AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1079180


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCAGGTCAAGGCTGATTT+TGG 0.138857 7_2:-78224922 Msa1079180:intron
AACCCGCATTTCTAACTTAA+TGG 0.155002 7_2:+78224848 None:intergenic
TACTGGTGATGTTTCTAAAA+AGG 0.173626 7_2:-78225147 Msa1079180:five_prime_UTR
TCGGATTAACCAACACAATT+AGG 0.275265 7_2:-78225213 Msa1079180:five_prime_UTR
TGAGTTGAGAAGGTTGAATA+AGG 0.323601 7_2:-78225414 Msa1079180:five_prime_UTR
CACTTGTAGGCTCTCTTCTA+TGG 0.338739 7_2:-78225366 Msa1079180:five_prime_UTR
ATTTCTAACTTAATGGTACT+TGG 0.426986 7_2:+78224855 None:intergenic
TTAACTTAAATCATACTGAT+AGG 0.430639 7_2:-78225038 Msa1079180:CDS
AACCAACACAATTAGGCTCT+TGG 0.438251 7_2:-78225206 Msa1079180:five_prime_UTR
GATAAGAGGAAGCGGTGAAG+GGG 0.453973 7_2:-78225481 None:intergenic
TACCATTAAGTTAGAAATGC+GGG 0.455867 7_2:-78224850 Msa1079180:exon
TTGTAATAGAAGATATGAAT+AGG 0.461497 7_2:-78225242 Msa1079180:five_prime_UTR
AGCCTTGCTCTGCAAATACT+TGG 0.488191 7_2:+78224879 None:intergenic
AGATAAGAGGAAGCGGTGAA+GGG 0.491215 7_2:-78225482 None:intergenic
AGATATGAATAGGCTCTCTT+CGG 0.510356 7_2:-78225232 Msa1079180:five_prime_UTR
AGAAGAGGAACCTCACTTGT+AGG 0.517175 7_2:-78225379 Msa1079180:five_prime_UTR
GTCCAAGTATTTGCAGAGCA+AGG 0.527011 7_2:-78224881 Msa1079180:CDS
AGCCAAGAGCCTAATTGTGT+TGG 0.531992 7_2:+78225204 None:intergenic
TTACTGAGTTGGACTTAAAG+TGG 0.536061 7_2:-78225094 Msa1079180:five_prime_UTR
ATAAGAGGAAGCGGTGAAGG+GGG 0.538898 7_2:-78225480 None:intergenic
TTGAAGTTGTTGAGTTGAGA+AGG 0.559117 7_2:-78225424 Msa1079180:five_prime_UTR
TGTATGCTATCTTACTGAGT+TGG 0.580470 7_2:-78225105 Msa1079180:five_prime_UTR
AGGAGCTGCATATGTAGAAG+AGG 0.591272 7_2:-78225394 Msa1079180:five_prime_UTR
ATGATTTAAGTTAATAACCA+TGG 0.599391 7_2:+78225047 None:intergenic
GTACCATTAAGTTAGAAATG+CGG 0.611734 7_2:-78224851 Msa1079180:exon
TCATACTGATAGGACAGTGA+TGG 0.631868 7_2:-78225028 Msa1079180:intron
TAGAAGAGAGCCTACAAGTG+AGG 0.633933 7_2:+78225369 None:intergenic
TAGTTGTGCCTGCCAAAACA+CGG 0.641537 7_2:+78225168 None:intergenic
TAACTTGCTGAGAAATACCA+TGG 0.684621 7_2:-78225064 Msa1079180:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTAATAGAAGATATGAAT+AGG - chr7_2:78225015-78225034 Msa1079180:intron 20.0%
!!! ATGATTTAAGTTAATAACCA+TGG + chr7_2:78225213-78225232 None:intergenic 20.0%
!! TTAACTTAAATCATACTGAT+AGG - chr7_2:78225219-78225238 Msa1079180:five_prime_UTR 20.0%
!! TGACATAATTTCTTTTCTCA+TGG - chr7_2:78224983-78225002 Msa1079180:intron 25.0%
!! GACATAATTTCTTTTCTCAT+GGG - chr7_2:78224984-78225003 Msa1079180:intron 25.0%
! ATTTCTAACTTAATGGTACT+TGG + chr7_2:78225405-78225424 None:intergenic 25.0%
! TACTGGTGATGTTTCTAAAA+AGG - chr7_2:78225110-78225129 Msa1079180:five_prime_UTR 30.0%
! ACTGGTGATGTTTCTAAAAA+GGG - chr7_2:78225111-78225130 Msa1079180:five_prime_UTR 30.0%
!! AGTACTTGATAACCTCATTT+TGG + chr7_2:78225288-78225307 None:intergenic 30.0%
GTACCATTAAGTTAGAAATG+CGG - chr7_2:78225406-78225425 Msa1079180:five_prime_UTR 30.0%
TACCATTAAGTTAGAAATGC+GGG - chr7_2:78225407-78225426 Msa1079180:five_prime_UTR 30.0%
TAAAAAGTAAACAAGCAGCA+AGG + chr7_2:78225447-78225466 None:intergenic 30.0%
!!! TTGCTGCTTGTTTACTTTTT+AGG - chr7_2:78225446-78225465 Msa1079180:five_prime_UTR 30.0%
!! TTGAAGTTGTTGAGTTGAGA+AGG - chr7_2:78224833-78224852 Msa1079180:three_prime_UTR 35.0%
! TGAGTTGAGAAGGTTGAATA+AGG - chr7_2:78224843-78224862 Msa1079180:three_prime_UTR 35.0%
AGATATGAATAGGCTCTCTT+CGG - chr7_2:78225025-78225044 Msa1079180:intron 35.0%
TCGGATTAACCAACACAATT+AGG - chr7_2:78225044-78225063 Msa1079180:CDS 35.0%
!! TTTTGATTGATGCCGTGTTT+TGG - chr7_2:78225077-78225096 Msa1079180:five_prime_UTR 35.0%
! ATGTTTCTAAAAAGGGTCGA+TGG - chr7_2:78225118-78225137 Msa1079180:five_prime_UTR 35.0%
TGTATGCTATCTTACTGAGT+TGG - chr7_2:78225152-78225171 Msa1079180:five_prime_UTR 35.0%
! TTACTGAGTTGGACTTAAAG+TGG - chr7_2:78225163-78225182 Msa1079180:five_prime_UTR 35.0%
!! TAACTTGCTGAGAAATACCA+TGG - chr7_2:78225193-78225212 Msa1079180:five_prime_UTR 35.0%
TTTGGACAGAGTAAACTTCA+AGG + chr7_2:78225270-78225289 None:intergenic 35.0%
AGTTTACTCTGTCCAAAATG+AGG - chr7_2:78225273-78225292 Msa1079180:five_prime_UTR 35.0%
AACCCGCATTTCTAACTTAA+TGG + chr7_2:78225412-78225431 None:intergenic 35.0%
AACCAACACAATTAGGCTCT+TGG - chr7_2:78225051-78225070 Msa1079180:exon 40.0%
TCATACTGATAGGACAGTGA+TGG - chr7_2:78225229-78225248 Msa1079180:five_prime_UTR 40.0%
CAGTAGAGGATAGGCAAATT+GGG + chr7_2:78225318-78225337 None:intergenic 40.0%
AGGAGCTGCATATGTAGAAG+AGG - chr7_2:78224863-78224882 Msa1079180:exon 45.0%
AGAAGAGGAACCTCACTTGT+AGG - chr7_2:78224878-78224897 Msa1079180:CDS 45.0%
TAGAAGAGAGCCTACAAGTG+AGG + chr7_2:78224891-78224910 None:intergenic 45.0%
CACTTGTAGGCTCTCTTCTA+TGG - chr7_2:78224891-78224910 Msa1079180:CDS 45.0%
AGCCAAGAGCCTAATTGTGT+TGG + chr7_2:78225056-78225075 None:intergenic 45.0%
TAGTTGTGCCTGCCAAAACA+CGG + chr7_2:78225092-78225111 None:intergenic 45.0%
GCAGTAGAGGATAGGCAAAT+TGG + chr7_2:78225319-78225338 None:intergenic 45.0%
ATTTGCCTATCCTCTACTGC+AGG - chr7_2:78225319-78225338 Msa1079180:five_prime_UTR 45.0%
!! TGCAGGTCAAGGCTGATTTT+GGG - chr7_2:78225336-78225355 Msa1079180:five_prime_UTR 45.0%
GTCCAAGTATTTGCAGAGCA+AGG - chr7_2:78225376-78225395 Msa1079180:five_prime_UTR 45.0%
AGCCTTGCTCTGCAAATACT+TGG + chr7_2:78225381-78225400 None:intergenic 45.0%
!!! GATTGATGCCGTGTTTTGGC+AGG - chr7_2:78225081-78225100 Msa1079180:five_prime_UTR 50.0%
!! GTTTTGGCAGGCACAACTAC+TGG - chr7_2:78225093-78225112 Msa1079180:five_prime_UTR 50.0%
CTTGACCTGCAGTAGAGGAT+AGG + chr7_2:78225327-78225346 None:intergenic 50.0%
CTATCCTCTACTGCAGGTCA+AGG - chr7_2:78225325-78225344 Msa1079180:five_prime_UTR 50.0%
!! CTGCAGGTCAAGGCTGATTT+TGG - chr7_2:78225335-78225354 Msa1079180:five_prime_UTR 50.0%
TCAGCCTTGACCTGCAGTAG+AGG + chr7_2:78225332-78225351 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 78224790 78225489 78224790 ID=Msa1079180;Name=Msa1079180
chr7_2 mRNA 78224790 78225489 78224790 ID=Msa1079180-mRNA-1;Parent=Msa1079180;Name=Msa1079180-mRNA-1;_AED=0.28;_eAED=0.28;_QI=422|1|1|1|1|1|2|74|37
chr7_2 exon 78224790 78224938 78224790 ID=Msa1079180-mRNA-1:exon:19481;Parent=Msa1079180-mRNA-1
chr7_2 exon 78225029 78225489 78225029 ID=Msa1079180-mRNA-1:exon:19480;Parent=Msa1079180-mRNA-1
chr7_2 five_prime_UTR 78225068 78225489 78225068 ID=Msa1079180-mRNA-1:five_prime_utr;Parent=Msa1079180-mRNA-1
chr7_2 CDS 78225029 78225067 78225029 ID=Msa1079180-mRNA-1:cds;Parent=Msa1079180-mRNA-1
chr7_2 CDS 78224864 78224938 78224864 ID=Msa1079180-mRNA-1:cds;Parent=Msa1079180-mRNA-1
chr7_2 three_prime_UTR 78224790 78224863 78224790 ID=Msa1079180-mRNA-1:three_prime_utr;Parent=Msa1079180-mRNA-1
Gene Sequence

>Msa1079180

ATGGTTATTAACTTAAATCATACTGATAGGACAGTGATGGTCAAGGCTGATTTTGGGTCTGTTATTGATGCTTTGTCCAAGTATTTGCAGAGCAAGGCTGCCAAGTACCATTAA

Protein sequence

>Msa1079180

MVINLNHTDRTVMVKADFGSVIDALSKYLQSKAAKYH*