AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003680.t1 MTR_4g071060 97.238 362 10 0 1 362 1 362 0.0 743
MS.gene003680.t1 MTR_1g023210 69.837 368 99 6 1 360 1 364 0.0 529
MS.gene003680.t1 MTR_4g073660 68.132 364 76 6 3 363 4 330 7.98e-177 494
MS.gene003680.t1 MTR_4g072340 67.935 368 76 7 1 363 1 331 1.28e-176 494
MS.gene003680.t1 MTR_4g072790 68.681 364 70 7 3 363 5 327 2.47e-176 493
MS.gene003680.t1 MTR_1g094135 53.352 358 158 5 10 360 15 370 1.50e-140 404
MS.gene003680.t1 MTR_1g107550 42.667 300 146 7 50 341 54 335 5.48e-68 221
MS.gene003680.t1 MTR_4g079770 38.310 355 178 13 5 348 12 336 2.22e-65 211
MS.gene003680.t1 MTR_4g047610 39.748 317 153 10 54 353 44 339 1.27e-64 209
MS.gene003680.t1 MTR_4g079470 39.329 328 168 12 32 348 29 336 1.51e-64 209
MS.gene003680.t1 MTR_3g116080 37.119 361 193 12 4 353 7 344 2.62e-63 206
MS.gene003680.t1 MTR_4g079800 38.750 320 168 11 45 353 39 341 2.98e-63 205
MS.gene003680.t1 MTR_4g125300 37.568 370 189 11 1 353 1 345 3.39e-63 205
MS.gene003680.t1 MTR_1g018840 37.978 366 177 12 1 341 1 341 4.45e-63 209
MS.gene003680.t1 MTR_1g018840 37.978 366 177 12 1 341 1 341 6.56e-63 207
MS.gene003680.t1 MTR_4g080700 37.421 318 174 8 38 348 32 331 8.81e-63 204
MS.gene003680.t1 MTR_5g022560 38.941 321 167 11 45 353 37 340 9.43e-63 204
MS.gene003680.t1 MTR_3g102220 36.467 351 187 11 8 341 10 341 9.85e-63 207
MS.gene003680.t1 MTR_4g094918 41.026 312 135 11 50 341 39 321 2.51e-62 203
MS.gene003680.t1 MTR_4g080730 37.417 302 166 7 54 348 46 331 1.01e-61 201
MS.gene003680.t1 MTR_4g079440 32.865 356 211 9 5 353 7 341 9.36e-61 199
MS.gene003680.t1 MTR_4g107930 38.907 311 164 9 54 353 46 341 3.53e-60 197
MS.gene003680.t1 MTR_3g112430 37.730 326 160 12 48 353 36 338 5.80e-59 197
MS.gene003680.t1 MTR_3g075390 35.574 357 189 11 7 348 6 336 1.43e-58 194
MS.gene003680.t1 MTR_4g080360 35.831 307 169 9 54 348 43 333 9.46e-58 191
MS.gene003680.t1 MTR_2g075830 37.086 302 169 6 54 348 45 332 1.02e-56 188
MS.gene003680.t1 MTR_4g104370 38.361 305 156 10 58 348 49 335 1.09e-56 189
MS.gene003680.t1 MTR_2g015850 39.437 284 149 8 64 341 78 344 1.36e-55 186
MS.gene003680.t1 MTR_4g065077 34.409 372 194 14 5 352 7 352 1.59e-55 186
MS.gene003680.t1 MTR_2g015920 32.796 372 194 14 8 350 8 352 1.13e-54 184
MS.gene003680.t1 MTR_2g090890 35.000 320 180 8 48 359 39 338 2.27e-54 182
MS.gene003680.t1 MTR_2g016180 35.514 321 173 11 48 353 51 352 2.77e-53 183
MS.gene003680.t1 MTR_2g083930 36.336 333 179 14 41 358 30 344 1.59e-52 177
MS.gene003680.t1 MTR_2g016270 35.110 319 162 11 48 353 51 337 1.62e-52 181
MS.gene003680.t1 MTR_8g086470 34.483 348 182 12 5 341 23 335 3.46e-52 177
MS.gene003680.t1 MTR_2g015930 31.129 363 209 12 6 349 11 351 9.88e-49 168
MS.gene003680.t1 MTR_7g078600 32.288 319 179 9 42 348 36 329 1.65e-46 162
MS.gene003680.t1 MTR_2g067900 33.333 333 181 13 42 353 10 322 2.02e-44 156
MS.gene003680.t1 MTR_7g079230 31.661 319 182 9 42 348 33 327 8.33e-43 152
MS.gene003680.t1 MTR_8g087510 50.000 136 57 3 172 306 23 148 6.33e-41 142
MS.gene003680.t1 MTR_1g023810 40.991 222 111 7 129 347 47 251 1.61e-40 144
MS.gene003680.t1 MTR_2g064475 31.165 369 176 15 44 362 12 352 1.02e-39 144
MS.gene003680.t1 MTR_2g067560 33.639 327 164 13 42 341 10 310 7.37e-38 144
MS.gene003680.t1 MTR_2g066980 28.158 380 183 16 1 355 1 315 1.47e-32 124
MS.gene003680.t1 MTR_7g111050 30.000 290 152 14 95 353 63 332 1.58e-23 100
MS.gene003680.t1 MTR_7g078630 27.206 272 154 5 48 308 706 944 1.71e-23 102
MS.gene003680.t1 MTR_7g111060 29.268 287 158 12 95 353 66 335 1.48e-22 97.8
MS.gene003680.t1 MTR_1g025450 38.043 184 85 7 126 306 50 207 7.72e-22 97.1
MS.gene003680.t1 MTR_2g015840 36.879 141 79 3 54 184 39 179 2.67e-19 87.0
MS.gene003680.t1 MTR_2g015840 34.746 118 69 3 244 360 161 271 1.16e-13 70.9
MS.gene003680.t1 MTR_2g047960 42.391 92 45 1 135 226 2 85 4.56e-15 70.9
MS.gene003680.t1 MTR_7g078310 41.584 101 50 2 130 228 151 244 4.62e-15 74.7
MS.gene003680.t1 MTR_3g029560 27.737 274 139 14 104 358 4 237 1.12e-12 67.4
MS.gene003680.t1 MTR_7g111050 29.098 244 128 13 95 307 58 287 1.03e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003680.t1 AT2G21430 73.611 360 92 3 1 358 1 359 0.0 558
MS.gene003680.t1 AT4G39090 73.109 357 94 2 8 362 10 366 0.0 552
MS.gene003680.t1 AT4G16190 67.292 373 112 4 1 363 1 373 0.0 509
MS.gene003680.t1 AT3G54940 58.172 361 134 7 6 356 9 362 1.38e-152 434
MS.gene003680.t1 AT3G54940 53.455 275 112 6 6 271 9 276 7.13e-99 295
MS.gene003680.t1 AT3G19400 40.968 310 153 9 57 353 53 345 5.70e-67 216
MS.gene003680.t1 AT3G45310 38.483 356 184 11 10 348 11 348 1.93e-66 214
MS.gene003680.t1 AT5G60360 39.039 333 175 9 31 353 39 353 2.81e-66 214
MS.gene003680.t1 AT3G19390 38.504 361 181 12 8 353 8 342 1.84e-65 214
MS.gene003680.t1 AT5G60360 39.450 327 170 9 31 347 39 347 3.51e-65 211
MS.gene003680.t1 AT3G45310 38.136 354 184 11 10 346 11 346 4.73e-65 210
MS.gene003680.t1 AT5G60360 39.264 326 170 9 31 346 39 346 8.34e-65 210
MS.gene003680.t1 AT1G20850 39.117 317 165 8 48 353 51 350 2.11e-64 209
MS.gene003680.t1 AT1G09850 39.198 324 166 11 48 359 32 336 3.63e-64 211
MS.gene003680.t1 AT1G47128 37.971 345 183 8 9 341 8 333 2.30e-63 209
MS.gene003680.t1 AT5G43060 42.199 282 142 7 64 341 70 334 5.52e-63 208
MS.gene003680.t1 AT1G29090 39.564 321 160 12 44 348 44 346 1.44e-62 204
MS.gene003680.t1 AT5G45890 34.153 366 192 10 1 348 3 337 5.85e-62 202
MS.gene003680.t1 AT4G35350 37.025 316 172 8 48 353 51 349 3.17e-61 201
MS.gene003680.t1 AT1G06260 37.846 325 161 13 45 353 40 339 5.27e-61 199
MS.gene003680.t1 AT3G43960 40.614 293 153 7 56 341 49 327 8.95e-61 200
MS.gene003680.t1 AT4G36880 38.754 289 150 6 64 341 69 341 4.25e-59 196
MS.gene003680.t1 AT3G48340 36.494 348 179 11 9 341 4 324 4.01e-58 192
MS.gene003680.t1 AT2G27420 36.278 317 162 11 54 348 41 339 2.35e-57 190
MS.gene003680.t1 AT4G23520 33.424 368 195 14 5 353 10 346 2.53e-57 190
MS.gene003680.t1 AT5G50260 39.744 312 143 11 58 348 47 334 9.90e-57 189
MS.gene003680.t1 AT1G29080 36.943 314 168 9 54 353 45 342 1.14e-54 183
MS.gene003680.t1 AT3G48350 34.857 350 187 11 7 342 3 325 1.27e-54 184
MS.gene003680.t1 AT4G11310 32.591 359 203 10 6 348 9 344 1.28e-52 178
MS.gene003680.t1 AT2G34080 35.691 311 165 11 54 348 45 336 4.00e-52 176
MS.gene003680.t1 AT4G11320 34.921 315 172 8 48 348 56 351 1.06e-51 176
MS.gene003680.t1 AT4G11320 34.921 315 172 8 48 348 56 351 1.06e-51 176
MS.gene003680.t1 AT3G49340 33.866 313 174 11 54 351 41 335 2.17e-51 174
MS.gene003680.t1 AT3G19400 40.889 225 121 4 57 276 53 270 1.48e-50 171
MS.gene003680.t1 AT4G35350 36.052 233 136 4 48 276 51 274 9.24e-46 158
MS.gene003680.t1 AT1G29110 32.154 311 166 9 54 348 44 325 2.26e-42 150
MS.gene003680.t1 AT3G48350 32.787 305 155 10 7 287 3 281 9.20e-39 140
MS.gene003680.t1 AT4G01610 29.874 318 168 14 61 349 40 331 1.51e-23 100
MS.gene003680.t1 AT1G02305 30.556 288 151 15 95 352 69 337 1.31e-22 97.8
MS.gene003680.t1 AT4G01610 29.560 318 169 14 61 349 40 331 2.31e-22 97.1
MS.gene003680.t1 AT1G02300 27.508 309 153 16 95 352 66 354 3.95e-18 85.1

Find 87 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATATATACTGCATTTA+AGG 0.130654 4.1:+31979855 None:intergenic
AACCTATTTAAACATAAGTT+TGG 0.234066 4.1:-31981195 MS.gene003680:CDS
TCTCTCCAATCAAAATCTTT+TGG 0.234300 4.1:+31980946 None:intergenic
TCCCAAACTTATGTTTAAAT+AGG 0.268902 4.1:+31981193 None:intergenic
CCAAGGATGAACATGATTAC+AGG 0.304516 4.1:-31981158 MS.gene003680:CDS
GGTGAGACTTGGGGAGAAAA+TGG 0.310928 4.1:-31979691 MS.gene003680:CDS
CTGGAGCCTATTCGCCTATT+AGG 0.320950 4.1:-31979753 MS.gene003680:CDS
GGAGTTTCGTCAGAGCGTTT+TGG 0.321592 4.1:-31981034 MS.gene003680:CDS
CCACGATCAGTAGCAGAATA+AGG 0.357295 4.1:+31980098 None:intergenic
ACTAGATCATGGAGTGTTAC+TGG 0.371273 4.1:-31979788 MS.gene003680:CDS
ACCTATTTAAACATAAGTTT+GGG 0.393244 4.1:-31981194 MS.gene003680:CDS
AGTCTCTTTGAGCATATGTA+TGG 0.401014 4.1:+31979808 None:intergenic
AGATTATCTGTGGGAAGAAT+CGG 0.407382 4.1:+31980970 None:intergenic
GGCTTCTCCTTCATCCTAAT+AGG 0.407412 4.1:+31979739 None:intergenic
TCACAAGAACCCGGTTGCTC+TGG 0.408096 4.1:+31980203 None:intergenic
GGAGTGTTACTGGTGGGATA+TGG 0.410428 4.1:-31979778 MS.gene003680:CDS
ATTGAATCTTCTCCGTCATC+CGG 0.411609 4.1:+31981273 None:intergenic
GGGGCTGCATTAGCATCTTT+CGG 0.417402 4.1:+31980991 None:intergenic
GGATGAAGGAGAAGCCATAT+TGG 0.423435 4.1:-31979732 MS.gene003680:CDS
TGCTAAGAAGCATCAATTGA+TGG 0.425569 4.1:-31981106 MS.gene003680:CDS
GATTATCTGTGGGAAGAATC+GGG 0.426123 4.1:+31980971 None:intergenic
CTTCATCCTAATAGGCGAAT+AGG 0.432818 4.1:+31979747 None:intergenic
TGACAAGATTAGCGGCAATC+TGG 0.437809 4.1:+31980003 None:intergenic
CAACCGGGTTCTTGTGATGC+CGG 0.438520 4.1:-31980197 MS.gene003680:CDS
ATAGTGTGATCCAGAGCAAC+CGG 0.450276 4.1:-31980213 MS.gene003680:intron
TCTTGTGATGCCGGGTGCAA+TGG 0.456716 4.1:-31980188 MS.gene003680:CDS
CCTGTAATCATGTTCATCCT+TGG 0.456777 4.1:+31981158 None:intergenic
AAAACTCATGGGGTGAGACT+TGG 0.466256 4.1:-31979702 MS.gene003680:CDS
GCATCAATTGATGGATCCTT+CGG 0.466749 4.1:-31981097 MS.gene003680:CDS
TGTTGGAGTTTCAGTACAAC+CGG 0.469204 4.1:-31980767 MS.gene003680:CDS
ATGGATCCTTCGGCGGTTCA+CGG 0.470073 4.1:-31981087 MS.gene003680:CDS
AAACTCATGGGGTGAGACTT+GGG 0.470356 4.1:-31979701 MS.gene003680:CDS
CGACAGGTGGTCGACGATGA+AGG 0.470944 4.1:-31981246 MS.gene003680:CDS
ATGGATACTACAAAATATGC+AGG 0.476628 4.1:-31979672 MS.gene003680:CDS
GACGATGAAGGAGTGCGGTT+GGG 0.489192 4.1:-31981234 MS.gene003680:CDS
AACCGGGTTCTTGTGATGCC+GGG 0.494687 4.1:-31980196 MS.gene003680:CDS
TTACTGGTGGGATATGGATC+TGG 0.507620 4.1:-31979772 MS.gene003680:CDS
TCAATTGATGCTTCTTAGCA+CGG 0.511562 4.1:+31981109 None:intergenic
TGGGAAGGTTTATTCTTCCA+AGG 0.519917 4.1:-31981175 MS.gene003680:CDS
GGTGCTCACTTTCTCTCGAC+CGG 0.523916 4.1:-31980734 MS.gene003680:CDS
CGACGATGAAGGAGTGCGGT+TGG 0.531274 4.1:-31981235 MS.gene003680:CDS
CCTTATTCTGCTACTGATCG+TGG 0.532697 4.1:-31980098 MS.gene003680:CDS
AGGGCTCGTGTGGGTCATGT+TGG 0.536407 4.1:-31980784 MS.gene003680:CDS
CTTTAGCACTTACTAGCAAG+AGG 0.547021 4.1:+31979972 None:intergenic
ACCGTTCTTGACAAGATTAG+CGG 0.547122 4.1:+31979995 None:intergenic
AGTACAACCGGTGCACTTGA+AGG 0.552820 4.1:-31980755 MS.gene003680:CDS
AAACTCCCTCGGAGTAAGAT+CGG 0.553683 4.1:+31981050 None:intergenic
TTCTTCGCGCATCACCCCAC+CGG 0.555594 4.1:+31980127 None:intergenic
CATCAGACCACCATTGCACC+CGG 0.562811 4.1:+31980178 None:intergenic
CTTCATCGTCGACCACCTGT+CGG 0.563999 4.1:+31981247 None:intergenic
CTATTCGCCTATTAGGATGA+AGG 0.564118 4.1:-31979746 MS.gene003680:CDS
TGCTCAAAGAGACTAGATCA+TGG 0.566764 4.1:-31979799 MS.gene003680:CDS
AGAAGATTCAATAATCCGAC+AGG 0.575409 4.1:-31981262 MS.gene003680:CDS
TGCAGGGGACGAAATATATG+TGG 0.578645 4.1:-31979655 MS.gene003680:CDS
GTCTCTTTGAGCATATGTAT+GGG 0.587803 4.1:+31979809 None:intergenic
ATTTAAACATAAGTTTGGGA+AGG 0.587990 4.1:-31981190 MS.gene003680:CDS
GCCGCTAATCTTGTCAAGAA+CGG 0.589186 4.1:-31979996 MS.gene003680:CDS
GTGAGCACCTTCAAGTGCAC+CGG 0.592969 4.1:+31980748 None:intergenic
AACTCATGGGGTGAGACTTG+GGG 0.593059 4.1:-31979700 MS.gene003680:CDS
ACTTGTCGATTGTGATCATG+AGG 0.595475 4.1:-31980684 MS.gene003680:intron
TATATGTGGAGTGGACTCCA+TGG 0.597639 4.1:-31979641 MS.gene003680:CDS
TGGATACTACAAAATATGCA+GGG 0.599253 4.1:-31979671 MS.gene003680:CDS
GTATATATGCAAACATACAT+TGG 0.601664 4.1:-31979844 MS.gene003680:CDS
TGTGATGCCGGGTGCAATGG+TGG 0.601772 4.1:-31980185 MS.gene003680:CDS
ACGCTCTGACGAAACTCCCT+CGG 0.605558 4.1:+31981039 None:intergenic
ATTCTCCGATCTTACTCCGA+GGG 0.607597 4.1:-31981055 MS.gene003680:CDS
CACCCGGCATCACAAGAACC+CGG 0.609271 4.1:+31980194 None:intergenic
TATATGCAAACATACATTGG+AGG 0.612222 4.1:-31979841 MS.gene003680:CDS
GCATTCTCTACGCCGGATGA+CGG 0.614941 4.1:-31981285 MS.gene003680:CDS
GGACTCCATGGTTTCTACTG+TGG 0.626354 4.1:-31979629 MS.gene003680:CDS
CGGCGTAGAGAATGCCGAGA+CGG 0.629708 4.1:+31981293 None:intergenic
TCAATTGATGGATCCTTCGG+CGG 0.633662 4.1:-31981094 MS.gene003680:CDS
GATCATGGAGTGTTACTGGT+GGG 0.636411 4.1:-31979784 MS.gene003680:CDS
GGGACGAAATATATGTGGAG+TGG 0.637649 4.1:-31979650 MS.gene003680:CDS
GTAACACCGTGAACCGCCGA+AGG 0.638844 4.1:+31981081 None:intergenic
TAGTGTGATCCAGAGCAACC+GGG 0.639888 4.1:-31980212 MS.gene003680:CDS
TTACTGAGTAGTAGTATGAA+CGG 0.642920 4.1:+31979603 None:intergenic
CGTCTCGGCATTCTCTACGC+CGG 0.649156 4.1:-31981292 MS.gene003680:CDS
TAATGATTCAGGGCTCGTGT+GGG 0.650844 4.1:-31980793 MS.gene003680:intron
GGCAGCCACAGTAGAAACCA+TGG 0.655273 4.1:+31979624 None:intergenic
ATAATGATTCAGGGCTCGTG+TGG 0.664587 4.1:-31980794 MS.gene003680:intron
AGATCATGGAGTGTTACTGG+TGG 0.667806 4.1:-31979785 MS.gene003680:CDS
GGATACTACAAAATATGCAG+GGG 0.670950 4.1:-31979670 MS.gene003680:CDS
AGATTCAATAATCCGACAGG+TGG 0.672731 4.1:-31981259 MS.gene003680:CDS
ATTATCTGTGGGAAGAATCG+GGG 0.677726 4.1:+31980972 None:intergenic
AATTCTCCGATCTTACTCCG+AGG 0.702571 4.1:-31981056 MS.gene003680:CDS
TGGTCGACGATGAAGGAGTG+CGG 0.713653 4.1:-31981239 MS.gene003680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTTTACAAAATTATTTAT+TGG - chr4.1:31980616-31980635 MS.gene003680:intron 10.0%
!! TCTTTACAAAATTATTTATT+GGG - chr4.1:31980617-31980636 MS.gene003680:intron 10.0%
!! TTATTATATGTTATCTAAAT+TGG - chr4.1:31980682-31980701 MS.gene003680:intron 10.0%
!! AGATATACTATTAATTACAA+AGG + chr4.1:31980307-31980326 None:intergenic 15.0%
!! ATAATAATCATAATCACAAT+TGG + chr4.1:31980062-31980081 None:intergenic 15.0%
!! GATATACTATTAATTACAAA+GGG + chr4.1:31980306-31980325 None:intergenic 15.0%
!! AACCTATTTAAACATAAGTT+TGG - chr4.1:31979747-31979766 MS.gene003680:CDS 20.0%
!! AATTAATAGTATATCTGCTA+AGG - chr4.1:31980311-31980330 MS.gene003680:intron 20.0%
!! ACCTATTTAAACATAAGTTT+GGG - chr4.1:31979748-31979767 MS.gene003680:CDS 20.0%
!! ATACAATATCTAATAGAGTT+TGG + chr4.1:31980451-31980470 None:intergenic 20.0%
!! ATATGTTATCTAAATTGGTT+TGG - chr4.1:31980687-31980706 MS.gene003680:CDS 20.0%
!! ATTAATTACAAAGGGAAAAT+AGG + chr4.1:31980298-31980317 None:intergenic 20.0%
!! ATTGGATAATTAAAAACTCA+TGG - chr4.1:31981228-31981247 MS.gene003680:CDS 20.0%
!! TATCTGATATTAATCTTTCT+AGG - chr4.1:31980395-31980414 MS.gene003680:intron 20.0%
!! TTGGATAATTAAAAACTCAT+GGG - chr4.1:31981229-31981248 MS.gene003680:CDS 20.0%
!!! AAAACAAATTTTGATTGTGT+TGG - chr4.1:31980118-31980137 MS.gene003680:CDS 20.0%
!!! AAACAAATTTTGATTGTGTT+GGG - chr4.1:31980119-31980138 MS.gene003680:CDS 20.0%
!!! AAATGCTAACTTGTAATATT+TGG - chr4.1:31980640-31980659 MS.gene003680:intron 20.0%
!!! AATGCTAACTTGTAATATTT+GGG - chr4.1:31980641-31980660 MS.gene003680:intron 20.0%
!!! GAGTTTTTAATTATCCAATA+TGG + chr4.1:31981227-31981246 None:intergenic 20.0%
!!! TTTGAGTACATTTTAAAATC+CGG - chr4.1:31980796-31980815 MS.gene003680:intron 20.0%
! ACTCTATTAGATATTGTATG+AGG - chr4.1:31980452-31980471 MS.gene003680:intron 25.0%
! ATTTAAACATAAGTTTGGGA+AGG - chr4.1:31979752-31979771 MS.gene003680:CDS 25.0%
! GTATATATGCAAACATACAT+TGG - chr4.1:31981098-31981117 MS.gene003680:CDS 25.0%
! TCCCAAACTTATGTTTAAAT+AGG + chr4.1:31979752-31979771 None:intergenic 25.0%
! TGGATAATTAAAAACTCATG+GGG - chr4.1:31981230-31981249 MS.gene003680:CDS 25.0%
! TTGCATATATACTGCATTTA+AGG + chr4.1:31981090-31981109 None:intergenic 25.0%
!! AGCAAATTTGAAACATGATT+AGG - chr4.1:31980548-31980567 MS.gene003680:intron 25.0%
!! ATGCTAACTTGTAATATTTG+GGG - chr4.1:31980642-31980661 MS.gene003680:intron 25.0%
!!! ATCTTCCAAAAGATTTTGAT+TGG - chr4.1:31979991-31980010 MS.gene003680:CDS 25.0%
!!! TTATCTAAATTGGTTTGGAT+TGG - chr4.1:31980692-31980711 MS.gene003680:CDS 25.0%
AGTTACTGCTGTTAAAAATC+AGG - chr4.1:31980028-31980047 MS.gene003680:CDS 30.0%
ATGGATACTACAAAATATGC+AGG - chr4.1:31981270-31981289 MS.gene003680:CDS 30.0%
TATATGCAAACATACATTGG+AGG - chr4.1:31981101-31981120 MS.gene003680:CDS 30.0%
TGGATACTACAAAATATGCA+GGG - chr4.1:31981271-31981290 MS.gene003680:CDS 30.0%
TTACTGAGTAGTAGTATGAA+CGG + chr4.1:31981342-31981361 None:intergenic 30.0%
! TCTCTCCAATCAAAATCTTT+TGG + chr4.1:31979999-31980018 None:intergenic 30.0%
!! ATCTTTTGGAAGATTATCTG+TGG + chr4.1:31979985-31980004 None:intergenic 30.0%
!! TCTTTTGGAAGATTATCTGT+GGG + chr4.1:31979984-31980003 None:intergenic 30.0%
!!! AGTACATTTTAAAATCCGGT+GGG - chr4.1:31980800-31980819 MS.gene003680:intron 30.0%
AGAAGATTCAATAATCCGAC+AGG - chr4.1:31979680-31979699 MS.gene003680:CDS 35.0%
AGATTATCTGTGGGAAGAAT+CGG + chr4.1:31979975-31979994 None:intergenic 35.0%
AGTCTCTTTGAGCATATGTA+TGG + chr4.1:31981137-31981156 None:intergenic 35.0%
ATTGTATGAGGTTCGACTTA+AGG - chr4.1:31980464-31980483 MS.gene003680:intron 35.0%
GGATACTACAAAATATGCAG+GGG - chr4.1:31981272-31981291 MS.gene003680:CDS 35.0%
GTCTCTTTGAGCATATGTAT+GGG + chr4.1:31981136-31981155 None:intergenic 35.0%
TCAATTGATGCTTCTTAGCA+CGG + chr4.1:31979836-31979855 None:intergenic 35.0%
! GGGTTTGTGATAATGATTCA+GGG - chr4.1:31980139-31980158 MS.gene003680:CDS 35.0%
! TGGGTTTGTGATAATGATTC+AGG - chr4.1:31980138-31980157 MS.gene003680:CDS 35.0%
!! TGCTAAGAAGCATCAATTGA+TGG - chr4.1:31979836-31979855 MS.gene003680:CDS 35.0%
!!! GAGTACATTTTAAAATCCGG+TGG - chr4.1:31980799-31980818 MS.gene003680:intron 35.0%
!!! GATTTTGATTGGAGAGAGAA+AGG - chr4.1:31980002-31980021 MS.gene003680:CDS 35.0%
!!! GTACATTTTAAAATCCGGTG+GGG - chr4.1:31980801-31980820 MS.gene003680:intron 35.0%
ACCGTTCTTGACAAGATTAG+CGG + chr4.1:31980950-31980969 None:intergenic 40.0%
AGATTCAATAATCCGACAGG+TGG - chr4.1:31979683-31979702 MS.gene003680:CDS 40.0%
ATTATCTGTGGGAAGAATCG+GGG + chr4.1:31979973-31979992 None:intergenic 40.0%
ATTGAATCTTCTCCGTCATC+CGG + chr4.1:31979672-31979691 None:intergenic 40.0%
CCAAGGATGAACATGATTAC+AGG - chr4.1:31979784-31979803 MS.gene003680:CDS 40.0%
CCTGTAATCATGTTCATCCT+TGG + chr4.1:31979787-31979806 None:intergenic 40.0%
CTATTCGCCTATTAGGATGA+AGG - chr4.1:31981196-31981215 MS.gene003680:CDS 40.0%
CTTCATCCTAATAGGCGAAT+AGG + chr4.1:31981198-31981217 None:intergenic 40.0%
CTTTAGCACTTACTAGCAAG+AGG + chr4.1:31980973-31980992 None:intergenic 40.0%
GATTATCTGTGGGAAGAATC+GGG + chr4.1:31979974-31979993 None:intergenic 40.0%
TGCTCAAAGAGACTAGATCA+TGG - chr4.1:31981143-31981162 MS.gene003680:CDS 40.0%
TGGGAAGGTTTATTCTTCCA+AGG - chr4.1:31979767-31979786 MS.gene003680:CDS 40.0%
TGTTGGAGTTTCAGTACAAC+CGG - chr4.1:31980175-31980194 MS.gene003680:CDS 40.0%
! ACTAGATCATGGAGTGTTAC+TGG - chr4.1:31981154-31981173 MS.gene003680:CDS 40.0%
! ACTTGTCGATTGTGATCATG+AGG - chr4.1:31980258-31980277 MS.gene003680:intron 40.0%
! GCATCAATTGATGGATCCTT+CGG - chr4.1:31979845-31979864 MS.gene003680:CDS 40.0%
! GCTAAGACTGACAAGTTTTC+CGG + chr4.1:31980230-31980249 None:intergenic 40.0%
! TCTTTTCATCTTCTCCGTCT+CGG - chr4.1:31979635-31979654 MS.gene003680:CDS 40.0%
AAAACTCATGGGGTGAGACT+TGG - chr4.1:31981240-31981259 MS.gene003680:CDS 45.0%
AAACTCATGGGGTGAGACTT+GGG - chr4.1:31981241-31981260 MS.gene003680:CDS 45.0%
AAACTCCCTCGGAGTAAGAT+CGG + chr4.1:31979895-31979914 None:intergenic 45.0%
AATTCTCCGATCTTACTCCG+AGG - chr4.1:31979886-31979905 MS.gene003680:intron 45.0%
ATAATGATTCAGGGCTCGTG+TGG - chr4.1:31980148-31980167 MS.gene003680:CDS 45.0%
ATAGTGTGATCCAGAGCAAC+CGG - chr4.1:31980729-31980748 MS.gene003680:CDS 45.0%
ATTCTCCGATCTTACTCCGA+GGG - chr4.1:31979887-31979906 MS.gene003680:intron 45.0%
CCACGATCAGTAGCAGAATA+AGG + chr4.1:31980847-31980866 None:intergenic 45.0%
CCTTATTCTGCTACTGATCG+TGG - chr4.1:31980844-31980863 MS.gene003680:intron 45.0%
GCCGCTAATCTTGTCAAGAA+CGG - chr4.1:31980946-31980965 MS.gene003680:CDS 45.0%
GGATGAAGGAGAAGCCATAT+TGG - chr4.1:31981210-31981229 MS.gene003680:CDS 45.0%
GGCTTCTCCTTCATCCTAAT+AGG + chr4.1:31981206-31981225 None:intergenic 45.0%
GGGACGAAATATATGTGGAG+TGG - chr4.1:31981292-31981311 MS.gene003680:CDS 45.0%
TAATGATTCAGGGCTCGTGT+GGG - chr4.1:31980149-31980168 MS.gene003680:CDS 45.0%
TGACAAGATTAGCGGCAATC+TGG + chr4.1:31980942-31980961 None:intergenic 45.0%
TGCAGGGGACGAAATATATG+TGG - chr4.1:31981287-31981306 MS.gene003680:CDS 45.0%
TTACTGGTGGGATATGGATC+TGG - chr4.1:31981170-31981189 MS.gene003680:CDS 45.0%
! AGATCATGGAGTGTTACTGG+TGG - chr4.1:31981157-31981176 MS.gene003680:CDS 45.0%
! GATCATGGAGTGTTACTGGT+GGG - chr4.1:31981158-31981177 MS.gene003680:CDS 45.0%
! GCACATCGATAGTGACTTGT+AGG - chr4.1:31980500-31980519 MS.gene003680:intron 45.0%
! TATATGTGGAGTGGACTCCA+TGG - chr4.1:31981301-31981320 MS.gene003680:CDS 45.0%
! TCAATTGATGGATCCTTCGG+CGG - chr4.1:31979848-31979867 MS.gene003680:CDS 45.0%
!! GAGTTTCGTCAGAGCGTTTT+GGG - chr4.1:31979909-31979928 MS.gene003680:intron 45.0%
!!! AGAGCGTTTTGGGATTGAGA+GGG - chr4.1:31979919-31979938 MS.gene003680:intron 45.0%
AACTCATGGGGTGAGACTTG+GGG - chr4.1:31981242-31981261 MS.gene003680:CDS 50.0%
AGTACAACCGGTGCACTTGA+AGG - chr4.1:31980187-31980206 MS.gene003680:CDS 50.0%
CTGGAGCCTATTCGCCTATT+AGG - chr4.1:31981189-31981208 MS.gene003680:CDS 50.0%
GGACTCCATGGTTTCTACTG+TGG - chr4.1:31981313-31981332 MS.gene003680:CDS 50.0%
GGGGCTGCATTAGCATCTTT+CGG + chr4.1:31979954-31979973 None:intergenic 50.0%
GGTGAGACTTGGGGAGAAAA+TGG - chr4.1:31981251-31981270 MS.gene003680:CDS 50.0%
TAGTGTGATCCAGAGCAACC+GGG - chr4.1:31980730-31980749 MS.gene003680:CDS 50.0%
! GGAGTGTTACTGGTGGGATA+TGG - chr4.1:31981164-31981183 MS.gene003680:CDS 50.0%
!! GGAGTTTCGTCAGAGCGTTT+TGG - chr4.1:31979908-31979927 MS.gene003680:intron 50.0%
!!! CAGAGCGTTTTGGGATTGAG+AGG - chr4.1:31979918-31979937 MS.gene003680:intron 50.0%
!!! GAGCGTTTTGGGATTGAGAG+GGG - chr4.1:31979920-31979939 MS.gene003680:intron 50.0%
!!! GTTTTGGGATTGAGAGGGGT+GGG - chr4.1:31979924-31979943 MS.gene003680:intron 50.0%
!!! TTTTGGGATTGAGAGGGGTG+GGG - chr4.1:31979925-31979944 MS.gene003680:intron 50.0%
AACCGGGTTCTTGTGATGCC+GGG - chr4.1:31980746-31980765 MS.gene003680:CDS 55.0%
ACGCTCTGACGAAACTCCCT+CGG + chr4.1:31979906-31979925 None:intergenic 55.0%
ATGGATCCTTCGGCGGTTCA+CGG - chr4.1:31979855-31979874 MS.gene003680:CDS 55.0%
CAACCGGGTTCTTGTGATGC+CGG - chr4.1:31980745-31980764 MS.gene003680:CDS 55.0%
CATCAGACCACCATTGCACC+CGG + chr4.1:31980767-31980786 None:intergenic 55.0%
CTTCATCGTCGACCACCTGT+CGG + chr4.1:31979698-31979717 None:intergenic 55.0%
GCATTCTCTACGCCGGATGA+CGG - chr4.1:31979657-31979676 MS.gene003680:CDS 55.0%
GGCAGCCACAGTAGAAACCA+TGG + chr4.1:31981321-31981340 None:intergenic 55.0%
GGTGCTCACTTTCTCTCGAC+CGG - chr4.1:31980208-31980227 MS.gene003680:CDS 55.0%
GTGAGCACCTTCAAGTGCAC+CGG + chr4.1:31980197-31980216 None:intergenic 55.0%
TCACAAGAACCCGGTTGCTC+TGG + chr4.1:31980742-31980761 None:intergenic 55.0%
TCTTGTGATGCCGGGTGCAA+TGG - chr4.1:31980754-31980773 MS.gene003680:CDS 55.0%
TGGTCGACGATGAAGGAGTG+CGG - chr4.1:31979703-31979722 MS.gene003680:CDS 55.0%
! GACGATGAAGGAGTGCGGTT+GGG - chr4.1:31979708-31979727 MS.gene003680:CDS 55.0%
!!! CGTTTTGGGATTGAGAGGGG+TGG - chr4.1:31979923-31979942 MS.gene003680:intron 55.0%
AGGGCTCGTGTGGGTCATGT+TGG - chr4.1:31980158-31980177 MS.gene003680:CDS 60.0%
CACCCGGCATCACAAGAACC+CGG + chr4.1:31980751-31980770 None:intergenic 60.0%
CGACAGGTGGTCGACGATGA+AGG - chr4.1:31979696-31979715 MS.gene003680:CDS 60.0%
CGGCGTAGAGAATGCCGAGA+CGG + chr4.1:31979652-31979671 None:intergenic 60.0%
CGTCTCGGCATTCTCTACGC+CGG - chr4.1:31979650-31979669 MS.gene003680:CDS 60.0%
GTAACACCGTGAACCGCCGA+AGG + chr4.1:31979864-31979883 None:intergenic 60.0%
TGTGATGCCGGGTGCAATGG+TGG - chr4.1:31980757-31980776 MS.gene003680:CDS 60.0%
TTCTTCGCGCATCACCCCAC+CGG + chr4.1:31980818-31980837 None:intergenic 60.0%
! CGACGATGAAGGAGTGCGGT+TGG - chr4.1:31979707-31979726 MS.gene003680:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 31979603 31981361 31979603 ID=MS.gene003680
chr4.1 mRNA 31979603 31981361 31979603 ID=MS.gene003680.t1;Parent=MS.gene003680
chr4.1 exon 31980915 31981361 31980915 ID=MS.gene003680.t1.exon1;Parent=MS.gene003680.t1
chr4.1 CDS 31980915 31981361 31980915 ID=cds.MS.gene003680.t1;Parent=MS.gene003680.t1
chr4.1 exon 31980685 31980804 31980685 ID=MS.gene003680.t1.exon2;Parent=MS.gene003680.t1
chr4.1 CDS 31980685 31980804 31980685 ID=cds.MS.gene003680.t1;Parent=MS.gene003680.t1
chr4.1 exon 31979985 31980231 31979985 ID=MS.gene003680.t1.exon3;Parent=MS.gene003680.t1
chr4.1 CDS 31979985 31980231 31979985 ID=cds.MS.gene003680.t1;Parent=MS.gene003680.t1
chr4.1 exon 31979603 31979880 31979603 ID=MS.gene003680.t1.exon4;Parent=MS.gene003680.t1
chr4.1 CDS 31979603 31979880 31979603 ID=cds.MS.gene003680.t1;Parent=MS.gene003680.t1
Gene Sequence

>MS.gene003680

ATGGATCATCGCACTCTTCTTCTCTTCGTCGTTCTTTTCATCTTCTCCGTCTCGGCATTCTCTACGCCGGATGACGGAGAAGATTCAATAATCCGACAGGTGGTCGACGATGAAGGAGTGCGGTTGGGAGCAGAGCATCACTTTAACCTATTTAAACATAAGTTTGGGAAGGTTTATTCTTCCAAGGATGAACATGATTACAGGTTTAAAATCTTCAAGTCTAATCTGAACCGTGCTAAGAAGCATCAATTGATGGATCCTTCGGCGGTTCACGGTGTTACGAAATTCTCCGATCTTACTCCGAGGGAGTTTCGTCAGAGCGTTTTGGGATTGAGAGGGGTGGGGTTGCCGAAAGATGCTAATGCAGCCCCGATTCTTCCCACAGATAATCTTCCAAAAGATTTTGATTGGAGAGAGAAAGGAGCAGTTACTGCTGTTAAAAATCAGGTATTGTTTCCAATTGTGATTATGATTATTATTTGTTTATTATTATTGTTTCGTTTAAAAAAAAAAAAAAAACAAATTTTGATTGTGTTGGGTTTGTGATAATGATTCAGGGCTCGTGTGGGTCATGTTGGAGTTTCAGTACAACCGGTGCACTTGAAGGTGCTCACTTTCTCTCGACCGGAAAACTTGTCAGTCTTAGCGAGCAACAACTTGTCGATTGTGATCATGAGGTTTTTTCTTCTTTTCCTATTTTCCCTTTGTAATTAATAGTATATCTGCTAAGGAATTATGATTATGATTATAATTGTGAATTGTGATTGTCATACTGCATTTTGTAAAGAACTTTATCTGATATTAATCTTTCTAGGAAAACAAGTAACAAAAATAACATTTAGTATCCAAACTCTATTAGATATTGTATGAGGTTCGACTTAAGGAATCTCTGCAGTTGCACATCGATAGTGACTTGTAGGAAAGAAGACAAAAACACGTACAATAAGCAAATTTGAAACATGATTAGGTCATATGAATTTGTTTTAGTAATTGAACATATTTCAAATTAATTAATCTTTACAAAATTATTTATTGGGAAATGCTAACTTGTAATATTTGGGGCTTTATAGCAAGTTATATTATTATATGTTATCTAAATTGGTTTGGATTGGATTTACTTTTATGTATAGTGTGATCCAGAGCAACCGGGTTCTTGTGATGCCGGGTGCAATGGTGGTCTGATGAATAGTGCTTTTGAGTACATTTTAAAATCCGGTGGGGTGATGCGCGAAGAAGACTATCCTTATTCTGCTACTGATCGTGGAGCATGCAAGTTTGACAAGAAAAAAATTGCAGCATCAGTTGCAAATTTCAGCGTTGTTTCACTTGATGAAGACCAGATTGCCGCTAATCTTGTCAAGAACGGTCCTCTTGCTAGTAAGTGCTAAAGTCTTAACAATTTATGTTTTTTTGTTCAATTATAATTATCGAATTCAAATGCAGATATTGATTCTTGTAAATTTCAATGTGCACAAACGCAGTTGCCTTAAATGCAGTATATATGCAAACATACATTGGAGGAGTATCATGCCCATACATATGCTCAAAGAGACTAGATCATGGAGTGTTACTGGTGGGATATGGATCTGGAGCCTATTCGCCTATTAGGATGAAGGAGAAGCCATATTGGATAATTAAAAACTCATGGGGTGAGACTTGGGGAGAAAATGGATACTACAAAATATGCAGGGGACGAAATATATGTGGAGTGGACTCCATGGTTTCTACTGTGGCTGCCGTTCATACTACTACTCAGTAA
Protein sequence

>MS.gene003680.t1

MDHRTLLLFVVLFIFSVSAFSTPDDGEDSIIRQVVDDEGVRLGAEHHFNLFKHKFGKVYSSKDEHDYRFKIFKSNLNRAKKHQLMDPSAVHGVTKFSDLTPREFRQSVLGLRGVGLPKDANAAPILPTDNLPKDFDWREKGAVTAVKNQGSCGSCWSFSTTGALEGAHFLSTGKLVSLSEQQLVDCDHECDPEQPGSCDAGCNGGLMNSAFEYILKSGGVMREEDYPYSATDRGACKFDKKKIAASVANFSVVSLDEDQIAANLVKNGPLAIALNAVYMQTYIGGVSCPYICSKRLDHGVLLVGYGSGAYSPIRMKEKPYWIIKNSWGETWGENGYYKICRGRNICGVDSMVSTVAAVHTTTQ