AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033324

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033324.t1 MTR_4g104370 96.944 360 11 0 1 360 1 360 0.0 737
MS.gene033324.t1 MTR_3g075390 74.033 362 92 2 1 360 1 362 0.0 578
MS.gene033324.t1 MTR_4g079770 54.598 348 147 9 1 342 1 343 7.10e-127 368
MS.gene033324.t1 MTR_4g107930 53.890 347 151 7 1 342 1 343 4.20e-126 366
MS.gene033324.t1 MTR_4g079470 57.547 318 125 8 30 342 31 343 3.97e-125 363
MS.gene033324.t1 MTR_5g022560 53.757 346 143 9 5 342 6 342 5.10e-125 363
MS.gene033324.t1 MTR_4g047610 58.786 313 117 8 38 342 33 341 1.42e-124 362
MS.gene033324.t1 MTR_4g079800 57.233 318 126 8 30 342 31 343 1.79e-124 362
MS.gene033324.t1 MTR_3g116080 53.736 348 149 7 5 345 7 349 1.66e-123 360
MS.gene033324.t1 MTR_4g080730 56.782 317 125 8 30 342 30 338 1.68e-122 357
MS.gene033324.t1 MTR_4g080700 57.692 312 120 8 35 342 35 338 1.72e-122 357
MS.gene033324.t1 MTR_1g107550 54.740 327 142 6 23 345 33 357 1.92e-119 353
MS.gene033324.t1 MTR_2g075830 54.070 344 142 9 5 342 6 339 1.74e-118 347
MS.gene033324.t1 MTR_1g018840 50.423 355 163 8 1 344 10 362 1.67e-117 348
MS.gene033324.t1 MTR_1g018840 50.423 355 163 8 1 344 10 362 5.20e-117 347
MS.gene033324.t1 MTR_4g094918 49.713 348 162 7 1 344 4 342 8.50e-117 343
MS.gene033324.t1 MTR_4g079440 55.375 307 126 6 40 343 46 344 4.23e-116 340
MS.gene033324.t1 MTR_3g102220 50.430 349 162 8 6 345 17 363 7.30e-114 339
MS.gene033324.t1 MTR_4g080360 49.682 314 153 4 33 343 30 341 7.42e-109 322
MS.gene033324.t1 MTR_2g090890 51.948 308 136 6 40 344 39 337 3.19e-108 320
MS.gene033324.t1 MTR_2g083930 48.378 339 161 7 11 343 13 343 1.55e-106 316
MS.gene033324.t1 MTR_7g078600 50.464 323 140 10 26 343 30 337 3.50e-103 308
MS.gene033324.t1 MTR_3g112430 48.875 311 155 4 39 346 35 344 1.87e-96 294
MS.gene033324.t1 MTR_7g079230 47.605 334 158 8 13 342 14 334 2.00e-96 290
MS.gene033324.t1 MTR_2g015850 46.465 297 154 4 48 343 71 363 2.24e-84 260
MS.gene033324.t1 MTR_2g016180 42.857 350 180 12 8 345 16 357 7.92e-84 263
MS.gene033324.t1 MTR_2g016270 42.286 350 167 12 8 345 16 342 4.69e-83 260
MS.gene033324.t1 MTR_4g065077 40.223 358 181 15 6 344 14 357 2.88e-70 224
MS.gene033324.t1 MTR_2g067900 41.317 334 175 12 22 343 2 326 1.59e-68 218
MS.gene033324.t1 MTR_2g015920 37.353 340 187 13 30 355 43 370 1.21e-66 215
MS.gene033324.t1 MTR_4g125300 42.904 303 144 13 52 343 64 348 6.32e-65 210
MS.gene033324.t1 MTR_8g086470 39.377 353 175 14 6 344 30 357 6.39e-65 210
MS.gene033324.t1 MTR_2g067560 42.271 317 155 14 40 337 16 323 6.90e-65 221
MS.gene033324.t1 MTR_2g015930 38.182 330 177 14 30 344 42 359 1.41e-61 201
MS.gene033324.t1 MTR_1g023210 38.742 302 150 12 54 335 66 352 2.58e-58 193
MS.gene033324.t1 MTR_2g064475 35.673 342 171 13 40 341 16 348 3.00e-56 187
MS.gene033324.t1 MTR_1g023810 41.593 226 123 6 120 341 41 261 1.18e-55 183
MS.gene033324.t1 MTR_4g071060 37.049 305 160 10 49 335 58 348 1.80e-54 183
MS.gene033324.t1 MTR_2g066980 35.202 321 153 11 40 342 34 317 2.27e-54 181
MS.gene033324.t1 MTR_7g078630 39.604 303 132 12 19 306 687 953 2.38e-53 189
MS.gene033324.t1 MTR_1g094135 33.728 338 194 10 15 335 34 358 2.48e-53 181
MS.gene033324.t1 MTR_7g078580 65.657 99 33 1 245 343 4 101 1.13e-42 145
MS.gene033324.t1 MTR_4g072790 36.644 292 143 13 49 335 58 312 2.46e-40 145
MS.gene033324.t1 MTR_7g079130 67.033 91 30 0 253 343 7 97 1.90e-39 135
MS.gene033324.t1 MTR_4g072340 34.237 295 150 11 49 335 58 316 1.01e-37 138
MS.gene033324.t1 MTR_4g073660 34.797 296 147 13 49 335 57 315 1.50e-37 137
MS.gene033324.t1 MTR_1g025450 40.556 180 82 7 124 298 51 210 1.79e-34 133
MS.gene033324.t1 MTR_2g015840 50.450 111 54 1 232 342 161 270 4.65e-33 124
MS.gene033324.t1 MTR_2g015840 35.256 156 94 5 11 163 5 156 3.37e-20 89.4
MS.gene033324.t1 MTR_2g016380 43.902 123 62 4 227 345 18 137 5.45e-27 107
MS.gene033324.t1 MTR_2g047960 65.385 78 26 1 138 214 8 85 1.40e-25 99.4
MS.gene033324.t1 MTR_3g029560 32.075 212 114 6 139 343 49 237 1.79e-24 100
MS.gene033324.t1 MTR_7g111060 26.906 223 137 7 134 335 113 330 1.41e-21 94.7
MS.gene033324.t1 MTR_7g111050 29.630 243 143 8 126 345 102 339 1.87e-21 94.4
MS.gene033324.t1 MTR_7g079260 56.944 72 15 1 269 340 7 62 2.72e-21 88.2
MS.gene033324.t1 MTR_7g078310 38.356 146 75 5 75 216 110 244 2.85e-19 86.7
MS.gene033324.t1 MTR_7g111050 28.713 202 122 6 126 306 97 297 1.19e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033324.t1 AT5G50260 69.529 361 107 2 3 360 1 361 0.0 536
MS.gene033324.t1 AT3G48340 64.917 362 122 4 3 360 1 361 3.60e-172 483
MS.gene033324.t1 AT3G48350 62.500 360 133 2 3 360 1 360 4.53e-168 473
MS.gene033324.t1 AT3G48350 61.733 277 104 2 3 277 1 277 1.51e-122 355
MS.gene033324.t1 AT1G20850 53.416 322 145 4 25 344 36 354 3.88e-119 348
MS.gene033324.t1 AT4G35350 50.291 344 162 5 6 344 14 353 1.11e-117 345
MS.gene033324.t1 AT5G45890 49.292 353 152 9 3 342 6 344 1.36e-117 344
MS.gene033324.t1 AT3G19390 50.282 354 161 8 1 349 8 351 2.55e-116 345
MS.gene033324.t1 AT5G43060 51.934 362 150 9 1 345 1 355 1.11e-115 343
MS.gene033324.t1 AT1G47128 52.997 317 140 4 32 344 42 353 1.42e-112 336
MS.gene033324.t1 AT1G06260 48.680 341 165 6 7 344 10 343 2.51e-111 328
MS.gene033324.t1 AT4G36880 49.245 331 156 7 32 352 41 369 7.33e-110 326
MS.gene033324.t1 AT3G19400 47.536 345 158 10 18 356 26 353 1.48e-102 306
MS.gene033324.t1 AT2G27420 49.688 320 152 6 33 343 28 347 1.37e-101 304
MS.gene033324.t1 AT3G49340 47.785 316 157 6 33 343 28 340 1.90e-99 298
MS.gene033324.t1 AT4G11310 44.693 358 176 11 4 346 5 355 3.68e-99 298
MS.gene033324.t1 AT4G11320 48.243 313 152 8 39 346 55 362 1.46e-98 297
MS.gene033324.t1 AT4G11320 48.243 313 152 8 39 346 55 362 1.46e-98 297
MS.gene033324.t1 AT2G34080 44.571 350 172 7 6 343 5 344 3.85e-98 295
MS.gene033324.t1 AT4G23520 46.519 316 158 8 39 349 46 355 4.30e-97 293
MS.gene033324.t1 AT1G09850 48.065 310 153 6 38 344 30 334 9.77e-94 286
MS.gene033324.t1 AT1G29090 43.966 348 187 6 2 343 9 354 8.52e-93 281
MS.gene033324.t1 AT1G29080 43.925 321 169 7 30 343 29 345 1.47e-88 270
MS.gene033324.t1 AT4G35350 48.201 278 133 5 7 277 13 286 4.14e-84 257
MS.gene033324.t1 AT3G43960 42.151 344 179 11 13 347 18 350 2.51e-78 245
MS.gene033324.t1 AT1G29110 38.551 345 186 10 7 343 7 333 3.17e-74 233
MS.gene033324.t1 AT3G19400 46.360 261 126 8 18 272 26 278 8.57e-69 218
MS.gene033324.t1 AT5G60360 41.667 300 154 11 51 343 71 356 3.63e-64 208
MS.gene033324.t1 AT3G45310 41.333 300 155 11 51 343 71 356 2.44e-62 203
MS.gene033324.t1 AT5G60360 41.333 300 154 12 51 343 71 355 1.42e-61 201
MS.gene033324.t1 AT3G45310 41.000 300 155 12 51 343 71 355 9.36e-60 197
MS.gene033324.t1 AT5G60360 41.786 280 146 10 51 323 71 340 2.29e-59 196
MS.gene033324.t1 AT4G39090 38.511 309 161 9 44 335 56 352 5.82e-58 192
MS.gene033324.t1 AT2G21430 34.857 350 195 9 6 335 13 349 1.14e-57 191
MS.gene033324.t1 AT4G16190 36.364 297 164 8 55 335 71 358 5.90e-52 177
MS.gene033324.t1 AT3G54940 35.667 300 164 10 54 335 66 354 6.56e-50 171
MS.gene033324.t1 AT3G54940 37.811 201 110 6 54 244 66 261 2.84e-35 130
MS.gene033324.t1 AT1G02305 28.947 304 174 13 85 360 68 357 1.66e-25 105
MS.gene033324.t1 AT2G22160 50.000 96 36 2 60 151 15 102 3.38e-21 87.8
MS.gene033324.t1 AT4G01610 26.241 282 167 11 85 338 65 333 4.21e-21 93.6
MS.gene033324.t1 AT1G02300 29.114 237 137 8 147 360 146 374 5.98e-21 93.2
MS.gene033324.t1 AT4G01610 25.532 282 169 11 85 338 65 333 3.48e-19 88.2

Find 84 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGGCTTATCTAATTTAT+TGG 0.217778 8.2:-20331926 None:intergenic
TTATTCAACTTAAGCTTATA+AGG 0.250519 8.2:-20331943 None:intergenic
AGCTAAGAGCTTTGATTTCC+AGG 0.263284 8.2:+20331765 MS.gene033324:CDS
AGGATGCAACGGTGGGTTAA+TGG 0.279883 8.2:+20332541 MS.gene033324:CDS
TAACTTGTATAGGTAGTTGC+TGG 0.293976 8.2:+20332411 MS.gene033324:intron
GCATCAATAGCTACAGAAAT+AGG 0.294697 8.2:-20332864 None:intergenic
ATCATTTAAAGCTCATCTTT+TGG 0.331162 8.2:-20333261 None:intergenic
CTCAAGATGGAACAAAATAT+TGG 0.333579 8.2:+20333085 MS.gene033324:CDS
AAGCATCTATGCTGATTCTA+AGG 0.335794 8.2:+20331996 MS.gene033324:CDS
GGGAAGTGAATGGGGAGAAC+AGG 0.351413 8.2:+20333125 MS.gene033324:CDS
CAACAATGATGCTTCATTGT+TGG 0.354576 8.2:-20332823 None:intergenic
TAATTACAGGGAGTATTTAC+TGG 0.363002 8.2:+20333009 MS.gene033324:intron
TCGATTGATTGGAGGAAGAT+AGG 0.372632 8.2:+20332099 MS.gene033324:CDS
TTCGAGTTCATCAAGCAAAA+TGG 0.391365 8.2:+20332572 MS.gene033324:CDS
TCAACAATTGTAGCTGTTGA+AGG 0.396428 8.2:+20332440 MS.gene033324:CDS
ATGTTCCTTCTTCGATTGAT+TGG 0.402034 8.2:+20332088 MS.gene033324:CDS
ATTGGACAGTGAAGAACTCT+TGG 0.427329 8.2:+20333103 MS.gene033324:CDS
GAACTCTTGGGGAAGTGAAT+GGG 0.438030 8.2:+20333116 MS.gene033324:CDS
TATCTAATTTATTGGTATTG+TGG 0.443235 8.2:-20331918 None:intergenic
AAGCCAGCAGTGTCAATTGA+TGG 0.448320 8.2:+20332783 MS.gene033324:CDS
GGGGTAGCAATTGTGGGGTA+TGG 0.459702 8.2:+20333057 MS.gene033324:CDS
AATGATGCTTCATTGTTGGC+AGG 0.460490 8.2:-20332819 None:intergenic
TAAAATCAAAGCTAGGGATA+AGG 0.466804 8.2:-20331737 None:intergenic
TGGCCATCAATTGACACTGC+TGG 0.466959 8.2:-20332786 None:intergenic
AGGTGCTGTCACCGGGGTAA+AGG 0.468205 8.2:+20332119 MS.gene033324:CDS
GGAAGATAGGTGCTGTCACC+GGG 0.470663 8.2:+20332112 MS.gene033324:CDS
GGCATCAGAGAAAAGTTTGT+GGG 0.474251 8.2:+20331798 MS.gene033324:CDS
GAATCATGGGGTAGCAATTG+TGG 0.479525 8.2:+20333050 MS.gene033324:CDS
TTGGACAGTGAAGAACTCTT+GGG 0.479993 8.2:+20333104 MS.gene033324:CDS
AATCATGGGGTAGCAATTGT+GGG 0.482862 8.2:+20333051 MS.gene033324:CDS
AGAACTCTTGGGGAAGTGAA+TGG 0.483270 8.2:+20333115 MS.gene033324:CDS
TTATCCTTATGCTGCAAAAG+AGG 0.485453 8.2:+20332613 MS.gene033324:CDS
TGTGGGATTTGTACGAGAGA+TGG 0.486184 8.2:+20331815 MS.gene033324:CDS
AACTTGTATAGGTAGTTGCT+GGG 0.493124 8.2:+20332412 MS.gene033324:intron
CTATTCCTAAAATCAAAGCT+AGG 0.494841 8.2:-20331744 None:intergenic
AGCTACAGAAATAGGTTGGT+TGG 0.500901 8.2:-20332856 None:intergenic
GGAAGTGAATGGGGAGAACA+GGG 0.501956 8.2:+20333126 MS.gene033324:CDS
CTGATGCCAAGTCATTCTCC+TGG 0.502274 8.2:-20331783 None:intergenic
AGGTCTATGTGGCATAGCTA+TGG 0.507498 8.2:+20333185 MS.gene033324:CDS
TTCCTTCTTCGATTGATTGG+AGG 0.508300 8.2:+20332091 MS.gene033324:CDS
TATCCTTATGCTGCAAAAGA+GGG 0.511600 8.2:+20332614 MS.gene033324:CDS
TCTGTAGCTATTGATGCTGG+TGG 0.512891 8.2:+20332870 MS.gene033324:CDS
ATTTCTGTAGCTATTGATGC+TGG 0.514909 8.2:+20332867 MS.gene033324:CDS
TGCGACACTGCAGTGAATCA+AGG 0.524570 8.2:+20332521 MS.gene033324:CDS
AGGAAGATAGGTGCTGTCAC+CGG 0.527243 8.2:+20332111 MS.gene033324:CDS
TGTGGTACTGAGCTGAATCA+TGG 0.531881 8.2:+20333036 MS.gene033324:CDS
AACAAGTAAGCTTACCGCAT+TGG 0.533561 8.2:-20332149 None:intergenic
TTCCTCCAATCAATCGAAGA+AGG 0.537545 8.2:-20332093 None:intergenic
TATTCCTAAAATCAAAGCTA+GGG 0.538115 8.2:-20331743 None:intergenic
GGGAACCTGTAATACACAAA+AGG 0.540008 8.2:+20332634 MS.gene033324:CDS
GGGTTACATTAGAATGCAAA+GGG 0.552847 8.2:+20333146 MS.gene033324:CDS
GGGTATGGAGCAACTCAAGA+TGG 0.553677 8.2:+20333072 MS.gene033324:CDS
CACAAGTTCTTGCTCAGACA+AGG 0.554554 8.2:-20332495 None:intergenic
GTCACCGGGGTAAAGGATCA+AGG 0.557892 8.2:+20332126 MS.gene033324:CDS
TGATTTCCAGGAGAATGACT+TGG 0.561295 8.2:+20331777 MS.gene033324:CDS
TGTTGGCAGGGACATTCTCA+TGG 0.563794 8.2:-20332806 None:intergenic
CTTGTCTGAGCAAGAACTTG+TGG 0.566708 8.2:+20332496 MS.gene033324:CDS
AACTCTTGGGGAAGTGAATG+GGG 0.569712 8.2:+20333117 MS.gene033324:CDS
TGGCATCAGAGAAAAGTTTG+TGG 0.572881 8.2:+20331797 MS.gene033324:CDS
ATGATGCTTCATTGTTGGCA+GGG 0.574434 8.2:-20332818 None:intergenic
GTGGTACTGAGCTGAATCAT+GGG 0.579344 8.2:+20333037 MS.gene033324:CDS
CAATAGCTACAGAAATAGGT+TGG 0.582579 8.2:-20332860 None:intergenic
CATTGTCATGTGACATGCCA+CGG 0.595325 8.2:-20332038 None:intergenic
AGGGTTACATTAGAATGCAA+AGG 0.596326 8.2:+20333145 MS.gene033324:CDS
TCTATGTGGCATAGCTATGG+AGG 0.601740 8.2:+20333188 MS.gene033324:CDS
GTGAATCAAGGATGCAACGG+TGG 0.601861 8.2:+20332533 MS.gene033324:CDS
CGTGGCATGTCACATGACAA+TGG 0.606637 8.2:+20332039 MS.gene033324:CDS
TCTCACAAGCAAGGTCTATG+TGG 0.620594 8.2:+20333174 MS.gene033324:CDS
TGAATCAAGGATGCAACGGT+GGG 0.634962 8.2:+20332534 MS.gene033324:CDS
TTGGCCTTGATCCTTTACCC+CGG 0.635267 8.2:-20332130 None:intergenic
TCCACATTCTCATACATGAA+CGG 0.636776 8.2:-20332063 None:intergenic
ACCGTTCATGTATGAGAATG+TGG 0.645392 8.2:+20332062 MS.gene033324:CDS
GCAGTGAATCAAGGATGCAA+CGG 0.651301 8.2:+20332530 MS.gene033324:CDS
TGGACAGTGAAGAACTCTTG+GGG 0.663115 8.2:+20333105 MS.gene033324:CDS
ATTGTGGACATGCATAACAT+TGG 0.668124 8.2:-20331902 None:intergenic
AGGGACATATCTCACAAGCA+AGG 0.668337 8.2:+20333165 MS.gene033324:CDS
AATCATCATAGAATGTTCCG+TGG 0.676558 8.2:+20332021 MS.gene033324:CDS
GGAGTATTTACTGGACATTG+TGG 0.681946 8.2:+20333018 MS.gene033324:CDS
ATCATGGGGTAGCAATTGTG+GGG 0.693047 8.2:+20333052 MS.gene033324:CDS
CAAGACTTCGAGTAACTGTG+TGG 0.728098 8.2:-20331846 None:intergenic
GAAGATAGGTGCTGTCACCG+GGG 0.757764 8.2:+20332113 MS.gene033324:CDS
TGGTACTGAGCTGAATCATG+GGG 0.760193 8.2:+20333038 MS.gene033324:CDS
GTTCATGTATGAGAATGTGG+AGG 0.769545 8.2:+20332065 MS.gene033324:CDS
GTAAAGGATCAAGGCCAATG+CGG 0.813840 8.2:+20332135 MS.gene033324:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATATATAAATGTGTAA+GGG - chr8.2:20332942-20332961 None:intergenic 10.0%
!! AATTACAAAAATATAATTAC+AGG + chr8.2:20332996-20333015 MS.gene033324:intron 10.0%
!! ATTACAAAAATATAATTACA+GGG + chr8.2:20332997-20333016 MS.gene033324:intron 10.0%
!!! ATATTTTTGTAATTAAAAGT+GGG - chr8.2:20332990-20333009 None:intergenic 10.0%
!!! TATATTTTTGTAATTAAAAG+TGG - chr8.2:20332991-20333010 None:intergenic 10.0%
!! AAAATGTGTTAATACTTAAT+AGG - chr8.2:20332745-20332764 None:intergenic 15.0%
!! CAAAAATATATAAATGTGTA+AGG - chr8.2:20332943-20332962 None:intergenic 15.0%
!! ATAAGGCTTATCTAATTTAT+TGG - chr8.2:20331929-20331948 None:intergenic 20.0%
!! TATCTAATTTATTGGTATTG+TGG - chr8.2:20331921-20331940 None:intergenic 20.0%
!! TTATTCAACTTAAGCTTATA+AGG - chr8.2:20331946-20331965 None:intergenic 20.0%
! ATAATCAAATACAGACTAAG+TGG - chr8.2:20332288-20332307 None:intergenic 25.0%
! TATTCCTAAAATCAAAGCTA+GGG - chr8.2:20331746-20331765 None:intergenic 25.0%
! TCTGTATTTGATTATACGTT+TGG + chr8.2:20332293-20332312 MS.gene033324:intron 25.0%
!! TTTTCAGATGACTTTTTGAT+TGG - chr8.2:20333222-20333241 None:intergenic 25.0%
!!! GAATTGTTTCTAACTTGTAT+AGG + chr8.2:20332401-20332420 MS.gene033324:intron 25.0%
CTATTCCTAAAATCAAAGCT+AGG - chr8.2:20331747-20331766 None:intergenic 30.0%
CTCAAGATGGAACAAAATAT+TGG + chr8.2:20333085-20333104 MS.gene033324:CDS 30.0%
TAAAATCAAAGCTAGGGATA+AGG - chr8.2:20331740-20331759 None:intergenic 30.0%
TGTAATTAAAAGTGGGATCA+AGG - chr8.2:20332983-20333002 None:intergenic 30.0%
TTCAAGCGATTTAACTAACA+TGG - chr8.2:20332242-20332261 None:intergenic 30.0%
! AACACATTTTCACTTGATGT+AGG + chr8.2:20332755-20332774 MS.gene033324:intron 30.0%
! GTTTTACCTCTGAATAAAAC+TGG - chr8.2:20332905-20332924 None:intergenic 30.0%
! TTGGAAGTGAACTTTTTGTT+GGG - chr8.2:20333245-20333264 None:intergenic 30.0%
! TTTGGAAGTGAACTTTTTGT+TGG - chr8.2:20333246-20333265 None:intergenic 30.0%
!! AGATGACTTTTTGATTGGAT+AGG - chr8.2:20333217-20333236 None:intergenic 30.0%
!! CTAGTTTTTGTGTCTTGATT+TGG - chr8.2:20332472-20332491 None:intergenic 30.0%
!! GTTCTGAAGTAGAATTGATT+TGG + chr8.2:20332372-20332391 MS.gene033324:intron 30.0%
!! TAATTACAGGGAGTATTTAC+TGG + chr8.2:20333009-20333028 MS.gene033324:intron 30.0%
!!! TGATTTCCAGTTTTATTCAG+AGG + chr8.2:20332896-20332915 MS.gene033324:CDS 30.0%
!!! TTATCCCTAGCTTTGATTTT+AGG + chr8.2:20331739-20331758 MS.gene033324:CDS 30.0%
AACTTGTATAGGTAGTTGCT+GGG + chr8.2:20332412-20332431 MS.gene033324:intron 35.0%
AATCATCATAGAATGTTCCG+TGG + chr8.2:20332021-20332040 MS.gene033324:CDS 35.0%
AGGGTTACATTAGAATGCAA+AGG + chr8.2:20333145-20333164 MS.gene033324:CDS 35.0%
ATGTTCCTTCTTCGATTGAT+TGG + chr8.2:20332088-20332107 MS.gene033324:CDS 35.0%
ATTGTGGACATGCATAACAT+TGG - chr8.2:20331905-20331924 None:intergenic 35.0%
ATTTCTGTAGCTATTGATGC+TGG + chr8.2:20332867-20332886 MS.gene033324:CDS 35.0%
CAACAATGATGCTTCATTGT+TGG - chr8.2:20332826-20332845 None:intergenic 35.0%
CAATAGCTACAGAAATAGGT+TGG - chr8.2:20332863-20332882 None:intergenic 35.0%
GCATCAATAGCTACAGAAAT+AGG - chr8.2:20332867-20332886 None:intergenic 35.0%
GGGTTACATTAGAATGCAAA+GGG + chr8.2:20333146-20333165 MS.gene033324:CDS 35.0%
GTTTGGATCCAAAATTGATC+TGG + chr8.2:20332310-20332329 MS.gene033324:intron 35.0%
TATCCTTATGCTGCAAAAGA+GGG + chr8.2:20332614-20332633 MS.gene033324:CDS 35.0%
TCAACAATTGTAGCTGTTGA+AGG + chr8.2:20332440-20332459 MS.gene033324:CDS 35.0%
TCCACATTCTCATACATGAA+CGG - chr8.2:20332066-20332085 None:intergenic 35.0%
TTATCCTTATGCTGCAAAAG+AGG + chr8.2:20332613-20332632 MS.gene033324:CDS 35.0%
! TAACTTGTATAGGTAGTTGC+TGG + chr8.2:20332411-20332430 MS.gene033324:intron 35.0%
! TTCGAGTTCATCAAGCAAAA+TGG + chr8.2:20332572-20332591 MS.gene033324:CDS 35.0%
!! AAGCATCTATGCTGATTCTA+AGG + chr8.2:20331996-20332015 MS.gene033324:CDS 35.0%
AACAAGTAAGCTTACCGCAT+TGG - chr8.2:20332152-20332171 None:intergenic 40.0%
AATCATGGGGTAGCAATTGT+GGG + chr8.2:20333051-20333070 MS.gene033324:CDS 40.0%
AATGATGCTTCATTGTTGGC+AGG - chr8.2:20332822-20332841 None:intergenic 40.0%
ACCGTTCATGTATGAGAATG+TGG + chr8.2:20332062-20332081 MS.gene033324:CDS 40.0%
ATGATGCTTCATTGTTGGCA+GGG - chr8.2:20332821-20332840 None:intergenic 40.0%
ATTGGACAGTGAAGAACTCT+TGG + chr8.2:20333103-20333122 MS.gene033324:CDS 40.0%
CCAAAATTGATCTGGAGAAC+TGG + chr8.2:20332318-20332337 MS.gene033324:intron 40.0%
GGCATCAGAGAAAAGTTTGT+GGG + chr8.2:20331798-20331817 MS.gene033324:CDS 40.0%
GGGAACCTGTAATACACAAA+AGG + chr8.2:20332634-20332653 MS.gene033324:CDS 40.0%
GTTCATGTATGAGAATGTGG+AGG + chr8.2:20332065-20332084 MS.gene033324:CDS 40.0%
TCGATTGATTGGAGGAAGAT+AGG + chr8.2:20332099-20332118 MS.gene033324:CDS 40.0%
TGATTTCCAGGAGAATGACT+TGG + chr8.2:20331777-20331796 MS.gene033324:CDS 40.0%
TGGCATCAGAGAAAAGTTTG+TGG + chr8.2:20331797-20331816 MS.gene033324:CDS 40.0%
TTCCTCCAATCAATCGAAGA+AGG - chr8.2:20332096-20332115 None:intergenic 40.0%
TTCCTTCTTCGATTGATTGG+AGG + chr8.2:20332091-20332110 MS.gene033324:CDS 40.0%
TTGGACAGTGAAGAACTCTT+GGG + chr8.2:20333104-20333123 MS.gene033324:CDS 40.0%
! AGCTAAGAGCTTTGATTTCC+AGG + chr8.2:20331765-20331784 MS.gene033324:CDS 40.0%
! CCAGTTCTCCAGATCAATTT+TGG - chr8.2:20332321-20332340 None:intergenic 40.0%
! GGAGTATTTACTGGACATTG+TGG + chr8.2:20333018-20333037 MS.gene033324:CDS 40.0%
! GTGCACCTTTTGTGTATTAC+AGG - chr8.2:20332642-20332661 None:intergenic 40.0%
!! AGCTACAGAAATAGGTTGGT+TGG - chr8.2:20332859-20332878 None:intergenic 40.0%
AACTCTTGGGGAAGTGAATG+GGG + chr8.2:20333117-20333136 MS.gene033324:CDS 45.0%
AAGCCAGCAGTGTCAATTGA+TGG + chr8.2:20332783-20332802 MS.gene033324:CDS 45.0%
AGAACTCTTGGGGAAGTGAA+TGG + chr8.2:20333115-20333134 MS.gene033324:CDS 45.0%
AGGGACATATCTCACAAGCA+AGG + chr8.2:20333165-20333184 MS.gene033324:CDS 45.0%
AGGTCTATGTGGCATAGCTA+TGG + chr8.2:20333185-20333204 MS.gene033324:CDS 45.0%
ATCATGGGGTAGCAATTGTG+GGG + chr8.2:20333052-20333071 MS.gene033324:CDS 45.0%
CAAGACTTCGAGTAACTGTG+TGG - chr8.2:20331849-20331868 None:intergenic 45.0%
CACAAGTTCTTGCTCAGACA+AGG - chr8.2:20332498-20332517 None:intergenic 45.0%
CATTGTCATGTGACATGCCA+CGG - chr8.2:20332041-20332060 None:intergenic 45.0%
CTTGTCTGAGCAAGAACTTG+TGG + chr8.2:20332496-20332515 MS.gene033324:CDS 45.0%
GAACTCTTGGGGAAGTGAAT+GGG + chr8.2:20333116-20333135 MS.gene033324:CDS 45.0%
GAATCATGGGGTAGCAATTG+TGG + chr8.2:20333050-20333069 MS.gene033324:CDS 45.0%
GCAGTGAATCAAGGATGCAA+CGG + chr8.2:20332530-20332549 MS.gene033324:CDS 45.0%
GTAAAGGATCAAGGCCAATG+CGG + chr8.2:20332135-20332154 MS.gene033324:CDS 45.0%
TCTATGTGGCATAGCTATGG+AGG + chr8.2:20333188-20333207 MS.gene033324:CDS 45.0%
TCTCACAAGCAAGGTCTATG+TGG + chr8.2:20333174-20333193 MS.gene033324:CDS 45.0%
TCTGTAGCTATTGATGCTGG+TGG + chr8.2:20332870-20332889 MS.gene033324:CDS 45.0%
TGAATCAAGGATGCAACGGT+GGG + chr8.2:20332534-20332553 MS.gene033324:CDS 45.0%
TGGACAGTGAAGAACTCTTG+GGG + chr8.2:20333105-20333124 MS.gene033324:CDS 45.0%
TGTGGGATTTGTACGAGAGA+TGG + chr8.2:20331815-20331834 MS.gene033324:CDS 45.0%
! GTGGTACTGAGCTGAATCAT+GGG + chr8.2:20333037-20333056 MS.gene033324:CDS 45.0%
! GTTCCCTCTTTTGCAGCATA+AGG - chr8.2:20332620-20332639 None:intergenic 45.0%
! TGGTACTGAGCTGAATCATG+GGG + chr8.2:20333038-20333057 MS.gene033324:CDS 45.0%
! TGTGGTACTGAGCTGAATCA+TGG + chr8.2:20333036-20333055 MS.gene033324:CDS 45.0%
!! AAATTTATTAAGTATATATT+TGG - chr8.2:20332689-20332708 None:intergenic 5.0%
CGTGGCATGTCACATGACAA+TGG + chr8.2:20332039-20332058 MS.gene033324:CDS 50.0%
CTGATGCCAAGTCATTCTCC+TGG - chr8.2:20331786-20331805 None:intergenic 50.0%
GGAAGTGAATGGGGAGAACA+GGG + chr8.2:20333126-20333145 MS.gene033324:CDS 50.0%
GGGTATGGAGCAACTCAAGA+TGG + chr8.2:20333072-20333091 MS.gene033324:CDS 50.0%
GTGAATCAAGGATGCAACGG+TGG + chr8.2:20332533-20332552 MS.gene033324:CDS 50.0%
TGCGACACTGCAGTGAATCA+AGG + chr8.2:20332521-20332540 MS.gene033324:CDS 50.0%
TGGCCATCAATTGACACTGC+TGG - chr8.2:20332789-20332808 None:intergenic 50.0%
TGTTGGCAGGGACATTCTCA+TGG - chr8.2:20332809-20332828 None:intergenic 50.0%
! AGGAAGATAGGTGCTGTCAC+CGG + chr8.2:20332111-20332130 MS.gene033324:CDS 50.0%
!! AGGATGCAACGGTGGGTTAA+TGG + chr8.2:20332541-20332560 MS.gene033324:CDS 50.0%
!! TTGGCCTTGATCCTTTACCC+CGG - chr8.2:20332133-20332152 None:intergenic 50.0%
GGGAAGTGAATGGGGAGAAC+AGG + chr8.2:20333125-20333144 MS.gene033324:CDS 55.0%
GGGGTAGCAATTGTGGGGTA+TGG + chr8.2:20333057-20333076 MS.gene033324:CDS 55.0%
GTCACCGGGGTAAAGGATCA+AGG + chr8.2:20332126-20332145 MS.gene033324:CDS 55.0%
! GAAGATAGGTGCTGTCACCG+GGG + chr8.2:20332113-20332132 MS.gene033324:CDS 55.0%
! GGAAGATAGGTGCTGTCACC+GGG + chr8.2:20332112-20332131 MS.gene033324:CDS 55.0%
! AGGTGCTGTCACCGGGGTAA+AGG + chr8.2:20332119-20332138 MS.gene033324:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 20331709 20333278 20331709 ID=MS.gene033324
chr8.2 mRNA 20331709 20333278 20331709 ID=MS.gene033324.t1;Parent=MS.gene033324
chr8.2 exon 20331709 20332156 20331709 ID=MS.gene033324.t1.exon1;Parent=MS.gene033324.t1
chr8.2 CDS 20331709 20332156 20331709 ID=cds.MS.gene033324.t1;Parent=MS.gene033324.t1
chr8.2 exon 20332423 20332655 20332423 ID=MS.gene033324.t1.exon2;Parent=MS.gene033324.t1
chr8.2 CDS 20332423 20332655 20332423 ID=cds.MS.gene033324.t1;Parent=MS.gene033324.t1
chr8.2 exon 20332777 20332917 20332777 ID=MS.gene033324.t1.exon3;Parent=MS.gene033324.t1
chr8.2 CDS 20332777 20332917 20332777 ID=cds.MS.gene033324.t1;Parent=MS.gene033324.t1
chr8.2 exon 20333018 20333278 20333018 ID=MS.gene033324.t1.exon4;Parent=MS.gene033324.t1
chr8.2 CDS 20333018 20333278 20333018 ID=cds.MS.gene033324.t1;Parent=MS.gene033324.t1
Gene Sequence

>MS.gene033324

ATGGAAATGAAGAAGCTCTTGTTTGTTTCCTTATCCCTAGCTTTGATTTTAGGAATAGCTAAGAGCTTTGATTTCCAGGAGAATGACTTGGCATCAGAGAAAAGTTTGTGGGATTTGTACGAGAGATGGAGAAGTCACCACACAGTTACTCGAAGTCTTGATGAGAAGAATAATCGTTTTAATGTGTTCAAAGCCAATGTTATGCATGTCCACAATACCAATAAATTAGATAAGCCTTATAAGCTTAAGTTGAATAAGTTTGCTGACATGACAAATTATGAATTTAGAAGCATCTATGCTGATTCTAAGGTTAATCATCATAGAATGTTCCGTGGCATGTCACATGACAATGGACCGTTCATGTATGAGAATGTGGAGGATGTTCCTTCTTCGATTGATTGGAGGAAGATAGGTGCTGTCACCGGGGTAAAGGATCAAGGCCAATGCGGTAAGCTTACTTGTTGATTAAGTAATCACATACTATTTATAATTTGCGTAAAACATGTTTTTTGATATCTACAAAATACTCACCATGTTAGTTAAATCGCTTGAAGTTTGATAGTTAAGTTTGTTCTTCCACTTAGTCTGTATTTGATTATACGTTTGGATCCAAAATTGATCTGGAGAACTGGAGAAGTGAAATTGATTTTAACATGTTTGATTGTTCTGAAGTAGAATTGATTTGGTCTTTTGAATTGTTTCTAACTTGTATAGGTAGTTGCTGGGCTTTCTCAACAATTGTAGCTGTTGAAGGTATTAACCAAATCAAGACACAAAAACTAGTATCCTTGTCTGAGCAAGAACTTGTGGATTGCGACACTGCAGTGAATCAAGGATGCAACGGTGGGTTAATGGAAAATGCATTCGAGTTCATCAAGCAAAATGGCATAACAACAGAAACTAATTATCCTTATGCTGCAAAAGAGGGAACCTGTAATACACAAAAGGTGCACGCACTAGTCTATTTATAATTAGTACCAAATATATACTTAATAAATTTTATTTTGATTGACTTATGAGAAAATTTAAAGATCCTATTAAGTATTAACACATTTTCACTTGATGTAGGAGAATAAGCCAGCAGTGTCAATTGATGGCCATGAGAATGTCCCTGCCAACAATGAAGCATCATTGTTGAAAGCTGCTGCCAACCAACCTATTTCTGTAGCTATTGATGCTGGTGGATCTGATTTCCAGTTTTATTCAGAGGTAAAACTTATATATTTTTATCCCTTACACATTTATATATTTTTGTATAAATTTGTATATCACCTTGATCCCACTTTTAATTACAAAAATATAATTACAGGGAGTATTTACTGGACATTGTGGTACTGAGCTGAATCATGGGGTAGCAATTGTGGGGTATGGAGCAACTCAAGATGGAACAAAATATTGGACAGTGAAGAACTCTTGGGGAAGTGAATGGGGAGAACAGGGTTACATTAGAATGCAAAGGGACATATCTCACAAGCAAGGTCTATGTGGCATAGCTATGGAGGCTTCCTATCCAATCAAAAAGTCATCTGAAAACCCAACAAAAAGTTCACTTCCAAAAGATGAGCTTTAA
Protein sequence

>MS.gene033324.t1

MEMKKLLFVSLSLALILGIAKSFDFQENDLASEKSLWDLYERWRSHHTVTRSLDEKNNRFNVFKANVMHVHNTNKLDKPYKLKLNKFADMTNYEFRSIYADSKVNHHRMFRGMSHDNGPFMYENVEDVPSSIDWRKIGAVTGVKDQGQCGSCWAFSTIVAVEGINQIKTQKLVSLSEQELVDCDTAVNQGCNGGLMENAFEFIKQNGITTETNYPYAAKEGTCNTQKENKPAVSIDGHENVPANNEASLLKAAANQPISVAIDAGGSDFQFYSEGVFTGHCGTELNHGVAIVGYGATQDGTKYWTVKNSWGSEWGEQGYIRMQRDISHKQGLCGIAMEASYPIKKSSENPTKSSLPKDEL