Alfalfa Gene Editing Database
Nr:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene064873.t1 | XP_003603871.1 | 99.1 | 350 | 3 | 0 | 1 | 350 | 1 | 350 | 2.00E-201 | 711.4 |
Swissprot:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene064873.t1 | Q9LM66 | 73.6 | 356 | 88 | 3 | 1 | 350 | 1 | 356 | 2.3e-150 | 533.1 |
Trembl:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene064873.t1 | G7J6G3 | 99.1 | 350 | 3 | 0 | 1 | 350 | 1 | 350 | 1.4e-201 | 711.4 |
TFs/TRs:
Gene ID | Type | Classification |
---|
Protein Kinases:
Gene ID | Type | Classification |
---|
Network:
Co-expression Network:
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MS.gene051646 | MS.gene064873 | 0.811944 | 5.43E-51 | -1.69E-46 |
PPI:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene064873.t1 | MTR_3g116080 | 99.143 | 350 | 3 | 0 | 1 | 350 | 1 | 350 | 0.0 | 721 |
MS.gene064873.t1 | MTR_1g107550 | 57.100 | 331 | 135 | 5 | 26 | 349 | 27 | 357 | 2.25e-133 | 389 |
MS.gene064873.t1 | MTR_1g018840 | 50.700 | 357 | 164 | 5 | 4 | 348 | 6 | 362 | 2.94e-126 | 370 |
MS.gene064873.t1 | MTR_1g018840 | 50.700 | 357 | 164 | 5 | 4 | 348 | 6 | 362 | 3.84e-126 | 370 |
MS.gene064873.t1 | MTR_3g102220 | 52.260 | 354 | 155 | 7 | 9 | 349 | 11 | 363 | 4.13e-123 | 363 |
MS.gene064873.t1 | MTR_4g104370 | 53.161 | 348 | 151 | 7 | 7 | 349 | 5 | 345 | 1.54e-122 | 357 |
MS.gene064873.t1 | MTR_3g075390 | 51.862 | 349 | 157 | 5 | 5 | 348 | 3 | 345 | 1.82e-122 | 357 |
MS.gene064873.t1 | MTR_4g094918 | 49.128 | 344 | 164 | 4 | 11 | 348 | 4 | 342 | 4.09e-120 | 351 |
MS.gene064873.t1 | MTR_4g079770 | 51.156 | 346 | 154 | 7 | 9 | 347 | 7 | 344 | 1.95e-119 | 348 |
MS.gene064873.t1 | MTR_4g079800 | 53.526 | 312 | 134 | 5 | 43 | 347 | 37 | 344 | 2.82e-119 | 348 |
MS.gene064873.t1 | MTR_4g047610 | 54.921 | 315 | 130 | 6 | 41 | 347 | 32 | 342 | 7.19e-119 | 347 |
MS.gene064873.t1 | MTR_4g079470 | 53.145 | 318 | 138 | 5 | 37 | 347 | 31 | 344 | 1.16e-118 | 347 |
MS.gene064873.t1 | MTR_4g080700 | 55.663 | 309 | 128 | 6 | 43 | 347 | 36 | 339 | 9.07e-117 | 342 |
MS.gene064873.t1 | MTR_5g022560 | 54.984 | 311 | 128 | 6 | 45 | 347 | 37 | 343 | 1.06e-116 | 342 |
MS.gene064873.t1 | MTR_4g107930 | 53.481 | 316 | 135 | 6 | 40 | 347 | 33 | 344 | 7.22e-116 | 340 |
MS.gene064873.t1 | MTR_4g080730 | 55.016 | 309 | 130 | 6 | 43 | 347 | 36 | 339 | 2.96e-114 | 335 |
MS.gene064873.t1 | MTR_3g112430 | 53.074 | 309 | 140 | 3 | 45 | 348 | 34 | 342 | 3.63e-112 | 333 |
MS.gene064873.t1 | MTR_2g075830 | 53.822 | 314 | 132 | 6 | 41 | 347 | 33 | 340 | 8.00e-110 | 324 |
MS.gene064873.t1 | MTR_4g079440 | 51.438 | 313 | 143 | 4 | 36 | 346 | 38 | 343 | 1.79e-109 | 323 |
MS.gene064873.t1 | MTR_2g016180 | 48.837 | 344 | 166 | 6 | 13 | 349 | 17 | 357 | 9.93e-109 | 327 |
MS.gene064873.t1 | MTR_2g016270 | 48.397 | 343 | 154 | 6 | 13 | 349 | 17 | 342 | 5.44e-107 | 322 |
MS.gene064873.t1 | MTR_2g083930 | 49.521 | 313 | 150 | 5 | 42 | 346 | 30 | 342 | 2.06e-105 | 313 |
MS.gene064873.t1 | MTR_4g080360 | 51.133 | 309 | 135 | 6 | 48 | 346 | 38 | 340 | 2.47e-103 | 308 |
MS.gene064873.t1 | MTR_2g090890 | 49.175 | 303 | 149 | 3 | 47 | 348 | 39 | 337 | 1.05e-100 | 301 |
MS.gene064873.t1 | MTR_7g078600 | 47.249 | 309 | 148 | 8 | 43 | 346 | 38 | 336 | 8.88e-94 | 283 |
MS.gene064873.t1 | MTR_7g079230 | 46.753 | 308 | 152 | 7 | 43 | 346 | 35 | 334 | 2.44e-89 | 272 |
MS.gene064873.t1 | MTR_2g015920 | 40.057 | 352 | 194 | 11 | 9 | 348 | 13 | 359 | 1.22e-83 | 258 |
MS.gene064873.t1 | MTR_4g065077 | 40.833 | 360 | 193 | 12 | 1 | 348 | 6 | 357 | 9.52e-79 | 245 |
MS.gene064873.t1 | MTR_2g015850 | 46.758 | 293 | 147 | 7 | 59 | 346 | 74 | 362 | 9.90e-78 | 243 |
MS.gene064873.t1 | MTR_2g015930 | 40.000 | 355 | 191 | 13 | 9 | 348 | 12 | 359 | 2.05e-76 | 239 |
MS.gene064873.t1 | MTR_8g086470 | 38.764 | 356 | 190 | 11 | 1 | 348 | 22 | 357 | 4.15e-72 | 228 |
MS.gene064873.t1 | MTR_4g125300 | 41.042 | 307 | 166 | 9 | 47 | 347 | 51 | 348 | 1.57e-68 | 219 |
MS.gene064873.t1 | MTR_1g023210 | 39.365 | 315 | 166 | 8 | 47 | 344 | 51 | 357 | 1.65e-64 | 209 |
MS.gene064873.t1 | MTR_4g071060 | 38.413 | 315 | 168 | 9 | 47 | 344 | 48 | 353 | 4.48e-63 | 205 |
MS.gene064873.t1 | MTR_2g067900 | 41.667 | 312 | 163 | 10 | 48 | 345 | 17 | 323 | 5.03e-62 | 201 |
MS.gene064873.t1 | MTR_1g094135 | 36.571 | 350 | 192 | 8 | 17 | 344 | 22 | 363 | 1.30e-60 | 199 |
MS.gene064873.t1 | MTR_7g078630 | 43.636 | 275 | 112 | 8 | 41 | 302 | 700 | 944 | 1.74e-58 | 203 |
MS.gene064873.t1 | MTR_2g064475 | 37.209 | 344 | 163 | 13 | 48 | 347 | 17 | 351 | 8.79e-58 | 191 |
MS.gene064873.t1 | MTR_2g067560 | 41.139 | 316 | 153 | 13 | 47 | 339 | 16 | 321 | 8.64e-56 | 196 |
MS.gene064873.t1 | MTR_1g023810 | 41.494 | 241 | 131 | 8 | 110 | 345 | 26 | 261 | 3.52e-54 | 179 |
MS.gene064873.t1 | MTR_4g072790 | 37.183 | 355 | 163 | 13 | 7 | 344 | 6 | 317 | 6.70e-54 | 180 |
MS.gene064873.t1 | MTR_2g066980 | 36.137 | 321 | 147 | 12 | 48 | 347 | 35 | 318 | 5.60e-53 | 177 |
MS.gene064873.t1 | MTR_4g073660 | 35.655 | 359 | 167 | 13 | 7 | 344 | 5 | 320 | 4.75e-51 | 173 |
MS.gene064873.t1 | MTR_4g072340 | 35.294 | 357 | 171 | 13 | 7 | 344 | 6 | 321 | 5.98e-50 | 170 |
MS.gene064873.t1 | MTR_2g016380 | 50.413 | 121 | 56 | 2 | 232 | 349 | 18 | 137 | 2.70e-33 | 124 |
MS.gene064873.t1 | MTR_1g025450 | 39.394 | 198 | 95 | 8 | 113 | 305 | 35 | 212 | 7.06e-33 | 128 |
MS.gene064873.t1 | MTR_7g078580 | 56.122 | 98 | 41 | 2 | 250 | 346 | 4 | 100 | 3.85e-32 | 117 |
MS.gene064873.t1 | MTR_2g015840 | 27.778 | 306 | 157 | 8 | 43 | 347 | 29 | 271 | 1.99e-30 | 117 |
MS.gene064873.t1 | MTR_7g079130 | 54.444 | 90 | 40 | 1 | 258 | 346 | 7 | 96 | 1.63e-27 | 104 |
MS.gene064873.t1 | MTR_7g078310 | 42.222 | 135 | 68 | 4 | 90 | 221 | 117 | 244 | 9.27e-25 | 101 |
MS.gene064873.t1 | MTR_2g047960 | 56.962 | 79 | 34 | 0 | 141 | 219 | 7 | 85 | 7.24e-22 | 89.4 |
MS.gene064873.t1 | MTR_3g029560 | 30.078 | 256 | 144 | 10 | 103 | 346 | 4 | 236 | 3.93e-21 | 91.3 |
MS.gene064873.t1 | MTR_7g111060 | 27.839 | 273 | 162 | 12 | 94 | 339 | 66 | 330 | 1.88e-19 | 88.6 |
MS.gene064873.t1 | MTR_7g111050 | 26.667 | 330 | 204 | 15 | 39 | 339 | 7 | 327 | 3.31e-17 | 82.0 |
MS.gene064873.t1 | MTR_8g087510 | 34.615 | 130 | 79 | 4 | 173 | 300 | 23 | 148 | 3.68e-14 | 70.1 |
MS.gene064873.t1 | MTR_7g079260 | 44.444 | 72 | 23 | 2 | 274 | 344 | 7 | 62 | 1.56e-11 | 60.8 |
MS.gene064873.t1 | MTR_7g111050 | 25.510 | 294 | 187 | 12 | 43 | 309 | 8 | 296 | 9.38e-11 | 62.4 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene064873.t1 | AT1G20850 | 77.439 | 328 | 72 | 2 | 25 | 350 | 29 | 356 | 0.0 | 530 |
MS.gene064873.t1 | AT4G35350 | 69.014 | 355 | 105 | 2 | 1 | 350 | 1 | 355 | 0.0 | 529 |
MS.gene064873.t1 | AT4G35350 | 66.901 | 284 | 89 | 2 | 1 | 279 | 1 | 284 | 1.79e-143 | 407 |
MS.gene064873.t1 | AT5G43060 | 53.333 | 360 | 152 | 5 | 1 | 349 | 1 | 355 | 2.21e-129 | 378 |
MS.gene064873.t1 | AT1G47128 | 52.101 | 357 | 158 | 5 | 1 | 348 | 1 | 353 | 3.38e-129 | 378 |
MS.gene064873.t1 | AT5G50260 | 55.072 | 345 | 144 | 5 | 9 | 348 | 5 | 343 | 4.05e-129 | 374 |
MS.gene064873.t1 | AT3G19390 | 54.070 | 344 | 149 | 6 | 8 | 348 | 9 | 346 | 8.40e-126 | 369 |
MS.gene064873.t1 | AT3G48340 | 51.471 | 340 | 158 | 3 | 15 | 348 | 6 | 344 | 1.24e-118 | 347 |
MS.gene064873.t1 | AT1G09850 | 53.074 | 309 | 144 | 1 | 41 | 348 | 26 | 334 | 6.31e-115 | 340 |
MS.gene064873.t1 | AT4G36880 | 50.155 | 323 | 149 | 4 | 38 | 348 | 40 | 362 | 4.62e-112 | 331 |
MS.gene064873.t1 | AT5G45890 | 48.974 | 341 | 159 | 7 | 17 | 347 | 10 | 345 | 2.39e-111 | 328 |
MS.gene064873.t1 | AT3G19400 | 51.948 | 308 | 142 | 6 | 46 | 348 | 43 | 349 | 1.39e-108 | 322 |
MS.gene064873.t1 | AT4G11310 | 47.175 | 354 | 174 | 6 | 5 | 348 | 3 | 353 | 6.88e-108 | 320 |
MS.gene064873.t1 | AT4G11320 | 46.348 | 356 | 180 | 6 | 1 | 348 | 8 | 360 | 6.81e-107 | 318 |
MS.gene064873.t1 | AT4G11320 | 46.348 | 356 | 180 | 6 | 1 | 348 | 8 | 360 | 6.81e-107 | 318 |
MS.gene064873.t1 | AT1G06260 | 48.538 | 342 | 166 | 5 | 8 | 348 | 11 | 343 | 1.48e-106 | 316 |
MS.gene064873.t1 | AT4G23520 | 44.729 | 351 | 190 | 4 | 1 | 348 | 1 | 350 | 6.72e-106 | 315 |
MS.gene064873.t1 | AT3G48350 | 47.688 | 346 | 169 | 6 | 9 | 348 | 5 | 344 | 7.72e-106 | 315 |
MS.gene064873.t1 | AT3G49340 | 48.232 | 311 | 150 | 6 | 44 | 346 | 32 | 339 | 9.60e-100 | 298 |
MS.gene064873.t1 | AT2G27420 | 47.634 | 317 | 150 | 6 | 44 | 346 | 32 | 346 | 6.34e-98 | 294 |
MS.gene064873.t1 | AT1G29090 | 47.771 | 314 | 153 | 6 | 43 | 347 | 43 | 354 | 5.24e-96 | 289 |
MS.gene064873.t1 | AT2G34080 | 46.474 | 312 | 158 | 5 | 43 | 347 | 35 | 344 | 5.06e-95 | 286 |
MS.gene064873.t1 | AT1G29080 | 42.566 | 343 | 183 | 9 | 15 | 347 | 7 | 345 | 6.04e-92 | 279 |
MS.gene064873.t1 | AT3G43960 | 45.370 | 324 | 166 | 9 | 33 | 348 | 27 | 347 | 4.55e-86 | 265 |
MS.gene064873.t1 | AT1G29110 | 37.647 | 340 | 190 | 6 | 17 | 347 | 7 | 333 | 1.20e-80 | 249 |
MS.gene064873.t1 | AT3G19400 | 51.899 | 237 | 108 | 6 | 46 | 277 | 43 | 278 | 5.94e-77 | 238 |
MS.gene064873.t1 | AT3G48350 | 45.161 | 279 | 142 | 5 | 9 | 282 | 5 | 277 | 7.86e-75 | 233 |
MS.gene064873.t1 | AT5G60360 | 42.671 | 307 | 161 | 9 | 47 | 347 | 59 | 356 | 1.59e-74 | 234 |
MS.gene064873.t1 | AT5G60360 | 42.345 | 307 | 161 | 10 | 47 | 347 | 59 | 355 | 6.43e-72 | 228 |
MS.gene064873.t1 | AT5G60360 | 42.667 | 300 | 157 | 9 | 47 | 340 | 59 | 349 | 9.03e-72 | 228 |
MS.gene064873.t1 | AT4G39090 | 39.940 | 333 | 171 | 9 | 29 | 344 | 37 | 357 | 2.75e-70 | 224 |
MS.gene064873.t1 | AT2G21430 | 39.634 | 328 | 169 | 9 | 34 | 344 | 39 | 354 | 2.82e-69 | 221 |
MS.gene064873.t1 | AT3G45310 | 40.717 | 307 | 167 | 9 | 47 | 347 | 59 | 356 | 2.81e-67 | 216 |
MS.gene064873.t1 | AT3G45310 | 39.474 | 304 | 173 | 7 | 47 | 344 | 59 | 357 | 5.05e-65 | 210 |
MS.gene064873.t1 | AT4G16190 | 39.241 | 316 | 167 | 8 | 47 | 344 | 55 | 363 | 3.52e-61 | 201 |
MS.gene064873.t1 | AT3G54940 | 35.655 | 359 | 199 | 9 | 10 | 344 | 9 | 359 | 2.39e-60 | 198 |
MS.gene064873.t1 | AT3G54940 | 34.201 | 269 | 162 | 4 | 10 | 264 | 9 | 276 | 9.51e-46 | 157 |
MS.gene064873.t1 | AT4G01610 | 26.562 | 320 | 193 | 15 | 59 | 349 | 36 | 342 | 1.21e-16 | 80.5 |
MS.gene064873.t1 | AT4G01610 | 26.250 | 320 | 194 | 15 | 59 | 349 | 36 | 342 | 1.77e-15 | 77.0 |
Find 88 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
ACTCAGGTTCGAGGTCTTTA+AGG | 0.183217 | 3.3:+94679926 | MS.gene064873:CDS |
ACTGATGTTGTGACTATTAA+TGG | 0.222069 | 3.3:+94680991 | MS.gene064873:CDS |
TCAAATTCCCTGTCACAATT+TGG | 0.244699 | 3.3:-94680693 | None:intergenic |
GCAGGTCAGGACAAGTGTTT+TGG | 0.258810 | 3.3:-94679748 | None:intergenic |
GGAAGATTATCCTTACATTA+TGG | 0.312016 | 3.3:+94680834 | MS.gene064873:CDS |
ATCTCTGCCTGAAGCCTCTA+TGG | 0.319266 | 3.3:-94681085 | None:intergenic |
ACTTGTTCTTGAACTCTTGA+TGG | 0.328420 | 3.3:-94680028 | None:intergenic |
ATATGGTACATCAAAGGGTT+TGG | 0.369364 | 3.3:+94681275 | MS.gene064873:CDS |
TTATTTCTATCTTTAGCATT+TGG | 0.374681 | 3.3:+94679780 | MS.gene064873:CDS |
CATTTGTAGGGTGTGTTTGA+TGG | 0.384261 | 3.3:+94681210 | MS.gene064873:intron |
AAGCACTTGCGAGATGAAAA+AGG | 0.391386 | 3.3:+94680861 | MS.gene064873:CDS |
GAGTACTTGCAGGTAGTTGC+TGG | 0.397924 | 3.3:+94680635 | MS.gene064873:intron |
AACATACTCCACTATAGAAC+TGG | 0.404978 | 3.3:-94681110 | None:intergenic |
CATTGACCTTGATTCTTGAC+CGG | 0.416938 | 3.3:-94680167 | None:intergenic |
ACATTATAGTGAAGAATTCA+TGG | 0.418831 | 3.3:+94681301 | MS.gene064873:CDS |
TTTCTCCAATCAACTGACTT+AGG | 0.421540 | 3.3:-94680128 | None:intergenic |
TCAACTGTTGCCGCGGTTGA+AGG | 0.428053 | 3.3:+94680664 | MS.gene064873:CDS |
ATAAGCATAAGGAGCAGGTC+AGG | 0.428907 | 3.3:-94679761 | None:intergenic |
GGAGCAAAGTGGGGTGAGAA+AGG | 0.429527 | 3.3:+94681324 | MS.gene064873:CDS |
ATTTGCCTAAGTCAGTTGAT+TGG | 0.430861 | 3.3:+94680123 | MS.gene064873:CDS |
CTTACAACAATGGTTGCAAT+GGG | 0.434061 | 3.3:+94680755 | MS.gene064873:CDS |
GGGGTGAGAAAGGGTTTATA+AGG | 0.436382 | 3.3:+94681334 | MS.gene064873:CDS |
GGTGTGTCAGCCGTTGGATA+TGG | 0.454497 | 3.3:+94681258 | MS.gene064873:CDS |
AGTTCAAGAACAAGTATCTT+GGG | 0.458742 | 3.3:+94680036 | MS.gene064873:CDS |
GAACAAGTATCTTGGGCTAA+AGG | 0.462245 | 3.3:+94680043 | MS.gene064873:CDS |
GAGTTCAAGAACAAGTATCT+TGG | 0.466462 | 3.3:+94680035 | MS.gene064873:CDS |
AGAGATTTCCAGTTCTATAG+TGG | 0.466790 | 3.3:+94681102 | MS.gene064873:CDS |
GGAATAAACCAAATTGTGAC+AGG | 0.467218 | 3.3:+94680685 | MS.gene064873:CDS |
GGAAAGTCTGAAGGGATTTG+TGG | 0.476760 | 3.3:+94681372 | MS.gene064873:CDS |
TCATTGAACTCTTCGAATCA+TGG | 0.479297 | 3.3:+94679859 | MS.gene064873:CDS |
GAATTCATGGGGAGCAAAGT+GGG | 0.481502 | 3.3:+94681314 | MS.gene064873:CDS |
TCCATGGACTTCAAGTCCTC+TGG | 0.486674 | 3.3:-94679831 | None:intergenic |
TAATGAACAAAGCTTATTGA+AGG | 0.491336 | 3.3:+94681035 | MS.gene064873:CDS |
GAGCAAAGTGGGGTGAGAAA+GGG | 0.494121 | 3.3:+94681325 | MS.gene064873:CDS |
ATCTAAAGCACATTGATGAT+AGG | 0.494574 | 3.3:+94679952 | MS.gene064873:CDS |
GTGCTTTCCTCCATAATGTA+AGG | 0.494914 | 3.3:-94680844 | None:intergenic |
TAGAAATAAGCATAAGGAGC+AGG | 0.496949 | 3.3:-94679766 | None:intergenic |
ACAAGGTTGTCAGCAACTAC+TGG | 0.500291 | 3.3:+94679976 | MS.gene064873:CDS |
GTTGTCAGCAACTACTGGCT+TGG | 0.503373 | 3.3:+94679981 | MS.gene064873:CDS |
ATTGACCTTGATTCTTGACC+GGG | 0.509041 | 3.3:-94680166 | None:intergenic |
TCAGTTGATTGGAGAAAGAA+AGG | 0.509647 | 3.3:+94680134 | MS.gene064873:CDS |
TAAAGATAGAAATAAGCATA+AGG | 0.512917 | 3.3:-94679772 | None:intergenic |
GTTGGATATGGTACATCAAA+GGG | 0.516544 | 3.3:+94681270 | MS.gene064873:CDS |
CTATGGCCACACTGAGTGGT+TGG | 0.516563 | 3.3:-94681068 | None:intergenic |
CGTTGGATATGGTACATCAA+AGG | 0.524173 | 3.3:+94681269 | MS.gene064873:CDS |
ATAAGGATGAAGAGAAACAT+TGG | 0.526478 | 3.3:+94681351 | MS.gene064873:CDS |
AGAAACATTGGAAAGTCTGA+AGG | 0.526996 | 3.3:+94681363 | MS.gene064873:CDS |
CATTATAGTGAAGAATTCAT+GGG | 0.532840 | 3.3:+94681302 | MS.gene064873:CDS |
ACTTACAACAATGGTTGCAA+TGG | 0.533682 | 3.3:+94680754 | MS.gene064873:CDS |
GGACGTGATTTCTCCATCGT+GGG | 0.535021 | 3.3:+94679801 | MS.gene064873:CDS |
TACAACAATGGTTGCAATGG+GGG | 0.543003 | 3.3:+94680757 | MS.gene064873:CDS |
ACCACTCAGTGTGGCCATAG+AGG | 0.545434 | 3.3:+94681071 | MS.gene064873:CDS |
AAAGAAAGGTGCTGTAACCC+CGG | 0.549002 | 3.3:+94680148 | MS.gene064873:CDS |
TAAAGGTTGACTTGTCTCAA+AGG | 0.550091 | 3.3:+94680060 | MS.gene064873:CDS |
GATCATGGTGTGTCAGCCGT+TGG | 0.556769 | 3.3:+94681252 | MS.gene064873:CDS |
GTAACCCCGGTCAAGAATCA+AGG | 0.557737 | 3.3:+94680161 | MS.gene064873:CDS |
AAACGGTGGACTACATAAAG+AGG | 0.559559 | 3.3:+94680813 | MS.gene064873:CDS |
AGTGTGGCCATAGAGGCTTC+AGG | 0.567247 | 3.3:+94681078 | MS.gene064873:CDS |
GAGTTCAATGAGCTTATCCA+TGG | 0.571450 | 3.3:-94679847 | None:intergenic |
ACCAGAGGACTTGAAGTCCA+TGG | 0.576800 | 3.3:+94679830 | MS.gene064873:CDS |
TGGACGTGATTTCTCCATCG+TGG | 0.576917 | 3.3:+94679800 | MS.gene064873:CDS |
TGCGGAAGTGAACTAGATCA+TGG | 0.579661 | 3.3:+94681237 | MS.gene064873:CDS |
CCATTGAAGAAAAGCTACTC+AGG | 0.581738 | 3.3:+94679910 | MS.gene064873:CDS |
AGAAAAGCTACTCAGGTTCG+AGG | 0.582875 | 3.3:+94679917 | MS.gene064873:CDS |
AAGGAGAGAGTCCTCAGAAG+AGG | 0.591057 | 3.3:+94680079 | MS.gene064873:CDS |
AGAATTCATGGGGAGCAAAG+TGG | 0.591803 | 3.3:+94681313 | MS.gene064873:CDS |
GGCCACACTGAGTGGTTGGT+TGG | 0.597846 | 3.3:-94681064 | None:intergenic |
ATAAGTGAATTCCTCTTCTG+AGG | 0.598495 | 3.3:-94680090 | None:intergenic |
AGTACTTGCAGGTAGTTGCT+GGG | 0.604719 | 3.3:+94680636 | MS.gene064873:intron |
GCCTCTATGGCCACACTGAG+TGG | 0.610194 | 3.3:-94681072 | None:intergenic |
TTGGTTTATTCCTTCAACCG+CGG | 0.610274 | 3.3:-94680674 | None:intergenic |
AGATTATCCTTACATTATGG+AGG | 0.615877 | 3.3:+94680837 | MS.gene064873:CDS |
GAAACATTGGAAAGTCTGAA+GGG | 0.619388 | 3.3:+94681364 | MS.gene064873:CDS |
GATTTGTGGACTCTACAAGA+TGG | 0.625513 | 3.3:+94681386 | MS.gene064873:CDS |
CTTTGATGTACCATATCCAA+CGG | 0.626421 | 3.3:-94681268 | None:intergenic |
TGCGACACAACTTACAACAA+TGG | 0.635197 | 3.3:+94680745 | MS.gene064873:CDS |
GAATCATGGATGTCGAGACA+CGG | 0.654814 | 3.3:+94679873 | MS.gene064873:CDS |
CTCTGGTGAATAGCCCACGA+TGG | 0.663678 | 3.3:-94679814 | None:intergenic |
ATTATAGTGAAGAATTCATG+GGG | 0.665269 | 3.3:+94681303 | MS.gene064873:CDS |
GCACATTGATGATAGGAACA+AGG | 0.668624 | 3.3:+94679959 | MS.gene064873:CDS |
AATTCATGGGGAGCAAAGTG+GGG | 0.670875 | 3.3:+94681315 | MS.gene064873:CDS |
TGCCAACCAACCACTCAGTG+TGG | 0.678227 | 3.3:+94681062 | MS.gene064873:CDS |
CATCGTGGGCTATTCACCAG+AGG | 0.679810 | 3.3:+94679815 | MS.gene064873:CDS |
GTCAAGAATCAAGGTCAATG+TGG | 0.700857 | 3.3:+94680170 | MS.gene064873:CDS |
GAATAAACCAAATTGTGACA+GGG | 0.702620 | 3.3:+94680686 | MS.gene064873:CDS |
TTACAACAATGGTTGCAATG+GGG | 0.703567 | 3.3:+94680756 | MS.gene064873:CDS |
TTGACCTTGATTCTTGACCG+GGG | 0.717921 | 3.3:-94680165 | None:intergenic |
GGTGTGTTTGATGGACATTG+CGG | 0.757484 | 3.3:+94681219 | MS.gene064873:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
!!! | TCAAGTATTTTTTTTTTTTT+TGG | - | chr3.3:94680364-94680383 | None:intergenic | 10.0% |
!! | ATAAGTAACATAACTTTCAT+TGG | + | chr3.3:94680949-94680968 | MS.gene064873:intron | 20.0% |
!! | ATTATTTAACTACCATTTGT+AGG | + | chr3.3:94681197-94681216 | MS.gene064873:intron | 20.0% |
!! | CTATATATACTATTGGATAA+CGG | + | chr3.3:94680543-94680562 | MS.gene064873:intron | 20.0% |
!! | TAAAGATAGAAATAAGCATA+AGG | - | chr3.3:94679775-94679794 | None:intergenic | 20.0% |
!! | TTATTTAACTACCATTTGTA+GGG | + | chr3.3:94681198-94681217 | MS.gene064873:intron | 20.0% |
!!! | TTATTTCTATCTTTAGCATT+TGG | + | chr3.3:94679780-94679799 | MS.gene064873:CDS | 20.0% |
! | AACGAAAAATTTGCATGATT+AGG | - | chr3.3:94680480-94680499 | None:intergenic | 25.0% |
! | ACATTATAGTGAAGAATTCA+TGG | + | chr3.3:94681301-94681320 | MS.gene064873:CDS | 25.0% |
! | ATCATTGAATTGATATCAGA+CGG | - | chr3.3:94680400-94680419 | None:intergenic | 25.0% |
! | ATTATAGTGAAGAATTCATG+GGG | + | chr3.3:94681303-94681322 | MS.gene064873:CDS | 25.0% |
! | CATTATAGTGAAGAATTCAT+GGG | + | chr3.3:94681302-94681321 | MS.gene064873:CDS | 25.0% |
! | GTTCAGTCTATATATACTAT+TGG | + | chr3.3:94680536-94680555 | MS.gene064873:intron | 25.0% |
! | TAATGAACAAAGCTTATTGA+AGG | + | chr3.3:94681035-94681054 | MS.gene064873:CDS | 25.0% |
! | TTCTCCTTCATTGTAAAAAA+CGG | + | chr3.3:94680796-94680815 | MS.gene064873:CDS | 25.0% |
!!! | AAGCTTTGTTCATTATTTTG+TGG | - | chr3.3:94681030-94681049 | None:intergenic | 25.0% |
ACTGATGTTGTGACTATTAA+TGG | + | chr3.3:94680991-94681010 | MS.gene064873:CDS | 30.0% | |
AGATTATCCTTACATTATGG+AGG | + | chr3.3:94680837-94680856 | MS.gene064873:CDS | 30.0% | |
AGTTCAAGAACAAGTATCTT+GGG | + | chr3.3:94680036-94680055 | MS.gene064873:CDS | 30.0% | |
ATAAGGATGAAGAGAAACAT+TGG | + | chr3.3:94681351-94681370 | MS.gene064873:CDS | 30.0% | |
GAATAAACCAAATTGTGACA+GGG | + | chr3.3:94680686-94680705 | MS.gene064873:CDS | 30.0% | |
GGAAGATTATCCTTACATTA+TGG | + | chr3.3:94680834-94680853 | MS.gene064873:CDS | 30.0% | |
TAGGCATGTTTACATATAGT+AGG | - | chr3.3:94680461-94680480 | None:intergenic | 30.0% | |
TGGGTGAATCTAATCTTAAA+TGG | + | chr3.3:94681160-94681179 | MS.gene064873:intron | 30.0% | |
! | ATCTAAAGCACATTGATGAT+AGG | + | chr3.3:94679952-94679971 | MS.gene064873:CDS | 30.0% |
AACATACTCCACTATAGAAC+TGG | - | chr3.3:94681113-94681132 | None:intergenic | 35.0% | |
ACTTACAACAATGGTTGCAA+TGG | + | chr3.3:94680754-94680773 | MS.gene064873:CDS | 35.0% | |
ACTTGTTCTTGAACTCTTGA+TGG | - | chr3.3:94680031-94680050 | None:intergenic | 35.0% | |
AGAAACATTGGAAAGTCTGA+AGG | + | chr3.3:94681363-94681382 | MS.gene064873:CDS | 35.0% | |
AGAGATTTCCAGTTCTATAG+TGG | + | chr3.3:94681102-94681121 | MS.gene064873:CDS | 35.0% | |
ATAAGTGAATTCCTCTTCTG+AGG | - | chr3.3:94680093-94680112 | None:intergenic | 35.0% | |
ATATGGTACATCAAAGGGTT+TGG | + | chr3.3:94681275-94681294 | MS.gene064873:CDS | 35.0% | |
ATTTGCCTAAGTCAGTTGAT+TGG | + | chr3.3:94680123-94680142 | MS.gene064873:CDS | 35.0% | |
CTTACAACAATGGTTGCAAT+GGG | + | chr3.3:94680755-94680774 | MS.gene064873:CDS | 35.0% | |
CTTTGATGTACCATATCCAA+CGG | - | chr3.3:94681271-94681290 | None:intergenic | 35.0% | |
GAAACATTGGAAAGTCTGAA+GGG | + | chr3.3:94681364-94681383 | MS.gene064873:CDS | 35.0% | |
GAGTTCAAGAACAAGTATCT+TGG | + | chr3.3:94680035-94680054 | MS.gene064873:CDS | 35.0% | |
GGAATAAACCAAATTGTGAC+AGG | + | chr3.3:94680685-94680704 | MS.gene064873:CDS | 35.0% | |
GTTGGATATGGTACATCAAA+GGG | + | chr3.3:94681270-94681289 | MS.gene064873:CDS | 35.0% | |
TAGAAATAAGCATAAGGAGC+AGG | - | chr3.3:94679769-94679788 | None:intergenic | 35.0% | |
TCAAATTCCCTGTCACAATT+TGG | - | chr3.3:94680696-94680715 | None:intergenic | 35.0% | |
TCAGTTGATTGGAGAAAGAA+AGG | + | chr3.3:94680134-94680153 | MS.gene064873:CDS | 35.0% | |
TCATTGAACTCTTCGAATCA+TGG | + | chr3.3:94679859-94679878 | MS.gene064873:CDS | 35.0% | |
TCCTTCATTGTAAAAAACGG+TGG | + | chr3.3:94680799-94680818 | MS.gene064873:CDS | 35.0% | |
TTACAACAATGGTTGCAATG+GGG | + | chr3.3:94680756-94680775 | MS.gene064873:CDS | 35.0% | |
! | TTTCTCCAATCAACTGACTT+AGG | - | chr3.3:94680131-94680150 | None:intergenic | 35.0% |
!! | TAAAGGTTGACTTGTCTCAA+AGG | + | chr3.3:94680060-94680079 | MS.gene064873:CDS | 35.0% |
!! | TTTCATTGGTTTGTTTCACC+AGG | + | chr3.3:94680963-94680982 | MS.gene064873:intron | 35.0% |
!!! | TCCACCGTTTTTTACAATGA+AGG | - | chr3.3:94680803-94680822 | None:intergenic | 35.0% |
AAGCACTTGCGAGATGAAAA+AGG | + | chr3.3:94680861-94680880 | MS.gene064873:CDS | 40.0% | |
AGCTTGCAAGCATATTGACA+TGG | + | chr3.3:94680600-94680619 | MS.gene064873:intron | 40.0% | |
ATTGACCTTGATTCTTGACC+GGG | - | chr3.3:94680169-94680188 | None:intergenic | 40.0% | |
CATCAAACACACCCTACAAA+TGG | - | chr3.3:94681212-94681231 | None:intergenic | 40.0% | |
CATTGACCTTGATTCTTGAC+CGG | - | chr3.3:94680170-94680189 | None:intergenic | 40.0% | |
CATTTGTAGGGTGTGTTTGA+TGG | + | chr3.3:94681210-94681229 | MS.gene064873:intron | 40.0% | |
CCATTGAAGAAAAGCTACTC+AGG | + | chr3.3:94679910-94679929 | MS.gene064873:CDS | 40.0% | |
CGTTGGATATGGTACATCAA+AGG | + | chr3.3:94681269-94681288 | MS.gene064873:CDS | 40.0% | |
GAACAAGTATCTTGGGCTAA+AGG | + | chr3.3:94680043-94680062 | MS.gene064873:CDS | 40.0% | |
GAGTTCAATGAGCTTATCCA+TGG | - | chr3.3:94679850-94679869 | None:intergenic | 40.0% | |
GATTTGTGGACTCTACAAGA+TGG | + | chr3.3:94681386-94681405 | MS.gene064873:CDS | 40.0% | |
GTCAAGAATCAAGGTCAATG+TGG | + | chr3.3:94680170-94680189 | MS.gene064873:CDS | 40.0% | |
GTGCTTTCCTCCATAATGTA+AGG | - | chr3.3:94680847-94680866 | None:intergenic | 40.0% | |
TACAACAATGGTTGCAATGG+GGG | + | chr3.3:94680757-94680776 | MS.gene064873:CDS | 40.0% | |
TCACAACATCAGTTACTTCC+TGG | - | chr3.3:94680984-94681003 | None:intergenic | 40.0% | |
TGCGACACAACTTACAACAA+TGG | + | chr3.3:94680745-94680764 | MS.gene064873:CDS | 40.0% | |
TTGCAAGCATATTGACATGG+TGG | + | chr3.3:94680603-94680622 | MS.gene064873:intron | 40.0% | |
TTGGTTTATTCCTTCAACCG+CGG | - | chr3.3:94680677-94680696 | None:intergenic | 40.0% | |
! | CCTGAGTAGCTTTTCTTCAA+TGG | - | chr3.3:94679913-94679932 | None:intergenic | 40.0% |
! | GCACATTGATGATAGGAACA+AGG | + | chr3.3:94679959-94679978 | MS.gene064873:CDS | 40.0% |
!! | AAACGGTGGACTACATAAAG+AGG | + | chr3.3:94680813-94680832 | MS.gene064873:CDS | 40.0% |
AATTCATGGGGAGCAAAGTG+GGG | + | chr3.3:94681315-94681334 | MS.gene064873:CDS | 45.0% | |
ACAAGGTTGTCAGCAACTAC+TGG | + | chr3.3:94679976-94679995 | MS.gene064873:CDS | 45.0% | |
ACTCAGGTTCGAGGTCTTTA+AGG | + | chr3.3:94679926-94679945 | MS.gene064873:CDS | 45.0% | |
AGAAAAGCTACTCAGGTTCG+AGG | + | chr3.3:94679917-94679936 | MS.gene064873:CDS | 45.0% | |
AGAATTCATGGGGAGCAAAG+TGG | + | chr3.3:94681313-94681332 | MS.gene064873:CDS | 45.0% | |
AGTACTTGCAGGTAGTTGCT+GGG | + | chr3.3:94680636-94680655 | MS.gene064873:intron | 45.0% | |
ATAAGCATAAGGAGCAGGTC+AGG | - | chr3.3:94679764-94679783 | None:intergenic | 45.0% | |
GAATCATGGATGTCGAGACA+CGG | + | chr3.3:94679873-94679892 | MS.gene064873:CDS | 45.0% | |
GAATTCATGGGGAGCAAAGT+GGG | + | chr3.3:94681314-94681333 | MS.gene064873:CDS | 45.0% | |
GGAAAGTCTGAAGGGATTTG+TGG | + | chr3.3:94681372-94681391 | MS.gene064873:CDS | 45.0% | |
GGGGTGAGAAAGGGTTTATA+AGG | + | chr3.3:94681334-94681353 | MS.gene064873:CDS | 45.0% | |
GTATGAACACGAGTACTTGC+AGG | + | chr3.3:94680625-94680644 | MS.gene064873:intron | 45.0% | |
TGCGGAAGTGAACTAGATCA+TGG | + | chr3.3:94681237-94681256 | MS.gene064873:CDS | 45.0% | |
TTGACCTTGATTCTTGACCG+GGG | - | chr3.3:94680168-94680187 | None:intergenic | 45.0% | |
! | AGGTCAGGACAAGTGTTTTG+GGG | - | chr3.3:94679749-94679768 | None:intergenic | 45.0% |
! | CAGGTCAGGACAAGTGTTTT+GGG | - | chr3.3:94679750-94679769 | None:intergenic | 45.0% |
! | GGTGTGTTTGATGGACATTG+CGG | + | chr3.3:94681219-94681238 | MS.gene064873:CDS | 45.0% |
!! | AAAGAAAGGTGCTGTAACCC+CGG | + | chr3.3:94680148-94680167 | MS.gene064873:CDS | 45.0% |
!!! | TAATTATTGTTTTTTTTAAT+GGG | + | chr3.3:94681141-94681160 | MS.gene064873:intron | 5.0% |
!!! | TTAATTATTGTTTTTTTTAA+TGG | + | chr3.3:94681140-94681159 | MS.gene064873:intron | 5.0% |
AAGGAGAGAGTCCTCAGAAG+AGG | + | chr3.3:94680079-94680098 | MS.gene064873:CDS | 50.0% | |
ACCAGAGGACTTGAAGTCCA+TGG | + | chr3.3:94679830-94679849 | MS.gene064873:CDS | 50.0% | |
ATCTCTGCCTGAAGCCTCTA+TGG | - | chr3.3:94681088-94681107 | None:intergenic | 50.0% | |
CATCCACATAGTCGATGCAC+TGG | + | chr3.3:94680248-94680267 | MS.gene064873:intron | 50.0% | |
GAGCAAAGTGGGGTGAGAAA+GGG | + | chr3.3:94681325-94681344 | MS.gene064873:CDS | 50.0% | |
GAGTACTTGCAGGTAGTTGC+TGG | + | chr3.3:94680635-94680654 | MS.gene064873:intron | 50.0% | |
GGACGTGATTTCTCCATCGT+GGG | + | chr3.3:94679801-94679820 | MS.gene064873:CDS | 50.0% | |
GTAACCCCGGTCAAGAATCA+AGG | + | chr3.3:94680161-94680180 | MS.gene064873:CDS | 50.0% | |
TCCATGGACTTCAAGTCCTC+TGG | - | chr3.3:94679834-94679853 | None:intergenic | 50.0% | |
TGGACGTGATTTCTCCATCG+TGG | + | chr3.3:94679800-94679819 | MS.gene064873:CDS | 50.0% | |
TGGTTGCAATGGGGGTCTAA+TGG | + | chr3.3:94680765-94680784 | MS.gene064873:CDS | 50.0% | |
! | GCAGGTCAGGACAAGTGTTT+TGG | - | chr3.3:94679751-94679770 | None:intergenic | 50.0% |
! | GGCATTTTCAACTGTTGCCG+CGG | + | chr3.3:94680657-94680676 | MS.gene064873:CDS | 50.0% |
! | GTTGTCAGCAACTACTGGCT+TGG | + | chr3.3:94679981-94680000 | MS.gene064873:CDS | 50.0% |
!! | GAACCAGTGCATCGACTATG+TGG | - | chr3.3:94680254-94680273 | None:intergenic | 50.0% |
!! | GGTCAGGACAAGTGTTTTGG+GGG | - | chr3.3:94679748-94679767 | None:intergenic | 50.0% |
!! | GTGCATCGACTATGTGGATG+CGG | - | chr3.3:94680248-94680267 | None:intergenic | 50.0% |
!! | TGCATCGACTATGTGGATGC+GGG | - | chr3.3:94680247-94680266 | None:intergenic | 50.0% |
ACCACTCAGTGTGGCCATAG+AGG | + | chr3.3:94681071-94681090 | MS.gene064873:CDS | 55.0% | |
AGTGTGGCCATAGAGGCTTC+AGG | + | chr3.3:94681078-94681097 | MS.gene064873:CDS | 55.0% | |
CTATGGCCACACTGAGTGGT+TGG | - | chr3.3:94681071-94681090 | None:intergenic | 55.0% | |
CTCTGGTGAATAGCCCACGA+TGG | - | chr3.3:94679817-94679836 | None:intergenic | 55.0% | |
GATCATGGTGTGTCAGCCGT+TGG | + | chr3.3:94681252-94681271 | MS.gene064873:CDS | 55.0% | |
GGAGCAAAGTGGGGTGAGAA+AGG | + | chr3.3:94681324-94681343 | MS.gene064873:CDS | 55.0% | |
GGTGTGTCAGCCGTTGGATA+TGG | + | chr3.3:94681258-94681277 | MS.gene064873:CDS | 55.0% | |
TCAACTGTTGCCGCGGTTGA+AGG | + | chr3.3:94680664-94680683 | MS.gene064873:CDS | 55.0% | |
TGCCAACCAACCACTCAGTG+TGG | + | chr3.3:94681062-94681081 | MS.gene064873:CDS | 55.0% | |
! | CATCGTGGGCTATTCACCAG+AGG | + | chr3.3:94679815-94679834 | MS.gene064873:CDS | 55.0% |
!! | GCATCGACTATGTGGATGCG+GGG | - | chr3.3:94680246-94680265 | None:intergenic | 55.0% |
GCCTCTATGGCCACACTGAG+TGG | - | chr3.3:94681075-94681094 | None:intergenic | 60.0% | |
GGCCACACTGAGTGGTTGGT+TGG | - | chr3.3:94681067-94681086 | None:intergenic | 60.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
chr3.3 | gene | 94679732 | 94681434 | 94679732 | ID=MS.gene064873 |
chr3.3 | mRNA | 94679732 | 94681434 | 94679732 | ID=MS.gene064873.t1;Parent=MS.gene064873 |
chr3.3 | exon | 94679732 | 94680191 | 94679732 | ID=MS.gene064873.t1.exon1;Parent=MS.gene064873.t1 |
chr3.3 | CDS | 94679732 | 94680191 | 94679732 | ID=cds.MS.gene064873.t1;Parent=MS.gene064873.t1 |
chr3.3 | exon | 94680647 | 94680882 | 94680647 | ID=MS.gene064873.t1.exon2;Parent=MS.gene064873.t1 |
chr3.3 | CDS | 94680647 | 94680882 | 94680647 | ID=cds.MS.gene064873.t1;Parent=MS.gene064873.t1 |
chr3.3 | exon | 94680985 | 94681125 | 94680985 | ID=MS.gene064873.t1.exon3;Parent=MS.gene064873.t1 |
chr3.3 | CDS | 94680985 | 94681125 | 94680985 | ID=cds.MS.gene064873.t1;Parent=MS.gene064873.t1 |
chr3.3 | exon | 94681219 | 94681434 | 94681219 | ID=MS.gene064873.t1.exon4;Parent=MS.gene064873.t1 |
chr3.3 | CDS | 94681219 | 94681434 | 94681219 | ID=cds.MS.gene064873.t1;Parent=MS.gene064873.t1 |
Gene Sequence |
Protein sequence |