AlfalfaGEDB | MS.gene78715

M. sativa cultivar XinJiangDaYe / MS.gene78715

KEGG: K16292
GO: GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00112.26 PF08246.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78715.t1	XP_013460973.1	96.7	362	12	0	1	362	1	362	3.80E-203	717.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78715.t1	P12412	82.6	362	63	0	1	362	1	362	6.7e-177	621.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78715.t1	G8A2F7	96.7	362	12	0	1	362	1	362	2.7e-203	717.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049227	MS.gene78715	0.813051	3.11E-51	-1.69E-46
MS.gene049807	MS.gene78715	0.833006	6.83E-56	-1.69E-46
MS.gene050414	MS.gene78715	0.806596	7.62E-50	-1.69E-46
MS.gene051234	MS.gene78715	0.808518	2.98E-50	-1.69E-46
MS.gene052016	MS.gene78715	0.800612	1.33E-48	-1.69E-46
MS.gene052307	MS.gene78715	0.800766	1.24E-48	-1.69E-46
MS.gene052628	MS.gene78715	0.87887	1.98E-69	-1.69E-46
MS.gene052630	MS.gene78715	0.924185	9.87E-90	-1.69E-46
MS.gene053143	MS.gene78715	0.842382	2.68E-58	-1.69E-46
MS.gene054178	MS.gene78715	0.82211	2.82E-53	-1.69E-46
MS.gene05894	MS.gene78715	0.820994	5.11E-53	-1.69E-46
MS.gene059778	MS.gene78715	0.823412	1.40E-53	-1.69E-46
MS.gene061100	MS.gene78715	0.832184	1.09E-55	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene031581	MS.gene78715	PPI
MS.gene043473	MS.gene78715	PPI
MS.gene026873	MS.gene78715	PPI
MS.gene006646	MS.gene78715	PPI
MS.gene012206	MS.gene78715	PPI
MS.gene26669	MS.gene78715	PPI
MS.gene78715	MS.gene045520	PPI
MS.gene73817	MS.gene78715	PPI
MS.gene95543	MS.gene78715	PPI
MS.gene028850	MS.gene78715	PPI
MS.gene069978	MS.gene78715	PPI
MS.gene50738	MS.gene78715	PPI
MS.gene31410	MS.gene78715	PPI
MS.gene006665	MS.gene78715	PPI
MS.gene51064	MS.gene78715	PPI
MS.gene78715	MS.gene045522	PPI
MS.gene026876	MS.gene78715	PPI
MS.gene014304	MS.gene78715	PPI
MS.gene24818	MS.gene78715	PPI
MS.gene23321	MS.gene78715	PPI
MS.gene69195	MS.gene78715	PPI
MS.gene78715	MS.gene006680	PPI
MS.gene63396	MS.gene78715	PPI
MS.gene003680	MS.gene78715	PPI
MS.gene058156	MS.gene78715	PPI
MS.gene67468	MS.gene78715	PPI
MS.gene031638	MS.gene78715	PPI
MS.gene73838	MS.gene78715	PPI
MS.gene045458	MS.gene78715	PPI
MS.gene064873	MS.gene78715	PPI
MS.gene63719	MS.gene78715	PPI
MS.gene031619	MS.gene78715	PPI
MS.gene031580	MS.gene78715	PPI
MS.gene78715	MS.gene006720	PPI
MS.gene73835	MS.gene78715	PPI
MS.gene045525	MS.gene78715	PPI
MS.gene87639	MS.gene78715	PPI
MS.gene031635	MS.gene78715	PPI
MS.gene26853	MS.gene78715	PPI
MS.gene027420	MS.gene78715	PPI
MS.gene73834	MS.gene78715	PPI
MS.gene73815	MS.gene78715	PPI
MS.gene005933	MS.gene78715	PPI
MS.gene006682	MS.gene78715	PPI
MS.gene78715	MS.gene26670	PPI
MS.gene78715	MS.gene005934	PPI
MS.gene045455	MS.gene78715	PPI
MS.gene78715	MS.gene065787	PPI
MS.gene045459	MS.gene78715	PPI
MS.gene045526	MS.gene78715	PPI
MS.gene66833	MS.gene78715	PPI
MS.gene033151	MS.gene78715	PPI
MS.gene78715	MS.gene64795	PPI
MS.gene09529	MS.gene78715	PPI
MS.gene25255	MS.gene78715	PPI
MS.gene055760	MS.gene78715	PPI
MS.gene045461	MS.gene78715	PPI
MS.gene031621	MS.gene78715	PPI
MS.gene78715	MS.gene045503	PPI
MS.gene065785	MS.gene78715	PPI
MS.gene26670	MS.gene78715	PPI
MS.gene003677	MS.gene78715	PPI
MS.gene060218	MS.gene78715	PPI
MS.gene73840	MS.gene78715	PPI
MS.gene026874	MS.gene78715	PPI
MS.gene59493	MS.gene78715	PPI
MS.gene34090	MS.gene78715	PPI
MS.gene045501	MS.gene78715	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78715.t1	MTR_3g075390	96.685	362	12	0	1	362	1	362	0.0	739
MS.gene78715.t1	MTR_4g104370	76.519	362	83	2	1	362	1	360	0.0	594
MS.gene78715.t1	MTR_4g080700	58.013	312	120	7	35	343	35	338	1.89e-125	364
MS.gene78715.t1	MTR_4g080730	57.372	312	122	7	35	343	35	338	7.24e-125	363
MS.gene78715.t1	MTR_4g107930	55.205	317	126	5	35	343	35	343	6.11e-124	360
MS.gene78715.t1	MTR_5g022560	55.172	319	129	6	33	344	32	343	1.58e-123	359
MS.gene78715.t1	MTR_4g079470	54.829	321	136	6	28	344	29	344	3.20e-123	358
MS.gene78715.t1	MTR_4g079770	52.011	348	157	7	1	343	1	343	3.24e-123	358
MS.gene78715.t1	MTR_4g047610	57.643	314	122	7	38	344	33	342	3.34e-123	358
MS.gene78715.t1	MTR_1g107550	55.521	326	140	5	23	345	33	356	1.28e-122	362
MS.gene78715.t1	MTR_3g116080	51.576	349	158	5	3	345	5	348	1.90e-122	357
MS.gene78715.t1	MTR_4g094918	51.613	341	150	5	8	345	14	342	4.03e-122	357
MS.gene78715.t1	MTR_1g018840	51.412	354	159	7	5	346	11	363	5.91e-122	360
MS.gene78715.t1	MTR_1g018840	51.862	349	159	6	6	346	16	363	1.19e-121	359
MS.gene78715.t1	MTR_4g079800	52.923	325	136	6	28	344	29	344	2.54e-121	354
MS.gene78715.t1	MTR_3g102220	52.273	352	155	8	6	345	12	362	1.19e-120	357
MS.gene78715.t1	MTR_2g075830	53.779	344	144	7	5	343	6	339	1.55e-118	347
MS.gene78715.t1	MTR_4g079440	50.291	344	155	6	6	344	12	344	2.40e-117	344
MS.gene78715.t1	MTR_2g090890	49.713	348	163	5	1	346	1	338	7.04e-115	337
MS.gene78715.t1	MTR_4g080360	51.792	307	144	3	40	344	37	341	1.34e-112	332
MS.gene78715.t1	MTR_2g083930	46.903	339	169	5	10	344	12	343	3.34e-106	315
MS.gene78715.t1	MTR_7g078600	46.789	327	157	7	25	347	27	340	5.28e-100	300
MS.gene78715.t1	MTR_7g079230	45.665	346	173	7	1	343	1	334	5.71e-99	297
MS.gene78715.t1	MTR_3g112430	49.196	311	155	3	39	347	35	344	1.20e-98	300
MS.gene78715.t1	MTR_2g016180	44.582	323	161	11	32	345	43	356	3.97e-83	261
MS.gene78715.t1	MTR_2g016270	44.099	322	149	10	32	345	43	341	8.21e-83	260
MS.gene78715.t1	MTR_2g015850	45.302	298	157	5	48	344	71	363	2.26e-81	253
MS.gene78715.t1	MTR_4g065077	43.099	355	176	13	6	345	14	357	1.62e-75	238
MS.gene78715.t1	MTR_2g015920	40.116	344	186	12	27	361	41	373	2.66e-73	232
MS.gene78715.t1	MTR_4g125300	43.624	298	148	9	53	344	65	348	6.10e-72	228
MS.gene78715.t1	MTR_8g086470	41.714	350	172	12	6	345	30	357	1.04e-68	220
MS.gene78715.t1	MTR_2g015930	37.978	366	186	16	6	345	9	359	2.19e-65	211
MS.gene78715.t1	MTR_1g023210	39.799	299	152	9	54	336	66	352	8.37e-62	202
MS.gene78715.t1	MTR_2g067900	41.528	301	159	11	55	344	32	326	9.31e-62	201
MS.gene78715.t1	MTR_4g071060	37.793	299	157	8	54	336	63	348	1.36e-58	194
MS.gene78715.t1	MTR_2g064475	38.192	343	162	13	40	342	16	348	3.39e-58	192
MS.gene78715.t1	MTR_2g067560	40.123	324	153	15	40	338	16	323	8.87e-58	201
MS.gene78715.t1	MTR_1g023810	42.731	227	122	6	119	342	40	261	1.25e-56	186
MS.gene78715.t1	MTR_7g078630	43.369	279	110	10	40	304	706	950	6.11e-55	194
MS.gene78715.t1	MTR_2g066980	35.179	307	142	11	55	343	50	317	2.31e-52	176
MS.gene78715.t1	MTR_1g094135	31.672	341	198	10	15	336	34	358	4.21e-52	177
MS.gene78715.t1	MTR_2g015840	31.429	315	144	8	32	343	25	270	1.01e-43	152
MS.gene78715.t1	MTR_4g072790	34.615	286	148	7	54	336	63	312	7.48e-43	152
MS.gene78715.t1	MTR_7g078580	63.636	99	35	1	246	344	4	101	2.03e-42	144
MS.gene78715.t1	MTR_4g073660	34.021	291	147	10	54	336	62	315	2.94e-40	145
MS.gene78715.t1	MTR_4g072340	33.677	291	148	9	54	336	63	316	3.20e-40	145
MS.gene78715.t1	MTR_7g079130	63.736	91	33	0	254	344	7	97	1.76e-37	130
MS.gene78715.t1	MTR_2g047960	74.684	79	20	0	137	215	7	85	7.06e-35	124
MS.gene78715.t1	MTR_1g025450	41.379	174	78	7	127	296	54	207	6.12e-33	129
MS.gene78715.t1	MTR_2g016380	45.902	122	59	4	228	345	18	136	2.41e-28	111
MS.gene78715.t1	MTR_7g111060	26.812	276	166	11	86	336	66	330	3.00e-23	99.8
MS.gene78715.t1	MTR_7g111050	29.918	244	139	10	130	350	106	340	3.78e-22	96.3
MS.gene78715.t1	MTR_3g029560	32.075	212	115	8	139	344	49	237	2.06e-20	89.4
MS.gene78715.t1	MTR_7g079260	55.556	72	16	1	270	341	7	62	6.33e-20	84.3
MS.gene78715.t1	MTR_7g078310	37.671	146	77	4	75	217	110	244	2.13e-19	87.0
MS.gene78715.t1	MTR_8g087510	40.000	130	72	5	169	296	23	148	5.68e-18	80.9
MS.gene78715.t1	MTR_7g111050	28.643	199	119	7	130	307	101	297	1.86e-14	73.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78715.t1	AT5G50260	70.556	360	105	1	4	362	2	361	0.0	557
MS.gene78715.t1	AT3G48340	67.313	361	115	2	4	362	2	361	0.0	517
MS.gene78715.t1	AT3G48350	64.444	360	126	2	4	362	2	360	1.57e-177	497
MS.gene78715.t1	AT3G48350	65.942	276	92	2	5	278	2	277	1.32e-134	386
MS.gene78715.t1	AT1G20850	54.037	322	144	3	25	345	36	354	1.31e-121	355
MS.gene78715.t1	AT4G35350	51.003	349	161	4	3	345	9	353	4.57e-121	353
MS.gene78715.t1	AT5G43060	50.857	350	156	5	6	345	11	354	2.08e-118	351
MS.gene78715.t1	AT1G47128	49.568	347	164	4	6	346	13	354	2.08e-118	351
MS.gene78715.t1	AT3G19390	48.442	353	173	6	1	350	5	351	6.96e-116	344
MS.gene78715.t1	AT4G36880	51.368	329	149	6	32	351	41	367	4.41e-114	337
MS.gene78715.t1	AT5G45890	47.143	350	167	7	6	343	1	344	4.82e-114	335
MS.gene78715.t1	AT3G19400	51.911	314	141	7	39	347	43	351	4.92e-108	321
MS.gene78715.t1	AT1G06260	48.286	350	165	7	1	345	5	343	1.72e-106	316
MS.gene78715.t1	AT4G11310	46.797	359	170	8	3	347	4	355	9.79e-106	315
MS.gene78715.t1	AT4G11320	46.499	357	169	8	6	347	13	362	1.53e-103	310
MS.gene78715.t1	AT4G11320	46.499	357	169	8	6	347	13	362	1.53e-103	310
MS.gene78715.t1	AT1G09850	49.677	310	149	4	38	345	30	334	4.41e-100	303
MS.gene78715.t1	AT4G23520	46.835	316	158	6	39	350	46	355	5.49e-99	297
MS.gene78715.t1	AT2G27420	45.533	347	174	5	6	344	8	347	7.08e-99	297
MS.gene78715.t1	AT3G49340	44.118	340	183	5	9	344	4	340	1.75e-97	293
MS.gene78715.t1	AT2G34080	44.348	345	181	6	6	344	5	344	4.82e-94	285
MS.gene78715.t1	AT1G29090	44.722	360	168	11	1	344	10	354	5.99e-91	277
MS.gene78715.t1	AT3G43960	46.462	325	158	11	39	354	40	357	4.64e-89	273
MS.gene78715.t1	AT1G29080	41.311	351	193	8	1	344	1	345	1.30e-87	268
MS.gene78715.t1	AT4G35350	48.582	282	135	4	3	278	9	286	1.38e-86	263
MS.gene78715.t1	AT1G29110	39.031	351	189	9	1	344	1	333	2.78e-78	244
MS.gene78715.t1	AT3G19400	50.833	240	109	6	39	273	43	278	5.62e-73	229
MS.gene78715.t1	AT5G60360	41.333	300	156	9	51	344	71	356	8.53e-69	220
MS.gene78715.t1	AT3G45310	40.728	302	155	10	51	344	71	356	1.77e-66	214
MS.gene78715.t1	AT5G60360	41.000	300	156	10	51	344	71	355	3.04e-66	213
MS.gene78715.t1	AT3G45310	40.397	302	155	11	51	344	71	355	6.98e-64	207
MS.gene78715.t1	AT5G60360	40.206	291	154	9	51	335	71	347	1.27e-63	207
MS.gene78715.t1	AT2G21430	33.618	351	199	10	6	336	13	349	3.92e-60	198
MS.gene78715.t1	AT4G39090	36.789	299	161	8	54	336	66	352	1.04e-56	189
MS.gene78715.t1	AT3G54940	37.458	299	161	9	54	336	66	354	1.15e-54	184
MS.gene78715.t1	AT4G16190	37.248	298	161	9	55	336	71	358	6.26e-53	179
MS.gene78715.t1	AT3G54940	37.209	215	123	4	54	260	66	276	1.73e-38	139
MS.gene78715.t1	AT1G02305	27.596	337	209	11	50	362	33	358	1.88e-27	111
MS.gene78715.t1	AT4G01610	27.356	329	196	15	50	350	30	343	3.86e-23	99.4
MS.gene78715.t1	AT4G01610	26.748	329	198	15	50	350	30	343	2.85e-21	94.0
MS.gene78715.t1	AT2G22160	46.465	99	49	1	53	151	8	102	9.89e-21	86.7
MS.gene78715.t1	AT1G02300	31.381	239	132	10	147	362	146	375	1.22e-20	92.4

Find 90 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAGCCTATTTGTCTTTATT+TGG	0.139485	3.1:-62285091	None:intergenic
CCGTTGATGGAACTAATTAT+TGG	0.225728	3.1:+62285740	MS.gene78715:CDS
ATCAGATCCTCCGGCATCTA+TGG	0.300046	3.1:-62285474	None:intergenic
ACATGACCAACCATGAATTT+AGG	0.309976	3.1:+62284539	MS.gene78715:CDS
TTTGAGTTCATCAAGCAAAA+AGG	0.324145	3.1:+62285194	MS.gene78715:CDS
AAGCTCCTGCTTCTGTTGAT+TGG	0.324173	3.1:+62284653	MS.gene78715:CDS
GAACTCATGGGGAGCGGAAT+GGG	0.328427	3.1:+62285771	MS.gene78715:CDS
CCGAGGACACATCTTTATCT+TGG	0.334739	3.1:-62284348	None:intergenic
TAAACCAAATAAAGACAAAT+AGG	0.344680	3.1:+62285087	MS.gene78715:CDS
TGTCCTGTATAGGTAGTTGC+TGG	0.345293	3.1:+62285033	MS.gene78715:intron
GCATCTATGGCTACGGATAC+AGG	0.385812	3.1:-62285461	None:intergenic
TCAACTGTTGTAGCTGTTGA+AGG	0.388321	3.1:+62285062	MS.gene78715:CDS
TTGTTAAGTTTCAACTTGTA+AGG	0.389898	3.1:-62284508	None:intergenic
AGGATGCAATGGTGGGCTAA+TGG	0.392108	3.1:+62285163	MS.gene78715:CDS
GATCCATCATTTGCTGTGTA+AGG	0.397020	3.1:-62285242	None:intergenic
CACTTCCACGTGGCGTGCCT+CGG	0.411657	3.1:-62284603	None:intergenic
GATTGTTCTGTCCATTGCTT+TGG	0.412192	3.1:+62284297	MS.gene78715:CDS
ATCAACAGAAGCAGGAGCTT+TGG	0.424537	3.1:-62284650	None:intergenic
TGACCATCAATTGACACAGC+TGG	0.433204	3.1:-62285383	None:intergenic
GGTGTAGCAATAGTAGGATA+TGG	0.443941	3.1:+62285712	MS.gene78715:CDS
TGATGGTCATGAGACTGTTC+CGG	0.449890	3.1:+62285397	MS.gene78715:CDS
GGAGTCACCACACGGTTTCT+CGG	0.450148	3.1:+62284404	MS.gene78715:CDS
GGATATGGAACAACCGTTGA+TGG	0.454887	3.1:+62285727	MS.gene78715:CDS
GTCCCAGCTGTGTCAATTGA+TGG	0.458384	3.1:+62285380	MS.gene78715:CDS
CGAGGCACGCCACGTGGAAG+TGG	0.460548	3.1:+62284604	MS.gene78715:CDS
TCATCCTTGGTAGATGATAA+AGG	0.462977	3.1:-62285907	None:intergenic
TTTCTCCAATCAACAGAAGC+AGG	0.466214	3.1:-62284658	None:intergenic
TATCCATCTTGTTAGTGTTG+TGG	0.468486	3.1:-62284483	None:intergenic
CATAGGTAGTCCTAAATTCA+TGG	0.476124	3.1:-62284549	None:intergenic
ATTGGATTGTGAGGAACTCA+TGG	0.493389	3.1:+62285758	MS.gene78715:CDS
CTATTACAGGGAGTATTTAG+TGG	0.493552	3.1:+62285664	MS.gene78715:intron
AATCATGGTGTAGCAATAGT+AGG	0.495226	3.1:+62285706	MS.gene78715:CDS
GTCACTGAGGTCAAGGATCA+AGG	0.495703	3.1:+62284691	MS.gene78715:CDS
TCTAACAAGGAAGGACTTTG+TGG	0.498260	3.1:+62285829	MS.gene78715:CDS
GTAGATGATAAAGGTCCTGC+TGG	0.507201	3.1:-62285898	None:intergenic
AAACATTCAAAGCTCATCCT+TGG	0.508057	3.1:-62285920	None:intergenic
TACCCTTACACAGCAAATGA+TGG	0.512248	3.1:+62285239	MS.gene78715:CDS
GAATTTAGGACTACCTATGC+TGG	0.513702	3.1:+62284553	MS.gene78715:CDS
GGAACTCATGGGGAGCGGAA+TGG	0.524402	3.1:+62285770	MS.gene78715:CDS
GTCCTCGGACAAAAGCTTGT+GGG	0.525849	3.1:+62284363	MS.gene78715:CDS
AGGACTTTGTGGCATAGCTA+TGG	0.526821	3.1:+62285840	MS.gene78715:CDS
TGTGACAATCAAGAAAATCA+AGG	0.527505	3.1:+62285143	MS.gene78715:CDS
GAAAATCAAGGATGCAATGG+TGG	0.535112	3.1:+62285155	MS.gene78715:CDS
GTCCTGTATAGGTAGTTGCT+GGG	0.537057	3.1:+62285034	MS.gene78715:intron
TCCTCCGGCATCTATGGCTA+CGG	0.543132	3.1:-62285468	None:intergenic
GACTACCTATGCTGGATCAA+AGG	0.546319	3.1:+62284561	MS.gene78715:CDS
CAAGAAAATCAAGGATGCAA+TGG	0.548537	3.1:+62285152	MS.gene78715:CDS
AAACTGGAAATCAGATCCTC+CGG	0.552551	3.1:-62285483	None:intergenic
TCTGTTGATTGGAGAAAGAA+AGG	0.553167	3.1:+62284664	MS.gene78715:CDS
GGCTACGGATACAGGTTGGT+TGG	0.553342	3.1:-62285453	None:intergenic
AGGTGCTGTCACTGAGGTCA+AGG	0.558185	3.1:+62284684	MS.gene78715:CDS
ATCCATCATTTGCTGTGTAA+GGG	0.560080	3.1:-62285241	None:intergenic
TGTCCACAACACTAACAAGA+TGG	0.563716	3.1:+62284480	MS.gene78715:CDS
CCAAGATAAAGATGTGTCCT+CGG	0.565632	3.1:+62284348	MS.gene78715:CDS
GACCATCAATTGACACAGCT+GGG	0.568817	3.1:-62285382	None:intergenic
CCAATAATTAGTTCCATCAA+CGG	0.573198	3.1:-62285740	None:intergenic
TGTGGGACTTATACGAGAGA+TGG	0.573304	3.1:+62284380	MS.gene78715:CDS
TTGGATTGTGAGGAACTCAT+GGG	0.574375	3.1:+62285759	MS.gene78715:CDS
AACTCATGGGGAGCGGAATG+GGG	0.577276	3.1:+62285772	MS.gene78715:CDS
TGTGGTAAAGAGCTAAATCA+TGG	0.584208	3.1:+62285691	MS.gene78715:CDS
AAAGCATCTTCATCGTTAGC+CGG	0.584931	3.1:-62285416	None:intergenic
AAATTCATGGTTGGTCATGT+CGG	0.587621	3.1:-62284536	None:intergenic
GGAGCGGAATGGGGTGAACA+AGG	0.588520	3.1:+62285781	MS.gene78715:CDS
ATTAACCTTTGATCCAGCAT+AGG	0.590030	3.1:-62284566	None:intergenic
ATCTTGGAAATCGAAGCTCT+CGG	0.596119	3.1:-62284332	None:intergenic
TACTTCAAGAACCAAAGCAA+TGG	0.600914	3.1:-62284308	None:intergenic
TGTGAGGAACTCATGGGGAG+CGG	0.601438	3.1:+62285765	MS.gene78715:CDS
TGTCCTCGGACAAAAGCTTG+TGG	0.602219	3.1:+62284362	MS.gene78715:CDS
ATGTTCCGAGGCACGCCACG+TGG	0.610728	3.1:+62284598	MS.gene78715:CDS
GCGTGCCTCGGAACATTCTG+TGG	0.611727	3.1:-62284591	None:intergenic
AGGAATGTATCTAACAAGGA+AGG	0.612463	3.1:+62285820	MS.gene78715:CDS
GAACTAATTATTGGATTGTG+AGG	0.613342	3.1:+62285749	MS.gene78715:CDS
GGTAGTCCTAAATTCATGGT+TGG	0.613532	3.1:-62284545	None:intergenic
AAAATCAAGGATGCAATGGT+GGG	0.617377	3.1:+62285156	MS.gene78715:CDS
ATAAGCTCTTGTTCAGACAA+AGG	0.634536	3.1:-62285116	None:intergenic
GGAGTATTTAGTGGCGACTG+TGG	0.639104	3.1:+62285673	MS.gene78715:CDS
AAGGTTACATCAGAATGCAA+AGG	0.641547	3.1:+62285800	MS.gene78715:CDS
GCAAAGGAATGTATCTAACA+AGG	0.642601	3.1:+62285816	MS.gene78715:CDS
CTATGGCTACGGATACAGGT+TGG	0.643007	3.1:-62285457	None:intergenic
GGGACTTATACGAGAGATGG+AGG	0.652822	3.1:+62284383	MS.gene78715:CDS
GTATCCGTAGCCATAGATGC+CGG	0.655334	3.1:+62285464	MS.gene78715:CDS
AGGACCTTTATCATCTACCA+AGG	0.670444	3.1:+62285903	MS.gene78715:CDS
AATCACCACAGAATGTTCCG+AGG	0.693791	3.1:+62284586	MS.gene78715:CDS
TCCGTAGCCATAGATGCCGG+AGG	0.697215	3.1:+62285467	MS.gene78715:CDS
GTCAAGGATCAAGGCCAATG+TGG	0.699707	3.1:+62284700	MS.gene78715:CDS
GAGATGGAGGAGTCACCACA+CGG	0.702988	3.1:+62284396	MS.gene78715:CDS
TGGATTGTGAGGAACTCATG+GGG	0.709984	3.1:+62285760	MS.gene78715:CDS
CAAGGTTCCGAGAAACCGTG+TGG	0.715039	3.1:-62284411	None:intergenic
AAAGAAAGGTGCTGTCACTG+AGG	0.722701	3.1:+62284678	MS.gene78715:CDS
ATGAATGTTCCACTTCCACG+TGG	0.756623	3.1:-62284613	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAAAATATATGATCT+TGG	-	chr3.1:62284960-62284979	None:intergenic	10.0%
!!!	TTTTTACTTTTTTATTTTGA+AGG	-	chr3.1:62285572-62285591	None:intergenic	10.0%
!!	TAAAAAAGTAAAAAAAGAGA+TGG	+	chr3.1:62285578-62285597	MS.gene78715:intron	15.0%
!!!	ATGAATAATTTTTGAATCAT+AGG	-	chr3.1:62285347-62285366	None:intergenic	15.0%
!!	TAAACCAAATAAAGACAAAT+AGG	+	chr3.1:62285087-62285106	MS.gene78715:CDS	20.0%
!	TATAATTGACATGCAACTTT+AGG	+	chr3.1:62285319-62285338	MS.gene78715:intron	25.0%
!	TCAAAAATTATTCATGTTGC+AGG	+	chr3.1:62285352-62285371	MS.gene78715:intron	25.0%
!	TTGTTAAGTTTCAACTTGTA+AGG	-	chr3.1:62284511-62284530	None:intergenic	25.0%
!!	ATTTTGTATACACCTATTAC+AGG	+	chr3.1:62285651-62285670	MS.gene78715:intron	25.0%
!!	TTTTGTATACACCTATTACA+GGG	+	chr3.1:62285652-62285671	MS.gene78715:intron	25.0%
!!!	ACTTTTTTAACTAACCACAT+TGG	-	chr3.1:62284717-62284736	None:intergenic	25.0%
	CCAATAATTAGTTCCATCAA+CGG	-	chr3.1:62285743-62285762	None:intergenic	30.0%
	CTAGCCTATTTGTCTTTATT+TGG	-	chr3.1:62285094-62285113	None:intergenic	30.0%
	GAACTAATTATTGGATTGTG+AGG	+	chr3.1:62285749-62285768	MS.gene78715:CDS	30.0%
	TGTGACAATCAAGAAAATCA+AGG	+	chr3.1:62285143-62285162	MS.gene78715:CDS	30.0%
	TTGTGATTAATGTCCTGTAT+AGG	+	chr3.1:62285023-62285042	MS.gene78715:intron	30.0%
!	TTTGAGTTCATCAAGCAAAA+AGG	+	chr3.1:62285194-62285213	MS.gene78715:CDS	30.0%
!	TTTTTACCTCTGAGTAAAAC+TGG	-	chr3.1:62285502-62285521	None:intergenic	30.0%
!!	TTTTGGAGGAGTTTTTAACT+GGG	-	chr3.1:62285876-62285895	None:intergenic	30.0%
!!	TTTTTGGAGGAGTTTTTAAC+TGG	-	chr3.1:62285877-62285896	None:intergenic	30.0%
	AAAATCAAGGATGCAATGGT+GGG	+	chr3.1:62285156-62285175	MS.gene78715:CDS	35.0%
	AAGGTTACATCAGAATGCAA+AGG	+	chr3.1:62285800-62285819	MS.gene78715:CDS	35.0%
	AATCATGGTGTAGCAATAGT+AGG	+	chr3.1:62285706-62285725	MS.gene78715:CDS	35.0%
	ACATGACCAACCATGAATTT+AGG	+	chr3.1:62284539-62284558	MS.gene78715:CDS	35.0%
	AGGAATGTATCTAACAAGGA+AGG	+	chr3.1:62285820-62285839	MS.gene78715:CDS	35.0%
	ATAAGCTCTTGTTCAGACAA+AGG	-	chr3.1:62285119-62285138	None:intergenic	35.0%
	ATTAACCTTTGATCCAGCAT+AGG	-	chr3.1:62284569-62284588	None:intergenic	35.0%
	ATTCTCTTGTGTCAATAGAG+AGG	-	chr3.1:62285536-62285555	None:intergenic	35.0%
	CAAGAAAATCAAGGATGCAA+TGG	+	chr3.1:62285152-62285171	MS.gene78715:CDS	35.0%
	CACTAAATACTCCCTGTAAT+AGG	-	chr3.1:62285666-62285685	None:intergenic	35.0%
	CATAGGTAGTCCTAAATTCA+TGG	-	chr3.1:62284552-62284571	None:intergenic	35.0%
	GACACCATCTAATCAAAGAT+CGG	+	chr3.1:62284787-62284806	MS.gene78715:intron	35.0%
	GCAAAGGAATGTATCTAACA+AGG	+	chr3.1:62285816-62285835	MS.gene78715:CDS	35.0%
	TACTTCAAGAACCAAAGCAA+TGG	-	chr3.1:62284311-62284330	None:intergenic	35.0%
	TATCCATCTTGTTAGTGTTG+TGG	-	chr3.1:62284486-62284505	None:intergenic	35.0%
	TCATCCTTGGTAGATGATAA+AGG	-	chr3.1:62285910-62285929	None:intergenic	35.0%
	TGTGGTAAAGAGCTAAATCA+TGG	+	chr3.1:62285691-62285710	MS.gene78715:CDS	35.0%
!	AAATTCATGGTTGGTCATGT+CGG	-	chr3.1:62284539-62284558	None:intergenic	35.0%
!	CCGTTGATGGAACTAATTAT+TGG	+	chr3.1:62285740-62285759	MS.gene78715:CDS	35.0%
!	TCTGTTGATTGGAGAAAGAA+AGG	+	chr3.1:62284664-62284683	MS.gene78715:CDS	35.0%
!	TGATTTCCAGTTTTACTCAG+AGG	+	chr3.1:62285493-62285512	MS.gene78715:CDS	35.0%
!!	ATCCATCATTTGCTGTGTAA+GGG	-	chr3.1:62285244-62285263	None:intergenic	35.0%
!!	CTATTACAGGGAGTATTTAG+TGG	+	chr3.1:62285664-62285683	MS.gene78715:intron	35.0%
	AAACTGGAAATCAGATCCTC+CGG	-	chr3.1:62285486-62285505	None:intergenic	40.0%
	ACTGTGTGAATGCAAAGTGA+TGG	-	chr3.1:62284758-62284777	None:intergenic	40.0%
	AGGACCTTTATCATCTACCA+AGG	+	chr3.1:62285903-62285922	MS.gene78715:CDS	40.0%
	ATCTTGGAAATCGAAGCTCT+CGG	-	chr3.1:62284335-62284354	None:intergenic	40.0%
	ATTGGATTGTGAGGAACTCA+TGG	+	chr3.1:62285758-62285777	MS.gene78715:CDS	40.0%
	CCAAGATAAAGATGTGTCCT+CGG	+	chr3.1:62284348-62284367	MS.gene78715:CDS	40.0%
	GAAAATCAAGGATGCAATGG+TGG	+	chr3.1:62285155-62285174	MS.gene78715:CDS	40.0%
	GAGTTCATCAAGCAAAAAGG+TGG	+	chr3.1:62285197-62285216	MS.gene78715:CDS	40.0%
	GATTGTTCTGTCCATTGCTT+TGG	+	chr3.1:62284297-62284316	MS.gene78715:CDS	40.0%
	GGTAGTCCTAAATTCATGGT+TGG	-	chr3.1:62284548-62284567	None:intergenic	40.0%
	GGTGTAGCAATAGTAGGATA+TGG	+	chr3.1:62285712-62285731	MS.gene78715:CDS	40.0%
	TACCCTTACACAGCAAATGA+TGG	+	chr3.1:62285239-62285258	MS.gene78715:CDS	40.0%
	TCAACTGTTGTAGCTGTTGA+AGG	+	chr3.1:62285062-62285081	MS.gene78715:CDS	40.0%
	TCGTCCGATCTTTGATTAGA+TGG	-	chr3.1:62284794-62284813	None:intergenic	40.0%
	TCTAACAAGGAAGGACTTTG+TGG	+	chr3.1:62285829-62285848	MS.gene78715:CDS	40.0%
	TGTCCACAACACTAACAAGA+TGG	+	chr3.1:62284480-62284499	MS.gene78715:CDS	40.0%
	TTGGATTGTGAGGAACTCAT+GGG	+	chr3.1:62285759-62285778	MS.gene78715:CDS	40.0%
	TTTCTCCAATCAACAGAAGC+AGG	-	chr3.1:62284661-62284680	None:intergenic	40.0%
!	AAAGCATCTTCATCGTTAGC+CGG	-	chr3.1:62285419-62285438	None:intergenic	40.0%
!	GAATTTAGGACTACCTATGC+TGG	+	chr3.1:62284553-62284572	MS.gene78715:CDS	40.0%
!	TAAAGGTCCTGCTGGATTTT+TGG	-	chr3.1:62285893-62285912	None:intergenic	40.0%
!!	GATCCATCATTTGCTGTGTA+AGG	-	chr3.1:62285245-62285264	None:intergenic	40.0%
	AACTCCTCCAAAAATCCAGC+AGG	+	chr3.1:62285883-62285902	MS.gene78715:CDS	45.0%
	AAGCTCCTGCTTCTGTTGAT+TGG	+	chr3.1:62284653-62284672	MS.gene78715:CDS	45.0%
	AATCACCACAGAATGTTCCG+AGG	+	chr3.1:62284586-62284605	MS.gene78715:CDS	45.0%
	AGGACTTTGTGGCATAGCTA+TGG	+	chr3.1:62285840-62285859	MS.gene78715:CDS	45.0%
	ATCAACAGAAGCAGGAGCTT+TGG	-	chr3.1:62284653-62284672	None:intergenic	45.0%
	ATGAATGTTCCACTTCCACG+TGG	-	chr3.1:62284616-62284635	None:intergenic	45.0%
	CCGAGGACACATCTTTATCT+TGG	-	chr3.1:62284351-62284370	None:intergenic	45.0%
	GACCATCAATTGACACAGCT+GGG	-	chr3.1:62285385-62285404	None:intergenic	45.0%
	GACTACCTATGCTGGATCAA+AGG	+	chr3.1:62284561-62284580	MS.gene78715:CDS	45.0%
	GGATATGGAACAACCGTTGA+TGG	+	chr3.1:62285727-62285746	MS.gene78715:CDS	45.0%
	GTAGATGATAAAGGTCCTGC+TGG	-	chr3.1:62285901-62285920	None:intergenic	45.0%
	GTCCTGTATAGGTAGTTGCT+GGG	+	chr3.1:62285034-62285053	MS.gene78715:intron	45.0%
	TGACCATCAATTGACACAGC+TGG	-	chr3.1:62285386-62285405	None:intergenic	45.0%
	TGATGGTCATGAGACTGTTC+CGG	+	chr3.1:62285397-62285416	MS.gene78715:CDS	45.0%
	TGGATTGTGAGGAACTCATG+GGG	+	chr3.1:62285760-62285779	MS.gene78715:CDS	45.0%
	TGTCCTGTATAGGTAGTTGC+TGG	+	chr3.1:62285033-62285052	MS.gene78715:intron	45.0%
	TGTGGGACTTATACGAGAGA+TGG	+	chr3.1:62284380-62284399	MS.gene78715:CDS	45.0%
!!	AAAGAAAGGTGCTGTCACTG+AGG	+	chr3.1:62284678-62284697	MS.gene78715:CDS	45.0%
!!	AGCGCTTTTGTTTCTCGTCA+AGG	-	chr3.1:62284432-62284451	None:intergenic	45.0%
!!	GGAGGAGTTTTTAACTGGGT+AGG	-	chr3.1:62285872-62285891	None:intergenic	45.0%
	AGCCCAGCAACTACCTATAC+AGG	-	chr3.1:62285039-62285058	None:intergenic	50.0%
	ATCAGATCCTCCGGCATCTA+TGG	-	chr3.1:62285477-62285496	None:intergenic	50.0%
	CTATGGCTACGGATACAGGT+TGG	-	chr3.1:62285460-62285479	None:intergenic	50.0%
	GCATCTATGGCTACGGATAC+AGG	-	chr3.1:62285464-62285483	None:intergenic	50.0%
	GGGACTTATACGAGAGATGG+AGG	+	chr3.1:62284383-62284402	MS.gene78715:CDS	50.0%
	GTATCCGTAGCCATAGATGC+CGG	+	chr3.1:62285464-62285483	MS.gene78715:CDS	50.0%
	GTCAAGGATCAAGGCCAATG+TGG	+	chr3.1:62284700-62284719	MS.gene78715:CDS	50.0%
	GTCACTGAGGTCAAGGATCA+AGG	+	chr3.1:62284691-62284710	MS.gene78715:CDS	50.0%
	GTCCCAGCTGTGTCAATTGA+TGG	+	chr3.1:62285380-62285399	MS.gene78715:CDS	50.0%
	GTCCTCGGACAAAAGCTTGT+GGG	+	chr3.1:62284363-62284382	MS.gene78715:CDS	50.0%
	TGTCCTCGGACAAAAGCTTG+TGG	+	chr3.1:62284362-62284381	MS.gene78715:CDS	50.0%
!	GGAGTATTTAGTGGCGACTG+TGG	+	chr3.1:62285673-62285692	MS.gene78715:CDS	50.0%
!!	AGGATGCAATGGTGGGCTAA+TGG	+	chr3.1:62285163-62285182	MS.gene78715:CDS	50.0%
!!	AGGTCCTGCTGGATTTTTGG+AGG	-	chr3.1:62285890-62285909	None:intergenic	50.0%
	AACTCATGGGGAGCGGAATG+GGG	+	chr3.1:62285772-62285791	MS.gene78715:CDS	55.0%
	CAAGGTTCCGAGAAACCGTG+TGG	-	chr3.1:62284414-62284433	None:intergenic	55.0%
	GAACTCATGGGGAGCGGAAT+GGG	+	chr3.1:62285771-62285790	MS.gene78715:CDS	55.0%
	GAGATGGAGGAGTCACCACA+CGG	+	chr3.1:62284396-62284415	MS.gene78715:CDS	55.0%
	GGAGTCACCACACGGTTTCT+CGG	+	chr3.1:62284404-62284423	MS.gene78715:CDS	55.0%
	TCCTCCGGCATCTATGGCTA+CGG	-	chr3.1:62285471-62285490	None:intergenic	55.0%
	TGTGAGGAACTCATGGGGAG+CGG	+	chr3.1:62285765-62285784	MS.gene78715:CDS	55.0%
!	AGGTGCTGTCACTGAGGTCA+AGG	+	chr3.1:62284684-62284703	MS.gene78715:CDS	55.0%
!	GTCCCACAAGCTTTTGTCCG+AGG	-	chr3.1:62284368-62284387	None:intergenic	55.0%
!!	GGCTACGGATACAGGTTGGT+TGG	-	chr3.1:62285456-62285475	None:intergenic	55.0%
	GCGTGCCTCGGAACATTCTG+TGG	-	chr3.1:62284594-62284613	None:intergenic	60.0%
	GGAACTCATGGGGAGCGGAA+TGG	+	chr3.1:62285770-62285789	MS.gene78715:CDS	60.0%
	GGAGCGGAATGGGGTGAACA+AGG	+	chr3.1:62285781-62285800	MS.gene78715:CDS	60.0%
	TCCGTAGCCATAGATGCCGG+AGG	+	chr3.1:62285467-62285486	MS.gene78715:CDS	60.0%
	ATGTTCCGAGGCACGCCACG+TGG	+	chr3.1:62284598-62284617	MS.gene78715:CDS	65.0%
	CACTTCCACGTGGCGTGCCT+CGG	-	chr3.1:62284606-62284625	None:intergenic	65.0%
	CGAGGCACGCCACGTGGAAG+TGG	+	chr3.1:62284604-62284623	MS.gene78715:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	62284274	62285936	62284274	ID=MS.gene78715
chr3.1	mRNA	62284274	62285936	62284274	ID=MS.gene78715.t1;Parent=MS.gene78715
chr3.1	exon	62284274	62284721	62284274	ID=MS.gene78715.t1.exon1;Parent=MS.gene78715.t1
chr3.1	CDS	62284274	62284721	62284274	ID=cds.MS.gene78715.t1;Parent=MS.gene78715.t1
chr3.1	exon	62285045	62285280	62285045	ID=MS.gene78715.t1.exon2;Parent=MS.gene78715.t1
chr3.1	CDS	62285045	62285280	62285045	ID=cds.MS.gene78715.t1;Parent=MS.gene78715.t1
chr3.1	exon	62285374	62285514	62285374	ID=MS.gene78715.t1.exon3;Parent=MS.gene78715.t1
chr3.1	CDS	62285374	62285514	62285374	ID=cds.MS.gene78715.t1;Parent=MS.gene78715.t1
chr3.1	exon	62285673	62285936	62285673	ID=MS.gene78715.t1.exon4;Parent=MS.gene78715.t1
chr3.1	CDS	62285673	62285936	62285673	ID=cds.MS.gene78715.t1;Parent=MS.gene78715.t1

Gene Sequence

>MS.gene78715

ATGGAAACTAAGAAACTATTGTTGATTGTTCTGTCCATTGCTTTGGTTCTTGAAGTATCCGAGAGCTTCGATTTCCAAGATAAAGATGTGTCCTCGGACAAAAGCTTGTGGGACTTATACGAGAGATGGAGGAGTCACCACACGGTTTCTCGGAACCTTGACGAGAAACAAAAGCGCTTTAACGTGTTCAAATCAAATGTGATACATGTCCACAACACTAACAAGATGGATAAGCCTTACAAGTTGAAACTTAACAAGTTTGCCGACATGACCAACCATGAATTTAGGACTACCTATGCTGGATCAAAGGTTAATCACCACAGAATGTTCCGAGGCACGCCACGTGGAAGTGGAACATTCATGTATGAAAATTTTACCAAAGCTCCTGCTTCTGTTGATTGGAGAAAGAAAGGTGCTGTCACTGAGGTCAAGGATCAAGGCCAATGTGGTTAGTTAAAAAAGTTATTATTGAGAATCTTCACCATCACTTTGCATTCACACAGTCAACAGTTTGACACCATCTAATCAAAGATCGGACGACTCTTTGTTACGAGCAAATTTTTTAAATATTTAGAAATTCAATTCACTTACTGACTACATAAATGCATATGATTTCTGACTGCAAGTCAATCTATTTTCAAATAGTTATACTAGTCATTATTAATCATATTCATAGAGCTTAACCAAGATCATATATTTTTTTTTTTTTTCACACTATTATGCAATATAGCTTTTTATAACATTGTTGTTTGTGATTAATGTCCTGTATAGGTAGTTGCTGGGCTTTTTCAACTGTTGTAGCTGTTGAAGGCATAAACCAAATAAAGACAAATAGGCTAGTGCCTTTGTCTGAACAAGAGCTTATAGATTGTGACAATCAAGAAAATCAAGGATGCAATGGTGGGCTAATGGAATATGCTTTTGAGTTCATCAAGCAAAAAGGTGGCATAACAACAGAAAGCTATTACCCTTACACAGCAAATGATGGATCATGTGATGCAACAAAAGTGAGTAAAGATGCTAAACTTCTATTGAAATTTTTTAATATAATTGACATGCAACTTTAGGAACCTATGATTCAAAAATTATTCATGTTGCAGGAAAATGTCCCAGCTGTGTCAATTGATGGTCATGAGACTGTTCCGGCTAACGATGAAGATGCTTTGCTCAAAGCTGTTGCCAACCAACCTGTATCCGTAGCCATAGATGCCGGAGGATCTGATTTCCAGTTTTACTCAGAGGTAAAAATGAGAATGAATCCTCTCTATTGACACAAGAGAATAATGCAATCTAAACCTTCAAAATAAAAAAGTAAAAAAAGAGATGGAAGAAATATGCTATTATTAGCAATCAATTTTGAAACTAATGTGTATCTTAATTTTGTATACACCTATTACAGGGAGTATTTAGTGGCGACTGTGGTAAAGAGCTAAATCATGGTGTAGCAATAGTAGGATATGGAACAACCGTTGATGGAACTAATTATTGGATTGTGAGGAACTCATGGGGAGCGGAATGGGGTGAACAAGGTTACATCAGAATGCAAAGGAATGTATCTAACAAGGAAGGACTTTGTGGCATAGCTATGGAAGCTTCCTACCCAGTTAAAAACTCCTCCAAAAATCCAGCAGGACCTTTATCATCTACCAAGGATGAGCTTTGA

Protein sequence

>MS.gene78715.t1

METKKLLLIVLSIALVLEVSESFDFQDKDVSSDKSLWDLYERWRSHHTVSRNLDEKQKRFNVFKSNVIHVHNTNKMDKPYKLKLNKFADMTNHEFRTTYAGSKVNHHRMFRGTPRGSGTFMYENFTKAPASVDWRKKGAVTEVKDQGQCGSCWAFSTVVAVEGINQIKTNRLVPLSEQELIDCDNQENQGCNGGLMEYAFEFIKQKGGITTESYYPYTANDGSCDATKENVPAVSIDGHETVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFSGDCGKELNHGVAIVGYGTTVDGTNYWIVRNSWGAEWGEQGYIRMQRNVSNKEGLCGIAMEASYPVKNSSKNPAGPLSSTKDEL