AlfalfaGEDB | MS.gene50738

M. sativa cultivar XinJiangDaYe / MS.gene50738

KEGG: K01373
GO: GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00112.26 PF08246.15 PF13976.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50738.t1	XP_003606998.1	91.5	329	8	1	222	530	34	362	1.10E-174	623.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50738.t1	P43295	70.8	339	75	3	209	525	22	358	5.1e-141	502.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50738.t1	G7JIL2	91.5	329	8	1	222	530	34	362	7.8e-175	623.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050945	MS.gene50738	0.808581	2.89E-50	-1.69E-46
MS.gene054004	MS.gene50738	0.810128	1.34E-50	-1.69E-46
MS.gene055150	MS.gene50738	0.803248	3.82E-49	-1.69E-46
MS.gene055886	MS.gene50738	0.835907	1.28E-56	-1.69E-46
MS.gene059949	MS.gene50738	0.823158	1.61E-53	-1.69E-46
MS.gene060244	MS.gene50738	0.810964	8.87E-51	-1.69E-46
MS.gene060522	MS.gene50738	0.820624	6.22E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene50738	MS.gene045525	PPI
MS.gene069978	MS.gene50738	PPI
MS.gene50738	MS.gene006665	PPI
MS.gene50738	MS.gene006680	PPI
MS.gene51064	MS.gene50738	PPI
MS.gene026876	MS.gene50738	PPI
MS.gene014304	MS.gene50738	PPI
MS.gene24818	MS.gene50738	PPI
MS.gene23321	MS.gene50738	PPI
MS.gene69195	MS.gene50738	PPI
MS.gene50738	MS.gene003677	PPI
MS.gene50738	MS.gene26670	PPI
MS.gene50738	MS.gene63719	PPI
MS.gene50738	MS.gene005934	PPI
MS.gene50738	MS.gene26669	PPI
MS.gene50738	MS.gene78715	PPI
MS.gene63396	MS.gene50738	PPI
MS.gene003680	MS.gene50738	PPI
MS.gene50738	MS.gene73817	PPI
MS.gene50738	MS.gene011363	PPI
MS.gene50738	MS.gene052628	PPI
MS.gene028850	MS.gene50738	PPI
MS.gene50738	MS.gene64795	PPI
MS.gene064873	MS.gene50738	PPI
MS.gene50738	MS.gene73835	PPI
MS.gene031580	MS.gene50738	PPI
MS.gene26853	MS.gene50738	PPI
MS.gene50738	MS.gene045461	PPI
MS.gene027420	MS.gene50738	PPI
MS.gene50738	MS.gene065787	PPI
MS.gene50738	MS.gene033324	PPI
MS.gene73834	MS.gene50738	PPI
MS.gene50738	MS.gene09529	PPI
MS.gene005933	MS.gene50738	PPI
MS.gene006682	MS.gene50738	PPI
MS.gene052630	MS.gene50738	PPI
MS.gene73815	MS.gene50738	PPI
MS.gene29082	MS.gene50738	PPI
MS.gene50738	MS.gene045455	PPI
MS.gene045459	MS.gene50738	PPI
MS.gene50738	MS.gene031638	PPI
MS.gene50738	MS.gene26853	PPI
MS.gene66833	MS.gene50738	PPI
MS.gene50738	MS.gene045526	PPI
MS.gene50738	MS.gene045520	PPI
MS.gene50738	MS.gene67468	PPI
MS.gene50738	MS.gene73838	PPI
MS.gene50738	MS.gene045503	PPI
MS.gene033151	MS.gene50738	PPI
MS.gene50738	MS.gene043473	PPI
MS.gene50738	MS.gene031581	PPI
MS.gene50738	MS.gene48296	PPI
MS.gene50738	MS.gene73840	PPI
MS.gene031635	MS.gene50738	PPI
MS.gene055760	MS.gene50738	PPI
MS.gene065785	MS.gene50738	PPI
MS.gene022410	MS.gene50738	PPI
MS.gene50738	MS.gene045522	PPI
MS.gene060218	MS.gene50738	PPI
MS.gene50738	MS.gene25255	PPI
MS.gene026874	MS.gene50738	PPI
MS.gene59493	MS.gene50738	PPI
MS.gene50738	MS.gene87639	PPI
MS.gene34090	MS.gene50738	PPI
MS.gene50738	MS.gene031619	PPI
MS.gene50738	MS.gene031621	PPI
MS.gene026873	MS.gene50738	PPI
MS.gene50738	MS.gene058156	PPI
MS.gene50738	MS.gene31410	PPI
MS.gene006646	MS.gene50738	PPI
MS.gene012206	MS.gene50738	PPI
MS.gene50738	MS.gene045458	PPI
MS.gene95543	MS.gene50738	PPI
MS.gene059966	MS.gene50738	PPI
MS.gene50738	MS.gene006720	PPI
MS.gene50738	MS.gene045501	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50738.t1	MTR_4g071060	91.489	329	8	1	222	530	34	362	0.0	630
MS.gene50738.t1	MTR_1g023210	70.303	330	75	3	221	528	36	364	1.87e-171	489
MS.gene50738.t1	MTR_4g072340	65.109	321	60	4	231	531	43	331	9.08e-146	422
MS.gene50738.t1	MTR_4g073660	65.109	321	60	4	231	531	42	330	9.16e-146	422
MS.gene50738.t1	MTR_4g072790	66.149	322	51	5	231	531	43	327	1.60e-145	421
MS.gene50738.t1	MTR_1g094135	55.319	329	122	4	223	528	44	370	8.66e-131	386
MS.gene50738.t1	MTR_4g047610	39.542	306	139	9	242	516	44	334	1.53e-61	206
MS.gene50738.t1	MTR_4g079470	39.809	314	143	10	233	516	39	336	8.01e-61	204
MS.gene50738.t1	MTR_4g079770	40.132	304	137	9	243	516	48	336	1.60e-60	203
MS.gene50738.t1	MTR_4g079800	38.558	319	150	10	233	521	39	341	6.10e-60	202
MS.gene50738.t1	MTR_5g022560	39.048	315	145	10	233	516	37	335	1.20e-59	201
MS.gene50738.t1	MTR_1g107550	40.667	300	132	7	238	509	54	335	1.71e-59	204
MS.gene50738.t1	MTR_4g125300	40.000	310	147	8	236	521	51	345	2.66e-59	200
MS.gene50738.t1	MTR_4g094918	40.129	309	122	10	238	509	39	321	3.46e-59	200
MS.gene50738.t1	MTR_3g116080	37.658	316	149	9	236	521	47	344	1.46e-57	196
MS.gene50738.t1	MTR_4g080700	36.792	318	156	9	226	516	32	331	2.12e-57	195
MS.gene50738.t1	MTR_4g107930	38.889	306	141	9	242	516	46	336	4.01e-57	194
MS.gene50738.t1	MTR_4g079440	36.156	307	154	9	236	516	46	336	1.24e-56	193
MS.gene50738.t1	MTR_4g080730	36.755	302	148	8	242	516	46	331	1.86e-56	192
MS.gene50738.t1	MTR_3g102220	36.709	316	149	7	225	509	46	341	5.24e-56	195
MS.gene50738.t1	MTR_1g018840	39.482	309	124	10	238	509	59	341	2.47e-55	193
MS.gene50738.t1	MTR_1g018840	39.482	309	124	10	238	509	59	341	2.60e-55	192
MS.gene50738.t1	MTR_3g112430	37.383	321	148	11	236	521	36	338	1.64e-54	190
MS.gene50738.t1	MTR_3g075390	37.124	299	139	9	251	516	54	336	1.02e-53	186
MS.gene50738.t1	MTR_2g075830	37.333	300	147	7	244	516	47	332	2.58e-53	184
MS.gene50738.t1	MTR_4g080360	35.505	307	150	9	242	516	43	333	2.65e-53	184
MS.gene50738.t1	MTR_4g104370	37.171	304	141	9	246	516	49	335	8.22e-53	183
MS.gene50738.t1	MTR_2g090890	35.161	310	161	7	242	527	45	338	1.18e-50	177
MS.gene50738.t1	MTR_2g016270	36.601	306	155	11	236	521	51	337	6.49e-50	178
MS.gene50738.t1	MTR_2g015850	37.324	284	135	7	252	509	78	344	9.02e-49	172
MS.gene50738.t1	MTR_2g016180	35.514	321	153	12	236	521	51	352	3.65e-48	174
MS.gene50738.t1	MTR_2g015920	33.333	324	151	11	236	518	53	352	4.39e-47	168
MS.gene50738.t1	MTR_4g065077	34.627	335	154	13	225	520	44	352	4.92e-47	168
MS.gene50738.t1	MTR_2g083930	35.220	318	155	12	244	526	43	344	1.11e-46	166
MS.gene50738.t1	MTR_7g078600	32.602	319	178	9	220	516	26	329	1.05e-44	161
MS.gene50738.t1	MTR_8g086470	33.537	328	151	12	225	520	60	352	1.18e-43	159
MS.gene50738.t1	MTR_2g015930	31.902	326	152	11	236	517	52	351	2.29e-41	152
MS.gene50738.t1	MTR_8g087510	50.735	136	56	3	340	474	23	148	8.85e-41	145
MS.gene50738.t1	MTR_2g067900	33.333	339	159	14	227	521	7	322	3.16e-40	148
MS.gene50738.t1	MTR_7g079230	31.667	300	174	8	224	516	52	327	9.36e-40	147
MS.gene50738.t1	MTR_1g023810	41.395	215	106	7	304	515	54	251	2.21e-37	139
MS.gene50738.t1	MTR_2g064475	35.366	246	119	11	298	530	134	352	7.07e-35	134
MS.gene50738.t1	MTR_2g067560	31.818	330	152	12	227	509	7	310	1.39e-32	133
MS.gene50738.t1	MTR_2g066980	28.363	342	148	13	227	523	26	315	3.35e-27	112
MS.gene50738.t1	MTR_7g078630	29.921	254	150	6	236	476	706	944	5.45e-25	110
MS.gene50738.t1	MTR_7g111050	30.800	250	133	10	297	521	98	332	3.08e-20	92.8
MS.gene50738.t1	MTR_7g111060	29.344	259	145	9	288	522	92	336	7.03e-20	91.7
MS.gene50738.t1	MTR_1g025450	36.559	186	89	7	292	474	48	207	1.45e-18	89.4
MS.gene50738.t1	MTR_7g078310	42.857	98	47	2	304	399	157	247	1.26e-14	74.3
MS.gene50738.t1	MTR_2g047960	42.391	92	45	1	303	394	2	85	2.76e-14	69.3
MS.gene50738.t1	MTR_2g015840	33.333	141	64	3	242	352	39	179	1.71e-13	71.2
MS.gene50738.t1	MTR_2g015840	34.746	118	69	3	412	528	161	271	2.85e-13	70.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50738.t1	AT2G21430	70.588	340	76	3	209	526	22	359	7.27e-179	508
MS.gene50738.t1	AT4G39090	71.299	331	74	1	221	530	36	366	8.11e-178	505
MS.gene50738.t1	AT4G16190	69.605	329	77	3	224	529	43	371	5.49e-165	473
MS.gene50738.t1	AT3G54940	59.159	333	106	5	221	524	31	362	1.12e-140	410
MS.gene50738.t1	AT3G54940	54.251	247	84	4	221	439	31	276	2.43e-87	271
MS.gene50738.t1	AT3G45310	40.810	321	144	10	230	521	50	353	4.37e-63	210
MS.gene50738.t1	AT5G60360	40.187	321	146	10	230	521	50	353	5.88e-63	210
MS.gene50738.t1	AT3G19400	38.973	331	148	11	224	521	36	345	3.93e-62	208
MS.gene50738.t1	AT5G60360	40.635	315	141	10	230	515	50	347	1.05e-61	207
MS.gene50738.t1	AT3G45310	40.764	314	140	10	230	514	50	346	1.18e-61	206
MS.gene50738.t1	AT5G60360	40.446	314	141	10	230	514	50	346	2.56e-61	206
MS.gene50738.t1	AT3G19390	38.304	342	157	11	215	521	20	342	2.06e-59	203
MS.gene50738.t1	AT1G20850	39.308	318	143	9	236	521	51	350	2.80e-59	200
MS.gene50738.t1	AT1G09850	38.080	323	151	10	236	527	32	336	8.30e-59	201
MS.gene50738.t1	AT1G47128	39.527	296	139	6	238	509	54	333	1.33e-58	201
MS.gene50738.t1	AT5G43060	41.135	282	125	8	252	509	70	334	2.97e-57	198
MS.gene50738.t1	AT3G43960	37.963	324	158	8	215	509	18	327	2.36e-56	193
MS.gene50738.t1	AT4G36880	38.062	289	132	6	252	509	69	341	5.51e-55	189
MS.gene50738.t1	AT4G35350	36.392	316	154	9	236	521	51	349	5.58e-55	189
MS.gene50738.t1	AT5G45890	32.873	362	180	9	190	516	4	337	6.69e-55	188
MS.gene50738.t1	AT1G29090	38.006	321	145	12	232	516	44	346	7.32e-55	189
MS.gene50738.t1	AT1G06260	37.700	313	134	13	245	521	52	339	1.29e-53	185
MS.gene50738.t1	AT3G48340	36.503	326	154	9	218	509	18	324	5.30e-53	184
MS.gene50738.t1	AT4G23520	34.650	329	161	12	225	521	40	346	3.98e-52	181
MS.gene50738.t1	AT2G27420	34.810	316	148	10	242	516	41	339	1.68e-51	179
MS.gene50738.t1	AT5G50260	37.821	312	129	10	246	516	47	334	2.01e-51	179
MS.gene50738.t1	AT3G48350	37.671	292	122	9	256	510	57	325	1.43e-49	175
MS.gene50738.t1	AT1G29080	35.669	314	152	9	242	521	45	342	4.27e-49	173
MS.gene50738.t1	AT3G49340	33.546	313	155	10	242	519	41	335	2.21e-48	171
MS.gene50738.t1	AT2G34080	35.370	311	146	11	242	516	45	336	6.44e-48	170
MS.gene50738.t1	AT4G11310	32.213	357	185	9	199	516	6	344	8.44e-47	167
MS.gene50738.t1	AT3G19400	38.211	246	116	6	224	444	36	270	5.27e-46	163
MS.gene50738.t1	AT4G11320	33.333	315	157	8	236	516	56	351	4.04e-45	163
MS.gene50738.t1	AT4G11320	33.333	315	157	8	236	516	56	351	4.04e-45	163
MS.gene50738.t1	AT4G35350	35.193	233	118	5	236	444	51	274	8.88e-40	146
MS.gene50738.t1	AT1G29110	31.013	316	153	9	242	521	44	330	1.23e-39	147
MS.gene50738.t1	AT3G48350	35.146	239	102	6	256	455	57	281	7.79e-35	133
MS.gene50738.t1	AT1G02305	30.800	250	131	11	297	520	104	337	2.95e-19	90.1
MS.gene50738.t1	AT4G01610	30.213	235	126	10	305	517	113	331	3.53e-19	89.7
MS.gene50738.t1	AT4G01610	29.787	235	127	10	305	517	113	331	5.80e-18	85.9
MS.gene50738.t1	AT1G02300	29.778	225	120	11	318	520	146	354	5.96e-17	83.2

Find 120 sgRNAs with CRISPR-Local

Find 367 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCATATATACTGCATTTA+AGG	0.130654	4.3:+34227936	None:intergenic
TTCTTTATTGGTTGTCTCTA+TGG	0.155563	4.3:+34230921	None:intergenic
AACCTATTTAAACATAAGTT+TGG	0.234066	4.3:-34229261	MS.gene50738:CDS
TCCCAAACTTATGTTTAAAT+AGG	0.268902	4.3:+34229259	None:intergenic
TGCTCATCTCTTCTTTCTCT+TGG	0.271304	4.3:+34230509	None:intergenic
CCAAGGATGAACATGATTAC+AGG	0.304516	4.3:-34229224	MS.gene50738:CDS
GGTGAGACTTGGGGAGAAAA+TGG	0.310928	4.3:-34227772	MS.gene50738:CDS
CTGGAGCCTATTCGCCTATT+AGG	0.320950	4.3:-34227834	MS.gene50738:CDS
GGAGTTTCGTCAGAGCGTTT+TGG	0.321592	4.3:-34229100	MS.gene50738:CDS
CCTGAGAAGTATCTGCTTGA+TGG	0.345792	4.3:+34230994	None:intergenic
CCACATGAGCGAGAAAAGTT+TGG	0.355767	4.3:-34232063	MS.gene50738:CDS
GAAGAAGACTATCCTTATTC+TGG	0.363696	4.3:-34228191	MS.gene50738:CDS
CCACGATCAGTACCAGAATA+AGG	0.365501	4.3:+34228179	None:intergenic
ACTAGATCATGGAGTGTTAC+TGG	0.371273	4.3:-34227869	MS.gene50738:CDS
TCTTTCTCTTGGTCATTCTC+AGG	0.376250	4.3:+34230520	None:intergenic
ACCTATTTAAACATAAGTTT+GGG	0.393244	4.3:-34229260	MS.gene50738:CDS
TTCAATGCCCTCTTTCCATC+TGG	0.395147	4.3:+34230826	None:intergenic
AGTGGTTGACCCTTGATGTT+TGG	0.395213	4.3:+34232017	None:intergenic
AGTCTCTTTGAGCATATGTA+TGG	0.401014	4.3:+34227889	None:intergenic
TATGTAAACTTCACTGAGAA+AGG	0.406154	4.3:-34230967	MS.gene50738:CDS
ATGGCTTCCATGAAGCTTTG+AGG	0.406974	4.3:+34230940	None:intergenic
GGCTTCTCCTTCATCCTAAT+AGG	0.407412	4.3:+34227820	None:intergenic
TCACAAGAACCCGGTTGCTC+TGG	0.408096	4.3:+34228284	None:intergenic
GGAGTGTTACTGGTGGGATA+TGG	0.410428	4.3:-34227859	MS.gene50738:CDS
GCTACCCACAGCTGAAGAAT+AGG	0.411440	4.3:+34230480	None:intergenic
TAAAATTACCAGATGGAAAG+AGG	0.419809	4.3:-34230834	MS.gene50738:CDS
GGATGAAGGAGAAGCCATAT+TGG	0.423435	4.3:-34227813	MS.gene50738:CDS
TGCTAAGAAGCATCAATTGA+TGG	0.425569	4.3:-34229172	MS.gene50738:CDS
AGGCTCTCTGTATGTTATGC+AGG	0.426835	4.3:-34232156	MS.gene50738:CDS
CTCGTTGATCTTCTCAATTG+AGG	0.429167	4.3:+34231027	None:intergenic
CTTCATCCTAATAGGCGAAT+AGG	0.432818	4.3:+34227828	None:intergenic
TGACAAGATTAGCGGCAATC+TGG	0.437809	4.3:+34228084	None:intergenic
CAACCGGGTTCTTGTGATGC+CGG	0.438520	4.3:-34228278	MS.gene50738:CDS
ATAGTGTGATCCAGAGCAAC+CGG	0.450276	4.3:-34228294	MS.gene50738:intron
TCTTGTGATGCCGGGTGCAA+TGG	0.456716	4.3:-34228269	MS.gene50738:CDS
CCTGTAATCATGTTCATCCT+TGG	0.456777	4.3:+34229224	None:intergenic
AAAACTCATGGGGTGAGACT+TGG	0.466256	4.3:-34227783	MS.gene50738:CDS
GCATCAATTGATGGATCCTT+CGG	0.466749	4.3:-34229163	MS.gene50738:CDS
TGTTGGAGTTTCAGTACAAC+CGG	0.469204	4.3:-34228841	MS.gene50738:CDS
ATGGATCCTTCGGCGGTTCA+CGG	0.470073	4.3:-34229153	MS.gene50738:CDS
AAACTCATGGGGTGAGACTT+GGG	0.470356	4.3:-34227782	MS.gene50738:CDS
ATGGATACTACAAAATATGC+AGG	0.476628	4.3:-34227753	MS.gene50738:CDS
CCTGTGCATGTTCCGTTACC+TGG	0.477809	4.3:-34230571	MS.gene50738:CDS
CTACCCACAGCTGAAGAATA+GGG	0.479660	4.3:+34230481	None:intergenic
AGGGGAACCTCAAAGCTTCA+TGG	0.481569	4.3:-34230947	MS.gene50738:CDS
AACCGGGTTCTTGTGATGCC+GGG	0.494687	4.3:-34228277	MS.gene50738:CDS
AGATTGCATCTCTTCTTCCA+TGG	0.498414	4.3:+34230885	None:intergenic
ATGTAAACTTCACTGAGAAA+GGG	0.498898	4.3:-34230966	MS.gene50738:CDS
TGCAGACCATGGCGTACATA+AGG	0.500035	4.3:+34230458	None:intergenic
TTACTGGTGGGATATGGATC+TGG	0.507620	4.3:-34227853	MS.gene50738:CDS
ATCTCAAGAAAGTTCTTGAA+AGG	0.511089	4.3:-34230615	MS.gene50738:CDS
TCAATTGATGCTTCTTAGCA+CGG	0.511562	4.3:+34229175	None:intergenic
GACGATGAAGAAGTGCGGTT+GGG	0.513257	4.3:-34229300	MS.gene50738:CDS
GAATCATCTTCCAAACATCA+AGG	0.513638	4.3:-34232027	MS.gene50738:CDS
TGGGAAGGTTTATTCTTCCA+AGG	0.519917	4.3:-34229241	MS.gene50738:CDS
GGTGCTCACTTTCTCTCGAC+CGG	0.523916	4.3:-34228808	MS.gene50738:CDS
GGTGCCCTATTCTTCAGCTG+TGG	0.526340	4.3:-34230485	MS.gene50738:CDS
CCTACAGCATAACCGATGTC+TGG	0.542961	4.3:+34230430	None:intergenic
CCTTATTCTGGTACTGATCG+TGG	0.543158	4.3:-34228179	MS.gene50738:CDS
CGACGATGAAGAAGTGCGGT+TGG	0.543666	4.3:-34229301	MS.gene50738:CDS
CTTTAGCACTTACTAGCAAG+AGG	0.547021	4.3:+34228053	None:intergenic
ACCGTTCTTGACAAGATTAG+CGG	0.547122	4.3:+34228076	None:intergenic
GTGCCCTATTCTTCAGCTGT+GGG	0.550386	4.3:-34230484	MS.gene50738:CDS
CCATCAAGCAGATACTTCTC+AGG	0.552246	4.3:-34230994	MS.gene50738:CDS
AGTACAACCGGTGCACTTGA+AGG	0.552820	4.3:-34228829	MS.gene50738:CDS
AAACTCCCTCGGAGTAAGAT+CGG	0.553683	4.3:+34229116	None:intergenic
CCAGGTAACGGAACATGCAC+AGG	0.555545	4.3:+34230571	None:intergenic
TTCTTCGCGCATCACCCCAC+CGG	0.555594	4.3:+34228208	None:intergenic
CCAGACATCGGTTATGCTGT+AGG	0.556943	4.3:-34230430	MS.gene50738:CDS
CATCAGACCACCATTGCACC+CGG	0.562811	4.3:+34228259	None:intergenic
GTCTGGCCTCGTGCAGACCA+TGG	0.563338	4.3:+34230447	None:intergenic
CTATTCGCCTATTAGGATGA+AGG	0.564118	4.3:-34227827	MS.gene50738:CDS
GAACTGTGGCACAAACGACT+TGG	0.565258	4.3:-34232086	MS.gene50738:CDS
TGCTCAAAGAGACTAGATCA+TGG	0.566764	4.3:-34227880	MS.gene50738:CDS
TGTAAACTTCACTGAGAAAG+GGG	0.577439	4.3:-34230965	MS.gene50738:CDS
GGCTCTCTGTATGTTATGCA+GGG	0.583723	4.3:-34232155	MS.gene50738:CDS
GTCTCTTTGAGCATATGTAT+GGG	0.587803	4.3:+34227890	None:intergenic
ATTTAAACATAAGTTTGGGA+AGG	0.587990	4.3:-34229256	MS.gene50738:CDS
GCCGCTAATCTTGTCAAGAA+CGG	0.589186	4.3:-34228077	MS.gene50738:CDS
AAAATTACCAGATGGAAAGA+GGG	0.591408	4.3:-34230833	MS.gene50738:CDS
AATCATCTTCCAAACATCAA+GGG	0.591800	4.3:-34232026	MS.gene50738:intron
GTGAGCACCTTCAAGTGCAC+CGG	0.592969	4.3:+34228822	None:intergenic
AACTCATGGGGTGAGACTTG+GGG	0.593059	4.3:-34227781	MS.gene50738:CDS
TTACTAAGTTTCATATGACC+AGG	0.594545	4.3:+34230553	None:intergenic
ACTTGTCGATTGTGATCATG+AGG	0.595475	4.3:-34228758	MS.gene50738:intron
TGGATACTACAAAATATGCA+GGG	0.599253	4.3:-34227752	MS.gene50738:CDS
GTATATATGCAAACATACAT+TGG	0.601664	4.3:-34227925	MS.gene50738:CDS
TGTGATGCCGGGTGCAATGG+TGG	0.601772	4.3:-34228266	MS.gene50738:CDS
TATATGCGGAGTGGACTCCA+TGG	0.603926	4.3:-34227722	MS.gene50738:CDS
ACGCTCTGACGAAACTCCCT+CGG	0.605558	4.3:+34229105	None:intergenic
ATTCTCCGATCTTACTCCGA+GGG	0.607597	4.3:-34229121	MS.gene50738:CDS
CACCCGGCATCACAAGAACC+CGG	0.609271	4.3:+34228275	None:intergenic
TATATGCAAACATACATTGG+AGG	0.612222	4.3:-34227922	MS.gene50738:CDS
GGGTAGCCTTATGTACGCCA+TGG	0.613486	4.3:-34230464	MS.gene50738:CDS
CTACTGAGTAGTAGTATGAA+CGG	0.624053	4.3:+34227684	None:intergenic
GGACTCCATGGTTTCTACTG+TGG	0.626354	4.3:-34227710	MS.gene50738:CDS
GACCTGGTAAAATTACCAGA+TGG	0.629718	4.3:-34230841	MS.gene50738:CDS
AGAGAAAGAAGAGATGAGCA+AGG	0.630245	4.3:-34230506	MS.gene50738:CDS
TCAATTGATGGATCCTTCGG+CGG	0.633662	4.3:-34229160	MS.gene50738:CDS
GATCATGGAGTGTTACTGGT+GGG	0.636411	4.3:-34227865	MS.gene50738:CDS
GTAACACCGTGAACCGCCGA+AGG	0.638844	4.3:+34229147	None:intergenic
TAGTGTGATCCAGAGCAACC+GGG	0.639888	4.3:-34228293	MS.gene50738:CDS
AGGGCTCGTGTGGATCATGT+TGG	0.640660	4.3:-34228858	MS.gene50738:CDS
GTCTGCACGAGGCCAGACAT+CGG	0.643214	4.3:-34230442	MS.gene50738:CDS
ATCATGAATTAGGGCTCGTG+TGG	0.646039	4.3:-34228868	MS.gene50738:intron
GGCAGCCACAGTAGAAACCA+TGG	0.655273	4.3:+34227705	None:intergenic
AGTTTCATATGACCAGGTAA+CGG	0.656108	4.3:+34230559	None:intergenic
ATCGTTCGAGAAGAATGTTG+TGG	0.657117	4.3:-34230654	MS.gene50738:CDS
TATGCAGGGAAAGATAAACA+AGG	0.666815	4.3:-34232141	MS.gene50738:CDS
AGATCATGGAGTGTTACTGG+TGG	0.667806	4.3:-34227866	MS.gene50738:CDS
GGATACTACAAAATATGCAG+GGG	0.670950	4.3:-34227751	MS.gene50738:CDS
ATGCAGGGAAAGATAAACAA+GGG	0.675651	4.3:-34232140	MS.gene50738:CDS
AGAGAACCAGACGTATGACC+TGG	0.678044	4.3:-34230857	MS.gene50738:CDS
TGCAGGGGACGAAATATATG+CGG	0.679393	4.3:-34227736	MS.gene50738:CDS
GGGACGAAATATATGCGGAG+TGG	0.687467	4.3:-34227731	MS.gene50738:CDS
GGAAGCATGATCATTGCTCG+TGG	0.693757	4.3:-34232188	None:intergenic
TGTACGCCATGGTCTGCACG+AGG	0.697121	4.3:-34230453	MS.gene50738:CDS
AATTCTCCGATCTTACTCCG+AGG	0.702571	4.3:-34229122	MS.gene50738:CDS
TGGTCGACGATGAAGAAGTG+CGG	0.727360	4.3:-34229305	MS.gene50738:CDS
AAGACATGAGCAAAGAACTG+TGG	0.731330	4.3:-34232100	MS.gene50738:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATTATATGTTATCTAAAT+TGG	-	chr4.3:34231525-34231544	MS.gene50738:intron	10.0%
!!	AGATATACTATTAATTACAA+AGG	+	chr4.3:34231157-34231176	None:intergenic	15.0%
!!	ATCTTTACAGAATTATTTAT+TGG	-	chr4.3:34231459-34231478	MS.gene50738:intron	15.0%
!!	GATATACTATTAATTACAAA+GGG	+	chr4.3:34231156-34231175	None:intergenic	15.0%
!!	TCTTTACAGAATTATTTATT+GGG	-	chr4.3:34231460-34231479	MS.gene50738:intron	15.0%
!!	AACCTATTTAAACATAAGTT+TGG	-	chr4.3:34230605-34230624	MS.gene50738:CDS	20.0%
!!	AATCTCCACTATAATAATAA+TGG	+	chr4.3:34230350-34230369	None:intergenic	20.0%
!!	AATTAATAGTATATCTGCTA+AGG	-	chr4.3:34231161-34231180	MS.gene50738:intron	20.0%
!!	ACCTATTTAAACATAAGTTT+GGG	-	chr4.3:34230606-34230625	MS.gene50738:CDS	20.0%
!!	ATACAATATCTAATAGAGTT+TGG	+	chr4.3:34231294-34231313	None:intergenic	20.0%
!!	ATATGTTATCTAAATTGGTT+TGG	-	chr4.3:34231530-34231549	MS.gene50738:intron	20.0%
!!	ATCTCCACTATAATAATAAT+GGG	+	chr4.3:34230349-34230368	None:intergenic	20.0%
!!	ATTAATTACAAAGGGAAAAT+AGG	+	chr4.3:34231148-34231167	None:intergenic	20.0%
!!	ATTGGATAATTAAAAACTCA+TGG	-	chr4.3:34232071-34232090	MS.gene50738:CDS	20.0%
!!	TATCTGATATTAATCTTTCT+AGG	-	chr4.3:34231238-34231257	MS.gene50738:intron	20.0%
!!	TATTCCCATTATTATTATAG+TGG	-	chr4.3:34230342-34230361	MS.gene50738:intron	20.0%
!!	TTGGATAATTAAAAACTCAT+GGG	-	chr4.3:34232072-34232091	MS.gene50738:CDS	20.0%
!!!	AAAACAAATTTTGATTGTGT+TGG	-	chr4.3:34230968-34230987	MS.gene50738:CDS	20.0%
!!!	AAACAAATTTTGATTGTGTT+GGG	-	chr4.3:34230969-34230988	MS.gene50738:CDS	20.0%
!!!	AAATGCTAACTTGTAATATT+TGG	-	chr4.3:34231483-34231502	MS.gene50738:intron	20.0%
!!!	AATGCTAACTTGTAATATTT+GGG	-	chr4.3:34231484-34231503	MS.gene50738:intron	20.0%
!!!	GAGTTTTTAATTATCCAATA+TGG	+	chr4.3:34232070-34232089	None:intergenic	20.0%
!!!	TTTGAGTACATTTTAAAATC+CGG	-	chr4.3:34231639-34231658	MS.gene50738:intron	20.0%
!	AAATGTTTAAGAACAGACAA+TGG	-	chr4.3:34228146-34228165	MS.gene50738:CDS	25.0%
!	ACTCTATTAGATATTGTATG+AGG	-	chr4.3:34231295-34231314	MS.gene50738:intron	25.0%
!	AGACAACCAATAAAGAAAAA+TGG	-	chr4.3:34228951-34228970	MS.gene50738:intron	25.0%
!	ATTTAAACATAAGTTTGGGA+AGG	-	chr4.3:34230610-34230629	MS.gene50738:CDS	25.0%
!	GTATATATGCAAACATACAT+TGG	-	chr4.3:34231941-34231960	MS.gene50738:intron	25.0%
!	TCCCAAACTTATGTTTAAAT+AGG	+	chr4.3:34230610-34230629	None:intergenic	25.0%
!	TGGATAATTAAAAACTCATG+GGG	-	chr4.3:34232073-34232092	MS.gene50738:CDS	25.0%
!	TTGCATATATACTGCATTTA+AGG	+	chr4.3:34231933-34231952	None:intergenic	25.0%
!!	AAAACTTCCCAAATCATTTT+GGG	-	chr4.3:34228319-34228338	MS.gene50738:intron	25.0%
!!	AGCAAATTTGAAACATGATT+AGG	-	chr4.3:34231391-34231410	MS.gene50738:intron	25.0%
!!	ATGCTAACTTGTAATATTTG+GGG	-	chr4.3:34231485-34231504	MS.gene50738:intron	25.0%
!!	TATTATAGTGGAGATTTAAG+AGG	-	chr4.3:34230354-34230373	MS.gene50738:intron	25.0%
!!	TATTTTTCCTTTTTGCCTTT+CGG	+	chr4.3:34229898-34229917	None:intergenic	25.0%
!!!	TTATCTAAATTGGTTTGGAT+TGG	-	chr4.3:34231535-34231554	MS.gene50738:intron	25.0%
!!!	TTTTGAAGAAATATTCTCAC+CGG	-	chr4.3:34229165-34229184	MS.gene50738:CDS	25.0%
	AAAATTACCAGATGGAAAGA+GGG	-	chr4.3:34229033-34229052	MS.gene50738:intron	30.0%
	AATCATCTTCCAAACATCAA+GGG	-	chr4.3:34227840-34227859	MS.gene50738:CDS	30.0%
	ACTATTGTGGTAAACAATTC+AGG	-	chr4.3:34228668-34228687	MS.gene50738:intron	30.0%
	AGTTACTGCTGTTAAAAATC+AGG	-	chr4.3:34230886-34230905	MS.gene50738:CDS	30.0%
	ATCTCAAGAAAGTTCTTGAA+AGG	-	chr4.3:34229251-34229270	MS.gene50738:CDS	30.0%
	ATGGATACTACAAAATATGC+AGG	-	chr4.3:34232113-34232132	MS.gene50738:CDS	30.0%
	ATGTAAACTTCACTGAGAAA+GGG	-	chr4.3:34228900-34228919	MS.gene50738:intron	30.0%
	ATGTGAGTAAGTAAAAACCA+CGG	+	chr4.3:34230390-34230409	None:intergenic	30.0%
	ATGTTTGTTCTACATCTGAA+AGG	-	chr4.3:34227976-34227995	MS.gene50738:intron	30.0%
	CAAAATGCAAATTGCATTTG+CGG	-	chr4.3:34230025-34230044	MS.gene50738:intron	30.0%
	CCAATAAAGAAAAATGGTTG+CGG	-	chr4.3:34228957-34228976	MS.gene50738:intron	30.0%
	CTATTGTGGTAAACAATTCA+GGG	-	chr4.3:34228669-34228688	MS.gene50738:intron	30.0%
	TAAAATTACCAGATGGAAAG+AGG	-	chr4.3:34229032-34229051	MS.gene50738:intron	30.0%
	TAGATACAACTCTCACAAAT+TGG	+	chr4.3:34230278-34230297	None:intergenic	30.0%
	TATATGCAAACATACATTGG+AGG	-	chr4.3:34231944-34231963	MS.gene50738:intron	30.0%
	TATGGTCATTTGAGAAACTT+TGG	-	chr4.3:34228443-34228462	MS.gene50738:intron	30.0%
	TATGTAAACTTCACTGAGAA+AGG	-	chr4.3:34228899-34228918	MS.gene50738:intron	30.0%
	TATTCCATGAGATCTACAAT+AGG	+	chr4.3:34228198-34228217	None:intergenic	30.0%
	TATTGTGGTAAACAATTCAG+GGG	-	chr4.3:34228670-34228689	MS.gene50738:intron	30.0%
	TCACGATCATAATTCATTTG+AGG	+	chr4.3:34228704-34228723	None:intergenic	30.0%
	TGGATACTACAAAATATGCA+GGG	-	chr4.3:34232114-34232133	MS.gene50738:CDS	30.0%
	TTACTAAGTTTCATATGACC+AGG	+	chr4.3:34229316-34229335	None:intergenic	30.0%
	TTCATCATCATCACTGATAA+AGG	-	chr4.3:34229640-34229659	MS.gene50738:intron	30.0%
	TTGTTTACCACAATAGTCTT+TGG	+	chr4.3:34228665-34228684	None:intergenic	30.0%
	TTTACTTACTCACATTGAGA+AGG	-	chr4.3:34230395-34230414	MS.gene50738:intron	30.0%
!	AAACTTCCCAAATCATTTTG+GGG	-	chr4.3:34228320-34228339	MS.gene50738:intron	30.0%
!	ATGATGATGAATTTCGAACT+AGG	+	chr4.3:34229631-34229650	None:intergenic	30.0%
!	CAAAACTTCCCAAATCATTT+TGG	-	chr4.3:34228318-34228337	MS.gene50738:intron	30.0%
!	GCATTTTGTCTTTTCCTTTT+TGG	+	chr4.3:34230013-34230032	None:intergenic	30.0%
!	TTCTTTATTGGTTGTCTCTA+TGG	+	chr4.3:34228948-34228967	None:intergenic	30.0%
!!	ATGAACTTGGCTTTAGATTA+TGG	-	chr4.3:34228555-34228574	MS.gene50738:intron	30.0%
!!	CAGAGTATCTTTTCAAAGAT+CGG	-	chr4.3:34227908-34227927	MS.gene50738:CDS	30.0%
!!	TGAACTTGGCTTTAGATTAT+GGG	-	chr4.3:34228556-34228575	MS.gene50738:intron	30.0%
!!!	AGTACATTTTAAAATCCGGT+GGG	-	chr4.3:34231643-34231662	MS.gene50738:intron	30.0%
	AATGAATATCAAATGCTCGC+TGG	-	chr4.3:34229565-34229584	MS.gene50738:intron	35.0%
	ACAATTCGTGCTTTGTATCT+GGG	+	chr4.3:34229112-34229131	None:intergenic	35.0%
	ACTCAAAATAGGCACTTTAG+CGG	+	chr4.3:34230461-34230480	None:intergenic	35.0%
	ACTTGATCTTTCGTCTTCAA+TGG	+	chr4.3:34228068-34228087	None:intergenic	35.0%
	AGAAGATTCAATAATCCGAC+AGG	-	chr4.3:34230538-34230557	MS.gene50738:CDS	35.0%
	AGATTATCTGTCGGAAGAAT+CGG	+	chr4.3:34230833-34230852	None:intergenic	35.0%
	AGTCTCTTTGAGCATATGTA+TGG	+	chr4.3:34231980-34231999	None:intergenic	35.0%
	AGTTTCATATGACCAGGTAA+CGG	+	chr4.3:34229310-34229329	None:intergenic	35.0%
	ATACCAGATGAAGTATGGTA+TGG	-	chr4.3:34228407-34228426	MS.gene50738:intron	35.0%
	ATCTACAATAGGCTGCAAAT+GGG	+	chr4.3:34228187-34228206	None:intergenic	35.0%
	ATGCAGGGAAAGATAAACAA+GGG	-	chr4.3:34227726-34227745	MS.gene50738:CDS	35.0%
	ATTGTATGAGGTTCGACTTA+AGG	-	chr4.3:34231307-34231326	MS.gene50738:intron	35.0%
	ATTGTGGTAAACAATTCAGG+GGG	-	chr4.3:34228671-34228690	MS.gene50738:intron	35.0%
	ATTTGAGAAACTTTGGATGC+AGG	-	chr4.3:34228450-34228469	MS.gene50738:intron	35.0%
	CTACTGAGTAGTAGTATGAA+CGG	+	chr4.3:34232185-34232204	None:intergenic	35.0%
	GAACTAGGATATCTCACATA+TGG	+	chr4.3:34229616-34229635	None:intergenic	35.0%
	GAATACAAATCTTCACCATC+AGG	+	chr4.3:34230327-34230346	None:intergenic	35.0%
	GAATCATCTTCCAAACATCA+AGG	-	chr4.3:34227839-34227858	MS.gene50738:CDS	35.0%
	GGATACTACAAAATATGCAG+GGG	-	chr4.3:34232115-34232134	MS.gene50738:CDS	35.0%
	GGGTTTGTGATCATGAATTA+GGG	-	chr4.3:34230989-34231008	MS.gene50738:CDS	35.0%
	GGTACAAGTATAATTGTGTG+CGG	-	chr4.3:34229763-34229782	MS.gene50738:intron	35.0%
	GTCTCTTTGAGCATATGTAT+GGG	+	chr4.3:34231979-34231998	None:intergenic	35.0%
	GTTTCTCAAATGACCATATG+AGG	+	chr4.3:34228441-34228460	None:intergenic	35.0%
	TAAGAAGCCAAAGACTATTG+TGG	-	chr4.3:34228655-34228674	MS.gene50738:intron	35.0%
	TATGCAGGGAAAGATAAACA+AGG	-	chr4.3:34227725-34227744	MS.gene50738:CDS	35.0%
	TATGGAAAGAAAGCCTCATA+TGG	-	chr4.3:34228425-34228444	MS.gene50738:intron	35.0%
	TCAATTGATGCTTCTTAGCA+CGG	+	chr4.3:34230694-34230713	None:intergenic	35.0%
	TCTCCATTTAAAGGTCTTGA+AGG	+	chr4.3:34228386-34228405	None:intergenic	35.0%
	TCTCCTTCAAGACCTTTAAA+TGG	-	chr4.3:34228380-34228399	MS.gene50738:intron	35.0%
	TCTGGTATCTCTCCATTTAA+AGG	+	chr4.3:34228395-34228414	None:intergenic	35.0%
	TGACTTTCTCTACAATCGTT+CGG	+	chr4.3:34228280-34228299	None:intergenic	35.0%
	TGGGTTTGTGATCATGAATT+AGG	-	chr4.3:34230988-34231007	MS.gene50738:CDS	35.0%
	TGTAAACTTCACTGAGAAAG+GGG	-	chr4.3:34228901-34228920	MS.gene50738:intron	35.0%
	TGTGAGTAAGTAAAAACCAC+GGG	+	chr4.3:34230389-34230408	None:intergenic	35.0%
	TGTTTAAGAACAGACAATGG+TGG	-	chr4.3:34228149-34228168	MS.gene50738:CDS	35.0%
	TGTTTCAATTGTGCTGAAAC+TGG	+	chr4.3:34230108-34230127	None:intergenic	35.0%
	TTTCCATACCATACTTCATC+TGG	+	chr4.3:34228413-34228432	None:intergenic	35.0%
	TTTGAGAAACTTTGGATGCA+GGG	-	chr4.3:34228451-34228470	MS.gene50738:intron	35.0%
!	AAATGGATACTGAGGTATCT+AGG	-	chr4.3:34229511-34229530	MS.gene50738:intron	35.0%
!	AATCACTGCCTTTCTTTTGA+TGG	+	chr4.3:34229147-34229166	None:intergenic	35.0%
!	AGATGATGATGATGTTGAGA+TGG	-	chr4.3:34228808-34228827	MS.gene50738:CDS	35.0%
!	AGCTTTGCTCTTTCATCTTT+TGG	+	chr4.3:34228491-34228510	None:intergenic	35.0%
!	CTTCTTGCATTGCTTGATTT+TGG	+	chr4.3:34228772-34228791	None:intergenic	35.0%
!	GCCAAAACATAAGCACTTTT+TGG	+	chr4.3:34229545-34229564	None:intergenic	35.0%
!	GTTCTTTTACTGGGTTTGAT+AGG	+	chr4.3:34229477-34229496	None:intergenic	35.0%
!	TTCCATCTGGTAATTTTACC+AGG	+	chr4.3:34229030-34229049	None:intergenic	35.0%
!!	AAAGTATTGTGAACCACCAA+GGG	+	chr4.3:34228003-34228022	None:intergenic	35.0%
!!	AGAGGGCATTGAAAAATAAG+TGG	-	chr4.3:34229050-34229069	MS.gene50738:intron	35.0%
!!	CCGCAACCATTTTTCTTTAT+TGG	+	chr4.3:34228960-34228979	None:intergenic	35.0%
!!	GAGGGCATTGAAAAATAAGT+GGG	-	chr4.3:34229051-34229070	MS.gene50738:intron	35.0%
!!	TGCTAAGAAGCATCAATTGA+TGG	-	chr4.3:34230694-34230713	MS.gene50738:intron	35.0%
!!!	AAGTGCCTATTTTGAGTTCA+CGG	-	chr4.3:34230464-34230483	MS.gene50738:CDS	35.0%
!!!	GAGTACATTTTAAAATCCGG+TGG	-	chr4.3:34231642-34231661	MS.gene50738:intron	35.0%
!!!	GATTTTGATTGGAGAGAGAA+AGG	-	chr4.3:34230860-34230879	MS.gene50738:CDS	35.0%
!!!	GCCAAAAAGTGCTTATGTTT+TGG	-	chr4.3:34229541-34229560	MS.gene50738:intron	35.0%
!!!	GTACATTTTAAAATCCGGTG+GGG	-	chr4.3:34231644-34231663	MS.gene50738:intron	35.0%
!!!	TGATTTTGGTTTGTCTGATC+AGG	+	chr4.3:34228758-34228777	None:intergenic	35.0%
	AAAGCACGAATTGTCGTGAA+GGG	-	chr4.3:34229118-34229137	MS.gene50738:CDS	40.0%
	AAAGCTAGATGCTAAGACGA+AGG	-	chr4.3:34228505-34228524	MS.gene50738:intron	40.0%
	AACAGAACCCAATCAAATGG+AGG	-	chr4.3:34228730-34228749	MS.gene50738:intron	40.0%
	AAGACATGAGCAAAGAACTG+TGG	-	chr4.3:34227766-34227785	MS.gene50738:CDS	40.0%
	AATCGTTCGGTTCATCCTTT+CGG	+	chr4.3:34228267-34228286	None:intergenic	40.0%
	ACATAGTTATGTCCACCTGA+TGG	-	chr4.3:34230309-34230328	MS.gene50738:intron	40.0%
	ACCGTTCTTGACAAGATTAG+CGG	+	chr4.3:34231793-34231812	None:intergenic	40.0%
	AGAGAAAGAAGAGATGAGCA+AGG	-	chr4.3:34229360-34229379	MS.gene50738:intron	40.0%
	AGATTCAATAATCCGACAGG+TGG	-	chr4.3:34230541-34230560	MS.gene50738:CDS	40.0%
	AGATTGCATCTCTTCTTCCA+TGG	+	chr4.3:34228984-34229003	None:intergenic	40.0%
	AGCAATGCAAGAAGAACAGA+TGG	-	chr4.3:34228778-34228797	MS.gene50738:CDS	40.0%
	ATCGTTCGAGAAGAATGTTG+TGG	-	chr4.3:34229212-34229231	MS.gene50738:CDS	40.0%
	ATGAATGTCATCTTCACCAC+CGG	+	chr4.3:34229187-34229206	None:intergenic	40.0%
	ATTATCTGTCGGAAGAATCG+GGG	+	chr4.3:34230831-34230850	None:intergenic	40.0%
	ATTGAATCTTCTCCGTCATC+CGG	+	chr4.3:34230530-34230549	None:intergenic	40.0%
	ATTGCTCGTGGTAAAAACGA+AGG	-	chr4.3:34227690-34227709	MS.gene50738:CDS	40.0%
	CAAACCCAGTAAAAGAACAC+TGG	-	chr4.3:34229479-34229498	MS.gene50738:intron	40.0%
	CAACATGACAGCTTCCAAAA+AGG	-	chr4.3:34229996-34230015	MS.gene50738:intron	40.0%
	CAATTCGTGCTTTGTATCTG+GGG	+	chr4.3:34229111-34229130	None:intergenic	40.0%
	CATCTGAATGAACAAGATCG+AGG	+	chr4.3:34227959-34227978	None:intergenic	40.0%
	CCAAGGATGAACATGATTAC+AGG	-	chr4.3:34230642-34230661	MS.gene50738:CDS	40.0%
	CCTGTAATCATGTTCATCCT+TGG	+	chr4.3:34230645-34230664	None:intergenic	40.0%
	CTAAAGCCAAGTTCATCTCT+TGG	+	chr4.3:34228551-34228570	None:intergenic	40.0%
	CTATTCGCCTATTAGGATGA+AGG	-	chr4.3:34232039-34232058	MS.gene50738:CDS	40.0%
	CTCGTTGATCTTCTCAATTG+AGG	+	chr4.3:34228842-34228861	None:intergenic	40.0%
	CTTCATCCTAATAGGCGAAT+AGG	+	chr4.3:34232041-34232060	None:intergenic	40.0%
	CTTTAGCACTTACTAGCAAG+AGG	+	chr4.3:34231816-34231835	None:intergenic	40.0%
	GACAATTCGTGCTTTGTATC+TGG	+	chr4.3:34229113-34229132	None:intergenic	40.0%
	GACCTGGTAAAATTACCAGA+TGG	-	chr4.3:34229025-34229044	MS.gene50738:intron	40.0%
	GACGAAAGATCAAGTTCTTC+AGG	-	chr4.3:34228073-34228092	MS.gene50738:CDS	40.0%
	GAGAGATACCAGATGAAGTA+TGG	-	chr4.3:34228402-34228421	MS.gene50738:intron	40.0%
	GATCTACAATAGGCTGCAAA+TGG	+	chr4.3:34228188-34228207	None:intergenic	40.0%
	GATGAGTATGATGAATTGCG+AGG	-	chr4.3:34229808-34229827	MS.gene50738:intron	40.0%
	GATTATCTGTCGGAAGAATC+GGG	+	chr4.3:34230832-34230851	None:intergenic	40.0%
	GCTAACAGAACCCAATCAAA+TGG	-	chr4.3:34228727-34228746	MS.gene50738:intron	40.0%
	GCTTCACCCCAAAATGATTT+GGG	+	chr4.3:34228329-34228348	None:intergenic	40.0%
	GCTTCACGTGAAGTTTCATA+AGG	+	chr4.3:34229935-34229954	None:intergenic	40.0%
	GCTTCTTAGATTGAATGTCG+TGG	+	chr4.3:34228643-34228662	None:intergenic	40.0%
	GTGAAGTTTCATAAGGCAAG+TGG	+	chr4.3:34229928-34229947	None:intergenic	40.0%
	TACAAATCTTCACCATCAGG+TGG	+	chr4.3:34230324-34230343	None:intergenic	40.0%
	TCTCTCCAATCAAAATCCTC+CGG	+	chr4.3:34230857-34230876	None:intergenic	40.0%
	TCTTCCGACAGATAATCTTC+CGG	-	chr4.3:34230835-34230854	MS.gene50738:CDS	40.0%
	TCTTTCTCTTGGTCATTCTC+AGG	+	chr4.3:34229349-34229368	None:intergenic	40.0%
	TGAAGAAATATTCTCACCGG+TGG	-	chr4.3:34229168-34229187	MS.gene50738:CDS	40.0%
	TGCTCAAAGAGACTAGATCA+TGG	-	chr4.3:34231986-34232005	MS.gene50738:intron	40.0%
	TGCTCATCTCTTCTTTCTCT+TGG	+	chr4.3:34229360-34229379	None:intergenic	40.0%
	TGCTTCACCCCAAAATGATT+TGG	+	chr4.3:34228330-34228349	None:intergenic	40.0%
	TGGGAAGGTTTATTCTTCCA+AGG	-	chr4.3:34230625-34230644	MS.gene50738:CDS	40.0%
	TGTTGGAGTTTCAGTACAAC+CGG	-	chr4.3:34231025-34231044	MS.gene50738:CDS	40.0%
!	ACGATCACTCTAGAAAAGTG+TGG	-	chr4.3:34228036-34228055	MS.gene50738:intron	40.0%
!	ACTAGATCATGGAGTGTTAC+TGG	-	chr4.3:34231997-34232016	MS.gene50738:intron	40.0%
!	ACTTGTCGATTGTGATCATG+AGG	-	chr4.3:34231108-34231127	MS.gene50738:intron	40.0%
!	ATAGTTTTCCTTTGGCTGGA+GGG	-	chr4.3:34229831-34229850	MS.gene50738:intron	40.0%
!	ATTTTACCAGGTCATACGTC+TGG	+	chr4.3:34229018-34229037	None:intergenic	40.0%
!	CACTCCAGTGTTCTTTTACT+GGG	+	chr4.3:34229486-34229505	None:intergenic	40.0%
!	CGATCACTCTAGAAAAGTGT+GGG	-	chr4.3:34228037-34228056	MS.gene50738:intron	40.0%
!	GACAATGGTGGAGAGTATAT+AGG	-	chr4.3:34228161-34228180	MS.gene50738:CDS	40.0%
!	GCATCAATTGATGGATCCTT+CGG	-	chr4.3:34230703-34230722	MS.gene50738:intron	40.0%
!	GCTAAGACTGACAAGTTTTC+CGG	+	chr4.3:34231080-34231099	None:intergenic	40.0%
!	GTTGTTGTTGTTGTGCAAGT+TGG	+	chr4.3:34229980-34229999	None:intergenic	40.0%
!	TCTAGAGTGATCGTCAATGA+AGG	+	chr4.3:34228030-34228049	None:intergenic	40.0%
!	TTGCGAGGATAGTTTTCCTT+TGG	-	chr4.3:34229823-34229842	MS.gene50738:intron	40.0%
!	TTTTCTCTCACACTCTTACG+AGG	+	chr4.3:34230245-34230264	None:intergenic	40.0%
!!	ATCTTCCGGAGGATTTTGAT+TGG	-	chr4.3:34230849-34230868	MS.gene50738:CDS	40.0%
!!	CAAAGTATTGTGAACCACCA+AGG	+	chr4.3:34228004-34228023	None:intergenic	40.0%
!!!	CACACAACAAGTTTTTAGCG+TGG	+	chr4.3:34230425-34230444	None:intergenic	40.0%
!!!	TATTTTGAGTTCACGGAGGA+GGG	-	chr4.3:34230471-34230490	MS.gene50738:CDS	40.0%
	AAAACTCATGGGGTGAGACT+TGG	-	chr4.3:34232083-34232102	MS.gene50738:CDS	45.0%
	AAACATCAAGGGTCAACCAC+TGG	-	chr4.3:34227851-34227870	MS.gene50738:CDS	45.0%
	AAACTCATGGGGTGAGACTT+GGG	-	chr4.3:34232084-34232103	MS.gene50738:CDS	45.0%
	AAACTCCCTCGGAGTAAGAT+CGG	+	chr4.3:34230753-34230772	None:intergenic	45.0%
	AACTTCACGTGAAGCAGCAA+AGG	-	chr4.3:34229940-34229959	MS.gene50738:intron	45.0%
	AAGCACGAATTGTCGTGAAG+GGG	-	chr4.3:34229119-34229138	MS.gene50738:CDS	45.0%
	AATTCCATGCCTCCGTAGAA+CGG	-	chr4.3:34228105-34228124	MS.gene50738:CDS	45.0%
	AATTCTCCGATCTTACTCCG+AGG	-	chr4.3:34230744-34230763	MS.gene50738:intron	45.0%
	AGAAAAATGGTTGCGGTCCA+TGG	-	chr4.3:34228964-34228983	MS.gene50738:intron	45.0%
	AGATGAATGGAGTAGCCGAA+AGG	-	chr4.3:34228249-34228268	MS.gene50738:CDS	45.0%
	AGGCTCTCTGTATGTTATGC+AGG	-	chr4.3:34227710-34227729	MS.gene50738:CDS	45.0%
	AGTGGTTGACCCTTGATGTT+TGG	+	chr4.3:34227852-34227871	None:intergenic	45.0%
	ATAGTGTGATCCAGAGCAAC+CGG	-	chr4.3:34231572-34231591	MS.gene50738:intron	45.0%
	ATCAGGCTCCTCCATTTGAT+TGG	+	chr4.3:34228741-34228760	None:intergenic	45.0%
	ATCATGAATTAGGGCTCGTG+TGG	-	chr4.3:34230998-34231017	MS.gene50738:CDS	45.0%
	ATGAAAGAGCTTCCGCTTGA+GGG	-	chr4.3:34229786-34229805	MS.gene50738:intron	45.0%
	ATGGCTTCCATGAAGCTTTG+AGG	+	chr4.3:34228929-34228948	None:intergenic	45.0%
	ATTCTCCGATCTTACTCCGA+GGG	-	chr4.3:34230745-34230764	MS.gene50738:intron	45.0%
	CAAAGCACGAATTGTCGTGA+AGG	-	chr4.3:34229117-34229136	MS.gene50738:CDS	45.0%
	CAATCTTCACAGGATTCCAG+TGG	+	chr4.3:34227870-34227889	None:intergenic	45.0%
	CATCATACTCATCCCTCAAG+CGG	+	chr4.3:34229801-34229820	None:intergenic	45.0%
	CCACATGAGCGAGAAAAGTT+TGG	-	chr4.3:34227803-34227822	MS.gene50738:CDS	45.0%
	CCACGATCAGTACCAGAATA+AGG	+	chr4.3:34231690-34231709	None:intergenic	45.0%
	CCATCAAGCAGATACTTCTC+AGG	-	chr4.3:34228872-34228891	MS.gene50738:intron	45.0%
	CCATTCATCTGAGGAGTCTT+GGG	+	chr4.3:34228239-34228258	None:intergenic	45.0%
	CGAACCATCTTCTTAGTAGC+AGG	+	chr4.3:34228584-34228603	None:intergenic	45.0%
	CTACCCACAGCTGAAGAATA+GGG	+	chr4.3:34229388-34229407	None:intergenic	45.0%
	CTCCTCCGTGAACTCAAAAT+AGG	+	chr4.3:34230472-34230491	None:intergenic	45.0%
	GCAGCCTATTGTAGATCTCA+TGG	-	chr4.3:34228191-34228210	MS.gene50738:CDS	45.0%
	GCCGCTAATCTTGTCAAGAA+CGG	-	chr4.3:34231789-34231808	MS.gene50738:intron	45.0%
	GGATCCTGCTACTAAGAAGA+TGG	-	chr4.3:34228577-34228596	MS.gene50738:intron	45.0%
	GGATGAAGGAGAAGCCATAT+TGG	-	chr4.3:34232053-34232072	MS.gene50738:CDS	45.0%
	GGCTCTCTGTATGTTATGCA+GGG	-	chr4.3:34227711-34227730	MS.gene50738:CDS	45.0%
	GGCTTCTCCTTCATCCTAAT+AGG	+	chr4.3:34232049-34232068	None:intergenic	45.0%
	GGTTCACCAAGAGATGAACT+TGG	-	chr4.3:34228542-34228561	MS.gene50738:intron	45.0%
	GTGCTGTGAAATGGATACTG+AGG	-	chr4.3:34229503-34229522	MS.gene50738:intron	45.0%
	GTGGAGATTTAAGAGGCCTA+CGG	-	chr4.3:34230361-34230380	MS.gene50738:intron	45.0%
	GTTCATCTCTTGGTGAACCA+AGG	+	chr4.3:34228541-34228560	None:intergenic	45.0%
	TCAAATGCTCGCTGGATATG+TGG	-	chr4.3:34229573-34229592	MS.gene50738:intron	45.0%
	TCACTGATAAAGGAGAGTCC+TGG	-	chr4.3:34229650-34229669	MS.gene50738:intron	45.0%
	TCAGGCTCCTCCATTTGATT+GGG	+	chr4.3:34228740-34228759	None:intergenic	45.0%
	TCCATTCATCTGAGGAGTCT+TGG	+	chr4.3:34228240-34228259	None:intergenic	45.0%
	TCCTCCGGAAGATTATCTGT+CGG	+	chr4.3:34230842-34230861	None:intergenic	45.0%
	TCCTGTGAAGATTGTCTAGC+AGG	-	chr4.3:34227876-34227895	MS.gene50738:CDS	45.0%
	TCGAAAGTCCTCCTGACTAA+GGG	-	chr4.3:34229703-34229722	MS.gene50738:intron	45.0%
	TCTACATCTGAAAGGTCCCT+TGG	-	chr4.3:34227984-34228003	MS.gene50738:intron	45.0%
	TCTCACATCTCTGTGTGTTG+TGG	+	chr4.3:34230131-34230150	None:intergenic	45.0%
	TCTTCCAGTTGTCCGTTCTA+CGG	+	chr4.3:34228120-34228139	None:intergenic	45.0%
	TCTTCTCACAACTCTGTGTG+AGG	+	chr4.3:34230201-34230220	None:intergenic	45.0%
	TGACAAGATTAGCGGCAATC+TGG	+	chr4.3:34231785-34231804	None:intergenic	45.0%
	TGCAGGGGACGAAATATATG+CGG	-	chr4.3:34232130-34232149	MS.gene50738:CDS	45.0%
	TGGAGATTTAAGAGGCCTAC+GGG	-	chr4.3:34230362-34230381	MS.gene50738:intron	45.0%
	TTACTGGTGGGATATGGATC+TGG	-	chr4.3:34232013-34232032	MS.gene50738:intron	45.0%
	TTCAATGCCCTCTTTCCATC+TGG	+	chr4.3:34229043-34229062	None:intergenic	45.0%
	TTTCCCAACATTCTCTACGC+CGG	-	chr4.3:34230508-34230527	MS.gene50738:CDS	45.0%
!	ACGAAGGAGTGCATATACCT+TGG	-	chr4.3:34228521-34228540	MS.gene50738:intron	45.0%
!	AGATCATGGAGTGTTACTGG+TGG	-	chr4.3:34232000-34232019	MS.gene50738:intron	45.0%
!	CCAAACTTTTCTCGCTCATG+TGG	+	chr4.3:34227806-34227825	None:intergenic	45.0%
!	GAGGATAGTTTTCCTTTGGC+TGG	-	chr4.3:34229827-34229846	MS.gene50738:intron	45.0%
!	GATAGTTTTCCTTTGGCTGG+AGG	-	chr4.3:34229830-34229849	MS.gene50738:intron	45.0%
!	GATCATGGAGTGTTACTGGT+GGG	-	chr4.3:34232001-34232020	MS.gene50738:intron	45.0%
!	GCACATCGATAGTGACTTGT+AGG	-	chr4.3:34231343-34231362	MS.gene50738:intron	45.0%
!	GCACTCCAGTGTTCTTTTAC+TGG	+	chr4.3:34229487-34229506	None:intergenic	45.0%
!	GGAACTTTTCTCCACATGGT+GGG	+	chr4.3:34229873-34229892	None:intergenic	45.0%
!	GTGCTTTGTATCTGGGGTTT+GGG	+	chr4.3:34229105-34229124	None:intergenic	45.0%
!	TAGTTTTCCTTTGGCTGGAG+GGG	-	chr4.3:34229832-34229851	MS.gene50738:intron	45.0%
!	TCAATTGATGGATCCTTCGG+CGG	-	chr4.3:34230706-34230725	MS.gene50738:intron	45.0%
!	TGGAACTTTTCTCCACATGG+TGG	+	chr4.3:34229874-34229893	None:intergenic	45.0%
!	TGGATACTGAGGTATCTAGG+AGG	-	chr4.3:34229514-34229533	MS.gene50738:intron	45.0%
!	TTTCCTTTTTGCCTTTCGGC+TGG	+	chr4.3:34229894-34229913	None:intergenic	45.0%
!!	AACACTGGAGTGCTGTGAAA+TGG	-	chr4.3:34229494-34229513	MS.gene50738:intron	45.0%
!!	AAGGGGTGCCATCAAAAGAA+AGG	-	chr4.3:34229136-34229155	MS.gene50738:CDS	45.0%
!!	CCTGAGAAGTATCTGCTTGA+TGG	+	chr4.3:34228875-34228894	None:intergenic	45.0%
!!	CCTTATTCTGGTACTGATCG+TGG	-	chr4.3:34231687-34231706	MS.gene50738:intron	45.0%
!!	GAGTTTCGTCAGAGCGTTTT+GGG	-	chr4.3:34230767-34230786	MS.gene50738:intron	45.0%
!!!	AGAGCGTTTTGGGATTGAGA+GGG	-	chr4.3:34230777-34230796	MS.gene50738:intron	45.0%
!!!	CTATTTTGAGTTCACGGAGG+AGG	-	chr4.3:34230470-34230489	MS.gene50738:CDS	45.0%
!!!	TGCCTATTTTGAGTTCACGG+AGG	-	chr4.3:34230467-34230486	MS.gene50738:CDS	45.0%
	AACTCATGGGGTGAGACTTG+GGG	-	chr4.3:34232085-34232104	MS.gene50738:CDS	50.0%
	ACATCTGAAAGGTCCCTTGG+TGG	-	chr4.3:34227987-34228006	MS.gene50738:intron	50.0%
	ACATTCTCTACGCCGGATGA+CGG	-	chr4.3:34230515-34230534	MS.gene50738:CDS	50.0%
	ACGGAGGAGGGAAATTATGC+AGG	-	chr4.3:34230483-34230502	MS.gene50738:CDS	50.0%
	AGAGAACCAGACGTATGACC+TGG	-	chr4.3:34229009-34229028	MS.gene50738:CDS	50.0%
	AGGGGAACCTCAAAGCTTCA+TGG	-	chr4.3:34228919-34228938	MS.gene50738:intron	50.0%
	AGTACAACCGGTGCACTTGA+AGG	-	chr4.3:34231037-34231056	MS.gene50738:intron	50.0%
	CAACTCTCTTCGTGAGAGTG+AGG	+	chr4.3:34230166-34230185	None:intergenic	50.0%
	CATCCGGCGTAGAGAATGTT+GGG	+	chr4.3:34230514-34230533	None:intergenic	50.0%
	CCCAAGACTCCTCAGATGAA+TGG	-	chr4.3:34228236-34228255	MS.gene50738:CDS	50.0%
	CCTACAGCATAACCGATGTC+TGG	+	chr4.3:34229439-34229458	None:intergenic	50.0%
	CTGGAGCCTATTCGCCTATT+AGG	-	chr4.3:34232032-34232051	MS.gene50738:CDS	50.0%
	GAACTGTGGCACAAACGACT+TGG	-	chr4.3:34227780-34227799	MS.gene50738:CDS	50.0%
	GATGAAAGAGCTTCCGCTTG+AGG	-	chr4.3:34229785-34229804	MS.gene50738:intron	50.0%
	GCCTGCTAGACAATCTTCAC+AGG	+	chr4.3:34227880-34227899	None:intergenic	50.0%
	GCTACCCACAGCTGAAGAAT+AGG	+	chr4.3:34229389-34229408	None:intergenic	50.0%
	GGACTCCATGGTTTCTACTG+TGG	-	chr4.3:34232156-34232175	MS.gene50738:CDS	50.0%
	GGAGAAAAGTTCCAGCCGAA+AGG	-	chr4.3:34229880-34229899	MS.gene50738:intron	50.0%
	GGGACGAAATATATGCGGAG+TGG	-	chr4.3:34232135-34232154	MS.gene50738:CDS	50.0%
	GGGGCTGCATTTGCATCTTT+CGG	+	chr4.3:34230812-34230831	None:intergenic	50.0%
	GGTGAGACTTGGGGAGAAAA+TGG	-	chr4.3:34232094-34232113	MS.gene50738:CDS	50.0%
	GGTTCCGTTGAATGCGACAA+CGG	-	chr4.3:34229739-34229758	MS.gene50738:intron	50.0%
	GGTTTGACGAAAGGTTCGAC+CGG	-	chr4.3:34229671-34229690	MS.gene50738:intron	50.0%
	GTCGAAAGTCCTCCTGACTA+AGG	-	chr4.3:34229702-34229721	MS.gene50738:intron	50.0%
	GTCGAACCTTTCGTCAAACC+AGG	+	chr4.3:34229671-34229690	None:intergenic	50.0%
	GTGCCCTATTCTTCAGCTGT+GGG	-	chr4.3:34229382-34229401	MS.gene50738:intron	50.0%
	GTTCCAGCCGAAAGGCAAAA+AGG	-	chr4.3:34229888-34229907	MS.gene50738:intron	50.0%
	TAGTGTGATCCAGAGCAACC+GGG	-	chr4.3:34231573-34231592	MS.gene50738:intron	50.0%
	TCAGGAGGACTTTCGACACA+AGG	+	chr4.3:34229699-34229718	None:intergenic	50.0%
	TCATCCGGCGTAGAGAATGT+TGG	+	chr4.3:34230515-34230534	None:intergenic	50.0%
	TCCGACAGATAATCTTCCGG+AGG	-	chr4.3:34230838-34230857	MS.gene50738:CDS	50.0%
	TCGGCTACTCCATTCATCTG+AGG	+	chr4.3:34228248-34228267	None:intergenic	50.0%
	TGCAGACCATGGCGTACATA+AGG	+	chr4.3:34229411-34229430	None:intergenic	50.0%
	TGGTCGACGATGAAGAAGTG+CGG	-	chr4.3:34230561-34230580	MS.gene50738:CDS	50.0%
	TTCATCTGAGGAGTCTTGGG+AGG	+	chr4.3:34228236-34228255	None:intergenic	50.0%
!	AGTTTTCCTTTGGCTGGAGG+GGG	-	chr4.3:34229833-34229852	MS.gene50738:intron	50.0%
!	CCAGACATCGGTTATGCTGT+AGG	-	chr4.3:34229436-34229455	MS.gene50738:intron	50.0%
!	CGTGCTTTGTATCTGGGGTT+TGG	+	chr4.3:34229106-34229125	None:intergenic	50.0%
!	GACGATGAAGAAGTGCGGTT+GGG	-	chr4.3:34230566-34230585	MS.gene50738:CDS	50.0%
!	GAGAGTCCTGGTTTGACGAA+AGG	-	chr4.3:34229662-34229681	MS.gene50738:intron	50.0%
!	GGAGTGTTACTGGTGGGATA+TGG	-	chr4.3:34232007-34232026	MS.gene50738:intron	50.0%
!	GGCTGGAACTTTTCTCCACA+TGG	+	chr4.3:34229877-34229896	None:intergenic	50.0%
!	TATATGCGGAGTGGACTCCA+TGG	-	chr4.3:34232144-34232163	MS.gene50738:CDS	50.0%
!!	GGAGTTTCGTCAGAGCGTTT+TGG	-	chr4.3:34230766-34230785	MS.gene50738:intron	50.0%
!!!	CAGAGCGTTTTGGGATTGAG+AGG	-	chr4.3:34230776-34230795	MS.gene50738:intron	50.0%
!!!	GAGCGTTTTGGGATTGAGAG+GGG	-	chr4.3:34230778-34230797	MS.gene50738:intron	50.0%
!!!	GTTTTGGGATTGAGAGGGGT+GGG	-	chr4.3:34230782-34230801	MS.gene50738:intron	50.0%
!!!	TTTTGGGATTGAGAGGGGTG+GGG	-	chr4.3:34230783-34230802	MS.gene50738:intron	50.0%
	AACCGGGTTCTTGTGATGCC+GGG	-	chr4.3:34231589-34231608	MS.gene50738:intron	55.0%
	AATTCTCCCCCTCCAGCCAA+AGG	+	chr4.3:34229842-34229861	None:intergenic	55.0%
	ACCGCCGTTGTCGCATTCAA+CGG	+	chr4.3:34229746-34229765	None:intergenic	55.0%
	ACGCTCTGACGAAACTCCCT+CGG	+	chr4.3:34230764-34230783	None:intergenic	55.0%
	AGGGCTCGTGTGGATCATGT+TGG	-	chr4.3:34231008-34231027	MS.gene50738:CDS	55.0%
	ATGGATCCTTCGGCGGTTCA+CGG	-	chr4.3:34230713-34230732	MS.gene50738:intron	55.0%
	ATTGTTGGGGTCCCACCATG+TGG	-	chr4.3:34229859-34229878	MS.gene50738:intron	55.0%
	CAACCGGGTTCTTGTGATGC+CGG	-	chr4.3:34231588-34231607	MS.gene50738:intron	55.0%
	CATCAGACCACCATTGCACC+CGG	+	chr4.3:34231610-34231629	None:intergenic	55.0%
	CCTGAACACCCTTAGTCAGG+AGG	+	chr4.3:34229714-34229733	None:intergenic	55.0%
	CCTGTGCATGTTCCGTTACC+TGG	-	chr4.3:34229295-34229314	MS.gene50738:CDS	55.0%
	CTGGAGGGGGAGAATTGTTG+GGG	-	chr4.3:34229846-34229865	MS.gene50738:intron	55.0%
	CTTCATCGTCGACCACCTGT+CGG	+	chr4.3:34230556-34230575	None:intergenic	55.0%
	GATGGTTCGAAGCAGAGACG+TGG	-	chr4.3:34228595-34228614	MS.gene50738:intron	55.0%
	GCTGGAGGGGGAGAATTGTT+GGG	-	chr4.3:34229845-34229864	MS.gene50738:intron	55.0%
	GGACTTTCGACACAAGGAGC+CGG	+	chr4.3:34229693-34229712	None:intergenic	55.0%
	GGCAGCCACAGTAGAAACCA+TGG	+	chr4.3:34232164-34232183	None:intergenic	55.0%
	GGTGCCCTATTCTTCAGCTG+TGG	-	chr4.3:34229381-34229400	MS.gene50738:intron	55.0%
	GGTGCTCACTTTCTCTCGAC+CGG	-	chr4.3:34231058-34231077	MS.gene50738:intron	55.0%
	GTAAAAACCACGGGACCCGT+AGG	+	chr4.3:34230380-34230399	None:intergenic	55.0%
	GTGAGCACCTTCAAGTGCAC+CGG	+	chr4.3:34231047-34231066	None:intergenic	55.0%
	TCACAAGAACCCGGTTGCTC+TGG	+	chr4.3:34231585-34231604	None:intergenic	55.0%
	TCCAGTTGTCCGTTCTACGG+AGG	+	chr4.3:34228117-34228136	None:intergenic	55.0%
	TCCGTTGAATGCGACAACGG+CGG	-	chr4.3:34229742-34229761	MS.gene50738:intron	55.0%
	TCTTGTGATGCCGGGTGCAA+TGG	-	chr4.3:34231597-34231616	MS.gene50738:intron	55.0%
	TTGTCCGTTCTACGGAGGCA+TGG	+	chr4.3:34228112-34228131	None:intergenic	55.0%
!	CGACGATGAAGAAGTGCGGT+TGG	-	chr4.3:34230565-34230584	MS.gene50738:CDS	55.0%
!!	CCAGGTAACGGAACATGCAC+AGG	+	chr4.3:34229298-34229317	None:intergenic	55.0%
!!	CCTCCTGACTAAGGGTGTTC+AGG	-	chr4.3:34229711-34229730	MS.gene50738:intron	55.0%
!!	CCTGACTAAGGGTGTTCAGG+CGG	-	chr4.3:34229714-34229733	MS.gene50738:intron	55.0%
!!	GGGTAGCCTTATGTACGCCA+TGG	-	chr4.3:34229402-34229421	MS.gene50738:intron	55.0%
!!!	CGTTTTGGGATTGAGAGGGG+TGG	-	chr4.3:34230781-34230800	MS.gene50738:intron	55.0%
	CACCCGGCATCACAAGAACC+CGG	+	chr4.3:34231594-34231613	None:intergenic	60.0%
	CCGCCTGAACACCCTTAGTC+AGG	+	chr4.3:34229717-34229736	None:intergenic	60.0%
	GCCTCCGTAGAACGGACAAC+TGG	-	chr4.3:34228113-34228132	MS.gene50738:CDS	60.0%
	GGCTGGAGGGGGAGAATTGT+TGG	-	chr4.3:34229844-34229863	MS.gene50738:intron	60.0%
	GTAACACCGTGAACCGCCGA+AGG	+	chr4.3:34230722-34230741	None:intergenic	60.0%
	GTCTGCACGAGGCCAGACAT+CGG	-	chr4.3:34229424-34229443	MS.gene50738:intron	60.0%
	TGTACGCCATGGTCTGCACG+AGG	-	chr4.3:34229413-34229432	MS.gene50738:intron	60.0%
	TGTGATGCCGGGTGCAATGG+TGG	-	chr4.3:34231600-34231619	MS.gene50738:intron	60.0%
	TTCTTCGCGCATCACCCCAC+CGG	+	chr4.3:34231661-34231680	None:intergenic	60.0%
!!	ACTAAGGGTGTTCAGGCGGC+GGG	-	chr4.3:34229718-34229737	MS.gene50738:intron	60.0%
!!	GACTAAGGGTGTTCAGGCGG+CGG	-	chr4.3:34229717-34229736	MS.gene50738:intron	60.0%
	GTCTGGCCTCGTGCAGACCA+TGG	+	chr4.3:34229422-34229441	None:intergenic	65.0%
	TAAGAGGCCTACGGGTCCCG+TGG	-	chr4.3:34230370-34230389	MS.gene50738:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	34227684	34232204	34227684	ID=MS.gene50738
chr4.3	mRNA	34227684	34232204	34227684	ID=MS.gene50738.t1;Parent=MS.gene50738
chr4.3	exon	34232027	34232204	34232027	ID=MS.gene50738.t1.exon1;Parent=MS.gene50738.t1
chr4.3	CDS	34232027	34232204	34232027	ID=cds.MS.gene50738.t1;Parent=MS.gene50738.t1
chr4.3	exon	34230820	34231051	34230820	ID=MS.gene50738.t1.exon2;Parent=MS.gene50738.t1
chr4.3	CDS	34230820	34231051	34230820	ID=cds.MS.gene50738.t1;Parent=MS.gene50738.t1
chr4.3	exon	34230426	34230678	34230426	ID=MS.gene50738.t1.exon3;Parent=MS.gene50738.t1
chr4.3	CDS	34230426	34230678	34230426	ID=cds.MS.gene50738.t1;Parent=MS.gene50738.t1
chr4.3	exon	34229089	34229328	34229089	ID=MS.gene50738.t1.exon4;Parent=MS.gene50738.t1
chr4.3	CDS	34229089	34229328	34229089	ID=cds.MS.gene50738.t1;Parent=MS.gene50738.t1
chr4.3	exon	34228981	34229028	34228981	ID=MS.gene50738.t1.exon5;Parent=MS.gene50738.t1
chr4.3	CDS	34228981	34229028	34228981	ID=cds.MS.gene50738.t1;Parent=MS.gene50738.t1
chr4.3	exon	34228759	34228878	34228759	ID=MS.gene50738.t1.exon6;Parent=MS.gene50738.t1
chr4.3	CDS	34228759	34228878	34228759	ID=cds.MS.gene50738.t1;Parent=MS.gene50738.t1
chr4.3	exon	34228066	34228312	34228066	ID=MS.gene50738.t1.exon7;Parent=MS.gene50738.t1
chr4.3	CDS	34228066	34228312	34228066	ID=cds.MS.gene50738.t1;Parent=MS.gene50738.t1
chr4.3	exon	34227684	34227961	34227684	ID=MS.gene50738.t1.exon8;Parent=MS.gene50738.t1
chr4.3	CDS	34227684	34227961	34227684	ID=cds.MS.gene50738.t1;Parent=MS.gene50738.t1

Gene Sequence

>MS.gene50738

ATGATCATTGCTCGTGGTAAAAACGAAGGCTCTCTGTATGTTATGCAGGGAAAGATAAACAAGGGAGATATCAATGTATCTCAAGACATGAGCAAAGAACTGTGGCACAAACGACTTGGCCACATGAGCGAGAAAAGTTTGGAGATTCTTGCTAAGAATCATCTTCCAAACATCAAGGGTCAACCACTGGAATCCTGTGAAGATTGTCTAGCAGGCAAACAACACAGAGTATCTTTTCAAAGATCGGATGATGCAAGAAGAAGAAAGCATATCCTCGATCTTGTTCATTCAGATGTTTGTTCTACATCTGAAAGGTCCCTTGGTGGTTCACAATACTTTGTTACCTTCATTGACGATCACTCTAGAAAAGTGTGGGTGTATCCATTGAAGACGAAAGATCAAGTTCTTCAGGTTTTCAAAGAATTCCATGCCTCCGTAGAACGGACAACTGGAAGAAAACTGAAATGTTTAAGAACAGACAATGGTGGAGAGTATATAGGCCCATTTGCAGCCTATTGTAGATCTCATGGAATAAGACATGAGAAAGTACCTCCCAAGACTCCTCAGATGAATGGAGTAGCCGAAAGGATGAACCGAACGATTGTAGAGAAAGTCAGAAGTATACTTTCTCAAGCAAAACTTCCCAAATCATTTTGGGGTGAAGCAGTGAGAACTGCAGTTGATTTGATAAATTTGTCTCCTTCAAGACCTTTAAATGGAGAGATACCAGATGAAGTATGGTATGGAAAGAAAGCCTCATATGGTCATTTGAGAAACTTTGGATGCAGGGCATTTGTGCATGTTCCAAAAGATGAAAGAGCAAAGCTAGATGCTAAGACGAAGGAGTGCATATACCTTGGTTCACCAAGAGATGAACTTGGCTTTAGATTATGGGATCCTGCTACTAAGAAGATGGTTCGAAGCAGAGACGTGGTATTCTATGAAGATCAGACAATCCACGACATTCAATCTAAGAAGCCAAAGACTATTGTGGTAAACAATTCAGGGGGAGAGACTCCTCAAATGAATTATGATCGTGATCAGCTAACAGAACCCAATCAAATGGAGGAGCCTGATCAGACAAACCAAAATCAAGCAATGCAAGAAGAACAGATGGCAGATGCAGATGATGATGATGTTGAGATGGAACAAGAACCTCAATTGAGAAGATCAACGAGATCTAGACAACCATCAAGCAGATACTTCTCAGGTGAATATGTAAACTTCACTGAGAAAGGGGAACCTCAAAGCTTCATGGAAGCCATAGAGACAACCAATAAAGAAAAATGGTTGCGGTCCATGGAAGAAGAGATGCAATCTCTAAAAGAGAACCAGACGTATGACCTGGTAAAATTACCAGATGGAAAGAGGGCATTGAAAAATAAGTGGGTGTTCAAGCTCAAGTCTGAAGAGAACAACCCAAACCCCAGATACAAAGCACGAATTGTCGTGAAGGGGTGCCATCAAAAGAAAGGCAGTGATTTTGAAGAAATATTCTCACCGGTGGTGAAGATGACATTCATTAGAGATCGTTCGAGAAGAATGTTGTGGATGTCACAAGAAGATTATCTCAAGAAAGTTCTTGAAAGGTTCAACATGCATAATGCAAAACCTGTGCATGTTCCGTTACCTGGTCATATGAAACTTAGTAAGACACAATGTCCTGAGAATGACCAAGAGAAAGAAGAGATGAGCAAGGTGCCCTATTCTTCAGCTGTGGGTAGCCTTATGTACGCCATGGTCTGCACGAGGCCAGACATCGGTTATGCTGTAGGAGTCGTGAGTAGATTCCTATCAAACCCAGTAAAAGAACACTGGAGTGCTGTGAAATGGATACTGAGGTATCTAGGAGGAACTGCCAAAAAGTGCTTATGTTTTGGCAATGAATATCAAATGCTCGCTGGATATGTGGATGACAGAGATCATCAACCATATGTGAGATATCCTAGTTCGAAATTCATCATCATCACTGATAAAGGAGAGTCCTGGTTTGACGAAAGGTTCGACCGGCTCCTTGTGTCGAAAGTCCTCCTGACTAAGGGTGTTCAGGCGGCGGGTTCCGTTGAATGCGACAACGGCGGTACAAGTATAATTGTGTGCGGATGAAAGAGCTTCCGCTTGAGGGATGAGTATGATGAATTGCGAGGATAGTTTTCCTTTGGCTGGAGGGGGAGAATTGTTGGGGTCCCACCATGTGGAGAAAAGTTCCAGCCGAAAGGCAAAAAGGAAAAATACAAATTGCCACTTGCCTTATGAAACTTCACGTGAAGCAGCAAAGGAAGAAAGCAATTTGCCAACTTGCACAACAACAACAACATGACAGCTTCCAAAAAGGAAAAGACAAAATGCAAATTGCATTTGCGGTGCATAGTAAGAAAATTTGTGAACAAACAGTAGCAAATTTTCTTCTATAAATAGAAGCCAGTTTCAGCACAATTGAAACACCACAACACACAGAGATGTGAGACAACAACACAATCCTCACTCTCACGAAGAGAGTTGAGAGAAAGAGATCCTCACACAGAGTTGTGAGAAGAGAGAGAAAAAAATAGAGAGTTCCTCGTAAGAGTGTGAGAGAAAAATATAGTGAGCCAATTTGTGAGAGTTGTATCTACAAATTGAGATACATAGTTATGTCCACCTGATGGTGAAGATTTGTATTCCCATTATTATTATAGTGGAGATTTAAGAGGCCTACGGGTCCCGTGGTTTTTACTTACTCACATTGAGAAGGTTTTCCACGCTAAAAACTTGTTGTGTGTCATTTTACGTTTCCGCTAAAGTGCCTATTTTGAGTTCACGGAGGAGGGAAATTATGCAGGCATTTCCCAACATTCTCTACGCCGGATGACGGAGAAGATTCAATAATCCGACAGGTGGTCGACGATGAAGAAGTGCGGTTGGGAGCAGAGCATCACTTTAACCTATTTAAACATAAGTTTGGGAAGGTTTATTCTTCCAAGGATGAACATGATTACAGGTTTAAAATCTTCAAGTCTAATCTGAACCGTGCTAAGAAGCATCAATTGATGGATCCTTCGGCGGTTCACGGTGTTACGAAATTCTCCGATCTTACTCCGAGGGAGTTTCGTCAGAGCGTTTTGGGATTGAGAGGGGTGGGGTTGCCGAAAGATGCAAATGCAGCCCCGATTCTTCCGACAGATAATCTTCCGGAGGATTTTGATTGGAGAGAGAAAGGAGCAGTTACTGCTGTTAAAAATCAGGTATTATTTCTAATTGTGATTATGATTATTATTTGTTTATTATTGTTGTTTCGTTTAAAAAAAACAAATTTTGATTGTGTTGGGTTTGTGATCATGAATTAGGGCTCGTGTGGATCATGTTGGAGTTTCAGTACAACCGGTGCACTTGAAGGTGCTCACTTTCTCTCGACCGGAAAACTTGTCAGTCTTAGCGAGCAACAACTTGTCGATTGTGATCATGAGGTTTTTTCTTCTTTTCCTATTTTCCCTTTGTAATTAATAGTATATCTGCTAAGGAATTATGATTATGATTATAATTGTGATTGTCATACTGCATTTTGTAAAGAACTTTATCTGATATTAATCTTTCTAGGAAAACAAGTAACAAAAATAACATTTAGTAACCAAACTCTATTAGATATTGTATGAGGTTCGACTTAAGGAATCTCTGCAGTTGCACATCGATAGTGACTTGTAGGAAAGAAGACAAAAACACGTACAATAAGCAAATTTGAAACATGATTAGGTCATATGAATTTGTTTTAGTAATTGAACATATTTCAAATTAATTAATCTTTACAGAATTATTTATTGGGAAATGCTAACTTGTAATATTTGGGGCTTTATAGCAAGTTATATTATTATATGTTATCTAAATTGGTTTGGATTGGATTTACTTTTATGTATAGTGTGATCCAGAGCAACCGGGTTCTTGTGATGCCGGGTGCAATGGTGGTCTGATGAATAGTGCTTTTGAGTACATTTTAAAATCCGGTGGGGTGATGCGCGAAGAAGACTATCCTTATTCTGGTACTGATCGTGGAGCATGCAAGTTTGACAAGAAAAAAATTGCAGCATCAGTTGCAAATTTCAGCGTTGTTTCACTTGATGAAGACCAGATTGCCGCTAATCTTGTCAAGAACGGTCCTCTTGCTAGTAAGTGCTAAAGTCTTAACAATTTGTGTTTTTTTGTTCAATTATAATTATCGAATTCAAATGCAGATATTGATTCTTGTAAATTTCAATGTGCACAAACGCAGTTGCCTTAAATGCAGTATATATGCAAACATACATTGGAGGAGTATCATGCCCATACATATGCTCAAAGAGACTAGATCATGGAGTGTTACTGGTGGGATATGGATCTGGAGCCTATTCGCCTATTAGGATGAAGGAGAAGCCATATTGGATAATTAAAAACTCATGGGGTGAGACTTGGGGAGAAAATGGATACTACAAAATATGCAGGGGACGAAATATATGCGGAGTGGACTCCATGGTTTCTACTGTGGCTGCCGTTCATACTACTACTCAGTAG

Protein sequence

>MS.gene50738.t1

MIIARGKNEGSLYVMQGKINKGDINVSQDMSKELWHKRLGHMSEKSLEILAKNHLPNIKEPQLRRSTRSRQPSSRYFSGEYVNFTEKGEPQSFMEAIETTNKEKWLRSMEEEMQSLKENQTYDLVKLPDGKRALKNKDRSRRMLWMSQEDYLKKVLERFNMHNAKPVHVPLPGHMKLSKTQCPENDQEKEEMSKVPYSSAVGSLMYAMVCTRPDIGYAVGVVVDDEEVRLGAEHHFNLFKHKFGKVYSSKDEHDYRFKIFKSNLNRAKKHQLMDPSAVHGVTKFSDLTPREFRQSVLGLRGDFDWREKGAVTAVKNQGSCGSCWSFSTTGALEGAHFLSTGKLVSLSEQQLVDCDHECDPEQPGSCDAGCNGGLMNSAFEYILKSGGVMREEDYPYSGTDRGACKFDKKKIAASVANFSVVSLDEDQIAANLVKNGPLAIALNAVYMQTYIGGVSCPYICSKRLDHGVLLVGYGSGAYSPIRMKEKPYWIIKNSWGETWGENGYYKICRGRNICGVDSMVSTVAAVHTTTQ