + chr8_2:46962330-46962349 Msa1251170:intron 35.0%
CAAAATCCAAAAGTAGAGGT+GGG + chr8_2:46962334-46962353 Msa1251170:intron 35.0%
TGATTTCAACAAAGCCTCAT+TGG + chr8_2:46962377-46962396 Msa1251170:intron 35.0%
ATAGAGAACACACTTTGATG+GGG + chr8_2:46962424-46962443 Msa1251170:intron 35.0%
ACTTGGTAAATATCCAGTGT+TGG - chr8_2:46962496-46962515 None:intergenic 35.0%
! TTTGGTCTAAGGTATTCATG+GGG - chr8_2:46962650-46962669 None:intergenic 35.0%
!! TACAACAGCATTTTGGTCTA+AGG - chr8_2:46962661-46962680 None:intergenic 35.0%
TTCTGTGTTCAATGAGTTTG+AGG + chr8_2:46962730-46962749 Msa1251170:intron 35.0%
CTGGTAATCTGAACGAAATT+GGG - chr8_2:46962775-46962794 None:intergenic 35.0%
TCTGGTAATCTGAACGAAAT+TGG - chr8_2:46962776-46962795 None:intergenic 35.0%
TAAGGTAATTGCAGCAAAGG+TGG - chr8_2:46961815-46961834 None:intergenic 40.0%
AGACAAAGAAGGATGTTGCT+CGG + chr8_2:46962227-46962246 Msa1251170:intron 40.0%
GCAAAATCCAAAAGTAGAGG+TGG + chr8_2:46962333-46962352 Msa1251170:intron 40.0%
AAAGCCTCATTGGAAAAGCA+TGG + chr8_2:46962387-46962406 Msa1251170:intron 40.0%
CTATCTGAGAGGAAACTTCA+TGG + chr8_2:46962467-46962486 Msa1251170:intron 40.0%
AGCATACTCCATGCATAACT+TGG - chr8_2:46962513-46962532 None:intergenic 40.0%
CTAGCATGATAATTGGCTAC+CGG + chr8_2:46962599-46962618 Msa1251170:intron 40.0%
! GTTCAGCTACAACAGCATTT+TGG - chr8_2:46962668-46962687 None:intergenic 40.0%
CGAAAAGGATGCTTTGTACT+CGG + chr8_2:46962817-46962836 Msa1251170:CDS 40.0%
AGGTCAGCTTATCATCAAGT+GGG + chr8_2:46962842-46962861 Msa1251170:CDS 40.0%
AAGTGGGTGAAGAAAAGAGA+AGG + chr8_2:46962858-46962877 Msa1251170:CDS 40.0%
AGTGGGTGAAGAAAAGAGAA+GGG + chr8_2:46962859-46962878 Msa1251170:CDS 40.0%
TAAGGTAATTGCAGCAAAGG+TGG - chr8_2:46961815-46961834 None:intergenic 40.0%
AGACAAAGAAGGATGTTGCT+CGG + chr8_2:46962227-46962246 Msa1251170:intron 40.0%
GCAAAATCCAAAAGTAGAGG+TGG + chr8_2:46962333-46962352 Msa1251170:intron 40.0%
AAAGCCTCATTGGAAAAGCA+TGG + chr8_2:46962387-46962406 Msa1251170:intron 40.0%
CTATCTGAGAGGAAACTTCA+TGG + chr8_2:46962467-46962486 Msa1251170:intron 40.0%
AGCATACTCCATGCATAACT+TGG - chr8_2:46962513-46962532 None:intergenic 40.0%
CTAGCATGATAATTGGCTAC+CGG + chr8_2:46962599-46962618 Msa1251170:intron 40.0%
! GTTCAGCTACAACAGCATTT+TGG - chr8_2:46962668-46962687 None:intergenic 40.0%
CGAAAAGGATGCTTTGTACT+CGG + chr8_2:46962817-46962836 Msa1251170:CDS 40.0%
AGGTCAGCTTATCATCAAGT+GGG + chr8_2:46962842-46962861 Msa1251170:CDS 40.0%
AAGTGGGTGAAGAAAAGAGA+AGG + chr8_2:46962858-46962877 Msa1251170:CDS 40.0%
AGTGGGTGAAGAAAAGAGAA+GGG + chr8_2:46962859-46962878 Msa1251170:CDS 40.0%
AATTGGGGAACTGAAGTGTG+TGG + chr8_2:46961989-46962008 Msa1251170:intron 45.0%
TTCTGGTGGGAATCAAAGGA+AGG + chr8_2:46962273-46962292 Msa1251170:intron 45.0%
GGGAATCAAAGGAAGGAGAA+AGG + chr8_2:46962280-46962299 Msa1251170:intron 45.0%
ATCCAAAAGTAGAGGTGGGA+TGG + chr8_2:46962338-46962357 Msa1251170:intron 45.0%
TCCAAAAGTAGAGGTGGGAT+GGG + chr8_2:46962339-46962358 Msa1251170:intron 45.0%
! CCAACCATGCTTTTCCAATG+AGG - chr8_2:46962394-46962413 None:intergenic 45.0%
! CCTCATTGGAAAAGCATGGT+TGG + chr8_2:46962391-46962410 Msa1251170:intron 45.0%
AGAACACACTTTGATGGGGA+AGG + chr8_2:46962428-46962447 Msa1251170:intron 45.0%
AACTTCATGGAAGCCAACAC+TGG + chr8_2:46962480-46962499 Msa1251170:intron 45.0%
TGCAGATGGAGGATATGGAA+TGG + chr8_2:46962561-46962580 Msa1251170:intron 45.0%
!! ACTTTGAAGCCAGTTTGTGC+CGG - chr8_2:46962621-46962640 None:intergenic 45.0%
GATTATTTGGCACGGCGAAA+AGG + chr8_2:46962802-46962821 Msa1251170:CDS 45.0%
CAGGTCAGCTTATCATCAAG+TGG + chr8_2:46962841-46962860 Msa1251170:CDS 45.0%
AATTGGGGAACTGAAGTGTG+TGG + chr8_2:46961989-46962008 Msa1251170:intron 45.0%
TTCTGGTGGGAATCAAAGGA+AGG + chr8_2:46962273-46962292 Msa1251170:intron 45.0%
GGGAATCAAAGGAAGGAGAA+AGG + chr8_2:46962280-46962299 Msa1251170:intron 45.0%
ATCCAAAAGTAGAGGTGGGA+TGG + chr8_2:46962338-46962357 Msa1251170:intron 45.0%
TCCAAAAGTAGAGGTGGGAT+GGG + chr8_2:46962339-46962358 Msa1251170:intron 45.0%
! CCAACCATGCTTTTCCAATG+AGG - chr8_2:46962394-46962413 None:intergenic 45.0%
! CCTCATTGGAAAAGCATGGT+TGG + chr8_2:46962391-46962410 Msa1251170:intron 45.0%
AGAACACACTTTGATGGGGA+AGG + chr8_2:46962428-46962447 Msa1251170:intron 45.0%
AACTTCATGGAAGCCAACAC+TGG + chr8_2:46962480-46962499 Msa1251170:intron 45.0%
TGCAGATGGAGGATATGGAA+TGG + chr8_2:46962561-46962580 Msa1251170:intron 45.0%
!! ACTTTGAAGCCAGTTTGTGC+CGG - chr8_2:46962621-46962640 None:intergenic 45.0%
GATTATTTGGCACGGCGAAA+AGG + chr8_2:46962802-46962821 Msa1251170:CDS 45.0%
CAGGTCAGCTTATCATCAAG+TGG + chr8_2:46962841-46962860 Msa1251170:CDS 45.0%
! TCCCATCCCACCTCTACTTT+TGG - chr8_2:46962343-46962362 None:intergenic 50.0%
AGAGGTGGGATGGGATTTAG+AGG + chr8_2:46962348-46962367 Msa1251170:intron 50.0%
TATCCTCCATCTGCACCCTT+TGG - chr8_2:46962556-46962575 None:intergenic 50.0%
! AAGGGTGCAGATGGAGGATA+TGG + chr8_2:46962556-46962575 Msa1251170:intron 50.0%
AATTGGCTACCGGCACAAAC+TGG + chr8_2:46962609-46962628 Msa1251170:intron 50.0%
AGGATGCTTTGTACTCGGTC+AGG + chr8_2:46962822-46962841 Msa1251170:CDS 50.0%
! TGAAGAAAAGAGAAGGGCGC+AGG + chr8_2:46962865-46962884 Msa1251170:CDS 50.0%
! TCCCATCCCACCTCTACTTT+TGG - chr8_2:46962343-46962362 None:intergenic 50.0%
AGAGGTGGGATGGGATTTAG+AGG + chr8_2:46962348-46962367 Msa1251170:intron 50.0%
TATCCTCCATCTGCACCCTT+TGG - chr8_2:46962556-46962575 None:intergenic 50.0%
! AAGGGTGCAGATGGAGGATA+TGG + chr8_2:46962556-46962575 Msa1251170:intron 50.0%
AATTGGCTACCGGCACAAAC+TGG + chr8_2:46962609-46962628 Msa1251170:intron 50.0%
AGGATGCTTTGTACTCGGTC+AGG + chr8_2:46962822-46962841 Msa1251170:CDS 50.0%
! TGAAGAAAAGAGAAGGGCGC+AGG + chr8_2:46962865-46962884 Msa1251170:CDS 50.0%
AGAAGGATGTTGCTCGGAGC+TGG + chr8_2:46962233-46962252 Msa1251170:intron 55.0%
GAGTATGCTGAGTGCGAGAG+TGG + chr8_2:46962524-46962543 Msa1251170:intron 55.0%
CGAGAGTGGTTGTGGCCAAA+GGG + chr8_2:46962538-46962557 Msa1251170:intron 55.0%
! TTGTGGCCAAAGGGTGCAGA+TGG + chr8_2:46962547-46962566 Msa1251170:intron 55.0%
AGAAGGATGTTGCTCGGAGC+TGG + chr8_2:46962233-46962252 Msa1251170:intron 55.0%
GAGTATGCTGAGTGCGAGAG+TGG + chr8_2:46962524-46962543 Msa1251170:intron 55.0%
CGAGAGTGGTTGTGGCCAAA+GGG + chr8_2:46962538-46962557 Msa1251170:intron 55.0%
! TTGTGGCCAAAGGGTGCAGA+TGG + chr8_2:46962547-46962566 Msa1251170:intron 55.0%
GCTGAGTGCGAGAGTGGTTG+TGG + chr8_2:46962530-46962549 Msa1251170:intron 60.0%
GCGAGAGTGGTTGTGGCCAA+AGG + chr8_2:46962537-46962556 Msa1251170:intron 60.0%
! TGGCCAAAGGGTGCAGATGG+AGG + chr8_2:46962550-46962569 Msa1251170:intron 60.0%
! GAAAAGAGAAGGGCGCAGGC+AGG + chr8_2:46962869-46962888 Msa1251170:CDS 60.0%
GCTGAGTGCGAGAGTGGTTG+TGG + chr8_2:46962530-46962549 Msa1251170:intron 60.0%
GCGAGAGTGGTTGTGGCCAA+AGG + chr8_2:46962537-46962556 Msa1251170:intron 60.0%
! TGGCCAAAGGGTGCAGATGG+AGG + chr8_2:46962550-46962569 Msa1251170:intron 60.0%
! GAAAAGAGAAGGGCGCAGGC+AGG + chr8_2:46962869-46962888 Msa1251170:CDS 60.0%
GCGCAGGCAGGATCGTCGTC+TGG + chr8_2:46962881-46962900 Msa1251170:CDS 70.0%
GCGCAGGCAGGATCGTCGTC+TGG + chr8_2:46962881-46962900 Msa1251170:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 46961755 46962922 46961755 ID=Msa1251170;Name=Msa1251170
chr8_2 mRNA 46961755 46962922 46961755 ID=Msa1251170-mRNA-1;Parent=Msa1251170;Name=Msa1251170-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|0.33|0|0.33|3|0|60
chr8_2 exon 46961755 46961760 46961755 ID=Msa1251170-mRNA-1:exon:14213;Parent=Msa1251170-mRNA-1
chr8_2 exon 46961945 46961950 46961945 ID=Msa1251170-mRNA-1:exon:14214;Parent=Msa1251170-mRNA-1
chr8_2 exon 46962752 46962922 46962752 ID=Msa1251170-mRNA-1:exon:14215;Parent=Msa1251170-mRNA-1
chr8_2 CDS 46961755 46961760 46961755 ID=Msa1251170-mRNA-1:cds;Parent=Msa1251170-mRNA-1
chr8_2 CDS 46961945 46961950 46961945 ID=Msa1251170-mRNA-1:cds;Parent=Msa1251170-mRNA-1
chr8_2 CDS 46962752 46962922 46962752 ID=Msa1251170-mRNA-1:cds;Parent=Msa1251170-mRNA-1
Gene Sequence

>Msa1251170

ATGTTGTTGAAAGCAAAACATATTGTTAGCATCCCAATTTCGTTCAGATTACCAGAAGATAAGATTATTTGGCACGGCGAAAAGGATGCTTTGTACTCGGTCAGGTCAGCTTATCATCAAGTGGGTGAAGAAAAGAGAAGGGCGCAGGCAGGATCGTCGTCTGGATCTAATAGAAGCATGTAG

Protein sequence

>Msa1251170

MLLKAKHIVSIPISFRLPEDKIIWHGEKDALYSVRSAYHQVGEEKRRAQAGSSSGSNRSM*