AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1361400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1361400 MtrunA17_Chr4g0072411 100.000 103 0 0 1 103 59 161 5.39e-69 203
Msa1361400 MtrunA17_Chr5g0425951 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr4g0030431 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr3g0100121 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr3g0113681 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr2g0327641 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr3g0103351 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr8g0352791 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr3g0103821 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr3g0104031 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr7g0225731 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr3g0103801 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr3g0103881 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1361400 MtrunA17_Chr7g0262791 100.000 103 0 0 1 103 1 103 2.26e-68 199
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1361400 AT3G53730.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1361400 AT1G07820.2 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1361400 AT1G07820.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1361400 AT5G59690.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1361400 AT3G46320.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1361400 AT2G28740.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1361400 AT1G07660.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1361400 AT3G45930.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1361400 AT5G59970.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1361400 AT5G59970.2 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1361400 AT1G07660.2 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 30 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCTCCGCGACAACATTC+AGG 0.329692 tig0017492:-52723 Msa1361400:CDS
GGAAGGACTCTTTACGGTTT+CGG 0.343515 tig0017492:-52503 Msa1361400:CDS
GGCAAAGGAGGAAAGGGTTT+AGG 0.384869 tig0017492:-52773 Msa1361400:CDS
AGGCAGGGAAGGACTCTTTA+CGG 0.424564 tig0017492:-52509 Msa1361400:CDS
GGAGGAAAGGGTTTAGGAAA+GGG 0.429069 tig0017492:-52767 Msa1361400:CDS
TCAACTTTCGCAGCTATGTC+AGG 0.463992 tig0017492:-52800 None:intergenic
TGTTTACGCTCTCAAGAGGC+AGG 0.493747 tig0017492:-52525 Msa1361400:CDS
TGTTGTCGCGGAGAACCTTA+CGG 0.502023 tig0017492:+52729 None:intergenic
AGGAAGAGGCAAAGGAGGAA+AGG 0.518412 tig0017492:-52780 Msa1361400:CDS
GGAAGAGGCAAAGGAGGAAA+GGG 0.527098 tig0017492:-52779 Msa1361400:CDS
AGGAGGAAAGGGTTTAGGAA+AGG 0.528235 tig0017492:-52768 Msa1361400:CDS
GGAAAGGGTTTAGGAAAGGG+AGG 0.536831 tig0017492:-52764 Msa1361400:CDS
CCATCGCAGTAACAGTCTTA+CGG 0.545551 tig0017492:+52552 None:intergenic
GTTCTCCGCGACAACATTCA+GGG 0.554706 tig0017492:-52722 Msa1361400:CDS
CCGTAAGACTGTTACTGCGA+TGG 0.561095 tig0017492:-52552 Msa1361400:CDS
TTCGCAGCTATGTCAGGAAG+AGG 0.562303 tig0017492:-52794 None:intergenic
GCTATGTCAGGAAGAGGCAA+AGG 0.571124 tig0017492:-52788 Msa1361400:CDS
AGGAGCAAAACGACACCGTA+AGG 0.575994 tig0017492:-52744 Msa1361400:CDS
ACGCTCTCAAGAGGCAGGGA+AGG 0.583359 tig0017492:-52520 Msa1361400:CDS
CTTGCAAGACGACGAATCGC+AGG 0.588436 tig0017492:+52689 None:intergenic
ACGGCGAGCATGCTCTGTGT+AGG 0.590446 tig0017492:+52571 None:intergenic
ATGTCAGGAAGAGGCAAAGG+AGG 0.592343 tig0017492:-52785 Msa1361400:CDS
ATGTTGTTTACGCTCTCAAG+AGG 0.616421 tig0017492:-52529 Msa1361400:CDS
GTTTACGCTCTCAAGAGGCA+GGG 0.624823 tig0017492:-52524 Msa1361400:CDS
CGTCGTCTTGCAAGACGTGG+TGG 0.633046 tig0017492:-52680 Msa1361400:CDS
TTAATCTATGAAGAAACTCG+TGG 0.634992 tig0017492:-52638 Msa1361400:CDS
TAATTCCCTGAATGTTGTCG+CGG 0.640736 tig0017492:+52717 None:intergenic
ATTCGTCGTCTTGCAAGACG+TGG 0.646636 tig0017492:-52683 Msa1361400:CDS
AGGACTCTTTACGGTTTCGG+CGG 0.659680 tig0017492:-52500 Msa1361400:CDS
GGTGGTGTGAAACGTATCAG+TGG 0.722771 tig0017492:-52662 Msa1361400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTAATCTATGAAGAAACTCG+TGG - tig0017492:52643-52662 Msa1361400:CDS 30.0%
TAATTCCCTGAATGTTGTCG+CGG + tig0017492:52567-52586 None:intergenic 40.0%
! ATGTTGTTTACGCTCTCAAG+AGG - tig0017492:52752-52771 Msa1361400:CDS 40.0%
AGGAGGAAAGGGTTTAGGAA+AGG - tig0017492:52513-52532 Msa1361400:CDS 45.0%
GGAGGAAAGGGTTTAGGAAA+GGG - tig0017492:52514-52533 Msa1361400:CDS 45.0%
CCATCGCAGTAACAGTCTTA+CGG + tig0017492:52732-52751 None:intergenic 45.0%
GGAAGGACTCTTTACGGTTT+CGG - tig0017492:52778-52797 Msa1361400:CDS 45.0%
ATGTCAGGAAGAGGCAAAGG+AGG - tig0017492:52496-52515 Msa1361400:CDS 50.0%
AGGAAGAGGCAAAGGAGGAA+AGG - tig0017492:52501-52520 Msa1361400:CDS 50.0%
GGAAGAGGCAAAGGAGGAAA+GGG - tig0017492:52502-52521 Msa1361400:CDS 50.0%
GGCAAAGGAGGAAAGGGTTT+AGG - tig0017492:52508-52527 Msa1361400:CDS 50.0%
GGAAAGGGTTTAGGAAAGGG+AGG - tig0017492:52517-52536 Msa1361400:CDS 50.0%
AGGAGCAAAACGACACCGTA+AGG - tig0017492:52537-52556 Msa1361400:CDS 50.0%
TGTTGTCGCGGAGAACCTTA+CGG + tig0017492:52555-52574 None:intergenic 50.0%
GTTCTCCGCGACAACATTCA+GGG - tig0017492:52559-52578 Msa1361400:CDS 50.0%
ATTCGTCGTCTTGCAAGACG+TGG - tig0017492:52598-52617 Msa1361400:CDS 50.0%
GGTGGTGTGAAACGTATCAG+TGG - tig0017492:52619-52638 Msa1361400:CDS 50.0%
CCGTAAGACTGTTACTGCGA+TGG - tig0017492:52729-52748 Msa1361400:CDS 50.0%
TGTTTACGCTCTCAAGAGGC+AGG - tig0017492:52756-52775 Msa1361400:CDS 50.0%
GTTTACGCTCTCAAGAGGCA+GGG - tig0017492:52757-52776 Msa1361400:CDS 50.0%
AGGCAGGGAAGGACTCTTTA+CGG - tig0017492:52772-52791 Msa1361400:CDS 50.0%
AGGACTCTTTACGGTTTCGG+CGG - tig0017492:52781-52800 Msa1361400:CDS 50.0%
GGTTCTCCGCGACAACATTC+AGG - tig0017492:52558-52577 Msa1361400:CDS 55.0%
CTTGCAAGACGACGAATCGC+AGG + tig0017492:52595-52614 None:intergenic 55.0%
CGTCGTCTTGCAAGACGTGG+TGG - tig0017492:52601-52620 Msa1361400:CDS 60.0%
ACGGCGAGCATGCTCTGTGT+AGG + tig0017492:52713-52732 None:intergenic 60.0%
ACGCTCTCAAGAGGCAGGGA+AGG - tig0017492:52761-52780 Msa1361400:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0017492 gene 52496 52807 52496 ID=Msa1361400;Name=Msa1361400
tig0017492 mRNA 52496 52807 52496 ID=Msa1361400-mRNA-1;Parent=Msa1361400;Name=Msa1361400-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|103
tig0017492 exon 52496 52807 52496 ID=Msa1361400-mRNA-1:exon:12387;Parent=Msa1361400-mRNA-1
tig0017492 CDS 52496 52807 52496 ID=Msa1361400-mRNA-1:cds;Parent=Msa1361400-mRNA-1
Gene Sequence

>Msa1361400

ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTCCGCGACAACATTCAGGGAATTACAAAGCCTGCGATTCGTCGTCTTGCAAGACGTGGTGGTGTGAAACGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAGACTGTTACTGCGATGGATGTTGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTACGGTTTCGGCGGTTAG

Protein sequence

>Msa1361400

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*