AlfalfaGEDB | MS.gene29082

M. sativa cultivar XinJiangDaYe / MS.gene29082

KEGG: K16292
GO: GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00112.26 PF08246.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29082.t1	XP_013460973.1	96.4	362	13	0	1	362	1	362	1.10E-202	715.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29082.t1	P12412	82.0	362	65	0	1	362	1	362	7.5e-176	617.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29082.t1	G8A2F7	96.4	362	13	0	1	362	1	362	7.9e-203	715.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049227	MS.gene29082	0.827802	1.28E-54	-1.69E-46
MS.gene049807	MS.gene29082	0.815354	9.65E-52	-1.69E-46
MS.gene051279	MS.gene29082	0.807438	5.05E-50	-1.69E-46
MS.gene052155	MS.gene29082	-0.800331	1.52E-48	-1.69E-46
MS.gene052239	MS.gene29082	-0.805391	1.37E-49	-1.69E-46
MS.gene052628	MS.gene29082	0.987764	1.89E-171	-1.69E-46
MS.gene052630	MS.gene29082	0.947645	4.49E-106	-1.69E-46
MS.gene053143	MS.gene29082	0.813825	2.10E-51	-1.69E-46
MS.gene05894	MS.gene29082	0.810877	9.27E-51	-1.69E-46
MS.gene061100	MS.gene29082	0.822668	2.09E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene29082	MS.gene031619	PPI
MS.gene29082	MS.gene045526	PPI
MS.gene29082	MS.gene031621	PPI
MS.gene29082	MS.gene045520	PPI
MS.gene29082	MS.gene67468	PPI
MS.gene29082	MS.gene045459	PPI
MS.gene29082	MS.gene73838	PPI
MS.gene29082	MS.gene043473	PPI
MS.gene026874	MS.gene29082	PPI
MS.gene66833	MS.gene29082	PPI
MS.gene29082	MS.gene045525	PPI
MS.gene29082	MS.gene031635	PPI
MS.gene29082	MS.gene006680	PPI
MS.gene006646	MS.gene29082	PPI
MS.gene065785	MS.gene29082	PPI
MS.gene29082	MS.gene63719	PPI
MS.gene29082	MS.gene058156	PPI
MS.gene060218	MS.gene29082	PPI
MS.gene29082	MS.gene31410	PPI
MS.gene29082	MS.gene045458	PPI
MS.gene29082	MS.gene73815	PPI
MS.gene29082	MS.gene95543	PPI
MS.gene29082	MS.gene34090	PPI
MS.gene29082	MS.gene006720	PPI
MS.gene014304	MS.gene29082	PPI
MS.gene29082	MS.gene045501	PPI
MS.gene29082	MS.gene012206	PPI
MS.gene29082	MS.gene055760	PPI
MS.gene29082	MS.gene50738	PPI
MS.gene29082	MS.gene006665	PPI
MS.gene29082	MS.gene069978	PPI
MS.gene29082	MS.gene064873	PPI
MS.gene29082	MS.gene003680	PPI
MS.gene028850	MS.gene29082	PPI
MS.gene29082	MS.gene003677	PPI
MS.gene29082	MS.gene26670	PPI
MS.gene29082	MS.gene026873	PPI
MS.gene29082	MS.gene005934	PPI
MS.gene29082	MS.gene26669	PPI
MS.gene29082	MS.gene065787	PPI
MS.gene29082	MS.gene59493	PPI
MS.gene29082	MS.gene73817	PPI
MS.gene031580	MS.gene29082	PPI
MS.gene51064	MS.gene29082	PPI
MS.gene29082	MS.gene63396	PPI
MS.gene026876	MS.gene29082	PPI
MS.gene29082	MS.gene64795	PPI
MS.gene23321	MS.gene29082	PPI
MS.gene69195	MS.gene29082	PPI
MS.gene29082	MS.gene031638	PPI
MS.gene027420	MS.gene29082	PPI
MS.gene29082	MS.gene73835	PPI
MS.gene29082	MS.gene26853	PPI
MS.gene29082	MS.gene005933	PPI
MS.gene73834	MS.gene29082	PPI
MS.gene29082	MS.gene045503	PPI
MS.gene29082	MS.gene031581	PPI
MS.gene006682	MS.gene29082	PPI
MS.gene29082	MS.gene73840	PPI
MS.gene29082	MS.gene045461	PPI
MS.gene29082	MS.gene09529	PPI
MS.gene29082	MS.gene033151	PPI
MS.gene29082	MS.gene045522	PPI
MS.gene29082	MS.gene25255	PPI
MS.gene29082	MS.gene045455	PPI
MS.gene29082	MS.gene24818	PPI
MS.gene29082	MS.gene87639	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29082.t1	MTR_3g075390	96.409	362	13	0	1	362	1	362	0.0	737
MS.gene29082.t1	MTR_4g104370	76.243	362	84	2	1	362	1	360	0.0	592
MS.gene29082.t1	MTR_4g080700	57.692	312	121	7	35	343	35	338	5.70e-125	363
MS.gene29082.t1	MTR_4g080730	57.051	312	123	7	35	343	35	338	1.85e-124	362
MS.gene29082.t1	MTR_4g079770	52.586	348	155	7	1	343	1	343	2.47e-124	362
MS.gene29082.t1	MTR_5g022560	55.660	318	127	6	33	343	32	342	6.24e-124	360
MS.gene29082.t1	MTR_4g047610	57.962	314	121	7	38	344	33	342	1.44e-123	360
MS.gene29082.t1	MTR_4g079470	55.312	320	134	6	28	343	29	343	1.92e-123	359
MS.gene29082.t1	MTR_4g107930	50.568	352	156	7	1	343	1	343	3.69e-123	358
MS.gene29082.t1	MTR_1g107550	55.215	326	141	5	23	345	33	356	4.10e-122	360
MS.gene29082.t1	MTR_4g079800	53.395	324	134	6	28	343	29	343	1.25e-121	355
MS.gene29082.t1	MTR_3g116080	51.884	345	155	5	7	345	9	348	1.49e-121	355
MS.gene29082.t1	MTR_1g018840	51.576	349	160	6	6	346	16	363	1.62e-121	358
MS.gene29082.t1	MTR_4g094918	51.320	341	151	5	8	345	14	342	2.18e-121	355
MS.gene29082.t1	MTR_1g018840	51.576	349	160	6	6	346	16	363	4.32e-121	358
MS.gene29082.t1	MTR_3g102220	51.989	352	156	8	6	345	12	362	3.48e-120	355
MS.gene29082.t1	MTR_2g075830	54.227	343	142	7	6	343	7	339	1.03e-118	347
MS.gene29082.t1	MTR_4g079440	50.000	344	156	6	6	344	12	344	8.31e-117	342
MS.gene29082.t1	MTR_2g090890	49.138	348	165	5	1	346	1	338	6.81e-115	337
MS.gene29082.t1	MTR_4g080360	51.792	307	144	3	40	344	37	341	7.07e-113	332
MS.gene29082.t1	MTR_2g083930	46.313	339	171	5	10	344	12	343	2.39e-105	313
MS.gene29082.t1	MTR_7g078600	46.483	327	158	7	25	347	27	340	1.48e-99	298
MS.gene29082.t1	MTR_3g112430	48.875	311	156	3	39	347	35	344	5.89e-98	298
MS.gene29082.t1	MTR_7g079230	45.588	340	170	7	7	343	7	334	7.02e-98	294
MS.gene29082.t1	MTR_2g016180	43.636	330	168	11	25	345	36	356	8.88e-83	260
MS.gene29082.t1	MTR_2g016270	43.161	329	156	10	25	345	36	341	2.02e-82	259
MS.gene29082.t1	MTR_2g015850	44.966	298	158	5	48	344	71	363	2.80e-81	252
MS.gene29082.t1	MTR_4g065077	43.380	355	175	13	6	345	14	357	4.13e-76	239
MS.gene29082.t1	MTR_4g125300	44.295	298	146	9	53	344	65	348	2.33e-73	231
MS.gene29082.t1	MTR_2g015920	39.826	344	187	12	27	361	41	373	7.55e-73	231
MS.gene29082.t1	MTR_8g086470	41.714	350	172	12	6	345	30	357	1.43e-68	219
MS.gene29082.t1	MTR_2g015930	37.978	366	186	16	6	345	9	359	1.62e-65	212
MS.gene29082.t1	MTR_2g067900	41.860	301	158	11	55	344	32	326	1.31e-62	203
MS.gene29082.t1	MTR_1g023210	39.465	299	153	9	54	336	66	352	2.29e-61	201
MS.gene29082.t1	MTR_4g071060	37.793	299	157	8	54	336	63	348	1.29e-58	194
MS.gene29082.t1	MTR_2g067560	40.123	324	153	15	40	338	16	323	1.89e-58	203
MS.gene29082.t1	MTR_2g064475	37.609	343	164	13	40	342	16	348	2.17e-57	190
MS.gene29082.t1	MTR_1g023810	42.731	227	122	6	119	342	40	261	2.82e-56	185
MS.gene29082.t1	MTR_7g078630	42.652	279	112	10	40	304	706	950	3.55e-54	192
MS.gene29082.t1	MTR_1g094135	32.258	341	196	10	15	336	34	358	1.57e-52	179
MS.gene29082.t1	MTR_2g066980	34.853	307	143	11	55	343	50	317	2.99e-52	176
MS.gene29082.t1	MTR_4g072790	31.805	349	188	9	1	336	1	312	7.17e-44	154
MS.gene29082.t1	MTR_2g015840	31.429	315	144	8	32	343	25	270	1.06e-43	152
MS.gene29082.t1	MTR_7g078580	64.646	99	34	1	246	344	4	101	5.23e-43	145
MS.gene29082.t1	MTR_4g072340	34.021	291	147	9	54	336	63	316	7.32e-41	146
MS.gene29082.t1	MTR_4g073660	34.364	291	146	10	54	336	62	315	7.78e-41	146
MS.gene29082.t1	MTR_7g079130	64.835	91	32	0	254	344	7	97	5.84e-38	132
MS.gene29082.t1	MTR_2g047960	77.215	79	18	0	137	215	7	85	1.07e-35	125
MS.gene29082.t1	MTR_1g025450	41.954	174	77	7	127	296	54	207	1.97e-32	127
MS.gene29082.t1	MTR_2g016380	45.902	122	59	4	228	345	18	136	3.28e-28	110
MS.gene29082.t1	MTR_7g111060	27.536	276	164	11	86	336	66	330	4.94e-24	101
MS.gene29082.t1	MTR_7g111050	30.738	244	137	10	130	350	106	340	5.58e-23	99.0
MS.gene29082.t1	MTR_3g029560	32.547	212	114	8	139	344	49	237	7.21e-21	90.5
MS.gene29082.t1	MTR_7g079260	56.944	72	15	1	270	341	7	62	2.22e-20	85.5
MS.gene29082.t1	MTR_7g078310	34.857	175	95	6	48	217	84	244	7.28e-19	85.5
MS.gene29082.t1	MTR_8g087510	41.538	130	70	5	169	296	23	148	7.60e-19	83.6
MS.gene29082.t1	MTR_7g111050	29.648	199	117	7	130	307	101	297	2.96e-15	75.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29082.t1	AT5G50260	70.833	360	104	1	4	362	2	361	0.0	556
MS.gene29082.t1	AT3G48340	66.759	361	117	2	4	362	2	361	0.0	514
MS.gene29082.t1	AT3G48350	63.611	360	129	2	4	362	2	360	2.98e-176	494
MS.gene29082.t1	AT3G48350	63.406	276	100	1	4	278	2	277	6.47e-133	382
MS.gene29082.t1	AT4G35350	51.862	349	158	4	3	345	9	353	1.86e-122	357
MS.gene29082.t1	AT1G20850	54.658	322	142	3	25	345	36	354	3.81e-122	357
MS.gene29082.t1	AT1G47128	48.991	347	166	4	6	346	13	354	1.99e-117	348
MS.gene29082.t1	AT5G43060	50.286	350	158	5	6	345	11	354	2.58e-117	348
MS.gene29082.t1	AT3G19390	48.159	353	174	6	1	350	5	351	1.17e-115	343
MS.gene29082.t1	AT5G45890	47.714	350	165	7	6	343	1	344	2.72e-115	338
MS.gene29082.t1	AT4G36880	51.368	329	149	6	32	351	41	367	7.85e-114	336
MS.gene29082.t1	AT1G06260	48.571	350	164	7	1	345	5	343	1.43e-107	319
MS.gene29082.t1	AT3G19400	51.592	314	142	7	39	347	43	351	1.44e-107	320
MS.gene29082.t1	AT4G11310	46.797	359	170	8	3	347	4	355	1.36e-105	315
MS.gene29082.t1	AT4G11320	46.499	357	169	8	6	347	13	362	2.96e-103	309
MS.gene29082.t1	AT4G11320	46.499	357	169	8	6	347	13	362	2.96e-103	309
MS.gene29082.t1	AT2G27420	45.533	347	174	5	6	344	8	347	3.98e-99	298
MS.gene29082.t1	AT4G23520	46.835	316	158	6	39	350	46	355	1.01e-98	297
MS.gene29082.t1	AT1G09850	48.710	310	152	4	38	345	30	334	1.26e-98	299
MS.gene29082.t1	AT3G49340	44.118	340	183	5	9	344	4	340	1.73e-97	293
MS.gene29082.t1	AT2G34080	44.348	345	181	6	6	344	5	344	4.38e-94	285
MS.gene29082.t1	AT1G29090	44.444	360	169	11	1	344	10	354	2.35e-90	275
MS.gene29082.t1	AT4G35350	49.645	282	132	4	3	278	9	286	5.14e-88	267
MS.gene29082.t1	AT3G43960	45.538	325	161	11	39	354	40	357	5.59e-88	270
MS.gene29082.t1	AT1G29080	41.311	351	193	8	1	344	1	345	1.42e-87	268
MS.gene29082.t1	AT1G29110	41.693	319	165	7	32	344	30	333	1.69e-78	244
MS.gene29082.t1	AT3G19400	50.000	240	111	6	39	273	43	278	6.12e-72	226
MS.gene29082.t1	AT5G60360	42.333	300	153	9	51	344	71	356	5.16e-70	223
MS.gene29082.t1	AT3G45310	41.391	302	153	10	51	344	71	356	1.32e-67	217
MS.gene29082.t1	AT5G60360	42.000	300	153	10	51	344	71	355	1.77e-67	217
MS.gene29082.t1	AT3G45310	41.060	302	153	11	51	344	71	355	4.49e-65	211
MS.gene29082.t1	AT5G60360	42.143	280	146	8	51	324	71	340	7.70e-65	210
MS.gene29082.t1	AT2G21430	34.091	352	198	10	5	336	12	349	4.52e-61	200
MS.gene29082.t1	AT4G39090	37.458	299	159	8	54	336	66	352	1.22e-57	191
MS.gene29082.t1	AT3G54940	38.127	299	159	9	54	336	66	354	2.62e-55	185
MS.gene29082.t1	AT4G16190	37.919	298	159	9	55	336	71	358	9.28e-54	181
MS.gene29082.t1	AT3G54940	38.140	215	121	4	54	260	66	276	4.10e-39	140
MS.gene29082.t1	AT1G02305	27.596	337	209	11	50	362	33	358	1.09e-26	109
MS.gene29082.t1	AT4G01610	29.352	293	166	13	85	350	65	343	3.27e-23	99.8
MS.gene29082.t1	AT4G01610	28.669	293	168	13	85	350	65	343	3.05e-21	94.0
MS.gene29082.t1	AT2G22160	46.465	99	49	1	53	151	8	102	9.60e-21	86.7
MS.gene29082.t1	AT1G02300	31.381	239	132	10	147	362	146	375	3.16e-20	91.3

Find 87 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAACCTATCTGTCTTTATT+TGG	0.189761	3.4:+73820600	None:intergenic
CCGTTGATGGAACTAATTAT+TGG	0.225728	3.4:-73819950	MS.gene29082:CDS
TTTGAGTACATTAAGCAAAA+AGG	0.295259	3.4:-73820497	MS.gene29082:CDS
ATCAGATCCTCCGGCATCTA+TGG	0.300046	3.4:+73820216	None:intergenic
ACATGACCAACCATGAATTT+AGG	0.309976	3.4:-73821182	MS.gene29082:CDS
TGTCCACAACACCAACAAAA+TGG	0.326241	3.4:-73821241	MS.gene29082:CDS
GAACTCATGGGGAGCGGAAT+GGG	0.328427	3.4:-73819919	MS.gene29082:CDS
CTGAGGACACATCTTTATCT+TGG	0.332566	3.4:+73821373	None:intergenic
AAGCTCCTGCTTCTGTTGAT+TGG	0.348923	3.4:-73821068	MS.gene29082:CDS
CTATTACAGGGAGTATTTAC+TGG	0.367645	3.4:-73820026	MS.gene29082:intron
GCATCTATGGCTACGGATAC+AGG	0.385812	3.4:+73820229	None:intergenic
TCAACTGTTGTAGCTGTTGA+AGG	0.388321	3.4:-73820629	MS.gene29082:CDS
TTGTTAAGTTTCAACTTGTA+AGG	0.389898	3.4:+73821213	None:intergenic
AGGATGCAATGGTGGGCTAA+TGG	0.392108	3.4:-73820528	MS.gene29082:CDS
GATCCATCATTTGCTGTGTA+AGG	0.397020	3.4:+73820449	None:intergenic
GTCCCAGCTGTATCAATTGA+TGG	0.409945	3.4:-73820310	MS.gene29082:CDS
CACTTCCACGTGGCGTGCCT+CGG	0.411657	3.4:+73821118	None:intergenic
GATTGTTCTGTCCATTGCTT+TGG	0.412192	3.4:-73821424	MS.gene29082:CDS
ATCAACAGAAGCAGGAGCTT+TGG	0.419799	3.4:+73821071	None:intergenic
TGACCATCAATTGATACAGC+TGG	0.435646	3.4:+73820307	None:intergenic
GGTGTAGCAATAGTAGGATA+TGG	0.443941	3.4:-73819978	MS.gene29082:CDS
TGATGGTCATGAGACTGTTC+CGG	0.449890	3.4:-73820293	MS.gene29082:CDS
GGAGTCACCACACGGTTTCT+CGG	0.450148	3.4:-73821317	MS.gene29082:CDS
GGATATGGAACAACCGTTGA+TGG	0.454887	3.4:-73819963	MS.gene29082:CDS
CGAGGCACGCCACGTGGAAG+TGG	0.460548	3.4:-73821117	MS.gene29082:CDS
TCATCCTTGGTAGATGATAA+AGG	0.462977	3.4:+73819783	None:intergenic
TAAACCAAATAAAGACAGAT+AGG	0.464117	3.4:-73820604	MS.gene29082:CDS
TTCCTCCAATCAACAGAAGC+AGG	0.475830	3.4:+73821063	None:intergenic
CATAGGTAGTCCTAAATTCA+TGG	0.476124	3.4:+73821172	None:intergenic
CTCCTGCTTCTGTTGATTGG+AGG	0.481655	3.4:-73821065	MS.gene29082:CDS
ATTGGATTGTGAGGAACTCA+TGG	0.493389	3.4:-73819932	MS.gene29082:CDS
AATCATGGTGTAGCAATAGT+AGG	0.495226	3.4:-73819984	MS.gene29082:CDS
GTCACTGAGGTCAAGGATCA+AGG	0.495703	3.4:-73821030	MS.gene29082:CDS
TCTAACAAGGAAGGACTTTG+TGG	0.498260	3.4:-73819861	MS.gene29082:CDS
GTAGATGATAAAGGTCCTGC+TGG	0.507201	3.4:+73819792	None:intergenic
AAACATTCAAAGCTCATCCT+TGG	0.508057	3.4:+73819770	None:intergenic
TACCCTTACACAGCAAATGA+TGG	0.512248	3.4:-73820452	MS.gene29082:CDS
GAATTTAGGACTACCTATGC+TGG	0.513702	3.4:-73821168	MS.gene29082:CDS
GTCCTCAGACAAAAGCTTGT+GGG	0.513806	3.4:-73821358	MS.gene29082:CDS
GGAACTCATGGGGAGCGGAA+TGG	0.524402	3.4:-73819920	MS.gene29082:CDS
AGGACTTTGTGGCATAGCTA+TGG	0.526821	3.4:-73819850	MS.gene29082:CDS
TGTGACAATCAAGAAAATCA+AGG	0.527505	3.4:-73820548	MS.gene29082:CDS
GAAAATCAAGGATGCAATGG+TGG	0.535112	3.4:-73820536	MS.gene29082:CDS
TCCTCCGGCATCTATGGCTA+CGG	0.543132	3.4:+73820222	None:intergenic
GACTACCTATGCTGGATCAA+AGG	0.546319	3.4:-73821160	MS.gene29082:CDS
CAAGAAAATCAAGGATGCAA+TGG	0.548537	3.4:-73820539	MS.gene29082:CDS
AAACTGGAAATCAGATCCTC+CGG	0.552551	3.4:+73820207	None:intergenic
TCTGTTGATTGGAGGAAGAA+AGG	0.553167	3.4:-73821057	MS.gene29082:CDS
GGCTACGGATACAGGTTGGT+TGG	0.553342	3.4:+73820237	None:intergenic
AGGTGCTGTCACTGAGGTCA+AGG	0.558185	3.4:-73821037	MS.gene29082:CDS
ATCCATCATTTGCTGTGTAA+GGG	0.560080	3.4:+73820450	None:intergenic
TGTGGTAAAGAGCTAAATCA+TGG	0.564153	3.4:-73819999	MS.gene29082:CDS
CCAATAATTAGTTCCATCAA+CGG	0.573198	3.4:+73819950	None:intergenic
TGTGGGACTTATACGAGAGA+TGG	0.573304	3.4:-73821341	MS.gene29082:CDS
TTGGATTGTGAGGAACTCAT+GGG	0.574375	3.4:-73819931	MS.gene29082:CDS
AACTCATGGGGAGCGGAATG+GGG	0.577276	3.4:-73819918	MS.gene29082:CDS
GACCATCAATTGATACAGCT+GGG	0.584179	3.4:+73820308	None:intergenic
AAAGCATCTTCATCGTTAGC+CGG	0.584931	3.4:+73820274	None:intergenic
AAATTCATGGTTGGTCATGT+CGG	0.587621	3.4:+73821185	None:intergenic
GGAGCGGAATGGGGTGAACA+AGG	0.588520	3.4:-73819909	MS.gene29082:CDS
ATTAACCTTTGATCCAGCAT+AGG	0.590030	3.4:+73821155	None:intergenic
GGAGTATTTACTGGCGATTG+TGG	0.593617	3.4:-73820017	MS.gene29082:CDS
ATCTTGGAAATCGAAGCTCT+CGG	0.596119	3.4:+73821389	None:intergenic
TACTTCAAGAACCAAAGCAA+TGG	0.600914	3.4:+73821413	None:intergenic
TGTGAGGAACTCATGGGGAG+CGG	0.601438	3.4:-73819925	MS.gene29082:CDS
TGTCCTCAGACAAAAGCTTG+TGG	0.605870	3.4:-73821359	MS.gene29082:CDS
ATGTTCCGAGGCACGCCACG+TGG	0.610728	3.4:-73821123	MS.gene29082:CDS
GCGTGCCTCGGAACATTCTG+TGG	0.611727	3.4:+73821130	None:intergenic
AGGAATGTATCTAACAAGGA+AGG	0.612463	3.4:-73819870	MS.gene29082:CDS
GAACTAATTATTGGATTGTG+AGG	0.613342	3.4:-73819941	MS.gene29082:CDS
GGTAGTCCTAAATTCATGGT+TGG	0.613532	3.4:+73821176	None:intergenic
AAAATCAAGGATGCAATGGT+GGG	0.617377	3.4:-73820535	MS.gene29082:CDS
ATAAGCTCTTGTTCAGACAA+AGG	0.634536	3.4:+73820575	None:intergenic
AAGGTTACATCAGAATGCAA+AGG	0.641547	3.4:-73819890	MS.gene29082:CDS
GCAAAGGAATGTATCTAACA+AGG	0.642601	3.4:-73819874	MS.gene29082:CDS
CTATGGCTACGGATACAGGT+TGG	0.643007	3.4:+73820233	None:intergenic
GGGACTTATACGAGAGATGG+AGG	0.652822	3.4:-73821338	MS.gene29082:CDS
GTATCCGTAGCCATAGATGC+CGG	0.655334	3.4:-73820226	MS.gene29082:CDS
AGGACCTTTATCATCTACCA+AGG	0.670444	3.4:-73819787	MS.gene29082:CDS
AATCACCACAGAATGTTCCG+AGG	0.693791	3.4:-73821135	MS.gene29082:CDS
TCCGTAGCCATAGATGCCGG+AGG	0.697215	3.4:-73820223	MS.gene29082:CDS
GTCAAGGATCAAGGCCAATG+TGG	0.699707	3.4:-73821021	MS.gene29082:intron
GAGATGGAGGAGTCACCACA+CGG	0.702988	3.4:-73821325	MS.gene29082:CDS
TGGATTGTGAGGAACTCATG+GGG	0.709984	3.4:-73819930	MS.gene29082:CDS
CAAGGTTCCGAGAAACCGTG+TGG	0.716333	3.4:+73821310	None:intergenic
GAAGAAAGGTGCTGTCACTG+AGG	0.722701	3.4:-73821043	MS.gene29082:CDS
ATGAATGTTCCACTTCCACG+TGG	0.756623	3.4:+73821108	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTACTTTTTTATTTTGA+AGG	+	chr3.4:73821105-73821124	None:intergenic	10.0%
!!	AGTGAATTGAATTTATAAAT+AGG	+	chr3.4:73820380-73820399	None:intergenic	15.0%
!!	TAAAAAAGTAAAAAAAGAGA+TGG	-	chr3.4:73821111-73821130	MS.gene29082:CDS	15.0%
!!	TAGTATAACTATTTGAAAAT+AGG	+	chr3.4:73820444-73820463	None:intergenic	15.0%
!!!	ATGAACAATTTTTGAATCTT+AGG	+	chr3.4:73820880-73820899	None:intergenic	20.0%
!	TAAACCAAATAAAGACAGAT+AGG	-	chr3.4:73820619-73820638	MS.gene29082:CDS	25.0%
!	TATAATTGACATGCAACTTT+AGG	-	chr3.4:73820852-73820871	MS.gene29082:intron	25.0%
!	TTGTTAAGTTTCAACTTGTA+AGG	+	chr3.4:73820013-73820032	None:intergenic	25.0%
!	TTTGAGTACATTAAGCAAAA+AGG	-	chr3.4:73820726-73820745	MS.gene29082:intron	25.0%
!!	ATTTTGTATACACCTATTAC+AGG	-	chr3.4:73821184-73821203	MS.gene29082:CDS	25.0%
!!	TATTGACTACATAAATGCAT+AGG	-	chr3.4:73820400-73820419	MS.gene29082:intron	25.0%
!!	TTGTGATTAATGTTTTGTAC+AGG	-	chr3.4:73820555-73820574	MS.gene29082:CDS	25.0%
!!	TTTTGTATACACCTATTACA+GGG	-	chr3.4:73821185-73821204	MS.gene29082:CDS	25.0%
!!!	CTTTTTTTAACTTACCACAT+TGG	+	chr3.4:73820219-73820238	None:intergenic	25.0%
	AAAGCTATAGTGCATAATAG+TGG	+	chr3.4:73820520-73820539	None:intergenic	30.0%
	CCAATAATTAGTTCCATCAA+CGG	+	chr3.4:73821276-73821295	None:intergenic	30.0%
	CTAACCTATCTGTCTTTATT+TGG	+	chr3.4:73820626-73820645	None:intergenic	30.0%
	GAACTAATTATTGGATTGTG+AGG	-	chr3.4:73821282-73821301	MS.gene29082:CDS	30.0%
	TCAAAAATTGTTCATGTTGC+AGG	-	chr3.4:73820885-73820904	MS.gene29082:intron	30.0%
	TGTGACAATCAAGAAAATCA+AGG	-	chr3.4:73820675-73820694	MS.gene29082:intron	30.0%
!	ATCTGATTTCCAGTTTTACT+CGG	-	chr3.4:73821023-73821042	MS.gene29082:CDS	30.0%
!!	TTTTGGAGGAGTTTTTAACT+GGG	+	chr3.4:73821409-73821428	None:intergenic	30.0%
!!	TTTTTGGAGGAGTTTTTAAC+TGG	+	chr3.4:73821410-73821429	None:intergenic	30.0%
	AAAATCAAGGATGCAATGGT+GGG	-	chr3.4:73820688-73820707	MS.gene29082:intron	35.0%
	AAGGTTACATCAGAATGCAA+AGG	-	chr3.4:73821333-73821352	MS.gene29082:CDS	35.0%
	AATCATGGTGTAGCAATAGT+AGG	-	chr3.4:73821239-73821258	MS.gene29082:CDS	35.0%
	ACATGACCAACCATGAATTT+AGG	-	chr3.4:73820041-73820060	MS.gene29082:intron	35.0%
	AGGAATGTATCTAACAAGGA+AGG	-	chr3.4:73821353-73821372	MS.gene29082:CDS	35.0%
	ATAAGCTCTTGTTCAGACAA+AGG	+	chr3.4:73820651-73820670	None:intergenic	35.0%
	ATTAACCTTTGATCCAGCAT+AGG	+	chr3.4:73820071-73820090	None:intergenic	35.0%
	ATTCTCTTGTGTCAATAGAG+AGG	+	chr3.4:73821069-73821088	None:intergenic	35.0%
	CAAGAAAATCAAGGATGCAA+TGG	-	chr3.4:73820684-73820703	MS.gene29082:intron	35.0%
	CAGTAAATACTCCCTGTAAT+AGG	+	chr3.4:73821199-73821218	None:intergenic	35.0%
	CATAGGTAGTCCTAAATTCA+TGG	+	chr3.4:73820054-73820073	None:intergenic	35.0%
	GAGTACATTAAGCAAAAAGG+TGG	-	chr3.4:73820729-73820748	MS.gene29082:intron	35.0%
	GCAAAGGAATGTATCTAACA+AGG	-	chr3.4:73821349-73821368	MS.gene29082:CDS	35.0%
	GTTGCAAACCTGAAACTTAA+AGG	-	chr3.4:73820336-73820355	MS.gene29082:intron	35.0%
	TACTTCAAGAACCAAAGCAA+TGG	+	chr3.4:73819813-73819832	None:intergenic	35.0%
	TCATCCTTGGTAGATGATAA+AGG	+	chr3.4:73821443-73821462	None:intergenic	35.0%
	TGTGGTAAAGAGCTAAATCA+TGG	-	chr3.4:73821224-73821243	MS.gene29082:CDS	35.0%
!	AAATTCATGGTTGGTCATGT+CGG	+	chr3.4:73820041-73820060	None:intergenic	35.0%
!	CCGTTGATGGAACTAATTAT+TGG	-	chr3.4:73821273-73821292	MS.gene29082:CDS	35.0%
!	GTAAGGCTTATCCATTTTGT+TGG	+	chr3.4:73819996-73820015	None:intergenic	35.0%
!	TTTTTACCTCCGAGTAAAAC+TGG	+	chr3.4:73821035-73821054	None:intergenic	35.0%
!!	ATCCATCATTTGCTGTGTAA+GGG	+	chr3.4:73820776-73820795	None:intergenic	35.0%
!!	CTATTACAGGGAGTATTTAC+TGG	-	chr3.4:73821197-73821216	MS.gene29082:CDS	35.0%
!!!	TATCCATTTTGTTGGTGTTG+TGG	+	chr3.4:73819988-73820007	None:intergenic	35.0%
	AAACTGGAAATCAGATCCTC+CGG	+	chr3.4:73821019-73821038	None:intergenic	40.0%
	AGGACCTTTATCATCTACCA+AGG	-	chr3.4:73821436-73821455	MS.gene29082:CDS	40.0%
	ATCTTGGAAATCGAAGCTCT+CGG	+	chr3.4:73819837-73819856	None:intergenic	40.0%
	ATTGGATTGTGAGGAACTCA+TGG	-	chr3.4:73821291-73821310	MS.gene29082:CDS	40.0%
	CTGAGGACACATCTTTATCT+TGG	+	chr3.4:73819853-73819872	None:intergenic	40.0%
	GAAAATCAAGGATGCAATGG+TGG	-	chr3.4:73820687-73820706	MS.gene29082:intron	40.0%
	GACACCATCTGATCAAAGAT+CGG	-	chr3.4:73820290-73820309	MS.gene29082:CDS	40.0%
	GACCATCAATTGATACAGCT+GGG	+	chr3.4:73820918-73820937	None:intergenic	40.0%
	GATTGTTCTGTCCATTGCTT+TGG	-	chr3.4:73819799-73819818	MS.gene29082:CDS	40.0%
	GGTAGTCCTAAATTCATGGT+TGG	+	chr3.4:73820050-73820069	None:intergenic	40.0%
	GGTGTAGCAATAGTAGGATA+TGG	-	chr3.4:73821245-73821264	MS.gene29082:CDS	40.0%
	TACCCTTACACAGCAAATGA+TGG	-	chr3.4:73820771-73820790	MS.gene29082:intron	40.0%
	TCAACTGTTGTAGCTGTTGA+AGG	-	chr3.4:73820594-73820613	MS.gene29082:CDS	40.0%
	TCATCCGATCTTTGATCAGA+TGG	+	chr3.4:73820297-73820316	None:intergenic	40.0%
	TCTAACAAGGAAGGACTTTG+TGG	-	chr3.4:73821362-73821381	MS.gene29082:CDS	40.0%
	TGACCATCAATTGATACAGC+TGG	+	chr3.4:73820919-73820938	None:intergenic	40.0%
	TGTCCACAACACCAACAAAA+TGG	-	chr3.4:73819982-73820001	MS.gene29082:CDS	40.0%
	TTGGATTGTGAGGAACTCAT+GGG	-	chr3.4:73821292-73821311	MS.gene29082:CDS	40.0%
!	AAAGCATCTTCATCGTTAGC+CGG	+	chr3.4:73820952-73820971	None:intergenic	40.0%
!	GAATTTAGGACTACCTATGC+TGG	-	chr3.4:73820055-73820074	MS.gene29082:intron	40.0%
!	GTTTTGTACAGGTAGTTGCT+GGG	-	chr3.4:73820566-73820585	MS.gene29082:CDS	40.0%
!	TAAAGGTCCTGCTGGATTTT+TGG	+	chr3.4:73821426-73821445	None:intergenic	40.0%
!	TCTGTTGATTGGAGGAAGAA+AGG	-	chr3.4:73820166-73820185	MS.gene29082:intron	40.0%
!	TGATTTCCAGTTTTACTCGG+AGG	-	chr3.4:73821026-73821045	MS.gene29082:CDS	40.0%
!	TGTTTTGTACAGGTAGTTGC+TGG	-	chr3.4:73820565-73820584	MS.gene29082:CDS	40.0%
!!	AGCGCTTTTGTTTCTCATCA+AGG	+	chr3.4:73819934-73819953	None:intergenic	40.0%
!!	GATCCATCATTTGCTGTGTA+AGG	+	chr3.4:73820777-73820796	None:intergenic	40.0%
	AACTCCTCCAAAAATCCAGC+AGG	-	chr3.4:73821416-73821435	MS.gene29082:CDS	45.0%
	AAGCTCCTGCTTCTGTTGAT+TGG	-	chr3.4:73820155-73820174	MS.gene29082:intron	45.0%
	AATCACCACAGAATGTTCCG+AGG	-	chr3.4:73820088-73820107	MS.gene29082:intron	45.0%
	AGGACTTTGTGGCATAGCTA+TGG	-	chr3.4:73821373-73821392	MS.gene29082:CDS	45.0%
	ATCAACAGAAGCAGGAGCTT+TGG	+	chr3.4:73820155-73820174	None:intergenic	45.0%
	ATGAATGTTCCACTTCCACG+TGG	+	chr3.4:73820118-73820137	None:intergenic	45.0%
	CCTGAAACTTAAAGGACTCG+TGG	-	chr3.4:73820344-73820363	MS.gene29082:intron	45.0%
	GACTACCTATGCTGGATCAA+AGG	-	chr3.4:73820063-73820082	MS.gene29082:intron	45.0%
	GGATATGGAACAACCGTTGA+TGG	-	chr3.4:73821260-73821279	MS.gene29082:CDS	45.0%
	GTAGATGATAAAGGTCCTGC+TGG	+	chr3.4:73821434-73821453	None:intergenic	45.0%
	GTCCCAGCTGTATCAATTGA+TGG	-	chr3.4:73820913-73820932	MS.gene29082:intron	45.0%
	GTCCTCAGACAAAAGCTTGT+GGG	-	chr3.4:73819865-73819884	MS.gene29082:CDS	45.0%
	TGATGGTCATGAGACTGTTC+CGG	-	chr3.4:73820930-73820949	MS.gene29082:intron	45.0%
	TGGATTGTGAGGAACTCATG+GGG	-	chr3.4:73821293-73821312	MS.gene29082:CDS	45.0%
	TGTCCTCAGACAAAAGCTTG+TGG	-	chr3.4:73819864-73819883	MS.gene29082:CDS	45.0%
	TGTGGGACTTATACGAGAGA+TGG	-	chr3.4:73819882-73819901	MS.gene29082:CDS	45.0%
	TTCCTCCAATCAACAGAAGC+AGG	+	chr3.4:73820163-73820182	None:intergenic	45.0%
!	CCACGAGTCCTTTAAGTTTC+AGG	+	chr3.4:73820347-73820366	None:intergenic	45.0%
!	GGAGTATTTACTGGCGATTG+TGG	-	chr3.4:73821206-73821225	MS.gene29082:CDS	45.0%
!!	GGAGGAGTTTTTAACTGGGT+AGG	+	chr3.4:73821405-73821424	None:intergenic	45.0%
!!	AAAAATATAATATATAACTT+AGG	+	chr3.4:73820497-73820516	None:intergenic	5.0%
	ATCAGATCCTCCGGCATCTA+TGG	+	chr3.4:73821010-73821029	None:intergenic	50.0%
	CTATGGCTACGGATACAGGT+TGG	+	chr3.4:73820993-73821012	None:intergenic	50.0%
	GCATCTATGGCTACGGATAC+AGG	+	chr3.4:73820997-73821016	None:intergenic	50.0%
	GGGACTTATACGAGAGATGG+AGG	-	chr3.4:73819885-73819904	MS.gene29082:CDS	50.0%
	GTATCCGTAGCCATAGATGC+CGG	-	chr3.4:73820997-73821016	MS.gene29082:intron	50.0%
	GTCAAGGATCAAGGCCAATG+TGG	-	chr3.4:73820202-73820221	MS.gene29082:CDS	50.0%
	GTCACTGAGGTCAAGGATCA+AGG	-	chr3.4:73820193-73820212	MS.gene29082:intron	50.0%
!	CTCCTGCTTCTGTTGATTGG+AGG	-	chr3.4:73820158-73820177	MS.gene29082:intron	50.0%
!	CTGTTGACTGCGTGAATGCA+TGG	+	chr3.4:73820267-73820286	None:intergenic	50.0%
!	GTCCCACAAGCTTTTGTCTG+AGG	+	chr3.4:73819870-73819889	None:intergenic	50.0%
!!	AGGATGCAATGGTGGGCTAA+TGG	-	chr3.4:73820695-73820714	MS.gene29082:intron	50.0%
!!	AGGTCCTGCTGGATTTTTGG+AGG	+	chr3.4:73821423-73821442	None:intergenic	50.0%
!!	GAAGAAAGGTGCTGTCACTG+AGG	-	chr3.4:73820180-73820199	MS.gene29082:intron	50.0%
	AACTCATGGGGAGCGGAATG+GGG	-	chr3.4:73821305-73821324	MS.gene29082:CDS	55.0%
	CAAGGTTCCGAGAAACCGTG+TGG	+	chr3.4:73819916-73819935	None:intergenic	55.0%
	GAACTCATGGGGAGCGGAAT+GGG	-	chr3.4:73821304-73821323	MS.gene29082:CDS	55.0%
	GAGATGGAGGAGTCACCACA+CGG	-	chr3.4:73819898-73819917	MS.gene29082:CDS	55.0%
	GGAGTCACCACACGGTTTCT+CGG	-	chr3.4:73819906-73819925	MS.gene29082:CDS	55.0%
	TCCTCCGGCATCTATGGCTA+CGG	+	chr3.4:73821004-73821023	None:intergenic	55.0%
	TGTGAGGAACTCATGGGGAG+CGG	-	chr3.4:73821298-73821317	MS.gene29082:CDS	55.0%
!	AGGTGCTGTCACTGAGGTCA+AGG	-	chr3.4:73820186-73820205	MS.gene29082:intron	55.0%
!!	GGCTACGGATACAGGTTGGT+TGG	+	chr3.4:73820989-73821008	None:intergenic	55.0%
	GCGTGCCTCGGAACATTCTG+TGG	+	chr3.4:73820096-73820115	None:intergenic	60.0%
	GGAACTCATGGGGAGCGGAA+TGG	-	chr3.4:73821303-73821322	MS.gene29082:CDS	60.0%
	GGAGCGGAATGGGGTGAACA+AGG	-	chr3.4:73821314-73821333	MS.gene29082:CDS	60.0%
	TCCGTAGCCATAGATGCCGG+AGG	-	chr3.4:73821000-73821019	MS.gene29082:intron	60.0%
	ATGTTCCGAGGCACGCCACG+TGG	-	chr3.4:73820100-73820119	MS.gene29082:intron	65.0%
	CACTTCCACGTGGCGTGCCT+CGG	+	chr3.4:73820108-73820127	None:intergenic	65.0%
	CGAGGCACGCCACGTGGAAG+TGG	-	chr3.4:73820106-73820125	MS.gene29082:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	73819776	73821469	73819776	ID=MS.gene29082
chr3.4	mRNA	73819776	73821469	73819776	ID=MS.gene29082.t1;Parent=MS.gene29082
chr3.4	exon	73821022	73821469	73821022	ID=MS.gene29082.t1.exon1;Parent=MS.gene29082.t1
chr3.4	CDS	73821022	73821469	73821022	ID=cds.MS.gene29082.t1;Parent=MS.gene29082.t1
chr3.4	exon	73820433	73820668	73820433	ID=MS.gene29082.t1.exon2;Parent=MS.gene29082.t1
chr3.4	CDS	73820433	73820668	73820433	ID=cds.MS.gene29082.t1;Parent=MS.gene29082.t1
chr3.4	exon	73820198	73820338	73820198	ID=MS.gene29082.t1.exon3;Parent=MS.gene29082.t1
chr3.4	CDS	73820198	73820338	73820198	ID=cds.MS.gene29082.t1;Parent=MS.gene29082.t1
chr3.4	exon	73819776	73820039	73819776	ID=MS.gene29082.t1.exon4;Parent=MS.gene29082.t1
chr3.4	CDS	73819776	73820039	73819776	ID=cds.MS.gene29082.t1;Parent=MS.gene29082.t1

Gene Sequence

>MS.gene29082

ATGGAAACTAAGAATCTATTGTTGATTGTTCTGTCCATTGCTTTGGTTCTTGAAGTATCCGAGAGCTTCGATTTCCAAGATAAAGATGTGTCCTCAGACAAAAGCTTGTGGGACTTATACGAGAGATGGAGGAGTCACCACACGGTTTCTCGGAACCTTGATGAGAAACAAAAGCGCTTTAACGTGTTCAAAACAAACGTGATGCATGTCCACAACACCAACAAAATGGATAAGCCTTACAAGTTGAAACTTAACAAGTTTGCCGACATGACCAACCATGAATTTAGGACTACCTATGCTGGATCAAAGGTTAATCACCACAGAATGTTCCGAGGCACGCCACGTGGAAGTGGAACATTCATGTATGAGAATTTCACCAAAGCTCCTGCTTCTGTTGATTGGAGGAAGAAAGGTGCTGTCACTGAGGTCAAGGATCAAGGCCAATGTGGTAAGTTAAAAAAAGTTACTATTTAGATTCTTTAACATCACCATGCATTCACGCAGTCAACAGTTTGACACCATCTGATCAAAGATCGGATGACTCTTGTTACGAGCAATCTGTTGCAAACCTGAAACTTAAAGGACTCGTGGTGTAATTTAGCCTATTTATAAATTCAATTCACTTATTGACTACATAAATGCATAGGATTTTTGACTGAAGTCAACCTATTTTCAAATAGTTATACTAGTCATTAATAATCATATTCATAGAGCTTAACCTAAGTTATATATTATATTTTTCCACTATTATGCACTATAGCTTTTTATAACATTGTTGTTTGTGATTAATGTTTTGTACAGGTAGTTGCTGGGCTTTTTCAACTGTTGTAGCTGTTGAAGGCATAAACCAAATAAAGACAGATAGGTTAGTGCCTTTGTCTGAACAAGAGCTTATAGATTGTGACAATCAAGAAAATCAAGGATGCAATGGTGGGCTAATGGAATATGCTTTTGAGTACATTAAGCAAAAAGGTGGCTTAACAACAGAAAGCTATTACCCTTACACAGCAAATGATGGATCATGTGATGCAACAAAAGTGAGTAAAAGATGCTAAACTTCTATTGAAAATTTTTAATATAATTGACATGCAACTTTAGGAACCTAAGATTCAAAAATTGTTCATGTTGCAGGAAAATGTCCCAGCTGTATCAATTGATGGTCATGAGACTGTTCCGGCTAACGATGAAGATGCTTTGCTCAAAGCTGTTGCCAACCAACCTGTATCCGTAGCCATAGATGCCGGAGGATCTGATTTCCAGTTTTACTCGGAGGTAAAAATGAGAATGAATCCTCTCTATTGACACAAGAGAATAATGCAATCTAAACCTTCAAAATAAAAAAGTAAAAAAAGAGATGGAAGAAATATGCTATTATTAGCATTCAATTTTGAAACTAATGTGTATCTTAATTTTGTATACACCTATTACAGGGAGTATTTACTGGCGATTGTGGTAAAGAGCTAAATCATGGTGTAGCAATAGTAGGATATGGAACAACCGTTGATGGAACTAATTATTGGATTGTGAGGAACTCATGGGGAGCGGAATGGGGTGAACAAGGTTACATCAGAATGCAAAGGAATGTATCTAACAAGGAAGGACTTTGTGGCATAGCTATGGAAGCTTCCTACCCAGTTAAAAACTCCTCCAAAAATCCAGCAGGACCTTTATCATCTACCAAGGATGAGCTTTGA

Protein sequence

>MS.gene29082.t1

METKNLLLIVLSIALVLEVSESFDFQDKDVSSDKSLWDLYERWRSHHTVSRNLDEKQKRFNVFKTNVMHVHNTNKMDKPYKLKLNKFADMTNHEFRTTYAGSKVNHHRMFRGTPRGSGTFMYENFTKAPASVDWRKKGAVTEVKDQGQCGSCWAFSTVVAVEGINQIKTDRLVPLSEQELIDCDNQENQGCNGGLMEYAFEYIKQKGGLTTESYYPYTANDGSCDATKENVPAVSIDGHETVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCGKELNHGVAIVGYGTTVDGTNYWIVRNSWGAEWGEQGYIRMQRNVSNKEGLCGIAMEASYPVKNSSKNPAGPLSSTKDEL