AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73838

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73838.t1 MTR_4g079440 94.737 304 16 0 1 304 42 345 0.0 606
MS.gene73838.t1 MTR_2g090890 65.017 303 105 1 1 303 35 336 2.58e-149 422
MS.gene73838.t1 MTR_4g107930 57.468 308 125 3 1 302 36 343 4.92e-126 363
MS.gene73838.t1 MTR_5g022560 56.494 308 128 3 1 302 35 342 2.45e-123 357
MS.gene73838.t1 MTR_4g079470 56.209 306 127 4 3 302 39 343 2.60e-122 354
MS.gene73838.t1 MTR_4g079770 56.209 306 127 4 3 302 39 343 3.09e-122 354
MS.gene73838.t1 MTR_4g047610 55.519 308 131 3 1 302 34 341 1.00e-121 352
MS.gene73838.t1 MTR_4g079800 55.556 306 129 4 3 302 39 343 6.94e-120 348
MS.gene73838.t1 MTR_4g080730 53.947 304 137 3 1 302 36 338 2.35e-118 344
MS.gene73838.t1 MTR_4g080700 52.961 304 140 3 1 302 36 338 1.39e-116 339
MS.gene73838.t1 MTR_3g075390 53.398 309 130 5 5 303 40 344 2.75e-114 334
MS.gene73838.t1 MTR_1g107550 54.313 313 130 5 1 303 46 355 7.77e-114 337
MS.gene73838.t1 MTR_4g104370 54.723 307 128 6 5 303 40 343 6.92e-113 331
MS.gene73838.t1 MTR_2g075830 55.082 305 134 3 1 302 35 339 6.36e-111 325
MS.gene73838.t1 MTR_3g116080 52.318 302 138 3 5 302 47 346 1.62e-108 319
MS.gene73838.t1 MTR_4g094918 51.299 308 140 5 5 303 35 341 3.62e-108 318
MS.gene73838.t1 MTR_4g080360 49.677 310 148 5 1 303 33 341 2.36e-107 316
MS.gene73838.t1 MTR_2g083930 48.408 314 152 3 1 304 31 344 3.13e-107 316
MS.gene73838.t1 MTR_3g102220 50.000 314 145 4 1 303 49 361 6.10e-107 319
MS.gene73838.t1 MTR_1g018840 50.000 310 141 6 5 303 55 361 1.10e-102 308
MS.gene73838.t1 MTR_1g018840 50.000 310 141 6 5 303 55 361 1.25e-102 308
MS.gene73838.t1 MTR_3g112430 49.026 308 148 5 3 302 34 340 1.18e-100 302
MS.gene73838.t1 MTR_7g078600 49.511 307 146 7 1 304 38 338 1.08e-95 286
MS.gene73838.t1 MTR_7g079230 47.712 306 150 6 1 302 35 334 8.43e-91 274
MS.gene73838.t1 MTR_2g016180 43.322 307 162 5 5 302 51 354 2.98e-85 264
MS.gene73838.t1 MTR_2g016270 43.750 304 150 6 5 302 51 339 8.54e-84 260
MS.gene73838.t1 MTR_2g015850 45.361 291 148 6 21 304 78 364 1.99e-81 251
MS.gene73838.t1 MTR_2g015920 40.968 310 168 7 5 303 53 358 3.72e-73 229
MS.gene73838.t1 MTR_4g065077 41.534 313 162 11 5 303 51 356 7.34e-71 223
MS.gene73838.t1 MTR_4g125300 41.311 305 168 6 5 304 51 349 3.24e-69 219
MS.gene73838.t1 MTR_2g067900 41.905 315 160 9 4 301 15 323 1.22e-66 211
MS.gene73838.t1 MTR_1g023210 37.658 316 168 10 5 300 51 357 6.54e-66 211
MS.gene73838.t1 MTR_8g086470 39.482 309 158 10 5 303 67 356 1.40e-64 207
MS.gene73838.t1 MTR_2g067560 43.125 320 149 13 4 299 15 325 1.22e-63 216
MS.gene73838.t1 MTR_4g071060 36.741 313 174 8 5 300 48 353 2.47e-63 204
MS.gene73838.t1 MTR_2g064475 39.884 346 148 14 4 301 15 348 1.52e-62 202
MS.gene73838.t1 MTR_2g015930 38.019 313 174 8 5 303 52 358 9.93e-61 197
MS.gene73838.t1 MTR_1g094135 36.086 327 173 11 2 304 53 367 1.59e-59 195
MS.gene73838.t1 MTR_7g078630 40.370 270 124 6 1 259 702 945 6.90e-56 194
MS.gene73838.t1 MTR_2g066980 37.730 326 141 12 3 304 32 319 8.87e-55 181
MS.gene73838.t1 MTR_1g023810 41.743 218 119 5 87 301 49 261 2.32e-53 175
MS.gene73838.t1 MTR_4g072790 33.775 302 164 8 4 300 47 317 1.33e-47 162
MS.gene73838.t1 MTR_4g073660 33.007 306 165 8 4 300 46 320 1.30e-45 157
MS.gene73838.t1 MTR_4g072340 33.007 306 165 8 4 300 47 321 1.56e-45 157
MS.gene73838.t1 MTR_2g015840 32.667 300 136 11 8 304 36 272 3.08e-36 131
MS.gene73838.t1 MTR_7g079130 60.870 92 35 1 213 303 6 97 1.08e-32 116
MS.gene73838.t1 MTR_7g078580 55.556 99 42 2 206 303 4 101 2.37e-31 114
MS.gene73838.t1 MTR_1g025450 36.994 173 86 4 87 256 55 207 2.86e-30 120
MS.gene73838.t1 MTR_2g016380 42.478 113 61 2 193 302 23 134 1.09e-24 100
MS.gene73838.t1 MTR_7g078310 40.411 146 69 4 43 180 112 247 2.24e-24 99.8
MS.gene73838.t1 MTR_8g087510 41.912 136 72 5 128 261 23 153 2.30e-23 94.7
MS.gene73838.t1 MTR_3g029560 27.237 257 152 8 61 304 4 238 1.17e-22 94.4
MS.gene73838.t1 MTR_7g111060 28.205 273 161 10 47 295 69 330 9.02e-20 88.6
MS.gene73838.t1 MTR_7g111050 27.972 286 163 13 50 302 61 336 1.08e-19 88.6
MS.gene73838.t1 MTR_2g047960 51.250 80 38 1 96 175 7 85 1.19e-18 79.7
MS.gene73838.t1 MTR_7g079260 50.000 72 19 2 230 300 7 62 3.89e-15 70.5
MS.gene73838.t1 MTR_3g082370 28.636 220 129 9 92 294 5 213 1.75e-14 72.0
MS.gene73838.t1 MTR_7g111050 26.316 247 145 11 50 265 56 296 2.09e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73838.t1 AT1G06260 59.672 305 120 3 1 303 39 342 3.22e-130 374
MS.gene73838.t1 AT3G48340 56.129 310 121 6 5 303 38 343 2.07e-118 345
MS.gene73838.t1 AT5G43060 53.398 309 137 4 1 303 46 353 4.10e-114 337
MS.gene73838.t1 AT5G50260 54.369 309 127 5 5 303 38 342 1.17e-113 333
MS.gene73838.t1 AT5G45890 51.746 315 139 5 1 304 34 346 3.12e-113 331
MS.gene73838.t1 AT1G47128 52.303 304 139 5 5 303 50 352 3.81e-111 330
MS.gene73838.t1 AT4G35350 52.288 306 141 2 1 302 47 351 2.73e-110 324
MS.gene73838.t1 AT3G48350 52.412 311 133 6 3 302 36 342 3.23e-109 322
MS.gene73838.t1 AT1G20850 50.987 304 141 3 5 302 51 352 1.29e-105 312
MS.gene73838.t1 AT4G11310 50.327 306 143 5 5 303 49 352 5.92e-104 308
MS.gene73838.t1 AT4G11320 50.654 306 142 6 5 303 56 359 3.01e-103 306
MS.gene73838.t1 AT4G11320 50.654 306 142 6 5 303 56 359 3.01e-103 306
MS.gene73838.t1 AT4G36880 50.482 311 138 6 8 303 52 361 3.18e-102 304
MS.gene73838.t1 AT3G19390 48.039 306 153 4 2 302 40 344 5.91e-102 306
MS.gene73838.t1 AT4G23520 48.852 305 148 7 5 303 47 349 1.95e-101 301
MS.gene73838.t1 AT2G27420 49.371 318 141 7 3 303 33 347 3.37e-101 300
MS.gene73838.t1 AT3G19400 47.403 308 156 4 1 302 40 347 9.18e-101 300
MS.gene73838.t1 AT3G49340 49.521 313 141 8 3 303 33 340 1.26e-100 299
MS.gene73838.t1 AT1G09850 47.883 307 155 3 1 303 28 333 3.11e-99 298
MS.gene73838.t1 AT2G34080 46.326 313 155 6 1 303 35 344 2.04e-93 281
MS.gene73838.t1 AT1G29080 46.278 309 148 8 8 303 42 345 7.44e-88 266
MS.gene73838.t1 AT1G29090 45.338 311 149 8 8 303 50 354 2.00e-85 261
MS.gene73838.t1 AT4G35350 50.000 242 115 3 1 238 47 286 6.96e-79 241
MS.gene73838.t1 AT3G43960 43.182 308 164 8 5 303 41 346 7.57e-79 244
MS.gene73838.t1 AT1G29110 41.694 307 154 6 8 303 41 333 2.14e-75 234
MS.gene73838.t1 AT3G48350 47.431 253 119 5 3 245 36 284 2.84e-75 233
MS.gene73838.t1 AT5G60360 41.639 305 167 5 5 304 59 357 8.46e-73 228
MS.gene73838.t1 AT5G60360 41.311 305 167 6 5 304 59 356 2.45e-70 222
MS.gene73838.t1 AT3G19400 44.939 247 130 4 1 241 40 286 1.76e-69 218
MS.gene73838.t1 AT3G45310 39.344 305 174 6 5 304 59 357 2.59e-69 219
MS.gene73838.t1 AT5G60360 40.678 295 164 5 5 294 59 347 1.71e-67 215
MS.gene73838.t1 AT3G45310 39.016 305 174 7 5 304 59 356 8.49e-67 213
MS.gene73838.t1 AT4G39090 38.006 321 160 10 5 300 51 357 3.92e-66 211
MS.gene73838.t1 AT2G21430 36.246 309 172 8 5 295 48 349 3.27e-61 199
MS.gene73838.t1 AT3G54940 36.677 319 175 8 2 300 48 359 3.24e-58 191
MS.gene73838.t1 AT4G16190 36.190 315 176 8 5 300 55 363 1.12e-56 187
MS.gene73838.t1 AT3G54940 35.652 230 136 3 2 220 48 276 7.93e-41 143
MS.gene73838.t1 AT1G02305 28.671 286 156 12 43 295 63 333 5.48e-21 92.0
MS.gene73838.t1 AT4G01610 27.174 276 162 10 50 295 64 330 9.05e-21 91.7
MS.gene73838.t1 AT4G01610 26.812 276 163 10 50 295 64 330 1.51e-19 88.2
MS.gene73838.t1 AT1G02300 28.910 211 123 7 106 295 146 350 6.08e-15 75.1

Find 72 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTCCAATCCTTGCTTTC+TGG 0.072633 4.2:+30835771 None:intergenic
AGAGTTTCAAAGTACTTATA+TGG 0.243800 4.2:-30835856 MS.gene73838:CDS
ACCTTCAAACCTCTTCTTCA+TGG 0.288966 4.2:+30836034 None:intergenic
CTTATATGGGTCTTGGTACT+AGG 0.296317 4.2:-30835842 MS.gene73838:CDS
GACGAACGGGAAGTCCGTTT+TGG 0.317728 4.2:-30835975 MS.gene73838:CDS
GCTGCAGTGGCAGCTGTAGA+AGG 0.321629 4.2:-30835451 MS.gene73838:CDS
TTTATTGTCTGTGAGATTGT+AGG 0.348908 4.2:+30835899 None:intergenic
AGGTTGCCAAGGTGGCTTAA+TGG 0.360502 4.2:-30835347 MS.gene73838:CDS
GGTGCTGACTGGGGTGAATC+TGG 0.360846 4.2:-30834983 MS.gene73838:CDS
GGGATCAACAAAATAAAATC+AGG 0.374637 4.2:-30835430 MS.gene73838:CDS
AGAATTCATGGGGTGCTGAC+TGG 0.387691 4.2:-30834994 MS.gene73838:CDS
ATTGGATTGTGAAGAATTCA+TGG 0.392004 4.2:-30835006 MS.gene73838:CDS
CAAAGTACTTATATGGGTCT+TGG 0.395625 4.2:-30835849 MS.gene73838:CDS
GGATTCAGGTATGATGAAAA+TGG 0.398407 4.2:-30835801 MS.gene73838:CDS
TGTTCACACAGGAGGTTGTT+GGG 0.402344 4.2:-30835479 MS.gene73838:intron
AAGGAAATATTAATAAGTAT+TGG 0.416014 4.2:-30835024 MS.gene73838:CDS
GTTCACATAATACAGGATTC+AGG 0.420869 4.2:-30835815 MS.gene73838:CDS
GAATTCATGGGGTGCTGACT+GGG 0.423926 4.2:-30834993 MS.gene73838:CDS
GAAGAAGAGGTTTGAAGGTT+GGG 0.426580 4.2:-30836030 MS.gene73838:CDS
GGTGTAACCGTAGTTGGATA+TGG 0.429893 4.2:-30835049 MS.gene73838:CDS
GAGTTTCAAAGTACTTATAT+GGG 0.441325 4.2:-30835855 MS.gene73838:CDS
CATTATGCTGCGAGTATAAG+TGG 0.447078 4.2:-30835223 MS.gene73838:CDS
TATCCTTATGAAGGTGTAGA+TGG 0.448214 4.2:-30835271 MS.gene73838:CDS
AGCTTAGCCTCATTATCAGC+AGG 0.460600 4.2:+30835187 None:intergenic
AATCATGGTGTAACCGTAGT+TGG 0.476071 4.2:-30835055 MS.gene73838:CDS
ATACAAACATAATGACGAAC+GGG 0.476584 4.2:-30835988 MS.gene73838:CDS
TTATATGGGTCTTGGTACTA+GGG 0.478572 4.2:-30835841 MS.gene73838:CDS
TTAAGCCACCTTGGCAACCT+TGG 0.486289 4.2:+30835350 None:intergenic
GCCATGAAGAAGAGGTTTGA+AGG 0.488543 4.2:-30836035 MS.gene73838:CDS
TTGCTTGGTAAATGCCAAAA+CGG 0.493062 4.2:+30835961 None:intergenic
TGAAGAAGAGGTTTGAAGGT+TGG 0.494459 4.2:-30836031 MS.gene73838:CDS
GGGGTGAATCTGGTTATATC+AGG 0.495176 4.2:-30834973 MS.gene73838:CDS
GTGTTCACACAGGAGGTTGT+TGG 0.498927 4.2:-30835480 MS.gene73838:intron
TCCTTCTCCATATCCAACTA+CGG 0.504152 4.2:+30835042 None:intergenic
CGCGACACTTTGAGTAAAGA+AGG 0.504741 4.2:-30834944 MS.gene73838:CDS
AATAAGCTAAACTACACTAG+AGG 0.504746 4.2:+30834890 None:intergenic
ATCTTCCAGAAAGCAAGGAT+TGG 0.515492 4.2:-30835776 MS.gene73838:CDS
TCATTTCAGCTTTATTCAGA+AGG 0.521702 4.2:-30835109 MS.gene73838:CDS
AAACTACACTAGAGGGTAGC+TGG 0.525707 4.2:+30834898 None:intergenic
GTACCATCTACACCTTCATA+AGG 0.526767 4.2:+30835268 None:intergenic
TTCCAGAAAGCAAGGATTGG+AGG 0.532858 4.2:-30835773 MS.gene73838:CDS
GTAACTGAAATCAAGAATCA+AGG 0.536484 4.2:-30835738 MS.gene73838:CDS
TGTGGAAAGCAACTCAATCA+TGG 0.536875 4.2:-30835070 MS.gene73838:CDS
TGCATCAATTGCAACAGACA+CGG 0.539151 4.2:+30835141 None:intergenic
GTATGTATTGAACATTTGCT+TGG 0.546306 4.2:+30835946 None:intergenic
AGCAAGGATTGGAGGAAGGA+GGG 0.547902 4.2:-30835765 MS.gene73838:CDS
AGGGTGCGTTCACATAATAC+AGG 0.550141 4.2:-30835822 MS.gene73838:CDS
CTGCAGTGGCAGCTGTAGAA+GGG 0.550833 4.2:-30835450 MS.gene73838:CDS
TTGTTGGGCATTCGCTGCAG+TGG 0.553618 4.2:-30835464 MS.gene73838:CDS
TGGTGATCTTCCAGAAAGCA+AGG 0.571198 4.2:-30835781 MS.gene73838:CDS
ACCGTAGTTGGATATGGAGA+AGG 0.573204 4.2:-30835043 MS.gene73838:CDS
AGAAGTTCCTGCTGATAATG+AGG 0.576412 4.2:-30835194 MS.gene73838:CDS
AATGGTAACCAAGGTTGCCA+AGG 0.576933 4.2:-30835358 MS.gene73838:CDS
AAGCAAGGATTGGAGGAAGG+AGG 0.583928 4.2:-30835766 MS.gene73838:CDS
GTGTCTGTTGCAATTGATGC+AGG 0.584191 4.2:-30835139 MS.gene73838:CDS
AATACAAACATAATGACGAA+CGG 0.584501 4.2:-30835989 MS.gene73838:CDS
CTGTCTCCATTAAGCCACCT+TGG 0.587016 4.2:+30835341 None:intergenic
ACGTAGAAGCCATGAAGAAG+AGG 0.591122 4.2:-30836043 None:intergenic
GAAGGTTGGGTGAAACGACA+TGG 0.591844 4.2:-30836017 MS.gene73838:CDS
GCATCAATTGCAACAGACAC+GGG 0.598881 4.2:+30835142 None:intergenic
GGTAACCAAGGTTGCCAAGG+TGG 0.606188 4.2:-30835355 MS.gene73838:CDS
ATAAGCTAAACTACACTAGA+GGG 0.610529 4.2:+30834891 None:intergenic
TTGGATTGTGAAGAATTCAT+GGG 0.612300 4.2:-30835005 MS.gene73838:CDS
AGAAAGCAAGGATTGGAGGA+AGG 0.613399 4.2:-30835769 MS.gene73838:CDS
GGATCAACAAAATAAAATCA+GGG 0.617083 4.2:-30835429 MS.gene73838:CDS
AATTCATGGGGTGCTGACTG+GGG 0.644464 4.2:-30834992 MS.gene73838:CDS
TGTAGATGGTACCTGTAAGA+CGG 0.648789 4.2:-30835257 MS.gene73838:CDS
TTGAGTAAAGAAGGTATGTG+TGG 0.649760 4.2:-30834935 MS.gene73838:CDS
TACAAGAAACATTACCACAC+TGG 0.663674 4.2:+30835715 None:intergenic
ATCAAGAATCAAGGCCAGTG+TGG 0.700928 4.2:-30835729 MS.gene73838:intron
TATACTCGCAGCATAATGTG+CGG 0.723834 4.2:+30835228 None:intergenic
TGGATTGTGAAGAATTCATG+GGG 0.729679 4.2:-30835004 MS.gene73838:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGGAAATATTAATAAGTAT+TGG - chr4.2:30835909-30835928 MS.gene73838:CDS 15.0%
!! ATATCAAGTTAAAAAAACAT+TGG + chr4.2:30835363-30835382 None:intergenic 15.0%
!! TATACATTCATCATAAAAAA+TGG - chr4.2:30835617-30835636 MS.gene73838:intron 15.0%
!! ATAGATTGTGATGTTAAAAA+TGG - chr4.2:30835557-30835576 MS.gene73838:intron 20.0%
! AATACAAACATAATGACGAA+CGG - chr4.2:30834944-30834963 MS.gene73838:CDS 25.0%
! ACATTCATCATAAAAAATGG+CGG - chr4.2:30835620-30835639 MS.gene73838:intron 25.0%
! ATGTGATTTCTTACAACTTA+AGG - chr4.2:30835322-30835341 MS.gene73838:CDS 25.0%
! GGATCAACAAAATAAAATCA+GGG - chr4.2:30835504-30835523 MS.gene73838:intron 25.0%
!! AGAGTTTCAAAGTACTTATA+TGG - chr4.2:30835077-30835096 MS.gene73838:CDS 25.0%
!! GAGTTTCAAAGTACTTATAT+GGG - chr4.2:30835078-30835097 MS.gene73838:CDS 25.0%
!! TTCATAAGGATAGTCTTTTT+CGG + chr4.2:30835654-30835673 None:intergenic 25.0%
!!! AACATTGGTATTTTAACTTG+TGG - chr4.2:30835390-30835409 MS.gene73838:CDS 25.0%
ATACAAACATAATGACGAAC+GGG - chr4.2:30834945-30834964 MS.gene73838:CDS 30.0%
ATAGCTTGAATCGCATAATA+TGG + chr4.2:30835295-30835314 None:intergenic 30.0%
ATTGGATTGTGAAGAATTCA+TGG - chr4.2:30835927-30835946 MS.gene73838:CDS 30.0%
CTTGATATTGTACGAAACAT+TGG - chr4.2:30835375-30835394 MS.gene73838:CDS 30.0%
GAAAAAGACTATCCTTATGA+AGG - chr4.2:30835653-30835672 MS.gene73838:intron 30.0%
GATGTTAAAAATGGTAACCA+AGG - chr4.2:30835566-30835585 MS.gene73838:intron 30.0%
GGGATCAACAAAATAAAATC+AGG - chr4.2:30835503-30835522 MS.gene73838:intron 30.0%
GTAACTGAAATCAAGAATCA+AGG - chr4.2:30835195-30835214 MS.gene73838:CDS 30.0%
GTATGTATTGAACATTTGCT+TGG + chr4.2:30834990-30835009 None:intergenic 30.0%
TTGGATTGTGAAGAATTCAT+GGG - chr4.2:30835928-30835947 MS.gene73838:CDS 30.0%
TTTATTGTCTGTGAGATTGT+AGG + chr4.2:30835037-30835056 None:intergenic 30.0%
TTTCTTGTAACAGAATTGCA+TGG - chr4.2:30835232-30835251 MS.gene73838:CDS 30.0%
!! TCATTTCAGCTTTATTCAGA+AGG - chr4.2:30835824-30835843 MS.gene73838:CDS 30.0%
!!! TTTTTAGAGGTTCTAGGTTA+TGG - chr4.2:30835256-30835275 MS.gene73838:CDS 30.0%
GGATTCAGGTATGATGAAAA+TGG - chr4.2:30835132-30835151 MS.gene73838:CDS 35.0%
GTTCACATAATACAGGATTC+AGG - chr4.2:30835118-30835137 MS.gene73838:CDS 35.0%
TACAAGAAACATTACCACAC+TGG + chr4.2:30835221-30835240 None:intergenic 35.0%
TGGATTGTGAAGAATTCATG+GGG - chr4.2:30835929-30835948 MS.gene73838:CDS 35.0%
TTATATGGGTCTTGGTACTA+GGG - chr4.2:30835092-30835111 MS.gene73838:CDS 35.0%
TTGCTTGGTAAATGCCAAAA+CGG + chr4.2:30834975-30834994 None:intergenic 35.0%
! AGAATTGCATGGCTTTTTAG+AGG - chr4.2:30835243-30835262 MS.gene73838:CDS 35.0%
! CAAAGTACTTATATGGGTCT+TGG - chr4.2:30835084-30835103 MS.gene73838:CDS 35.0%
! TATCCTTATGAAGGTGTAGA+TGG - chr4.2:30835662-30835681 MS.gene73838:intron 35.0%
! TTGAGTAAAGAAGGTATGTG+TGG - chr4.2:30835998-30836017 MS.gene73838:CDS 35.0%
!!! ATAAGGATAGTCTTTTTCGG+TGG + chr4.2:30835651-30835670 None:intergenic 35.0%
!!! CTTTTAAGTTGTGTTCACAC+AGG - chr4.2:30835443-30835462 MS.gene73838:CDS 35.0%
!!! TATTCAGAAGGTGTTTTCTC+TGG - chr4.2:30835836-30835855 MS.gene73838:CDS 35.0%
AATCATGGTGTAACCGTAGT+TGG - chr4.2:30835878-30835897 MS.gene73838:CDS 40.0%
ATCTTCCAGAAAGCAAGGAT+TGG - chr4.2:30835157-30835176 MS.gene73838:CDS 40.0%
CATTATGCTGCGAGTATAAG+TGG - chr4.2:30835710-30835729 MS.gene73838:intron 40.0%
CTTATATGGGTCTTGGTACT+AGG - chr4.2:30835091-30835110 MS.gene73838:CDS 40.0%
GAAGAAGAGGTTTGAAGGTT+GGG - chr4.2:30834903-30834922 MS.gene73838:CDS 40.0%
GTACCATCTACACCTTCATA+AGG + chr4.2:30835668-30835687 None:intergenic 40.0%
TATACTCGCAGCATAATGTG+CGG + chr4.2:30835708-30835727 None:intergenic 40.0%
TCCTTCTCCATATCCAACTA+CGG + chr4.2:30835894-30835913 None:intergenic 40.0%
TGAAGAAGAGGTTTGAAGGT+TGG - chr4.2:30834902-30834921 MS.gene73838:CDS 40.0%
TGCATCAATTGCAACAGACA+CGG + chr4.2:30835795-30835814 None:intergenic 40.0%
TGTAGATGGTACCTGTAAGA+CGG - chr4.2:30835676-30835695 MS.gene73838:intron 40.0%
TGTGGAAAGCAACTCAATCA+TGG - chr4.2:30835863-30835882 MS.gene73838:CDS 40.0%
TGTGGAACATTCTCTCAAGA+TGG - chr4.2:30835408-30835427 MS.gene73838:CDS 40.0%
! AGAAGTTCCTGCTGATAATG+AGG - chr4.2:30835739-30835758 MS.gene73838:CDS 40.0%
! TTAAGTTGTGTTCACACAGG+AGG - chr4.2:30835446-30835465 MS.gene73838:CDS 40.0%
!! CATGGCTTTTTAGAGGTTCT+AGG - chr4.2:30835250-30835269 MS.gene73838:CDS 40.0%
AATGGTAACCAAGGTTGCCA+AGG - chr4.2:30835575-30835594 MS.gene73838:intron 45.0%
ACCGTAGTTGGATATGGAGA+AGG - chr4.2:30835890-30835909 MS.gene73838:CDS 45.0%
AGAAAGCAAGGATTGGAGGA+AGG - chr4.2:30835164-30835183 MS.gene73838:CDS 45.0%
AGCTTAGCCTCATTATCAGC+AGG + chr4.2:30835749-30835768 None:intergenic 45.0%
AGGGTGCGTTCACATAATAC+AGG - chr4.2:30835111-30835130 MS.gene73838:CDS 45.0%
ATCAAGAATCAAGGCCAGTG+TGG - chr4.2:30835204-30835223 MS.gene73838:CDS 45.0%
CGCGACACTTTGAGTAAAGA+AGG - chr4.2:30835989-30836008 MS.gene73838:CDS 45.0%
GCATCAATTGCAACAGACAC+GGG + chr4.2:30835794-30835813 None:intergenic 45.0%
GGGGTGAATCTGGTTATATC+AGG - chr4.2:30835960-30835979 MS.gene73838:CDS 45.0%
GGTGTAACCGTAGTTGGATA+TGG - chr4.2:30835884-30835903 MS.gene73838:CDS 45.0%
GTGTCTGTTGCAATTGATGC+AGG - chr4.2:30835794-30835813 MS.gene73838:CDS 45.0%
TGGTGATCTTCCAGAAAGCA+AGG - chr4.2:30835152-30835171 MS.gene73838:CDS 45.0%
TGTTCACACAGGAGGTTGTT+GGG - chr4.2:30835454-30835473 MS.gene73838:CDS 45.0%
TTCCAGAAAGCAAGGATTGG+AGG - chr4.2:30835160-30835179 MS.gene73838:CDS 45.0%
TTCCTCCAATCCTTGCTTTC+TGG + chr4.2:30835165-30835184 None:intergenic 45.0%
! GGTGTTTTCTCTGGCATTTG+TGG - chr4.2:30835845-30835864 MS.gene73838:CDS 45.0%
AAGCAAGGATTGGAGGAAGG+AGG - chr4.2:30835167-30835186 MS.gene73838:CDS 50.0%
AGCAAGGATTGGAGGAAGGA+GGG - chr4.2:30835168-30835187 MS.gene73838:CDS 50.0%
AGGTTGCCAAGGTGGCTTAA+TGG - chr4.2:30835586-30835605 MS.gene73838:intron 50.0%
CTGTCTCCATTAAGCCACCT+TGG + chr4.2:30835595-30835614 None:intergenic 50.0%
GAAGGTTGGGTGAAACGACA+TGG - chr4.2:30834916-30834935 MS.gene73838:CDS 50.0%
GTGTTCACACAGGAGGTTGT+TGG - chr4.2:30835453-30835472 MS.gene73838:CDS 50.0%
TTAAGCCACCTTGGCAACCT+TGG + chr4.2:30835586-30835605 None:intergenic 50.0%
!! AATTCATGGGGTGCTGACTG+GGG - chr4.2:30835941-30835960 MS.gene73838:CDS 50.0%
!! AGAATTCATGGGGTGCTGAC+TGG - chr4.2:30835939-30835958 MS.gene73838:CDS 50.0%
!! GAATTCATGGGGTGCTGACT+GGG - chr4.2:30835940-30835959 MS.gene73838:CDS 50.0%
!! TGCGGCTTTTTCCGTCTTAC+AGG + chr4.2:30835690-30835709 None:intergenic 50.0%
CTGCAGTGGCAGCTGTAGAA+GGG - chr4.2:30835483-30835502 MS.gene73838:intron 55.0%
GGTAACCAAGGTTGCCAAGG+TGG - chr4.2:30835578-30835597 MS.gene73838:intron 55.0%
TTGTTGGGCATTCGCTGCAG+TGG - chr4.2:30835469-30835488 MS.gene73838:CDS 55.0%
! GACGAACGGGAAGTCCGTTT+TGG - chr4.2:30834958-30834977 MS.gene73838:CDS 55.0%
GCTGCAGTGGCAGCTGTAGA+AGG - chr4.2:30835482-30835501 MS.gene73838:intron 60.0%
!! GGTGCTGACTGGGGTGAATC+TGG - chr4.2:30835950-30835969 MS.gene73838:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 30834901 30836054 30834901 ID=MS.gene73838
chr4.2 mRNA 30834901 30836054 30834901 ID=MS.gene73838.t1;Parent=MS.gene73838
chr4.2 exon 30835730 30836054 30835730 ID=MS.gene73838.t1.exon1;Parent=MS.gene73838.t1
chr4.2 CDS 30835730 30836054 30835730 ID=cds.MS.gene73838.t1;Parent=MS.gene73838.t1
chr4.2 exon 30834901 30835490 30834901 ID=MS.gene73838.t1.exon2;Parent=MS.gene73838.t1
chr4.2 CDS 30834901 30835490 30834901 ID=cds.MS.gene73838.t1;Parent=MS.gene73838.t1
Gene Sequence

>MS.gene73838

ATGAAGAAGAGGTTTGAAGGTTGGGTGAAACGACATGGTCGAAAATACAAACATAATGACGAACGGGAAGTCCGTTTTGGCATTTACCAAGCAAATGTTCAATACATACATTGCAAAAACGCACAGAAAAACTCCTACAATCTCACAGACAATAAATTTGCAGATCTTACGAATGAAGAGTTTCAAAGTACTTATATGGGTCTTGGTACTAGGGTGCGTTCACATAATACAGGATTCAGGTATGATGAAAATGGTGATCTTCCAGAAAGCAAGGATTGGAGGAAGGAGGGAGCAGTAACTGAAATCAAGAATCAAGGCCAGTGTGGTAATGTTTCTTGTAACAGAATTGCATGGCTTTTTAGAGGTTCTAGGTTATGGTATTTCAGTTTTACCATATTATGCGATTCAAGCTATGTTACTTATGTGATTTCTTACAACTTAAGGAACATTCTTACTCTGCCAATGTTTTTTTAACTTGATATTGTACGAAACATTGGTATTTTAACTTGTGGAACATTCTCTCAAGATGGTTGTTTTAACTACTTTTAAGTTGTGTTCACACAGGAGGTTGTTGGGCATTCGCTGCAGTGGCAGCTGTAGAAGGGATCAACAAAATAAAATCAGGGAAATTGATCTCTCTATCTGAACAAGAACTAATAGATTGTGATGTTAAAAATGGTAACCAAGGTTGCCAAGGTGGCTTAATGGAGACAGCATATACATTCATCATAAAAAATGGCGGACTAACCACCGAAAAAGACTATCCTTATGAAGGTGTAGATGGTACCTGTAAGACGGAAAAAGCCGCACATTATGCTGCGAGTATAAGTGGATATGAAGAAGTTCCTGCTGATAATGAGGCTAAGCTTAAAGCTGCTGCAGCTCATCAACCCGTGTCTGTTGCAATTGATGCAGGAAGTTATTCATTTCAGCTTTATTCAGAAGGTGTTTTCTCTGGCATTTGTGGAAAGCAACTCAATCATGGTGTAACCGTAGTTGGATATGGAGAAGGAAATATTAATAAGTATTGGATTGTGAAGAATTCATGGGGTGCTGACTGGGGTGAATCTGGTTATATCAGGATGAAGCGCGACACTTTGAGTAAAGAAGGTATGTGTGGCATTGCTATGCAAGCCAGCTACCCTCTAGTGTAG
Protein sequence

>MS.gene73838.t1

MKKRFEGWVKRHGRKYKHNDEREVRFGIYQANVQYIHCKNAQKNSYNLTDNKFADLTNEEFQSTYMGLGTRVRSHNTGFRYDENGDLPESKDWRKEGAVTEIKNQGQCGGCWAFAAVAAVEGINKIKSGKLISLSEQELIDCDVKNGNQGCQGGLMETAYTFIIKNGGLTTEKDYPYEGVDGTCKTEKAAHYAASISGYEEVPADNEAKLKAAAAHQPVSVAIDAGSYSFQLYSEGVFSGICGKQLNHGVTVVGYGEGNINKYWIVKNSWGADWGESGYIRMKRDTLSKEGMCGIAMQASYPLV