AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006665

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006665.t1 MTR_4g079770 95.072 345 15 1 1 343 1 345 0.0 686
MS.gene006665.t1 MTR_4g079470 96.605 324 11 0 20 343 22 345 0.0 660
MS.gene006665.t1 MTR_4g079800 92.593 324 24 0 20 343 22 345 0.0 633
MS.gene006665.t1 MTR_5g022560 88.116 345 38 3 1 343 1 344 0.0 632
MS.gene006665.t1 MTR_4g107930 87.572 346 39 3 1 343 1 345 0.0 628
MS.gene006665.t1 MTR_4g047610 88.406 345 36 3 1 343 1 343 0.0 615
MS.gene006665.t1 MTR_2g075830 76.232 345 76 5 1 343 1 341 0.0 521
MS.gene006665.t1 MTR_4g080700 65.217 322 104 7 24 343 25 340 2.64e-151 429
MS.gene006665.t1 MTR_4g080730 64.907 322 105 6 24 343 25 340 1.19e-150 427
MS.gene006665.t1 MTR_4g080360 53.468 346 154 5 1 343 1 342 6.89e-131 377
MS.gene006665.t1 MTR_4g104370 57.522 339 133 9 9 341 9 342 7.70e-131 378
MS.gene006665.t1 MTR_2g083930 52.616 344 147 6 5 341 8 342 3.33e-127 368
MS.gene006665.t1 MTR_3g075390 53.550 338 147 7 9 341 11 343 1.03e-125 365
MS.gene006665.t1 MTR_4g079440 54.808 312 134 4 31 341 38 343 1.97e-121 353
MS.gene006665.t1 MTR_3g116080 52.199 341 148 7 10 342 14 347 1.73e-120 351
MS.gene006665.t1 MTR_1g107550 54.259 317 137 5 30 341 41 354 3.73e-118 350
MS.gene006665.t1 MTR_7g078600 57.097 310 121 7 34 341 37 336 8.69e-118 344
MS.gene006665.t1 MTR_2g090890 49.263 339 160 5 9 342 5 336 1.34e-116 341
MS.gene006665.t1 MTR_7g079230 51.917 339 150 8 8 341 4 334 4.25e-116 340
MS.gene006665.t1 MTR_4g094918 48.980 343 161 8 9 342 4 341 4.03e-114 335
MS.gene006665.t1 MTR_3g102220 48.229 367 157 10 1 341 1 360 2.64e-112 335
MS.gene006665.t1 MTR_1g018840 51.682 327 138 7 25 341 44 360 1.21e-111 332
MS.gene006665.t1 MTR_1g018840 51.524 328 139 7 24 341 43 360 1.65e-111 333
MS.gene006665.t1 MTR_3g112430 50.161 311 141 5 40 342 37 341 1.92e-102 308
MS.gene006665.t1 MTR_2g015850 54.828 290 123 6 55 341 78 362 2.66e-99 298
MS.gene006665.t1 MTR_2g016180 43.931 346 173 9 10 342 18 355 1.29e-89 277
MS.gene006665.t1 MTR_2g016270 44.444 342 162 10 10 342 18 340 6.42e-88 272
MS.gene006665.t1 MTR_2g067900 44.375 320 155 12 38 342 15 326 2.87e-77 240
MS.gene006665.t1 MTR_2g067560 44.548 321 146 13 38 337 15 324 1.26e-72 242
MS.gene006665.t1 MTR_4g125300 41.234 308 163 10 41 342 53 348 1.26e-69 221
MS.gene006665.t1 MTR_2g064475 39.429 350 154 16 38 342 15 351 6.34e-69 219
MS.gene006665.t1 MTR_2g015920 39.490 314 176 10 37 342 51 358 2.65e-67 216
MS.gene006665.t1 MTR_2g066980 34.831 356 171 11 8 342 3 318 5.24e-67 214
MS.gene006665.t1 MTR_4g065077 37.963 324 183 9 29 342 41 356 6.22e-67 215
MS.gene006665.t1 MTR_4g071060 37.006 354 184 12 10 334 5 348 7.34e-66 212
MS.gene006665.t1 MTR_7g078630 45.487 277 110 5 37 302 704 950 8.15e-65 221
MS.gene006665.t1 MTR_1g023210 38.602 329 160 12 31 334 41 352 4.98e-64 207
MS.gene006665.t1 MTR_8g086470 36.957 322 173 9 29 342 57 356 5.32e-62 202
MS.gene006665.t1 MTR_1g023810 45.022 231 118 6 113 340 37 261 1.54e-61 198
MS.gene006665.t1 MTR_2g015930 39.252 321 168 12 37 342 50 358 1.93e-61 201
MS.gene006665.t1 MTR_1g094135 36.499 337 177 12 22 334 35 358 2.61e-57 190
MS.gene006665.t1 MTR_4g072790 34.343 297 158 8 41 334 50 312 4.75e-45 157
MS.gene006665.t1 MTR_4g072340 34.219 301 157 10 41 334 50 316 4.01e-42 149
MS.gene006665.t1 MTR_4g073660 33.887 301 158 10 41 334 49 315 5.21e-42 149
MS.gene006665.t1 MTR_7g078580 63.265 98 35 1 244 341 4 100 4.61e-41 140
MS.gene006665.t1 MTR_2g015840 49.367 158 74 5 6 160 2 156 1.09e-40 144
MS.gene006665.t1 MTR_2g015840 55.357 112 49 1 231 342 161 271 4.97e-36 132
MS.gene006665.t1 MTR_7g079130 68.889 90 28 0 252 341 7 96 3.18e-40 137
MS.gene006665.t1 MTR_1g025450 38.503 187 90 7 113 295 43 208 1.49e-32 127
MS.gene006665.t1 MTR_7g078310 45.000 140 67 3 82 218 115 247 2.55e-27 108
MS.gene006665.t1 MTR_2g016380 47.107 121 57 4 226 342 18 135 3.69e-27 107
MS.gene006665.t1 MTR_7g111060 28.889 225 132 7 131 334 113 330 1.60e-22 97.1
MS.gene006665.t1 MTR_7g079260 59.722 72 13 1 268 339 7 62 2.68e-22 90.5
MS.gene006665.t1 MTR_7g111050 29.787 235 135 8 131 342 110 337 6.42e-22 95.5
MS.gene006665.t1 MTR_3g029560 27.888 251 146 9 98 342 16 237 4.64e-21 90.9
MS.gene006665.t1 MTR_8g087510 41.985 131 69 5 166 294 23 148 8.93e-21 88.6
MS.gene006665.t1 MTR_2g047960 48.235 85 43 1 134 218 7 90 7.93e-18 78.2
MS.gene006665.t1 MTR_3g082370 26.087 207 133 5 130 321 5 206 2.58e-17 80.5
MS.gene006665.t1 MTR_7g111050 27.692 195 117 6 131 304 105 296 1.07e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006665.t1 AT5G45890 55.233 344 145 4 6 342 4 345 1.02e-136 392
MS.gene006665.t1 AT5G50260 54.335 346 134 8 11 341 5 341 9.41e-130 375
MS.gene006665.t1 AT3G48340 52.616 344 145 7 11 341 4 342 1.86e-122 357
MS.gene006665.t1 AT2G34080 51.170 342 156 6 9 343 8 345 4.33e-121 353
MS.gene006665.t1 AT4G35350 51.020 343 152 8 9 342 17 352 1.40e-117 344
MS.gene006665.t1 AT3G19390 50.000 350 159 7 3 342 2 345 1.60e-116 345
MS.gene006665.t1 AT3G48350 51.009 347 153 8 9 342 1 343 3.66e-116 341
MS.gene006665.t1 AT5G43060 52.761 326 140 6 23 341 34 352 3.93e-115 342
MS.gene006665.t1 AT2G27420 48.442 353 159 7 9 343 1 348 7.86e-115 337
MS.gene006665.t1 AT3G49340 51.988 327 145 6 24 343 20 341 9.63e-115 336
MS.gene006665.t1 AT1G06260 52.353 340 149 7 8 341 9 341 4.95e-114 335
MS.gene006665.t1 AT1G29090 46.459 353 172 6 4 343 7 355 2.97e-111 328
MS.gene006665.t1 AT4G36880 50.459 327 146 7 27 342 40 361 4.44e-111 328
MS.gene006665.t1 AT3G19400 50.814 307 146 5 39 342 44 348 1.83e-110 326
MS.gene006665.t1 AT1G20850 50.467 321 152 5 25 342 37 353 2.51e-110 325
MS.gene006665.t1 AT1G47128 51.780 309 136 7 39 341 50 351 7.64e-110 328
MS.gene006665.t1 AT1G29080 46.064 343 163 7 14 343 13 346 3.57e-107 317
MS.gene006665.t1 AT1G09850 50.654 306 147 4 37 341 30 332 1.99e-102 308
MS.gene006665.t1 AT4G23520 48.544 309 144 8 40 341 48 348 4.47e-100 300
MS.gene006665.t1 AT4G11310 44.286 350 169 9 9 341 11 351 2.45e-98 295
MS.gene006665.t1 AT4G11320 46.349 315 143 7 40 341 57 358 4.91e-97 292
MS.gene006665.t1 AT4G11320 46.349 315 143 7 40 341 57 358 4.91e-97 292
MS.gene006665.t1 AT1G29110 41.108 343 166 7 14 342 13 333 7.21e-88 268
MS.gene006665.t1 AT3G43960 44.277 332 174 8 15 341 20 345 4.52e-85 262
MS.gene006665.t1 AT4G35350 48.375 277 127 8 9 276 17 286 1.23e-83 255
MS.gene006665.t1 AT3G19400 48.963 241 119 4 39 276 44 283 1.44e-76 237
MS.gene006665.t1 AT3G48350 47.331 281 131 8 9 276 1 277 1.48e-76 237
MS.gene006665.t1 AT5G60360 43.648 307 157 9 41 342 61 356 4.05e-76 238
MS.gene006665.t1 AT5G60360 43.322 307 157 10 41 342 61 355 1.23e-73 232
MS.gene006665.t1 AT3G45310 39.368 348 182 11 1 343 34 357 2.27e-73 231
MS.gene006665.t1 AT5G60360 42.953 298 154 9 41 333 61 347 8.22e-72 227
MS.gene006665.t1 AT3G45310 39.080 348 182 12 1 343 34 356 8.54e-71 224
MS.gene006665.t1 AT2G21430 37.188 320 171 9 30 334 45 349 1.06e-63 206
MS.gene006665.t1 AT4G39090 38.344 326 163 12 28 334 46 352 2.42e-63 206
MS.gene006665.t1 AT3G54940 38.750 320 158 10 38 334 50 354 1.02e-60 199
MS.gene006665.t1 AT4G16190 38.907 311 158 11 44 334 60 358 4.73e-60 197
MS.gene006665.t1 AT3G54940 39.056 233 124 5 38 258 50 276 3.43e-45 156
MS.gene006665.t1 AT1G02305 29.778 225 130 7 131 334 116 333 6.81e-24 101
MS.gene006665.t1 AT4G01610 27.063 303 178 12 65 338 46 334 4.09e-21 93.2
MS.gene006665.t1 AT1G02300 29.384 211 123 6 144 334 146 350 6.82e-20 90.1
MS.gene006665.t1 AT4G01610 26.733 303 179 12 65 338 46 334 8.34e-20 89.7
MS.gene006665.t1 AT2G22160 40.217 92 52 2 54 144 9 98 1.17e-14 69.7

Find 95 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAAAAGTGGTGTCTTTAC+TGG 0.245613 4.1:+26646512 MS.gene006665:CDS
ACTTCCCAGTACTCAACTTA+TGG 0.259340 4.1:-26646184 None:intergenic
TGACATGTTATGTTGATTAT+TGG 0.261556 4.1:-26645607 None:intergenic
GCATCAATAGCTACGGAAAT+TGG 0.285597 4.1:-26646467 None:intergenic
CTGCAGTGCCTGCTCATTGT+TGG 0.329361 4.1:-26646426 None:intergenic
AGTTCCGTCAACACCTTGAT+AGG 0.330791 4.1:-26646342 None:intergenic
TGGGAAGTTGGTCTCTTTAT+CGG 0.332823 4.1:+26646199 MS.gene006665:CDS
CTTGTTATTGCCAGCATTGT+TGG 0.332876 4.1:-26645817 None:intergenic
AATAACAAGTTATACAAATT+AGG 0.348584 4.1:+26645831 MS.gene006665:CDS
GAATTCATGGGGAACTGATT+GGG 0.351922 4.1:+26646619 MS.gene006665:CDS
TCACTATGGCAAAGTCTATA+AGG 0.352872 4.1:+26645731 MS.gene006665:CDS
ATCCATGCAGTTACCATTAC+CGG 0.356686 4.1:+26646386 MS.gene006665:CDS
AAATTCATCATTCAAAATCA+TGG 0.364764 4.1:+26646296 MS.gene006665:CDS
ATTGGTTGGTTAAGAATTCA+TGG 0.370703 4.1:+26646606 MS.gene006665:CDS
AGGTTGTGAGGGTGGCCTTA+TGG 0.393598 4.1:+26646262 MS.gene006665:CDS
CATTGACCTTGGTTCTTCAC+TGG 0.396716 4.1:-26646014 None:intergenic
AGAATTCATGGGGAACTGAT+TGG 0.403396 4.1:+26646618 MS.gene006665:CDS
GGAATCCATAAGTTGAGTAC+TGG 0.422547 4.1:+26646179 MS.gene006665:CDS
AGTAGTTGTCTTTGTGATTG+AGG 0.437424 4.1:-26645928 None:intergenic
AGTGCCTGCTCATTGTTGGC+AGG 0.443934 4.1:-26646422 None:intergenic
TTTCTCCAATCCACTGCTGA+TGG 0.445961 4.1:-26645975 None:intergenic
ACTCAACTTATGGATTCCTT+CGG 0.446108 4.1:-26646174 None:intergenic
GGTGTCACTGCTGTAGGTTA+TGG 0.447580 4.1:+26646560 MS.gene006665:CDS
ATTCTTAACCAACCAATACT+TGG 0.453395 4.1:-26646600 None:intergenic
AGGATATATTAGGATGCAAA+GGG 0.460167 4.1:+26646649 MS.gene006665:CDS
CATACATGGAGTGGTCATCT+TGG 0.465658 4.1:-26645686 None:intergenic
GACTTTCAGTTCTACAAAAG+TGG 0.468517 4.1:+26646500 MS.gene006665:CDS
TTGCTCATGCTTCTCATACA+TGG 0.469927 4.1:-26645700 None:intergenic
GGAACTGATTGGGGTGAAGA+AGG 0.474210 4.1:+26646629 MS.gene006665:CDS
TAATCAACATAACATGTCAT+TGG 0.474648 4.1:+26645611 MS.gene006665:CDS
AAGTTTGGAACTTAAGCTGT+AGG 0.482556 4.1:-26646722 None:intergenic
TGCATCTAGAAACAAATTCA+AGG 0.484430 4.1:+26645893 MS.gene006665:CDS
GATGCTGCTCAAGGACTTTG+TGG 0.489207 4.1:+26646677 MS.gene006665:CDS
GGGGTGAAGAAGGATATATT+AGG 0.489294 4.1:+26646639 MS.gene006665:CDS
TCTATAAGGATTCTCAAGAA+AGG 0.490614 4.1:+26645745 MS.gene006665:CDS
AAGAAAGGGAGAACCGTCTC+AGG 0.495449 4.1:+26645760 MS.gene006665:CDS
GAGCAATGGATGAGTCACTA+TGG 0.497756 4.1:+26645717 MS.gene006665:CDS
TACCCCTATCAAGGTGTTGA+CGG 0.498791 4.1:+26646338 MS.gene006665:CDS
GACATCTTCATACCCGGTAA+TGG 0.502057 4.1:-26646399 None:intergenic
CTATAAGGATTCTCAAGAAA+GGG 0.505602 4.1:+26645746 MS.gene006665:CDS
ATTTGAAAGCATCATCCATA+AGG 0.506586 4.1:-26646277 None:intergenic
GCAGTGGATTGGAGAAAGAA+AGG 0.507676 4.1:+26645981 MS.gene006665:CDS
AAGGATATATTAGGATGCAA+AGG 0.511028 4.1:+26646648 MS.gene006665:CDS
GGTTATGGTGTCGGTAATGA+TGG 0.515702 4.1:+26646575 MS.gene006665:CDS
TAAGTTGAGTACTGGGAAGT+TGG 0.520530 4.1:+26646187 MS.gene006665:CDS
GTGTTCCATCAGCAGTGGAT+TGG 0.522985 4.1:+26645970 MS.gene006665:CDS
TTGGTTGGTTAAGAATTCAT+GGG 0.526242 4.1:+26646607 MS.gene006665:CDS
GACACTTACCACATTGACCT+TGG 0.526963 4.1:-26646025 None:intergenic
ATTGAAGCTTCCAACAATGC+TGG 0.532605 4.1:+26645807 MS.gene006665:CDS
CTTGTTGATTGTGACACAAA+AGG 0.533495 4.1:+26646230 MS.gene006665:CDS
AGGACTTTGTGGCATTGCAA+TGG 0.537544 4.1:+26646688 MS.gene006665:CDS
TCTGCTGTTGCGGCAACCGA+AGG 0.538022 4.1:+26646158 MS.gene006665:CDS
GTTCCGTCAACACCTTGATA+GGG 0.541629 4.1:-26646341 None:intergenic
AGTTTGGAACTTAAGCTGTA+GGG 0.544894 4.1:-26646721 None:intergenic
GAATCCATAAGTTGAGTACT+GGG 0.546014 4.1:+26646180 MS.gene006665:CDS
GTGCCTGCTCATTGTTGGCA+GGG 0.546537 4.1:-26646421 None:intergenic
TCCGTAGCTATTGATGCTAG+TGG 0.550787 4.1:+26646473 MS.gene006665:CDS
GAAGCTCAATACCCCTATCA+AGG 0.559311 4.1:+26646329 MS.gene006665:CDS
TGTCCCTGCCAACAATGAGC+AGG 0.564412 4.1:+26646418 MS.gene006665:CDS
CCGTAGCTATTGATGCTAGT+GGG 0.567886 4.1:+26646474 MS.gene006665:CDS
GTAATGATGGAACCAAGTAT+TGG 0.568388 4.1:+26646588 MS.gene006665:CDS
ATGTTGATTATTGGCAGCCA+TGG 0.568696 4.1:-26645598 None:intergenic
TGATGGAACCAAGTATTGGT+TGG 0.568906 4.1:+26646592 MS.gene006665:CDS
AATTCATGGGGAACTGATTG+GGG 0.577168 4.1:+26646620 MS.gene006665:CDS
AGGGGAGTCGATGCTGCTCA+AGG 0.577650 4.1:+26646668 MS.gene006665:CDS
TTTCTTTGAATATCCTGAGA+CGG 0.585323 4.1:-26645773 None:intergenic
AGATGCAATGAACTCCTCGT+TGG 0.591480 4.1:-26645877 None:intergenic
GGTGTAGACCAAGGTTGTGA+GGG 0.591635 4.1:+26646251 MS.gene006665:CDS
GTAGACCAAGGTTGTGAGGG+TGG 0.591695 4.1:+26646254 MS.gene006665:CDS
TAAGGCCACCCTCACAACCT+TGG 0.592405 4.1:-26646259 None:intergenic
AGGTGTAGACCAAGGTTGTG+AGG 0.594253 4.1:+26646250 MS.gene006665:CDS
CAATGAACTCCTCGTTGGTG+AGG 0.595329 4.1:-26645872 None:intergenic
CCCACTAGCATCAATAGCTA+CGG 0.596676 4.1:-26646474 None:intergenic
GGTAATGGTAACTGCATGGA+TGG 0.597553 4.1:-26646384 None:intergenic
ACCCGGTAATGGTAACTGCA+TGG 0.598149 4.1:-26646388 None:intergenic
TGCAAGTGTTCCATCAGCAG+TGG 0.603452 4.1:+26645965 MS.gene006665:CDS
TTCCGTCAACACCTTGATAG+GGG 0.605371 4.1:-26646340 None:intergenic
GGCAGGGACATCTTCATACC+CGG 0.606676 4.1:-26646405 None:intergenic
GCATCTAGAAACAAATTCAA+GGG 0.607899 4.1:+26645894 MS.gene006665:CDS
TGGTTCATGTGGAACTGAGT+TGG 0.612813 4.1:+26646532 MS.gene006665:CDS
GACCATGGTGTCACTGCTGT+AGG 0.612821 4.1:+26646554 MS.gene006665:CDS
TCCATGCAGTTACCATTACC+GGG 0.614442 4.1:+26646387 MS.gene006665:CDS
CATGCTTCTCATACATGGAG+TGG 0.622290 4.1:-26645695 None:intergenic
TGGTTGGTTAAGAATTCATG+GGG 0.622660 4.1:+26646608 MS.gene006665:CDS
GGTGTCTTTACTGGTTCATG+TGG 0.637602 4.1:+26646521 MS.gene006665:CDS
AACCTACAGCAGTGACACCA+TGG 0.649313 4.1:-26646556 None:intergenic
TGTATGAGAAGCATGAGCAA+TGG 0.663906 4.1:+26645703 MS.gene006665:CDS
GGATATATTAGGATGCAAAG+GGG 0.671421 4.1:+26646650 MS.gene006665:CDS
GTGAAGAACCAAGGTCAATG+TGG 0.671761 4.1:+26646017 MS.gene006665:CDS
GCAGGCACTGCAGAAAGCTG+TGG 0.675386 4.1:+26646436 MS.gene006665:CDS
ACTGCTGTAGGTTATGGTGT+CGG 0.678447 4.1:+26646566 MS.gene006665:CDS
TGTGGAACTGAGTTGGACCA+TGG 0.679648 4.1:+26646539 MS.gene006665:CDS
GACACAAAAGGTGTAGACCA+AGG 0.707755 4.1:+26646242 MS.gene006665:CDS
GTAACACCAGTGAAGAACCA+AGG 0.718576 4.1:+26646008 MS.gene006665:CDS
ATTTGCAGACCTCACCAACG+AGG 0.760076 4.1:+26645863 MS.gene006665:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACAAGTTATACAAATT+AGG + chr4.1:26645831-26645850 MS.gene006665:CDS 15.0%
!!! AATTTTAATTTACCTTTTGT+AGG + chr4.1:26646119-26646138 MS.gene006665:intron 15.0%
!!! TTTTATCACATAATATGTAA+TGG - chr4.1:26646071-26646090 None:intergenic 15.0%
!! AAATTCATCATTCAAAATCA+TGG + chr4.1:26646296-26646315 MS.gene006665:CDS 20.0%
! TAATCAACATAACATGTCAT+TGG + chr4.1:26645611-26645630 MS.gene006665:CDS 25.0%
!! TGACATGTTATGTTGATTAT+TGG - chr4.1:26645610-26645629 None:intergenic 25.0%
AAGGATATATTAGGATGCAA+AGG + chr4.1:26646648-26646667 MS.gene006665:CDS 30.0%
AGGATATATTAGGATGCAAA+GGG + chr4.1:26646649-26646668 MS.gene006665:CDS 30.0%
ATTCTTAACCAACCAATACT+TGG - chr4.1:26646603-26646622 None:intergenic 30.0%
CTATAAGGATTCTCAAGAAA+GGG + chr4.1:26645746-26645765 MS.gene006665:CDS 30.0%
GCATCTAGAAACAAATTCAA+GGG + chr4.1:26645894-26645913 MS.gene006665:CDS 30.0%
TCTATAAGGATTCTCAAGAA+AGG + chr4.1:26645745-26645764 MS.gene006665:CDS 30.0%
TGCATCTAGAAACAAATTCA+AGG + chr4.1:26645893-26645912 MS.gene006665:CDS 30.0%
TTTCTTTGAATATCCTGAGA+CGG - chr4.1:26645776-26645795 None:intergenic 30.0%
! ATTGGTTGGTTAAGAATTCA+TGG + chr4.1:26646606-26646625 MS.gene006665:CDS 30.0%
! TTGGTTGGTTAAGAATTCAT+GGG + chr4.1:26646607-26646626 MS.gene006665:CDS 30.0%
!! ATTTGAAAGCATCATCCATA+AGG - chr4.1:26646280-26646299 None:intergenic 30.0%
ACTCAACTTATGGATTCCTT+CGG - chr4.1:26646177-26646196 None:intergenic 35.0%
AGTAGTTGTCTTTGTGATTG+AGG - chr4.1:26645931-26645950 None:intergenic 35.0%
GACTTTCAGTTCTACAAAAG+TGG + chr4.1:26646500-26646519 MS.gene006665:CDS 35.0%
GGATATATTAGGATGCAAAG+GGG + chr4.1:26646650-26646669 MS.gene006665:CDS 35.0%
TACAAAAGTGGTGTCTTTAC+TGG + chr4.1:26646512-26646531 MS.gene006665:CDS 35.0%
TCACTATGGCAAAGTCTATA+AGG + chr4.1:26645731-26645750 MS.gene006665:CDS 35.0%
! CTTGTTGATTGTGACACAAA+AGG + chr4.1:26646230-26646249 MS.gene006665:CDS 35.0%
! GAATCCATAAGTTGAGTACT+GGG + chr4.1:26646180-26646199 MS.gene006665:CDS 35.0%
! GTAATGATGGAACCAAGTAT+TGG + chr4.1:26646588-26646607 MS.gene006665:CDS 35.0%
! TGGTTGGTTAAGAATTCATG+GGG + chr4.1:26646608-26646627 MS.gene006665:CDS 35.0%
!! ATTGGCATTGCTTTTATGCT+TGG + chr4.1:26645629-26645648 MS.gene006665:CDS 35.0%
!! TTGGCATTGCTTTTATGCTT+GGG + chr4.1:26645630-26645649 MS.gene006665:CDS 35.0%
AATTCATGGGGAACTGATTG+GGG + chr4.1:26646620-26646639 MS.gene006665:CDS 40.0%
ACTTCCCAGTACTCAACTTA+TGG - chr4.1:26646187-26646206 None:intergenic 40.0%
AGAATTCATGGGGAACTGAT+TGG + chr4.1:26646618-26646637 MS.gene006665:CDS 40.0%
ATCCATGCAGTTACCATTAC+CGG + chr4.1:26646386-26646405 MS.gene006665:CDS 40.0%
ATTGAAGCTTCCAACAATGC+TGG + chr4.1:26645807-26645826 MS.gene006665:CDS 40.0%
GAATTCATGGGGAACTGATT+GGG + chr4.1:26646619-26646638 MS.gene006665:CDS 40.0%
GCATCAATAGCTACGGAAAT+TGG - chr4.1:26646470-26646489 None:intergenic 40.0%
GGGGTGAAGAAGGATATATT+AGG + chr4.1:26646639-26646658 MS.gene006665:CDS 40.0%
TTGCTCATGCTTCTCATACA+TGG - chr4.1:26645703-26645722 None:intergenic 40.0%
! ACCTTTTGTAGGATGTTGCT+GGG + chr4.1:26646130-26646149 MS.gene006665:intron 40.0%
! GGAATCCATAAGTTGAGTAC+TGG + chr4.1:26646179-26646198 MS.gene006665:CDS 40.0%
! TACCTTTTGTAGGATGTTGC+TGG + chr4.1:26646129-26646148 MS.gene006665:intron 40.0%
! TGGGAAGTTGGTCTCTTTAT+CGG + chr4.1:26646199-26646218 MS.gene006665:CDS 40.0%
! TGTATGAGAAGCATGAGCAA+TGG + chr4.1:26645703-26645722 MS.gene006665:CDS 40.0%
!! CTTGTTATTGCCAGCATTGT+TGG - chr4.1:26645820-26645839 None:intergenic 40.0%
!! TAAGTTGAGTACTGGGAAGT+TGG + chr4.1:26646187-26646206 MS.gene006665:CDS 40.0%
!! TGATGGAACCAAGTATTGGT+TGG + chr4.1:26646592-26646611 MS.gene006665:CDS 40.0%
ACTGCTGTAGGTTATGGTGT+CGG + chr4.1:26646566-26646585 MS.gene006665:CDS 45.0%
AGATGCAATGAACTCCTCGT+TGG - chr4.1:26645880-26645899 None:intergenic 45.0%
AGGACTTTGTGGCATTGCAA+TGG + chr4.1:26646688-26646707 MS.gene006665:CDS 45.0%
AGTTCCGTCAACACCTTGAT+AGG - chr4.1:26646345-26646364 None:intergenic 45.0%
CATACATGGAGTGGTCATCT+TGG - chr4.1:26645689-26645708 None:intergenic 45.0%
CATGCTTCTCATACATGGAG+TGG - chr4.1:26645698-26645717 None:intergenic 45.0%
CCCACTAGCATCAATAGCTA+CGG - chr4.1:26646477-26646496 None:intergenic 45.0%
CCGTAGCTATTGATGCTAGT+GGG + chr4.1:26646474-26646493 MS.gene006665:CDS 45.0%
GAAGCTCAATACCCCTATCA+AGG + chr4.1:26646329-26646348 MS.gene006665:CDS 45.0%
GACACTTACCACATTGACCT+TGG - chr4.1:26646028-26646047 None:intergenic 45.0%
GACATCTTCATACCCGGTAA+TGG - chr4.1:26646402-26646421 None:intergenic 45.0%
GAGCAATGGATGAGTCACTA+TGG + chr4.1:26645717-26645736 MS.gene006665:CDS 45.0%
GTAACACCAGTGAAGAACCA+AGG + chr4.1:26646008-26646027 MS.gene006665:CDS 45.0%
GTGAAGAACCAAGGTCAATG+TGG + chr4.1:26646017-26646036 MS.gene006665:CDS 45.0%
GTTCCGTCAACACCTTGATA+GGG - chr4.1:26646344-26646363 None:intergenic 45.0%
TCCATGCAGTTACCATTACC+GGG + chr4.1:26646387-26646406 MS.gene006665:CDS 45.0%
TCCGTAGCTATTGATGCTAG+TGG + chr4.1:26646473-26646492 MS.gene006665:CDS 45.0%
TGGTTCATGTGGAACTGAGT+TGG + chr4.1:26646532-26646551 MS.gene006665:CDS 45.0%
TTCCGTCAACACCTTGATAG+GGG - chr4.1:26646343-26646362 None:intergenic 45.0%
TTTCTCCAATCCACTGCTGA+TGG - chr4.1:26645978-26645997 None:intergenic 45.0%
! CATTGACCTTGGTTCTTCAC+TGG - chr4.1:26646017-26646036 None:intergenic 45.0%
! GACACAAAAGGTGTAGACCA+AGG + chr4.1:26646242-26646261 MS.gene006665:CDS 45.0%
! GGTAATGGTAACTGCATGGA+TGG - chr4.1:26646387-26646406 None:intergenic 45.0%
!! GCAGTGGATTGGAGAAAGAA+AGG + chr4.1:26645981-26646000 MS.gene006665:CDS 45.0%
!! GGTGTCTTTACTGGTTCATG+TGG + chr4.1:26646521-26646540 MS.gene006665:CDS 45.0%
!! GGTTATGGTGTCGGTAATGA+TGG + chr4.1:26646575-26646594 MS.gene006665:CDS 45.0%
!! TACCCCTATCAAGGTGTTGA+CGG + chr4.1:26646338-26646357 MS.gene006665:CDS 45.0%
AACCTACAGCAGTGACACCA+TGG - chr4.1:26646559-26646578 None:intergenic 50.0%
AAGAAAGGGAGAACCGTCTC+AGG + chr4.1:26645760-26645779 MS.gene006665:CDS 50.0%
AGGTGTAGACCAAGGTTGTG+AGG + chr4.1:26646250-26646269 MS.gene006665:CDS 50.0%
ATTTGCAGACCTCACCAACG+AGG + chr4.1:26645863-26645882 MS.gene006665:CDS 50.0%
CAATGAACTCCTCGTTGGTG+AGG - chr4.1:26645875-26645894 None:intergenic 50.0%
GATGCTGCTCAAGGACTTTG+TGG + chr4.1:26646677-26646696 MS.gene006665:CDS 50.0%
GCCCAGCAACATCCTACAAA+AGG - chr4.1:26646134-26646153 None:intergenic 50.0%
GGAACTGATTGGGGTGAAGA+AGG + chr4.1:26646629-26646648 MS.gene006665:CDS 50.0%
GGTGTAGACCAAGGTTGTGA+GGG + chr4.1:26646251-26646270 MS.gene006665:CDS 50.0%
GTGTTCCATCAGCAGTGGAT+TGG + chr4.1:26645970-26645989 MS.gene006665:CDS 50.0%
TGCAAGTGTTCCATCAGCAG+TGG + chr4.1:26645965-26645984 MS.gene006665:CDS 50.0%
! ACCCGGTAATGGTAACTGCA+TGG - chr4.1:26646391-26646410 None:intergenic 50.0%
! CTGGGCATTTTCTGCTGTTG+CGG + chr4.1:26646148-26646167 MS.gene006665:CDS 50.0%
! TGTGGAACTGAGTTGGACCA+TGG + chr4.1:26646539-26646558 MS.gene006665:CDS 50.0%
!! GGTGTCACTGCTGTAGGTTA+TGG + chr4.1:26646560-26646579 MS.gene006665:CDS 50.0%
AGTGCCTGCTCATTGTTGGC+AGG - chr4.1:26646425-26646444 None:intergenic 55.0%
CTGCAGTGCCTGCTCATTGT+TGG - chr4.1:26646429-26646448 None:intergenic 55.0%
GGCAGGGACATCTTCATACC+CGG - chr4.1:26646408-26646427 None:intergenic 55.0%
GTAGACCAAGGTTGTGAGGG+TGG + chr4.1:26646254-26646273 MS.gene006665:CDS 55.0%
GTGCCTGCTCATTGTTGGCA+GGG - chr4.1:26646424-26646443 None:intergenic 55.0%
TAAGGCCACCCTCACAACCT+TGG - chr4.1:26646262-26646281 None:intergenic 55.0%
TGTCCCTGCCAACAATGAGC+AGG + chr4.1:26646418-26646437 MS.gene006665:CDS 55.0%
! AGGTTGTGAGGGTGGCCTTA+TGG + chr4.1:26646262-26646281 MS.gene006665:CDS 55.0%
!! GACCATGGTGTCACTGCTGT+AGG + chr4.1:26646554-26646573 MS.gene006665:CDS 55.0%
AGGGGAGTCGATGCTGCTCA+AGG + chr4.1:26646668-26646687 MS.gene006665:CDS 60.0%
TCTGCTGTTGCGGCAACCGA+AGG + chr4.1:26646158-26646177 MS.gene006665:CDS 60.0%
! GCAGGCACTGCAGAAAGCTG+TGG + chr4.1:26646436-26646455 MS.gene006665:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 26645600 26646733 26645600 ID=MS.gene006665
chr4.1 mRNA 26645600 26646733 26645600 ID=MS.gene006665.t1;Parent=MS.gene006665
chr4.1 exon 26645600 26646038 26645600 ID=MS.gene006665.t1.exon1;Parent=MS.gene006665.t1
chr4.1 CDS 26645600 26646038 26645600 ID=cds.MS.gene006665.t1;Parent=MS.gene006665.t1
chr4.1 exon 26646141 26646733 26646141 ID=MS.gene006665.t1.exon2;Parent=MS.gene006665.t1
chr4.1 CDS 26646141 26646733 26646141 ID=cds.MS.gene006665.t1;Parent=MS.gene006665.t1
Gene Sequence

>MS.gene006665

ATGGCTGCCAATAATCAACATAACATGTCATTGGCATTGCTTTTATGCTTGGGATTGTTTGCTATTCAAGTCACTTCTCGTACTCTCCAAGATGACCACTCCATGTATGAGAAGCATGAGCAATGGATGAGTCACTATGGCAAAGTCTATAAGGATTCTCAAGAAAGGGAGAACCGTCTCAGGATATTCAAAGAAAATGTGAATTACATTGAAGCTTCCAACAATGCTGGCAATAACAAGTTATACAAATTAGGCATTAATCAATTTGCAGACCTCACCAACGAGGAGTTCATTGCATCTAGAAACAAATTCAAGGGTCATATGTGCTCCTCAATCACAAAGACAACTACTTTTATGTATGAAAATGCAAGTGTTCCATCAGCAGTGGATTGGAGAAAGAAAGGAGCAGTAACACCAGTGAAGAACCAAGGTCAATGTGGTAAGTGTCTATCTAATATGTAATGCATTCCATTACATATTATGTGATAAAAAAAAATGAATTGTCATTAAACTCTAGTTAATTTTAATTTACCTTTTGTAGGATGTTGCTGGGCATTTTCTGCTGTTGCGGCAACCGAAGGAATCCATAAGTTGAGTACTGGGAAGTTGGTCTCTTTATCGGAACAAGAACTTGTTGATTGTGACACAAAAGGTGTAGACCAAGGTTGTGAGGGTGGCCTTATGGATGATGCTTTCAAATTCATCATTCAAAATCATGGACTCAACACTGAAGCTCAATACCCCTATCAAGGTGTTGACGGAACTTGCAGTGCAAACAAAGCATCCATCCATGCAGTTACCATTACCGGGTATGAAGATGTCCCTGCCAACAATGAGCAGGCACTGCAGAAAGCTGTGGCAAATCAACCAATTTCCGTAGCTATTGATGCTAGTGGGTCTGACTTTCAGTTCTACAAAAGTGGTGTCTTTACTGGTTCATGTGGAACTGAGTTGGACCATGGTGTCACTGCTGTAGGTTATGGTGTCGGTAATGATGGAACCAAGTATTGGTTGGTTAAGAATTCATGGGGAACTGATTGGGGTGAAGAAGGATATATTAGGATGCAAAGGGGAGTCGATGCTGCTCAAGGACTTTGTGGCATTGCAATGGAAGCATCTTACCCTACAGCTTAA
Protein sequence

>MS.gene006665.t1

MAANNQHNMSLALLLCLGLFAIQVTSRTLQDDHSMYEKHEQWMSHYGKVYKDSQERENRLRIFKENVNYIEASNNAGNNKLYKLGINQFADLTNEEFIASRNKFKGHMCSSITKTTTFMYENASVPSAVDWRKKGAVTPVKNQGQCGCCWAFSAVAATEGIHKLSTGKLVSLSEQELVDCDTKGVDQGCEGGLMDDAFKFIIQNHGLNTEAQYPYQGVDGTCSANKASIHAVTITGYEDVPANNEQALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGVGNDGTKYWLVKNSWGTDWGEEGYIRMQRGVDAAQGLCGIAMEASYPTA