AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003677

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003677.t1 MTR_4g071060 97.238 362 10 0 1 362 1 362 0.0 743
MS.gene003677.t1 MTR_1g023210 69.837 368 99 6 1 360 1 364 0.0 529
MS.gene003677.t1 MTR_4g073660 68.132 364 76 6 3 363 4 330 7.98e-177 494
MS.gene003677.t1 MTR_4g072340 67.935 368 76 7 1 363 1 331 1.28e-176 494
MS.gene003677.t1 MTR_4g072790 68.681 364 70 7 3 363 5 327 2.47e-176 493
MS.gene003677.t1 MTR_1g094135 53.352 358 158 5 10 360 15 370 1.50e-140 404
MS.gene003677.t1 MTR_1g107550 42.667 300 146 7 50 341 54 335 5.48e-68 221
MS.gene003677.t1 MTR_4g079770 38.310 355 178 13 5 348 12 336 2.22e-65 211
MS.gene003677.t1 MTR_4g047610 39.748 317 153 10 54 353 44 339 1.27e-64 209
MS.gene003677.t1 MTR_4g079470 39.329 328 168 12 32 348 29 336 1.51e-64 209
MS.gene003677.t1 MTR_3g116080 37.119 361 193 12 4 353 7 344 2.62e-63 206
MS.gene003677.t1 MTR_4g079800 38.750 320 168 11 45 353 39 341 2.98e-63 205
MS.gene003677.t1 MTR_4g125300 37.568 370 189 11 1 353 1 345 3.39e-63 205
MS.gene003677.t1 MTR_1g018840 37.978 366 177 12 1 341 1 341 4.45e-63 209
MS.gene003677.t1 MTR_1g018840 37.978 366 177 12 1 341 1 341 6.56e-63 207
MS.gene003677.t1 MTR_4g080700 37.421 318 174 8 38 348 32 331 8.81e-63 204
MS.gene003677.t1 MTR_5g022560 38.941 321 167 11 45 353 37 340 9.43e-63 204
MS.gene003677.t1 MTR_3g102220 36.467 351 187 11 8 341 10 341 9.85e-63 207
MS.gene003677.t1 MTR_4g094918 41.026 312 135 11 50 341 39 321 2.51e-62 203
MS.gene003677.t1 MTR_4g080730 37.417 302 166 7 54 348 46 331 1.01e-61 201
MS.gene003677.t1 MTR_4g079440 32.865 356 211 9 5 353 7 341 9.36e-61 199
MS.gene003677.t1 MTR_4g107930 38.907 311 164 9 54 353 46 341 3.53e-60 197
MS.gene003677.t1 MTR_3g112430 37.730 326 160 12 48 353 36 338 5.80e-59 197
MS.gene003677.t1 MTR_3g075390 35.574 357 189 11 7 348 6 336 1.43e-58 194
MS.gene003677.t1 MTR_4g080360 35.831 307 169 9 54 348 43 333 9.46e-58 191
MS.gene003677.t1 MTR_2g075830 37.086 302 169 6 54 348 45 332 1.02e-56 188
MS.gene003677.t1 MTR_4g104370 38.361 305 156 10 58 348 49 335 1.09e-56 189
MS.gene003677.t1 MTR_2g015850 39.437 284 149 8 64 341 78 344 1.36e-55 186
MS.gene003677.t1 MTR_4g065077 34.409 372 194 14 5 352 7 352 1.59e-55 186
MS.gene003677.t1 MTR_2g015920 32.796 372 194 14 8 350 8 352 1.13e-54 184
MS.gene003677.t1 MTR_2g090890 35.000 320 180 8 48 359 39 338 2.27e-54 182
MS.gene003677.t1 MTR_2g016180 35.514 321 173 11 48 353 51 352 2.77e-53 183
MS.gene003677.t1 MTR_2g083930 36.336 333 179 14 41 358 30 344 1.59e-52 177
MS.gene003677.t1 MTR_2g016270 35.110 319 162 11 48 353 51 337 1.62e-52 181
MS.gene003677.t1 MTR_8g086470 34.483 348 182 12 5 341 23 335 3.46e-52 177
MS.gene003677.t1 MTR_2g015930 31.129 363 209 12 6 349 11 351 9.88e-49 168
MS.gene003677.t1 MTR_7g078600 32.288 319 179 9 42 348 36 329 1.65e-46 162
MS.gene003677.t1 MTR_2g067900 33.333 333 181 13 42 353 10 322 2.02e-44 156
MS.gene003677.t1 MTR_7g079230 31.661 319 182 9 42 348 33 327 8.33e-43 152
MS.gene003677.t1 MTR_8g087510 50.000 136 57 3 172 306 23 148 6.33e-41 142
MS.gene003677.t1 MTR_1g023810 40.991 222 111 7 129 347 47 251 1.61e-40 144
MS.gene003677.t1 MTR_2g064475 31.165 369 176 15 44 362 12 352 1.02e-39 144
MS.gene003677.t1 MTR_2g067560 33.639 327 164 13 42 341 10 310 7.37e-38 144
MS.gene003677.t1 MTR_2g066980 28.158 380 183 16 1 355 1 315 1.47e-32 124
MS.gene003677.t1 MTR_7g111050 30.000 290 152 14 95 353 63 332 1.58e-23 100
MS.gene003677.t1 MTR_7g078630 27.206 272 154 5 48 308 706 944 1.71e-23 102
MS.gene003677.t1 MTR_7g111060 29.268 287 158 12 95 353 66 335 1.48e-22 97.8
MS.gene003677.t1 MTR_1g025450 38.043 184 85 7 126 306 50 207 7.72e-22 97.1
MS.gene003677.t1 MTR_2g015840 36.879 141 79 3 54 184 39 179 2.67e-19 87.0
MS.gene003677.t1 MTR_2g015840 34.746 118 69 3 244 360 161 271 1.16e-13 70.9
MS.gene003677.t1 MTR_2g047960 42.391 92 45 1 135 226 2 85 4.56e-15 70.9
MS.gene003677.t1 MTR_7g078310 41.584 101 50 2 130 228 151 244 4.62e-15 74.7
MS.gene003677.t1 MTR_3g029560 27.737 274 139 14 104 358 4 237 1.12e-12 67.4
MS.gene003677.t1 MTR_7g111050 29.098 244 128 13 95 307 58 287 1.03e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003677.t1 AT2G21430 73.611 360 92 3 1 358 1 359 0.0 558
MS.gene003677.t1 AT4G39090 73.109 357 94 2 8 362 10 366 0.0 552
MS.gene003677.t1 AT4G16190 67.292 373 112 4 1 363 1 373 0.0 509
MS.gene003677.t1 AT3G54940 58.172 361 134 7 6 356 9 362 1.38e-152 434
MS.gene003677.t1 AT3G54940 53.455 275 112 6 6 271 9 276 7.13e-99 295
MS.gene003677.t1 AT3G19400 40.968 310 153 9 57 353 53 345 5.70e-67 216
MS.gene003677.t1 AT3G45310 38.483 356 184 11 10 348 11 348 1.93e-66 214
MS.gene003677.t1 AT5G60360 39.039 333 175 9 31 353 39 353 2.81e-66 214
MS.gene003677.t1 AT3G19390 38.504 361 181 12 8 353 8 342 1.84e-65 214
MS.gene003677.t1 AT5G60360 39.450 327 170 9 31 347 39 347 3.51e-65 211
MS.gene003677.t1 AT3G45310 38.136 354 184 11 10 346 11 346 4.73e-65 210
MS.gene003677.t1 AT5G60360 39.264 326 170 9 31 346 39 346 8.34e-65 210
MS.gene003677.t1 AT1G20850 39.117 317 165 8 48 353 51 350 2.11e-64 209
MS.gene003677.t1 AT1G09850 39.198 324 166 11 48 359 32 336 3.63e-64 211
MS.gene003677.t1 AT1G47128 37.971 345 183 8 9 341 8 333 2.30e-63 209
MS.gene003677.t1 AT5G43060 42.199 282 142 7 64 341 70 334 5.52e-63 208
MS.gene003677.t1 AT1G29090 39.564 321 160 12 44 348 44 346 1.44e-62 204
MS.gene003677.t1 AT5G45890 34.153 366 192 10 1 348 3 337 5.85e-62 202
MS.gene003677.t1 AT4G35350 37.025 316 172 8 48 353 51 349 3.17e-61 201
MS.gene003677.t1 AT1G06260 37.846 325 161 13 45 353 40 339 5.27e-61 199
MS.gene003677.t1 AT3G43960 40.614 293 153 7 56 341 49 327 8.95e-61 200
MS.gene003677.t1 AT4G36880 38.754 289 150 6 64 341 69 341 4.25e-59 196
MS.gene003677.t1 AT3G48340 36.494 348 179 11 9 341 4 324 4.01e-58 192
MS.gene003677.t1 AT2G27420 36.278 317 162 11 54 348 41 339 2.35e-57 190
MS.gene003677.t1 AT4G23520 33.424 368 195 14 5 353 10 346 2.53e-57 190
MS.gene003677.t1 AT5G50260 39.744 312 143 11 58 348 47 334 9.90e-57 189
MS.gene003677.t1 AT1G29080 36.943 314 168 9 54 353 45 342 1.14e-54 183
MS.gene003677.t1 AT3G48350 34.857 350 187 11 7 342 3 325 1.27e-54 184
MS.gene003677.t1 AT4G11310 32.591 359 203 10 6 348 9 344 1.28e-52 178
MS.gene003677.t1 AT2G34080 35.691 311 165 11 54 348 45 336 4.00e-52 176
MS.gene003677.t1 AT4G11320 34.921 315 172 8 48 348 56 351 1.06e-51 176
MS.gene003677.t1 AT4G11320 34.921 315 172 8 48 348 56 351 1.06e-51 176
MS.gene003677.t1 AT3G49340 33.866 313 174 11 54 351 41 335 2.17e-51 174
MS.gene003677.t1 AT3G19400 40.889 225 121 4 57 276 53 270 1.48e-50 171
MS.gene003677.t1 AT4G35350 36.052 233 136 4 48 276 51 274 9.24e-46 158
MS.gene003677.t1 AT1G29110 32.154 311 166 9 54 348 44 325 2.26e-42 150
MS.gene003677.t1 AT3G48350 32.787 305 155 10 7 287 3 281 9.20e-39 140
MS.gene003677.t1 AT4G01610 29.874 318 168 14 61 349 40 331 1.51e-23 100
MS.gene003677.t1 AT1G02305 30.556 288 151 15 95 352 69 337 1.31e-22 97.8
MS.gene003677.t1 AT4G01610 29.560 318 169 14 61 349 40 331 2.31e-22 97.1
MS.gene003677.t1 AT1G02300 27.508 309 153 16 95 352 66 354 3.95e-18 85.1

Find 87 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATATATACTGCATTTA+AGG 0.130654 4.1:-31790039 None:intergenic
AACCTATTTAAACATAAGTT+TGG 0.234066 4.1:+31788699 MS.gene003677:CDS
TCTCTCCAATCAAAATCTTT+TGG 0.234300 4.1:-31788948 None:intergenic
TCCCAAACTTATGTTTAAAT+AGG 0.268902 4.1:-31788701 None:intergenic
CCAAGGATGAACATGATTAC+AGG 0.304516 4.1:+31788736 MS.gene003677:CDS
GGTGAGACTTGGGGAGAAAA+TGG 0.310928 4.1:+31790203 MS.gene003677:CDS
CTGGAGCCTATTCGCCTATT+AGG 0.320950 4.1:+31790141 MS.gene003677:CDS
GGAGTTTCGTCAGAGCGTTT+TGG 0.321592 4.1:+31788860 MS.gene003677:CDS
CCACGATCAGTAGCAGAATA+AGG 0.357295 4.1:-31789796 None:intergenic
ACTAGATCATGGAGTGTTAC+TGG 0.371273 4.1:+31790106 MS.gene003677:CDS
ACCTATTTAAACATAAGTTT+GGG 0.393244 4.1:+31788700 MS.gene003677:CDS
AGTCTCTTTGAGCATATGTA+TGG 0.401014 4.1:-31790086 None:intergenic
AGATTATCTGTGGGAAGAAT+CGG 0.407382 4.1:-31788924 None:intergenic
GGCTTCTCCTTCATCCTAAT+AGG 0.407412 4.1:-31790155 None:intergenic
TCACAAGAACCCGGTTGCTC+TGG 0.408096 4.1:-31789691 None:intergenic
GGAGTGTTACTGGTGGGATA+TGG 0.410428 4.1:+31790116 MS.gene003677:CDS
ATTGAATCTTCTCCGTCATC+CGG 0.411609 4.1:-31788621 None:intergenic
GGGGCTGCATTAGCATCTTT+CGG 0.417402 4.1:-31788903 None:intergenic
GGATGAAGGAGAAGCCATAT+TGG 0.423435 4.1:+31790162 MS.gene003677:CDS
TGCTAAGAAGCATCAATTGA+TGG 0.425569 4.1:+31788788 MS.gene003677:CDS
GATTATCTGTGGGAAGAATC+GGG 0.426123 4.1:-31788923 None:intergenic
CTTCATCCTAATAGGCGAAT+AGG 0.432818 4.1:-31790147 None:intergenic
TGACAAGATTAGCGGCAATC+TGG 0.437809 4.1:-31789891 None:intergenic
CAACCGGGTTCTTGTGATGC+CGG 0.438520 4.1:+31789697 MS.gene003677:CDS
ATAGTGTGATCCAGAGCAAC+CGG 0.450276 4.1:+31789681 MS.gene003677:intron
TCTTGTGATGCCGGGTGCAA+TGG 0.456716 4.1:+31789706 MS.gene003677:CDS
CCTGTAATCATGTTCATCCT+TGG 0.456777 4.1:-31788736 None:intergenic
AAAACTCATGGGGTGAGACT+TGG 0.466256 4.1:+31790192 MS.gene003677:CDS
GCATCAATTGATGGATCCTT+CGG 0.466749 4.1:+31788797 MS.gene003677:CDS
TGTTGGAGTTTCAGTACAAC+CGG 0.469204 4.1:+31789127 MS.gene003677:CDS
ATGGATCCTTCGGCGGTTCA+CGG 0.470073 4.1:+31788807 MS.gene003677:CDS
AAACTCATGGGGTGAGACTT+GGG 0.470356 4.1:+31790193 MS.gene003677:CDS
CGACAGGTGGTCGACGATGA+AGG 0.470944 4.1:+31788648 MS.gene003677:CDS
ATGGATACTACAAAATATGC+AGG 0.476628 4.1:+31790222 MS.gene003677:CDS
GACGATGAAGGAGTGCGGTT+GGG 0.489192 4.1:+31788660 MS.gene003677:CDS
AACCGGGTTCTTGTGATGCC+GGG 0.494687 4.1:+31789698 MS.gene003677:CDS
TTACTGGTGGGATATGGATC+TGG 0.507620 4.1:+31790122 MS.gene003677:CDS
TCAATTGATGCTTCTTAGCA+CGG 0.511562 4.1:-31788785 None:intergenic
TGGGAAGGTTTATTCTTCCA+AGG 0.519917 4.1:+31788719 MS.gene003677:CDS
GGTGCTCACTTTCTCTCGAC+CGG 0.523916 4.1:+31789160 MS.gene003677:CDS
CGACGATGAAGGAGTGCGGT+TGG 0.531274 4.1:+31788659 MS.gene003677:CDS
CCTTATTCTGCTACTGATCG+TGG 0.532697 4.1:+31789796 MS.gene003677:CDS
AGGGCTCGTGTGGGTCATGT+TGG 0.536407 4.1:+31789110 MS.gene003677:intron
CTTTAGCACTTACTAGCAAG+AGG 0.547021 4.1:-31789922 None:intergenic
ACCGTTCTTGACAAGATTAG+CGG 0.547122 4.1:-31789899 None:intergenic
AGTACAACCGGTGCACTTGA+AGG 0.552820 4.1:+31789139 MS.gene003677:CDS
AAACTCCCTCGGAGTAAGAT+CGG 0.553683 4.1:-31788844 None:intergenic
TTCTTCGCGCATCACCCCAC+CGG 0.555594 4.1:-31789767 None:intergenic
CATCAGACCACCATTGCACC+CGG 0.562811 4.1:-31789716 None:intergenic
CTTCATCGTCGACCACCTGT+CGG 0.563999 4.1:-31788647 None:intergenic
CTATTCGCCTATTAGGATGA+AGG 0.564118 4.1:+31790148 MS.gene003677:CDS
TGCTCAAAGAGACTAGATCA+TGG 0.566764 4.1:+31790095 MS.gene003677:CDS
AGAAGATTCAATAATCCGAC+AGG 0.575409 4.1:+31788632 MS.gene003677:CDS
TGCAGGGGACGAAATATATG+TGG 0.578645 4.1:+31790239 MS.gene003677:CDS
GTCTCTTTGAGCATATGTAT+GGG 0.587803 4.1:-31790085 None:intergenic
ATTTAAACATAAGTTTGGGA+AGG 0.587990 4.1:+31788704 MS.gene003677:CDS
GCCGCTAATCTTGTCAAGAA+CGG 0.589186 4.1:+31789898 MS.gene003677:CDS
GTGAGCACCTTCAAGTGCAC+CGG 0.592969 4.1:-31789146 None:intergenic
AACTCATGGGGTGAGACTTG+GGG 0.593059 4.1:+31790194 MS.gene003677:CDS
ACTTGTCGATTGTGATCATG+AGG 0.595475 4.1:+31789210 MS.gene003677:CDS
TATATGTGGAGTGGACTCCA+TGG 0.597639 4.1:+31790253 MS.gene003677:CDS
TGGATACTACAAAATATGCA+GGG 0.599253 4.1:+31790223 MS.gene003677:CDS
GTATATATGCAAACATACAT+TGG 0.601664 4.1:+31790050 MS.gene003677:CDS
TGTGATGCCGGGTGCAATGG+TGG 0.601772 4.1:+31789709 MS.gene003677:CDS
ACGCTCTGACGAAACTCCCT+CGG 0.605558 4.1:-31788855 None:intergenic
ATTCTCCGATCTTACTCCGA+GGG 0.607597 4.1:+31788839 MS.gene003677:CDS
CACCCGGCATCACAAGAACC+CGG 0.609271 4.1:-31789700 None:intergenic
TATATGCAAACATACATTGG+AGG 0.612222 4.1:+31790053 MS.gene003677:CDS
GCATTCTCTACGCCGGATGA+CGG 0.614941 4.1:+31788609 MS.gene003677:CDS
GGACTCCATGGTTTCTACTG+TGG 0.626354 4.1:+31790265 MS.gene003677:CDS
CGGCGTAGAGAATGCCGAGA+CGG 0.629708 4.1:-31788601 None:intergenic
TCAATTGATGGATCCTTCGG+CGG 0.633662 4.1:+31788800 MS.gene003677:CDS
GATCATGGAGTGTTACTGGT+GGG 0.636411 4.1:+31790110 MS.gene003677:CDS
GGGACGAAATATATGTGGAG+TGG 0.637649 4.1:+31790244 MS.gene003677:CDS
GTAACACCGTGAACCGCCGA+AGG 0.638844 4.1:-31788813 None:intergenic
TAGTGTGATCCAGAGCAACC+GGG 0.639888 4.1:+31789682 MS.gene003677:intron
TTACTGAGTAGTAGTATGAA+CGG 0.642920 4.1:-31790291 None:intergenic
CGTCTCGGCATTCTCTACGC+CGG 0.649156 4.1:+31788602 MS.gene003677:CDS
TAATGATTCAGGGCTCGTGT+GGG 0.650844 4.1:+31789101 MS.gene003677:intron
GGCAGCCACAGTAGAAACCA+TGG 0.655273 4.1:-31790270 None:intergenic
ATAATGATTCAGGGCTCGTG+TGG 0.664587 4.1:+31789100 MS.gene003677:intron
AGATCATGGAGTGTTACTGG+TGG 0.667806 4.1:+31790109 MS.gene003677:CDS
GGATACTACAAAATATGCAG+GGG 0.670950 4.1:+31790224 MS.gene003677:CDS
AGATTCAATAATCCGACAGG+TGG 0.672731 4.1:+31788635 MS.gene003677:CDS
ATTATCTGTGGGAAGAATCG+GGG 0.677726 4.1:-31788922 None:intergenic
AATTCTCCGATCTTACTCCG+AGG 0.702571 4.1:+31788838 MS.gene003677:CDS
TGGTCGACGATGAAGGAGTG+CGG 0.713653 4.1:+31788655 MS.gene003677:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTTTACAAAATTATTTAT+TGG + chr4.1:31789568-31789587 MS.gene003677:intron 10.0%
!! TCTTTACAAAATTATTTATT+GGG + chr4.1:31789569-31789588 MS.gene003677:intron 10.0%
!! TTATTATATGTTATCTAAAT+TGG + chr4.1:31789634-31789653 MS.gene003677:intron 10.0%
!! AGATATACTATTAATTACAA+AGG - chr4.1:31789259-31789278 None:intergenic 15.0%
!! ATAATAATCATAATCACAAT+TGG - chr4.1:31789014-31789033 None:intergenic 15.0%
!! GATATACTATTAATTACAAA+GGG - chr4.1:31789258-31789277 None:intergenic 15.0%
!! AACCTATTTAAACATAAGTT+TGG + chr4.1:31788699-31788718 MS.gene003677:CDS 20.0%
!! AATTAATAGTATATCTGCTA+AGG + chr4.1:31789263-31789282 MS.gene003677:intron 20.0%
!! ACCTATTTAAACATAAGTTT+GGG + chr4.1:31788700-31788719 MS.gene003677:CDS 20.0%
!! ATACAATATCTAATAGAGTT+TGG - chr4.1:31789403-31789422 None:intergenic 20.0%
!! ATATGTTATCTAAATTGGTT+TGG + chr4.1:31789639-31789658 MS.gene003677:intron 20.0%
!! ATTAATTACAAAGGGAAAAT+AGG - chr4.1:31789250-31789269 None:intergenic 20.0%
!! ATTGGATAATTAAAAACTCA+TGG + chr4.1:31790180-31790199 MS.gene003677:CDS 20.0%
!! TATCTGATATTAATCTTTCT+AGG + chr4.1:31789347-31789366 MS.gene003677:intron 20.0%
!! TTGGATAATTAAAAACTCAT+GGG + chr4.1:31790181-31790200 MS.gene003677:CDS 20.0%
!!! AAAACAAATTTTGATTGTGT+TGG + chr4.1:31789070-31789089 MS.gene003677:intron 20.0%
!!! AAACAAATTTTGATTGTGTT+GGG + chr4.1:31789071-31789090 MS.gene003677:intron 20.0%
!!! AAATGCTAACTTGTAATATT+TGG + chr4.1:31789592-31789611 MS.gene003677:intron 20.0%
!!! AATGCTAACTTGTAATATTT+GGG + chr4.1:31789593-31789612 MS.gene003677:intron 20.0%
!!! GAGTTTTTAATTATCCAATA+TGG - chr4.1:31790179-31790198 None:intergenic 20.0%
!!! TTTGAGTACATTTTAAAATC+CGG + chr4.1:31789748-31789767 MS.gene003677:CDS 20.0%
! ACTCTATTAGATATTGTATG+AGG + chr4.1:31789404-31789423 MS.gene003677:intron 25.0%
! ATTTAAACATAAGTTTGGGA+AGG + chr4.1:31788704-31788723 MS.gene003677:CDS 25.0%
! GTATATATGCAAACATACAT+TGG + chr4.1:31790050-31790069 MS.gene003677:CDS 25.0%
! TCCCAAACTTATGTTTAAAT+AGG - chr4.1:31788704-31788723 None:intergenic 25.0%
! TGGATAATTAAAAACTCATG+GGG + chr4.1:31790182-31790201 MS.gene003677:CDS 25.0%
! TTGCATATATACTGCATTTA+AGG - chr4.1:31790042-31790061 None:intergenic 25.0%
!! AGCAAATTTGAAACATGATT+AGG + chr4.1:31789500-31789519 MS.gene003677:intron 25.0%
!! ATGCTAACTTGTAATATTTG+GGG + chr4.1:31789594-31789613 MS.gene003677:intron 25.0%
!!! ATCTTCCAAAAGATTTTGAT+TGG + chr4.1:31788943-31788962 MS.gene003677:CDS 25.0%
!!! TTATCTAAATTGGTTTGGAT+TGG + chr4.1:31789644-31789663 MS.gene003677:intron 25.0%
AGTTACTGCTGTTAAAAATC+AGG + chr4.1:31788980-31788999 MS.gene003677:CDS 30.0%
ATGGATACTACAAAATATGC+AGG + chr4.1:31790222-31790241 MS.gene003677:CDS 30.0%
TATATGCAAACATACATTGG+AGG + chr4.1:31790053-31790072 MS.gene003677:CDS 30.0%
TGGATACTACAAAATATGCA+GGG + chr4.1:31790223-31790242 MS.gene003677:CDS 30.0%
TTACTGAGTAGTAGTATGAA+CGG - chr4.1:31790294-31790313 None:intergenic 30.0%
! TCTCTCCAATCAAAATCTTT+TGG - chr4.1:31788951-31788970 None:intergenic 30.0%
!! ATCTTTTGGAAGATTATCTG+TGG - chr4.1:31788937-31788956 None:intergenic 30.0%
!! TCTTTTGGAAGATTATCTGT+GGG - chr4.1:31788936-31788955 None:intergenic 30.0%
!!! AGTACATTTTAAAATCCGGT+GGG + chr4.1:31789752-31789771 MS.gene003677:CDS 30.0%
AGAAGATTCAATAATCCGAC+AGG + chr4.1:31788632-31788651 MS.gene003677:CDS 35.0%
AGATTATCTGTGGGAAGAAT+CGG - chr4.1:31788927-31788946 None:intergenic 35.0%
AGTCTCTTTGAGCATATGTA+TGG - chr4.1:31790089-31790108 None:intergenic 35.0%
ATTGTATGAGGTTCGACTTA+AGG + chr4.1:31789416-31789435 MS.gene003677:intron 35.0%
GGATACTACAAAATATGCAG+GGG + chr4.1:31790224-31790243 MS.gene003677:CDS 35.0%
GTCTCTTTGAGCATATGTAT+GGG - chr4.1:31790088-31790107 None:intergenic 35.0%
TCAATTGATGCTTCTTAGCA+CGG - chr4.1:31788788-31788807 None:intergenic 35.0%
! GGGTTTGTGATAATGATTCA+GGG + chr4.1:31789091-31789110 MS.gene003677:intron 35.0%
! TGGGTTTGTGATAATGATTC+AGG + chr4.1:31789090-31789109 MS.gene003677:intron 35.0%
!! TGCTAAGAAGCATCAATTGA+TGG + chr4.1:31788788-31788807 MS.gene003677:CDS 35.0%
!!! GAGTACATTTTAAAATCCGG+TGG + chr4.1:31789751-31789770 MS.gene003677:CDS 35.0%
!!! GATTTTGATTGGAGAGAGAA+AGG + chr4.1:31788954-31788973 MS.gene003677:CDS 35.0%
!!! GTACATTTTAAAATCCGGTG+GGG + chr4.1:31789753-31789772 MS.gene003677:CDS 35.0%
ACCGTTCTTGACAAGATTAG+CGG - chr4.1:31789902-31789921 None:intergenic 40.0%
AGATTCAATAATCCGACAGG+TGG + chr4.1:31788635-31788654 MS.gene003677:CDS 40.0%
ATTATCTGTGGGAAGAATCG+GGG - chr4.1:31788925-31788944 None:intergenic 40.0%
ATTGAATCTTCTCCGTCATC+CGG - chr4.1:31788624-31788643 None:intergenic 40.0%
CCAAGGATGAACATGATTAC+AGG + chr4.1:31788736-31788755 MS.gene003677:CDS 40.0%
CCTGTAATCATGTTCATCCT+TGG - chr4.1:31788739-31788758 None:intergenic 40.0%
CTATTCGCCTATTAGGATGA+AGG + chr4.1:31790148-31790167 MS.gene003677:CDS 40.0%
CTTCATCCTAATAGGCGAAT+AGG - chr4.1:31790150-31790169 None:intergenic 40.0%
CTTTAGCACTTACTAGCAAG+AGG - chr4.1:31789925-31789944 None:intergenic 40.0%
GATTATCTGTGGGAAGAATC+GGG - chr4.1:31788926-31788945 None:intergenic 40.0%
TGCTCAAAGAGACTAGATCA+TGG + chr4.1:31790095-31790114 MS.gene003677:CDS 40.0%
TGGGAAGGTTTATTCTTCCA+AGG + chr4.1:31788719-31788738 MS.gene003677:CDS 40.0%
TGTTGGAGTTTCAGTACAAC+CGG + chr4.1:31789127-31789146 MS.gene003677:CDS 40.0%
! ACTAGATCATGGAGTGTTAC+TGG + chr4.1:31790106-31790125 MS.gene003677:CDS 40.0%
! ACTTGTCGATTGTGATCATG+AGG + chr4.1:31789210-31789229 MS.gene003677:CDS 40.0%
! GCATCAATTGATGGATCCTT+CGG + chr4.1:31788797-31788816 MS.gene003677:CDS 40.0%
! GCTAAGACTGACAAGTTTTC+CGG - chr4.1:31789182-31789201 None:intergenic 40.0%
! TCTTTTCATCTTCTCCGTCT+CGG + chr4.1:31788587-31788606 MS.gene003677:CDS 40.0%
AAAACTCATGGGGTGAGACT+TGG + chr4.1:31790192-31790211 MS.gene003677:CDS 45.0%
AAACTCATGGGGTGAGACTT+GGG + chr4.1:31790193-31790212 MS.gene003677:CDS 45.0%
AAACTCCCTCGGAGTAAGAT+CGG - chr4.1:31788847-31788866 None:intergenic 45.0%
AATTCTCCGATCTTACTCCG+AGG + chr4.1:31788838-31788857 MS.gene003677:CDS 45.0%
ATAATGATTCAGGGCTCGTG+TGG + chr4.1:31789100-31789119 MS.gene003677:intron 45.0%
ATAGTGTGATCCAGAGCAAC+CGG + chr4.1:31789681-31789700 MS.gene003677:intron 45.0%
ATTCTCCGATCTTACTCCGA+GGG + chr4.1:31788839-31788858 MS.gene003677:CDS 45.0%
CCACGATCAGTAGCAGAATA+AGG - chr4.1:31789799-31789818 None:intergenic 45.0%
CCTTATTCTGCTACTGATCG+TGG + chr4.1:31789796-31789815 MS.gene003677:CDS 45.0%
GCCGCTAATCTTGTCAAGAA+CGG + chr4.1:31789898-31789917 MS.gene003677:CDS 45.0%
GGATGAAGGAGAAGCCATAT+TGG + chr4.1:31790162-31790181 MS.gene003677:CDS 45.0%
GGCTTCTCCTTCATCCTAAT+AGG - chr4.1:31790158-31790177 None:intergenic 45.0%
GGGACGAAATATATGTGGAG+TGG + chr4.1:31790244-31790263 MS.gene003677:CDS 45.0%
TAATGATTCAGGGCTCGTGT+GGG + chr4.1:31789101-31789120 MS.gene003677:intron 45.0%
TGACAAGATTAGCGGCAATC+TGG - chr4.1:31789894-31789913 None:intergenic 45.0%
TGCAGGGGACGAAATATATG+TGG + chr4.1:31790239-31790258 MS.gene003677:CDS 45.0%
TTACTGGTGGGATATGGATC+TGG + chr4.1:31790122-31790141 MS.gene003677:CDS 45.0%
! AGATCATGGAGTGTTACTGG+TGG + chr4.1:31790109-31790128 MS.gene003677:CDS 45.0%
! GATCATGGAGTGTTACTGGT+GGG + chr4.1:31790110-31790129 MS.gene003677:CDS 45.0%
! GCACATCGATAGTGACTTGT+AGG + chr4.1:31789452-31789471 MS.gene003677:intron 45.0%
! TATATGTGGAGTGGACTCCA+TGG + chr4.1:31790253-31790272 MS.gene003677:CDS 45.0%
! TCAATTGATGGATCCTTCGG+CGG + chr4.1:31788800-31788819 MS.gene003677:CDS 45.0%
!! GAGTTTCGTCAGAGCGTTTT+GGG + chr4.1:31788861-31788880 MS.gene003677:CDS 45.0%
!!! AGAGCGTTTTGGGATTGAGA+GGG + chr4.1:31788871-31788890 MS.gene003677:CDS 45.0%
AACTCATGGGGTGAGACTTG+GGG + chr4.1:31790194-31790213 MS.gene003677:CDS 50.0%
AGTACAACCGGTGCACTTGA+AGG + chr4.1:31789139-31789158 MS.gene003677:CDS 50.0%
CTGGAGCCTATTCGCCTATT+AGG + chr4.1:31790141-31790160 MS.gene003677:CDS 50.0%
GGACTCCATGGTTTCTACTG+TGG + chr4.1:31790265-31790284 MS.gene003677:CDS 50.0%
GGGGCTGCATTAGCATCTTT+CGG - chr4.1:31788906-31788925 None:intergenic 50.0%
GGTGAGACTTGGGGAGAAAA+TGG + chr4.1:31790203-31790222 MS.gene003677:CDS 50.0%
TAGTGTGATCCAGAGCAACC+GGG + chr4.1:31789682-31789701 MS.gene003677:intron 50.0%
! GGAGTGTTACTGGTGGGATA+TGG + chr4.1:31790116-31790135 MS.gene003677:CDS 50.0%
!! GGAGTTTCGTCAGAGCGTTT+TGG + chr4.1:31788860-31788879 MS.gene003677:CDS 50.0%
!!! CAGAGCGTTTTGGGATTGAG+AGG + chr4.1:31788870-31788889 MS.gene003677:CDS 50.0%
!!! GAGCGTTTTGGGATTGAGAG+GGG + chr4.1:31788872-31788891 MS.gene003677:CDS 50.0%
!!! GTTTTGGGATTGAGAGGGGT+GGG + chr4.1:31788876-31788895 MS.gene003677:CDS 50.0%
!!! TTTTGGGATTGAGAGGGGTG+GGG + chr4.1:31788877-31788896 MS.gene003677:CDS 50.0%
AACCGGGTTCTTGTGATGCC+GGG + chr4.1:31789698-31789717 MS.gene003677:CDS 55.0%
ACGCTCTGACGAAACTCCCT+CGG - chr4.1:31788858-31788877 None:intergenic 55.0%
ATGGATCCTTCGGCGGTTCA+CGG + chr4.1:31788807-31788826 MS.gene003677:CDS 55.0%
CAACCGGGTTCTTGTGATGC+CGG + chr4.1:31789697-31789716 MS.gene003677:CDS 55.0%
CATCAGACCACCATTGCACC+CGG - chr4.1:31789719-31789738 None:intergenic 55.0%
CTTCATCGTCGACCACCTGT+CGG - chr4.1:31788650-31788669 None:intergenic 55.0%
GCATTCTCTACGCCGGATGA+CGG + chr4.1:31788609-31788628 MS.gene003677:CDS 55.0%
GGCAGCCACAGTAGAAACCA+TGG - chr4.1:31790273-31790292 None:intergenic 55.0%
GGTGCTCACTTTCTCTCGAC+CGG + chr4.1:31789160-31789179 MS.gene003677:CDS 55.0%
GTGAGCACCTTCAAGTGCAC+CGG - chr4.1:31789149-31789168 None:intergenic 55.0%
TCACAAGAACCCGGTTGCTC+TGG - chr4.1:31789694-31789713 None:intergenic 55.0%
TCTTGTGATGCCGGGTGCAA+TGG + chr4.1:31789706-31789725 MS.gene003677:CDS 55.0%
TGGTCGACGATGAAGGAGTG+CGG + chr4.1:31788655-31788674 MS.gene003677:CDS 55.0%
! GACGATGAAGGAGTGCGGTT+GGG + chr4.1:31788660-31788679 MS.gene003677:CDS 55.0%
!!! CGTTTTGGGATTGAGAGGGG+TGG + chr4.1:31788875-31788894 MS.gene003677:CDS 55.0%
AGGGCTCGTGTGGGTCATGT+TGG + chr4.1:31789110-31789129 MS.gene003677:intron 60.0%
CACCCGGCATCACAAGAACC+CGG - chr4.1:31789703-31789722 None:intergenic 60.0%
CGACAGGTGGTCGACGATGA+AGG + chr4.1:31788648-31788667 MS.gene003677:CDS 60.0%
CGGCGTAGAGAATGCCGAGA+CGG - chr4.1:31788604-31788623 None:intergenic 60.0%
CGTCTCGGCATTCTCTACGC+CGG + chr4.1:31788602-31788621 MS.gene003677:CDS 60.0%
GTAACACCGTGAACCGCCGA+AGG - chr4.1:31788816-31788835 None:intergenic 60.0%
TGTGATGCCGGGTGCAATGG+TGG + chr4.1:31789709-31789728 MS.gene003677:CDS 60.0%
TTCTTCGCGCATCACCCCAC+CGG - chr4.1:31789770-31789789 None:intergenic 60.0%
! CGACGATGAAGGAGTGCGGT+TGG + chr4.1:31788659-31788678 MS.gene003677:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 31788555 31790313 31788555 ID=MS.gene003677
chr4.1 mRNA 31788555 31790313 31788555 ID=MS.gene003677.t1;Parent=MS.gene003677
chr4.1 exon 31788555 31789001 31788555 ID=MS.gene003677.t1.exon1;Parent=MS.gene003677.t1
chr4.1 CDS 31788555 31789001 31788555 ID=cds.MS.gene003677.t1;Parent=MS.gene003677.t1
chr4.1 exon 31789112 31789231 31789112 ID=MS.gene003677.t1.exon2;Parent=MS.gene003677.t1
chr4.1 CDS 31789112 31789231 31789112 ID=cds.MS.gene003677.t1;Parent=MS.gene003677.t1
chr4.1 exon 31789685 31789931 31789685 ID=MS.gene003677.t1.exon3;Parent=MS.gene003677.t1
chr4.1 CDS 31789685 31789931 31789685 ID=cds.MS.gene003677.t1;Parent=MS.gene003677.t1
chr4.1 exon 31790036 31790313 31790036 ID=MS.gene003677.t1.exon4;Parent=MS.gene003677.t1
chr4.1 CDS 31790036 31790313 31790036 ID=cds.MS.gene003677.t1;Parent=MS.gene003677.t1
Gene Sequence

>MS.gene003677

ATGGATCATCGCACTCTTCTTCTCTTCGTCGTTCTTTTCATCTTCTCCGTCTCGGCATTCTCTACGCCGGATGACGGAGAAGATTCAATAATCCGACAGGTGGTCGACGATGAAGGAGTGCGGTTGGGAGCAGAGCATCACTTTAACCTATTTAAACATAAGTTTGGGAAGGTTTATTCTTCCAAGGATGAACATGATTACAGGTTTAAAATCTTCAAGTCTAATCTGAACCGTGCTAAGAAGCATCAATTGATGGATCCTTCGGCGGTTCACGGTGTTACGAAATTCTCCGATCTTACTCCGAGGGAGTTTCGTCAGAGCGTTTTGGGATTGAGAGGGGTGGGGTTGCCGAAAGATGCTAATGCAGCCCCGATTCTTCCCACAGATAATCTTCCAAAAGATTTTGATTGGAGAGAGAAAGGAGCAGTTACTGCTGTTAAAAATCAGGTATTGTTTCCAATTGTGATTATGATTATTATTTGTTTATTATTATTGTTTCGTTTAAAAAAAAAAAAAAAACAAATTTTGATTGTGTTGGGTTTGTGATAATGATTCAGGGCTCGTGTGGGTCATGTTGGAGTTTCAGTACAACCGGTGCACTTGAAGGTGCTCACTTTCTCTCGACCGGAAAACTTGTCAGTCTTAGCGAGCAACAACTTGTCGATTGTGATCATGAGGTTTTTTCTTCTTTTCCTATTTTCCCTTTGTAATTAATAGTATATCTGCTAAGGAATTATGATTATGATTATAATTGTGAATTGTGATTGTCATACTGCATTTTGTAAAGAACTTTATCTGATATTAATCTTTCTAGGAAAACAAGTAACAAAAATAACATTTAGTATCCAAACTCTATTAGATATTGTATGAGGTTCGACTTAAGGAATCTCTGCAGTTGCACATCGATAGTGACTTGTAGGAAAGAAGACAAAAACACGTACAATAAGCAAATTTGAAACATGATTAGGTCATATGAATTTGTTTTAGTAATTGAACATATTTCAAATTAATTAATCTTTACAAAATTATTTATTGGGAAATGCTAACTTGTAATATTTGGGGCTTTATAGCAAGTTATATTATTATATGTTATCTAAATTGGTTTGGATTGGATTTACTTTTATGTATAGTGTGATCCAGAGCAACCGGGTTCTTGTGATGCCGGGTGCAATGGTGGTCTGATGAATAGTGCTTTTGAGTACATTTTAAAATCCGGTGGGGTGATGCGCGAAGAAGACTATCCTTATTCTGCTACTGATCGTGGAGCATGCAAGTTTGACAAGAAAAAAATTGCAGCATCAGTTGCAAATTTCAGCGTTGTTTCACTTGATGAAGACCAGATTGCCGCTAATCTTGTCAAGAACGGTCCTCTTGCTAGTAAGTGCTAAAGTCTTAACAATTTATGTTTTTTTGTTCAATTATAATTATCGAATTCAAATGCAGATATTGATTCTTGTAAATTTCAATGTGCACAAACGCAGTTGCCTTAAATGCAGTATATATGCAAACATACATTGGAGGAGTATCATGCCCATACATATGCTCAAAGAGACTAGATCATGGAGTGTTACTGGTGGGATATGGATCTGGAGCCTATTCGCCTATTAGGATGAAGGAGAAGCCATATTGGATAATTAAAAACTCATGGGGTGAGACTTGGGGAGAAAATGGATACTACAAAATATGCAGGGGACGAAATATATGTGGAGTGGACTCCATGGTTTCTACTGTGGCTGCCGTTCATACTACTACTCAGTAA
Protein sequence

>MS.gene003677.t1

MDHRTLLLFVVLFIFSVSAFSTPDDGEDSIIRQVVDDEGVRLGAEHHFNLFKHKFGKVYSSKDEHDYRFKIFKSNLNRAKKHQLMDPSAVHGVTKFSDLTPREFRQSVLGLRGVGLPKDANAAPILPTDNLPKDFDWREKGAVTAVKNQGSCGSCWSFSTTGALEGAHFLSTGKLVSLSEQQLVDCDHECDPEQPGSCDAGCNGGLMNSAFEYILKSGGVMREEDYPYSATDRGACKFDKKKIAASVANFSVVSLDEDQIAANLVKNGPLAIALNAVYMQTYIGGVSCPYICSKRLDHGVLLVGYGSGAYSPIRMKEKPYWIIKNSWGETWGENGYYKICRGRNICGVDSMVSTVAAVHTTTQ