AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031638

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031638.t1 MTR_4g079440 95.066 304 15 0 1 304 42 345 0.0 607
MS.gene031638.t1 MTR_2g090890 65.017 303 105 1 1 303 35 336 1.35e-149 423
MS.gene031638.t1 MTR_4g107930 57.468 308 125 3 1 302 36 343 5.55e-126 363
MS.gene031638.t1 MTR_5g022560 56.494 308 128 3 1 302 35 342 2.37e-123 357
MS.gene031638.t1 MTR_4g079470 56.209 306 127 4 3 302 39 343 2.89e-122 354
MS.gene031638.t1 MTR_4g079770 56.209 306 127 4 3 302 39 343 3.49e-122 353
MS.gene031638.t1 MTR_4g047610 55.519 308 131 3 1 302 34 341 1.26e-121 352
MS.gene031638.t1 MTR_4g079800 55.556 306 129 4 3 302 39 343 8.82e-120 348
MS.gene031638.t1 MTR_4g080730 53.947 304 137 3 1 302 36 338 2.68e-118 343
MS.gene031638.t1 MTR_4g080700 52.961 304 140 3 1 302 36 338 1.18e-116 340
MS.gene031638.t1 MTR_1g107550 54.341 311 133 5 1 303 46 355 4.87e-114 337
MS.gene031638.t1 MTR_3g075390 53.074 309 131 5 5 303 40 344 6.72e-114 333
MS.gene031638.t1 MTR_4g104370 54.723 307 128 6 5 303 40 343 7.08e-113 330
MS.gene031638.t1 MTR_2g075830 55.410 305 133 3 1 302 35 339 1.90e-111 326
MS.gene031638.t1 MTR_3g116080 52.318 302 138 3 5 302 47 346 1.36e-108 319
MS.gene031638.t1 MTR_4g094918 51.299 308 140 5 5 303 35 341 4.91e-108 318
MS.gene031638.t1 MTR_4g080360 50.000 310 147 5 1 303 33 341 2.21e-107 316
MS.gene031638.t1 MTR_2g083930 48.408 314 152 3 1 304 31 344 4.54e-107 315
MS.gene031638.t1 MTR_3g102220 51.592 314 140 7 1 303 49 361 8.54e-107 319
MS.gene031638.t1 MTR_1g018840 50.325 308 143 6 5 303 55 361 1.33e-102 308
MS.gene031638.t1 MTR_1g018840 50.325 308 143 6 5 303 55 361 1.44e-102 308
MS.gene031638.t1 MTR_3g112430 49.351 308 147 5 3 302 34 340 4.44e-101 303
MS.gene031638.t1 MTR_7g078600 49.511 307 146 7 1 304 38 338 9.79e-96 286
MS.gene031638.t1 MTR_7g079230 47.712 306 150 6 1 302 35 334 8.16e-91 274
MS.gene031638.t1 MTR_2g016180 43.322 307 162 5 5 302 51 354 3.35e-85 264
MS.gene031638.t1 MTR_2g016270 43.092 304 152 5 5 302 51 339 1.53e-83 259
MS.gene031638.t1 MTR_2g015850 45.734 293 144 7 21 304 78 364 2.04e-81 251
MS.gene031638.t1 MTR_2g015920 40.968 310 168 7 5 303 53 358 5.37e-73 229
MS.gene031638.t1 MTR_4g065077 41.214 313 163 11 5 303 51 356 1.90e-70 223
MS.gene031638.t1 MTR_4g125300 41.311 305 168 6 5 304 51 349 3.46e-69 219
MS.gene031638.t1 MTR_2g067900 41.905 315 160 9 4 301 15 323 1.47e-66 211
MS.gene031638.t1 MTR_1g023210 37.975 316 167 10 5 300 51 357 2.40e-66 212
MS.gene031638.t1 MTR_8g086470 39.159 309 159 10 5 303 67 356 3.31e-64 206
MS.gene031638.t1 MTR_2g067560 43.750 320 147 14 4 299 15 325 7.01e-64 216
MS.gene031638.t1 MTR_4g071060 37.061 313 173 8 5 300 48 353 9.09e-64 205
MS.gene031638.t1 MTR_2g064475 40.173 346 147 14 4 301 15 348 4.48e-63 203
MS.gene031638.t1 MTR_2g015930 37.898 314 173 9 5 303 52 358 6.33e-61 198
MS.gene031638.t1 MTR_1g094135 36.086 327 173 11 2 304 53 367 1.01e-59 195
MS.gene031638.t1 MTR_7g078630 39.630 270 126 5 1 259 702 945 7.10e-56 194
MS.gene031638.t1 MTR_2g066980 37.730 326 141 12 3 304 32 319 3.42e-55 182
MS.gene031638.t1 MTR_1g023810 41.743 218 119 5 87 301 49 261 2.27e-53 175
MS.gene031638.t1 MTR_4g072790 34.106 302 163 8 4 300 47 317 4.13e-48 164
MS.gene031638.t1 MTR_4g073660 33.333 306 164 8 4 300 46 320 3.87e-46 159
MS.gene031638.t1 MTR_4g072340 33.333 306 164 8 4 300 47 321 5.23e-46 158
MS.gene031638.t1 MTR_2g015840 32.667 300 136 11 8 304 36 272 4.01e-36 131
MS.gene031638.t1 MTR_7g079130 60.870 92 35 1 213 303 6 97 1.06e-32 116
MS.gene031638.t1 MTR_7g078580 55.556 99 42 2 206 303 4 101 2.34e-31 114
MS.gene031638.t1 MTR_1g025450 36.994 173 86 4 87 256 55 207 2.83e-30 120
MS.gene031638.t1 MTR_2g016380 42.478 113 61 2 193 302 23 134 1.11e-24 100
MS.gene031638.t1 MTR_7g078310 40.411 146 69 4 43 180 112 247 2.13e-24 99.8
MS.gene031638.t1 MTR_8g087510 41.912 136 72 5 128 261 23 153 2.16e-23 94.7
MS.gene031638.t1 MTR_3g029560 27.237 257 152 8 61 304 4 238 1.50e-22 94.4
MS.gene031638.t1 MTR_7g111050 27.972 286 163 13 50 302 61 336 1.24e-19 88.2
MS.gene031638.t1 MTR_7g111060 27.839 273 162 10 47 295 69 330 2.37e-19 87.4
MS.gene031638.t1 MTR_2g047960 51.250 80 38 1 96 175 7 85 1.19e-18 79.7
MS.gene031638.t1 MTR_7g079260 50.000 72 19 2 230 300 7 62 3.85e-15 70.5
MS.gene031638.t1 MTR_3g082370 28.636 220 129 9 92 294 5 213 1.97e-14 71.6
MS.gene031638.t1 MTR_7g111050 26.316 247 145 11 50 265 56 296 2.27e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031638.t1 AT1G06260 60.000 305 119 3 1 303 39 342 1.07e-130 375
MS.gene031638.t1 AT3G48340 56.494 308 123 5 5 303 38 343 2.52e-118 345
MS.gene031638.t1 AT5G43060 53.398 309 137 4 1 303 46 353 5.20e-114 337
MS.gene031638.t1 AT5G50260 54.369 309 127 5 5 303 38 342 1.61e-113 332
MS.gene031638.t1 AT5G45890 51.746 315 139 5 1 304 34 346 3.80e-113 331
MS.gene031638.t1 AT1G47128 52.303 304 139 5 5 303 50 352 3.45e-111 330
MS.gene031638.t1 AT4G35350 52.980 302 137 2 5 302 51 351 3.14e-110 324
MS.gene031638.t1 AT3G48350 52.090 311 134 6 3 302 36 342 7.07e-109 321
MS.gene031638.t1 AT1G20850 50.987 304 141 3 5 302 51 352 1.86e-105 311
MS.gene031638.t1 AT4G11310 50.327 306 143 5 5 303 49 352 6.75e-104 308
MS.gene031638.t1 AT4G11320 50.654 306 142 6 5 303 56 359 3.47e-103 306
MS.gene031638.t1 AT4G11320 50.654 306 142 6 5 303 56 359 3.47e-103 306
MS.gene031638.t1 AT4G36880 50.482 311 138 6 8 303 52 361 4.26e-102 304
MS.gene031638.t1 AT3G19390 47.712 306 154 4 2 302 40 344 1.71e-101 305
MS.gene031638.t1 AT4G23520 48.852 305 148 7 5 303 47 349 2.01e-101 301
MS.gene031638.t1 AT2G27420 49.371 318 141 7 3 303 33 347 5.04e-101 300
MS.gene031638.t1 AT3G19400 47.403 308 156 4 1 302 40 347 9.18e-101 300
MS.gene031638.t1 AT3G49340 49.521 313 141 8 3 303 33 340 1.71e-100 298
MS.gene031638.t1 AT1G09850 47.883 307 155 3 1 303 28 333 5.64e-99 298
MS.gene031638.t1 AT2G34080 46.006 313 156 6 1 303 35 344 5.37e-93 280
MS.gene031638.t1 AT1G29080 45.955 309 149 8 8 303 42 345 2.13e-87 265
MS.gene031638.t1 AT1G29090 45.338 311 149 8 8 303 50 354 2.28e-85 260
MS.gene031638.t1 AT3G43960 43.182 308 164 8 5 303 41 346 7.65e-79 244
MS.gene031638.t1 AT4G35350 50.000 242 115 3 1 238 47 286 8.55e-79 241
MS.gene031638.t1 AT1G29110 41.368 307 155 6 8 303 41 333 6.47e-75 233
MS.gene031638.t1 AT3G48350 47.036 253 120 5 3 245 36 284 7.61e-75 231
MS.gene031638.t1 AT5G60360 41.639 305 167 5 5 304 59 357 9.23e-73 228
MS.gene031638.t1 AT5G60360 41.311 305 167 6 5 304 59 356 2.62e-70 222
MS.gene031638.t1 AT3G19400 44.939 247 130 4 1 241 40 286 1.80e-69 218
MS.gene031638.t1 AT3G45310 39.344 305 174 6 5 304 59 357 2.77e-69 219
MS.gene031638.t1 AT5G60360 40.678 295 164 5 5 294 59 347 1.93e-67 214
MS.gene031638.t1 AT3G45310 39.016 305 174 7 5 304 59 356 9.66e-67 213
MS.gene031638.t1 AT4G39090 37.898 314 170 8 5 300 51 357 2.72e-66 212
MS.gene031638.t1 AT2G21430 36.570 309 171 8 5 295 48 349 1.14e-61 200
MS.gene031638.t1 AT3G54940 36.677 319 175 8 2 300 48 359 3.84e-58 191
MS.gene031638.t1 AT4G16190 36.508 315 175 8 5 300 55 363 3.97e-57 188
MS.gene031638.t1 AT3G54940 35.652 230 136 3 2 220 48 276 9.50e-41 143
MS.gene031638.t1 AT1G02305 28.671 286 156 12 43 295 63 333 7.01e-21 92.0
MS.gene031638.t1 AT4G01610 27.174 276 162 10 50 295 64 330 8.79e-21 91.7
MS.gene031638.t1 AT4G01610 26.812 276 163 10 50 295 64 330 1.47e-19 88.2
MS.gene031638.t1 AT1G02300 28.910 211 123 7 106 295 146 350 6.19e-15 75.1

Find 70 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTCCAATCCTTGCTTTC+TGG 0.072633 4.3:+29733148 None:intergenic
AGAGTTTCAAAGTACTTATA+TGG 0.243800 4.3:-29733233 MS.gene031638:CDS
ACCTTCAAACCTCTTCTTCA+TGG 0.288966 4.3:+29733411 None:intergenic
GACGAACGGGAAGTCCGTTT+TGG 0.317728 4.3:-29733352 MS.gene031638:CDS
CTTATATGGGTCTTGGTACT+AGG 0.338283 4.3:-29733219 MS.gene031638:CDS
TTTATTGTCTGTGAGATTGT+AGG 0.348908 4.3:+29733276 None:intergenic
AGGTTGCCAAGGTGGCTTAA+TGG 0.360502 4.3:-29732724 MS.gene031638:CDS
GGTGCTGACTGGGGTGAATC+TGG 0.360846 4.3:-29732360 MS.gene031638:CDS
GGCATCAACAAAATAAAATC+AGG 0.363474 4.3:-29732807 MS.gene031638:CDS
GTTCACATAATACAGGATTC+AGG 0.366790 4.3:-29733192 MS.gene031638:CDS
AGAATTCATGGGGTGCTGAC+TGG 0.387691 4.3:-29732371 MS.gene031638:CDS
GCTGCAGTGGCAGCTGTAGA+AGG 0.389218 4.3:-29732828 MS.gene031638:CDS
ATTGGATTGTGAAGAATTCA+TGG 0.392004 4.3:-29732383 MS.gene031638:CDS
CAAAGTACTTATATGGGTCT+TGG 0.395625 4.3:-29733226 MS.gene031638:CDS
GGATTCAGGTATGATGAAAA+TGG 0.398407 4.3:-29733178 MS.gene031638:CDS
TGTTCACACAGGAGGTTGTT+GGG 0.402344 4.3:-29732856 MS.gene031638:intron
AAGGAAATATTAATAAGTAT+TGG 0.416014 4.3:-29732401 MS.gene031638:CDS
GAATTCATGGGGTGCTGACT+GGG 0.423926 4.3:-29732370 MS.gene031638:CDS
GAAGAAGAGGTTTGAAGGTT+GGG 0.426580 4.3:-29733407 MS.gene031638:CDS
GGTGTAACCGTAGTTGGATA+TGG 0.429893 4.3:-29732426 MS.gene031638:CDS
GAGTTTCAAAGTACTTATAT+GGG 0.441325 4.3:-29733232 MS.gene031638:CDS
CATTATGCTGCGAGTATAAG+TGG 0.447078 4.3:-29732600 MS.gene031638:CDS
TATCCTTATGAAGGTGTAGA+TGG 0.448214 4.3:-29732648 MS.gene031638:CDS
AGCTTAGCCTCATTATCAGC+AGG 0.460600 4.3:+29732564 None:intergenic
AATCATGGTGTAACCGTAGT+TGG 0.476071 4.3:-29732432 MS.gene031638:CDS
ATACAAACATAATGACGAAC+GGG 0.476584 4.3:-29733365 MS.gene031638:CDS
TTAAGCCACCTTGGCAACCT+TGG 0.486289 4.3:+29732727 None:intergenic
GCCATGAAGAAGAGGTTTGA+AGG 0.488543 4.3:-29733412 MS.gene031638:CDS
AGGTTGCGTTCACATAATAC+AGG 0.488578 4.3:-29733199 MS.gene031638:CDS
TTGCTTGGTAAATGCCAAAA+CGG 0.493062 4.3:+29733338 None:intergenic
TGAAGAAGAGGTTTGAAGGT+TGG 0.494459 4.3:-29733408 MS.gene031638:CDS
GGGGTGAATCTGGTTATATC+AGG 0.495176 4.3:-29732350 MS.gene031638:CDS
GTGTTCACACAGGAGGTTGT+TGG 0.498927 4.3:-29732857 MS.gene031638:intron
TCCTTCTCCATATCCAACTA+CGG 0.504152 4.3:+29732419 None:intergenic
CGCGACACTTTGAGTAAAGA+AGG 0.504741 4.3:-29732321 MS.gene031638:CDS
AATAAGCTAAACTACACTAG+AGG 0.504746 4.3:+29732267 None:intergenic
ATCTTCCAGAAAGCAAGGAT+TGG 0.515492 4.3:-29733153 MS.gene031638:CDS
TCATTTCAGCTTTATTCAGA+AGG 0.521702 4.3:-29732486 MS.gene031638:CDS
AAACTACACTAGAGGGTAGC+TGG 0.525707 4.3:+29732275 None:intergenic
GTACCATCTACACCTTCATA+AGG 0.526767 4.3:+29732645 None:intergenic
TTCCAGAAAGCAAGGATTGG+AGG 0.532858 4.3:-29733150 MS.gene031638:CDS
GTAACTGAAATCAAGAATCA+AGG 0.536484 4.3:-29733115 MS.gene031638:CDS
TGTGGAAAGCAACTCAATCA+TGG 0.536875 4.3:-29732447 MS.gene031638:CDS
TGCATCAATTGCAACAGACA+CGG 0.539151 4.3:+29732518 None:intergenic
GTATGTATTGAACATTTGCT+TGG 0.546306 4.3:+29733323 None:intergenic
AGCAAGGATTGGAGGAAGGA+GGG 0.547902 4.3:-29733142 MS.gene031638:CDS
TTGTTGGGCATTCGCTGCAG+TGG 0.553618 4.3:-29732841 MS.gene031638:CDS
TGGTGATCTTCCAGAAAGCA+AGG 0.571198 4.3:-29733158 MS.gene031638:CDS
ACCGTAGTTGGATATGGAGA+AGG 0.573204 4.3:-29732420 MS.gene031638:CDS
AGAAGTTCCTGCTGATAATG+AGG 0.576412 4.3:-29732571 MS.gene031638:CDS
AATGGTAACCAAGGTTGCCA+AGG 0.576933 4.3:-29732735 MS.gene031638:CDS
AAGCAAGGATTGGAGGAAGG+AGG 0.583928 4.3:-29733143 MS.gene031638:CDS
GTGTCTGTTGCAATTGATGC+AGG 0.584191 4.3:-29732516 MS.gene031638:CDS
AATACAAACATAATGACGAA+CGG 0.584501 4.3:-29733366 MS.gene031638:CDS
CTGTCTCCATTAAGCCACCT+TGG 0.587016 4.3:+29732718 None:intergenic
ACGTAGAAGCCATGAAGAAG+AGG 0.591122 4.3:-29733420 None:intergenic
GAAGGTTGGGTGAAACGACA+TGG 0.591844 4.3:-29733394 MS.gene031638:CDS
GCATCAATTGCAACAGACAC+GGG 0.598881 4.3:+29732519 None:intergenic
GGTAACCAAGGTTGCCAAGG+TGG 0.606188 4.3:-29732732 MS.gene031638:CDS
ATAAGCTAAACTACACTAGA+GGG 0.610529 4.3:+29732268 None:intergenic
TTGGATTGTGAAGAATTCAT+GGG 0.612300 4.3:-29732382 MS.gene031638:CDS
AGAAAGCAAGGATTGGAGGA+AGG 0.613399 4.3:-29733146 MS.gene031638:CDS
GCATCAACAAAATAAAATCA+GGG 0.617083 4.3:-29732806 MS.gene031638:CDS
AATTCATGGGGTGCTGACTG+GGG 0.644464 4.3:-29732369 MS.gene031638:CDS
TGTAGATGGTACCTGTAAGA+CGG 0.648789 4.3:-29732634 MS.gene031638:CDS
TTGAGTAAAGAAGGTATGTG+TGG 0.649760 4.3:-29732312 MS.gene031638:CDS
TACAAGAAACATTACCACAC+TGG 0.663674 4.3:+29733092 None:intergenic
ATCAAGAATCAAGGCCAGTG+TGG 0.700928 4.3:-29733106 MS.gene031638:intron
TATACTCGCAGCATAATGTG+CGG 0.723834 4.3:+29732605 None:intergenic
TGGATTGTGAAGAATTCATG+GGG 0.729679 4.3:-29732381 MS.gene031638:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGGAAATATTAATAAGTAT+TGG - chr4.3:29733286-29733305 MS.gene031638:CDS 15.0%
!! ATATCAAGTTAAAAAAACAT+TGG + chr4.3:29732740-29732759 None:intergenic 15.0%
!! TATACATTCATCATAAAAAA+TGG - chr4.3:29732994-29733013 MS.gene031638:intron 15.0%
!! ATAGATTGTGATGTTAAAAA+TGG - chr4.3:29732934-29732953 MS.gene031638:intron 20.0%
! AATACAAACATAATGACGAA+CGG - chr4.3:29732321-29732340 MS.gene031638:CDS 25.0%
! ACATTCATCATAAAAAATGG+CGG - chr4.3:29732997-29733016 MS.gene031638:intron 25.0%
! ATGTGATTTCTTACAACTTA+AGG - chr4.3:29732699-29732718 MS.gene031638:CDS 25.0%
! GCATCAACAAAATAAAATCA+GGG - chr4.3:29732881-29732900 MS.gene031638:intron 25.0%
!! AGAGTTTCAAAGTACTTATA+TGG - chr4.3:29732454-29732473 MS.gene031638:CDS 25.0%
!! GAGTTTCAAAGTACTTATAT+GGG - chr4.3:29732455-29732474 MS.gene031638:CDS 25.0%
!! TTCATAAGGATAGTCTTTTT+CGG + chr4.3:29733031-29733050 None:intergenic 25.0%
!!! AACATTGGTATTTTAACTTG+TGG - chr4.3:29732767-29732786 MS.gene031638:CDS 25.0%
ATACAAACATAATGACGAAC+GGG - chr4.3:29732322-29732341 MS.gene031638:CDS 30.0%
ATAGCTTGAATCGCATAATA+TGG + chr4.3:29732672-29732691 None:intergenic 30.0%
ATTGGATTGTGAAGAATTCA+TGG - chr4.3:29733304-29733323 MS.gene031638:CDS 30.0%
CTTGATATTGTACGAAACAT+TGG - chr4.3:29732752-29732771 MS.gene031638:CDS 30.0%
GAAAAAGACTATCCTTATGA+AGG - chr4.3:29733030-29733049 MS.gene031638:intron 30.0%
GATGTTAAAAATGGTAACCA+AGG - chr4.3:29732943-29732962 MS.gene031638:intron 30.0%
GGCATCAACAAAATAAAATC+AGG - chr4.3:29732880-29732899 MS.gene031638:intron 30.0%
GTAACTGAAATCAAGAATCA+AGG - chr4.3:29732572-29732591 MS.gene031638:CDS 30.0%
GTATGTATTGAACATTTGCT+TGG + chr4.3:29732367-29732386 None:intergenic 30.0%
TTGGATTGTGAAGAATTCAT+GGG - chr4.3:29733305-29733324 MS.gene031638:CDS 30.0%
TTTATTGTCTGTGAGATTGT+AGG + chr4.3:29732414-29732433 None:intergenic 30.0%
TTTCTTGTAACAGAATTGCA+TGG - chr4.3:29732609-29732628 MS.gene031638:CDS 30.0%
!! TCATTTCAGCTTTATTCAGA+AGG - chr4.3:29733201-29733220 MS.gene031638:CDS 30.0%
GGATTCAGGTATGATGAAAA+TGG - chr4.3:29732509-29732528 MS.gene031638:CDS 35.0%
GTTCACATAATACAGGATTC+AGG - chr4.3:29732495-29732514 MS.gene031638:CDS 35.0%
TACAAGAAACATTACCACAC+TGG + chr4.3:29732598-29732617 None:intergenic 35.0%
TGGATTGTGAAGAATTCATG+GGG - chr4.3:29733306-29733325 MS.gene031638:CDS 35.0%
TTGCTTGGTAAATGCCAAAA+CGG + chr4.3:29732352-29732371 None:intergenic 35.0%
! AGAATTGCATGGCTTTTTAG+AGG - chr4.3:29732620-29732639 MS.gene031638:CDS 35.0%
! CAAAGTACTTATATGGGTCT+TGG - chr4.3:29732461-29732480 MS.gene031638:CDS 35.0%
! TATCCTTATGAAGGTGTAGA+TGG - chr4.3:29733039-29733058 MS.gene031638:intron 35.0%
! TTGAGTAAAGAAGGTATGTG+TGG - chr4.3:29733375-29733394 MS.gene031638:CDS 35.0%
!!! ATAAGGATAGTCTTTTTCGG+TGG + chr4.3:29733028-29733047 None:intergenic 35.0%
!!! CTTTTAAGTTGTGTTCACAC+AGG - chr4.3:29732820-29732839 MS.gene031638:CDS 35.0%
!!! TATTCAGAAGGTGTTTTCTC+TGG - chr4.3:29733213-29733232 MS.gene031638:CDS 35.0%
AATCATGGTGTAACCGTAGT+TGG - chr4.3:29733255-29733274 MS.gene031638:CDS 40.0%
AGGTTGCGTTCACATAATAC+AGG - chr4.3:29732488-29732507 MS.gene031638:CDS 40.0%
ATCTTCCAGAAAGCAAGGAT+TGG - chr4.3:29732534-29732553 MS.gene031638:CDS 40.0%
CATTATGCTGCGAGTATAAG+TGG - chr4.3:29733087-29733106 MS.gene031638:intron 40.0%
CTTATATGGGTCTTGGTACT+AGG - chr4.3:29732468-29732487 MS.gene031638:CDS 40.0%
GAAGAAGAGGTTTGAAGGTT+GGG - chr4.3:29732280-29732299 MS.gene031638:CDS 40.0%
GTACCATCTACACCTTCATA+AGG + chr4.3:29733045-29733064 None:intergenic 40.0%
TATACTCGCAGCATAATGTG+CGG + chr4.3:29733085-29733104 None:intergenic 40.0%
TCCTTCTCCATATCCAACTA+CGG + chr4.3:29733271-29733290 None:intergenic 40.0%
TGAAGAAGAGGTTTGAAGGT+TGG - chr4.3:29732279-29732298 MS.gene031638:CDS 40.0%
TGCATCAATTGCAACAGACA+CGG + chr4.3:29733172-29733191 None:intergenic 40.0%
TGTAGATGGTACCTGTAAGA+CGG - chr4.3:29733053-29733072 MS.gene031638:intron 40.0%
TGTGGAAAGCAACTCAATCA+TGG - chr4.3:29733240-29733259 MS.gene031638:CDS 40.0%
TGTGGAACATTCTCTCAAGA+TGG - chr4.3:29732785-29732804 MS.gene031638:CDS 40.0%
! AGAAGTTCCTGCTGATAATG+AGG - chr4.3:29733116-29733135 MS.gene031638:CDS 40.0%
! TTAAGTTGTGTTCACACAGG+AGG - chr4.3:29732823-29732842 MS.gene031638:CDS 40.0%
!! CATGGCTTTTTAGAGGTTCT+AGG - chr4.3:29732627-29732646 MS.gene031638:CDS 40.0%
AATGGTAACCAAGGTTGCCA+AGG - chr4.3:29732952-29732971 MS.gene031638:intron 45.0%
ACCGTAGTTGGATATGGAGA+AGG - chr4.3:29733267-29733286 MS.gene031638:CDS 45.0%
AGAAAGCAAGGATTGGAGGA+AGG - chr4.3:29732541-29732560 MS.gene031638:CDS 45.0%
AGCTTAGCCTCATTATCAGC+AGG + chr4.3:29733126-29733145 None:intergenic 45.0%
ATCAAGAATCAAGGCCAGTG+TGG - chr4.3:29732581-29732600 MS.gene031638:CDS 45.0%
CGCGACACTTTGAGTAAAGA+AGG - chr4.3:29733366-29733385 MS.gene031638:CDS 45.0%
GCATCAATTGCAACAGACAC+GGG + chr4.3:29733171-29733190 None:intergenic 45.0%
GGGGTGAATCTGGTTATATC+AGG - chr4.3:29733337-29733356 MS.gene031638:CDS 45.0%
GGTGTAACCGTAGTTGGATA+TGG - chr4.3:29733261-29733280 MS.gene031638:CDS 45.0%
GTGTCTGTTGCAATTGATGC+AGG - chr4.3:29733171-29733190 MS.gene031638:CDS 45.0%
TGGTGATCTTCCAGAAAGCA+AGG - chr4.3:29732529-29732548 MS.gene031638:CDS 45.0%
TGTTCACACAGGAGGTTGTT+GGG - chr4.3:29732831-29732850 MS.gene031638:CDS 45.0%
TTCCAGAAAGCAAGGATTGG+AGG - chr4.3:29732537-29732556 MS.gene031638:CDS 45.0%
TTCCTCCAATCCTTGCTTTC+TGG + chr4.3:29732542-29732561 None:intergenic 45.0%
! GGTGTTTTCTCTGGCATTTG+TGG - chr4.3:29733222-29733241 MS.gene031638:CDS 45.0%
AAGCAAGGATTGGAGGAAGG+AGG - chr4.3:29732544-29732563 MS.gene031638:CDS 50.0%
AGCAAGGATTGGAGGAAGGA+GGG - chr4.3:29732545-29732564 MS.gene031638:CDS 50.0%
AGGTTGCCAAGGTGGCTTAA+TGG - chr4.3:29732963-29732982 MS.gene031638:intron 50.0%
CTGTCTCCATTAAGCCACCT+TGG + chr4.3:29732972-29732991 None:intergenic 50.0%
GAAGGTTGGGTGAAACGACA+TGG - chr4.3:29732293-29732312 MS.gene031638:CDS 50.0%
GTGTTCACACAGGAGGTTGT+TGG - chr4.3:29732830-29732849 MS.gene031638:CDS 50.0%
TTAAGCCACCTTGGCAACCT+TGG + chr4.3:29732963-29732982 None:intergenic 50.0%
!! AATTCATGGGGTGCTGACTG+GGG - chr4.3:29733318-29733337 MS.gene031638:CDS 50.0%
!! AGAATTCATGGGGTGCTGAC+TGG - chr4.3:29733316-29733335 MS.gene031638:CDS 50.0%
!! GAATTCATGGGGTGCTGACT+GGG - chr4.3:29733317-29733336 MS.gene031638:CDS 50.0%
!! TGCGGCTTTTTCCGTCTTAC+AGG + chr4.3:29733067-29733086 None:intergenic 50.0%
GGTAACCAAGGTTGCCAAGG+TGG - chr4.3:29732955-29732974 MS.gene031638:intron 55.0%
TTGTTGGGCATTCGCTGCAG+TGG - chr4.3:29732846-29732865 MS.gene031638:CDS 55.0%
! GACGAACGGGAAGTCCGTTT+TGG - chr4.3:29732335-29732354 MS.gene031638:CDS 55.0%
GCTGCAGTGGCAGCTGTAGA+AGG - chr4.3:29732859-29732878 MS.gene031638:intron 60.0%
!! GGTGCTGACTGGGGTGAATC+TGG - chr4.3:29733327-29733346 MS.gene031638:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 29732278 29733431 29732278 ID=MS.gene031638
chr4.3 mRNA 29732278 29733431 29732278 ID=MS.gene031638.t1;Parent=MS.gene031638
chr4.3 exon 29733107 29733431 29733107 ID=MS.gene031638.t1.exon1;Parent=MS.gene031638.t1
chr4.3 CDS 29733107 29733431 29733107 ID=cds.MS.gene031638.t1;Parent=MS.gene031638.t1
chr4.3 exon 29732278 29732867 29732278 ID=MS.gene031638.t1.exon2;Parent=MS.gene031638.t1
chr4.3 CDS 29732278 29732867 29732278 ID=cds.MS.gene031638.t1;Parent=MS.gene031638.t1
Gene Sequence

>MS.gene031638

ATGAAGAAGAGGTTTGAAGGTTGGGTGAAACGACATGGTCGAAAATACAAACATAATGACGAACGGGAAGTCCGTTTTGGCATTTACCAAGCAAATGTTCAATACATACATTGCAAAAACGCACAGAAAAACTCCTACAATCTCACAGACAATAAATTTGCAGATCTTACGAATGAAGAGTTTCAAAGTACTTATATGGGTCTTGGTACTAGGTTGCGTTCACATAATACAGGATTCAGGTATGATGAAAATGGTGATCTTCCAGAAAGCAAGGATTGGAGGAAGGAGGGAGCAGTAACTGAAATCAAGAATCAAGGCCAGTGTGGTAATGTTTCTTGTAACAGAATTGCATGGCTTTTTAGAGGTTCTAGGTTATGCTATTTCAGTTTTACCATATTATGCGATTCAAGCTATGTTACTTATGTGATTTCTTACAACTTAAGGAACATTCTTACTCTGCCAATGTTTTTTTAACTTGATATTGTACGAAACATTGGTATTTTAACTTGTGGAACATTCTCTCAAGATGGTTGTTTTAACTACTTTTAAGTTGTGTTCACACAGGAGGTTGTTGGGCATTCGCTGCAGTGGCAGCTGTAGAAGGCATCAACAAAATAAAATCAGGGAAATTGATCTCTCTATCTGAACAAGAACTAATAGATTGTGATGTTAAAAATGGTAACCAAGGTTGCCAAGGTGGCTTAATGGAGACAGCATATACATTCATCATAAAAAATGGCGGACTAACCACCGAAAAAGACTATCCTTATGAAGGTGTAGATGGTACCTGTAAGACGGAAAAAGCCGCACATTATGCTGCGAGTATAAGTGGATATGAAGAAGTTCCTGCTGATAATGAGGCTAAGCTTAAAGCTGCTGCAGCTCATCAACCCGTGTCTGTTGCAATTGATGCAGGAAGTTATTCATTTCAGCTTTATTCAGAAGGTGTTTTCTCTGGCATTTGTGGAAAGCAACTCAATCATGGTGTAACCGTAGTTGGATATGGAGAAGGAAATATTAATAAGTATTGGATTGTGAAGAATTCATGGGGTGCTGACTGGGGTGAATCTGGTTATATCAGGATGAAGCGCGACACTTTGAGTAAAGAAGGTATGTGTGGCATTGCTATGCAAGCCAGCTACCCTCTAGTGTAG
Protein sequence

>MS.gene031638.t1

MKKRFEGWVKRHGRKYKHNDEREVRFGIYQANVQYIHCKNAQKNSYNLTDNKFADLTNEEFQSTYMGLGTRLRSHNTGFRYDENGDLPESKDWRKEGAVTEIKNQGQCGGCWAFAAVAAVEGINKIKSGKLISLSEQELIDCDVKNGNQGCQGGLMETAYTFIIKNGGLTTEKDYPYEGVDGTCKTEKAAHYAASISGYEEVPADNEAKLKAAAAHQPVSVAIDAGSYSFQLYSEGVFSGICGKQLNHGVTVVGYGEGNINKYWIVKNSWGADWGESGYIRMKRDTLSKEGMCGIAMQASYPLV