Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435650 | AES73656.1 | 68.085 | 47 | 14 | 1 | 7 | 53 | 32 | 77 | 2.70e-11 | 67.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435650 | G7JB28 | 68.085 | 47 | 14 | 1 | 7 | 53 | 32 | 77 | 1.29e-11 | 67.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0073530 | Msa1435650 | 0.830707 | 2.523440e-55 | -8.615850e-47 |
| Msa1357530 | Msa1435650 | 0.816530 | 5.272824e-52 | -8.615850e-47 |
| Msa1359040 | Msa1435650 | 0.811770 | 5.929705e-51 | -8.615850e-47 |
| Msa1372760 | Msa1435650 | 0.804946 | 1.692506e-49 | -8.615850e-47 |
| Msa1376890 | Msa1435650 | 0.804057 | 2.592451e-49 | -8.615850e-47 |
| Msa1378760 | Msa1435650 | 0.801471 | 8.865925e-49 | -8.615850e-47 |
| Msa1384350 | Msa1435650 | 0.803595 | 3.233569e-49 | -8.615850e-47 |
| Msa1392260 | Msa1435650 | 0.812888 | 3.380350e-51 | -8.615850e-47 |
| Msa1393410 | Msa1435650 | 0.802128 | 6.499638e-49 | -8.615850e-47 |
| Msa1411020 | Msa1435650 | 0.811647 | 6.307002e-51 | -8.615850e-47 |
| Msa1420290 | Msa1435650 | 0.818525 | 1.873050e-52 | -8.615850e-47 |
| Msa1424290 | Msa1435650 | 0.807914 | 4.004819e-50 | -8.615850e-47 |
| Msa1429900 | Msa1435650 | 0.809084 | 2.253551e-50 | -8.615850e-47 |
| Msa1434350 | Msa1435650 | 0.807952 | 3.930427e-50 | -8.615850e-47 |
| Msa1435420 | Msa1435650 | 0.801878 | 7.314647e-49 | -8.615850e-47 |
| Msa1435650 | Msa1439320 | 0.809823 | 1.563920e-50 | -8.615850e-47 |
| Msa0657970 | Msa1435650 | 0.821344 | 4.243462e-53 | -8.615850e-47 |
| Msa0658570 | Msa1435650 | 0.809664 | 1.691742e-50 | -8.615850e-47 |
| Msa0661870 | Msa1435650 | 0.804556 | 2.041153e-49 | -8.615850e-47 |
| Msa0693380 | Msa1435650 | 0.828419 | 9.084489e-55 | -8.615850e-47 |
| Msa0703490 | Msa1435650 | 0.803146 | 4.007189e-49 | -8.615850e-47 |
| Msa0759410 | Msa1435650 | 0.804319 | 2.287270e-49 | -8.615850e-47 |
| Msa0760130 | Msa1435650 | 0.803726 | 3.038154e-49 | -8.615850e-47 |
| Msa0766630 | Msa1435650 | 0.815528 | 8.828187e-52 | -8.615850e-47 |
| Msa0188830 | Msa1435650 | 0.811958 | 5.394547e-51 | -8.615850e-47 |
| Msa0223410 | Msa1435650 | -0.803945 | 2.735321e-49 | -8.615850e-47 |
| Msa1233890 | Msa1435650 | 0.802037 | 6.784610e-49 | -8.615850e-47 |
| Msa1238470 | Msa1435650 | 0.827709 | 1.346841e-54 | -8.615850e-47 |
| Msa1249490 | Msa1435650 | 0.812868 | 3.413072e-51 | -8.615850e-47 |
| Msa1283860 | Msa1435650 | 0.816385 | 5.681853e-52 | -8.615850e-47 |
| Msa1291660 | Msa1435650 | 0.800470 | 1.419495e-48 | -8.615850e-47 |
| Msa1308620 | Msa1435650 | 0.800966 | 1.124583e-48 | -8.615850e-47 |
| Msa1322890 | Msa1435650 | 0.806105 | 9.668734e-50 | -8.615850e-47 |
| Msa1324780 | Msa1435650 | 0.813736 | 2.200712e-51 | -8.615850e-47 |
| Msa1329700 | Msa1435650 | 0.800417 | 1.455784e-48 | -8.615850e-47 |
| Msa1342840 | Msa1435650 | 0.804147 | 2.483957e-49 | -8.615850e-47 |
| Msa0373930 | Msa1435650 | 0.805258 | 1.455935e-49 | -8.615850e-47 |
| Msa0417100 | Msa1435650 | 0.815972 | 7.027133e-52 | -8.615850e-47 |
| Msa0432290 | Msa1435650 | 0.813980 | 1.944856e-51 | -8.615850e-47 |
| Msa0432300 | Msa1435650 | 0.945648 | 2.058181e-104 | -8.615850e-47 |
| Msa0437990 | Msa1435650 | 0.816537 | 5.255074e-52 | -8.615850e-47 |
| Msa1014540 | Msa1435650 | 0.811511 | 6.750268e-51 | -8.615850e-47 |
| Msa1059570 | Msa1435650 | 0.800997 | 1.108544e-48 | -8.615850e-47 |
| Msa1075220 | Msa1435650 | -0.811897 | 5.562835e-51 | -8.615850e-47 |
| Msa1079180 | Msa1435650 | 0.810242 | 1.270816e-50 | -8.615850e-47 |
| Msa1082500 | Msa1435650 | 0.837605 | 4.709921e-57 | -8.615850e-47 |
| Msa0331920 | Msa1435650 | 0.813498 | 2.482492e-51 | -8.615850e-47 |
| Msa1107800 | Msa1435650 | 0.803278 | 3.761703e-49 | -8.615850e-47 |
| Msa1116130 | Msa1435650 | -0.801953 | 7.059976e-49 | -8.615850e-47 |
| Msa1117170 | Msa1435650 | 0.803119 | 4.059337e-49 | -8.615850e-47 |
| Msa1139000 | Msa1435650 | -0.804751 | 1.858239e-49 | -8.615850e-47 |
| Msa1162550 | Msa1435650 | -0.809526 | 1.811706e-50 | -8.615850e-47 |
| Msa1195920 | Msa1435650 | 0.824482 | 7.880603e-54 | -8.615850e-47 |
| Msa0484780 | Msa1435650 | 0.812846 | 3.451939e-51 | -8.615850e-47 |
| Msa0512900 | Msa1435650 | 0.818716 | 1.695121e-52 | -8.615850e-47 |
| Msa0532350 | Msa1435650 | 0.810763 | 9.803866e-51 | -8.615850e-47 |
| Msa0533070 | Msa1435650 | 0.801315 | 9.541780e-49 | -8.615850e-47 |
| Msa0558690 | Msa1435650 | 0.808462 | 3.060098e-50 | -8.615850e-47 |
| Msa0559190 | Msa1435650 | 0.807459 | 5.002737e-50 | -8.615850e-47 |
| Msa0567020 | Msa1435650 | 0.805909 | 1.063152e-49 | -8.615850e-47 |
| Msa0885280 | Msa1435650 | 0.828011 | 1.139181e-54 | -8.615850e-47 |
| Msa0909120 | Msa1435650 | 0.838961 | 2.106167e-57 | -8.615850e-47 |
| Msa0918220 | Msa1435650 | 0.808555 | 2.924642e-50 | -8.615850e-47 |
| Msa0954130 | Msa1435650 | 0.801598 | 8.347589e-49 | -8.615850e-47 |
| Msa0954550 | Msa1435650 | 0.803265 | 3.786416e-49 | -8.615850e-47 |
| Msa0959770 | Msa1435650 | 0.805608 | 1.229832e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCGATTGACACAATTAT+TGG | 0.171926 | tig0027765:+20421 | Msa1435640:intron |
| TTCTGGCGGAGTTTCAATAT+TGG | 0.300547 | tig0027765:+20721 | Msa1435640:intron |
| ACAACAGAAGCTCCATATTC+AGG | 0.357092 | tig0027765:+20394 | Msa1435640:intron |
| CACCAAGGTTGTGGAGCGTT+TGG | 0.362879 | tig0027765:-20833 | Msa1435650:CDS |
| ATCGAAGCCAAGCCTGAATA+TGG | 0.364772 | tig0027765:-20406 | Msa1435650:CDS |
| GGTAGTTGTTCAATATCTTC+TGG | 0.404192 | tig0027765:+20704 | Msa1435640:intron |
| GCATAGAGAAGGTGCGTTGA+AGG | 0.466723 | tig0027765:-20902 | Msa1435650:CDS |
| TGTAAATCCGACTGGGATGC+AGG | 0.468237 | tig0027765:-20875 | Msa1435650:CDS |
| TTATAGAATCACCAAGGTTG+TGG | 0.498080 | tig0027765:-20842 | Msa1435650:CDS |
| AAGGGAAATGTAAATCCGAC+TGG | 0.506040 | tig0027765:-20883 | Msa1435650:CDS |
| AGAAGCTCCATATTCAGGCT+TGG | 0.518362 | tig0027765:+20399 | Msa1435640:intron |
| CATAGAGAAGGTGCGTTGAA+GGG | 0.537585 | tig0027765:-20901 | Msa1435650:CDS |
| CTATGGCTCTCCGTCTCCTG+AGG | 0.555426 | tig0027765:-20761 | Msa1435650:CDS |
| AGCCAAACGCTCCACAACCT+TGG | 0.564815 | tig0027765:+20831 | Msa1435640:intron |
| AGGTTGTGGAGCGTTTGGCT+AGG | 0.588926 | tig0027765:-20828 | Msa1435650:CDS |
| TATGGCTCTCCGTCTCCTGA+GGG | 0.597298 | tig0027765:-20760 | Msa1435650:CDS |
| ATAATCAATCCCTCAGGAGA+CGG | 0.598701 | tig0027765:+20751 | Msa1435640:intron |
| AAGTGCATAATCAATCCCTC+AGG | 0.601496 | tig0027765:+20745 | Msa1435640:intron |
| TTTATGGCACTGCATAGAGA+AGG | 0.645189 | tig0027765:-20913 | Msa1435650:CDS |
| TGCATATTCTTCGACAACAT+AGG | 0.650468 | tig0027765:+20683 | Msa1435640:intron |
| TGATGAATTAGCAAGTGTGA+TGG | 0.651180 | tig0027765:-20647 | Msa1435650:intron |
| TTGATGACCTGCATCCCAGT+CGG | 0.656366 | tig0027765:+20868 | Msa1435640:intron |
| AGTTGTTCAATATCTTCTGG+CGG | 0.661594 | tig0027765:+20707 | Msa1435640:intron |
| AGGGAAATGTAAATCCGACT+GGG | 0.662637 | tig0027765:-20882 | Msa1435650:CDS |
| CAACTATTATAGAATCACCA+AGG | 0.712891 | tig0027765:-20848 | Msa1435650:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTCAAATTTTTTAAGCTA+TGG | - | tig0027765:20504-20523 | Msa1435650:intron | 15.0% |
| !!! | GAAAATTCGATTTTATACTT+GGG | + | tig0027765:20489-20508 | Msa1435640:intron | 20.0% |
| !!! | TGAAAATTCGATTTTATACT+TGG | + | tig0027765:20490-20509 | Msa1435640:intron | 20.0% |
| !! | TTTCTATCCAAATATGAATT+AGG | - | tig0027765:20836-20855 | Msa1435650:CDS | 20.0% |
| !! | TTCTATCCAAATATGAATTA+GGG | - | tig0027765:20837-20856 | Msa1435650:CDS | 20.0% |
| ! | AAATTTACATGCATTCTTGA+CGG | + | tig0027765:20673-20692 | Msa1435640:intron | 25.0% |
| !!! | CTTTTGACTCTAATTTGTTA+AGG | + | tig0027765:20704-20723 | Msa1435640:intron | 25.0% |
| CAACTATTATAGAATCACCA+AGG | - | tig0027765:20434-20453 | Msa1435650:intron | 30.0% | |
| GTCAAGAATGCATGTAAATT+TGG | - | tig0027765:20672-20691 | Msa1435650:CDS | 30.0% | |
| AGAATGCATGTAAATTTGGA+TGG | - | tig0027765:20676-20695 | Msa1435650:CDS | 30.0% | |
| TTAACTATTACTCGTGAGAT+AGG | - | tig0027765:20729-20748 | Msa1435650:CDS | 30.0% | |
| ATTGCAGAATAACAAGTACT+AGG | + | tig0027765:20795-20814 | Msa1435640:intron | 30.0% | |
| !! | TAGTACTTGTTATTCTGCAA+TGG | - | tig0027765:20794-20813 | Msa1435650:CDS | 30.0% |
| ATTGGACCCTAATTCATATT+TGG | + | tig0027765:20846-20865 | Msa1435640:intron | 30.0% | |
| TTATAGAATCACCAAGGTTG+TGG | - | tig0027765:20440-20459 | Msa1435650:intron | 35.0% | |
| !!! | CGATTTTATACTTGGGTTGA+AGG | + | tig0027765:20482-20501 | Msa1435640:intron | 35.0% |
| AGTTGTTCAATATCTTCTGG+CGG | + | tig0027765:20578-20597 | Msa1435640:intron | 35.0% | |
| GGTAGTTGTTCAATATCTTC+TGG | + | tig0027765:20581-20600 | Msa1435640:intron | 35.0% | |
| TGCATATTCTTCGACAACAT+AGG | + | tig0027765:20602-20621 | Msa1435640:intron | 35.0% | |
| TGATGAATTAGCAAGTGTGA+TGG | - | tig0027765:20635-20654 | Msa1435650:intron | 35.0% | |
| GTTACTTATCACGTTTCTGA+GGG | + | tig0027765:20769-20788 | Msa1435640:intron | 35.0% | |
| ! | TGTTACTTATCACGTTTCTG+AGG | + | tig0027765:20770-20789 | Msa1435640:intron | 35.0% |
| GCTTCGATTGACACAATTAT+TGG | + | tig0027765:20864-20883 | Msa1435640:intron | 35.0% | |
| AAGGGAAATGTAAATCCGAC+TGG | - | tig0027765:20399-20418 | Msa1435650:CDS | 40.0% | |
| AGGGAAATGTAAATCCGACT+GGG | - | tig0027765:20400-20419 | Msa1435650:CDS | 40.0% | |
| ATAATCAATCCCTCAGGAGA+CGG | + | tig0027765:20534-20553 | Msa1435640:intron | 40.0% | |
| AAGTGCATAATCAATCCCTC+AGG | + | tig0027765:20540-20559 | Msa1435640:intron | 40.0% | |
| TTCTGGCGGAGTTTCAATAT+TGG | + | tig0027765:20564-20583 | Msa1435640:intron | 40.0% | |
| ACAACAGAAGCTCCATATTC+AGG | + | tig0027765:20891-20910 | Msa1435640:intron | 40.0% | |
| ! | CATAGAGAAGGTGCGTTGAA+GGG | - | tig0027765:20381-20400 | Msa1435650:CDS | 45.0% |
| ATCGAAGCCAAGCCTGAATA+TGG | - | tig0027765:20876-20895 | Msa1435650:CDS | 45.0% | |
| AGAAGCTCCATATTCAGGCT+TGG | + | tig0027765:20886-20905 | Msa1435640:intron | 45.0% | |
| ! | GCATAGAGAAGGTGCGTTGA+AGG | - | tig0027765:20380-20399 | Msa1435650:CDS | 50.0% |
| TGTAAATCCGACTGGGATGC+AGG | - | tig0027765:20407-20426 | Msa1435650:CDS | 50.0% | |
| ! | TTGATGACCTGCATCCCAGT+CGG | + | tig0027765:20417-20436 | Msa1435640:intron | 50.0% |
| ! | CACCAAGGTTGTGGAGCGTT+TGG | - | tig0027765:20449-20468 | Msa1435650:intron | 55.0% |
| AGCCAAACGCTCCACAACCT+TGG | + | tig0027765:20454-20473 | Msa1435640:intron | 55.0% | |
| ! | AGGTTGTGGAGCGTTTGGCT+AGG | - | tig0027765:20454-20473 | Msa1435650:intron | 55.0% |
| TATGGCTCTCCGTCTCCTGA+GGG | - | tig0027765:20522-20541 | Msa1435650:intron | 55.0% | |
| CTATGGCTCTCCGTCTCCTG+AGG | - | tig0027765:20521-20540 | Msa1435650:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0027765 | gene | 20372 | 20932 | 20372 | ID=Msa1435650;Name=Msa1435650 |
| tig0027765 | mRNA | 20372 | 20932 | 20372 | ID=Msa1435650-mRNA-1;Parent=Msa1435650;Name=Msa1435650-mRNA-1;_AED=0.30;_eAED=0.33;_QI=0|0|0|1|1|1|2|0|119 |
| tig0027765 | exon | 20648 | 20932 | 20648 | ID=Msa1435650-mRNA-1:exon:9818;Parent=Msa1435650-mRNA-1 |
| tig0027765 | exon | 20372 | 20446 | 20372 | ID=Msa1435650-mRNA-1:exon:9817;Parent=Msa1435650-mRNA-1 |
| tig0027765 | CDS | 20648 | 20932 | 20648 | ID=Msa1435650-mRNA-1:cds;Parent=Msa1435650-mRNA-1 |
| tig0027765 | CDS | 20372 | 20446 | 20372 | ID=Msa1435650-mRNA-1:cds;Parent=Msa1435650-mRNA-1 |
| Gene Sequence |
| Protein sequence |
