AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059966

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059966.t1 MTR_4g104370 96.111 360 14 0 1 360 1 360 0.0 730
MS.gene059966.t1 MTR_3g075390 74.033 362 92 2 1 360 1 362 0.0 576
MS.gene059966.t1 MTR_4g079770 54.885 348 146 9 1 342 1 343 9.64e-128 370
MS.gene059966.t1 MTR_4g107930 53.890 347 151 7 1 342 1 343 8.63e-127 368
MS.gene059966.t1 MTR_5g022560 54.519 343 145 9 5 342 6 342 3.09e-126 366
MS.gene059966.t1 MTR_4g079470 57.862 318 124 8 30 342 31 343 5.28e-126 366
MS.gene059966.t1 MTR_4g047610 59.105 313 116 8 38 342 33 341 2.08e-125 364
MS.gene059966.t1 MTR_4g079800 57.233 318 126 8 30 342 31 343 9.18e-124 360
MS.gene059966.t1 MTR_3g116080 53.736 348 149 7 5 345 7 349 1.19e-123 360
MS.gene059966.t1 MTR_4g080700 58.013 312 119 8 35 342 35 338 4.58e-122 355
MS.gene059966.t1 MTR_4g080730 57.098 317 124 8 30 342 30 338 6.14e-122 355
MS.gene059966.t1 MTR_1g107550 55.015 329 138 7 23 345 33 357 3.81e-120 355
MS.gene059966.t1 MTR_2g075830 54.360 344 141 9 5 342 6 339 3.28e-119 348
MS.gene059966.t1 MTR_1g018840 50.704 355 162 8 1 344 10 362 4.20e-118 350
MS.gene059966.t1 MTR_1g018840 50.704 355 162 8 1 344 10 362 9.16e-118 349
MS.gene059966.t1 MTR_4g094918 49.713 348 162 7 1 344 4 342 1.85e-117 345
MS.gene059966.t1 MTR_4g079440 55.375 307 126 6 40 343 46 344 1.89e-116 342
MS.gene059966.t1 MTR_3g102220 51.149 348 158 9 7 345 19 363 2.08e-114 341
MS.gene059966.t1 MTR_4g080360 49.682 314 153 4 33 343 30 341 1.08e-109 324
MS.gene059966.t1 MTR_2g090890 51.948 308 136 6 40 344 39 337 1.95e-108 321
MS.gene059966.t1 MTR_2g083930 48.378 339 161 7 11 343 13 343 7.09e-107 317
MS.gene059966.t1 MTR_7g078600 50.464 323 140 10 26 343 30 337 8.61e-104 309
MS.gene059966.t1 MTR_7g079230 47.904 334 157 8 13 342 14 334 1.13e-97 293
MS.gene059966.t1 MTR_3g112430 48.875 311 155 4 39 346 35 344 1.47e-96 294
MS.gene059966.t1 MTR_2g015850 46.128 297 155 4 48 343 71 363 2.63e-84 260
MS.gene059966.t1 MTR_2g016270 42.286 350 167 12 8 345 16 342 1.67e-83 261
MS.gene059966.t1 MTR_2g016180 42.571 350 181 12 8 345 16 357 1.95e-83 262
MS.gene059966.t1 MTR_4g065077 40.503 358 180 15 6 344 14 357 6.06e-71 226
MS.gene059966.t1 MTR_2g067900 41.317 334 175 12 22 343 2 326 5.66e-69 219
MS.gene059966.t1 MTR_2g015920 37.463 339 188 12 30 355 43 370 1.48e-66 215
MS.gene059966.t1 MTR_8g086470 39.660 353 174 14 6 344 30 357 1.79e-65 211
MS.gene059966.t1 MTR_4g125300 42.904 303 144 13 52 343 64 348 4.63e-65 210
MS.gene059966.t1 MTR_2g067560 40.533 338 169 15 40 356 16 342 7.78e-65 221
MS.gene059966.t1 MTR_2g015930 37.879 330 178 14 30 344 42 359 2.12e-61 201
MS.gene059966.t1 MTR_1g023210 38.742 302 150 12 54 335 66 352 2.42e-58 193
MS.gene059966.t1 MTR_2g064475 35.965 342 170 13 40 341 16 348 6.40e-57 189
MS.gene059966.t1 MTR_4g071060 37.377 305 159 10 49 335 58 348 2.95e-55 185
MS.gene059966.t1 MTR_2g066980 35.202 321 153 11 40 342 34 317 8.15e-55 182
MS.gene059966.t1 MTR_7g078630 40.264 303 130 12 19 306 687 953 1.36e-54 193
MS.gene059966.t1 MTR_1g023810 41.150 226 124 6 120 341 41 261 2.00e-54 180
MS.gene059966.t1 MTR_1g094135 33.728 338 194 10 15 335 34 358 6.46e-54 182
MS.gene059966.t1 MTR_7g078580 65.657 99 33 1 245 343 4 101 1.05e-42 145
MS.gene059966.t1 MTR_4g072790 36.644 292 143 13 49 335 58 312 1.46e-40 145
MS.gene059966.t1 MTR_7g079130 67.033 91 30 0 253 343 7 97 1.49e-39 135
MS.gene059966.t1 MTR_4g072340 34.237 295 150 11 49 335 58 316 3.80e-38 139
MS.gene059966.t1 MTR_4g073660 34.797 296 147 13 49 335 57 315 6.46e-38 139
MS.gene059966.t1 MTR_1g025450 40.556 180 82 7 124 298 51 210 1.88e-34 133
MS.gene059966.t1 MTR_2g015840 50.450 111 54 1 232 342 161 270 4.85e-33 124
MS.gene059966.t1 MTR_2g015840 34.615 156 95 5 11 163 5 156 4.15e-20 89.4
MS.gene059966.t1 MTR_2g016380 43.089 123 63 4 227 345 18 137 2.77e-27 108
MS.gene059966.t1 MTR_2g047960 65.385 78 26 1 138 214 8 85 1.46e-25 99.4
MS.gene059966.t1 MTR_3g029560 31.604 212 115 6 139 343 49 237 1.91e-23 97.8
MS.gene059966.t1 MTR_7g111060 26.906 223 137 7 134 335 113 330 5.51e-22 95.9
MS.gene059966.t1 MTR_7g111050 29.630 243 143 8 126 345 102 339 7.20e-22 95.5
MS.gene059966.t1 MTR_7g079260 56.944 72 15 1 269 340 7 62 2.43e-21 88.2
MS.gene059966.t1 MTR_7g078310 39.041 146 74 5 75 216 110 244 1.09e-19 87.8
MS.gene059966.t1 MTR_7g111050 28.713 202 122 6 126 306 97 297 4.33e-14 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059966.t1 AT5G50260 69.252 361 108 2 3 360 1 361 0.0 533
MS.gene059966.t1 AT3G48340 65.193 362 121 4 3 360 1 361 5.73e-173 486
MS.gene059966.t1 AT3G48350 62.778 360 132 2 3 360 1 360 5.79e-169 476
MS.gene059966.t1 AT3G48350 62.094 277 103 2 3 277 1 277 1.54e-123 358
MS.gene059966.t1 AT1G20850 53.416 322 145 4 25 344 36 354 1.44e-119 350
MS.gene059966.t1 AT5G45890 49.575 353 151 9 3 342 6 344 1.06e-118 347
MS.gene059966.t1 AT4G35350 50.432 347 157 6 6 344 14 353 1.18e-118 347
MS.gene059966.t1 AT3G19390 50.142 353 163 7 1 349 8 351 8.88e-117 346
MS.gene059966.t1 AT5G43060 51.934 362 150 8 1 345 1 355 1.12e-116 346
MS.gene059966.t1 AT1G47128 53.312 317 139 4 32 344 42 353 2.47e-113 338
MS.gene059966.t1 AT1G06260 48.837 344 160 7 7 344 10 343 8.63e-112 330
MS.gene059966.t1 AT4G36880 48.943 331 157 7 32 352 41 369 1.13e-109 325
MS.gene059966.t1 AT3G19400 47.536 345 158 10 18 356 26 353 4.43e-103 308
MS.gene059966.t1 AT2G27420 49.533 321 151 6 33 343 28 347 1.91e-102 306
MS.gene059966.t1 AT4G11310 44.693 358 176 11 4 346 5 355 1.51e-99 299
MS.gene059966.t1 AT3G49340 47.785 316 157 6 33 343 28 340 1.78e-99 298
MS.gene059966.t1 AT4G11320 47.923 313 153 7 39 346 55 362 5.64e-99 298
MS.gene059966.t1 AT4G11320 47.923 313 153 7 39 346 55 362 5.64e-99 298
MS.gene059966.t1 AT4G23520 46.203 316 159 7 39 349 46 355 8.34e-98 294
MS.gene059966.t1 AT2G34080 44.857 350 171 7 6 343 5 344 1.80e-97 293
MS.gene059966.t1 AT1G09850 48.065 310 153 6 38 344 30 334 5.51e-94 287
MS.gene059966.t1 AT1G29090 43.678 348 188 6 2 343 9 354 9.16e-92 279
MS.gene059966.t1 AT1G29080 44.720 322 165 9 30 343 29 345 1.59e-87 268
MS.gene059966.t1 AT4G35350 48.399 281 128 6 7 277 13 286 4.34e-85 259
MS.gene059966.t1 AT3G43960 42.442 344 178 11 13 347 18 350 1.73e-79 248
MS.gene059966.t1 AT1G29110 38.551 345 186 10 7 343 7 333 1.88e-73 231
MS.gene059966.t1 AT3G19400 46.360 261 126 8 18 272 26 278 2.67e-69 219
MS.gene059966.t1 AT5G60360 41.667 300 154 11 51 343 71 356 3.16e-64 208
MS.gene059966.t1 AT3G45310 41.333 300 155 11 51 343 71 356 1.68e-62 204
MS.gene059966.t1 AT5G60360 41.333 300 154 12 51 343 71 355 1.06e-61 202
MS.gene059966.t1 AT3G45310 41.000 300 155 12 51 343 71 355 6.72e-60 197
MS.gene059966.t1 AT5G60360 41.786 280 146 10 51 323 71 340 1.58e-59 196
MS.gene059966.t1 AT4G39090 38.835 309 160 9 44 335 56 352 1.16e-58 194
MS.gene059966.t1 AT2G21430 35.143 350 194 9 6 335 13 349 2.27e-58 193
MS.gene059966.t1 AT4G16190 36.700 297 163 8 55 335 71 358 1.62e-52 178
MS.gene059966.t1 AT3G54940 36.120 299 164 9 54 335 66 354 3.48e-50 172
MS.gene059966.t1 AT3G54940 38.500 200 110 5 54 244 66 261 1.41e-35 131
MS.gene059966.t1 AT1G02305 28.763 299 181 10 85 360 68 357 3.72e-26 107
MS.gene059966.t1 AT2G22160 51.042 96 35 2 60 151 15 102 1.18e-21 89.0
MS.gene059966.t1 AT4G01610 26.241 282 167 11 85 338 65 333 1.46e-21 94.7
MS.gene059966.t1 AT1G02300 29.114 237 137 8 147 360 146 374 2.11e-21 94.7
MS.gene059966.t1 AT4G01610 25.532 282 169 11 85 338 65 333 1.19e-19 89.4

Find 83 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGGCTTATCTAATTTAT+TGG 0.217778 8.1:-20068938 None:intergenic
ACATGACAAATTATGAATTT+AGG 0.245011 8.1:+20068986 MS.gene059966:CDS
TTATTCAACTTAAGCTTATA+AGG 0.250519 8.1:-20068955 None:intergenic
AGCTAAGAGCTTTGATTTCC+AGG 0.263284 8.1:+20068777 MS.gene059966:CDS
AGGATGCAACGGTGGGTTAA+TGG 0.279883 8.1:+20069553 MS.gene059966:CDS
TAACTTGTATAGGTAGTTGC+TGG 0.293976 8.1:+20069423 MS.gene059966:intron
GCATCAATAGCTACAGAAAT+AGG 0.294697 8.1:-20069876 None:intergenic
ATCATTTAAAGCTCATCTTT+TGG 0.331162 8.1:-20070273 None:intergenic
CTCAAGATGGAACAAAATAT+TGG 0.333579 8.1:+20070097 MS.gene059966:CDS
GAGCATCTATGCTGATTCTA+AGG 0.335794 8.1:+20069008 MS.gene059966:CDS
GGGAAGTGAATGGGGAGAAC+AGG 0.351413 8.1:+20070137 MS.gene059966:CDS
CAACAATGATGCTTCATTGT+TGG 0.354576 8.1:-20069835 None:intergenic
TAATTACAGGGAGTATTTAC+TGG 0.363002 8.1:+20070021 MS.gene059966:intron
TCGATTGATTGGAGGAAGAT+AGG 0.372632 8.1:+20069111 MS.gene059966:CDS
TTCGAGTTCATCAAGCAAAA+TGG 0.391365 8.1:+20069584 MS.gene059966:CDS
TCAACAATTGTAGCTGTTGA+AGG 0.396428 8.1:+20069452 MS.gene059966:CDS
ATGTTCCTTCTTCGATTGAT+TGG 0.402034 8.1:+20069100 MS.gene059966:CDS
ATTGGACAGTGAAGAACTCT+TGG 0.427329 8.1:+20070115 MS.gene059966:CDS
GAACTCTTGGGGAAGTGAAT+GGG 0.438030 8.1:+20070128 MS.gene059966:CDS
TATCTAATTTATTGGTATTG+TGG 0.443235 8.1:-20068930 None:intergenic
AAGCAAGCAGTGTCAATTGA+TGG 0.446024 8.1:+20069795 MS.gene059966:CDS
GGGGTAGCAATTGTGGGGTA+TGG 0.459702 8.1:+20070069 MS.gene059966:CDS
AATGATGCTTCATTGTTGGC+AGG 0.460490 8.1:-20069831 None:intergenic
TAAAATCAAAGCTAGGGATA+AGG 0.466804 8.1:-20068749 None:intergenic
AGGTGCTGTCACCGGGGTAA+AGG 0.468205 8.1:+20069131 MS.gene059966:CDS
GGAAGATAGGTGCTGTCACC+GGG 0.470663 8.1:+20069124 MS.gene059966:CDS
AGCATCAGAGAAAAGTTTGT+GGG 0.476908 8.1:+20068810 MS.gene059966:CDS
GAATCATGGGGTAGCAATTG+TGG 0.479525 8.1:+20070062 MS.gene059966:CDS
TTGGACAGTGAAGAACTCTT+GGG 0.479993 8.1:+20070116 MS.gene059966:CDS
AATCATGGGGTAGCAATTGT+GGG 0.482862 8.1:+20070063 MS.gene059966:CDS
AGAACTCTTGGGGAAGTGAA+TGG 0.483270 8.1:+20070127 MS.gene059966:CDS
TTATCCTTATGCTGCAAAAG+AGG 0.485453 8.1:+20069625 MS.gene059966:CDS
TGTGGGATTTGTACGAGAGA+TGG 0.486184 8.1:+20068827 MS.gene059966:CDS
AACTTGTATAGGTAGTTGCT+GGG 0.493124 8.1:+20069424 MS.gene059966:intron
CTATTCCTAAAATCAAAGCT+AGG 0.494841 8.1:-20068756 None:intergenic
AGCTACAGAAATAGGTTGGT+TGG 0.500901 8.1:-20069868 None:intergenic
GGAAGTGAATGGGGAGAACA+GGG 0.501956 8.1:+20070138 MS.gene059966:CDS
AGGTCTATGTGGCATAGCTA+TGG 0.507498 8.1:+20070197 MS.gene059966:CDS
TTCCTTCTTCGATTGATTGG+AGG 0.508300 8.1:+20069103 MS.gene059966:CDS
TATCCTTATGCTGCAAAAGA+GGG 0.511600 8.1:+20069626 MS.gene059966:CDS
TCTGTAGCTATTGATGCTGG+TGG 0.512891 8.1:+20069882 MS.gene059966:CDS
ATTTCTGTAGCTATTGATGC+TGG 0.514909 8.1:+20069879 MS.gene059966:CDS
TGCGACACTGCAGTGAATCA+AGG 0.524570 8.1:+20069533 MS.gene059966:CDS
AGGAAGATAGGTGCTGTCAC+CGG 0.527243 8.1:+20069123 MS.gene059966:CDS
AACAAGTAAGCTTACCGCAT+TGG 0.533561 8.1:-20069161 None:intergenic
TGTGGTACTGAGCTGAATCA+TGG 0.537115 8.1:+20070048 MS.gene059966:CDS
TTCCTCCAATCAATCGAAGA+AGG 0.537545 8.1:-20069105 None:intergenic
TATTCCTAAAATCAAAGCTA+GGG 0.538115 8.1:-20068755 None:intergenic
GGGAACCTGTAATACACAAA+AGG 0.540008 8.1:+20069646 MS.gene059966:CDS
CTGATGCTAAGTCATTCTCC+TGG 0.541617 8.1:-20068795 None:intergenic
GTGGTACTGAGCTGAATCAT+GGG 0.550981 8.1:+20070049 MS.gene059966:CDS
GGGTTACATTAGAATGCAAA+GGG 0.552847 8.1:+20070158 MS.gene059966:CDS
GGGTATGGAGCAACTCAAGA+TGG 0.553677 8.1:+20070084 MS.gene059966:CDS
CACAAGTTCTTGCTCAGACA+AGG 0.554554 8.1:-20069507 None:intergenic
GTCACCGGGGTAAAGGATCA+AGG 0.557892 8.1:+20069138 MS.gene059966:CDS
TGTTGGCAGGGACATTCTCA+TGG 0.563794 8.1:-20069818 None:intergenic
CTTGTCTGAGCAAGAACTTG+TGG 0.566708 8.1:+20069508 MS.gene059966:CDS
AACTCTTGGGGAAGTGAATG+GGG 0.569712 8.1:+20070129 MS.gene059966:CDS
ATGATGCTTCATTGTTGGCA+GGG 0.574434 8.1:-20069830 None:intergenic
CAATAGCTACAGAAATAGGT+TGG 0.582579 8.1:-20069872 None:intergenic
TAGCATCAGAGAAAAGTTTG+TGG 0.583867 8.1:+20068809 MS.gene059966:CDS
CACTGTCATGTGATAATCCA+CGG 0.585374 8.1:-20069050 None:intergenic
AGGGTTACATTAGAATGCAA+AGG 0.596326 8.1:+20070157 MS.gene059966:CDS
TCTATGTGGCATAGCTATGG+AGG 0.601740 8.1:+20070200 MS.gene059966:CDS
GTGAATCAAGGATGCAACGG+TGG 0.601861 8.1:+20069545 MS.gene059966:CDS
TCTCACAAGCAAGGTCTATG+TGG 0.620594 8.1:+20070186 MS.gene059966:CDS
ACCGTTCATGTATGAGAATG+TGG 0.632552 8.1:+20069074 MS.gene059966:CDS
TGAATCAAGGATGCAACGGT+GGG 0.634962 8.1:+20069546 MS.gene059966:CDS
TTGGCCTTGATCCTTTACCC+CGG 0.635267 8.1:-20069142 None:intergenic
TCCACATTCTCATACATGAA+CGG 0.636776 8.1:-20069075 None:intergenic
AATCATCATAGAATGTTCCG+TGG 0.642215 8.1:+20069033 MS.gene059966:CDS
GCAGTGAATCAAGGATGCAA+CGG 0.651301 8.1:+20069542 MS.gene059966:CDS
TGGACAGTGAAGAACTCTTG+GGG 0.663115 8.1:+20070117 MS.gene059966:CDS
ATTGTGGACATGCATAACAT+TGG 0.668124 8.1:-20068914 None:intergenic
AGGGACATATCTCACAAGCA+AGG 0.668337 8.1:+20070177 MS.gene059966:CDS
ATCATGGGGTAGCAATTGTG+GGG 0.693047 8.1:+20070064 MS.gene059966:CDS
CGTGGATTATCACATGACAG+TGG 0.715484 8.1:+20069051 MS.gene059966:CDS
CAAGACTTCGAGTAACTGTG+TGG 0.728098 8.1:-20068858 None:intergenic
GGAGTATTTACTGGACACTG+TGG 0.740210 8.1:+20070030 MS.gene059966:CDS
TGGTACTGAGCTGAATCATG+GGG 0.741700 8.1:+20070050 MS.gene059966:CDS
GAAGATAGGTGCTGTCACCG+GGG 0.757764 8.1:+20069125 MS.gene059966:CDS
GTTCATGTATGAGAATGTGG+AGG 0.769545 8.1:+20069077 MS.gene059966:CDS
GTAAAGGATCAAGGCCAATG+CGG 0.813840 8.1:+20069147 MS.gene059966:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATATATAAATGTGTAA+GGG - chr8.1:20069954-20069973 None:intergenic 10.0%
!! AATTACAAAAATATAATTAC+AGG + chr8.1:20070008-20070027 MS.gene059966:intron 10.0%
!! ATTACAAAAATATAATTACA+GGG + chr8.1:20070009-20070028 MS.gene059966:intron 10.0%
!!! ATATTTTTGTAATTAAAAGT+GGG - chr8.1:20070002-20070021 None:intergenic 10.0%
!!! TATATTTTTGTAATTAAAAG+TGG - chr8.1:20070003-20070022 None:intergenic 10.0%
!! AAAATGTGTTAATACTTAAT+AGG - chr8.1:20069757-20069776 None:intergenic 15.0%
!! CAAAAATATATAAATGTGTA+AGG - chr8.1:20069955-20069974 None:intergenic 15.0%
!! ACATGACAAATTATGAATTT+AGG + chr8.1:20068986-20069005 MS.gene059966:CDS 20.0%
!! ATAAGGCTTATCTAATTTAT+TGG - chr8.1:20068941-20068960 None:intergenic 20.0%
!! TATCTAATTTATTGGTATTG+TGG - chr8.1:20068933-20068952 None:intergenic 20.0%
!! TTATTCAACTTAAGCTTATA+AGG - chr8.1:20068958-20068977 None:intergenic 20.0%
! ATAATCAAATACAGACTAAG+TGG - chr8.1:20069300-20069319 None:intergenic 25.0%
! TATTCCTAAAATCAAAGCTA+GGG - chr8.1:20068758-20068777 None:intergenic 25.0%
! TCTGTATTTGATTATACGTT+TGG + chr8.1:20069305-20069324 MS.gene059966:intron 25.0%
!! TTTTCAGATGACTTTTTGAT+TGG - chr8.1:20070234-20070253 None:intergenic 25.0%
!!! GAATTGTTTCTAACTTGTAT+AGG + chr8.1:20069413-20069432 MS.gene059966:intron 25.0%
CTATTCCTAAAATCAAAGCT+AGG - chr8.1:20068759-20068778 None:intergenic 30.0%
CTCAAGATGGAACAAAATAT+TGG + chr8.1:20070097-20070116 MS.gene059966:CDS 30.0%
TAAAATCAAAGCTAGGGATA+AGG - chr8.1:20068752-20068771 None:intergenic 30.0%
TGTAATTAAAAGTGGGATCA+AGG - chr8.1:20069995-20070014 None:intergenic 30.0%
TTCAAGCGATTTAACTAACA+TGG - chr8.1:20069254-20069273 None:intergenic 30.0%
! AACACATTTTCACTTGATGT+AGG + chr8.1:20069767-20069786 MS.gene059966:intron 30.0%
! GTTTTACCTCTGAATAAAAC+TGG - chr8.1:20069917-20069936 None:intergenic 30.0%
! TTGGAAGTGAACTTTTTGTT+GGG - chr8.1:20070257-20070276 None:intergenic 30.0%
! TTTGGAAGTGAACTTTTTGT+TGG - chr8.1:20070258-20070277 None:intergenic 30.0%
!! AGATGACTTTTTGATTGGAT+AGG - chr8.1:20070229-20070248 None:intergenic 30.0%
!! CTAGTTTTTGTGTCTTGATT+TGG - chr8.1:20069484-20069503 None:intergenic 30.0%
!! GTTCTGAAGTAGAATTGATT+TGG + chr8.1:20069384-20069403 MS.gene059966:intron 30.0%
!! TAATTACAGGGAGTATTTAC+TGG + chr8.1:20070021-20070040 MS.gene059966:intron 30.0%
!!! TGATTTCCAGTTTTATTCAG+AGG + chr8.1:20069908-20069927 MS.gene059966:CDS 30.0%
!!! TTATCCCTAGCTTTGATTTT+AGG + chr8.1:20068751-20068770 MS.gene059966:CDS 30.0%
AACTTGTATAGGTAGTTGCT+GGG + chr8.1:20069424-20069443 MS.gene059966:intron 35.0%
AATCATCATAGAATGTTCCG+TGG + chr8.1:20069033-20069052 MS.gene059966:CDS 35.0%
AGCATCAGAGAAAAGTTTGT+GGG + chr8.1:20068810-20068829 MS.gene059966:CDS 35.0%
AGGGTTACATTAGAATGCAA+AGG + chr8.1:20070157-20070176 MS.gene059966:CDS 35.0%
ATGTTCCTTCTTCGATTGAT+TGG + chr8.1:20069100-20069119 MS.gene059966:CDS 35.0%
ATTGTGGACATGCATAACAT+TGG - chr8.1:20068917-20068936 None:intergenic 35.0%
ATTTCTGTAGCTATTGATGC+TGG + chr8.1:20069879-20069898 MS.gene059966:CDS 35.0%
CAACAATGATGCTTCATTGT+TGG - chr8.1:20069838-20069857 None:intergenic 35.0%
CAATAGCTACAGAAATAGGT+TGG - chr8.1:20069875-20069894 None:intergenic 35.0%
GCATCAATAGCTACAGAAAT+AGG - chr8.1:20069879-20069898 None:intergenic 35.0%
GGGTTACATTAGAATGCAAA+GGG + chr8.1:20070158-20070177 MS.gene059966:CDS 35.0%
GTTTGGATCCAAAATTGATC+TGG + chr8.1:20069322-20069341 MS.gene059966:intron 35.0%
TAGCATCAGAGAAAAGTTTG+TGG + chr8.1:20068809-20068828 MS.gene059966:CDS 35.0%
TATCCTTATGCTGCAAAAGA+GGG + chr8.1:20069626-20069645 MS.gene059966:CDS 35.0%
TCAACAATTGTAGCTGTTGA+AGG + chr8.1:20069452-20069471 MS.gene059966:CDS 35.0%
TCCACATTCTCATACATGAA+CGG - chr8.1:20069078-20069097 None:intergenic 35.0%
TTATCCTTATGCTGCAAAAG+AGG + chr8.1:20069625-20069644 MS.gene059966:CDS 35.0%
! TAACTTGTATAGGTAGTTGC+TGG + chr8.1:20069423-20069442 MS.gene059966:intron 35.0%
! TTCGAGTTCATCAAGCAAAA+TGG + chr8.1:20069584-20069603 MS.gene059966:CDS 35.0%
AACAAGTAAGCTTACCGCAT+TGG - chr8.1:20069164-20069183 None:intergenic 40.0%
AAGCAAGCAGTGTCAATTGA+TGG + chr8.1:20069795-20069814 MS.gene059966:CDS 40.0%
AATCATGGGGTAGCAATTGT+GGG + chr8.1:20070063-20070082 MS.gene059966:CDS 40.0%
AATGATGCTTCATTGTTGGC+AGG - chr8.1:20069834-20069853 None:intergenic 40.0%
ACCGTTCATGTATGAGAATG+TGG + chr8.1:20069074-20069093 MS.gene059966:CDS 40.0%
ATGATGCTTCATTGTTGGCA+GGG - chr8.1:20069833-20069852 None:intergenic 40.0%
ATTGGACAGTGAAGAACTCT+TGG + chr8.1:20070115-20070134 MS.gene059966:CDS 40.0%
CACTGTCATGTGATAATCCA+CGG - chr8.1:20069053-20069072 None:intergenic 40.0%
CCAAAATTGATCTGGAGAAC+TGG + chr8.1:20069330-20069349 MS.gene059966:intron 40.0%
GGGAACCTGTAATACACAAA+AGG + chr8.1:20069646-20069665 MS.gene059966:CDS 40.0%
GTTCATGTATGAGAATGTGG+AGG + chr8.1:20069077-20069096 MS.gene059966:CDS 40.0%
TCGATTGATTGGAGGAAGAT+AGG + chr8.1:20069111-20069130 MS.gene059966:CDS 40.0%
TTCCTCCAATCAATCGAAGA+AGG - chr8.1:20069108-20069127 None:intergenic 40.0%
TTCCTTCTTCGATTGATTGG+AGG + chr8.1:20069103-20069122 MS.gene059966:CDS 40.0%
TTGGACAGTGAAGAACTCTT+GGG + chr8.1:20070116-20070135 MS.gene059966:CDS 40.0%
! AGCTAAGAGCTTTGATTTCC+AGG + chr8.1:20068777-20068796 MS.gene059966:CDS 40.0%
! CCAGTTCTCCAGATCAATTT+TGG - chr8.1:20069333-20069352 None:intergenic 40.0%
! GTGCACCTTTTGTGTATTAC+AGG - chr8.1:20069654-20069673 None:intergenic 40.0%
!! AGCTACAGAAATAGGTTGGT+TGG - chr8.1:20069871-20069890 None:intergenic 40.0%
!! GAGCATCTATGCTGATTCTA+AGG + chr8.1:20069008-20069027 MS.gene059966:CDS 40.0%
AACTCTTGGGGAAGTGAATG+GGG + chr8.1:20070129-20070148 MS.gene059966:CDS 45.0%
AGAACTCTTGGGGAAGTGAA+TGG + chr8.1:20070127-20070146 MS.gene059966:CDS 45.0%
AGGGACATATCTCACAAGCA+AGG + chr8.1:20070177-20070196 MS.gene059966:CDS 45.0%
AGGTCTATGTGGCATAGCTA+TGG + chr8.1:20070197-20070216 MS.gene059966:CDS 45.0%
ATCATGGGGTAGCAATTGTG+GGG + chr8.1:20070064-20070083 MS.gene059966:CDS 45.0%
CAAGACTTCGAGTAACTGTG+TGG - chr8.1:20068861-20068880 None:intergenic 45.0%
CACAAGTTCTTGCTCAGACA+AGG - chr8.1:20069510-20069529 None:intergenic 45.0%
CTGATGCTAAGTCATTCTCC+TGG - chr8.1:20068798-20068817 None:intergenic 45.0%
CTTGTCTGAGCAAGAACTTG+TGG + chr8.1:20069508-20069527 MS.gene059966:CDS 45.0%
GAACTCTTGGGGAAGTGAAT+GGG + chr8.1:20070128-20070147 MS.gene059966:CDS 45.0%
GAATCATGGGGTAGCAATTG+TGG + chr8.1:20070062-20070081 MS.gene059966:CDS 45.0%
GCAGTGAATCAAGGATGCAA+CGG + chr8.1:20069542-20069561 MS.gene059966:CDS 45.0%
GTAAAGGATCAAGGCCAATG+CGG + chr8.1:20069147-20069166 MS.gene059966:CDS 45.0%
TCTATGTGGCATAGCTATGG+AGG + chr8.1:20070200-20070219 MS.gene059966:CDS 45.0%
TCTCACAAGCAAGGTCTATG+TGG + chr8.1:20070186-20070205 MS.gene059966:CDS 45.0%
TCTGTAGCTATTGATGCTGG+TGG + chr8.1:20069882-20069901 MS.gene059966:CDS 45.0%
TGAATCAAGGATGCAACGGT+GGG + chr8.1:20069546-20069565 MS.gene059966:CDS 45.0%
TGGACAGTGAAGAACTCTTG+GGG + chr8.1:20070117-20070136 MS.gene059966:CDS 45.0%
TGTGGGATTTGTACGAGAGA+TGG + chr8.1:20068827-20068846 MS.gene059966:CDS 45.0%
! CGTGGATTATCACATGACAG+TGG + chr8.1:20069051-20069070 MS.gene059966:CDS 45.0%
! GGAGTATTTACTGGACACTG+TGG + chr8.1:20070030-20070049 MS.gene059966:CDS 45.0%
! GTGGTACTGAGCTGAATCAT+GGG + chr8.1:20070049-20070068 MS.gene059966:CDS 45.0%
! GTTCCCTCTTTTGCAGCATA+AGG - chr8.1:20069632-20069651 None:intergenic 45.0%
! TGGTACTGAGCTGAATCATG+GGG + chr8.1:20070050-20070069 MS.gene059966:CDS 45.0%
! TGTGGTACTGAGCTGAATCA+TGG + chr8.1:20070048-20070067 MS.gene059966:CDS 45.0%
!! AAATTTATTAAGTATATATT+TGG - chr8.1:20069701-20069720 None:intergenic 5.0%
GGAAGTGAATGGGGAGAACA+GGG + chr8.1:20070138-20070157 MS.gene059966:CDS 50.0%
GGGTATGGAGCAACTCAAGA+TGG + chr8.1:20070084-20070103 MS.gene059966:CDS 50.0%
GTGAATCAAGGATGCAACGG+TGG + chr8.1:20069545-20069564 MS.gene059966:CDS 50.0%
TGCGACACTGCAGTGAATCA+AGG + chr8.1:20069533-20069552 MS.gene059966:CDS 50.0%
TGTTGGCAGGGACATTCTCA+TGG - chr8.1:20069821-20069840 None:intergenic 50.0%
! AGGAAGATAGGTGCTGTCAC+CGG + chr8.1:20069123-20069142 MS.gene059966:CDS 50.0%
!! AGGATGCAACGGTGGGTTAA+TGG + chr8.1:20069553-20069572 MS.gene059966:CDS 50.0%
!! TTGGCCTTGATCCTTTACCC+CGG - chr8.1:20069145-20069164 None:intergenic 50.0%
GGGAAGTGAATGGGGAGAAC+AGG + chr8.1:20070137-20070156 MS.gene059966:CDS 55.0%
GGGGTAGCAATTGTGGGGTA+TGG + chr8.1:20070069-20070088 MS.gene059966:CDS 55.0%
GTCACCGGGGTAAAGGATCA+AGG + chr8.1:20069138-20069157 MS.gene059966:CDS 55.0%
! GAAGATAGGTGCTGTCACCG+GGG + chr8.1:20069125-20069144 MS.gene059966:CDS 55.0%
! GGAAGATAGGTGCTGTCACC+GGG + chr8.1:20069124-20069143 MS.gene059966:CDS 55.0%
! AGGTGCTGTCACCGGGGTAA+AGG + chr8.1:20069131-20069150 MS.gene059966:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 20068721 20070290 20068721 ID=MS.gene059966
chr8.1 mRNA 20068721 20070290 20068721 ID=MS.gene059966.t1;Parent=MS.gene059966
chr8.1 exon 20068721 20069168 20068721 ID=MS.gene059966.t1.exon1;Parent=MS.gene059966.t1
chr8.1 CDS 20068721 20069168 20068721 ID=cds.MS.gene059966.t1;Parent=MS.gene059966.t1
chr8.1 exon 20069435 20069667 20069435 ID=MS.gene059966.t1.exon2;Parent=MS.gene059966.t1
chr8.1 CDS 20069435 20069667 20069435 ID=cds.MS.gene059966.t1;Parent=MS.gene059966.t1
chr8.1 exon 20069789 20069929 20069789 ID=MS.gene059966.t1.exon3;Parent=MS.gene059966.t1
chr8.1 CDS 20069789 20069929 20069789 ID=cds.MS.gene059966.t1;Parent=MS.gene059966.t1
chr8.1 exon 20070030 20070290 20070030 ID=MS.gene059966.t1.exon4;Parent=MS.gene059966.t1
chr8.1 CDS 20070030 20070290 20070030 ID=cds.MS.gene059966.t1;Parent=MS.gene059966.t1
Gene Sequence

>MS.gene059966

ATGGAAATGAAGAAGCTCTTGTTTGTTTCCTTATCCCTAGCTTTGATTTTAGGAATAGCTAAGAGCTTTGATTTCCAGGAGAATGACTTAGCATCAGAGAAAAGTTTGTGGGATTTGTACGAGAGATGGAGAAGTCACCACACAGTTACTCGAAGTCTTGATGAGAAGAATAATCGTTTTAATGTGTTCAAAGCCAATGTTATGCATGTCCACAATACCAATAAATTAGATAAGCCTTATAAGCTTAAGTTGAATAAGTTTGCTGACATGACAAATTATGAATTTAGGAGCATCTATGCTGATTCTAAGGTTAATCATCATAGAATGTTCCGTGGATTATCACATGACAGTGGACCGTTCATGTATGAGAATGTGGAGGATGTTCCTTCTTCGATTGATTGGAGGAAGATAGGTGCTGTCACCGGGGTAAAGGATCAAGGCCAATGCGGTAAGCTTACTTGTTGATTAAGTAATCACATACTATTTATAATTTGCGTAAAACATGTTTTTTGATATCTACAAAATACTCACCATGTTAGTTAAATCGCTTGAAGTTTGATAGTTAAGTTTGTTCTTCCACTTAGTCTGTATTTGATTATACGTTTGGATCCAAAATTGATCTGGAGAACTGGAGAAGTGAAATTGATTTTAACATGTTTGATTGTTCTGAAGTAGAATTGATTTGGTCTTTTGAATTGTTTCTAACTTGTATAGGTAGTTGCTGGGCTTTCTCAACAATTGTAGCTGTTGAAGGTATTAACCAAATCAAGACACAAAAACTAGTATCCTTGTCTGAGCAAGAACTTGTGGATTGCGACACTGCAGTGAATCAAGGATGCAACGGTGGGTTAATGGAAAATGCATTCGAGTTCATCAAGCAAAATGGCATAACAACAGAAACTAATTATCCTTATGCTGCAAAAGAGGGAACCTGTAATACACAAAAGGTGCACGCACTAGTCTATTTATAATTAGTACCAAATATATACTTAATAAATTTTATTTTGATTGACTTATGAGAAAATTTAAAGATCCTATTAAGTATTAACACATTTTCACTTGATGTAGGAGAATAAGCAAGCAGTGTCAATTGATGGCCATGAGAATGTCCCTGCCAACAATGAAGCATCATTGTTGAAAGCTGCTGCCAACCAACCTATTTCTGTAGCTATTGATGCTGGTGGATCTGATTTCCAGTTTTATTCAGAGGTAAAACTTATATATTTTTATCCCTTACACATTTATATATTTTTGTATAAATTTGTATATCACCTTGATCCCACTTTTAATTACAAAAATATAATTACAGGGAGTATTTACTGGACACTGTGGTACTGAGCTGAATCATGGGGTAGCAATTGTGGGGTATGGAGCAACTCAAGATGGAACAAAATATTGGACAGTGAAGAACTCTTGGGGAAGTGAATGGGGAGAACAGGGTTACATTAGAATGCAAAGGGACATATCTCACAAGCAAGGTCTATGTGGCATAGCTATGGAGGCTTCCTATCCAATCAAAAAGTCATCTGAAAACCCAACAAAAAGTTCACTTCCAAAAGATGAGCTTTAA
Protein sequence

>MS.gene059966.t1

MEMKKLLFVSLSLALILGIAKSFDFQENDLASEKSLWDLYERWRSHHTVTRSLDEKNNRFNVFKANVMHVHNTNKLDKPYKLKLNKFADMTNYEFRSIYADSKVNHHRMFRGLSHDSGPFMYENVEDVPSSIDWRKIGAVTGVKDQGQCGSCWAFSTIVAVEGINQIKTQKLVSLSEQELVDCDTAVNQGCNGGLMENAFEFIKQNGITTETNYPYAAKEGTCNTQKENKQAVSIDGHENVPANNEASLLKAAANQPISVAIDAGGSDFQFYSEGVFTGHCGTELNHGVAIVGYGATQDGTKYWTVKNSWGSEWGEQGYIRMQRDISHKQGLCGIAMEASYPIKKSSENPTKSSLPKDEL