AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0918220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCCTTTATGCATATTCT+TGG 0.250199 6_2:+45443935 None:intergenic
AGACAAGAAAGTACACTTTA+TGG 0.268266 6_2:-45443891 Msa0918220:CDS
TTGTAATTTGTTTCAACTAA+TGG 0.274387 6_2:-45446801 Msa0918220:five_prime_UTR
AAGAATATGCATAAAGGAAT+TGG 0.305271 6_2:-45443933 Msa0918220:CDS
CACTTTATGGCATATGTTGA+TGG 0.413051 6_2:-45443878 Msa0918220:CDS
ACAACCAAGAATATGCATAA+AGG 0.458992 6_2:-45443939 Msa0918220:CDS
ATGGCATATGTTGATGGAAG+TGG 0.511028 6_2:-45443872 Msa0918220:CDS
TCGAGGACTGCATTATATGA+TGG 0.521822 6_2:-45446701 Msa0918220:exon
TCTTACGAAGGGATGATGGA+AGG 0.572073 6_2:-45446747 Msa0918220:five_prime_UTR
GACTGCATTATATGATGGAT+AGG 0.594900 6_2:-45446696 Msa0918220:exon
TTATATGATGGATAGGCCCA+AGG 0.631018 6_2:-45446689 Msa0918220:intron
TCTATAATAGCGACAACTCG+AGG 0.645172 6_2:-45446718 Msa0918220:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAATTATATCATATATTTT+AGG - chr6_2:45444966-45444985 Msa0918220:intron 10.0%
!!! ATATTTTTTCTTTGTTATAA+AGG - chr6_2:45446008-45446027 Msa0918220:intron 10.0%
!!! AGTGACAAATTTTTATTTTA+TGG - chr6_2:45444099-45444118 Msa0918220:intron 15.0%
!!! ATAATTTAGAGTTGTTATTT+TGG - chr6_2:45444251-45444270 Msa0918220:intron 15.0%
!!! AAACTTTACATAAAATTTTG+CGG + chr6_2:45445026-45445045 None:intergenic 15.0%
!!! AAAATTATTGTCTTTTTAAC+TGG - chr6_2:45445543-45445562 Msa0918220:intron 15.0%
!!! GTTTTTAACTTATTTTTGTA+AGG + chr6_2:45445789-45445808 None:intergenic 15.0%
!! GTTGAAACAAATTACAAAAA+AGG + chr6_2:45443851-45443870 None:intergenic 20.0%
!!! TTGTAATTTGTTTCAACTAA+TGG - chr6_2:45443854-45443873 Msa0918220:CDS 20.0%
!! ATTGAAGACATAATTAAGAA+TGG + chr6_2:45444010-45444029 None:intergenic 20.0%
!! TTCTTAATTATGTCTTCAAT+TGG - chr6_2:45444010-45444029 Msa0918220:intron 20.0%
!!! CAATCTAATAGACATTATTT+TGG - chr6_2:45444159-45444178 Msa0918220:intron 20.0%
!!! AGATGAGTTTATATTTTTCT+TGG - chr6_2:45444345-45444364 Msa0918220:intron 20.0%
!!! GATGAGTTTATATTTTTCTT+GGG - chr6_2:45444346-45444365 Msa0918220:intron 20.0%
!!! TATTGAATTATCTTGCTTTT+TGG - chr6_2:45444492-45444511 Msa0918220:intron 20.0%
!!! AAAATCAAGTTTTGTTTGAT+GGG - chr6_2:45444586-45444605 Msa0918220:intron 20.0%
!!! TATTTTTTCCCCTTATTTTA+TGG - chr6_2:45444657-45444676 Msa0918220:intron 20.0%
!! TAACAACAAAAATAAATACG+TGG + chr6_2:45444777-45444796 None:intergenic 20.0%
!! TGAAAGAAAAAAGATACAAT+TGG - chr6_2:45444932-45444951 Msa0918220:intron 20.0%
!! ATGCATAATTCAACAAAAAT+AGG + chr6_2:45445191-45445210 None:intergenic 20.0%
!! AAATAAGTTAAAAACCTTAG+TGG - chr6_2:45445794-45445813 Msa0918220:intron 20.0%
!! AATAAGTTAAAAACCTTAGT+GGG - chr6_2:45445795-45445814 Msa0918220:intron 20.0%
!!! TTAATTTGTGAGTATAAGTT+TGG - chr6_2:45445907-45445926 Msa0918220:intron 20.0%
!!! ATTTAAAATAACTACCAACA+GGG + chr6_2:45446176-45446195 None:intergenic 20.0%
!! TTAAAGTCTTTGTAAAATCA+AGG - chr6_2:45446248-45446267 Msa0918220:intron 20.0%
!! TGTAAGTAGAAAGTAAATAT+TGG - chr6_2:45446342-45446361 Msa0918220:intron 20.0%
!! GTAAGTAGAAAGTAAATATT+GGG - chr6_2:45446343-45446362 Msa0918220:intron 20.0%
!!! NNNGGTTTTTTGATTTTTCA+AGG - chr6_2:45446537-45446556 Msa0918220:intron 20.0%
!!! CAAAATCAAGTTTTGTTTGA+TGG - chr6_2:45444585-45444604 Msa0918220:intron 25.0%
! ATAAGGGGAAAAAATAACAT+AGG + chr6_2:45444653-45444672 None:intergenic 25.0%
! TTTGATGAGAAGATGAAATT+TGG - chr6_2:45444734-45444753 Msa0918220:intron 25.0%
! AACAAAAATAAATACGTGGA+GGG + chr6_2:45444773-45444792 None:intergenic 25.0%
!!! TTTTGTTGTGAGTTTATGTT+AGG - chr6_2:45444827-45444846 Msa0918220:intron 25.0%
!! ATTTTGTCTCATTCTGTTTT+TGG - chr6_2:45444856-45444875 Msa0918220:intron 25.0%
! ACATAATGATTAAAGAGCAA+AGG + chr6_2:45445054-45445073 None:intergenic 25.0%
!! CATTCCATAAGCATATATTT+TGG + chr6_2:45445234-45445253 None:intergenic 25.0%
!! AGGTAATTTTCTGCATAATT+TGG - chr6_2:45445307-45445326 Msa0918220:intron 25.0%
! AGAAGAAAATAAAACCGATA+CGG + chr6_2:45445484-45445503 None:intergenic 25.0%
!!! TGATTTTGCTTTGTTTTCTT+TGG - chr6_2:45445633-45445652 Msa0918220:intron 25.0%
!!! GATTTTGCTTTGTTTTCTTT+GGG - chr6_2:45445634-45445653 Msa0918220:intron 25.0%
!! TTTTATGTAGTGTCAACTAT+AGG - chr6_2:45445759-45445778 Msa0918220:intron 25.0%
! TTATCAAAGATCATATGCTA+TGG - chr6_2:45446051-45446070 Msa0918220:intron 25.0%
!! TTTAAAATAACTACCAACAG+GGG + chr6_2:45446175-45446194 None:intergenic 25.0%
!! CATTTAAAATAACTACCAAC+AGG + chr6_2:45446177-45446196 None:intergenic 25.0%
!!! TTGGGATTTTGAGTATATTT+TGG - chr6_2:45446361-45446380 Msa0918220:intron 25.0%
! ACCTTACAAATTGATCAAAA+TGG + chr6_2:45446387-45446406 None:intergenic 25.0%
! GTGTATTTATATATACGTTC+CGG - chr6_2:45446653-45446672 Msa0918220:intron 25.0%
!! TGTGATACCTATAATTTTCA+AGG + chr6_2:45446699-45446718 None:intergenic 25.0%
! AATTCCTTTATGCATATTCT+TGG + chr6_2:45446723-45446742 None:intergenic 25.0%
! AAGAATATGCATAAAGGAAT+TGG - chr6_2:45446722-45446741 Msa0918220:five_prime_UTR 25.0%
! TCAGAATTCTTTTCTTACGA+AGG - chr6_2:45443896-45443915 Msa0918220:CDS 30.0%
! CAGAATTCTTTTCTTACGAA+GGG - chr6_2:45443897-45443916 Msa0918220:CDS 30.0%
AAACAACCATTGTTTACCTT+GGG + chr6_2:45443985-45444004 None:intergenic 30.0%
TAAACAACCATTGTTTACCT+TGG + chr6_2:45443986-45444005 None:intergenic 30.0%
! TTTTATTCTACCATTTGCTC+TGG - chr6_2:45444410-45444429 Msa0918220:intron 30.0%
AAATTTAACACCACCAACAT+AGG - chr6_2:45444547-45444566 Msa0918220:intron 30.0%
!!! TTTTCCCCTTATTTTATGGA+TGG - chr6_2:45444661-45444680 Msa0918220:intron 30.0%
CAACAAAAATAAATACGTGG+AGG + chr6_2:45444774-45444793 None:intergenic 30.0%
! ACATAAAATTTTGCGGACAT+TGG + chr6_2:45445019-45445038 None:intergenic 30.0%
AGCTCCAAAATATATGCTTA+TGG - chr6_2:45445227-45445246 Msa0918220:intron 30.0%
TAAATAGTTAAGCACACATC+TGG + chr6_2:45445276-45445295 None:intergenic 30.0%
!! TTTTCTGCATAATTTAGAGC+TGG - chr6_2:45445346-45445365 Msa0918220:intron 30.0%
!!! CATAATTTAGAGCTGGTTTT+CGG - chr6_2:45445353-45445372 Msa0918220:intron 30.0%
! GATTGATTCTTATGTTCTCT+TGG - chr6_2:45445656-45445675 Msa0918220:intron 30.0%
!!! TCTCTTGGTTCTTATTTTCT+TGG - chr6_2:45445671-45445690 Msa0918220:intron 30.0%
!!! CTCTTGGTTCTTATTTTCTT+GGG - chr6_2:45445672-45445691 Msa0918220:intron 30.0%
CACATCAAGAAAGCAAATTT+AGG - chr6_2:45445835-45445854 Msa0918220:intron 30.0%
! CTTGTAGTCTTGAATTGATA+TGG + chr6_2:45446114-45446133 None:intergenic 30.0%
!! GCCATTTTGATCAATTTGTA+AGG - chr6_2:45446383-45446402 Msa0918220:intron 30.0%
AAGAAAGAACTTTCAAACAG+TGG + chr6_2:45446415-45446434 None:intergenic 30.0%
!! CTATAATTTTCAAGGCATGT+TGG + chr6_2:45446691-45446710 None:intergenic 30.0%
CAACATGCCTTGAAAATTAT+AGG - chr6_2:45446689-45446708 Msa0918220:intron 30.0%
ACAACCAAGAATATGCATAA+AGG - chr6_2:45446716-45446735 Msa0918220:five_prime_UTR 30.0%
! AGACAAGAAAGTACACTTTA+TGG - chr6_2:45446764-45446783 Msa0918220:five_prime_UTR 30.0%
!! GACTGCATTATATGATGGAT+AGG - chr6_2:45443959-45443978 Msa0918220:intron 35.0%
!!! AGACATTATTTTGGCTGAGT+AGG - chr6_2:45444168-45444187 Msa0918220:intron 35.0%
! GAAATCTATTGTGTTTTCCC+AGG - chr6_2:45444273-45444292 Msa0918220:intron 35.0%
! TTGTGTTTTCCCAGGTATTA+TGG - chr6_2:45444281-45444300 Msa0918220:intron 35.0%
GTTGTACAACAACAGTTCAT+AGG + chr6_2:45444307-45444326 None:intergenic 35.0%
AATCTTACTTGATGTCACTG+TGG - chr6_2:45444613-45444632 Msa0918220:intron 35.0%
ACCACCATCCATAAAATAAG+GGG + chr6_2:45444668-45444687 None:intergenic 35.0%
CACCACCATCCATAAAATAA+GGG + chr6_2:45444669-45444688 None:intergenic 35.0%
ACACCACCATCCATAAAATA+AGG + chr6_2:45444670-45444689 None:intergenic 35.0%
!! TTATTTTATGGATGGTGGTG+TGG - chr6_2:45444669-45444688 Msa0918220:intron 35.0%
!!! TGTCTCATTCTGTTTTTGGT+AGG - chr6_2:45444860-45444879 Msa0918220:intron 35.0%
! CAATCAGAAGCATGAAATAG+AGG + chr6_2:45445160-45445179 None:intergenic 35.0%
!!! TTTTGGAGCTATGCTAACTA+AGG + chr6_2:45445217-45445236 None:intergenic 35.0%
! TGTGCTTAACTATTTAGAGC+TGG - chr6_2:45445280-45445299 Msa0918220:intron 35.0%
! AACTATTTAGAGCTGGTTGA+AGG - chr6_2:45445287-45445306 Msa0918220:intron 35.0%
CACAGAGAAAAATCAGAACA+CGG + chr6_2:45445450-45445469 None:intergenic 35.0%
CAAACTACAACAACCTATTC+TGG + chr6_2:45445598-45445617 None:intergenic 35.0%
CAGAATAGGTTGTTGTAGTT+TGG - chr6_2:45445596-45445615 Msa0918220:intron 35.0%
TCACAACAATTAACCCACTA+AGG + chr6_2:45445811-45445830 None:intergenic 35.0%
CAGTGAAAGTCAACTGATAA+AGG - chr6_2:45445955-45445974 Msa0918220:intron 35.0%
! TGGATTTTGAACAACTCAGT+TGG - chr6_2:45446071-45446090 Msa0918220:intron 35.0%
TTCTGTTGTTGCACATGATT+TGG - chr6_2:45446620-45446639 Msa0918220:intron 35.0%
TTTATATATACGTTCCGGAC+TGG - chr6_2:45446658-45446677 Msa0918220:intron 35.0%
CACTTTATGGCATATGTTGA+TGG - chr6_2:45446777-45446796 Msa0918220:five_prime_UTR 35.0%
! CTTTTCTTACGAAGGGATGA+TGG - chr6_2:45443904-45443923 Msa0918220:CDS 40.0%
TCTATAATAGCGACAACTCG+AGG - chr6_2:45443937-45443956 Msa0918220:CDS 40.0%
TCGAGGACTGCATTATATGA+TGG - chr6_2:45443954-45443973 Msa0918220:intron 40.0%
!! TTATATGATGGATAGGCCCA+AGG - chr6_2:45443966-45443985 Msa0918220:intron 40.0%
! ATATCGAAGTGTTAGCGTGA+CGG - chr6_2:45444200-45444219 Msa0918220:intron 40.0%
GTTCATAGGCCATAATACCT+GGG + chr6_2:45444293-45444312 None:intergenic 40.0%
AGTTCATAGGCCATAATACC+TGG + chr6_2:45444294-45444313 None:intergenic 40.0%
!!! TCCCCTTATTTTATGGATGG+TGG - chr6_2:45444664-45444683 Msa0918220:intron 40.0%
AGAAAAATCAGAACACGGTG+AGG + chr6_2:45445445-45445464 None:intergenic 40.0%
! CCTATTCTGGAAAAAGCAAC+AGG + chr6_2:45445585-45445604 None:intergenic 40.0%
!!! CCTGTTGCTTTTTCCAGAAT+AGG - chr6_2:45445582-45445601 Msa0918220:intron 40.0%
TGAAATAAATGTGCCCCTGT+TGG - chr6_2:45446159-45446178 Msa0918220:intron 40.0%
! ATGGCATATGTTGATGGAAG+TGG - chr6_2:45446783-45446802 Msa0918220:five_prime_UTR 40.0%
!! TCTTACGAAGGGATGATGGA+AGG - chr6_2:45443908-45443927 Msa0918220:CDS 45.0%
GATAGGCCCAAGGTAAACAA+TGG - chr6_2:45443976-45443995 Msa0918220:intron 45.0%
TAAGCTGCAACTGGATCAAG+TGG + chr6_2:45444385-45444404 None:intergenic 45.0%
TTGATCCAGTTGCAGCTTAG+CGG - chr6_2:45444386-45444405 Msa0918220:intron 45.0%
AACTTGCAGACCAGAGCAAA+TGG + chr6_2:45444423-45444442 None:intergenic 45.0%
ACTCCTGCTTCATCCTATGT+TGG + chr6_2:45444563-45444582 None:intergenic 45.0%
! CATAATTTGGAGCGAGATCG+AGG - chr6_2:45445320-45445339 Msa0918220:intron 45.0%
! GTAGTGCAGCAGCAATGTTT+TGG - chr6_2:45445389-45445408 Msa0918220:intron 45.0%
! GTCCTTGTCTTCATGCGTTT+TGG - chr6_2:45445508-45445527 Msa0918220:intron 45.0%
AGCCAAAACGCATGAAGACA+AGG + chr6_2:45445513-45445532 None:intergenic 45.0%
ATGCCCATTGAAGAACTGCT+TGG - chr6_2:45445867-45445886 Msa0918220:intron 45.0%
TGCCCATTGAAGAACTGCTT+GGG - chr6_2:45445868-45445887 Msa0918220:intron 45.0%
GATATGGAATGAGCCATGCT+TGG + chr6_2:45446098-45446117 None:intergenic 45.0%
! TGCACATGATTTGGCAGATG+TGG - chr6_2:45446629-45446648 Msa0918220:intron 45.0%
!!! TTTTAAAATTATATTTATGT+TGG - chr6_2:45444462-45444481 Msa0918220:intron 5.0%
GCAACTGGATCAAGTGGAGA+CGG + chr6_2:45444379-45444398 None:intergenic 50.0%
AAAAGCCGCTAAGCTGCAAC+TGG + chr6_2:45444394-45444413 None:intergenic 50.0%
CCTGCTTCATCCTATGTTGG+TGG + chr6_2:45444560-45444579 None:intergenic 50.0%
CCACCAACATAGGATGAAGC+AGG - chr6_2:45444557-45444576 Msa0918220:intron 50.0%
CTTGATGTCACTGTGGTTGC+AGG - chr6_2:45444620-45444639 Msa0918220:intron 50.0%
! TTATGGATGGTGGTGTGGCT+AGG - chr6_2:45444674-45444693 Msa0918220:intron 50.0%
!!! TAGAGCTGGTTTTCGGCATG+TGG - chr6_2:45445360-45445379 Msa0918220:intron 50.0%
!! GGTTTTCGGCATGTGGACTA+AGG - chr6_2:45445367-45445386 Msa0918220:intron 50.0%
GTATTGCAGCTGCTACGTAG+TGG - chr6_2:45445411-45445430 Msa0918220:intron 50.0%
TAAAACCGATACGGTGCAGC+TGG + chr6_2:45445475-45445494 None:intergenic 50.0%
GCCCATTGAAGAACTGCTTG+GGG - chr6_2:45445869-45445888 Msa0918220:intron 50.0%
GCCCCAAGCAGTTCTTCAAT+GGG + chr6_2:45445873-45445892 None:intergenic 50.0%
AGCCCCAAGCAGTTCTTCAA+TGG + chr6_2:45445874-45445893 None:intergenic 50.0%
CAACTCAGTTGGACCAAGCA+TGG - chr6_2:45446082-45446101 Msa0918220:intron 50.0%
!! ATATACGTTCCGGACTGGTG+TGG - chr6_2:45446663-45446682 Msa0918220:intron 50.0%
! TCGAAGTGTTAGCGTGACGG+TGG - chr6_2:45444203-45444222 Msa0918220:intron 55.0%
GTGTTCCAGCTGCACCGTAT+CGG - chr6_2:45445467-45445486 Msa0918220:intron 55.0%
ATGTTGGCGCCACACCAGTC+CGG + chr6_2:45446675-45446694 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 45443844 45446833 45443844 ID=Msa0918220;Name=Msa0918220
chr6_2 mRNA 45443844 45446833 45443844 ID=Msa0918220-mRNA-1;Parent=Msa0918220;Name=Msa0918220-mRNA-1;_AED=0.40;_eAED=0.68;_QI=126|0|0.5|1|0|0|2|0|47
chr6_2 exon 45443844 45443966 45443844 ID=Msa0918220-mRNA-1:exon:7152;Parent=Msa0918220-mRNA-1
chr6_2 exon 45446690 45446833 45446690 ID=Msa0918220-mRNA-1:exon:7151;Parent=Msa0918220-mRNA-1
chr6_2 five_prime_UTR 45446708 45446833 45446708 ID=Msa0918220-mRNA-1:five_prime_utr;Parent=Msa0918220-mRNA-1
chr6_2 CDS 45446690 45446707 45446690 ID=Msa0918220-mRNA-1:cds;Parent=Msa0918220-mRNA-1
chr6_2 CDS 45443844 45443966 45443844 ID=Msa0918220-mRNA-1:cds;Parent=Msa0918220-mRNA-1
Gene Sequence

>Msa0918220

ATGATGGATAGGCCCAAGGTATCACAACCAAGAATATGCATAAAGGAATTGGAAGCGCTAAACACTTTAACAGACAAGAAAGTACACTTTATGGCATATGTTGATGGAAGTGGTAGAGATTTGATGATCGTTAAAACAACT

Protein sequence

>Msa0918220

MMDRPKVSQPRICIKELEALNTLTDKKVHFMAYVDGSGRDLMIVKTT