AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022410.t1 MTR_3g075390 97.238 362 10 0 1 362 1 362 0.0 742
MS.gene022410.t1 MTR_4g104370 76.519 362 83 2 1 362 1 360 0.0 594
MS.gene022410.t1 MTR_4g080700 57.692 312 121 7 35 343 35 338 3.85e-125 363
MS.gene022410.t1 MTR_4g080730 57.051 312 123 7 35 343 35 338 1.32e-124 362
MS.gene022410.t1 MTR_5g022560 55.031 318 129 6 33 343 32 342 2.78e-123 359
MS.gene022410.t1 MTR_4g107930 54.574 317 128 5 35 343 35 343 6.37e-123 358
MS.gene022410.t1 MTR_4g079770 51.724 348 158 7 1 343 1 343 7.26e-123 358
MS.gene022410.t1 MTR_4g047610 57.325 314 123 7 38 344 33 342 8.17e-123 358
MS.gene022410.t1 MTR_4g079470 54.517 321 137 6 28 344 29 344 8.27e-123 358
MS.gene022410.t1 MTR_1g107550 55.215 326 141 5 23 345 33 356 3.06e-122 361
MS.gene022410.t1 MTR_3g116080 51.289 349 159 5 3 345 5 348 4.00e-122 356
MS.gene022410.t1 MTR_4g094918 51.320 341 151 5 8 345 14 342 1.28e-121 355
MS.gene022410.t1 MTR_1g018840 51.130 354 160 7 5 346 11 363 1.41e-121 358
MS.gene022410.t1 MTR_1g018840 51.576 349 160 6 6 346 16 363 2.71e-121 358
MS.gene022410.t1 MTR_4g079800 52.615 325 137 6 28 344 29 344 6.55e-121 353
MS.gene022410.t1 MTR_3g102220 51.989 352 156 8 6 345 12 362 2.20e-120 356
MS.gene022410.t1 MTR_2g075830 53.488 344 145 7 5 343 6 339 3.59e-118 346
MS.gene022410.t1 MTR_4g079440 50.000 344 156 6 6 344 12 344 4.87e-117 343
MS.gene022410.t1 MTR_2g090890 50.000 348 162 5 1 346 1 338 1.26e-115 339
MS.gene022410.t1 MTR_4g080360 51.466 307 145 3 40 344 37 341 1.82e-112 331
MS.gene022410.t1 MTR_2g083930 46.608 339 170 5 10 344 12 343 5.63e-106 315
MS.gene022410.t1 MTR_7g078600 46.483 327 158 7 25 347 27 340 1.43e-99 298
MS.gene022410.t1 MTR_7g079230 45.376 346 174 7 1 343 1 334 1.69e-98 296
MS.gene022410.t1 MTR_3g112430 48.875 311 156 3 39 347 35 344 3.87e-98 298
MS.gene022410.t1 MTR_2g016180 43.939 330 167 11 25 345 36 356 1.63e-83 262
MS.gene022410.t1 MTR_2g016270 43.465 329 155 10 25 345 36 341 2.93e-83 261
MS.gene022410.t1 MTR_2g015850 45.638 298 156 5 48 344 71 363 3.76e-82 254
MS.gene022410.t1 MTR_4g065077 43.380 355 175 13 6 345 14 357 2.68e-76 239
MS.gene022410.t1 MTR_2g015920 40.116 344 186 12 27 361 41 373 1.19e-73 233
MS.gene022410.t1 MTR_4g125300 43.960 298 147 9 53 344 65 348 7.00e-73 230
MS.gene022410.t1 MTR_8g086470 42.571 350 169 12 6 345 30 357 1.46e-69 222
MS.gene022410.t1 MTR_2g015930 37.978 366 186 16 6 345 9 359 9.09e-66 212
MS.gene022410.t1 MTR_2g067900 41.860 301 158 11 55 344 32 326 1.67e-62 203
MS.gene022410.t1 MTR_1g023210 39.799 299 152 9 54 336 66 352 4.41e-62 203
MS.gene022410.t1 MTR_4g071060 38.127 299 156 8 54 336 63 348 2.51e-59 196
MS.gene022410.t1 MTR_2g064475 38.484 343 161 13 40 342 16 348 7.36e-59 194
MS.gene022410.t1 MTR_2g067560 40.432 324 152 15 40 338 16 323 1.40e-58 204
MS.gene022410.t1 MTR_1g023810 42.731 227 122 6 119 342 40 261 9.54e-57 186
MS.gene022410.t1 MTR_7g078630 42.652 279 112 10 40 304 706 950 3.41e-54 192
MS.gene022410.t1 MTR_2g066980 35.505 307 141 11 55 343 50 317 3.44e-53 179
MS.gene022410.t1 MTR_1g094135 31.965 341 197 10 15 336 34 358 2.73e-52 178
MS.gene022410.t1 MTR_2g015840 31.429 315 144 8 32 343 25 270 7.15e-44 153
MS.gene022410.t1 MTR_4g072790 34.965 286 147 7 54 336 63 312 1.32e-43 154
MS.gene022410.t1 MTR_7g078580 63.636 99 35 1 246 344 4 101 1.82e-42 144
MS.gene022410.t1 MTR_4g072340 34.021 291 147 9 54 336 63 316 5.52e-41 147
MS.gene022410.t1 MTR_4g073660 34.364 291 146 10 54 336 62 315 5.56e-41 147
MS.gene022410.t1 MTR_7g079130 63.736 91 33 0 254 344 7 97 1.88e-37 130
MS.gene022410.t1 MTR_2g047960 75.949 79 19 0 137 215 7 85 1.84e-35 125
MS.gene022410.t1 MTR_1g025450 41.954 174 77 7 127 296 54 207 6.56e-33 129
MS.gene022410.t1 MTR_2g016380 45.902 122 59 4 228 345 18 136 2.20e-28 111
MS.gene022410.t1 MTR_7g111060 27.174 276 165 11 86 336 66 330 9.02e-24 100
MS.gene022410.t1 MTR_7g111050 30.328 244 138 10 130 350 106 340 1.15e-22 97.8
MS.gene022410.t1 MTR_3g029560 32.547 212 114 8 139 344 49 237 5.84e-21 90.9
MS.gene022410.t1 MTR_7g079260 55.556 72 16 1 270 341 7 62 6.46e-20 84.3
MS.gene022410.t1 MTR_7g078310 35.429 175 94 6 48 217 84 244 3.06e-19 86.7
MS.gene022410.t1 MTR_8g087510 40.769 130 71 5 169 296 23 148 1.71e-18 82.4
MS.gene022410.t1 MTR_7g111050 29.146 199 118 7 130 307 101 297 6.15e-15 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022410.t1 AT5G50260 70.278 360 106 1 4 362 2 361 0.0 556
MS.gene022410.t1 AT3G48340 67.313 361 115 2 4 362 2 361 0.0 518
MS.gene022410.t1 AT3G48350 64.167 360 127 2 4 362 2 360 4.11e-177 496
MS.gene022410.t1 AT3G48350 65.580 276 93 2 5 278 2 277 2.78e-134 385
MS.gene022410.t1 AT1G20850 54.348 322 143 3 25 345 36 354 2.21e-122 357
MS.gene022410.t1 AT4G35350 51.576 349 159 4 3 345 9 353 2.60e-122 357
MS.gene022410.t1 AT1G47128 49.280 347 165 4 6 346 13 354 3.87e-118 350
MS.gene022410.t1 AT5G43060 50.571 350 157 5 6 345 11 354 5.35e-118 350
MS.gene022410.t1 AT3G19390 48.159 353 174 6 1 350 5 351 1.52e-115 343
MS.gene022410.t1 AT5G45890 47.143 350 167 7 6 343 1 344 1.02e-114 337
MS.gene022410.t1 AT4G36880 50.760 329 151 6 32 351 41 367 3.72e-113 334
MS.gene022410.t1 AT3G19400 51.592 314 142 7 39 347 43 351 1.05e-107 320
MS.gene022410.t1 AT1G06260 48.000 350 166 7 1 345 5 343 3.07e-106 315
MS.gene022410.t1 AT4G11310 46.518 359 171 8 3 347 4 355 1.82e-105 314
MS.gene022410.t1 AT4G11320 46.218 357 170 8 6 347 13 362 3.26e-103 309
MS.gene022410.t1 AT4G11320 46.218 357 170 8 6 347 13 362 3.26e-103 309
MS.gene022410.t1 AT2G27420 45.821 347 173 5 6 344 8 347 1.11e-99 299
MS.gene022410.t1 AT1G09850 49.355 310 150 4 38 345 30 334 1.52e-99 301
MS.gene022410.t1 AT4G23520 42.458 358 190 7 3 350 4 355 9.98e-99 297
MS.gene022410.t1 AT3G49340 44.412 340 182 5 9 344 4 340 3.43e-98 295
MS.gene022410.t1 AT2G34080 44.638 345 180 6 6 344 5 344 9.48e-95 286
MS.gene022410.t1 AT1G29090 45.000 360 167 11 1 344 10 354 9.38e-92 279
MS.gene022410.t1 AT1G29080 41.595 351 192 8 1 344 1 345 1.70e-88 270
MS.gene022410.t1 AT3G43960 45.846 325 160 11 39 354 40 357 1.74e-88 271
MS.gene022410.t1 AT4G35350 49.291 282 133 4 3 278 9 286 7.04e-88 266
MS.gene022410.t1 AT1G29110 39.316 351 188 9 1 344 1 333 4.20e-79 246
MS.gene022410.t1 AT3G19400 50.417 240 110 6 39 273 43 278 1.15e-72 228
MS.gene022410.t1 AT5G60360 41.667 300 155 9 51 344 71 356 2.70e-69 221
MS.gene022410.t1 AT3G45310 41.060 302 154 10 51 344 71 356 5.33e-67 216
MS.gene022410.t1 AT5G60360 41.333 300 155 10 51 344 71 355 9.65e-67 215
MS.gene022410.t1 AT3G45310 40.728 302 154 11 51 344 71 355 2.12e-64 209
MS.gene022410.t1 AT5G60360 41.429 280 148 8 51 324 71 340 3.56e-64 208
MS.gene022410.t1 AT2G21430 33.903 351 198 10 6 336 13 349 8.41e-61 199
MS.gene022410.t1 AT4G39090 37.124 299 160 8 54 336 66 352 1.59e-57 191
MS.gene022410.t1 AT3G54940 37.793 299 160 9 54 336 66 354 2.97e-55 185
MS.gene022410.t1 AT4G16190 37.584 298 160 9 55 336 71 358 8.71e-54 182
MS.gene022410.t1 AT3G54940 37.674 215 122 4 54 260 66 276 4.46e-39 140
MS.gene022410.t1 AT1G02305 27.893 337 208 11 50 362 33 358 1.02e-27 112
MS.gene022410.t1 AT4G01610 29.352 293 166 13 85 350 65 343 1.21e-23 100
MS.gene022410.t1 AT4G01610 28.669 293 168 13 85 350 65 343 9.30e-22 95.5
MS.gene022410.t1 AT1G02300 31.799 239 131 10 147 362 146 375 3.61e-21 94.0
MS.gene022410.t1 AT2G22160 46.465 99 49 1 53 151 8 102 7.21e-21 87.0

Find 91 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGCCTATTTGTCTTTATT+TGG 0.139485 3.2:+68054565 None:intergenic
CCGTTGATGGAACTAATTAT+TGG 0.225728 3.2:-68053916 MS.gene022410:CDS
ATCAGATCCTCCGGCATCTA+TGG 0.300046 3.2:+68054182 None:intergenic
ACATGACCAACCATGAATTT+AGG 0.309976 3.2:-68055114 MS.gene022410:CDS
AAGCTCCTGCTTCTGTTGAT+TGG 0.324173 3.2:-68055000 MS.gene022410:CDS
GAACTCATGGGGAGCGGAAT+GGG 0.328427 3.2:-68053885 MS.gene022410:CDS
TTTGAGTACATCAAGCAAAA+AGG 0.333516 3.2:-68054462 MS.gene022410:CDS
CCGAGGACACATCTTTATCT+TGG 0.334739 3.2:+68055305 None:intergenic
TAAACCAAATAAAGACAAAT+AGG 0.344680 3.2:-68054569 MS.gene022410:CDS
TGTCCTGTATAGGTAGTTGC+TGG 0.345293 3.2:-68054623 MS.gene022410:intron
GCATCTATGGCTACGGATAC+AGG 0.385812 3.2:+68054195 None:intergenic
TCAACTGTTGTAGCTGTTGA+AGG 0.388321 3.2:-68054594 MS.gene022410:CDS
TTGTTAAGTTTCAACTTGTA+AGG 0.389898 3.2:+68055145 None:intergenic
AGGATGCAATGGTGGGCTAA+TGG 0.392108 3.2:-68054493 MS.gene022410:CDS
GATCCATCATTTGCTGTGTA+AGG 0.397020 3.2:+68054414 None:intergenic
CACTTCCACGTGGCGTGCCT+CGG 0.411657 3.2:+68055050 None:intergenic
GATTGTTCTGTCCATTGCTT+TGG 0.412192 3.2:-68055356 MS.gene022410:CDS
ATCAACAGAAGCAGGAGCTT+TGG 0.424537 3.2:+68055003 None:intergenic
TGACCATCAATTGACACAGC+TGG 0.433204 3.2:+68054273 None:intergenic
GGTGTAGCAATAGTAGGATA+TGG 0.443941 3.2:-68053944 MS.gene022410:CDS
TGATGGTCATGAGACTGTTC+CGG 0.449890 3.2:-68054259 MS.gene022410:CDS
GGAGTCACCACACGGTTTCT+CGG 0.450148 3.2:-68055249 MS.gene022410:CDS
GGATATGGAACAACCGTTGA+TGG 0.454887 3.2:-68053929 MS.gene022410:CDS
GTCCCAGCTGTGTCAATTGA+TGG 0.458384 3.2:-68054276 MS.gene022410:CDS
CGAGGCACGCCACGTGGAAG+TGG 0.460548 3.2:-68055049 MS.gene022410:CDS
TCATCCTTGGTAGATGATAA+AGG 0.462977 3.2:+68053749 None:intergenic
TTTCTCCAATCAACAGAAGC+AGG 0.466214 3.2:+68054995 None:intergenic
TATCCATCTTGTTAGTGTTG+TGG 0.468486 3.2:+68055170 None:intergenic
CATAGGTAGTCCTAAATTCA+TGG 0.476124 3.2:+68055104 None:intergenic
ATTGGATTGTGAGGAACTCA+TGG 0.493389 3.2:-68053898 MS.gene022410:CDS
CTATTACAGGGAGTATTTAG+TGG 0.493552 3.2:-68053992 MS.gene022410:intron
AATCATGGTGTAGCAATAGT+AGG 0.495226 3.2:-68053950 MS.gene022410:CDS
GTCACTGAGGTCAAGGATCA+AGG 0.495703 3.2:-68054962 MS.gene022410:CDS
TCTAACAAGGAAGGACTTTG+TGG 0.498260 3.2:-68053827 MS.gene022410:CDS
GTAGATGATAAAGGTCCTGC+TGG 0.507201 3.2:+68053758 None:intergenic
AAACATTCAAAGCTCATCCT+TGG 0.508057 3.2:+68053736 None:intergenic
TACCCTTACACAGCAAATGA+TGG 0.512248 3.2:-68054417 MS.gene022410:CDS
GAATTTAGGACTACCTATGC+TGG 0.513702 3.2:-68055100 MS.gene022410:CDS
GGAACTCATGGGGAGCGGAA+TGG 0.524402 3.2:-68053886 MS.gene022410:CDS
GTCCTCGGACAAAAGCTTGT+GGG 0.525849 3.2:-68055290 MS.gene022410:CDS
AGGACTTTGTGGCATAGCTA+TGG 0.526821 3.2:-68053816 MS.gene022410:CDS
TGTGACAATCAAGAAAATCA+AGG 0.527505 3.2:-68054513 MS.gene022410:CDS
GAAAATCAAGGATGCAATGG+TGG 0.535112 3.2:-68054501 MS.gene022410:CDS
GTCCTGTATAGGTAGTTGCT+GGG 0.537057 3.2:-68054622 MS.gene022410:intron
TCCTCCGGCATCTATGGCTA+CGG 0.543132 3.2:+68054188 None:intergenic
GACTACCTATGCTGGATCAA+AGG 0.546319 3.2:-68055092 MS.gene022410:CDS
CAAGAAAATCAAGGATGCAA+TGG 0.548537 3.2:-68054504 MS.gene022410:CDS
ACTTCTTTAACTTACCACAT+TGG 0.551760 3.2:+68054939 None:intergenic
AAACTGGAAATCAGATCCTC+CGG 0.552551 3.2:+68054173 None:intergenic
TCTGTTGATTGGAGAAAGAA+AGG 0.553167 3.2:-68054989 MS.gene022410:CDS
GGCTACGGATACAGGTTGGT+TGG 0.553342 3.2:+68054203 None:intergenic
AGGTGCTGTCACTGAGGTCA+AGG 0.558185 3.2:-68054969 MS.gene022410:CDS
ATCCATCATTTGCTGTGTAA+GGG 0.560080 3.2:+68054415 None:intergenic
TGTCCACAACACTAACAAGA+TGG 0.563716 3.2:-68055173 MS.gene022410:CDS
TGTGGTAAAGAGCTAAATCA+TGG 0.564153 3.2:-68053965 MS.gene022410:CDS
CCAAGATAAAGATGTGTCCT+CGG 0.565632 3.2:-68055305 MS.gene022410:CDS
GACCATCAATTGACACAGCT+GGG 0.568817 3.2:+68054274 None:intergenic
CCAATAATTAGTTCCATCAA+CGG 0.573198 3.2:+68053916 None:intergenic
TGTGGGACTTATACGAGAGA+TGG 0.573304 3.2:-68055273 MS.gene022410:CDS
TTGGATTGTGAGGAACTCAT+GGG 0.574375 3.2:-68053897 MS.gene022410:CDS
AACTCATGGGGAGCGGAATG+GGG 0.577276 3.2:-68053884 MS.gene022410:CDS
AAAGCATCTTCATCGTTAGC+CGG 0.584931 3.2:+68054240 None:intergenic
AAATTCATGGTTGGTCATGT+CGG 0.587621 3.2:+68055117 None:intergenic
GGAGCGGAATGGGGTGAACA+AGG 0.588520 3.2:-68053875 MS.gene022410:CDS
ATTAACCTTTGATCCAGCAT+AGG 0.590030 3.2:+68055087 None:intergenic
ATCTTGGAAATCGAAGCTCT+CGG 0.596119 3.2:+68055321 None:intergenic
GGAGTATTTAGTGGCGATTG+TGG 0.600793 3.2:-68053983 MS.gene022410:CDS
TACTTCAAGAACCAAAGCAA+TGG 0.600914 3.2:+68055345 None:intergenic
TGTGAGGAACTCATGGGGAG+CGG 0.601438 3.2:-68053891 MS.gene022410:CDS
TGTCCTCGGACAAAAGCTTG+TGG 0.602219 3.2:-68055291 MS.gene022410:CDS
ATGTTCCGAGGCACGCCACG+TGG 0.610728 3.2:-68055055 MS.gene022410:CDS
GCGTGCCTCGGAACATTCTG+TGG 0.611727 3.2:+68055062 None:intergenic
AGGAATGTATCTAACAAGGA+AGG 0.612463 3.2:-68053836 MS.gene022410:CDS
GAACTAATTATTGGATTGTG+AGG 0.613342 3.2:-68053907 MS.gene022410:CDS
GGTAGTCCTAAATTCATGGT+TGG 0.613532 3.2:+68055108 None:intergenic
AAAATCAAGGATGCAATGGT+GGG 0.617377 3.2:-68054500 MS.gene022410:CDS
ATAAGCTCTTGTTCAGACAA+AGG 0.634536 3.2:+68054540 None:intergenic
AAGGTTACATCAGAATGCAA+AGG 0.641547 3.2:-68053856 MS.gene022410:CDS
GCAAAGGAATGTATCTAACA+AGG 0.642601 3.2:-68053840 MS.gene022410:CDS
CTATGGCTACGGATACAGGT+TGG 0.643007 3.2:+68054199 None:intergenic
GGGACTTATACGAGAGATGG+AGG 0.652822 3.2:-68055270 MS.gene022410:CDS
GTATCCGTAGCCATAGATGC+CGG 0.655334 3.2:-68054192 MS.gene022410:CDS
AGGACCTTTATCATCTACCA+AGG 0.670444 3.2:-68053753 MS.gene022410:CDS
AATCACCACAGAATGTTCCG+AGG 0.693791 3.2:-68055067 MS.gene022410:CDS
TCCGTAGCCATAGATGCCGG+AGG 0.697215 3.2:-68054189 MS.gene022410:CDS
GTCAAGGATCAAGGCCAATG+TGG 0.699707 3.2:-68054953 MS.gene022410:intron
GAGATGGAGGAGTCACCACA+CGG 0.702988 3.2:-68055257 MS.gene022410:CDS
TGGATTGTGAGGAACTCATG+GGG 0.709984 3.2:-68053896 MS.gene022410:CDS
CAAGGTTCCGAGAAACCGTG+TGG 0.715039 3.2:+68055242 None:intergenic
AAAGAAAGGTGCTGTCACTG+AGG 0.722701 3.2:-68054975 MS.gene022410:CDS
ATGAATGTTCCACTTCCACG+TGG 0.756623 3.2:+68055040 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTACTTTTTTATTTTGA+AGG + chr3.2:68055037-68055056 None:intergenic 10.0%
!! GAAAAAAAAATATATGATCT+AGG + chr3.2:68054428-68054447 None:intergenic 15.0%
!! TAAAAAAGTAAAAAAAGAGA+TGG - chr3.2:68055043-68055062 MS.gene022410:CDS 15.0%
!!! ATGAATAATTTTTGAATCAT+AGG + chr3.2:68054812-68054831 None:intergenic 15.0%
!! TAAACCAAATAAAGACAAAT+AGG - chr3.2:68054552-68054571 MS.gene022410:CDS 20.0%
! TATAATTGACATGCAACTTT+AGG - chr3.2:68054784-68054803 MS.gene022410:intron 25.0%
! TCAAAAATTATTCATGTTGC+AGG - chr3.2:68054817-68054836 MS.gene022410:intron 25.0%
! TTGTTAAGTTTCAACTTGTA+AGG + chr3.2:68053979-68053998 None:intergenic 25.0%
!! ATTTTGTATACACCTATTAC+AGG - chr3.2:68055116-68055135 MS.gene022410:CDS 25.0%
!! TTTTGTATACACCTATTACA+GGG - chr3.2:68055117-68055136 MS.gene022410:CDS 25.0%
CCAATAATTAGTTCCATCAA+CGG + chr3.2:68055208-68055227 None:intergenic 30.0%
CTAGCCTATTTGTCTTTATT+TGG + chr3.2:68054559-68054578 None:intergenic 30.0%
GAACTAATTATTGGATTGTG+AGG - chr3.2:68055214-68055233 MS.gene022410:CDS 30.0%
TGTGACAATCAAGAAAATCA+AGG - chr3.2:68054608-68054627 MS.gene022410:CDS 30.0%
TTGTGATTAATGTCCTGTAT+AGG - chr3.2:68054488-68054507 MS.gene022410:CDS 30.0%
TTTGAGTACATCAAGCAAAA+AGG - chr3.2:68054659-68054678 MS.gene022410:intron 30.0%
! ACTTCTTTAACTTACCACAT+TGG + chr3.2:68054185-68054204 None:intergenic 30.0%
! TTTTTACCTCTGAGTAAAAC+TGG + chr3.2:68054967-68054986 None:intergenic 30.0%
!! TTTTGGAGGAGTTTTTAACT+GGG + chr3.2:68055341-68055360 None:intergenic 30.0%
!! TTTTTGGAGGAGTTTTTAAC+TGG + chr3.2:68055342-68055361 None:intergenic 30.0%
AAAATCAAGGATGCAATGGT+GGG - chr3.2:68054621-68054640 MS.gene022410:intron 35.0%
AAGGTTACATCAGAATGCAA+AGG - chr3.2:68055265-68055284 MS.gene022410:CDS 35.0%
AATCATGGTGTAGCAATAGT+AGG - chr3.2:68055171-68055190 MS.gene022410:CDS 35.0%
ACATGACCAACCATGAATTT+AGG - chr3.2:68054007-68054026 MS.gene022410:intron 35.0%
AGGAATGTATCTAACAAGGA+AGG - chr3.2:68055285-68055304 MS.gene022410:CDS 35.0%
ATAAGCTCTTGTTCAGACAA+AGG + chr3.2:68054584-68054603 None:intergenic 35.0%
ATTAACCTTTGATCCAGCAT+AGG + chr3.2:68054037-68054056 None:intergenic 35.0%
ATTCTCTTGTGTCAATAGAG+AGG + chr3.2:68055001-68055020 None:intergenic 35.0%
CAAGAAAATCAAGGATGCAA+TGG - chr3.2:68054617-68054636 MS.gene022410:intron 35.0%
CACTAAATACTCCCTGTAAT+AGG + chr3.2:68055131-68055150 None:intergenic 35.0%
CATAGGTAGTCCTAAATTCA+TGG + chr3.2:68054020-68054039 None:intergenic 35.0%
GACACCATCTAATCAAAGAT+CGG - chr3.2:68054255-68054274 MS.gene022410:CDS 35.0%
GCAAAGGAATGTATCTAACA+AGG - chr3.2:68055281-68055300 MS.gene022410:CDS 35.0%
TACTTCAAGAACCAAAGCAA+TGG + chr3.2:68053779-68053798 None:intergenic 35.0%
TATCCATCTTGTTAGTGTTG+TGG + chr3.2:68053954-68053973 None:intergenic 35.0%
TCATCCTTGGTAGATGATAA+AGG + chr3.2:68055375-68055394 None:intergenic 35.0%
TGTGGTAAAGAGCTAAATCA+TGG - chr3.2:68055156-68055175 MS.gene022410:CDS 35.0%
! AAATTCATGGTTGGTCATGT+CGG + chr3.2:68054007-68054026 None:intergenic 35.0%
! CCGTTGATGGAACTAATTAT+TGG - chr3.2:68055205-68055224 MS.gene022410:CDS 35.0%
! TCTGTTGATTGGAGAAAGAA+AGG - chr3.2:68054132-68054151 MS.gene022410:intron 35.0%
! TGATTTCCAGTTTTACTCAG+AGG - chr3.2:68054958-68054977 MS.gene022410:CDS 35.0%
!! ATCCATCATTTGCTGTGTAA+GGG + chr3.2:68054709-68054728 None:intergenic 35.0%
!! CTATTACAGGGAGTATTTAG+TGG - chr3.2:68055129-68055148 MS.gene022410:CDS 35.0%
AAACTGGAAATCAGATCCTC+CGG + chr3.2:68054951-68054970 None:intergenic 40.0%
ACTGTGTGAATGCAAAGTGA+TGG + chr3.2:68054226-68054245 None:intergenic 40.0%
AGGACCTTTATCATCTACCA+AGG - chr3.2:68055368-68055387 MS.gene022410:CDS 40.0%
ATCTTGGAAATCGAAGCTCT+CGG + chr3.2:68053803-68053822 None:intergenic 40.0%
ATTGGATTGTGAGGAACTCA+TGG - chr3.2:68055223-68055242 MS.gene022410:CDS 40.0%
CCAAGATAAAGATGTGTCCT+CGG - chr3.2:68053816-68053835 MS.gene022410:CDS 40.0%
GAAAATCAAGGATGCAATGG+TGG - chr3.2:68054620-68054639 MS.gene022410:intron 40.0%
GAGTACATCAAGCAAAAAGG+TGG - chr3.2:68054662-68054681 MS.gene022410:intron 40.0%
GATTGTTCTGTCCATTGCTT+TGG - chr3.2:68053765-68053784 MS.gene022410:CDS 40.0%
GGTAGTCCTAAATTCATGGT+TGG + chr3.2:68054016-68054035 None:intergenic 40.0%
GGTGTAGCAATAGTAGGATA+TGG - chr3.2:68055177-68055196 MS.gene022410:CDS 40.0%
TACCCTTACACAGCAAATGA+TGG - chr3.2:68054704-68054723 MS.gene022410:intron 40.0%
TCAACTGTTGTAGCTGTTGA+AGG - chr3.2:68054527-68054546 MS.gene022410:CDS 40.0%
TCGTCCGATCTTTGATTAGA+TGG + chr3.2:68054262-68054281 None:intergenic 40.0%
TCTAACAAGGAAGGACTTTG+TGG - chr3.2:68055294-68055313 MS.gene022410:CDS 40.0%
TGTCCACAACACTAACAAGA+TGG - chr3.2:68053948-68053967 MS.gene022410:CDS 40.0%
TTGGATTGTGAGGAACTCAT+GGG - chr3.2:68055224-68055243 MS.gene022410:CDS 40.0%
TTTCTCCAATCAACAGAAGC+AGG + chr3.2:68054129-68054148 None:intergenic 40.0%
! AAAGCATCTTCATCGTTAGC+CGG + chr3.2:68054884-68054903 None:intergenic 40.0%
! GAATTTAGGACTACCTATGC+TGG - chr3.2:68054021-68054040 MS.gene022410:intron 40.0%
! TAAAGGTCCTGCTGGATTTT+TGG + chr3.2:68055358-68055377 None:intergenic 40.0%
!! GATCCATCATTTGCTGTGTA+AGG + chr3.2:68054710-68054729 None:intergenic 40.0%
AACTCCTCCAAAAATCCAGC+AGG - chr3.2:68055348-68055367 MS.gene022410:CDS 45.0%
AAGCTCCTGCTTCTGTTGAT+TGG - chr3.2:68054121-68054140 MS.gene022410:intron 45.0%
AATCACCACAGAATGTTCCG+AGG - chr3.2:68054054-68054073 MS.gene022410:intron 45.0%
AGGACTTTGTGGCATAGCTA+TGG - chr3.2:68055305-68055324 MS.gene022410:CDS 45.0%
ATCAACAGAAGCAGGAGCTT+TGG + chr3.2:68054121-68054140 None:intergenic 45.0%
ATGAATGTTCCACTTCCACG+TGG + chr3.2:68054084-68054103 None:intergenic 45.0%
CCGAGGACACATCTTTATCT+TGG + chr3.2:68053819-68053838 None:intergenic 45.0%
GACCATCAATTGACACAGCT+GGG + chr3.2:68054850-68054869 None:intergenic 45.0%
GACTACCTATGCTGGATCAA+AGG - chr3.2:68054029-68054048 MS.gene022410:intron 45.0%
GGATATGGAACAACCGTTGA+TGG - chr3.2:68055192-68055211 MS.gene022410:CDS 45.0%
GTAGATGATAAAGGTCCTGC+TGG + chr3.2:68055366-68055385 None:intergenic 45.0%
GTCCTGTATAGGTAGTTGCT+GGG - chr3.2:68054499-68054518 MS.gene022410:CDS 45.0%
TGACCATCAATTGACACAGC+TGG + chr3.2:68054851-68054870 None:intergenic 45.0%
TGATGGTCATGAGACTGTTC+CGG - chr3.2:68054862-68054881 MS.gene022410:intron 45.0%
TGGATTGTGAGGAACTCATG+GGG - chr3.2:68055225-68055244 MS.gene022410:CDS 45.0%
TGTCCTGTATAGGTAGTTGC+TGG - chr3.2:68054498-68054517 MS.gene022410:CDS 45.0%
TGTGGGACTTATACGAGAGA+TGG - chr3.2:68053848-68053867 MS.gene022410:CDS 45.0%
! GGAGTATTTAGTGGCGATTG+TGG - chr3.2:68055138-68055157 MS.gene022410:CDS 45.0%
!! AAAGAAAGGTGCTGTCACTG+AGG - chr3.2:68054146-68054165 MS.gene022410:intron 45.0%
!! AGCGCTTTTGTTTCTCGTCA+AGG + chr3.2:68053900-68053919 None:intergenic 45.0%
!! GGAGGAGTTTTTAACTGGGT+AGG + chr3.2:68055337-68055356 None:intergenic 45.0%
AGCCCAGCAACTACCTATAC+AGG + chr3.2:68054504-68054523 None:intergenic 50.0%
ATCAGATCCTCCGGCATCTA+TGG + chr3.2:68054942-68054961 None:intergenic 50.0%
CTATGGCTACGGATACAGGT+TGG + chr3.2:68054925-68054944 None:intergenic 50.0%
GCATCTATGGCTACGGATAC+AGG + chr3.2:68054929-68054948 None:intergenic 50.0%
GGGACTTATACGAGAGATGG+AGG - chr3.2:68053851-68053870 MS.gene022410:CDS 50.0%
GTATCCGTAGCCATAGATGC+CGG - chr3.2:68054929-68054948 MS.gene022410:intron 50.0%
GTCAAGGATCAAGGCCAATG+TGG - chr3.2:68054168-68054187 MS.gene022410:CDS 50.0%
GTCACTGAGGTCAAGGATCA+AGG - chr3.2:68054159-68054178 MS.gene022410:intron 50.0%
GTCCCAGCTGTGTCAATTGA+TGG - chr3.2:68054845-68054864 MS.gene022410:intron 50.0%
GTCCTCGGACAAAAGCTTGT+GGG - chr3.2:68053831-68053850 MS.gene022410:CDS 50.0%
TGTCCTCGGACAAAAGCTTG+TGG - chr3.2:68053830-68053849 MS.gene022410:CDS 50.0%
!! AGGATGCAATGGTGGGCTAA+TGG - chr3.2:68054628-68054647 MS.gene022410:intron 50.0%
!! AGGTCCTGCTGGATTTTTGG+AGG + chr3.2:68055355-68055374 None:intergenic 50.0%
AACTCATGGGGAGCGGAATG+GGG - chr3.2:68055237-68055256 MS.gene022410:CDS 55.0%
CAAGGTTCCGAGAAACCGTG+TGG + chr3.2:68053882-68053901 None:intergenic 55.0%
GAACTCATGGGGAGCGGAAT+GGG - chr3.2:68055236-68055255 MS.gene022410:CDS 55.0%
GAGATGGAGGAGTCACCACA+CGG - chr3.2:68053864-68053883 MS.gene022410:CDS 55.0%
GGAGTCACCACACGGTTTCT+CGG - chr3.2:68053872-68053891 MS.gene022410:CDS 55.0%
TCCTCCGGCATCTATGGCTA+CGG + chr3.2:68054936-68054955 None:intergenic 55.0%
TGTGAGGAACTCATGGGGAG+CGG - chr3.2:68055230-68055249 MS.gene022410:CDS 55.0%
! AGGTGCTGTCACTGAGGTCA+AGG - chr3.2:68054152-68054171 MS.gene022410:intron 55.0%
! GTCCCACAAGCTTTTGTCCG+AGG + chr3.2:68053836-68053855 None:intergenic 55.0%
!! GGCTACGGATACAGGTTGGT+TGG + chr3.2:68054921-68054940 None:intergenic 55.0%
GCGTGCCTCGGAACATTCTG+TGG + chr3.2:68054062-68054081 None:intergenic 60.0%
GGAACTCATGGGGAGCGGAA+TGG - chr3.2:68055235-68055254 MS.gene022410:CDS 60.0%
GGAGCGGAATGGGGTGAACA+AGG - chr3.2:68055246-68055265 MS.gene022410:CDS 60.0%
TCCGTAGCCATAGATGCCGG+AGG - chr3.2:68054932-68054951 MS.gene022410:intron 60.0%
ATGTTCCGAGGCACGCCACG+TGG - chr3.2:68054066-68054085 MS.gene022410:intron 65.0%
CACTTCCACGTGGCGTGCCT+CGG + chr3.2:68054074-68054093 None:intergenic 65.0%
CGAGGCACGCCACGTGGAAG+TGG - chr3.2:68054072-68054091 MS.gene022410:intron 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 68053742 68055401 68053742 ID=MS.gene022410
chr3.2 mRNA 68053742 68055401 68053742 ID=MS.gene022410.t1;Parent=MS.gene022410
chr3.2 exon 68054954 68055401 68054954 ID=MS.gene022410.t1.exon1;Parent=MS.gene022410.t1
chr3.2 CDS 68054954 68055401 68054954 ID=cds.MS.gene022410.t1;Parent=MS.gene022410.t1
chr3.2 exon 68054398 68054633 68054398 ID=MS.gene022410.t1.exon2;Parent=MS.gene022410.t1
chr3.2 CDS 68054398 68054633 68054398 ID=cds.MS.gene022410.t1;Parent=MS.gene022410.t1
chr3.2 exon 68054164 68054304 68054164 ID=MS.gene022410.t1.exon3;Parent=MS.gene022410.t1
chr3.2 CDS 68054164 68054304 68054164 ID=cds.MS.gene022410.t1;Parent=MS.gene022410.t1
chr3.2 exon 68053742 68054005 68053742 ID=MS.gene022410.t1.exon4;Parent=MS.gene022410.t1
chr3.2 CDS 68053742 68054005 68053742 ID=cds.MS.gene022410.t1;Parent=MS.gene022410.t1
Gene Sequence

>MS.gene022410

ATGGAAACTAAGAAACTATTGTTGATTGTTCTGTCCATTGCTTTGGTTCTTGAAGTATCCGAGAGCTTCGATTTCCAAGATAAAGATGTGTCCTCGGACAAAAGCTTGTGGGACTTATACGAGAGATGGAGGAGTCACCACACGGTTTCTCGGAACCTTGACGAGAAACAAAAGCGCTTTAACGTGTTCAAATCAAATGTGATGCATGTCCACAACACTAACAAGATGGATAAGCCTTACAAGTTGAAACTTAACAAGTTTGCCGACATGACCAACCATGAATTTAGGACTACCTATGCTGGATCAAAGGTTAATCACCACAGAATGTTCCGAGGCACGCCACGTGGAAGTGGAACATTCATGTATGAAAATTTTACCAAAGCTCCTGCTTCTGTTGATTGGAGAAAGAAAGGTGCTGTCACTGAGGTCAAGGATCAAGGCCAATGTGGTAAGTTAAAGAAGTTATTATTGAGAATCTTCACCATCACTTTGCATTCACACAGTCAACAGTTTGACACCATCTAATCAAAGATCGGACGACTCTTTGTTACGAGCAAATTTTTTAAATATTTAGAAATTCAATTCACTTACTGACTACATAAATGCATATGATTTCTGACTGCAAGTCAATCTATTTTCAAATAGTTATACTAGTCATTATTAATCATATTCATAGAGCTTAACCTAGATCATATATTTTTTTTTCTCACACTATTATGCAATATAGCTTTTTATAACATTGTTGTTTGTGATTAATGTCCTGTATAGGTAGTTGCTGGGCTTTTTCAACTGTTGTAGCTGTTGAAGGCATAAACCAAATAAAGACAAATAGGCTAGTGCCTTTGTCTGAACAAGAGCTTATAGATTGTGACAATCAAGAAAATCAAGGATGCAATGGTGGGCTAATGGAATATGCTTTTGAGTACATCAAGCAAAAAGGTGGCATAACAACAGAAAGCTATTACCCTTACACAGCAAATGATGGATCATGTGATGCAACAAAAGTGAGTAAAGATGCTAAACTTCTATTGAAATTTTTTAATATAATTGACATGCAACTTTAGGAACCTATGATTCAAAAATTATTCATGTTGCAGGAAAATGTCCCAGCTGTGTCAATTGATGGTCATGAGACTGTTCCGGCTAACGATGAAGATGCTTTGCTCAAAGCTGTTGCCAACCAACCTGTATCCGTAGCCATAGATGCCGGAGGATCTGATTTCCAGTTTTACTCAGAGGTAAAAATGAGAATGAATCCTCTCTATTGACACAAGAGAATAATGCAATCTAAACCTTCAAAATAAAAAAGTAAAAAAAGAGATGGAAGAAATATGCTATTATTAGCAATCAATTTTGAAACTAATGTGTATCTTAATTTTGTATACACCTATTACAGGGAGTATTTAGTGGCGATTGTGGTAAAGAGCTAAATCATGGTGTAGCAATAGTAGGATATGGAACAACCGTTGATGGAACTAATTATTGGATTGTGAGGAACTCATGGGGAGCGGAATGGGGTGAACAAGGTTACATCAGAATGCAAAGGAATGTATCTAACAAGGAAGGACTTTGTGGCATAGCTATGGAAGCTTCCTACCCAGTTAAAAACTCCTCCAAAAATCCAGCAGGACCTTTATCATCTACCAAGGATGAGCTTTGA
Protein sequence

>MS.gene022410.t1

METKKLLLIVLSIALVLEVSESFDFQDKDVSSDKSLWDLYERWRSHHTVSRNLDEKQKRFNVFKSNVMHVHNTNKMDKPYKLKLNKFADMTNHEFRTTYAGSKVNHHRMFRGTPRGSGTFMYENFTKAPASVDWRKKGAVTEVKDQGQCGSCWAFSTVVAVEGINQIKTNRLVPLSEQELIDCDNQENQGCNGGLMEYAFEYIKQKGGITTESYYPYTANDGSCDATKENVPAVSIDGHETVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFSGDCGKELNHGVAIVGYGTTVDGTNYWIVRNSWGAEWGEQGYIRMQRNVSNKEGLCGIAMEASYPVKNSSKNPAGPLSSTKDEL