AlfalfaGEDB | MS.gene069978

M. sativa cultivar XinJiangDaYe / MS.gene069978

KEGG: K16290
GO: GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00112.26 PF08246.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069978.t1	XP_003603871.1	99.1	350	3	0	1	350	1	350	2.00E-201	711.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069978.t1	Q9LM66	73.6	356	88	3	1	350	1	356	2.3e-150	533.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069978.t1	G7J6G3	99.1	350	3	0	1	350	1	350	1.4e-201	711.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene069978	MS.gene055760	PPI
MS.gene069978	MS.gene052630	PPI
MS.gene028850	MS.gene069978	PPI
MS.gene069978	MS.gene045525	PPI
MS.gene069978	MS.gene50738	PPI
MS.gene069978	MS.gene006665	PPI
MS.gene031580	MS.gene069978	PPI
MS.gene069978	MS.gene003680	PPI
MS.gene51064	MS.gene069978	PPI
MS.gene069978	MS.gene003677	PPI
MS.gene069978	MS.gene26670	PPI
MS.gene069978	MS.gene63719	PPI
MS.gene069978	MS.gene005934	PPI
MS.gene026876	MS.gene069978	PPI
MS.gene069978	MS.gene26669	PPI
MS.gene23321	MS.gene069978	PPI
MS.gene069978	MS.gene065787	PPI
MS.gene069978	MS.gene78715	PPI
MS.gene69195	MS.gene069978	PPI
MS.gene069978	MS.gene059966	PPI
MS.gene069978	MS.gene59493	PPI
MS.gene069978	MS.gene73817	PPI
MS.gene069978	MS.gene011363	PPI
MS.gene069978	MS.gene63396	PPI
MS.gene069978	MS.gene052628	PPI
MS.gene069978	MS.gene34090	PPI
MS.gene069978	MS.gene64795	PPI
MS.gene069978	MS.gene73835	PPI
MS.gene069978	MS.gene005933	PPI
MS.gene069978	MS.gene48296	PPI
MS.gene069978	MS.gene73840	PPI
MS.gene069978	MS.gene045461	PPI
MS.gene069978	MS.gene026873	PPI
MS.gene069978	MS.gene033324	PPI
MS.gene069978	MS.gene09529	PPI
MS.gene027420	MS.gene069978	PPI
MS.gene069978	MS.gene033151	PPI
MS.gene73834	MS.gene069978	PPI
MS.gene069978	MS.gene045455	PPI
MS.gene069978	MS.gene24818	PPI
MS.gene069978	MS.gene87639	PPI
MS.gene006682	MS.gene069978	PPI
MS.gene29082	MS.gene069978	PPI
MS.gene069978	MS.gene031638	PPI
MS.gene026874	MS.gene069978	PPI
MS.gene069978	MS.gene26853	PPI
MS.gene069978	MS.gene031619	PPI
MS.gene069978	MS.gene045526	PPI
MS.gene069978	MS.gene031621	PPI
MS.gene069978	MS.gene045520	PPI
MS.gene069978	MS.gene67468	PPI
MS.gene069978	MS.gene73838	PPI
MS.gene069978	MS.gene045503	PPI
MS.gene069978	MS.gene043473	PPI
MS.gene069978	MS.gene031581	PPI
MS.gene069978	MS.gene045522	PPI
MS.gene069978	MS.gene25255	PPI
MS.gene065785	MS.gene069978	PPI
MS.gene069978	MS.gene031635	PPI
MS.gene069978	MS.gene022410	PPI
MS.gene069978	MS.gene006680	PPI
MS.gene060218	MS.gene069978	PPI
MS.gene069978	MS.gene045459	PPI
MS.gene069978	MS.gene058156	PPI
MS.gene069978	MS.gene31410	PPI
MS.gene069978	MS.gene045458	PPI
MS.gene006646	MS.gene069978	PPI
MS.gene069978	MS.gene73815	PPI
MS.gene069978	MS.gene95543	PPI
MS.gene069978	MS.gene006720	PPI
MS.gene069978	MS.gene045501	PPI
MS.gene069978	MS.gene012206	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069978.t1	MTR_3g116080	99.143	350	3	0	1	350	1	350	0.0	721
MS.gene069978.t1	MTR_1g107550	57.100	331	135	5	26	349	27	357	2.25e-133	389
MS.gene069978.t1	MTR_1g018840	50.700	357	164	5	4	348	6	362	2.94e-126	370
MS.gene069978.t1	MTR_1g018840	50.700	357	164	5	4	348	6	362	3.84e-126	370
MS.gene069978.t1	MTR_3g102220	52.260	354	155	7	9	349	11	363	4.13e-123	363
MS.gene069978.t1	MTR_4g104370	53.161	348	151	7	7	349	5	345	1.54e-122	357
MS.gene069978.t1	MTR_3g075390	51.862	349	157	5	5	348	3	345	1.82e-122	357
MS.gene069978.t1	MTR_4g094918	49.128	344	164	4	11	348	4	342	4.09e-120	351
MS.gene069978.t1	MTR_4g079770	51.156	346	154	7	9	347	7	344	1.95e-119	348
MS.gene069978.t1	MTR_4g079800	53.526	312	134	5	43	347	37	344	2.82e-119	348
MS.gene069978.t1	MTR_4g047610	54.921	315	130	6	41	347	32	342	7.19e-119	347
MS.gene069978.t1	MTR_4g079470	53.145	318	138	5	37	347	31	344	1.16e-118	347
MS.gene069978.t1	MTR_4g080700	55.663	309	128	6	43	347	36	339	9.07e-117	342
MS.gene069978.t1	MTR_5g022560	54.984	311	128	6	45	347	37	343	1.06e-116	342
MS.gene069978.t1	MTR_4g107930	53.481	316	135	6	40	347	33	344	7.22e-116	340
MS.gene069978.t1	MTR_4g080730	55.016	309	130	6	43	347	36	339	2.96e-114	335
MS.gene069978.t1	MTR_3g112430	53.074	309	140	3	45	348	34	342	3.63e-112	333
MS.gene069978.t1	MTR_2g075830	53.822	314	132	6	41	347	33	340	8.00e-110	324
MS.gene069978.t1	MTR_4g079440	51.438	313	143	4	36	346	38	343	1.79e-109	323
MS.gene069978.t1	MTR_2g016180	48.837	344	166	6	13	349	17	357	9.93e-109	327
MS.gene069978.t1	MTR_2g016270	48.397	343	154	6	13	349	17	342	5.44e-107	322
MS.gene069978.t1	MTR_2g083930	49.521	313	150	5	42	346	30	342	2.06e-105	313
MS.gene069978.t1	MTR_4g080360	51.133	309	135	6	48	346	38	340	2.47e-103	308
MS.gene069978.t1	MTR_2g090890	49.175	303	149	3	47	348	39	337	1.05e-100	301
MS.gene069978.t1	MTR_7g078600	47.249	309	148	8	43	346	38	336	8.88e-94	283
MS.gene069978.t1	MTR_7g079230	46.753	308	152	7	43	346	35	334	2.44e-89	272
MS.gene069978.t1	MTR_2g015920	40.057	352	194	11	9	348	13	359	1.22e-83	258
MS.gene069978.t1	MTR_4g065077	40.833	360	193	12	1	348	6	357	9.52e-79	245
MS.gene069978.t1	MTR_2g015850	46.758	293	147	7	59	346	74	362	9.90e-78	243
MS.gene069978.t1	MTR_2g015930	40.000	355	191	13	9	348	12	359	2.05e-76	239
MS.gene069978.t1	MTR_8g086470	38.764	356	190	11	1	348	22	357	4.15e-72	228
MS.gene069978.t1	MTR_4g125300	41.042	307	166	9	47	347	51	348	1.57e-68	219
MS.gene069978.t1	MTR_1g023210	39.365	315	166	8	47	344	51	357	1.65e-64	209
MS.gene069978.t1	MTR_4g071060	38.413	315	168	9	47	344	48	353	4.48e-63	205
MS.gene069978.t1	MTR_2g067900	41.667	312	163	10	48	345	17	323	5.03e-62	201
MS.gene069978.t1	MTR_1g094135	36.571	350	192	8	17	344	22	363	1.30e-60	199
MS.gene069978.t1	MTR_7g078630	43.636	275	112	8	41	302	700	944	1.74e-58	203
MS.gene069978.t1	MTR_2g064475	37.209	344	163	13	48	347	17	351	8.79e-58	191
MS.gene069978.t1	MTR_2g067560	41.139	316	153	13	47	339	16	321	8.64e-56	196
MS.gene069978.t1	MTR_1g023810	41.494	241	131	8	110	345	26	261	3.52e-54	179
MS.gene069978.t1	MTR_4g072790	37.183	355	163	13	7	344	6	317	6.70e-54	180
MS.gene069978.t1	MTR_2g066980	36.137	321	147	12	48	347	35	318	5.60e-53	177
MS.gene069978.t1	MTR_4g073660	35.655	359	167	13	7	344	5	320	4.75e-51	173
MS.gene069978.t1	MTR_4g072340	35.294	357	171	13	7	344	6	321	5.98e-50	170
MS.gene069978.t1	MTR_2g016380	50.413	121	56	2	232	349	18	137	2.70e-33	124
MS.gene069978.t1	MTR_1g025450	39.394	198	95	8	113	305	35	212	7.06e-33	128
MS.gene069978.t1	MTR_7g078580	56.122	98	41	2	250	346	4	100	3.85e-32	117
MS.gene069978.t1	MTR_2g015840	27.778	306	157	8	43	347	29	271	1.99e-30	117
MS.gene069978.t1	MTR_7g079130	54.444	90	40	1	258	346	7	96	1.63e-27	104
MS.gene069978.t1	MTR_7g078310	42.222	135	68	4	90	221	117	244	9.27e-25	101
MS.gene069978.t1	MTR_2g047960	56.962	79	34	0	141	219	7	85	7.24e-22	89.4
MS.gene069978.t1	MTR_3g029560	30.078	256	144	10	103	346	4	236	3.93e-21	91.3
MS.gene069978.t1	MTR_7g111060	27.839	273	162	12	94	339	66	330	1.88e-19	88.6
MS.gene069978.t1	MTR_7g111050	26.667	330	204	15	39	339	7	327	3.31e-17	82.0
MS.gene069978.t1	MTR_8g087510	34.615	130	79	4	173	300	23	148	3.68e-14	70.1
MS.gene069978.t1	MTR_7g079260	44.444	72	23	2	274	344	7	62	1.56e-11	60.8
MS.gene069978.t1	MTR_7g111050	25.510	294	187	12	43	309	8	296	9.38e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069978.t1	AT1G20850	77.439	328	72	2	25	350	29	356	0.0	530
MS.gene069978.t1	AT4G35350	69.014	355	105	2	1	350	1	355	0.0	529
MS.gene069978.t1	AT4G35350	66.901	284	89	2	1	279	1	284	1.79e-143	407
MS.gene069978.t1	AT5G43060	53.333	360	152	5	1	349	1	355	2.21e-129	378
MS.gene069978.t1	AT1G47128	52.101	357	158	5	1	348	1	353	3.38e-129	378
MS.gene069978.t1	AT5G50260	55.072	345	144	5	9	348	5	343	4.05e-129	374
MS.gene069978.t1	AT3G19390	54.070	344	149	6	8	348	9	346	8.40e-126	369
MS.gene069978.t1	AT3G48340	51.471	340	158	3	15	348	6	344	1.24e-118	347
MS.gene069978.t1	AT1G09850	53.074	309	144	1	41	348	26	334	6.31e-115	340
MS.gene069978.t1	AT4G36880	50.155	323	149	4	38	348	40	362	4.62e-112	331
MS.gene069978.t1	AT5G45890	48.974	341	159	7	17	347	10	345	2.39e-111	328
MS.gene069978.t1	AT3G19400	51.948	308	142	6	46	348	43	349	1.39e-108	322
MS.gene069978.t1	AT4G11310	47.175	354	174	6	5	348	3	353	6.88e-108	320
MS.gene069978.t1	AT4G11320	46.348	356	180	6	1	348	8	360	6.81e-107	318
MS.gene069978.t1	AT4G11320	46.348	356	180	6	1	348	8	360	6.81e-107	318
MS.gene069978.t1	AT1G06260	48.538	342	166	5	8	348	11	343	1.48e-106	316
MS.gene069978.t1	AT4G23520	44.729	351	190	4	1	348	1	350	6.72e-106	315
MS.gene069978.t1	AT3G48350	47.688	346	169	6	9	348	5	344	7.72e-106	315
MS.gene069978.t1	AT3G49340	48.232	311	150	6	44	346	32	339	9.60e-100	298
MS.gene069978.t1	AT2G27420	47.634	317	150	6	44	346	32	346	6.34e-98	294
MS.gene069978.t1	AT1G29090	47.771	314	153	6	43	347	43	354	5.24e-96	289
MS.gene069978.t1	AT2G34080	46.474	312	158	5	43	347	35	344	5.06e-95	286
MS.gene069978.t1	AT1G29080	42.566	343	183	9	15	347	7	345	6.04e-92	279
MS.gene069978.t1	AT3G43960	45.370	324	166	9	33	348	27	347	4.55e-86	265
MS.gene069978.t1	AT1G29110	37.647	340	190	6	17	347	7	333	1.20e-80	249
MS.gene069978.t1	AT3G19400	51.899	237	108	6	46	277	43	278	5.94e-77	238
MS.gene069978.t1	AT3G48350	45.161	279	142	5	9	282	5	277	7.86e-75	233
MS.gene069978.t1	AT5G60360	42.671	307	161	9	47	347	59	356	1.59e-74	234
MS.gene069978.t1	AT5G60360	42.345	307	161	10	47	347	59	355	6.43e-72	228
MS.gene069978.t1	AT5G60360	42.667	300	157	9	47	340	59	349	9.03e-72	228
MS.gene069978.t1	AT4G39090	39.940	333	171	9	29	344	37	357	2.75e-70	224
MS.gene069978.t1	AT2G21430	39.634	328	169	9	34	344	39	354	2.82e-69	221
MS.gene069978.t1	AT3G45310	40.717	307	167	9	47	347	59	356	2.81e-67	216
MS.gene069978.t1	AT3G45310	39.474	304	173	7	47	344	59	357	5.05e-65	210
MS.gene069978.t1	AT4G16190	39.241	316	167	8	47	344	55	363	3.52e-61	201
MS.gene069978.t1	AT3G54940	35.655	359	199	9	10	344	9	359	2.39e-60	198
MS.gene069978.t1	AT3G54940	34.201	269	162	4	10	264	9	276	9.51e-46	157
MS.gene069978.t1	AT4G01610	26.562	320	193	15	59	349	36	342	1.21e-16	80.5
MS.gene069978.t1	AT4G01610	26.250	320	194	15	59	349	36	342	1.77e-15	77.0

Find 89 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCAGGTTCGAGGTCTTTA+AGG	0.183217	3.2:+91754860	MS.gene069978:CDS
ACTGATGTTGTGACTATTAA+TGG	0.222069	3.2:+91755892	MS.gene069978:CDS
TCAAATTCCCTGTCACAATT+TGG	0.244699	3.2:-91755594	None:intergenic
GCAGGTCAGGACAAGTGTTT+TGG	0.258810	3.2:-91754682	None:intergenic
GGAAGATTATCCTTACATTA+TGG	0.312016	3.2:+91755735	MS.gene069978:CDS
ATCTCTGCCTGAAGCCTCTA+TGG	0.319266	3.2:-91755986	None:intergenic
ACTTGTTCTTGAACTCTTGA+TGG	0.328420	3.2:-91754962	None:intergenic
ATATGGTACATCAAAGGGTT+TGG	0.369364	3.2:+91756176	MS.gene069978:CDS
TTATTTCTATCTTTAGCATT+TGG	0.374681	3.2:+91754714	MS.gene069978:CDS
CATTTGTAGGGTGTGTTTGA+TGG	0.384261	3.2:+91756111	MS.gene069978:intron
AAGCACTTGCGAGATGAAAA+AGG	0.391386	3.2:+91755762	MS.gene069978:CDS
GAGTACTTGCAGGTAGTTGC+TGG	0.397924	3.2:+91755536	MS.gene069978:intron
AACATACTCCACTATAGAAC+TGG	0.404978	3.2:-91756011	None:intergenic
CATTGACCTTGATTCTTGAC+CGG	0.416938	3.2:-91755101	None:intergenic
ACATTATAGTGAAGAATTCA+TGG	0.418831	3.2:+91756202	MS.gene069978:CDS
TTTCTCCAATCAACTGACTT+AGG	0.421540	3.2:-91755062	None:intergenic
TCAACTGTTGCCGCGGTTGA+AGG	0.428053	3.2:+91755565	MS.gene069978:CDS
ATAAGCATAAGGAGCAGGTC+AGG	0.428907	3.2:-91754695	None:intergenic
GGAGCAAAGTGGGGTGAGAA+AGG	0.429527	3.2:+91756225	MS.gene069978:CDS
ATTTGCCTAAGTCAGTTGAT+TGG	0.430861	3.2:+91755057	MS.gene069978:CDS
CTTACAACAATGGTTGCAAT+GGG	0.434061	3.2:+91755656	MS.gene069978:CDS
GGGGTGAGAAAGGGTTTATA+AGG	0.436382	3.2:+91756235	MS.gene069978:CDS
GGTGTGTCAGCCGTTGGATA+TGG	0.454497	3.2:+91756159	MS.gene069978:CDS
AGTTCAAGAACAAGTATCTT+GGG	0.458742	3.2:+91754970	MS.gene069978:CDS
GAACAAGTATCTTGGGCTAA+AGG	0.462245	3.2:+91754977	MS.gene069978:CDS
GAGTTCAAGAACAAGTATCT+TGG	0.466462	3.2:+91754969	MS.gene069978:CDS
AGAGATTTCCAGTTCTATAG+TGG	0.466790	3.2:+91756003	MS.gene069978:CDS
GGAATAAACCAAATTGTGAC+AGG	0.467218	3.2:+91755586	MS.gene069978:CDS
GGAAAGTCTGAAGGGATTTG+TGG	0.476760	3.2:+91756273	MS.gene069978:CDS
TCATTGAACTCTTCGAATCA+TGG	0.479297	3.2:+91754793	MS.gene069978:CDS
GAATTCATGGGGAGCAAAGT+GGG	0.481502	3.2:+91756215	MS.gene069978:CDS
TCCATGGACTTCAAGTCCTC+TGG	0.486674	3.2:-91754765	None:intergenic
TAATGAACAAAGCTTATTGA+AGG	0.491336	3.2:+91755936	MS.gene069978:CDS
GAGCAAAGTGGGGTGAGAAA+GGG	0.494121	3.2:+91756226	MS.gene069978:CDS
ATCTAAAGCACATTGATGAT+AGG	0.494574	3.2:+91754886	MS.gene069978:CDS
GTGCTTTCCTCCATAATGTA+AGG	0.494914	3.2:-91755745	None:intergenic
TAGAAATAAGCATAAGGAGC+AGG	0.496949	3.2:-91754700	None:intergenic
ACAAGGTTGTCAGCAACTAC+TGG	0.500291	3.2:+91754910	MS.gene069978:CDS
GTTGTCAGCAACTACTGGCT+TGG	0.503373	3.2:+91754915	MS.gene069978:CDS
ATTGACCTTGATTCTTGACC+GGG	0.509041	3.2:-91755100	None:intergenic
TCAGTTGATTGGAGAAAGAA+AGG	0.509647	3.2:+91755068	MS.gene069978:CDS
TAAAGATAGAAATAAGCATA+AGG	0.512917	3.2:-91754706	None:intergenic
GTTGGATATGGTACATCAAA+GGG	0.516544	3.2:+91756171	MS.gene069978:CDS
CTATGGCCACACTGAGTGGT+TGG	0.516563	3.2:-91755969	None:intergenic
CGTTGGATATGGTACATCAA+AGG	0.524173	3.2:+91756170	MS.gene069978:CDS
ATAAGGATGAAGAGAAACAT+TGG	0.526478	3.2:+91756252	MS.gene069978:CDS
AGAAACATTGGAAAGTCTGA+AGG	0.526996	3.2:+91756264	MS.gene069978:CDS
CATTATAGTGAAGAATTCAT+GGG	0.532840	3.2:+91756203	MS.gene069978:CDS
ACTTACAACAATGGTTGCAA+TGG	0.533682	3.2:+91755655	MS.gene069978:CDS
GGACGTGATTTCTCCATCGT+GGG	0.535021	3.2:+91754735	MS.gene069978:CDS
TACAACAATGGTTGCAATGG+GGG	0.543003	3.2:+91755658	MS.gene069978:CDS
ACCACTCAGTGTGGCCATAG+AGG	0.545434	3.2:+91755972	MS.gene069978:CDS
AAAGAAAGGTGCTGTAACCC+CGG	0.549002	3.2:+91755082	MS.gene069978:CDS
TAAAGGTTGACTTGTCTCAA+AGG	0.550091	3.2:+91754994	MS.gene069978:CDS
GATCATGGTGTGTCAGCCGT+TGG	0.556769	3.2:+91756153	MS.gene069978:CDS
GTAACCCCGGTCAAGAATCA+AGG	0.557737	3.2:+91755095	MS.gene069978:CDS
AAACGGTGGACTACATAAAG+AGG	0.559559	3.2:+91755714	MS.gene069978:CDS
AGTGTGGCCATAGAGGCTTC+AGG	0.567247	3.2:+91755979	MS.gene069978:CDS
GAGTTCAATGAGCTTATCCA+TGG	0.571450	3.2:-91754781	None:intergenic
ACCAGAGGACTTGAAGTCCA+TGG	0.576800	3.2:+91754764	MS.gene069978:CDS
TGGACGTGATTTCTCCATCG+TGG	0.576917	3.2:+91754734	MS.gene069978:CDS
TGCGGAAGTGAACTAGATCA+TGG	0.579661	3.2:+91756138	MS.gene069978:CDS
CCATTGAAGAAAAGCTACTC+AGG	0.581738	3.2:+91754844	MS.gene069978:CDS
AGAAAAGCTACTCAGGTTCG+AGG	0.582875	3.2:+91754851	MS.gene069978:CDS
AAGGAGAGAGTCCTCAGAAG+AGG	0.591057	3.2:+91755013	MS.gene069978:CDS
AGAATTCATGGGGAGCAAAG+TGG	0.591803	3.2:+91756214	MS.gene069978:CDS
GGCCACACTGAGTGGTTGGT+TGG	0.597846	3.2:-91755965	None:intergenic
ATAAGTGAATTCCTCTTCTG+AGG	0.598495	3.2:-91755024	None:intergenic
GCAGTCGATTAATTCTTGCT+CGG	0.604512	3.2:-91755626	None:intergenic
AGTACTTGCAGGTAGTTGCT+GGG	0.604719	3.2:+91755537	MS.gene069978:intron
GCCTCTATGGCCACACTGAG+TGG	0.610194	3.2:-91755973	None:intergenic
TTGGTTTATTCCTTCAACCG+CGG	0.610274	3.2:-91755575	None:intergenic
AGATTATCCTTACATTATGG+AGG	0.615877	3.2:+91755738	MS.gene069978:CDS
GAAACATTGGAAAGTCTGAA+GGG	0.619388	3.2:+91756265	MS.gene069978:CDS
GATTTGTGGACTCTACAAGA+TGG	0.625513	3.2:+91756287	MS.gene069978:CDS
CTTTGATGTACCATATCCAA+CGG	0.626421	3.2:-91756169	None:intergenic
TGCGACACAACTTACAACAA+TGG	0.635197	3.2:+91755646	MS.gene069978:CDS
GAATCATGGATGTCGAGACA+CGG	0.654814	3.2:+91754807	MS.gene069978:CDS
CTCTGGTGAATAGCCCACGA+TGG	0.663678	3.2:-91754748	None:intergenic
ATTATAGTGAAGAATTCATG+GGG	0.665269	3.2:+91756204	MS.gene069978:CDS
GCACATTGATGATAGGAACA+AGG	0.668624	3.2:+91754893	MS.gene069978:CDS
AATTCATGGGGAGCAAAGTG+GGG	0.670875	3.2:+91756216	MS.gene069978:CDS
TGCCAACCAACCACTCAGTG+TGG	0.678227	3.2:+91755963	MS.gene069978:CDS
CATCGTGGGCTATTCACCAG+AGG	0.679810	3.2:+91754749	MS.gene069978:CDS
GTCAAGAATCAAGGTCAATG+TGG	0.700857	3.2:+91755104	MS.gene069978:CDS
GAATAAACCAAATTGTGACA+GGG	0.702620	3.2:+91755587	MS.gene069978:CDS
TTACAACAATGGTTGCAATG+GGG	0.703567	3.2:+91755657	MS.gene069978:CDS
TTGACCTTGATTCTTGACCG+GGG	0.717921	3.2:-91755099	None:intergenic
GGTGTGTTTGATGGACATTG+CGG	0.757484	3.2:+91756120	MS.gene069978:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATATATACTATTGGATAA+CGG	+	chr3.2:91755444-91755463	MS.gene069978:intron	15.0%
!!	ATAAGTAACATAACTTTCAT+TGG	+	chr3.2:91755850-91755869	MS.gene069978:intron	20.0%
!!	ATTATTTAACTACCATTTGT+AGG	+	chr3.2:91756098-91756117	MS.gene069978:intron	20.0%
!!	GTTCAGTTTATATATACTAT+TGG	+	chr3.2:91755437-91755456	MS.gene069978:intron	20.0%
!!	TAAAGATAGAAATAAGCATA+AGG	-	chr3.2:91754709-91754728	None:intergenic	20.0%
!!	TTATTTAACTACCATTTGTA+GGG	+	chr3.2:91756099-91756118	MS.gene069978:intron	20.0%
!!!	TTATTTCTATCTTTAGCATT+TGG	+	chr3.2:91754714-91754733	MS.gene069978:CDS	20.0%
!	ACATTATAGTGAAGAATTCA+TGG	+	chr3.2:91756202-91756221	MS.gene069978:CDS	25.0%
!	ATCATTGAATTGATATCAGA+CGG	-	chr3.2:91755301-91755320	None:intergenic	25.0%
!	ATTATAGTGAAGAATTCATG+GGG	+	chr3.2:91756204-91756223	MS.gene069978:CDS	25.0%
!	CATTATAGTGAAGAATTCAT+GGG	+	chr3.2:91756203-91756222	MS.gene069978:CDS	25.0%
!	TAATGAACAAAGCTTATTGA+AGG	+	chr3.2:91755936-91755955	MS.gene069978:CDS	25.0%
!	TTCTCCTTCATTGTAAAAAA+CGG	+	chr3.2:91755697-91755716	MS.gene069978:CDS	25.0%
!!!	AAGCTTTGTTCATTATTTTG+TGG	-	chr3.2:91755931-91755950	None:intergenic	25.0%
	ACTGATGTTGTGACTATTAA+TGG	+	chr3.2:91755892-91755911	MS.gene069978:CDS	30.0%
	AGATTATCCTTACATTATGG+AGG	+	chr3.2:91755738-91755757	MS.gene069978:CDS	30.0%
	AGCGAAAAATTTGCATGATT+AGG	-	chr3.2:91755381-91755400	None:intergenic	30.0%
	AGTTCAAGAACAAGTATCTT+GGG	+	chr3.2:91754970-91754989	MS.gene069978:CDS	30.0%
	ATAAGGATGAAGAGAAACAT+TGG	+	chr3.2:91756252-91756271	MS.gene069978:CDS	30.0%
	GAATAAACCAAATTGTGACA+GGG	+	chr3.2:91755587-91755606	MS.gene069978:CDS	30.0%
	GGAAGATTATCCTTACATTA+TGG	+	chr3.2:91755735-91755754	MS.gene069978:CDS	30.0%
	TAGGCATGTTTACATATAGT+AGG	-	chr3.2:91755362-91755381	None:intergenic	30.0%
	TGGGTGAATCTAATCTTAAA+TGG	+	chr3.2:91756061-91756080	MS.gene069978:intron	30.0%
!	ATCTAAAGCACATTGATGAT+AGG	+	chr3.2:91754886-91754905	MS.gene069978:CDS	30.0%
	AACATACTCCACTATAGAAC+TGG	-	chr3.2:91756014-91756033	None:intergenic	35.0%
	ACTTACAACAATGGTTGCAA+TGG	+	chr3.2:91755655-91755674	MS.gene069978:CDS	35.0%
	ACTTGTTCTTGAACTCTTGA+TGG	-	chr3.2:91754965-91754984	None:intergenic	35.0%
	AGAAACATTGGAAAGTCTGA+AGG	+	chr3.2:91756264-91756283	MS.gene069978:CDS	35.0%
	AGAGATTTCCAGTTCTATAG+TGG	+	chr3.2:91756003-91756022	MS.gene069978:CDS	35.0%
	ATAAGTGAATTCCTCTTCTG+AGG	-	chr3.2:91755027-91755046	None:intergenic	35.0%
	ATATGGTACATCAAAGGGTT+TGG	+	chr3.2:91756176-91756195	MS.gene069978:CDS	35.0%
	ATTTGCCTAAGTCAGTTGAT+TGG	+	chr3.2:91755057-91755076	MS.gene069978:CDS	35.0%
	CTTACAACAATGGTTGCAAT+GGG	+	chr3.2:91755656-91755675	MS.gene069978:CDS	35.0%
	CTTTGATGTACCATATCCAA+CGG	-	chr3.2:91756172-91756191	None:intergenic	35.0%
	GAAACATTGGAAAGTCTGAA+GGG	+	chr3.2:91756265-91756284	MS.gene069978:CDS	35.0%
	GAGTTCAAGAACAAGTATCT+TGG	+	chr3.2:91754969-91754988	MS.gene069978:CDS	35.0%
	GGAATAAACCAAATTGTGAC+AGG	+	chr3.2:91755586-91755605	MS.gene069978:CDS	35.0%
	GTTGGATATGGTACATCAAA+GGG	+	chr3.2:91756171-91756190	MS.gene069978:CDS	35.0%
	TAGAAATAAGCATAAGGAGC+AGG	-	chr3.2:91754703-91754722	None:intergenic	35.0%
	TCAAATTCCCTGTCACAATT+TGG	-	chr3.2:91755597-91755616	None:intergenic	35.0%
	TCAGTTGATTGGAGAAAGAA+AGG	+	chr3.2:91755068-91755087	MS.gene069978:CDS	35.0%
	TCATTGAACTCTTCGAATCA+TGG	+	chr3.2:91754793-91754812	MS.gene069978:CDS	35.0%
	TCCTTCATTGTAAAAAACGG+TGG	+	chr3.2:91755700-91755719	MS.gene069978:CDS	35.0%
	TTACAACAATGGTTGCAATG+GGG	+	chr3.2:91755657-91755676	MS.gene069978:CDS	35.0%
!	TTTCTCCAATCAACTGACTT+AGG	-	chr3.2:91755065-91755084	None:intergenic	35.0%
!!	TAAAGGTTGACTTGTCTCAA+AGG	+	chr3.2:91754994-91755013	MS.gene069978:CDS	35.0%
!!	TTTCATTGGTTTGTTTCACC+AGG	+	chr3.2:91755864-91755883	MS.gene069978:intron	35.0%
!!!	TCCACCGTTTTTTACAATGA+AGG	-	chr3.2:91755704-91755723	None:intergenic	35.0%
	AAGCACTTGCGAGATGAAAA+AGG	+	chr3.2:91755762-91755781	MS.gene069978:CDS	40.0%
	AGCTTGCAAGCATATTGACA+TGG	+	chr3.2:91755501-91755520	MS.gene069978:intron	40.0%
	ATTGACCTTGATTCTTGACC+GGG	-	chr3.2:91755103-91755122	None:intergenic	40.0%
	CATCAAACACACCCTACAAA+TGG	-	chr3.2:91756113-91756132	None:intergenic	40.0%
	CATTGACCTTGATTCTTGAC+CGG	-	chr3.2:91755104-91755123	None:intergenic	40.0%
	CATTTGTAGGGTGTGTTTGA+TGG	+	chr3.2:91756111-91756130	MS.gene069978:intron	40.0%
	CCATTGAAGAAAAGCTACTC+AGG	+	chr3.2:91754844-91754863	MS.gene069978:CDS	40.0%
	CGTTGGATATGGTACATCAA+AGG	+	chr3.2:91756170-91756189	MS.gene069978:CDS	40.0%
	GAACAAGTATCTTGGGCTAA+AGG	+	chr3.2:91754977-91754996	MS.gene069978:CDS	40.0%
	GAGTTCAATGAGCTTATCCA+TGG	-	chr3.2:91754784-91754803	None:intergenic	40.0%
	GATTTGTGGACTCTACAAGA+TGG	+	chr3.2:91756287-91756306	MS.gene069978:CDS	40.0%
	GCAGTCGATTAATTCTTGCT+CGG	-	chr3.2:91755629-91755648	None:intergenic	40.0%
	GTCAAGAATCAAGGTCAATG+TGG	+	chr3.2:91755104-91755123	MS.gene069978:CDS	40.0%
	GTGCTTTCCTCCATAATGTA+AGG	-	chr3.2:91755748-91755767	None:intergenic	40.0%
	TACAACAATGGTTGCAATGG+GGG	+	chr3.2:91755658-91755677	MS.gene069978:CDS	40.0%
	TCACAACATCAGTTACTTCC+TGG	-	chr3.2:91755885-91755904	None:intergenic	40.0%
	TGCGACACAACTTACAACAA+TGG	+	chr3.2:91755646-91755665	MS.gene069978:CDS	40.0%
	TTGCAAGCATATTGACATGG+TGG	+	chr3.2:91755504-91755523	MS.gene069978:intron	40.0%
	TTGGTTTATTCCTTCAACCG+CGG	-	chr3.2:91755578-91755597	None:intergenic	40.0%
!	CCTGAGTAGCTTTTCTTCAA+TGG	-	chr3.2:91754847-91754866	None:intergenic	40.0%
!	GCACATTGATGATAGGAACA+AGG	+	chr3.2:91754893-91754912	MS.gene069978:CDS	40.0%
!!	AAACGGTGGACTACATAAAG+AGG	+	chr3.2:91755714-91755733	MS.gene069978:CDS	40.0%
	AATTCATGGGGAGCAAAGTG+GGG	+	chr3.2:91756216-91756235	MS.gene069978:CDS	45.0%
	ACAAGGTTGTCAGCAACTAC+TGG	+	chr3.2:91754910-91754929	MS.gene069978:CDS	45.0%
	ACTCAGGTTCGAGGTCTTTA+AGG	+	chr3.2:91754860-91754879	MS.gene069978:CDS	45.0%
	AGAAAAGCTACTCAGGTTCG+AGG	+	chr3.2:91754851-91754870	MS.gene069978:CDS	45.0%
	AGAATTCATGGGGAGCAAAG+TGG	+	chr3.2:91756214-91756233	MS.gene069978:CDS	45.0%
	AGTACTTGCAGGTAGTTGCT+GGG	+	chr3.2:91755537-91755556	MS.gene069978:intron	45.0%
	ATAAGCATAAGGAGCAGGTC+AGG	-	chr3.2:91754698-91754717	None:intergenic	45.0%
	GAATCATGGATGTCGAGACA+CGG	+	chr3.2:91754807-91754826	MS.gene069978:CDS	45.0%
	GAATTCATGGGGAGCAAAGT+GGG	+	chr3.2:91756215-91756234	MS.gene069978:CDS	45.0%
	GGAAAGTCTGAAGGGATTTG+TGG	+	chr3.2:91756273-91756292	MS.gene069978:CDS	45.0%
	GGGGTGAGAAAGGGTTTATA+AGG	+	chr3.2:91756235-91756254	MS.gene069978:CDS	45.0%
	GTATGAACACGAGTACTTGC+AGG	+	chr3.2:91755526-91755545	MS.gene069978:intron	45.0%
	TGCGGAAGTGAACTAGATCA+TGG	+	chr3.2:91756138-91756157	MS.gene069978:CDS	45.0%
	TTGACCTTGATTCTTGACCG+GGG	-	chr3.2:91755102-91755121	None:intergenic	45.0%
!	AGGTCAGGACAAGTGTTTTG+GGG	-	chr3.2:91754683-91754702	None:intergenic	45.0%
!	CAGGTCAGGACAAGTGTTTT+GGG	-	chr3.2:91754684-91754703	None:intergenic	45.0%
!	GGTGTGTTTGATGGACATTG+CGG	+	chr3.2:91756120-91756139	MS.gene069978:CDS	45.0%
!!	AAAGAAAGGTGCTGTAACCC+CGG	+	chr3.2:91755082-91755101	MS.gene069978:CDS	45.0%
!!!	TAATTATTGTTTTTTTTAAT+GGG	+	chr3.2:91756042-91756061	MS.gene069978:intron	5.0%
!!!	TTAATTATTGTTTTTTTTAA+TGG	+	chr3.2:91756041-91756060	MS.gene069978:intron	5.0%
	AAGGAGAGAGTCCTCAGAAG+AGG	+	chr3.2:91755013-91755032	MS.gene069978:CDS	50.0%
	ACCAGAGGACTTGAAGTCCA+TGG	+	chr3.2:91754764-91754783	MS.gene069978:CDS	50.0%
	ATCTCTGCCTGAAGCCTCTA+TGG	-	chr3.2:91755989-91756008	None:intergenic	50.0%
	CATCCACATAGTCGATGCAC+TGG	+	chr3.2:91755182-91755201	MS.gene069978:intron	50.0%
	GAGCAAAGTGGGGTGAGAAA+GGG	+	chr3.2:91756226-91756245	MS.gene069978:CDS	50.0%
	GAGTACTTGCAGGTAGTTGC+TGG	+	chr3.2:91755536-91755555	MS.gene069978:intron	50.0%
	GGACGTGATTTCTCCATCGT+GGG	+	chr3.2:91754735-91754754	MS.gene069978:CDS	50.0%
	GTAACCCCGGTCAAGAATCA+AGG	+	chr3.2:91755095-91755114	MS.gene069978:CDS	50.0%
	TCCATGGACTTCAAGTCCTC+TGG	-	chr3.2:91754768-91754787	None:intergenic	50.0%
	TGGACGTGATTTCTCCATCG+TGG	+	chr3.2:91754734-91754753	MS.gene069978:CDS	50.0%
	TGGTTGCAATGGGGGTCTAA+TGG	+	chr3.2:91755666-91755685	MS.gene069978:CDS	50.0%
!	GCAGGTCAGGACAAGTGTTT+TGG	-	chr3.2:91754685-91754704	None:intergenic	50.0%
!	GGCATTTTCAACTGTTGCCG+CGG	+	chr3.2:91755558-91755577	MS.gene069978:CDS	50.0%
!	GTTGTCAGCAACTACTGGCT+TGG	+	chr3.2:91754915-91754934	MS.gene069978:CDS	50.0%
!!	GAACCAGTGCATCGACTATG+TGG	-	chr3.2:91755188-91755207	None:intergenic	50.0%
!!	GGTCAGGACAAGTGTTTTGG+GGG	-	chr3.2:91754682-91754701	None:intergenic	50.0%
!!	GTGCATCGACTATGTGGATG+CGG	-	chr3.2:91755182-91755201	None:intergenic	50.0%
!!	TGCATCGACTATGTGGATGC+GGG	-	chr3.2:91755181-91755200	None:intergenic	50.0%
	ACCACTCAGTGTGGCCATAG+AGG	+	chr3.2:91755972-91755991	MS.gene069978:CDS	55.0%
	AGTGTGGCCATAGAGGCTTC+AGG	+	chr3.2:91755979-91755998	MS.gene069978:CDS	55.0%
	CTATGGCCACACTGAGTGGT+TGG	-	chr3.2:91755972-91755991	None:intergenic	55.0%
	CTCTGGTGAATAGCCCACGA+TGG	-	chr3.2:91754751-91754770	None:intergenic	55.0%
	GATCATGGTGTGTCAGCCGT+TGG	+	chr3.2:91756153-91756172	MS.gene069978:CDS	55.0%
	GGAGCAAAGTGGGGTGAGAA+AGG	+	chr3.2:91756225-91756244	MS.gene069978:CDS	55.0%
	GGTGTGTCAGCCGTTGGATA+TGG	+	chr3.2:91756159-91756178	MS.gene069978:CDS	55.0%
	TCAACTGTTGCCGCGGTTGA+AGG	+	chr3.2:91755565-91755584	MS.gene069978:CDS	55.0%
	TGCCAACCAACCACTCAGTG+TGG	+	chr3.2:91755963-91755982	MS.gene069978:CDS	55.0%
!	CATCGTGGGCTATTCACCAG+AGG	+	chr3.2:91754749-91754768	MS.gene069978:CDS	55.0%
!!	GCATCGACTATGTGGATGCG+GGG	-	chr3.2:91755180-91755199	None:intergenic	55.0%
	GCCTCTATGGCCACACTGAG+TGG	-	chr3.2:91755976-91755995	None:intergenic	60.0%
	GGCCACACTGAGTGGTTGGT+TGG	-	chr3.2:91755968-91755987	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	91754666	91756335	91754666	ID=MS.gene069978
chr3.2	mRNA	91754666	91756335	91754666	ID=MS.gene069978.t1;Parent=MS.gene069978
chr3.2	exon	91754666	91755125	91754666	ID=MS.gene069978.t1.exon1;Parent=MS.gene069978.t1
chr3.2	CDS	91754666	91755125	91754666	ID=cds.MS.gene069978.t1;Parent=MS.gene069978.t1
chr3.2	exon	91755548	91755783	91755548	ID=MS.gene069978.t1.exon2;Parent=MS.gene069978.t1
chr3.2	CDS	91755548	91755783	91755548	ID=cds.MS.gene069978.t1;Parent=MS.gene069978.t1
chr3.2	exon	91755886	91756026	91755886	ID=MS.gene069978.t1.exon3;Parent=MS.gene069978.t1
chr3.2	CDS	91755886	91756026	91755886	ID=cds.MS.gene069978.t1;Parent=MS.gene069978.t1
chr3.2	exon	91756120	91756335	91756120	ID=MS.gene069978.t1.exon4;Parent=MS.gene069978.t1
chr3.2	CDS	91756120	91756335	91756120	ID=cds.MS.gene069978.t1;Parent=MS.gene069978.t1

Gene Sequence

>MS.gene069978

ATGGCTTTTTTCTCCCCCAAAACACTTGTCCTGACCTGCTCCTTATGCTTATTTCTATCTTTAGCATTTGGACGTGATTTCTCCATCGTGGGCTATTCACCAGAGGACTTGAAGTCCATGGATAAGCTCATTGAACTCTTCGAATCATGGATGTCGAGACACGGAAAGATTTATGAGACCATTGAAGAAAAGCTACTCAGGTTCGAGGTCTTTAAGGATAATCTAAAGCACATTGATGATAGGAACAAGGTTGTCAGCAACTACTGGCTTGGTTTGAATGAGTTTGCTGATTTGAGCCATCAAGAGTTCAAGAACAAGTATCTTGGGCTAAAGGTTGACTTGTCTCAAAGGAGAGAGTCCTCAGAAGAGGAATTCACTTATAGAGATGTTGATTTGCCTAAGTCAGTTGATTGGAGAAAGAAAGGTGCTGTAACCCCGGTCAAGAATCAAGGTCAATGTGGTAAGTATACTATTATTGCTATATATGAAAATCTATCTATTCTTGAATATTCCCCGCATCCACATAGTCGATGCACTGGTTCTCTTTAGATTTAGATCGAACAATCAGAAAATAAGTCTATTTTGATTCTTTTTGATAAGTTAAAATTAAAATACTTGATTTAAAATTTGAACCGTCTGATATCAATTCAATGATTATTGATTTGTTCATTGCATGAATTCAGCGTATTTCATCCTACTATATGTAAACATGCCTAATCATGCAAATTTTTCGCTATATGTTAGATTAATTTTTCAGTTAAATTTGAAATAGTTCAGTTTATATATACTATTGGATAACGGCACACAAGAAAATGCTTAAGTTTTGTTTAGAAATAGCTTGCAAGCATATTGACATGGTGGTATGAACACGAGTACTTGCAGGTAGTTGCTGGGCATTTTCAACTGTTGCCGCGGTTGAAGGAATAAACCAAATTGTGACAGGGAATTTGACATCGTTGTCCGAGCAAGAATTAATCGACTGCGACACAACTTACAACAATGGTTGCAATGGGGGTCTAATGGACTATGCATTCTCCTTCATTGTAAAAAACGGTGGACTACATAAAGAGGAAGATTATCCTTACATTATGGAGGAAAGCACTTGCGAGATGAAAAAGGTACATATAGATTAATTAAGCTAACAAATTCAATCTAAAAACATATTGTTTCATACTAAAGTTATTATAAGTAACATAACTTTCATTGGTTTGTTTCACCAGGAAGTAACTGATGTTGTGACTATTAATGGATATCATGACGTGCCACAAAATAATGAACAAAGCTTATTGAAGGCACTTGCCAACCAACCACTCAGTGTGGCCATAGAGGCTTCAGGCAGAGATTTCCAGTTCTATAGTGGAGTATGTTTAATTAATTAATTATTGTTTTTTTTAATGGGTGAATCTAATCTTAAATGGAATTTTTTGAACTGATTATTTAACTACCATTTGTAGGGTGTGTTTGATGGACATTGCGGAAGTGAACTAGATCATGGTGTGTCAGCCGTTGGATATGGTACATCAAAGGGTTTGGATTACATTATAGTGAAGAATTCATGGGGAGCAAAGTGGGGTGAGAAAGGGTTTATAAGGATGAAGAGAAACATTGGAAAGTCTGAAGGGATTTGTGGACTCTACAAGATGGCTTCTTATCCCACTAAAAAGAAATAA

Protein sequence

>MS.gene069978.t1

MAFFSPKTLVLTCSLCLFLSLAFGRDFSIVGYSPEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDDRNKVVSNYWLGLNEFADLSHQEFKNKYLGLKVDLSQRRESSEEEFTYRDVDLPKSVDWRKKGAVTPVKNQGQCGSCWAFSTVAAVEGINQIVTGNLTSLSEQELIDCDTTYNNGCNGGLMDYAFSFIVKNGGLHKEEDYPYIMEESTCEMKKEVTDVVTINGYHDVPQNNEQSLLKALANQPLSVAIEASGRDFQFYSGGVFDGHCGSELDHGVSAVGYGTSKGLDYIIVKNSWGAKWGEKGFIRMKRNIGKSEGICGLYKMASYPTKKK