AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0222620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0222620 MtrunA17_Chr4g0072411 100.000 103 0 0 1 103 59 161 1.26e-67 207
Msa0222620 MtrunA17_Chr5g0425951 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr4g0030431 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr3g0100121 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr3g0113681 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr2g0327641 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr3g0103351 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr8g0352791 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr3g0103821 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr3g0104031 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr7g0225731 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr3g0103801 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr3g0103881 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr7g0262791 100.000 103 0 0 1 103 1 103 1.58e-67 204
Msa0222620 MtrunA17_Chr2g0327651 73.333 90 23 1 138 226 22 111 2.60e-39 133
Msa0222620 MtrunA17_Chr3g0115141 44.186 129 66 2 147 270 68 195 3.66e-30 112
Msa0222620 MtrunA17_Chr2g0286171 44.961 129 65 2 147 270 70 197 7.49e-30 111
Msa0222620 MtrunA17_Chr7g0217811 42.857 133 70 2 146 272 64 196 1.92e-28 108
Msa0222620 MtrunA17_Chr4g0003361 43.885 139 72 3 136 270 54 190 3.41e-28 107
Msa0222620 MtrunA17_Chr2g0302541 41.353 133 72 2 146 272 64 196 2.40e-27 105
Msa0222620 MtrunA17_Chr8g0343961 42.963 135 72 2 140 270 61 194 5.13e-27 104
Msa0222620 MtrunA17_Chr8g0343954 40.441 136 76 2 140 271 61 195 5.88e-27 103
Msa0222620 MtrunA17_Chr2g0301611 45.000 120 62 1 155 270 75 194 5.88e-27 103
Msa0222620 MtrunA17_Chr8g0343934 40.741 135 75 2 140 270 61 194 1.27e-26 103
Msa0222620 MtrunA17_Chr4g0012651 41.353 133 72 2 146 272 64 196 1.93e-26 102
Msa0222620 MtrunA17_Chr2g0297231 41.727 139 76 2 136 270 57 194 3.34e-26 102
Msa0222620 MtrunA17_Chr2g0297241 43.165 139 74 2 136 270 57 194 1.01e-25 100
Msa0222620 MtrunA17_Chr2g0302171 42.748 131 69 2 146 270 64 194 1.26e-25 100
Msa0222620 MtrunA17_Chr3g0084511 40.845 142 77 3 136 272 56 195 3.16e-25 99.4
Msa0222620 MtrunA17_Chr2g0313961 43.443 122 65 1 155 272 75 196 3.45e-25 99.4
Msa0222620 MtrunA17_Chr3g0084531 40.845 142 77 3 136 272 57 196 1.11e-24 98.2
Msa0222620 MtrunA17_Chr3g0084521 40.141 142 78 3 136 272 56 195 1.99e-24 97.4
Msa0222620 MtrunA17_Chr8g0344051 37.086 151 90 2 124 270 48 197 2.45e-24 97.4
Msa0222620 MtrunA17_Chr8g0344041 39.062 128 74 1 147 270 71 198 6.14e-24 96.3
Msa0222620 MtrunA17_Chr5g0411961 39.850 133 74 2 146 272 64 196 1.27e-23 95.5
Msa0222620 MtrunA17_Chr8g0344031 36.691 139 83 2 136 270 59 196 4.59e-22 91.3
Msa0222620 MtrunA17_Chr8g0344021 39.370 127 73 1 148 270 71 197 7.95e-22 90.5
Msa0222620 MtrunA17_Chr8g0343911 36.620 142 81 4 136 270 54 193 1.03e-21 90.1
Msa0222620 MtrunA17_Chr8g0343921 34.559 136 84 2 140 270 57 192 7.46e-20 85.1
Msa0222620 MtrunA17_Chr2g0300561 40.000 100 59 1 150 248 70 169 9.41e-19 82.4
Msa0222620 MtrunA17_Chr2g0300591 36.000 100 63 1 150 248 70 169 5.18e-18 80.1
Msa0222620 MtrunA17_Chr2g0300581 36.000 100 63 1 150 248 70 169 7.56e-18 79.7
Msa0222620 MtrunA17_Chr3g0084551 33.835 133 76 3 146 272 64 190 7.66e-16 74.7
Msa0222620 MtrunA17_Chr3g0130241 37.963 108 63 3 156 261 149 254 5.37e-15 73.2
Msa0222620 MtrunA17_Chr1g0210761 39.130 92 55 1 158 248 79 170 8.49e-14 68.6
Msa0222620 MtrunA17_Chr8g0335851 34.043 94 61 1 156 248 86 179 1.12e-13 68.6
Msa0222620 MtrunA17_Chr1g0189841 34.615 104 64 1 149 248 75 178 1.78e-13 68.2
Msa0222620 MtrunA17_Chr1g0193671 33.621 116 76 1 155 269 81 196 2.36e-13 67.8
Msa0222620 MtrunA17_Chr2g0292901 33.981 103 65 1 149 248 72 174 2.38e-13 67.4
Msa0222620 MtrunA17_Chr1g0156131 33.333 105 67 1 149 250 68 172 2.98e-13 67.0
Msa0222620 MtrunA17_Chr6g0449771 35.922 103 63 1 149 248 74 176 3.25e-13 67.4
Msa0222620 MtrunA17_Chr6g0449761 34.711 121 71 2 149 261 74 194 3.26e-13 67.8
Msa0222620 MtrunA17_Chr6g0449751 35.922 103 63 1 149 248 74 176 3.49e-13 67.0
Msa0222620 MtrunA17_Chr6g0449731 35.922 103 63 1 149 248 74 176 3.59e-13 67.0
Msa0222620 MtrunA17_Chr1g0178711 32.653 98 65 1 152 248 87 184 4.51e-13 67.0
Msa0222620 MtrunA17_Chr4g0007041 34.615 104 64 1 149 248 74 177 4.90e-13 66.6
Msa0222620 MtrunA17_Chr2g0292831 33.010 103 66 1 149 248 71 173 6.37e-13 66.2
Msa0222620 MtrunA17_Chr4g0007031 34.615 104 64 1 149 248 74 177 6.40e-13 66.6
Msa0222620 MtrunA17_Chr5g0419971 34.615 104 64 1 149 248 74 177 6.40e-13 66.6
Msa0222620 MtrunA17_Chr5g0419981 34.615 104 64 1 149 248 74 177 6.40e-13 66.6
Msa0222620 MtrunA17_Chr4g0007051 34.615 104 64 1 149 248 74 177 6.72e-13 66.2
Msa0222620 MtrunA17_Chr2g0285871 33.654 104 65 1 149 248 74 177 5.19e-12 63.9
Msa0222620 MtrunA17_Chr2g0321581 34.746 118 75 2 157 272 74 191 5.31e-12 63.5
Msa0222620 MtrunA17_Chr2g0321591 33.898 118 76 2 157 272 74 191 1.57e-11 62.4
Msa0222620 MtrunA17_Chr6g0449791 30.833 120 79 1 133 248 58 177 2.53e-11 61.6
Msa0222620 MtrunA17_Chr1g0193421 35.789 95 60 1 155 248 82 176 4.04e-11 61.2
Msa0222620 MtrunA17_Chr2g0292841 33.654 104 65 2 149 248 36 139 6.83e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0222620 AT3G53730.1 100.000 103 0 0 1 103 1 103 2.18e-67 204
Msa0222620 AT1G07820.2 100.000 103 0 0 1 103 1 103 2.18e-67 204
Msa0222620 AT1G07820.1 100.000 103 0 0 1 103 1 103 2.18e-67 204
Msa0222620 AT5G59690.1 100.000 103 0 0 1 103 1 103 2.18e-67 204
Msa0222620 AT3G46320.1 100.000 103 0 0 1 103 1 103 2.18e-67 204
Msa0222620 AT2G28740.1 100.000 103 0 0 1 103 1 103 2.18e-67 204
Msa0222620 AT1G07660.1 100.000 103 0 0 1 103 1 103 2.18e-67 204
Msa0222620 AT3G45930.1 100.000 103 0 0 1 103 1 103 2.18e-67 204
Msa0222620 AT5G59970.1 100.000 103 0 0 1 103 1 103 2.18e-67 204
Msa0222620 AT5G59970.2 100.000 103 0 0 1 103 1 103 2.18e-67 204
Msa0222620 AT1G07660.2 83.495 103 0 1 1 103 1 86 5.23e-50 160
Msa0222620 AT1G72610.1 35.659 129 78 2 147 270 65 193 2.00e-20 87.0
Msa0222620 AT5G38960.1 40.566 106 59 2 155 257 87 191 3.18e-17 79.0
Msa0222620 AT5G20630.1 32.000 125 80 2 150 269 71 195 5.85e-16 75.1
Msa0222620 AT3G05950.1 36.667 120 69 2 155 267 85 204 1.30e-15 74.3
Msa0222620 AT5G38910.1 40.206 97 55 1 155 248 84 180 1.32e-15 74.3
Msa0222620 AT5G38940.1 41.758 91 50 1 161 248 92 182 2.85e-15 73.6
Msa0222620 AT5G38940.2 41.758 91 50 1 161 248 116 206 4.34e-15 73.2
Msa0222620 AT5G38930.1 41.758 91 50 1 161 248 92 182 7.30e-15 72.4
Msa0222620 AT5G39110.1 39.175 97 55 1 155 247 84 180 1.08e-14 71.6
Msa0222620 AT5G39150.1 39.175 97 55 1 155 247 83 179 1.13e-14 71.6
Msa0222620 AT5G39120.1 39.175 97 55 1 155 247 83 179 1.15e-14 71.6
Msa0222620 AT5G39180.1 39.175 97 55 1 155 247 83 179 1.15e-14 71.6
Msa0222620 AT5G61750.1 35.484 93 59 1 157 248 81 173 3.91e-14 70.1
Msa0222620 AT5G39190.2 37.113 97 57 1 155 247 61 157 1.09e-13 68.6
Msa0222620 AT5G39160.2 37.113 97 57 1 155 247 61 157 1.10e-13 68.6
Msa0222620 AT5G39160.1 37.113 97 57 1 155 247 83 179 1.62e-13 68.6
Msa0222620 AT5G39160.3 37.113 97 57 1 155 247 79 175 1.67e-13 68.6
Msa0222620 AT5G39190.1 37.113 97 57 1 155 247 83 179 1.69e-13 68.6
Msa0222620 AT3G04200.1 35.577 104 63 1 155 254 86 189 3.69e-13 67.8
Msa0222620 AT4G14630.1 38.462 91 52 1 161 247 92 182 5.52e-13 67.0
Msa0222620 AT5G39130.1 35.052 97 59 1 155 247 83 179 8.53e-13 66.6
Msa0222620 AT1G02335.1 37.895 95 58 1 155 248 82 176 1.54e-12 65.9
Msa0222620 AT3G04190.1 33.333 105 66 1 148 248 78 182 2.01e-11 62.8

Find 66 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACAAGGAAGGACTCTTTA+TGG 0.217305 2_1:-71887960 Msa0222620:CDS
TTGCGATGGTATTACTTATT+GGG 0.261353 2_1:-71886963 Msa0222620:CDS
GGAAGGACTCTTTATGGATT+TGG 0.266178 2_1:-71887954 Msa0222620:CDS
CCCGCTGGTTTCCAGTTTGC+TGG 0.285182 2_1:-71885758 Msa0222620:CDS
GTTGCGATGGTATTACTTAT+TGG 0.321655 2_1:-71886964 Msa0222620:CDS
GAAGGACTCTTTATGGATTT+GGG 0.322097 2_1:-71887953 Msa0222620:CDS
AGGAAATGCACGCATCCTTT+AGG 0.367709 2_1:+71885836 None:intergenic
TTGAGTAACAGTGGAGGGTT+TGG 0.367973 2_1:+71885730 None:intergenic
CAACGGCATTGGTATCTCTA+TGG 0.371115 2_1:-71886017 Msa0222620:CDS
GAAGCCTGCGATTCGTCGTT+TGG 0.391757 2_1:-71888144 Msa0222620:CDS
TGGAAAAGGTGGAAAGGGAT+TGG 0.405096 2_1:-71888225 Msa0222620:CDS
TTGTTGCGAATCAAGATGTC+TGG 0.418777 2_1:-71888251 None:intergenic
GGAAAAGGTGGAAAGGGATT+GGG 0.428595 2_1:-71888224 Msa0222620:CDS
GACATTGTTGAATGATTCAA+TGG 0.433344 2_1:+71886078 None:intergenic
GGAAAGGGATTGGGAAAGGG+AGG 0.438545 2_1:-71888215 Msa0222620:CDS
GTTTGGTGTTGCCAGCAAAC+TGG 0.439491 2_1:+71885747 None:intergenic
GAAATTTACTCATCACTGTC+GGG 0.445530 2_1:+71886047 None:intergenic
TTAAAGTAGTGTGGTACTCT+TGG 0.464505 2_1:-71886931 Msa0222620:CDS
TGGTATTACTTATTGGGGTA+TGG 0.468441 2_1:-71886957 Msa0222620:CDS
ATACCAATGCCGTTGAGAGC+TGG 0.477606 2_1:+71886025 None:intergenic
AAGATGTCTGGACGTGGAAA+AGG 0.490201 2_1:-71888239 Msa0222620:CDS
CTCTATGGTTATAGCAGAAG+TGG 0.490592 2_1:-71886002 Msa0222620:CDS
TTTCCAGCTCTCAACGGCAT+TGG 0.491525 2_1:-71886028 Msa0222620:CDS
GGTGGAAAGGGATTGGGAAA+GGG 0.496399 2_1:-71888218 Msa0222620:CDS
GTGCTTCGTGATAACATTCA+AGG 0.499787 2_1:-71888173 Msa0222620:CDS
AGGTGGAAAGGGATTGGGAA+AGG 0.506594 2_1:-71888219 Msa0222620:CDS
GATGTTATTGTGTTTCCTAA+AGG 0.508475 2_1:-71885851 Msa0222620:CDS
GAGAAAGACTGTTACTGCTA+TGG 0.515154 2_1:-71888003 Msa0222620:CDS
GTGCATTTCCTTGTCAACTC+TGG 0.516809 2_1:-71885824 Msa0222620:CDS
TGTTATCACGAAGCACCTTA+CGG 0.542642 2_1:+71888180 None:intergenic
CGAATCAAGATGTCTGGACG+TGG 0.544605 2_1:-71888245 None:intergenic
TGCGATGGTATTACTTATTG+GGG 0.547304 2_1:-71886962 Msa0222620:CDS
TTACTTACACAGAGCACGCT+AGG 0.553235 2_1:-71888025 Msa0222620:CDS
CGTCGTTTGGCACGTAGAGG+AGG 0.553789 2_1:-71888131 Msa0222620:CDS
GTGGCAGCTGAGCTTACAGA+TGG 0.555692 2_1:-71885920 Msa0222620:CDS
TCTCAACTTACAGAAAGAGT+AGG 0.556129 2_1:+71886879 None:intergenic
TTCACCACTGAAAATCCCGC+TGG 0.556809 2_1:-71885773 Msa0222620:CDS
GGAAATTTACTCATCACTGT+CGG 0.559460 2_1:+71886046 None:intergenic
GGACGTGGAAAAGGTGGAAA+GGG 0.559725 2_1:-71888230 Msa0222620:CDS
TATAGCAGAAGTGGGAGCGA+AGG 0.564507 2_1:-71885993 Msa0222620:CDS
AGCTGAGCTTACAGATGGGT+TGG 0.566358 2_1:-71885915 Msa0222620:CDS
TTCTGGCACCAGAGTTGACA+AGG 0.570078 2_1:+71885816 None:intergenic
ATAGCAGAAGTGGGAGCGAA+GGG 0.575293 2_1:-71885992 Msa0222620:CDS
AGGAGCAAAACGACACCGTA+AGG 0.575994 2_1:-71888195 Msa0222620:CDS
CGTGCCAAACGACGAATCGC+AGG 0.582056 2_1:+71888140 None:intergenic
GGAGAATGTTATTCGTGATG+CGG 0.582811 2_1:-71888048 Msa0222620:CDS
ATGTCTGGACGTGGAAAAGG+TGG 0.583297 2_1:-71888236 Msa0222620:CDS
CCAGCAAACTGGAAACCAGC+GGG 0.592210 2_1:+71885758 None:intergenic
TGGACGTGGAAAAGGTGGAA+AGG 0.597841 2_1:-71888231 Msa0222620:CDS
ATGCTTTGAAGAGACAAGGA+AGG 0.603293 2_1:-71887971 Msa0222620:CDS
AAGGACTCTTTATGGATTTG+GGG 0.603730 2_1:-71887952 Msa0222620:CDS
GCACACACATTATGTTGCCA+CGG 0.610699 2_1:-71885957 Msa0222620:CDS
AGTAAATTTCCAGCTCTCAA+CGG 0.614715 2_1:-71886034 Msa0222620:CDS
ATTCGTCGTTTGGCACGTAG+AGG 0.619364 2_1:-71888134 Msa0222620:CDS
GTGGATGCAGGATGTTGCGA+TGG 0.626932 2_1:-71886977 Msa0222620:intron
GTTTATGCTTTGAAGAGACA+AGG 0.627217 2_1:-71887975 Msa0222620:CDS
TCTATGGTTATAGCAGAAGT+GGG 0.629373 2_1:-71886001 Msa0222620:CDS
GGAGGAGTGAAGCGTATAAG+CGG 0.630598 2_1:-71888113 Msa0222620:CDS
ACTCTTGGCTTGTGAAACAC+AGG 0.642689 2_1:-71886916 Msa0222620:CDS
AGGACTCTTTATGGATTTGG+GGG 0.645439 2_1:-71887951 Msa0222620:intron
GTATGGTCATTAAAGTAGTG+TGG 0.664953 2_1:-71886940 Msa0222620:CDS
AAATTTACTCATCACTGTCG+GGG 0.668930 2_1:+71886048 None:intergenic
GCCAGCAAACTGGAAACCAG+CGG 0.684986 2_1:+71885757 None:intergenic
CAACTCTGGTGCCAGAACAG+CGG 0.696690 2_1:-71885810 Msa0222620:CDS
TTGATCTACGAAGAGACACG+TGG 0.703849 2_1:-71888089 Msa0222620:CDS
TGGCAGCTGAGCTTACAGAT+GGG 0.738503 2_1:-71885919 Msa0222620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTATTATAAGTGATTA+TGG - chr2_1:71886054-71886073 Msa0222620:CDS 10.0%
!! TTATTATTATAAGTGATTAT+GGG - chr2_1:71886055-71886074 Msa0222620:CDS 10.0%
!! ATAATTAATGAAAAAGATTA+TGG - chr2_1:71887404-71887423 Msa0222620:intron 10.0%
!! AATTTAATATTGAATAATGC+TGG - chr2_1:71886868-71886887 Msa0222620:intron 15.0%
!!! TGTAAGTTTAAAACTAATTA+TGG + chr2_1:71887366-71887385 None:intergenic 15.0%
!!! TTTTTTTAGGGTAAGAATTT+AGG - chr2_1:71886138-71886157 Msa0222620:intron 20.0%
!!! ATTCAATATCATTTTGTCTA+TGG - chr2_1:71886249-71886268 Msa0222620:intron 20.0%
!! AAATCTTAAAATTCAATTGC+AGG + chr2_1:71886311-71886330 None:intergenic 20.0%
!! AACATTTCAATACTAAAGAT+TGG + chr2_1:71886493-71886512 None:intergenic 20.0%
!! AAAAATTGGAAATATTGCAT+TGG + chr2_1:71886599-71886618 None:intergenic 20.0%
!! TTATATGTAAGTTTAAGGAA+TGG + chr2_1:71887275-71887294 None:intergenic 20.0%
!! ATTATGGATATTTCATTCTA+AGG + chr2_1:71887350-71887369 None:intergenic 20.0%
!! AATTGAATGTACAATAAGAA+AGG + chr2_1:71887587-71887606 None:intergenic 20.0%
!! TTTGAAAAAACTATTAAAGC+TGG - chr2_1:71888064-71888083 Msa0222620:CDS 20.0%
! TATTATAAGTGATTATGGGT+TGG - chr2_1:71886059-71886078 Msa0222620:CDS 25.0%
! ATTATAAGTGATTATGGGTT+GGG - chr2_1:71886060-71886079 Msa0222620:CDS 25.0%
!!! AATTTAGGTGGTGTTTTTTT+AGG - chr2_1:71886125-71886144 Msa0222620:intron 25.0%
!!! ATTTAGGTGGTGTTTTTTTA+GGG - chr2_1:71886126-71886145 Msa0222620:intron 25.0%
!! GAGTAATTATCTTTTGTGAT+AGG - chr2_1:71886160-71886179 Msa0222620:intron 25.0%
!! ATTTTCAAAGTCGTTGTTAA+CGG - chr2_1:71886334-71886353 Msa0222620:intron 25.0%
! ACAATTTGATAGGCAATATT+AGG + chr2_1:71886396-71886415 None:intergenic 25.0%
! AATACTCACAACAATTTGAT+AGG + chr2_1:71886406-71886425 None:intergenic 25.0%
! AAACTAAAACCTAAAACATC+AGG + chr2_1:71886535-71886554 None:intergenic 25.0%
! TGCAGATTTCTTAGAAAAAT+TGG + chr2_1:71886613-71886632 None:intergenic 25.0%
! AGGGTTTATATGTAAGTTTA+AGG + chr2_1:71887280-71887299 None:intergenic 25.0%
! TATAAACCCTAAAGATAGAT+AGG - chr2_1:71887290-71887309 Msa0222620:intron 25.0%
! CATTGTTAAACTCATTTCAA+AGG - chr2_1:71887321-71887340 Msa0222620:intron 25.0%
! TTGTTCAGCTATAAATTTCA+CGG - chr2_1:71887497-71887516 Msa0222620:intron 25.0%
! AAAATTGAATATGCTAACAG+AGG + chr2_1:71887523-71887542 None:intergenic 25.0%
! ATTTAACCATGCAACATTTA+TGG - chr2_1:71887616-71887635 Msa0222620:intron 25.0%
! AAATGAAATCATTGACAATC+AGG + chr2_1:71887813-71887832 None:intergenic 25.0%
!!! GTCAATGATTTCATTTTTTC+TGG - chr2_1:71887817-71887836 Msa0222620:intron 25.0%
ATAGGTAGTTAACTGTTTCA+TGG - chr2_1:71886178-71886197 Msa0222620:intron 30.0%
TTAAAATTCAATTGCAGGCT+TGG + chr2_1:71886306-71886325 None:intergenic 30.0%
TTCCATATATGACGAATTGA+GGG + chr2_1:71886453-71886472 None:intergenic 30.0%
ATTCCATATATGACGAATTG+AGG + chr2_1:71886454-71886473 None:intergenic 30.0%
! ATTGGAAATATTGCATTGGT+TGG + chr2_1:71886595-71886614 None:intergenic 30.0%
CAAATACTATGTGATTGATG+AGG - chr2_1:71886649-71886668 Msa0222620:intron 30.0%
ATTGATGAGGATGTTATTGT+GGG - chr2_1:71886662-71886681 Msa0222620:intron 30.0%
TCACAACTGAAGATGTTTAA+AGG + chr2_1:71886690-71886709 None:intergenic 30.0%
! AGTTATGACCAACTAGATTT+TGG - chr2_1:71886715-71886734 Msa0222620:intron 30.0%
! GTTATGACCAACTAGATTTT+GGG - chr2_1:71886716-71886735 Msa0222620:intron 30.0%
AGATTTCTAAATTCTCCAGA+TGG + chr2_1:71886793-71886812 None:intergenic 30.0%
ATCTGGAGAATTTAGAAATC+TGG - chr2_1:71886792-71886811 Msa0222620:intron 30.0%
TCTGGAGAATTTAGAAATCT+GGG - chr2_1:71886793-71886812 Msa0222620:intron 30.0%
! TTAAGTTGTCTTCTTGAAGT+AGG - chr2_1:71886816-71886835 Msa0222620:intron 30.0%
! TGTTTGGAATTATGTCTTTG+TGG - chr2_1:71886943-71886962 Msa0222620:CDS 30.0%
TTGCGATGGTATTACTTATT+GGG - chr2_1:71886976-71886995 Msa0222620:intron 30.0%
! TTTCTGTAAGTTGAGAAAAG+TGG - chr2_1:71887067-71887086 Msa0222620:intron 30.0%
AAGTTGAGAAAAGTGGAATT+TGG - chr2_1:71887074-71887093 Msa0222620:intron 30.0%
AAGTTTAAGGAATGGATCAT+TGG + chr2_1:71887267-71887286 None:intergenic 30.0%
! ACAAATTTTCTGTGTAAGAG+AGG + chr2_1:71887481-71887500 None:intergenic 30.0%
TGCATATCTTAATCTTCAAG+AGG + chr2_1:71887693-71887712 None:intergenic 30.0%
TTTACATGCATAACCAGAAA+GGG + chr2_1:71887779-71887798 None:intergenic 30.0%
GACATTGTTGAATGATTCAA+TGG + chr2_1:71887864-71887883 None:intergenic 30.0%
GATGTTATTGTGTTTCCTAA+AGG - chr2_1:71888088-71888107 Msa0222620:CDS 30.0%
!! GTTTATGCTTTGAAGAGACA+AGG - chr2_1:71885964-71885983 Msa0222620:CDS 35.0%
GAAGGACTCTTTATGGATTT+GGG - chr2_1:71885986-71886005 Msa0222620:CDS 35.0%
AAGGACTCTTTATGGATTTG+GGG - chr2_1:71885987-71886006 Msa0222620:CDS 35.0%
!! GTCTTGCTGTCAAGAAATTT+TGG - chr2_1:71886087-71886106 Msa0222620:CDS 35.0%
!! AGAAATTTTGGCAAGGTTTG+TGG - chr2_1:71886099-71886118 Msa0222620:intron 35.0%
CAAGGTTTGTGGTTGAATTT+AGG - chr2_1:71886110-71886129 Msa0222620:intron 35.0%
TTGATAGGCAATATTAGGCT+TGG + chr2_1:71886391-71886410 None:intergenic 35.0%
AACCCTCAATTCGTCATATA+TGG - chr2_1:71886448-71886467 Msa0222620:intron 35.0%
GATTGATGAGGATGTTATTG+TGG - chr2_1:71886661-71886680 Msa0222620:intron 35.0%
TCTCCAGATGGAAAAACATA+CGG + chr2_1:71886781-71886800 None:intergenic 35.0%
CTGCAGTAAATTGATCCAAT+GGG + chr2_1:71886902-71886921 None:intergenic 35.0%
ACATAATTCCAAACAGACAG+GGG + chr2_1:71886938-71886957 None:intergenic 35.0%
GACATAATTCCAAACAGACA+GGG + chr2_1:71886939-71886958 None:intergenic 35.0%
AGACATAATTCCAAACAGAC+AGG + chr2_1:71886940-71886959 None:intergenic 35.0%
AATTATGTCTTTGTGGATGC+AGG - chr2_1:71886950-71886969 Msa0222620:CDS 35.0%
GTTGCGATGGTATTACTTAT+TGG - chr2_1:71886975-71886994 Msa0222620:intron 35.0%
TGCGATGGTATTACTTATTG+GGG - chr2_1:71886977-71886996 Msa0222620:intron 35.0%
TGGTATTACTTATTGGGGTA+TGG - chr2_1:71886982-71887001 Msa0222620:intron 35.0%
GTATGGTCATTAAAGTAGTG+TGG - chr2_1:71886999-71887018 Msa0222620:intron 35.0%
TTAAAGTAGTGTGGTACTCT+TGG - chr2_1:71887008-71887027 Msa0222620:intron 35.0%
TCTCAACTTACAGAAAGAGT+AGG + chr2_1:71887063-71887082 None:intergenic 35.0%
! AACGTGTGAGTTTTTCTACT+AGG - chr2_1:71887152-71887171 Msa0222620:intron 35.0%
GAGAGTCCTATCTATCTTTA+GGG + chr2_1:71887299-71887318 None:intergenic 35.0%
AGAGAGTCCTATCTATCTTT+AGG + chr2_1:71887300-71887319 None:intergenic 35.0%
AGAATATGATGAGGATACTG+AGG + chr2_1:71887453-71887472 None:intergenic 35.0%
ACAATAAGAAAGGTTTGCGA+AGG + chr2_1:71887577-71887596 None:intergenic 35.0%
AACCATGCAACATTTATGGA+AGG - chr2_1:71887620-71887639 Msa0222620:intron 35.0%
ATCCTTCCATAAATGTTGCA+TGG + chr2_1:71887625-71887644 None:intergenic 35.0%
TTACATGCATAACCAGAAAG+GGG + chr2_1:71887778-71887797 None:intergenic 35.0%
GTTTACATGCATAACCAGAA+AGG + chr2_1:71887780-71887799 None:intergenic 35.0%
! TTGACAATCAGGTTTTCAGT+TGG + chr2_1:71887802-71887821 None:intergenic 35.0%
AAATTTACTCATCACTGTCG+GGG + chr2_1:71887894-71887913 None:intergenic 35.0%
GAAATTTACTCATCACTGTC+GGG + chr2_1:71887895-71887914 None:intergenic 35.0%
GGAAATTTACTCATCACTGT+CGG + chr2_1:71887896-71887915 None:intergenic 35.0%
AGTAAATTTCCAGCTCTCAA+CGG - chr2_1:71887905-71887924 Msa0222620:intron 35.0%
TCTATGGTTATAGCAGAAGT+GGG - chr2_1:71887938-71887957 Msa0222620:intron 35.0%
! TGTTATCACGAAGCACCTTA+CGG + chr2_1:71885762-71885781 None:intergenic 40.0%
GTGCTTCGTGATAACATTCA+AGG - chr2_1:71885766-71885785 Msa0222620:CDS 40.0%
! TGGCGTGTTGAAGATCTTTT+TGG - chr2_1:71885870-71885889 Msa0222620:CDS 40.0%
GGAGAATGTTATTCGTGATG+CGG - chr2_1:71885891-71885910 Msa0222620:CDS 40.0%
GAGAAAGACTGTTACTGCTA+TGG - chr2_1:71885936-71885955 Msa0222620:CDS 40.0%
!! ATGCTTTGAAGAGACAAGGA+AGG - chr2_1:71885968-71885987 Msa0222620:CDS 40.0%
AGACAAGGAAGGACTCTTTA+TGG - chr2_1:71885979-71885998 Msa0222620:CDS 40.0%
GGAAGGACTCTTTATGGATT+TGG - chr2_1:71885985-71886004 Msa0222620:CDS 40.0%
AGGACTCTTTATGGATTTGG+GGG - chr2_1:71885988-71886007 Msa0222620:CDS 40.0%
TCTTTATGGATTTGGGGGTT+AGG - chr2_1:71885993-71886012 Msa0222620:CDS 40.0%
CTTTATGGATTTGGGGGTTA+GGG - chr2_1:71885994-71886013 Msa0222620:CDS 40.0%
!! GCTGTCAAGAAATTTTGGCA+AGG - chr2_1:71886092-71886111 Msa0222620:CDS 40.0%
GGTTTGTGGTTGAATTTAGG+TGG - chr2_1:71886113-71886132 Msa0222620:intron 40.0%
! CTGTCAACTCCTGATGTTTT+AGG - chr2_1:71886523-71886542 Msa0222620:intron 40.0%
!! AAGAGCACCCAAAATCTAGT+TGG + chr2_1:71886726-71886745 None:intergenic 40.0%
! ATCCCGTATGTTTTTCCATC+TGG - chr2_1:71886775-71886794 Msa0222620:intron 40.0%
CTCCAGATGGAAAAACATAC+GGG + chr2_1:71886780-71886799 None:intergenic 40.0%
AGTTGTCTTCTTGAAGTAGG+TGG - chr2_1:71886819-71886838 Msa0222620:intron 40.0%
ATGCTGGAAAACTATCCCAT+TGG - chr2_1:71886884-71886903 Msa0222620:intron 40.0%
GCTGCAGTAAATTGATCCAA+TGG + chr2_1:71886903-71886922 None:intergenic 40.0%
! ATTTTCTGTGTAAGAGAGGC+AGG + chr2_1:71887477-71887496 None:intergenic 40.0%
! TTTGCGAAGGTTGATCGAAT+AGG + chr2_1:71887564-71887583 None:intergenic 40.0%
CTCTATGGTTATAGCAGAAG+TGG - chr2_1:71887937-71887956 Msa0222620:intron 40.0%
! TGCCACGGAATTTTTGATCA+TGG - chr2_1:71887997-71888016 Msa0222620:CDS 40.0%
CACCATGATCAAAAATTCCG+TGG + chr2_1:71888002-71888021 None:intergenic 40.0%
! CACGGAATTTTTGATCATGG+TGG - chr2_1:71888000-71888019 Msa0222620:CDS 40.0%
!! GTCTTTTGAGTAACAGTGGA+GGG + chr2_1:71888217-71888236 None:intergenic 40.0%
!! TGTCTTTTGAGTAACAGTGG+AGG + chr2_1:71888218-71888237 None:intergenic 40.0%
!! AGCTGTCTTTTGAGTAACAG+TGG + chr2_1:71888221-71888240 None:intergenic 40.0%
TGGAAAAGGTGGAAAGGGAT+TGG - chr2_1:71885714-71885733 Msa0222620:CDS 45.0%
GGAAAAGGTGGAAAGGGATT+GGG - chr2_1:71885715-71885734 Msa0222620:CDS 45.0%
TTGATCTACGAAGAGACACG+TGG - chr2_1:71885850-71885869 Msa0222620:CDS 45.0%
TTACTTACACAGAGCACGCT+AGG - chr2_1:71885914-71885933 Msa0222620:CDS 45.0%
GTCGTTGTTAACGGTTTCTC+TGG - chr2_1:71886343-71886362 Msa0222620:intron 45.0%
!! ACTCTTGGCTTGTGAAACAC+AGG - chr2_1:71887023-71887042 Msa0222620:intron 45.0%
GGAATTTGGAGAAATGCTGC+AGG - chr2_1:71887088-71887107 Msa0222620:intron 45.0%
!! TTCTGGGACTGTAACAGTGT+TGG + chr2_1:71887755-71887774 None:intergenic 45.0%
CATAACCAGAAAGGGGTTCT+GGG + chr2_1:71887771-71887790 None:intergenic 45.0%
!! CAACGGCATTGGTATCTCTA+TGG - chr2_1:71887922-71887941 Msa0222620:intron 45.0%
GCACACACATTATGTTGCCA+CGG - chr2_1:71887982-71888001 Msa0222620:CDS 45.0%
AGGAAATGCACGCATCCTTT+AGG + chr2_1:71888106-71888125 None:intergenic 45.0%
GTGCATTTCCTTGTCAACTC+TGG - chr2_1:71888115-71888134 Msa0222620:CDS 45.0%
TGAAAAAGCAACCGCTGTTC+TGG + chr2_1:71888143-71888162 None:intergenic 45.0%
TTGAGTAACAGTGGAGGGTT+TGG + chr2_1:71888212-71888231 None:intergenic 45.0%
ATGTCTGGACGTGGAAAAGG+TGG - chr2_1:71885703-71885722 Msa0222620:CDS 50.0%
TGGACGTGGAAAAGGTGGAA+AGG - chr2_1:71885708-71885727 Msa0222620:CDS 50.0%
GGACGTGGAAAAGGTGGAAA+GGG - chr2_1:71885709-71885728 Msa0222620:CDS 50.0%
AGGTGGAAAGGGATTGGGAA+AGG - chr2_1:71885720-71885739 Msa0222620:CDS 50.0%
GGTGGAAAGGGATTGGGAAA+GGG - chr2_1:71885721-71885740 Msa0222620:CDS 50.0%
AGGAGCAAAACGACACCGTA+AGG - chr2_1:71885744-71885763 Msa0222620:CDS 50.0%
ATTCGTCGTTTGGCACGTAG+AGG - chr2_1:71885805-71885824 Msa0222620:CDS 50.0%
GGAGGAGTGAAGCGTATAAG+CGG - chr2_1:71885826-71885845 Msa0222620:CDS 50.0%
TCCAGAAACCCCTGTCTGTT+TGG - chr2_1:71886927-71886946 Msa0222620:CDS 50.0%
TCCAAACAGACAGGGGTTTC+TGG + chr2_1:71886931-71886950 None:intergenic 50.0%
GAGGCAGGCAGAATATGATG+AGG + chr2_1:71887462-71887481 None:intergenic 50.0%
GCATAACCAGAAAGGGGTTC+TGG + chr2_1:71887772-71887791 None:intergenic 50.0%
TTTCCAGCTCTCAACGGCAT+TGG - chr2_1:71887911-71887930 Msa0222620:intron 50.0%
ATACCAATGCCGTTGAGAGC+TGG + chr2_1:71887917-71887936 None:intergenic 50.0%
TATAGCAGAAGTGGGAGCGA+AGG - chr2_1:71887946-71887965 Msa0222620:intron 50.0%
ATAGCAGAAGTGGGAGCGAA+GGG - chr2_1:71887947-71887966 Msa0222620:intron 50.0%
TGGCAGCTGAGCTTACAGAT+GGG - chr2_1:71888020-71888039 Msa0222620:CDS 50.0%
AGCTGAGCTTACAGATGGGT+TGG - chr2_1:71888024-71888043 Msa0222620:CDS 50.0%
TTCTGGCACCAGAGTTGACA+AGG + chr2_1:71888126-71888145 None:intergenic 50.0%
TTCACCACTGAAAATCCCGC+TGG - chr2_1:71888166-71888185 Msa0222620:CDS 50.0%
! GAAACCAGCGGGATTTTCAG+TGG + chr2_1:71888173-71888192 None:intergenic 50.0%
!! GTTTGGTGTTGCCAGCAAAC+TGG + chr2_1:71888195-71888214 None:intergenic 50.0%
GGAAAGGGATTGGGAAAGGG+AGG - chr2_1:71885724-71885743 Msa0222620:CDS 55.0%
GAAGCCTGCGATTCGTCGTT+TGG - chr2_1:71885795-71885814 Msa0222620:CDS 55.0%
GTGGATGCAGGATGTTGCGA+TGG - chr2_1:71886962-71886981 Msa0222620:CDS 55.0%
ACAGTCCCAGAACCCCTTTC+TGG - chr2_1:71887763-71887782 Msa0222620:intron 55.0%
GTGGCAGCTGAGCTTACAGA+TGG - chr2_1:71888019-71888038 Msa0222620:CDS 55.0%
! CAACTCTGGTGCCAGAACAG+CGG - chr2_1:71888129-71888148 Msa0222620:CDS 55.0%
CCAGCAAACTGGAAACCAGC+GGG + chr2_1:71888184-71888203 None:intergenic 55.0%
GCCAGCAAACTGGAAACCAG+CGG + chr2_1:71888185-71888204 None:intergenic 55.0%
CGTGCCAAACGACGAATCGC+AGG + chr2_1:71885802-71885821 None:intergenic 60.0%
CGTCGTTTGGCACGTAGAGG+AGG - chr2_1:71885808-71885827 Msa0222620:CDS 60.0%
!! CCCGCTGGTTTCCAGTTTGC+TGG - chr2_1:71888181-71888200 Msa0222620:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 71885703 71888258 71885703 ID=Msa0222620;Name=Msa0222620
chr2_1 mRNA 71885703 71888258 71885703 ID=Msa0222620-mRNA-1;Parent=Msa0222620;Name=Msa0222620-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|0|0|0.66|1|1|3|0|272
chr2_1 exon 71887952 71888258 71887952 ID=Msa0222620-mRNA-1:exon:20734;Parent=Msa0222620-mRNA-1
chr2_1 exon 71886890 71886989 71886890 ID=Msa0222620-mRNA-1:exon:20733;Parent=Msa0222620-mRNA-1
chr2_1 exon 71885703 71886114 71885703 ID=Msa0222620-mRNA-1:exon:20732;Parent=Msa0222620-mRNA-1
chr2_1 CDS 71887952 71888258 71887952 ID=Msa0222620-mRNA-1:cds;Parent=Msa0222620-mRNA-1
chr2_1 CDS 71886890 71886989 71886890 ID=Msa0222620-mRNA-1:cds;Parent=Msa0222620-mRNA-1
chr2_1 CDS 71885703 71886114 71885703 ID=Msa0222620-mRNA-1:cds;Parent=Msa0222620-mRNA-1
Gene Sequence

>Msa0222620

ATGTCTGGACGTGGAAAAGGTGGAAAGGGATTGGGAAAGGGAGGAGCAAAACGACACCGTAAGGTGCTTCGTGATAACATTCAAGGAATAACGAAGCCTGCGATTCGTCGTTTGGCACGTAGAGGAGGAGTGAAGCGTATAAGCGGTTTGATCTACGAAGAGACACGTGGCGTGTTGAAGATCTTTTTGGAGAATGTTATTCGTGATGCGGTTACTTACACAGAGCACGCTAGGAGAAAGACTGTTACTGCTATGGATGTTGTTTATGCTTTGAAGAGACAAGGAAGGACTCTTTATGGATTTGGGGGATGTTGCGATGGTATTACTTATTGGGGTATGGTCATTAAAGTAGTGTGGTACTCTTGGCTTGTGAAACACAGGAAATTTGTGTTGATCCTACTCTTTCTTCTAAGAAAGCAAACCATTGAATCATTCAACAATGTCAAGCTTACCCCGACAGTGATGAGTAAATTTCCAGCTCTCAACGGCATTGGTATCTCTATGGTTATAGCAGAAGTGGGAGCGAAGGGAAGCTACTCTATGCACACACATTATGTTGCCACGGAATTTTTGATCATGGTGGCAGCTGAGCTTACAGATGGGTTGGTGATAGATGAAGAAGTGTTTGAAAAAACTATTAAAGCTGGAGATGTTATTGTGTTTCCTAAAGGATGCGTGCATTTCCTTGTCAACTCTGGTGCCAGAACAGCGGTTGCTTTTTCAGCTTTCACCACTGAAAATCCCGCTGGTTTCCAGTTTGCTGGCAACACCAAACCCTCCACTGTTACTCAAAAGACAGCTCTTCTTGATTTTGAATAA
Protein sequence

>Msa0222620

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGGCCDGITYWGMVIKVVWYSWLVKHRKFVLILLFLLRKQTIESFNNVKLTPTVMSKFPALNGIGISMVIAEVGAKGSYSMHTHYVATEFLIMVAAELTDGLVIDEEVFEKTIKAGDVIVFPKGCVHFLVNSGARTAVAFSAFTTENPAGFQFAGNTKPSTVTQKTALLDFE*