AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66833

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66833.t1 MTR_3g116080 99.143 350 3 0 1 350 1 350 0.0 721
MS.gene66833.t1 MTR_1g107550 57.100 331 135 5 26 349 27 357 2.25e-133 389
MS.gene66833.t1 MTR_1g018840 50.700 357 164 5 4 348 6 362 2.94e-126 370
MS.gene66833.t1 MTR_1g018840 50.700 357 164 5 4 348 6 362 3.84e-126 370
MS.gene66833.t1 MTR_3g102220 52.260 354 155 7 9 349 11 363 4.13e-123 363
MS.gene66833.t1 MTR_4g104370 53.161 348 151 7 7 349 5 345 1.54e-122 357
MS.gene66833.t1 MTR_3g075390 51.862 349 157 5 5 348 3 345 1.82e-122 357
MS.gene66833.t1 MTR_4g094918 49.128 344 164 4 11 348 4 342 4.09e-120 351
MS.gene66833.t1 MTR_4g079770 51.156 346 154 7 9 347 7 344 1.95e-119 348
MS.gene66833.t1 MTR_4g079800 53.526 312 134 5 43 347 37 344 2.82e-119 348
MS.gene66833.t1 MTR_4g047610 54.921 315 130 6 41 347 32 342 7.19e-119 347
MS.gene66833.t1 MTR_4g079470 53.145 318 138 5 37 347 31 344 1.16e-118 347
MS.gene66833.t1 MTR_4g080700 55.663 309 128 6 43 347 36 339 9.07e-117 342
MS.gene66833.t1 MTR_5g022560 54.984 311 128 6 45 347 37 343 1.06e-116 342
MS.gene66833.t1 MTR_4g107930 53.481 316 135 6 40 347 33 344 7.22e-116 340
MS.gene66833.t1 MTR_4g080730 55.016 309 130 6 43 347 36 339 2.96e-114 335
MS.gene66833.t1 MTR_3g112430 53.074 309 140 3 45 348 34 342 3.63e-112 333
MS.gene66833.t1 MTR_2g075830 53.822 314 132 6 41 347 33 340 8.00e-110 324
MS.gene66833.t1 MTR_4g079440 51.438 313 143 4 36 346 38 343 1.79e-109 323
MS.gene66833.t1 MTR_2g016180 48.837 344 166 6 13 349 17 357 9.93e-109 327
MS.gene66833.t1 MTR_2g016270 48.397 343 154 6 13 349 17 342 5.44e-107 322
MS.gene66833.t1 MTR_2g083930 49.521 313 150 5 42 346 30 342 2.06e-105 313
MS.gene66833.t1 MTR_4g080360 51.133 309 135 6 48 346 38 340 2.47e-103 308
MS.gene66833.t1 MTR_2g090890 49.175 303 149 3 47 348 39 337 1.05e-100 301
MS.gene66833.t1 MTR_7g078600 47.249 309 148 8 43 346 38 336 8.88e-94 283
MS.gene66833.t1 MTR_7g079230 46.753 308 152 7 43 346 35 334 2.44e-89 272
MS.gene66833.t1 MTR_2g015920 40.057 352 194 11 9 348 13 359 1.22e-83 258
MS.gene66833.t1 MTR_4g065077 40.833 360 193 12 1 348 6 357 9.52e-79 245
MS.gene66833.t1 MTR_2g015850 46.758 293 147 7 59 346 74 362 9.90e-78 243
MS.gene66833.t1 MTR_2g015930 40.000 355 191 13 9 348 12 359 2.05e-76 239
MS.gene66833.t1 MTR_8g086470 38.764 356 190 11 1 348 22 357 4.15e-72 228
MS.gene66833.t1 MTR_4g125300 41.042 307 166 9 47 347 51 348 1.57e-68 219
MS.gene66833.t1 MTR_1g023210 39.365 315 166 8 47 344 51 357 1.65e-64 209
MS.gene66833.t1 MTR_4g071060 38.413 315 168 9 47 344 48 353 4.48e-63 205
MS.gene66833.t1 MTR_2g067900 41.667 312 163 10 48 345 17 323 5.03e-62 201
MS.gene66833.t1 MTR_1g094135 36.571 350 192 8 17 344 22 363 1.30e-60 199
MS.gene66833.t1 MTR_7g078630 43.636 275 112 8 41 302 700 944 1.74e-58 203
MS.gene66833.t1 MTR_2g064475 37.209 344 163 13 48 347 17 351 8.79e-58 191
MS.gene66833.t1 MTR_2g067560 41.139 316 153 13 47 339 16 321 8.64e-56 196
MS.gene66833.t1 MTR_1g023810 41.494 241 131 8 110 345 26 261 3.52e-54 179
MS.gene66833.t1 MTR_4g072790 37.183 355 163 13 7 344 6 317 6.70e-54 180
MS.gene66833.t1 MTR_2g066980 36.137 321 147 12 48 347 35 318 5.60e-53 177
MS.gene66833.t1 MTR_4g073660 35.655 359 167 13 7 344 5 320 4.75e-51 173
MS.gene66833.t1 MTR_4g072340 35.294 357 171 13 7 344 6 321 5.98e-50 170
MS.gene66833.t1 MTR_2g016380 50.413 121 56 2 232 349 18 137 2.70e-33 124
MS.gene66833.t1 MTR_1g025450 39.394 198 95 8 113 305 35 212 7.06e-33 128
MS.gene66833.t1 MTR_7g078580 56.122 98 41 2 250 346 4 100 3.85e-32 117
MS.gene66833.t1 MTR_2g015840 27.778 306 157 8 43 347 29 271 1.99e-30 117
MS.gene66833.t1 MTR_7g079130 54.444 90 40 1 258 346 7 96 1.63e-27 104
MS.gene66833.t1 MTR_7g078310 42.222 135 68 4 90 221 117 244 9.27e-25 101
MS.gene66833.t1 MTR_2g047960 56.962 79 34 0 141 219 7 85 7.24e-22 89.4
MS.gene66833.t1 MTR_3g029560 30.078 256 144 10 103 346 4 236 3.93e-21 91.3
MS.gene66833.t1 MTR_7g111060 27.839 273 162 12 94 339 66 330 1.88e-19 88.6
MS.gene66833.t1 MTR_7g111050 26.667 330 204 15 39 339 7 327 3.31e-17 82.0
MS.gene66833.t1 MTR_8g087510 34.615 130 79 4 173 300 23 148 3.68e-14 70.1
MS.gene66833.t1 MTR_7g079260 44.444 72 23 2 274 344 7 62 1.56e-11 60.8
MS.gene66833.t1 MTR_7g111050 25.510 294 187 12 43 309 8 296 9.38e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66833.t1 AT1G20850 77.439 328 72 2 25 350 29 356 0.0 530
MS.gene66833.t1 AT4G35350 69.014 355 105 2 1 350 1 355 0.0 529
MS.gene66833.t1 AT4G35350 66.901 284 89 2 1 279 1 284 1.79e-143 407
MS.gene66833.t1 AT5G43060 53.333 360 152 5 1 349 1 355 2.21e-129 378
MS.gene66833.t1 AT1G47128 52.101 357 158 5 1 348 1 353 3.38e-129 378
MS.gene66833.t1 AT5G50260 55.072 345 144 5 9 348 5 343 4.05e-129 374
MS.gene66833.t1 AT3G19390 54.070 344 149 6 8 348 9 346 8.40e-126 369
MS.gene66833.t1 AT3G48340 51.471 340 158 3 15 348 6 344 1.24e-118 347
MS.gene66833.t1 AT1G09850 53.074 309 144 1 41 348 26 334 6.31e-115 340
MS.gene66833.t1 AT4G36880 50.155 323 149 4 38 348 40 362 4.62e-112 331
MS.gene66833.t1 AT5G45890 48.974 341 159 7 17 347 10 345 2.39e-111 328
MS.gene66833.t1 AT3G19400 51.948 308 142 6 46 348 43 349 1.39e-108 322
MS.gene66833.t1 AT4G11310 47.175 354 174 6 5 348 3 353 6.88e-108 320
MS.gene66833.t1 AT4G11320 46.348 356 180 6 1 348 8 360 6.81e-107 318
MS.gene66833.t1 AT4G11320 46.348 356 180 6 1 348 8 360 6.81e-107 318
MS.gene66833.t1 AT1G06260 48.538 342 166 5 8 348 11 343 1.48e-106 316
MS.gene66833.t1 AT4G23520 44.729 351 190 4 1 348 1 350 6.72e-106 315
MS.gene66833.t1 AT3G48350 47.688 346 169 6 9 348 5 344 7.72e-106 315
MS.gene66833.t1 AT3G49340 48.232 311 150 6 44 346 32 339 9.60e-100 298
MS.gene66833.t1 AT2G27420 47.634 317 150 6 44 346 32 346 6.34e-98 294
MS.gene66833.t1 AT1G29090 47.771 314 153 6 43 347 43 354 5.24e-96 289
MS.gene66833.t1 AT2G34080 46.474 312 158 5 43 347 35 344 5.06e-95 286
MS.gene66833.t1 AT1G29080 42.566 343 183 9 15 347 7 345 6.04e-92 279
MS.gene66833.t1 AT3G43960 45.370 324 166 9 33 348 27 347 4.55e-86 265
MS.gene66833.t1 AT1G29110 37.647 340 190 6 17 347 7 333 1.20e-80 249
MS.gene66833.t1 AT3G19400 51.899 237 108 6 46 277 43 278 5.94e-77 238
MS.gene66833.t1 AT3G48350 45.161 279 142 5 9 282 5 277 7.86e-75 233
MS.gene66833.t1 AT5G60360 42.671 307 161 9 47 347 59 356 1.59e-74 234
MS.gene66833.t1 AT5G60360 42.345 307 161 10 47 347 59 355 6.43e-72 228
MS.gene66833.t1 AT5G60360 42.667 300 157 9 47 340 59 349 9.03e-72 228
MS.gene66833.t1 AT4G39090 39.940 333 171 9 29 344 37 357 2.75e-70 224
MS.gene66833.t1 AT2G21430 39.634 328 169 9 34 344 39 354 2.82e-69 221
MS.gene66833.t1 AT3G45310 40.717 307 167 9 47 347 59 356 2.81e-67 216
MS.gene66833.t1 AT3G45310 39.474 304 173 7 47 344 59 357 5.05e-65 210
MS.gene66833.t1 AT4G16190 39.241 316 167 8 47 344 55 363 3.52e-61 201
MS.gene66833.t1 AT3G54940 35.655 359 199 9 10 344 9 359 2.39e-60 198
MS.gene66833.t1 AT3G54940 34.201 269 162 4 10 264 9 276 9.51e-46 157
MS.gene66833.t1 AT4G01610 26.562 320 193 15 59 349 36 342 1.21e-16 80.5
MS.gene66833.t1 AT4G01610 26.250 320 194 15 59 349 36 342 1.77e-15 77.0

Find 89 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCAGGTTCGAGGTCTTTA+AGG 0.183217 3.4:+99033661 MS.gene66833:CDS
ACTGATGTTGTGACTATTAA+TGG 0.222069 3.4:+99034657 MS.gene66833:CDS
TCAAATTCCCTGTCACAATT+TGG 0.244699 3.4:-99034359 None:intergenic
GCAGGTCAGGACAAGTGTTT+TGG 0.258810 3.4:-99033483 None:intergenic
GGAAGATTATCCTTACATTA+TGG 0.312016 3.4:+99034500 MS.gene66833:CDS
ATCTCTGCCTGAAGCCTCTA+TGG 0.319266 3.4:-99034751 None:intergenic
ACTTGTTCTTGAACTCTTGA+TGG 0.328420 3.4:-99033763 None:intergenic
ATATGGTACATCAAAGGGTT+TGG 0.369364 3.4:+99034941 MS.gene66833:CDS
TTATTTCTATCTTTAGCATT+TGG 0.374681 3.4:+99033515 MS.gene66833:CDS
CATTTGTAGGGTGTGTTTGA+TGG 0.384261 3.4:+99034876 MS.gene66833:intron
AAGCACTTGCGAGATGAAAA+AGG 0.391386 3.4:+99034527 MS.gene66833:CDS
GAGTACTTGCAGGTAGTTGC+TGG 0.397924 3.4:+99034301 MS.gene66833:intron
AACATACTCCACTATAGAAC+TGG 0.404978 3.4:-99034776 None:intergenic
CATTGACCTTGATTCTTGAC+CGG 0.416938 3.4:-99033902 None:intergenic
ACATTATAGTGAAGAATTCA+TGG 0.418831 3.4:+99034967 MS.gene66833:CDS
TTTCTCCAATCAACTGACTT+AGG 0.421540 3.4:-99033863 None:intergenic
TCAACTGTTGCCGCGGTTGA+AGG 0.428053 3.4:+99034330 MS.gene66833:CDS
ATAAGCATAAGGAGCAGGTC+AGG 0.428907 3.4:-99033496 None:intergenic
GGAGCAAAGTGGGGTGAGAA+AGG 0.429527 3.4:+99034990 MS.gene66833:CDS
ATTTGCCTAAGTCAGTTGAT+TGG 0.430861 3.4:+99033858 MS.gene66833:CDS
CTTACAACAATGGTTGCAAT+GGG 0.434061 3.4:+99034421 MS.gene66833:CDS
GGGGTGAGAAAGGGTTTATA+AGG 0.436382 3.4:+99035000 MS.gene66833:CDS
GGTGTGTCAGCCGTTGGATA+TGG 0.454497 3.4:+99034924 MS.gene66833:CDS
AGTTCAAGAACAAGTATCTT+GGG 0.458742 3.4:+99033771 MS.gene66833:CDS
GAACAAGTATCTTGGGCTAA+AGG 0.462245 3.4:+99033778 MS.gene66833:CDS
GAGTTCAAGAACAAGTATCT+TGG 0.466462 3.4:+99033770 MS.gene66833:CDS
AGAGATTTCCAGTTCTATAG+TGG 0.466790 3.4:+99034768 MS.gene66833:CDS
GGAATAAACCAAATTGTGAC+AGG 0.467218 3.4:+99034351 MS.gene66833:CDS
TCATTGAACTCTTCGAATCA+TGG 0.479297 3.4:+99033594 MS.gene66833:CDS
GAATTCATGGGGAGCAAAGT+GGG 0.481502 3.4:+99034980 MS.gene66833:CDS
TCCATGGACTTCAAGTCCTC+TGG 0.486674 3.4:-99033566 None:intergenic
TAATGAACAAAGCTTATTGA+AGG 0.491336 3.4:+99034701 MS.gene66833:CDS
GAGCAAAGTGGGGTGAGAAA+GGG 0.494121 3.4:+99034991 MS.gene66833:CDS
ATCTAAAGCACATTGATGAT+AGG 0.494574 3.4:+99033687 MS.gene66833:CDS
GTGCTTTCCTCCATAATGTA+AGG 0.494914 3.4:-99034510 None:intergenic
TAGAAATAAGCATAAGGAGC+AGG 0.496949 3.4:-99033501 None:intergenic
ACAAGGTTGTCAGCAACTAC+TGG 0.500291 3.4:+99033711 MS.gene66833:CDS
GGAAAGTCCGAAGGGATTTG+TGG 0.502529 3.4:+99035038 MS.gene66833:CDS
GTTGTCAGCAACTACTGGCT+TGG 0.503373 3.4:+99033716 MS.gene66833:CDS
ATTGACCTTGATTCTTGACC+GGG 0.509041 3.4:-99033901 None:intergenic
TCAGTTGATTGGAGAAAGAA+AGG 0.509647 3.4:+99033869 MS.gene66833:CDS
TAAAGATAGAAATAAGCATA+AGG 0.512917 3.4:-99033507 None:intergenic
GTTGGATATGGTACATCAAA+GGG 0.516544 3.4:+99034936 MS.gene66833:CDS
CTATGGCCACACTGAGTGGT+TGG 0.516563 3.4:-99034734 None:intergenic
CGTTGGATATGGTACATCAA+AGG 0.524173 3.4:+99034935 MS.gene66833:CDS
ATAAGGATGAAGAGAAACAT+TGG 0.526478 3.4:+99035017 MS.gene66833:CDS
CATTATAGTGAAGAATTCAT+GGG 0.532840 3.4:+99034968 MS.gene66833:CDS
ACTTACAACAATGGTTGCAA+TGG 0.533682 3.4:+99034420 MS.gene66833:CDS
GGACGTGATTTCTCCATCGT+GGG 0.535021 3.4:+99033536 MS.gene66833:CDS
GTAGAGTCCACAAATCCCTT+CGG 0.538827 3.4:-99035045 None:intergenic
TACAACAATGGTTGCAATGG+GGG 0.543003 3.4:+99034423 MS.gene66833:CDS
ACCACTCAGTGTGGCCATAG+AGG 0.545434 3.4:+99034737 MS.gene66833:CDS
AAAGAAAGGTGCTGTAACCC+CGG 0.549002 3.4:+99033883 MS.gene66833:CDS
TAAAGGTTGACTTGTCTCAA+AGG 0.550091 3.4:+99033795 MS.gene66833:CDS
GATCATGGTGTGTCAGCCGT+TGG 0.556769 3.4:+99034918 MS.gene66833:CDS
GTAACCCCGGTCAAGAATCA+AGG 0.557737 3.4:+99033896 MS.gene66833:CDS
AAACGGTGGACTACATAAAG+AGG 0.559559 3.4:+99034479 MS.gene66833:CDS
AGTGTGGCCATAGAGGCTTC+AGG 0.567247 3.4:+99034744 MS.gene66833:CDS
GAGTTCAATGAGCTTATCCA+TGG 0.571450 3.4:-99033582 None:intergenic
ACCAGAGGACTTGAAGTCCA+TGG 0.576800 3.4:+99033565 MS.gene66833:CDS
TGGACGTGATTTCTCCATCG+TGG 0.576917 3.4:+99033535 MS.gene66833:CDS
AGAAACATTGGAAAGTCCGA+AGG 0.577636 3.4:+99035029 MS.gene66833:CDS
TGCGGAAGTGAACTAGATCA+TGG 0.579661 3.4:+99034903 MS.gene66833:CDS
CCATTGAAGAAAAGCTACTC+AGG 0.581738 3.4:+99033645 MS.gene66833:CDS
AGAAAAGCTACTCAGGTTCG+AGG 0.582875 3.4:+99033652 MS.gene66833:CDS
AAGGAGAGAGTCCTCAGAAG+AGG 0.591057 3.4:+99033814 MS.gene66833:CDS
AGAATTCATGGGGAGCAAAG+TGG 0.591803 3.4:+99034979 MS.gene66833:CDS
GGCCACACTGAGTGGTTGGT+TGG 0.597846 3.4:-99034730 None:intergenic
ATAAGTGAATTCCTCTTCTG+AGG 0.598495 3.4:-99033825 None:intergenic
AGTACTTGCAGGTAGTTGCT+GGG 0.604719 3.4:+99034302 MS.gene66833:intron
GCCTCTATGGCCACACTGAG+TGG 0.610194 3.4:-99034738 None:intergenic
TTGGTTTATTCCTTCAACCG+CGG 0.610274 3.4:-99034340 None:intergenic
AGATTATCCTTACATTATGG+AGG 0.615877 3.4:+99034503 MS.gene66833:CDS
GATTTGTGGACTCTACAAGA+TGG 0.625513 3.4:+99035052 MS.gene66833:CDS
CTTTGATGTACCATATCCAA+CGG 0.626421 3.4:-99034934 None:intergenic
TGCGACACAACTTACAACAA+TGG 0.635197 3.4:+99034411 MS.gene66833:CDS
GAATCATGGATGTCGAGACA+CGG 0.654814 3.4:+99033608 MS.gene66833:CDS
CTCTGGTGAATAGCCCACGA+TGG 0.663678 3.4:-99033549 None:intergenic
ATTATAGTGAAGAATTCATG+GGG 0.665269 3.4:+99034969 MS.gene66833:CDS
GAAACATTGGAAAGTCCGAA+GGG 0.667328 3.4:+99035030 MS.gene66833:CDS
GCACATTGATGATAGGAACA+AGG 0.668624 3.4:+99033694 MS.gene66833:CDS
AATTCATGGGGAGCAAAGTG+GGG 0.670875 3.4:+99034981 MS.gene66833:CDS
TGCCAACCAACCACTCAGTG+TGG 0.678227 3.4:+99034728 MS.gene66833:CDS
CATCGTGGGCTATTCACCAG+AGG 0.679810 3.4:+99033550 MS.gene66833:CDS
GTCAAGAATCAAGGTCAATG+TGG 0.700857 3.4:+99033905 MS.gene66833:CDS
GAATAAACCAAATTGTGACA+GGG 0.702620 3.4:+99034352 MS.gene66833:CDS
TTACAACAATGGTTGCAATG+GGG 0.703567 3.4:+99034422 MS.gene66833:CDS
TTGACCTTGATTCTTGACCG+GGG 0.717921 3.4:-99033900 None:intergenic
GGTGTGTTTGATGGACATTG+CGG 0.757484 3.4:+99034885 MS.gene66833:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTATTATTTTTTTTAAT+GGG + chr3.4:99034807-99034826 MS.gene66833:intron 0.0%
!!! TTAATTATTATTTTTTTTAA+TGG + chr3.4:99034806-99034825 MS.gene66833:intron 0.0%
!! TTATATATACTATTGGATAA+CGG + chr3.4:99034209-99034228 MS.gene66833:intron 15.0%
!! ATAAGTAACATAACTTTCAT+TGG + chr3.4:99034615-99034634 MS.gene66833:intron 20.0%
!! ATTATTTAACTACCATTTGT+AGG + chr3.4:99034863-99034882 MS.gene66833:intron 20.0%
!! GTTCAGTTTATATATACTAT+TGG + chr3.4:99034202-99034221 MS.gene66833:intron 20.0%
!! TAAAGATAGAAATAAGCATA+AGG - chr3.4:99033510-99033529 None:intergenic 20.0%
!! TTATTTAACTACCATTTGTA+GGG + chr3.4:99034864-99034883 MS.gene66833:intron 20.0%
!!! TTATTTCTATCTTTAGCATT+TGG + chr3.4:99033515-99033534 MS.gene66833:CDS 20.0%
! ACATTATAGTGAAGAATTCA+TGG + chr3.4:99034967-99034986 MS.gene66833:CDS 25.0%
! ATCATTGAATTGATATCAGA+CGG - chr3.4:99034066-99034085 None:intergenic 25.0%
! ATTATAGTGAAGAATTCATG+GGG + chr3.4:99034969-99034988 MS.gene66833:CDS 25.0%
! CATTATAGTGAAGAATTCAT+GGG + chr3.4:99034968-99034987 MS.gene66833:CDS 25.0%
! TAATGAACAAAGCTTATTGA+AGG + chr3.4:99034701-99034720 MS.gene66833:CDS 25.0%
! TTCTCCTTCATTGTAAAAAA+CGG + chr3.4:99034462-99034481 MS.gene66833:CDS 25.0%
!!! AAGCTTTGTTCATTATTTTG+TGG - chr3.4:99034696-99034715 None:intergenic 25.0%
ACTGATGTTGTGACTATTAA+TGG + chr3.4:99034657-99034676 MS.gene66833:CDS 30.0%
AGATTATCCTTACATTATGG+AGG + chr3.4:99034503-99034522 MS.gene66833:CDS 30.0%
AGCGAAAAATTTGCATGATT+AGG - chr3.4:99034146-99034165 None:intergenic 30.0%
AGTTCAAGAACAAGTATCTT+GGG + chr3.4:99033771-99033790 MS.gene66833:CDS 30.0%
ATAAGGATGAAGAGAAACAT+TGG + chr3.4:99035017-99035036 MS.gene66833:CDS 30.0%
GAATAAACCAAATTGTGACA+GGG + chr3.4:99034352-99034371 MS.gene66833:CDS 30.0%
GGAAGATTATCCTTACATTA+TGG + chr3.4:99034500-99034519 MS.gene66833:CDS 30.0%
TAGGCATGTTTACATATAGT+AGG - chr3.4:99034127-99034146 None:intergenic 30.0%
TGGGTGAATCTAATCTTAAA+TGG + chr3.4:99034826-99034845 MS.gene66833:intron 30.0%
! ATCTAAAGCACATTGATGAT+AGG + chr3.4:99033687-99033706 MS.gene66833:CDS 30.0%
AACATACTCCACTATAGAAC+TGG - chr3.4:99034779-99034798 None:intergenic 35.0%
ACTTACAACAATGGTTGCAA+TGG + chr3.4:99034420-99034439 MS.gene66833:CDS 35.0%
ACTTGTTCTTGAACTCTTGA+TGG - chr3.4:99033766-99033785 None:intergenic 35.0%
AGAGATTTCCAGTTCTATAG+TGG + chr3.4:99034768-99034787 MS.gene66833:CDS 35.0%
ATAAGTGAATTCCTCTTCTG+AGG - chr3.4:99033828-99033847 None:intergenic 35.0%
ATATGGTACATCAAAGGGTT+TGG + chr3.4:99034941-99034960 MS.gene66833:CDS 35.0%
ATTTGCCTAAGTCAGTTGAT+TGG + chr3.4:99033858-99033877 MS.gene66833:CDS 35.0%
CTTACAACAATGGTTGCAAT+GGG + chr3.4:99034421-99034440 MS.gene66833:CDS 35.0%
CTTTGATGTACCATATCCAA+CGG - chr3.4:99034937-99034956 None:intergenic 35.0%
GAGTTCAAGAACAAGTATCT+TGG + chr3.4:99033770-99033789 MS.gene66833:CDS 35.0%
GGAATAAACCAAATTGTGAC+AGG + chr3.4:99034351-99034370 MS.gene66833:CDS 35.0%
GTTGGATATGGTACATCAAA+GGG + chr3.4:99034936-99034955 MS.gene66833:CDS 35.0%
TAGAAATAAGCATAAGGAGC+AGG - chr3.4:99033504-99033523 None:intergenic 35.0%
TCAAATTCCCTGTCACAATT+TGG - chr3.4:99034362-99034381 None:intergenic 35.0%
TCAGTTGATTGGAGAAAGAA+AGG + chr3.4:99033869-99033888 MS.gene66833:CDS 35.0%
TCATTGAACTCTTCGAATCA+TGG + chr3.4:99033594-99033613 MS.gene66833:CDS 35.0%
TCCTTCATTGTAAAAAACGG+TGG + chr3.4:99034465-99034484 MS.gene66833:CDS 35.0%
TTACAACAATGGTTGCAATG+GGG + chr3.4:99034422-99034441 MS.gene66833:CDS 35.0%
! TTTCTCCAATCAACTGACTT+AGG - chr3.4:99033866-99033885 None:intergenic 35.0%
!! TAAAGGTTGACTTGTCTCAA+AGG + chr3.4:99033795-99033814 MS.gene66833:CDS 35.0%
!! TTTCATTGGTTTGTTTCACC+AGG + chr3.4:99034629-99034648 MS.gene66833:intron 35.0%
!!! TCCACCGTTTTTTACAATGA+AGG - chr3.4:99034469-99034488 None:intergenic 35.0%
AAGCACTTGCGAGATGAAAA+AGG + chr3.4:99034527-99034546 MS.gene66833:CDS 40.0%
AGAAACATTGGAAAGTCCGA+AGG + chr3.4:99035029-99035048 MS.gene66833:CDS 40.0%
AGCTTGCAAGCATATTGACA+TGG + chr3.4:99034266-99034285 MS.gene66833:intron 40.0%
ATTGACCTTGATTCTTGACC+GGG - chr3.4:99033904-99033923 None:intergenic 40.0%
CATCAAACACACCCTACAAA+TGG - chr3.4:99034878-99034897 None:intergenic 40.0%
CATTGACCTTGATTCTTGAC+CGG - chr3.4:99033905-99033924 None:intergenic 40.0%
CATTTGTAGGGTGTGTTTGA+TGG + chr3.4:99034876-99034895 MS.gene66833:intron 40.0%
CCATTGAAGAAAAGCTACTC+AGG + chr3.4:99033645-99033664 MS.gene66833:CDS 40.0%
CGTTGGATATGGTACATCAA+AGG + chr3.4:99034935-99034954 MS.gene66833:CDS 40.0%
GAAACATTGGAAAGTCCGAA+GGG + chr3.4:99035030-99035049 MS.gene66833:CDS 40.0%
GAACAAGTATCTTGGGCTAA+AGG + chr3.4:99033778-99033797 MS.gene66833:CDS 40.0%
GAGTTCAATGAGCTTATCCA+TGG - chr3.4:99033585-99033604 None:intergenic 40.0%
GATTTGTGGACTCTACAAGA+TGG + chr3.4:99035052-99035071 MS.gene66833:CDS 40.0%
GTCAAGAATCAAGGTCAATG+TGG + chr3.4:99033905-99033924 MS.gene66833:CDS 40.0%
GTGCTTTCCTCCATAATGTA+AGG - chr3.4:99034513-99034532 None:intergenic 40.0%
TACAACAATGGTTGCAATGG+GGG + chr3.4:99034423-99034442 MS.gene66833:CDS 40.0%
TCACAACATCAGTTACTTCC+TGG - chr3.4:99034650-99034669 None:intergenic 40.0%
TGCGACACAACTTACAACAA+TGG + chr3.4:99034411-99034430 MS.gene66833:CDS 40.0%
TTGCAAGCATATTGACATGG+TGG + chr3.4:99034269-99034288 MS.gene66833:intron 40.0%
TTGGTTTATTCCTTCAACCG+CGG - chr3.4:99034343-99034362 None:intergenic 40.0%
! CCTGAGTAGCTTTTCTTCAA+TGG - chr3.4:99033648-99033667 None:intergenic 40.0%
! GCACATTGATGATAGGAACA+AGG + chr3.4:99033694-99033713 MS.gene66833:CDS 40.0%
!! AAACGGTGGACTACATAAAG+AGG + chr3.4:99034479-99034498 MS.gene66833:CDS 40.0%
AATTCATGGGGAGCAAAGTG+GGG + chr3.4:99034981-99035000 MS.gene66833:CDS 45.0%
ACAAGGTTGTCAGCAACTAC+TGG + chr3.4:99033711-99033730 MS.gene66833:CDS 45.0%
ACTCAGGTTCGAGGTCTTTA+AGG + chr3.4:99033661-99033680 MS.gene66833:CDS 45.0%
AGAAAAGCTACTCAGGTTCG+AGG + chr3.4:99033652-99033671 MS.gene66833:CDS 45.0%
AGAATTCATGGGGAGCAAAG+TGG + chr3.4:99034979-99034998 MS.gene66833:CDS 45.0%
AGTACTTGCAGGTAGTTGCT+GGG + chr3.4:99034302-99034321 MS.gene66833:intron 45.0%
ATAAGCATAAGGAGCAGGTC+AGG - chr3.4:99033499-99033518 None:intergenic 45.0%
GAATCATGGATGTCGAGACA+CGG + chr3.4:99033608-99033627 MS.gene66833:CDS 45.0%
GAATTCATGGGGAGCAAAGT+GGG + chr3.4:99034980-99034999 MS.gene66833:CDS 45.0%
GGGGTGAGAAAGGGTTTATA+AGG + chr3.4:99035000-99035019 MS.gene66833:CDS 45.0%
GTAGAGTCCACAAATCCCTT+CGG - chr3.4:99035048-99035067 None:intergenic 45.0%
GTATGAACACGAGTACTTGC+AGG + chr3.4:99034291-99034310 MS.gene66833:intron 45.0%
TGCGGAAGTGAACTAGATCA+TGG + chr3.4:99034903-99034922 MS.gene66833:CDS 45.0%
TTGACCTTGATTCTTGACCG+GGG - chr3.4:99033903-99033922 None:intergenic 45.0%
! AGGTCAGGACAAGTGTTTTG+GGG - chr3.4:99033484-99033503 None:intergenic 45.0%
! CAGGTCAGGACAAGTGTTTT+GGG - chr3.4:99033485-99033504 None:intergenic 45.0%
! GGTGTGTTTGATGGACATTG+CGG + chr3.4:99034885-99034904 MS.gene66833:CDS 45.0%
!! AAAGAAAGGTGCTGTAACCC+CGG + chr3.4:99033883-99033902 MS.gene66833:CDS 45.0%
AAGGAGAGAGTCCTCAGAAG+AGG + chr3.4:99033814-99033833 MS.gene66833:CDS 50.0%
ACCAGAGGACTTGAAGTCCA+TGG + chr3.4:99033565-99033584 MS.gene66833:CDS 50.0%
ATCTCTGCCTGAAGCCTCTA+TGG - chr3.4:99034754-99034773 None:intergenic 50.0%
CATCCACATAGTCGATGCAC+TGG + chr3.4:99033983-99034002 MS.gene66833:intron 50.0%
GAGCAAAGTGGGGTGAGAAA+GGG + chr3.4:99034991-99035010 MS.gene66833:CDS 50.0%
GAGTACTTGCAGGTAGTTGC+TGG + chr3.4:99034301-99034320 MS.gene66833:intron 50.0%
GGAAAGTCCGAAGGGATTTG+TGG + chr3.4:99035038-99035057 MS.gene66833:CDS 50.0%
GGACGTGATTTCTCCATCGT+GGG + chr3.4:99033536-99033555 MS.gene66833:CDS 50.0%
GTAACCCCGGTCAAGAATCA+AGG + chr3.4:99033896-99033915 MS.gene66833:CDS 50.0%
TCCATGGACTTCAAGTCCTC+TGG - chr3.4:99033569-99033588 None:intergenic 50.0%
TGGACGTGATTTCTCCATCG+TGG + chr3.4:99033535-99033554 MS.gene66833:CDS 50.0%
TGGTTGCAATGGGGGTCTAA+TGG + chr3.4:99034431-99034450 MS.gene66833:CDS 50.0%
! GCAGGTCAGGACAAGTGTTT+TGG - chr3.4:99033486-99033505 None:intergenic 50.0%
! GGCATTTTCAACTGTTGCCG+CGG + chr3.4:99034323-99034342 MS.gene66833:CDS 50.0%
! GTTGTCAGCAACTACTGGCT+TGG + chr3.4:99033716-99033735 MS.gene66833:CDS 50.0%
!! GAACCAGTGCATCGACTATG+TGG - chr3.4:99033989-99034008 None:intergenic 50.0%
!! GGTCAGGACAAGTGTTTTGG+GGG - chr3.4:99033483-99033502 None:intergenic 50.0%
!! GTGCATCGACTATGTGGATG+CGG - chr3.4:99033983-99034002 None:intergenic 50.0%
!! TGCATCGACTATGTGGATGC+GGG - chr3.4:99033982-99034001 None:intergenic 50.0%
ACCACTCAGTGTGGCCATAG+AGG + chr3.4:99034737-99034756 MS.gene66833:CDS 55.0%
AGTGTGGCCATAGAGGCTTC+AGG + chr3.4:99034744-99034763 MS.gene66833:CDS 55.0%
CTATGGCCACACTGAGTGGT+TGG - chr3.4:99034737-99034756 None:intergenic 55.0%
CTCTGGTGAATAGCCCACGA+TGG - chr3.4:99033552-99033571 None:intergenic 55.0%
GATCATGGTGTGTCAGCCGT+TGG + chr3.4:99034918-99034937 MS.gene66833:CDS 55.0%
GGAGCAAAGTGGGGTGAGAA+AGG + chr3.4:99034990-99035009 MS.gene66833:CDS 55.0%
GGTGTGTCAGCCGTTGGATA+TGG + chr3.4:99034924-99034943 MS.gene66833:CDS 55.0%
TCAACTGTTGCCGCGGTTGA+AGG + chr3.4:99034330-99034349 MS.gene66833:CDS 55.0%
TGCCAACCAACCACTCAGTG+TGG + chr3.4:99034728-99034747 MS.gene66833:CDS 55.0%
! CATCGTGGGCTATTCACCAG+AGG + chr3.4:99033550-99033569 MS.gene66833:CDS 55.0%
!! GCATCGACTATGTGGATGCG+GGG - chr3.4:99033981-99034000 None:intergenic 55.0%
GCCTCTATGGCCACACTGAG+TGG - chr3.4:99034741-99034760 None:intergenic 60.0%
GGCCACACTGAGTGGTTGGT+TGG - chr3.4:99034733-99034752 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 99033467 99035100 99033467 ID=MS.gene66833
chr3.4 mRNA 99033467 99035100 99033467 ID=MS.gene66833.t1;Parent=MS.gene66833
chr3.4 exon 99033467 99033926 99033467 ID=MS.gene66833.t1.exon1;Parent=MS.gene66833.t1
chr3.4 CDS 99033467 99033926 99033467 ID=cds.MS.gene66833.t1;Parent=MS.gene66833.t1
chr3.4 exon 99034313 99034548 99034313 ID=MS.gene66833.t1.exon2;Parent=MS.gene66833.t1
chr3.4 CDS 99034313 99034548 99034313 ID=cds.MS.gene66833.t1;Parent=MS.gene66833.t1
chr3.4 exon 99034651 99034791 99034651 ID=MS.gene66833.t1.exon3;Parent=MS.gene66833.t1
chr3.4 CDS 99034651 99034791 99034651 ID=cds.MS.gene66833.t1;Parent=MS.gene66833.t1
chr3.4 exon 99034885 99035100 99034885 ID=MS.gene66833.t1.exon4;Parent=MS.gene66833.t1
chr3.4 CDS 99034885 99035100 99034885 ID=cds.MS.gene66833.t1;Parent=MS.gene66833.t1
Gene Sequence

>MS.gene66833

ATGGCTTTTTTCTCCCCCAAAACACTTGTCCTGACCTGCTCCTTATGCTTATTTCTATCTTTAGCATTTGGACGTGATTTCTCCATCGTGGGCTATTCACCAGAGGACTTGAAGTCCATGGATAAGCTCATTGAACTCTTCGAATCATGGATGTCGAGACACGGAAAGATTTATGAGACCATTGAAGAAAAGCTACTCAGGTTCGAGGTCTTTAAGGATAATCTAAAGCACATTGATGATAGGAACAAGGTTGTCAGCAACTACTGGCTTGGTTTGAATGAGTTTGCTGATTTGAGCCATCAAGAGTTCAAGAACAAGTATCTTGGGCTAAAGGTTGACTTGTCTCAAAGGAGAGAGTCCTCAGAAGAGGAATTCACTTATAGAGATGTTGATTTGCCTAAGTCAGTTGATTGGAGAAAGAAAGGTGCTGTAACCCCGGTCAAGAATCAAGGTCAATGTGGTAAGTATACTATTATTGCTATATATGAAAATCTATCTATTCTTGAATATTCCCCGCATCCACATAGTCGATGCACTGGTTCTCTTTTGATTCTTTTTGATAAGTTAAAATTAAAATACTTGATTTAAAATTTGAACCGTCTGATATCAATTCAATGATTATTGATTTGTTCATTGCATGAATTCAGCGTATTTCATCCTACTATATGTAAACATGCCTAATCATGCAAATTTTTCGCTATATGTTAGATTAATTTTTCAGTTAAATTTGAAATAGTTCAGTTTATATATACTATTGGATAACGGCACACAAGAAAATGCTTAAGTTTTGTTTAGAAATAGCTTGCAAGCATATTGACATGGTGGTATGAACACGAGTACTTGCAGGTAGTTGCTGGGCATTTTCAACTGTTGCCGCGGTTGAAGGAATAAACCAAATTGTGACAGGGAATTTGACATCGTTGTCTGAGCAAGAATTAATCGACTGCGACACAACTTACAACAATGGTTGCAATGGGGGTCTAATGGACTATGCATTCTCCTTCATTGTAAAAAACGGTGGACTACATAAAGAGGAAGATTATCCTTACATTATGGAGGAAAGCACTTGCGAGATGAAAAAGGTACATATAGATTAATTAAGCTAACAAATTCAATCTAAAAACATATTGTTTCATACTAAAGTTATTATAAGTAACATAACTTTCATTGGTTTGTTTCACCAGGAAGTAACTGATGTTGTGACTATTAATGGATATCATGACGTGCCACAAAATAATGAACAAAGCTTATTGAAGGCACTTGCCAACCAACCACTCAGTGTGGCCATAGAGGCTTCAGGCAGAGATTTCCAGTTCTATAGTGGAGTATGTTTAATTAATTAATTATTATTTTTTTTAATGGGTGAATCTAATCTTAAATGGAATTTTTTGAACTGATTATTTAACTACCATTTGTAGGGTGTGTTTGATGGACATTGCGGAAGTGAACTAGATCATGGTGTGTCAGCCGTTGGATATGGTACATCAAAGGGTTTGGATTACATTATAGTGAAGAATTCATGGGGAGCAAAGTGGGGTGAGAAAGGGTTTATAAGGATGAAGAGAAACATTGGAAAGTCCGAAGGGATTTGTGGACTCTACAAGATGGCTTCTTATCCCACTAAAAAGAAATAA
Protein sequence

>MS.gene66833.t1

MAFFSPKTLVLTCSLCLFLSLAFGRDFSIVGYSPEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDDRNKVVSNYWLGLNEFADLSHQEFKNKYLGLKVDLSQRRESSEEEFTYRDVDLPKSVDWRKKGAVTPVKNQGQCGSCWAFSTVAAVEGINQIVTGNLTSLSEQELIDCDTTYNNGCNGGLMDYAFSFIVKNGGLHKEEDYPYIMEESTCEMKKEVTDVVTINGYHDVPQNNEQSLLKALANQPLSVAIEASGRDFQFYSGGVFDGHCGSELDHGVSAVGYGTSKGLDYIIVKNSWGAKWGEKGFIRMKRNIGKSEGICGLYKMASYPTKKK