AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011363

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011363.t1 MTR_4g104370 96.944 360 11 0 1 360 1 360 0.0 736
MS.gene011363.t1 MTR_3g075390 74.309 362 91 2 1 360 1 362 0.0 578
MS.gene011363.t1 MTR_4g079770 55.172 348 145 8 1 342 1 343 4.59e-129 374
MS.gene011363.t1 MTR_4g107930 54.467 347 149 6 1 342 1 343 1.98e-128 372
MS.gene011363.t1 MTR_5g022560 55.102 343 143 9 5 342 6 342 1.18e-127 370
MS.gene011363.t1 MTR_4g079470 58.176 318 123 7 30 342 31 343 3.81e-127 369
MS.gene011363.t1 MTR_4g047610 59.425 313 115 7 38 342 33 341 1.30e-126 367
MS.gene011363.t1 MTR_4g079800 57.547 318 125 7 30 342 31 343 1.13e-125 365
MS.gene011363.t1 MTR_4g080700 57.692 312 120 8 35 342 35 338 1.01e-122 357
MS.gene011363.t1 MTR_4g080730 56.782 317 125 8 30 342 30 338 1.12e-122 357
MS.gene011363.t1 MTR_3g116080 52.874 348 152 6 5 345 7 349 2.20e-122 357
MS.gene011363.t1 MTR_2g075830 54.942 344 139 9 5 342 6 339 8.42e-121 352
MS.gene011363.t1 MTR_1g107550 54.711 329 139 7 23 345 33 357 7.15e-118 350
MS.gene011363.t1 MTR_1g018840 50.704 355 162 8 1 344 10 362 2.10e-116 345
MS.gene011363.t1 MTR_1g018840 50.704 355 162 8 1 344 10 362 6.11e-116 345
MS.gene011363.t1 MTR_4g094918 49.425 348 163 7 1 344 4 342 1.98e-115 339
MS.gene011363.t1 MTR_4g079440 54.369 309 126 6 40 343 46 344 6.17e-115 338
MS.gene011363.t1 MTR_3g102220 50.716 349 161 8 6 345 17 363 4.01e-113 337
MS.gene011363.t1 MTR_4g080360 50.000 314 152 4 33 343 30 341 1.90e-109 323
MS.gene011363.t1 MTR_2g090890 51.948 308 136 6 40 344 39 337 7.56e-107 317
MS.gene011363.t1 MTR_2g083930 48.094 341 159 7 11 343 13 343 1.49e-106 316
MS.gene011363.t1 MTR_7g078600 49.845 323 142 10 26 343 30 337 7.36e-101 301
MS.gene011363.t1 MTR_3g112430 48.875 311 155 4 39 346 35 344 1.46e-95 291
MS.gene011363.t1 MTR_7g079230 47.006 334 160 8 13 342 14 334 2.40e-94 285
MS.gene011363.t1 MTR_2g015850 46.465 297 154 4 48 343 71 363 1.18e-84 261
MS.gene011363.t1 MTR_2g016180 43.143 350 179 12 8 345 16 357 1.82e-84 265
MS.gene011363.t1 MTR_2g016270 42.571 350 166 12 8 345 16 342 1.21e-83 262
MS.gene011363.t1 MTR_4g065077 40.223 358 181 15 6 344 14 357 2.47e-69 221
MS.gene011363.t1 MTR_2g067900 41.194 335 174 14 22 343 2 326 1.26e-67 216
MS.gene011363.t1 MTR_2g015920 37.168 339 189 12 30 355 43 370 7.78e-66 213
MS.gene011363.t1 MTR_4g125300 42.525 301 148 11 52 343 64 348 3.51e-65 210
MS.gene011363.t1 MTR_2g067560 42.271 317 155 14 40 337 16 323 4.66e-64 219
MS.gene011363.t1 MTR_8g086470 39.377 353 175 14 6 344 30 357 5.87e-64 207
MS.gene011363.t1 MTR_2g015930 37.879 330 178 14 30 344 42 359 5.43e-61 200
MS.gene011363.t1 MTR_1g023210 39.073 302 149 12 54 335 66 352 3.16e-59 195
MS.gene011363.t1 MTR_2g064475 35.965 342 170 13 40 341 16 348 2.02e-56 188
MS.gene011363.t1 MTR_1g023810 42.035 226 122 6 120 341 41 261 3.00e-56 185
MS.gene011363.t1 MTR_4g071060 37.377 305 159 10 49 335 58 348 2.17e-55 185
MS.gene011363.t1 MTR_1g094135 34.111 343 186 11 15 335 34 358 2.24e-54 183
MS.gene011363.t1 MTR_2g066980 36.025 322 149 12 40 342 34 317 2.90e-54 181
MS.gene011363.t1 MTR_7g078630 38.411 302 137 10 19 306 687 953 1.85e-52 187
MS.gene011363.t1 MTR_7g078580 63.636 99 35 1 245 343 4 101 5.81e-41 140
MS.gene011363.t1 MTR_4g072790 36.986 292 142 13 49 335 58 312 1.11e-40 146
MS.gene011363.t1 MTR_4g072340 34.915 295 148 12 49 335 58 316 3.68e-38 139
MS.gene011363.t1 MTR_7g079130 65.217 92 32 0 252 343 6 97 4.16e-38 132
MS.gene011363.t1 MTR_4g073660 34.915 295 148 12 49 335 57 315 4.44e-38 139
MS.gene011363.t1 MTR_1g025450 40.678 177 80 7 124 295 51 207 7.64e-34 131
MS.gene011363.t1 MTR_2g015840 50.450 111 54 1 232 342 161 270 2.86e-32 122
MS.gene011363.t1 MTR_2g015840 35.256 156 94 5 11 163 5 156 4.11e-20 89.4
MS.gene011363.t1 MTR_2g016380 43.902 123 62 4 227 345 18 137 3.10e-27 108
MS.gene011363.t1 MTR_2g047960 65.385 78 26 1 138 214 8 85 6.03e-26 100
MS.gene011363.t1 MTR_3g029560 32.075 212 114 6 139 343 49 237 1.83e-23 97.8
MS.gene011363.t1 MTR_7g111060 27.354 223 136 7 134 335 113 330 3.01e-22 96.7
MS.gene011363.t1 MTR_7g111050 27.984 243 147 8 126 345 102 339 5.59e-22 95.9
MS.gene011363.t1 MTR_7g079260 56.944 72 15 1 269 340 7 62 1.89e-20 85.9
MS.gene011363.t1 MTR_7g078310 38.356 146 75 5 75 216 110 244 1.27e-18 84.7
MS.gene011363.t1 MTR_7g111050 26.238 202 127 6 126 306 97 297 3.04e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011363.t1 AT5G50260 69.529 361 107 2 3 360 1 361 0.0 535
MS.gene011363.t1 AT3G48340 65.470 362 120 4 3 360 1 361 3.45e-172 483
MS.gene011363.t1 AT3G48350 62.778 360 132 2 3 360 1 360 1.77e-168 474
MS.gene011363.t1 AT3G48350 61.733 277 104 2 3 277 1 277 4.26e-122 354
MS.gene011363.t1 AT1G20850 53.106 322 146 4 25 344 36 354 4.85e-118 346
MS.gene011363.t1 AT4G35350 49.856 347 159 6 6 344 14 353 1.95e-116 342
MS.gene011363.t1 AT5G45890 49.292 353 152 9 3 342 6 344 7.40e-116 340
MS.gene011363.t1 AT5G43060 51.657 362 151 8 1 345 1 355 4.60e-115 342
MS.gene011363.t1 AT3G19390 50.000 354 162 8 1 349 8 351 2.38e-114 340
MS.gene011363.t1 AT1G06260 48.974 341 164 6 7 344 10 343 2.48e-111 328
MS.gene011363.t1 AT1G47128 52.997 317 140 4 32 344 42 353 2.51e-111 333
MS.gene011363.t1 AT4G36880 49.547 331 155 7 32 352 41 369 9.33e-109 323
MS.gene011363.t1 AT2G27420 49.062 320 154 5 33 343 28 347 1.03e-100 301
MS.gene011363.t1 AT3G19400 47.463 335 161 9 18 346 26 351 3.17e-100 301
MS.gene011363.t1 AT4G11310 44.413 358 177 10 4 346 5 355 5.86e-99 298
MS.gene011363.t1 AT4G11320 48.243 313 152 7 39 346 55 362 1.15e-98 297
MS.gene011363.t1 AT4G11320 48.243 313 152 7 39 346 55 362 1.15e-98 297
MS.gene011363.t1 AT3G49340 47.468 316 158 6 33 343 28 340 3.65e-98 295
MS.gene011363.t1 AT2G34080 45.322 342 181 5 6 343 5 344 7.22e-98 294
MS.gene011363.t1 AT4G23520 45.886 316 160 7 39 349 46 355 6.99e-96 290
MS.gene011363.t1 AT1G09850 48.387 310 152 6 38 344 30 334 6.84e-94 287
MS.gene011363.t1 AT1G29090 43.966 348 187 6 2 343 9 354 1.06e-92 281
MS.gene011363.t1 AT1G29080 45.031 322 164 9 30 343 29 345 4.79e-88 269
MS.gene011363.t1 AT4G35350 47.687 281 130 6 7 277 13 286 5.02e-83 254
MS.gene011363.t1 AT3G43960 42.319 345 177 13 13 347 18 350 2.42e-77 243
MS.gene011363.t1 AT1G29110 38.551 345 186 10 7 343 7 333 3.45e-74 233
MS.gene011363.t1 AT3G19400 45.594 261 128 8 18 272 26 278 7.68e-67 213
MS.gene011363.t1 AT5G60360 41.196 301 154 11 51 343 71 356 8.01e-64 207
MS.gene011363.t1 AT3G45310 41.196 301 154 11 51 343 71 356 5.94e-63 205
MS.gene011363.t1 AT5G60360 40.864 301 154 12 51 343 71 355 2.50e-61 201
MS.gene011363.t1 AT3G45310 40.864 301 154 12 51 343 71 355 2.68e-60 198
MS.gene011363.t1 AT5G60360 40.411 292 151 11 51 334 71 347 4.69e-59 195
MS.gene011363.t1 AT4G39090 38.511 309 161 9 44 335 56 352 1.20e-58 194
MS.gene011363.t1 AT2G21430 35.112 356 186 14 6 335 13 349 4.85e-58 192
MS.gene011363.t1 AT4G16190 36.700 297 163 8 55 335 71 358 1.82e-52 178
MS.gene011363.t1 AT3G54940 35.667 300 164 10 54 335 66 354 8.00e-51 174
MS.gene011363.t1 AT3G54940 35.185 216 125 6 54 259 66 276 2.99e-35 130
MS.gene011363.t1 AT1G02305 28.105 306 174 13 85 360 68 357 8.86e-26 106
MS.gene011363.t1 AT4G01610 25.357 280 172 10 85 338 65 333 1.70e-21 94.7
MS.gene011363.t1 AT1G02300 29.114 237 137 8 147 360 146 374 4.39e-21 93.6
MS.gene011363.t1 AT2G22160 50.000 96 36 2 60 151 15 102 8.65e-21 86.7
MS.gene011363.t1 AT4G01610 24.643 280 174 10 85 338 65 333 1.61e-19 89.0

Find 88 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGGCTTATCTAATTTAT+TGG 0.217778 8.4:-21598888 None:intergenic
ACATGACAAATTATGAATTT+AGG 0.245011 8.4:+21598936 MS.gene011363:CDS
TTATTCAACTTAAGCTTATA+AGG 0.250519 8.4:-21598905 None:intergenic
AGCTAAGAGCTTTGATTTCC+AGG 0.263284 8.4:+21598727 MS.gene011363:CDS
AGGATGCAACGGTGGGTTAA+TGG 0.279883 8.4:+21599503 MS.gene011363:CDS
TAACTTGTATAGGTAGTTGC+TGG 0.293976 8.4:+21599373 MS.gene011363:intron
ATCATTTATAGCTCATCTTT+TGG 0.315152 8.4:-21600221 None:intergenic
CTCAAGATGGAACAAAATAT+TGG 0.333579 8.4:+21600045 MS.gene011363:CDS
GAGCATCTATGCTGATTCTA+AGG 0.335794 8.4:+21598958 MS.gene011363:CDS
GGGAAGTGAATGGGGAGAAC+AGG 0.351413 8.4:+21600085 MS.gene011363:CDS
TAATTACAGGGAGTATTTAC+TGG 0.363002 8.4:+21599969 MS.gene011363:intron
TCGATTGATTGGAGGAAGAT+AGG 0.372632 8.4:+21599061 MS.gene011363:CDS
TTCGAGTTCATCAAGCAAAA+TGG 0.391365 8.4:+21599534 MS.gene011363:CDS
TCAACAATTGTAGCTGTTGA+AGG 0.396428 8.4:+21599402 MS.gene011363:CDS
ATGTTCCTTCTTCGATTGAT+TGG 0.402034 8.4:+21599050 MS.gene011363:CDS
ATTGGACAGTGAAGAACTCT+TGG 0.427329 8.4:+21600063 MS.gene011363:CDS
AAACCAGCAGTGTCAATTGA+TGG 0.433654 8.4:+21599741 MS.gene011363:CDS
GAACTCTTGGGGAAGTGAAT+GGG 0.438030 8.4:+21600076 MS.gene011363:CDS
TATCTAATTTATTGGTATTG+TGG 0.443235 8.4:-21598880 None:intergenic
GCATCAATAGCTACAGAAAT+CGG 0.448717 8.4:-21599822 None:intergenic
AATGCTGCTTCATTGTTAGC+AGG 0.455028 8.4:-21599777 None:intergenic
GGGGTAGCAATTGTGGGGTA+TGG 0.459702 8.4:+21600017 MS.gene011363:CDS
TAAAATCAAAGCTAGGGATA+AGG 0.466804 8.4:-21598699 None:intergenic
AGGTGCTGTCACCGGGGTAA+AGG 0.468205 8.4:+21599081 MS.gene011363:CDS
GGAAGATAGGTGCTGTCACC+GGG 0.470663 8.4:+21599074 MS.gene011363:CDS
ATTTCTGTAGCTATTGATGC+CGG 0.473128 8.4:+21599825 MS.gene011363:CDS
TGGCCATCAATTGACACTGC+TGG 0.473744 8.4:-21599744 None:intergenic
AGCATCAGAGAAAAGTTTGT+GGG 0.476908 8.4:+21598760 MS.gene011363:CDS
GAATCATGGGGTAGCAATTG+TGG 0.479525 8.4:+21600010 MS.gene011363:CDS
TTGGACAGTGAAGAACTCTT+GGG 0.479993 8.4:+21600064 MS.gene011363:CDS
AATCATGGGGTAGCAATTGT+GGG 0.482862 8.4:+21600011 MS.gene011363:CDS
TTTCTGTAGCTATTGATGCC+GGG 0.483109 8.4:+21599826 MS.gene011363:CDS
AGAACTCTTGGGGAAGTGAA+TGG 0.483270 8.4:+21600075 MS.gene011363:CDS
TTATCCTTATGCTGCAAAAG+AGG 0.485453 8.4:+21599575 MS.gene011363:CDS
TGTGGGATTTGTACGAGAGA+TGG 0.486184 8.4:+21598777 MS.gene011363:CDS
AACTTGTATAGGTAGTTGCT+GGG 0.493124 8.4:+21599374 MS.gene011363:intron
GGGTTACATTAGAATGCAAA+GGG 0.493549 8.4:+21600106 MS.gene011363:CDS
CTATTCCTAAAATCAAAGCT+AGG 0.494841 8.4:-21598706 None:intergenic
GGAAGTGAATGGGGAGAACA+GGG 0.501956 8.4:+21600086 MS.gene011363:CDS
AGGTCTATGTGGCATAGCTA+TGG 0.507498 8.4:+21600145 MS.gene011363:CDS
GGGAACCTGTAATATACAAA+AGG 0.508036 8.4:+21599596 MS.gene011363:CDS
TTCCTTCTTCGATTGATTGG+AGG 0.508300 8.4:+21599053 MS.gene011363:CDS
TTCTGTAGCTATTGATGCCG+GGG 0.509636 8.4:+21599827 MS.gene011363:CDS
TATCCTTATGCTGCAAAAGA+GGG 0.511600 8.4:+21599576 MS.gene011363:CDS
AGCTACAGAAATCGGTTGGT+TGG 0.518524 8.4:-21599814 None:intergenic
AGGAAGATAGGTGCTGTCAC+CGG 0.527243 8.4:+21599073 MS.gene011363:CDS
AACAAGTAAGCTTACCGCAT+TGG 0.533561 8.4:-21599111 None:intergenic
TGTGGTACTGAGCTGAATCA+TGG 0.537115 8.4:+21599996 MS.gene011363:CDS
TTCCTCCAATCAATCGAAGA+AGG 0.537545 8.4:-21599055 None:intergenic
CACAAGTTCTTGCTCGGACA+AGG 0.537976 8.4:-21599457 None:intergenic
TATTCCTAAAATCAAAGCTA+GGG 0.538115 8.4:-21598705 None:intergenic
CTGATGCTAAGTCATTCTCC+TGG 0.541617 8.4:-21598745 None:intergenic
GTGGTACTGAGCTGAATCAT+GGG 0.550981 8.4:+21599997 MS.gene011363:CDS
TGCGACACTAAAGTGAATCA+AGG 0.551630 8.4:+21599483 MS.gene011363:CDS
GGGTATGGAGCAACTCAAGA+TGG 0.553677 8.4:+21600032 MS.gene011363:CDS
GTCACCGGGGTAAAGGATCA+AGG 0.557892 8.4:+21599088 MS.gene011363:CDS
AACTCTTGGGGAAGTGAATG+GGG 0.569712 8.4:+21600077 MS.gene011363:CDS
GCAATCCACAAGTTCTTGCT+CGG 0.579328 8.4:-21599463 None:intergenic
ATGCTGCTTCATTGTTAGCA+GGG 0.581774 8.4:-21599776 None:intergenic
TGTTAGCAGGGACATTCTCA+TGG 0.582058 8.4:-21599764 None:intergenic
TAGCATCAGAGAAAAGTTTG+TGG 0.583867 8.4:+21598759 MS.gene011363:CDS
CATTGTCATGTAACATGCCA+CGG 0.592735 8.4:-21599000 None:intergenic
AAACTGGAAATCAGATCCCC+CGG 0.593442 8.4:-21599844 None:intergenic
GTGAATCAAGGATGCAACGG+TGG 0.599603 8.4:+21599495 MS.gene011363:CDS
AGGGTTACATTAGAATGCAA+AGG 0.601301 8.4:+21600105 MS.gene011363:CDS
CGTGGCATGTTACATGACAA+TGG 0.601342 8.4:+21599001 MS.gene011363:CDS
TCTATGTGGCATAGCTATGG+AGG 0.601740 8.4:+21600148 MS.gene011363:CDS
CAATAGCTACAGAAATCGGT+TGG 0.603005 8.4:-21599818 None:intergenic
CTTGTCCGAGCAAGAACTTG+TGG 0.608838 8.4:+21599458 MS.gene011363:CDS
TCTCACAAGCAAGGTCTATG+TGG 0.620594 8.4:+21600134 MS.gene011363:CDS
TGAATCAAGGATGCAACGGT+GGG 0.628142 8.4:+21599496 MS.gene011363:CDS
TCTGTAGCTATTGATGCCGG+GGG 0.632927 8.4:+21599828 MS.gene011363:CDS
TTGGCCTTGATCCTTTACCC+CGG 0.635267 8.4:-21599092 None:intergenic
TCCACATTCTCATACATGAA+CGG 0.636776 8.4:-21599025 None:intergenic
ACCGTTCATGTATGAGAATG+TGG 0.645392 8.4:+21599024 MS.gene011363:CDS
AAAGTGAATCAAGGATGCAA+CGG 0.646923 8.4:+21599492 MS.gene011363:CDS
AGGGGCATATCTCACAAGCA+AGG 0.659313 8.4:+21600125 MS.gene011363:CDS
TGGACAGTGAAGAACTCTTG+GGG 0.663115 8.4:+21600065 MS.gene011363:CDS
ATTGTGGACATGCATAACAT+TGG 0.668124 8.4:-21598864 None:intergenic
AATCATCATAGAATGTTCCG+TGG 0.676558 8.4:+21598983 MS.gene011363:CDS
ATCATGGGGTAGCAATTGTG+GGG 0.693047 8.4:+21600012 MS.gene011363:CDS
CAAGACTTCGAGTAACTGTG+TGG 0.728098 8.4:-21598808 None:intergenic
GGAGTATTTACTGGACACTG+TGG 0.740210 8.4:+21599978 MS.gene011363:CDS
TGGTACTGAGCTGAATCATG+GGG 0.741700 8.4:+21599998 MS.gene011363:CDS
GAAGATAGGTGCTGTCACCG+GGG 0.757764 8.4:+21599075 MS.gene011363:CDS
GTTCATGTATGAGAATGTGG+AGG 0.769545 8.4:+21599027 MS.gene011363:CDS
GGTTACATTAGAATGCAAAG+GGG 0.794482 8.4:+21600107 MS.gene011363:CDS
GTAAAGGATCAAGGCCAATG+CGG 0.813840 8.4:+21599097 MS.gene011363:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTATTAATTATATATT+AGG - chr8.4:21599654-21599673 None:intergenic 0.0%
!! AATTACAAAAATATAATTAC+AGG + chr8.4:21599956-21599975 MS.gene011363:intron 10.0%
!! ATTACAAAAATATAATTACA+GGG + chr8.4:21599957-21599976 MS.gene011363:intron 10.0%
!!! AATTTTATTTGATTAACTTA+TGG + chr8.4:21599669-21599688 MS.gene011363:intron 10.0%
!!! ATATTTTTGTAATTAAAAGT+GGG - chr8.4:21599950-21599969 None:intergenic 10.0%
!!! ATTTTATTTGATTAACTTAT+GGG + chr8.4:21599670-21599689 MS.gene011363:intron 10.0%
!!! TATATTTTTGTAATTAAAAG+TGG - chr8.4:21599951-21599970 None:intergenic 10.0%
!! ACATGACAAATTATGAATTT+AGG + chr8.4:21598936-21598955 MS.gene011363:CDS 20.0%
!! ATAAGGCTTATCTAATTTAT+TGG - chr8.4:21598891-21598910 None:intergenic 20.0%
!! TATCTAATTTATTGGTATTG+TGG - chr8.4:21598883-21598902 None:intergenic 20.0%
!! TTATTCAACTTAAGCTTATA+AGG - chr8.4:21598908-21598927 None:intergenic 20.0%
! ATAATCAAATACAGACTAAG+TGG - chr8.4:21599250-21599269 None:intergenic 25.0%
! CAAGTGAAAATGTGTTAATT+TGG - chr8.4:21599709-21599728 None:intergenic 25.0%
! TATTCCTAAAATCAAAGCTA+GGG - chr8.4:21598708-21598727 None:intergenic 25.0%
! TCTGTATTTGATTATACGTT+TGG + chr8.4:21599255-21599274 MS.gene011363:intron 25.0%
! TTCAAGCAATTTAACTAACA+TGG - chr8.4:21599204-21599223 None:intergenic 25.0%
!! TTTTCAGATGACTTTTTGAT+TGG - chr8.4:21600182-21600201 None:intergenic 25.0%
!!! GAATTGTTTCTAACTTGTAT+AGG + chr8.4:21599363-21599382 MS.gene011363:intron 25.0%
CTATTCCTAAAATCAAAGCT+AGG - chr8.4:21598709-21598728 None:intergenic 30.0%
CTCAAGATGGAACAAAATAT+TGG + chr8.4:21600045-21600064 MS.gene011363:CDS 30.0%
TAAAATCAAAGCTAGGGATA+AGG - chr8.4:21598702-21598721 None:intergenic 30.0%
TGTAATTAAAAGTGGGATCA+AGG - chr8.4:21599943-21599962 None:intergenic 30.0%
! AACACATTTTCACTTGATGT+AGG + chr8.4:21599713-21599732 MS.gene011363:intron 30.0%
! GTTTTACCTCTGAATAAAAC+TGG - chr8.4:21599863-21599882 None:intergenic 30.0%
! TTTGGAAGTGAACTTTTTGT+TGG - chr8.4:21600206-21600225 None:intergenic 30.0%
!! AGATGACTTTTTGATTGGAT+AGG - chr8.4:21600177-21600196 None:intergenic 30.0%
!! GTTCTGAAGTAGAATTGATT+TGG + chr8.4:21599334-21599353 MS.gene011363:intron 30.0%
!! TAATTACAGGGAGTATTTAC+TGG + chr8.4:21599969-21599988 MS.gene011363:intron 30.0%
!!! TGATTTCCAGTTTTATTCAG+AGG + chr8.4:21599854-21599873 MS.gene011363:CDS 30.0%
!!! TTATCCCTAGCTTTGATTTT+AGG + chr8.4:21598701-21598720 MS.gene011363:CDS 30.0%
AAAGTGAATCAAGGATGCAA+CGG + chr8.4:21599492-21599511 MS.gene011363:CDS 35.0%
AACTTGTATAGGTAGTTGCT+GGG + chr8.4:21599374-21599393 MS.gene011363:intron 35.0%
AATCATCATAGAATGTTCCG+TGG + chr8.4:21598983-21599002 MS.gene011363:CDS 35.0%
AGCATCAGAGAAAAGTTTGT+GGG + chr8.4:21598760-21598779 MS.gene011363:CDS 35.0%
AGGGTTACATTAGAATGCAA+AGG + chr8.4:21600105-21600124 MS.gene011363:CDS 35.0%
ATGTTCCTTCTTCGATTGAT+TGG + chr8.4:21599050-21599069 MS.gene011363:CDS 35.0%
ATTGTGGACATGCATAACAT+TGG - chr8.4:21598867-21598886 None:intergenic 35.0%
ATTTCTGTAGCTATTGATGC+CGG + chr8.4:21599825-21599844 MS.gene011363:CDS 35.0%
GCATCAATAGCTACAGAAAT+CGG - chr8.4:21599825-21599844 None:intergenic 35.0%
GGGAACCTGTAATATACAAA+AGG + chr8.4:21599596-21599615 MS.gene011363:CDS 35.0%
GGGTTACATTAGAATGCAAA+GGG + chr8.4:21600106-21600125 MS.gene011363:CDS 35.0%
GGTTACATTAGAATGCAAAG+GGG + chr8.4:21600107-21600126 MS.gene011363:CDS 35.0%
GTTTGGATCCAAAATTGATC+TGG + chr8.4:21599272-21599291 MS.gene011363:intron 35.0%
TAGCATCAGAGAAAAGTTTG+TGG + chr8.4:21598759-21598778 MS.gene011363:CDS 35.0%
TATCCTTATGCTGCAAAAGA+GGG + chr8.4:21599576-21599595 MS.gene011363:CDS 35.0%
TCAACAATTGTAGCTGTTGA+AGG + chr8.4:21599402-21599421 MS.gene011363:CDS 35.0%
TCCACATTCTCATACATGAA+CGG - chr8.4:21599028-21599047 None:intergenic 35.0%
TTATCCTTATGCTGCAAAAG+AGG + chr8.4:21599575-21599594 MS.gene011363:CDS 35.0%
! GTGCACCTTTTGTATATTAC+AGG - chr8.4:21599604-21599623 None:intergenic 35.0%
! TAACTTGTATAGGTAGTTGC+TGG + chr8.4:21599373-21599392 MS.gene011363:intron 35.0%
! TTCGAGTTCATCAAGCAAAA+TGG + chr8.4:21599534-21599553 MS.gene011363:CDS 35.0%
!! CTAGTTTTCGTGTCTTGATT+TGG - chr8.4:21599434-21599453 None:intergenic 35.0%
AAACCAGCAGTGTCAATTGA+TGG + chr8.4:21599741-21599760 MS.gene011363:CDS 40.0%
AACAAGTAAGCTTACCGCAT+TGG - chr8.4:21599114-21599133 None:intergenic 40.0%
AATCATGGGGTAGCAATTGT+GGG + chr8.4:21600011-21600030 MS.gene011363:CDS 40.0%
ACCGTTCATGTATGAGAATG+TGG + chr8.4:21599024-21599043 MS.gene011363:CDS 40.0%
ATTGGACAGTGAAGAACTCT+TGG + chr8.4:21600063-21600082 MS.gene011363:CDS 40.0%
CAATAGCTACAGAAATCGGT+TGG - chr8.4:21599821-21599840 None:intergenic 40.0%
CATTGTCATGTAACATGCCA+CGG - chr8.4:21599003-21599022 None:intergenic 40.0%
CCAAAATTGATCTGGAGAAC+TGG + chr8.4:21599280-21599299 MS.gene011363:intron 40.0%
GTTCATGTATGAGAATGTGG+AGG + chr8.4:21599027-21599046 MS.gene011363:CDS 40.0%
TCGATTGATTGGAGGAAGAT+AGG + chr8.4:21599061-21599080 MS.gene011363:CDS 40.0%
TGCGACACTAAAGTGAATCA+AGG + chr8.4:21599483-21599502 MS.gene011363:CDS 40.0%
TTCCTCCAATCAATCGAAGA+AGG - chr8.4:21599058-21599077 None:intergenic 40.0%
TTCCTTCTTCGATTGATTGG+AGG + chr8.4:21599053-21599072 MS.gene011363:CDS 40.0%
TTGGACAGTGAAGAACTCTT+GGG + chr8.4:21600064-21600083 MS.gene011363:CDS 40.0%
TTTCTGTAGCTATTGATGCC+GGG + chr8.4:21599826-21599845 MS.gene011363:CDS 40.0%
! AATGCTGCTTCATTGTTAGC+AGG - chr8.4:21599780-21599799 None:intergenic 40.0%
! AGCTAAGAGCTTTGATTTCC+AGG + chr8.4:21598727-21598746 MS.gene011363:CDS 40.0%
! ATGCTGCTTCATTGTTAGCA+GGG - chr8.4:21599779-21599798 None:intergenic 40.0%
! CCAGTTCTCCAGATCAATTT+TGG - chr8.4:21599283-21599302 None:intergenic 40.0%
!! GAGCATCTATGCTGATTCTA+AGG + chr8.4:21598958-21598977 MS.gene011363:CDS 40.0%
AAACTGGAAATCAGATCCCC+CGG - chr8.4:21599847-21599866 None:intergenic 45.0%
AACTCTTGGGGAAGTGAATG+GGG + chr8.4:21600077-21600096 MS.gene011363:CDS 45.0%
AGAACTCTTGGGGAAGTGAA+TGG + chr8.4:21600075-21600094 MS.gene011363:CDS 45.0%
AGCTACAGAAATCGGTTGGT+TGG - chr8.4:21599817-21599836 None:intergenic 45.0%
AGGTCTATGTGGCATAGCTA+TGG + chr8.4:21600145-21600164 MS.gene011363:CDS 45.0%
ATCATGGGGTAGCAATTGTG+GGG + chr8.4:21600012-21600031 MS.gene011363:CDS 45.0%
CAAGACTTCGAGTAACTGTG+TGG - chr8.4:21598811-21598830 None:intergenic 45.0%
CGTGGCATGTTACATGACAA+TGG + chr8.4:21599001-21599020 MS.gene011363:CDS 45.0%
CTGATGCTAAGTCATTCTCC+TGG - chr8.4:21598748-21598767 None:intergenic 45.0%
GAACTCTTGGGGAAGTGAAT+GGG + chr8.4:21600076-21600095 MS.gene011363:CDS 45.0%
GAATCATGGGGTAGCAATTG+TGG + chr8.4:21600010-21600029 MS.gene011363:CDS 45.0%
GCAATCCACAAGTTCTTGCT+CGG - chr8.4:21599466-21599485 None:intergenic 45.0%
GTAAAGGATCAAGGCCAATG+CGG + chr8.4:21599097-21599116 MS.gene011363:CDS 45.0%
TCTATGTGGCATAGCTATGG+AGG + chr8.4:21600148-21600167 MS.gene011363:CDS 45.0%
TCTCACAAGCAAGGTCTATG+TGG + chr8.4:21600134-21600153 MS.gene011363:CDS 45.0%
TGAATCAAGGATGCAACGGT+GGG + chr8.4:21599496-21599515 MS.gene011363:CDS 45.0%
TGGACAGTGAAGAACTCTTG+GGG + chr8.4:21600065-21600084 MS.gene011363:CDS 45.0%
TGTGGGATTTGTACGAGAGA+TGG + chr8.4:21598777-21598796 MS.gene011363:CDS 45.0%
TGTTAGCAGGGACATTCTCA+TGG - chr8.4:21599767-21599786 None:intergenic 45.0%
TTCTGTAGCTATTGATGCCG+GGG + chr8.4:21599827-21599846 MS.gene011363:CDS 45.0%
! GGAGTATTTACTGGACACTG+TGG + chr8.4:21599978-21599997 MS.gene011363:CDS 45.0%
! GTGGTACTGAGCTGAATCAT+GGG + chr8.4:21599997-21600016 MS.gene011363:CDS 45.0%
! GTTCCCTCTTTTGCAGCATA+AGG - chr8.4:21599582-21599601 None:intergenic 45.0%
! TGGTACTGAGCTGAATCATG+GGG + chr8.4:21599998-21600017 MS.gene011363:CDS 45.0%
! TGTGGTACTGAGCTGAATCA+TGG + chr8.4:21599996-21600015 MS.gene011363:CDS 45.0%
CACAAGTTCTTGCTCGGACA+AGG - chr8.4:21599460-21599479 None:intergenic 50.0%
CTTGTCCGAGCAAGAACTTG+TGG + chr8.4:21599458-21599477 MS.gene011363:CDS 50.0%
GGAAGTGAATGGGGAGAACA+GGG + chr8.4:21600086-21600105 MS.gene011363:CDS 50.0%
GGGTATGGAGCAACTCAAGA+TGG + chr8.4:21600032-21600051 MS.gene011363:CDS 50.0%
GTGAATCAAGGATGCAACGG+TGG + chr8.4:21599495-21599514 MS.gene011363:CDS 50.0%
TCTGTAGCTATTGATGCCGG+GGG + chr8.4:21599828-21599847 MS.gene011363:CDS 50.0%
TGGCCATCAATTGACACTGC+TGG - chr8.4:21599747-21599766 None:intergenic 50.0%
! AGGAAGATAGGTGCTGTCAC+CGG + chr8.4:21599073-21599092 MS.gene011363:CDS 50.0%
! AGGGGCATATCTCACAAGCA+AGG + chr8.4:21600125-21600144 MS.gene011363:CDS 50.0%
!! AGGATGCAACGGTGGGTTAA+TGG + chr8.4:21599503-21599522 MS.gene011363:CDS 50.0%
!! TTGGCCTTGATCCTTTACCC+CGG - chr8.4:21599095-21599114 None:intergenic 50.0%
GGGAAGTGAATGGGGAGAAC+AGG + chr8.4:21600085-21600104 MS.gene011363:CDS 55.0%
GGGGTAGCAATTGTGGGGTA+TGG + chr8.4:21600017-21600036 MS.gene011363:CDS 55.0%
GTCACCGGGGTAAAGGATCA+AGG + chr8.4:21599088-21599107 MS.gene011363:CDS 55.0%
! GAAGATAGGTGCTGTCACCG+GGG + chr8.4:21599075-21599094 MS.gene011363:CDS 55.0%
! GGAAGATAGGTGCTGTCACC+GGG + chr8.4:21599074-21599093 MS.gene011363:CDS 55.0%
! AGGTGCTGTCACCGGGGTAA+AGG + chr8.4:21599081-21599100 MS.gene011363:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 21598671 21600238 21598671 ID=MS.gene011363
chr8.4 mRNA 21598671 21600238 21598671 ID=MS.gene011363.t1;Parent=MS.gene011363
chr8.4 exon 21598671 21599118 21598671 ID=MS.gene011363.t1.exon1;Parent=MS.gene011363.t1
chr8.4 CDS 21598671 21599118 21598671 ID=cds.MS.gene011363.t1;Parent=MS.gene011363.t1
chr8.4 exon 21599385 21599617 21599385 ID=MS.gene011363.t1.exon2;Parent=MS.gene011363.t1
chr8.4 CDS 21599385 21599617 21599385 ID=cds.MS.gene011363.t1;Parent=MS.gene011363.t1
chr8.4 exon 21599735 21599875 21599735 ID=MS.gene011363.t1.exon3;Parent=MS.gene011363.t1
chr8.4 CDS 21599735 21599875 21599735 ID=cds.MS.gene011363.t1;Parent=MS.gene011363.t1
chr8.4 exon 21599978 21600238 21599978 ID=MS.gene011363.t1.exon4;Parent=MS.gene011363.t1
chr8.4 CDS 21599978 21600238 21599978 ID=cds.MS.gene011363.t1;Parent=MS.gene011363.t1
Gene Sequence

>MS.gene011363

ATGGAAATGAAGAAGCTCTTGTTTGTTTCCTTATCCCTAGCTTTGATTTTAGGAATAGCTAAGAGCTTTGATTTCCAGGAGAATGACTTAGCATCAGAGAAAAGTTTGTGGGATTTGTACGAGAGATGGAGAAGTCACCACACAGTTACTCGAAGTCTTGATGAGAAGAATAATCGTTTTAATGTGTTCAAAGCCAATGTTATGCATGTCCACAATACCAATAAATTAGATAAGCCTTATAAGCTTAAGTTGAATAAGTTTGCTGACATGACAAATTATGAATTTAGGAGCATCTATGCTGATTCTAAGGTTAATCATCATAGAATGTTCCGTGGCATGTTACATGACAATGGACCGTTCATGTATGAGAATGTGGAGGATGTTCCTTCTTCGATTGATTGGAGGAAGATAGGTGCTGTCACCGGGGTAAAGGATCAAGGCCAATGCGGTAAGCTTACTTGTTGATTAAGTAATCACATACTATTTATAATTTGCGTAAAACATGTTTTTTGATATCTACAAAATACTCACCATGTTAGTTAAATTGCTTGAAGTTTGATAGTTAAGTTTGTTCTTCCACTTAGTCTGTATTTGATTATACGTTTGGATCCAAAATTGATCTGGAGAACTGGAGAAGTGAAATTGATTTTAACATGTTTGATTGTTCTGAAGTAGAATTGATTTGGTCTTTTGAATTGTTTCTAACTTGTATAGGTAGTTGCTGGGCTTTCTCAACAATTGTAGCTGTTGAAGGTATTAACCAAATCAAGACACGAAAACTAGTATCCTTGTCCGAGCAAGAACTTGTGGATTGCGACACTAAAGTGAATCAAGGATGCAACGGTGGGTTAATGGAAAATGCATTCGAGTTCATCAAGCAAAATGGCATAACAACAGAAACTAATTATCCTTATGCTGCAAAAGAGGGAACCTGTAATATACAAAAGGTGCACACACTAGTCTATTTATAATTAGTACTACCTAATATATAATTAATAAATTTTATTTGATTAACTTATGGGAAAATTTAAAGATCCAAATTAACACATTTTCACTTGATGTAGGAGAATAAACCAGCAGTGTCAATTGATGGCCATGAGAATGTCCCTGCTAACAATGAAGCAGCATTGTTGAAAGCTGCTGCCAACCAACCGATTTCTGTAGCTATTGATGCCGGGGGATCTGATTTCCAGTTTTATTCAGAGGTAAAACTTATATAAATTTTTATCACTTACACATTTATATATTTTTGTATAAATTTGTATATCACCTTGATCCCACTTTTAATTACAAAAATATAATTACAGGGAGTATTTACTGGACACTGTGGTACTGAGCTGAATCATGGGGTAGCAATTGTGGGGTATGGAGCAACTCAAGATGGAACAAAATATTGGACAGTGAAGAACTCTTGGGGAAGTGAATGGGGAGAACAGGGTTACATTAGAATGCAAAGGGGCATATCTCACAAGCAAGGTCTATGTGGCATAGCTATGGAGGCTTCCTATCCAATCAAAAAGTCATCTGAAAATCCAACAAAAAGTTCACTTCCAAAAGATGAGCTATAA
Protein sequence

>MS.gene011363.t1

MEMKKLLFVSLSLALILGIAKSFDFQENDLASEKSLWDLYERWRSHHTVTRSLDEKNNRFNVFKANVMHVHNTNKLDKPYKLKLNKFADMTNYEFRSIYADSKVNHHRMFRGMLHDNGPFMYENVEDVPSSIDWRKIGAVTGVKDQGQCGSCWAFSTIVAVEGINQIKTRKLVSLSEQELVDCDTKVNQGCNGGLMENAFEFIKQNGITTETNYPYAAKEGTCNIQKENKPAVSIDGHENVPANNEAALLKAAANQPISVAIDAGGSDFQFYSEGVFTGHCGTELNHGVAIVGYGATQDGTKYWTVKNSWGSEWGEQGYIRMQRGISHKQGLCGIAMEASYPIKKSSENPTKSSLPKDEL