AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006646

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006646.t1 MTR_4g079440 94.737 304 16 0 1 304 42 345 0.0 606
MS.gene006646.t1 MTR_2g090890 65.017 303 105 1 1 303 35 336 2.58e-149 422
MS.gene006646.t1 MTR_4g107930 57.468 308 125 3 1 302 36 343 4.92e-126 363
MS.gene006646.t1 MTR_5g022560 56.494 308 128 3 1 302 35 342 2.45e-123 357
MS.gene006646.t1 MTR_4g079470 56.209 306 127 4 3 302 39 343 2.60e-122 354
MS.gene006646.t1 MTR_4g079770 56.209 306 127 4 3 302 39 343 3.09e-122 354
MS.gene006646.t1 MTR_4g047610 55.519 308 131 3 1 302 34 341 1.00e-121 352
MS.gene006646.t1 MTR_4g079800 55.556 306 129 4 3 302 39 343 6.94e-120 348
MS.gene006646.t1 MTR_4g080730 53.947 304 137 3 1 302 36 338 2.35e-118 344
MS.gene006646.t1 MTR_4g080700 52.961 304 140 3 1 302 36 338 1.39e-116 339
MS.gene006646.t1 MTR_3g075390 53.398 309 130 5 5 303 40 344 2.75e-114 334
MS.gene006646.t1 MTR_1g107550 54.313 313 130 5 1 303 46 355 7.77e-114 337
MS.gene006646.t1 MTR_4g104370 54.723 307 128 6 5 303 40 343 6.92e-113 331
MS.gene006646.t1 MTR_2g075830 55.082 305 134 3 1 302 35 339 6.36e-111 325
MS.gene006646.t1 MTR_3g116080 52.318 302 138 3 5 302 47 346 1.62e-108 319
MS.gene006646.t1 MTR_4g094918 51.299 308 140 5 5 303 35 341 3.62e-108 318
MS.gene006646.t1 MTR_4g080360 49.677 310 148 5 1 303 33 341 2.36e-107 316
MS.gene006646.t1 MTR_2g083930 48.408 314 152 3 1 304 31 344 3.13e-107 316
MS.gene006646.t1 MTR_3g102220 50.000 314 145 4 1 303 49 361 6.10e-107 319
MS.gene006646.t1 MTR_1g018840 50.000 310 141 6 5 303 55 361 1.10e-102 308
MS.gene006646.t1 MTR_1g018840 50.000 310 141 6 5 303 55 361 1.25e-102 308
MS.gene006646.t1 MTR_3g112430 49.026 308 148 5 3 302 34 340 1.18e-100 302
MS.gene006646.t1 MTR_7g078600 49.511 307 146 7 1 304 38 338 1.08e-95 286
MS.gene006646.t1 MTR_7g079230 47.712 306 150 6 1 302 35 334 8.43e-91 274
MS.gene006646.t1 MTR_2g016180 43.322 307 162 5 5 302 51 354 2.98e-85 264
MS.gene006646.t1 MTR_2g016270 43.750 304 150 6 5 302 51 339 8.54e-84 260
MS.gene006646.t1 MTR_2g015850 45.361 291 148 6 21 304 78 364 1.99e-81 251
MS.gene006646.t1 MTR_2g015920 40.968 310 168 7 5 303 53 358 3.72e-73 229
MS.gene006646.t1 MTR_4g065077 41.534 313 162 11 5 303 51 356 7.34e-71 223
MS.gene006646.t1 MTR_4g125300 41.311 305 168 6 5 304 51 349 3.24e-69 219
MS.gene006646.t1 MTR_2g067900 41.905 315 160 9 4 301 15 323 1.22e-66 211
MS.gene006646.t1 MTR_1g023210 37.658 316 168 10 5 300 51 357 6.54e-66 211
MS.gene006646.t1 MTR_8g086470 39.482 309 158 10 5 303 67 356 1.40e-64 207
MS.gene006646.t1 MTR_2g067560 43.125 320 149 13 4 299 15 325 1.22e-63 216
MS.gene006646.t1 MTR_4g071060 36.741 313 174 8 5 300 48 353 2.47e-63 204
MS.gene006646.t1 MTR_2g064475 39.884 346 148 14 4 301 15 348 1.52e-62 202
MS.gene006646.t1 MTR_2g015930 38.019 313 174 8 5 303 52 358 9.93e-61 197
MS.gene006646.t1 MTR_1g094135 36.086 327 173 11 2 304 53 367 1.59e-59 195
MS.gene006646.t1 MTR_7g078630 40.370 270 124 6 1 259 702 945 6.90e-56 194
MS.gene006646.t1 MTR_2g066980 37.730 326 141 12 3 304 32 319 8.87e-55 181
MS.gene006646.t1 MTR_1g023810 41.743 218 119 5 87 301 49 261 2.32e-53 175
MS.gene006646.t1 MTR_4g072790 33.775 302 164 8 4 300 47 317 1.33e-47 162
MS.gene006646.t1 MTR_4g073660 33.007 306 165 8 4 300 46 320 1.30e-45 157
MS.gene006646.t1 MTR_4g072340 33.007 306 165 8 4 300 47 321 1.56e-45 157
MS.gene006646.t1 MTR_2g015840 32.667 300 136 11 8 304 36 272 3.08e-36 131
MS.gene006646.t1 MTR_7g079130 60.870 92 35 1 213 303 6 97 1.08e-32 116
MS.gene006646.t1 MTR_7g078580 55.556 99 42 2 206 303 4 101 2.37e-31 114
MS.gene006646.t1 MTR_1g025450 36.994 173 86 4 87 256 55 207 2.86e-30 120
MS.gene006646.t1 MTR_2g016380 42.478 113 61 2 193 302 23 134 1.09e-24 100
MS.gene006646.t1 MTR_7g078310 40.411 146 69 4 43 180 112 247 2.24e-24 99.8
MS.gene006646.t1 MTR_8g087510 41.912 136 72 5 128 261 23 153 2.30e-23 94.7
MS.gene006646.t1 MTR_3g029560 27.237 257 152 8 61 304 4 238 1.17e-22 94.4
MS.gene006646.t1 MTR_7g111060 28.205 273 161 10 47 295 69 330 9.02e-20 88.6
MS.gene006646.t1 MTR_7g111050 27.972 286 163 13 50 302 61 336 1.08e-19 88.6
MS.gene006646.t1 MTR_2g047960 51.250 80 38 1 96 175 7 85 1.19e-18 79.7
MS.gene006646.t1 MTR_7g079260 50.000 72 19 2 230 300 7 62 3.89e-15 70.5
MS.gene006646.t1 MTR_3g082370 28.636 220 129 9 92 294 5 213 1.75e-14 72.0
MS.gene006646.t1 MTR_7g111050 26.316 247 145 11 50 265 56 296 2.09e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006646.t1 AT1G06260 59.672 305 120 3 1 303 39 342 3.22e-130 374
MS.gene006646.t1 AT3G48340 56.129 310 121 6 5 303 38 343 2.07e-118 345
MS.gene006646.t1 AT5G43060 53.398 309 137 4 1 303 46 353 4.10e-114 337
MS.gene006646.t1 AT5G50260 54.369 309 127 5 5 303 38 342 1.17e-113 333
MS.gene006646.t1 AT5G45890 51.746 315 139 5 1 304 34 346 3.12e-113 331
MS.gene006646.t1 AT1G47128 52.303 304 139 5 5 303 50 352 3.81e-111 330
MS.gene006646.t1 AT4G35350 52.288 306 141 2 1 302 47 351 2.73e-110 324
MS.gene006646.t1 AT3G48350 52.412 311 133 6 3 302 36 342 3.23e-109 322
MS.gene006646.t1 AT1G20850 50.987 304 141 3 5 302 51 352 1.29e-105 312
MS.gene006646.t1 AT4G11310 50.327 306 143 5 5 303 49 352 5.92e-104 308
MS.gene006646.t1 AT4G11320 50.654 306 142 6 5 303 56 359 3.01e-103 306
MS.gene006646.t1 AT4G11320 50.654 306 142 6 5 303 56 359 3.01e-103 306
MS.gene006646.t1 AT4G36880 50.482 311 138 6 8 303 52 361 3.18e-102 304
MS.gene006646.t1 AT3G19390 48.039 306 153 4 2 302 40 344 5.91e-102 306
MS.gene006646.t1 AT4G23520 48.852 305 148 7 5 303 47 349 1.95e-101 301
MS.gene006646.t1 AT2G27420 49.371 318 141 7 3 303 33 347 3.37e-101 300
MS.gene006646.t1 AT3G19400 47.403 308 156 4 1 302 40 347 9.18e-101 300
MS.gene006646.t1 AT3G49340 49.521 313 141 8 3 303 33 340 1.26e-100 299
MS.gene006646.t1 AT1G09850 47.883 307 155 3 1 303 28 333 3.11e-99 298
MS.gene006646.t1 AT2G34080 46.326 313 155 6 1 303 35 344 2.04e-93 281
MS.gene006646.t1 AT1G29080 46.278 309 148 8 8 303 42 345 7.44e-88 266
MS.gene006646.t1 AT1G29090 45.338 311 149 8 8 303 50 354 2.00e-85 261
MS.gene006646.t1 AT4G35350 50.000 242 115 3 1 238 47 286 6.96e-79 241
MS.gene006646.t1 AT3G43960 43.182 308 164 8 5 303 41 346 7.57e-79 244
MS.gene006646.t1 AT1G29110 41.694 307 154 6 8 303 41 333 2.14e-75 234
MS.gene006646.t1 AT3G48350 47.431 253 119 5 3 245 36 284 2.84e-75 233
MS.gene006646.t1 AT5G60360 41.639 305 167 5 5 304 59 357 8.46e-73 228
MS.gene006646.t1 AT5G60360 41.311 305 167 6 5 304 59 356 2.45e-70 222
MS.gene006646.t1 AT3G19400 44.939 247 130 4 1 241 40 286 1.76e-69 218
MS.gene006646.t1 AT3G45310 39.344 305 174 6 5 304 59 357 2.59e-69 219
MS.gene006646.t1 AT5G60360 40.678 295 164 5 5 294 59 347 1.71e-67 215
MS.gene006646.t1 AT3G45310 39.016 305 174 7 5 304 59 356 8.49e-67 213
MS.gene006646.t1 AT4G39090 38.006 321 160 10 5 300 51 357 3.92e-66 211
MS.gene006646.t1 AT2G21430 36.246 309 172 8 5 295 48 349 3.27e-61 199
MS.gene006646.t1 AT3G54940 36.677 319 175 8 2 300 48 359 3.24e-58 191
MS.gene006646.t1 AT4G16190 36.190 315 176 8 5 300 55 363 1.12e-56 187
MS.gene006646.t1 AT3G54940 35.652 230 136 3 2 220 48 276 7.93e-41 143
MS.gene006646.t1 AT1G02305 28.671 286 156 12 43 295 63 333 5.48e-21 92.0
MS.gene006646.t1 AT4G01610 27.174 276 162 10 50 295 64 330 9.05e-21 91.7
MS.gene006646.t1 AT4G01610 26.812 276 163 10 50 295 64 330 1.51e-19 88.2
MS.gene006646.t1 AT1G02300 28.910 211 123 7 106 295 146 350 6.08e-15 75.1

Find 70 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTCCAATCCTTGCTTTC+TGG 0.072633 4.1:+26825384 None:intergenic
AGAGTTTCAAAGTACTTATA+TGG 0.243800 4.1:-26825469 MS.gene006646:CDS
ACCTTCAAACCTCTTCTTCA+TGG 0.288966 4.1:+26825647 None:intergenic
CTTATATGGGTCTTGGTACT+AGG 0.296317 4.1:-26825455 MS.gene006646:CDS
GACGAACGGGAAGTCCGTTT+TGG 0.317728 4.1:-26825588 MS.gene006646:CDS
TTTATTGTCTGTGAGATTGT+AGG 0.348908 4.1:+26825512 None:intergenic
AGGTTGCCAAGGTGGCTTAA+TGG 0.360502 4.1:-26824960 MS.gene006646:CDS
GGTGCTGACTGGGGTGAATC+TGG 0.360846 4.1:-26824596 MS.gene006646:CDS
GGCATCAACAAAATAAAATC+AGG 0.363474 4.1:-26825043 MS.gene006646:CDS
AGAATTCATGGGGTGCTGAC+TGG 0.387691 4.1:-26824607 MS.gene006646:CDS
GCTGCAGTGGCAGCTGTAGA+AGG 0.389218 4.1:-26825064 MS.gene006646:CDS
ATTGGATTGTGAAGAATTCA+TGG 0.392004 4.1:-26824619 MS.gene006646:CDS
CAAAGTACTTATATGGGTCT+TGG 0.395625 4.1:-26825462 MS.gene006646:CDS
GGATTCAGGTATGATGAAAA+TGG 0.398407 4.1:-26825414 MS.gene006646:CDS
TGTTCACACAGGAGGTTGTT+GGG 0.402344 4.1:-26825092 MS.gene006646:intron
AAGGAAATATTAATAAGTAT+TGG 0.416014 4.1:-26824637 MS.gene006646:CDS
GTTCACATAATACAGGATTC+AGG 0.420869 4.1:-26825428 MS.gene006646:CDS
GAATTCATGGGGTGCTGACT+GGG 0.423926 4.1:-26824606 MS.gene006646:CDS
GAAGAAGAGGTTTGAAGGTT+GGG 0.426580 4.1:-26825643 MS.gene006646:CDS
GGTGTAACCGTAGTTGGATA+TGG 0.429893 4.1:-26824662 MS.gene006646:CDS
GAGTTTCAAAGTACTTATAT+GGG 0.441325 4.1:-26825468 MS.gene006646:CDS
CATTATGCTGCGAGTATAAG+TGG 0.447078 4.1:-26824836 MS.gene006646:CDS
TATCCTTATGAAGGTGTAGA+TGG 0.448214 4.1:-26824884 MS.gene006646:CDS
AGCTTAGCCTCATTATCAGC+AGG 0.460600 4.1:+26824800 None:intergenic
AATCATGGTGTAACCGTAGT+TGG 0.476071 4.1:-26824668 MS.gene006646:CDS
ATACAAACATAATGACGAAC+GGG 0.476584 4.1:-26825601 MS.gene006646:CDS
TTATATGGGTCTTGGTACTA+GGG 0.478572 4.1:-26825454 MS.gene006646:CDS
TTAAGCCACCTTGGCAACCT+TGG 0.486289 4.1:+26824963 None:intergenic
GCCATGAAGAAGAGGTTTGA+AGG 0.488543 4.1:-26825648 MS.gene006646:CDS
TTGCTTGGTAAATGCCAAAA+CGG 0.493062 4.1:+26825574 None:intergenic
TGAAGAAGAGGTTTGAAGGT+TGG 0.494459 4.1:-26825644 MS.gene006646:CDS
GGGGTGAATCTGGTTATATC+AGG 0.495176 4.1:-26824586 MS.gene006646:CDS
GTGTTCACACAGGAGGTTGT+TGG 0.498927 4.1:-26825093 MS.gene006646:intron
TCCTTCTCCATATCCAACTA+CGG 0.504152 4.1:+26824655 None:intergenic
CGCGACACTTTGAGTAAAGA+AGG 0.504741 4.1:-26824557 MS.gene006646:CDS
AATAAGCTAAACTACACTAG+AGG 0.504746 4.1:+26824503 None:intergenic
ATCTTCCAGAAAGCAAGGAT+TGG 0.515492 4.1:-26825389 MS.gene006646:CDS
TCATTTCAGCTTTATTCAGA+AGG 0.521702 4.1:-26824722 MS.gene006646:CDS
AAACTACACTAGAGGGTAGC+TGG 0.525707 4.1:+26824511 None:intergenic
GTACCATCTACACCTTCATA+AGG 0.526767 4.1:+26824881 None:intergenic
TTCCAGAAAGCAAGGATTGG+AGG 0.532858 4.1:-26825386 MS.gene006646:CDS
GTAACTGAAATCAAGAATCA+AGG 0.536484 4.1:-26825351 MS.gene006646:CDS
TGTGGAAAGCAACTCAATCA+TGG 0.536875 4.1:-26824683 MS.gene006646:CDS
TGCATCAATTGCAACAGACA+CGG 0.539151 4.1:+26824754 None:intergenic
GTATGTATTGAACATTTGCT+TGG 0.546306 4.1:+26825559 None:intergenic
AGCAAGGATTGGAGGAAGGA+GGG 0.547902 4.1:-26825378 MS.gene006646:CDS
AGGGTGCGTTCACATAATAC+AGG 0.550141 4.1:-26825435 MS.gene006646:CDS
TTGTTGGGCATTCGCTGCAG+TGG 0.553618 4.1:-26825077 MS.gene006646:CDS
TGGTGATCTTCCAGAAAGCA+AGG 0.571198 4.1:-26825394 MS.gene006646:CDS
ACCGTAGTTGGATATGGAGA+AGG 0.573204 4.1:-26824656 MS.gene006646:CDS
AGAAGTTCCTGCTGATAATG+AGG 0.576412 4.1:-26824807 MS.gene006646:CDS
AATGGTAACCAAGGTTGCCA+AGG 0.576933 4.1:-26824971 MS.gene006646:CDS
AAGCAAGGATTGGAGGAAGG+AGG 0.583928 4.1:-26825379 MS.gene006646:CDS
GTGTCTGTTGCAATTGATGC+AGG 0.584191 4.1:-26824752 MS.gene006646:CDS
AATACAAACATAATGACGAA+CGG 0.584501 4.1:-26825602 MS.gene006646:CDS
CTGTCTCCATTAAGCCACCT+TGG 0.587016 4.1:+26824954 None:intergenic
ACGTAGAAGCCATGAAGAAG+AGG 0.591122 4.1:-26825656 None:intergenic
GAAGGTTGGGTGAAACGACA+TGG 0.591844 4.1:-26825630 MS.gene006646:CDS
GCATCAATTGCAACAGACAC+GGG 0.598881 4.1:+26824755 None:intergenic
GGTAACCAAGGTTGCCAAGG+TGG 0.606188 4.1:-26824968 MS.gene006646:CDS
ATAAGCTAAACTACACTAGA+GGG 0.610529 4.1:+26824504 None:intergenic
TTGGATTGTGAAGAATTCAT+GGG 0.612300 4.1:-26824618 MS.gene006646:CDS
AGAAAGCAAGGATTGGAGGA+AGG 0.613399 4.1:-26825382 MS.gene006646:CDS
GCATCAACAAAATAAAATCA+GGG 0.617083 4.1:-26825042 MS.gene006646:CDS
AATTCATGGGGTGCTGACTG+GGG 0.644464 4.1:-26824605 MS.gene006646:CDS
TTGAGTAAAGAAGGTATGTG+TGG 0.649760 4.1:-26824548 MS.gene006646:CDS
TACAAGAAACATTACCACAC+TGG 0.663674 4.1:+26825328 None:intergenic
ATCAAGAATCAAGGCCAGTG+TGG 0.700928 4.1:-26825342 MS.gene006646:intron
TATACTCGCAGCATAATGTG+CGG 0.723834 4.1:+26824841 None:intergenic
TGGATTGTGAAGAATTCATG+GGG 0.729679 4.1:-26824617 MS.gene006646:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGGAAATATTAATAAGTAT+TGG - chr4.1:26825522-26825541 MS.gene006646:CDS 15.0%
!! ATATCAAGTTAAAAAAACAT+TGG + chr4.1:26824976-26824995 None:intergenic 15.0%
!! TATACATTCATCATAAAAAA+TGG - chr4.1:26825230-26825249 MS.gene006646:intron 15.0%
!! ATAGATTGTGATGTTAAAAA+TGG - chr4.1:26825170-26825189 MS.gene006646:intron 20.0%
! AATACAAACATAATGACGAA+CGG - chr4.1:26824557-26824576 MS.gene006646:CDS 25.0%
! ACATTCATCATAAAAAATGG+CGG - chr4.1:26825233-26825252 MS.gene006646:intron 25.0%
! ATGTGATTTCTTACAACTTA+AGG - chr4.1:26824935-26824954 MS.gene006646:CDS 25.0%
! GCATCAACAAAATAAAATCA+GGG - chr4.1:26825117-26825136 MS.gene006646:intron 25.0%
!! AGAGTTTCAAAGTACTTATA+TGG - chr4.1:26824690-26824709 MS.gene006646:CDS 25.0%
!! GAGTTTCAAAGTACTTATAT+GGG - chr4.1:26824691-26824710 MS.gene006646:CDS 25.0%
!! TTCATAAGGATAGTCTTTTT+CGG + chr4.1:26825267-26825286 None:intergenic 25.0%
!!! AACATTGGTATTTTAACTTG+TGG - chr4.1:26825003-26825022 MS.gene006646:CDS 25.0%
ATACAAACATAATGACGAAC+GGG - chr4.1:26824558-26824577 MS.gene006646:CDS 30.0%
ATAGCTTGAATCGCATAATA+TGG + chr4.1:26824908-26824927 None:intergenic 30.0%
ATTGGATTGTGAAGAATTCA+TGG - chr4.1:26825540-26825559 MS.gene006646:CDS 30.0%
CTTGATATTGTACGAAACAT+TGG - chr4.1:26824988-26825007 MS.gene006646:CDS 30.0%
GAAAAAGACTATCCTTATGA+AGG - chr4.1:26825266-26825285 MS.gene006646:intron 30.0%
GATGTTAAAAATGGTAACCA+AGG - chr4.1:26825179-26825198 MS.gene006646:intron 30.0%
GGCATCAACAAAATAAAATC+AGG - chr4.1:26825116-26825135 MS.gene006646:intron 30.0%
GTAACTGAAATCAAGAATCA+AGG - chr4.1:26824808-26824827 MS.gene006646:CDS 30.0%
GTATGTATTGAACATTTGCT+TGG + chr4.1:26824603-26824622 None:intergenic 30.0%
TTGGATTGTGAAGAATTCAT+GGG - chr4.1:26825541-26825560 MS.gene006646:CDS 30.0%
TTTATTGTCTGTGAGATTGT+AGG + chr4.1:26824650-26824669 None:intergenic 30.0%
TTTCTTGTAACAGAATTGCA+TGG - chr4.1:26824845-26824864 MS.gene006646:CDS 30.0%
!! TCATTTCAGCTTTATTCAGA+AGG - chr4.1:26825437-26825456 MS.gene006646:CDS 30.0%
GGATTCAGGTATGATGAAAA+TGG - chr4.1:26824745-26824764 MS.gene006646:CDS 35.0%
GTTCACATAATACAGGATTC+AGG - chr4.1:26824731-26824750 MS.gene006646:CDS 35.0%
TACAAGAAACATTACCACAC+TGG + chr4.1:26824834-26824853 None:intergenic 35.0%
TGGATTGTGAAGAATTCATG+GGG - chr4.1:26825542-26825561 MS.gene006646:CDS 35.0%
TTATATGGGTCTTGGTACTA+GGG - chr4.1:26824705-26824724 MS.gene006646:CDS 35.0%
TTGCTTGGTAAATGCCAAAA+CGG + chr4.1:26824588-26824607 None:intergenic 35.0%
! AGAATTGCATGGCTTTTTAG+AGG - chr4.1:26824856-26824875 MS.gene006646:CDS 35.0%
! CAAAGTACTTATATGGGTCT+TGG - chr4.1:26824697-26824716 MS.gene006646:CDS 35.0%
! TATCCTTATGAAGGTGTAGA+TGG - chr4.1:26825275-26825294 MS.gene006646:intron 35.0%
! TTGAGTAAAGAAGGTATGTG+TGG - chr4.1:26825611-26825630 MS.gene006646:CDS 35.0%
!!! ATAAGGATAGTCTTTTTCGG+TGG + chr4.1:26825264-26825283 None:intergenic 35.0%
!!! CTTTTAAGTTGTGTTCACAC+AGG - chr4.1:26825056-26825075 MS.gene006646:CDS 35.0%
!!! TATTCAGAAGGTGTTTTCTC+TGG - chr4.1:26825449-26825468 MS.gene006646:CDS 35.0%
AATCATGGTGTAACCGTAGT+TGG - chr4.1:26825491-26825510 MS.gene006646:CDS 40.0%
ATCTTCCAGAAAGCAAGGAT+TGG - chr4.1:26824770-26824789 MS.gene006646:CDS 40.0%
CATTATGCTGCGAGTATAAG+TGG - chr4.1:26825323-26825342 MS.gene006646:intron 40.0%
CTTATATGGGTCTTGGTACT+AGG - chr4.1:26824704-26824723 MS.gene006646:CDS 40.0%
GAAGAAGAGGTTTGAAGGTT+GGG - chr4.1:26824516-26824535 MS.gene006646:CDS 40.0%
GTACCATCTACACCTTCATA+AGG + chr4.1:26825281-26825300 None:intergenic 40.0%
TATACTCGCAGCATAATGTG+CGG + chr4.1:26825321-26825340 None:intergenic 40.0%
TCCTTCTCCATATCCAACTA+CGG + chr4.1:26825507-26825526 None:intergenic 40.0%
TGAAGAAGAGGTTTGAAGGT+TGG - chr4.1:26824515-26824534 MS.gene006646:CDS 40.0%
TGCATCAATTGCAACAGACA+CGG + chr4.1:26825408-26825427 None:intergenic 40.0%
TGTGGAAAGCAACTCAATCA+TGG - chr4.1:26825476-26825495 MS.gene006646:CDS 40.0%
TGTGGAACATTCTCTCAAGA+TGG - chr4.1:26825021-26825040 MS.gene006646:CDS 40.0%
! AGAAGTTCCTGCTGATAATG+AGG - chr4.1:26825352-26825371 MS.gene006646:CDS 40.0%
! AGCATAATGTGCGGCTTTTT+CGG + chr4.1:26825312-26825331 None:intergenic 40.0%
! TTAAGTTGTGTTCACACAGG+AGG - chr4.1:26825059-26825078 MS.gene006646:CDS 40.0%
!! CATGGCTTTTTAGAGGTTCT+AGG - chr4.1:26824863-26824882 MS.gene006646:CDS 40.0%
AATGGTAACCAAGGTTGCCA+AGG - chr4.1:26825188-26825207 MS.gene006646:intron 45.0%
ACCGTAGTTGGATATGGAGA+AGG - chr4.1:26825503-26825522 MS.gene006646:CDS 45.0%
AGAAAGCAAGGATTGGAGGA+AGG - chr4.1:26824777-26824796 MS.gene006646:CDS 45.0%
AGCTTAGCCTCATTATCAGC+AGG + chr4.1:26825362-26825381 None:intergenic 45.0%
AGGGTGCGTTCACATAATAC+AGG - chr4.1:26824724-26824743 MS.gene006646:CDS 45.0%
ATCAAGAATCAAGGCCAGTG+TGG - chr4.1:26824817-26824836 MS.gene006646:CDS 45.0%
CGCGACACTTTGAGTAAAGA+AGG - chr4.1:26825602-26825621 MS.gene006646:CDS 45.0%
GCATCAATTGCAACAGACAC+GGG + chr4.1:26825407-26825426 None:intergenic 45.0%
GGGGTGAATCTGGTTATATC+AGG - chr4.1:26825573-26825592 MS.gene006646:CDS 45.0%
GGTGTAACCGTAGTTGGATA+TGG - chr4.1:26825497-26825516 MS.gene006646:CDS 45.0%
GTGTCTGTTGCAATTGATGC+AGG - chr4.1:26825407-26825426 MS.gene006646:CDS 45.0%
TGGTGATCTTCCAGAAAGCA+AGG - chr4.1:26824765-26824784 MS.gene006646:CDS 45.0%
TGTTCACACAGGAGGTTGTT+GGG - chr4.1:26825067-26825086 MS.gene006646:CDS 45.0%
TTCCAGAAAGCAAGGATTGG+AGG - chr4.1:26824773-26824792 MS.gene006646:CDS 45.0%
TTCCTCCAATCCTTGCTTTC+TGG + chr4.1:26824778-26824797 None:intergenic 45.0%
! GGTGTTTTCTCTGGCATTTG+TGG - chr4.1:26825458-26825477 MS.gene006646:CDS 45.0%
AAGCAAGGATTGGAGGAAGG+AGG - chr4.1:26824780-26824799 MS.gene006646:CDS 50.0%
AGCAAGGATTGGAGGAAGGA+GGG - chr4.1:26824781-26824800 MS.gene006646:CDS 50.0%
AGGTTGCCAAGGTGGCTTAA+TGG - chr4.1:26825199-26825218 MS.gene006646:intron 50.0%
CTGTCTCCATTAAGCCACCT+TGG + chr4.1:26825208-26825227 None:intergenic 50.0%
GAAGGTTGGGTGAAACGACA+TGG - chr4.1:26824529-26824548 MS.gene006646:CDS 50.0%
GTGTTCACACAGGAGGTTGT+TGG - chr4.1:26825066-26825085 MS.gene006646:CDS 50.0%
TTAAGCCACCTTGGCAACCT+TGG + chr4.1:26825199-26825218 None:intergenic 50.0%
!! AATTCATGGGGTGCTGACTG+GGG - chr4.1:26825554-26825573 MS.gene006646:CDS 50.0%
!! AGAATTCATGGGGTGCTGAC+TGG - chr4.1:26825552-26825571 MS.gene006646:CDS 50.0%
!! GAATTCATGGGGTGCTGACT+GGG - chr4.1:26825553-26825572 MS.gene006646:CDS 50.0%
!!! TGCGGCTTTTTCGGTCTTAC+AGG + chr4.1:26825303-26825322 None:intergenic 50.0%
GGTAACCAAGGTTGCCAAGG+TGG - chr4.1:26825191-26825210 MS.gene006646:intron 55.0%
TTGTTGGGCATTCGCTGCAG+TGG - chr4.1:26825082-26825101 MS.gene006646:CDS 55.0%
! GACGAACGGGAAGTCCGTTT+TGG - chr4.1:26824571-26824590 MS.gene006646:CDS 55.0%
GCTGCAGTGGCAGCTGTAGA+AGG - chr4.1:26825095-26825114 MS.gene006646:intron 60.0%
!! GGTGCTGACTGGGGTGAATC+TGG - chr4.1:26825563-26825582 MS.gene006646:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 26824514 26825667 26824514 ID=MS.gene006646
chr4.1 mRNA 26824514 26825667 26824514 ID=MS.gene006646.t1;Parent=MS.gene006646
chr4.1 exon 26825343 26825667 26825343 ID=MS.gene006646.t1.exon1;Parent=MS.gene006646.t1
chr4.1 CDS 26825343 26825667 26825343 ID=cds.MS.gene006646.t1;Parent=MS.gene006646.t1
chr4.1 exon 26824514 26825103 26824514 ID=MS.gene006646.t1.exon2;Parent=MS.gene006646.t1
chr4.1 CDS 26824514 26825103 26824514 ID=cds.MS.gene006646.t1;Parent=MS.gene006646.t1
Gene Sequence

>MS.gene006646

ATGAAGAAGAGGTTTGAAGGTTGGGTGAAACGACATGGTCGAAAATACAAACATAATGACGAACGGGAAGTCCGTTTTGGCATTTACCAAGCAAATGTTCAATACATACATTGCAAAAACGCACAGAAAAACTCCTACAATCTCACAGACAATAAATTTGCAGATCTTACGAATGAAGAGTTTCAAAGTACTTATATGGGTCTTGGTACTAGGGTGCGTTCACATAATACAGGATTCAGGTATGATGAAAATGGTGATCTTCCAGAAAGCAAGGATTGGAGGAAGGAGGGAGCAGTAACTGAAATCAAGAATCAAGGCCAGTGTGGTAATGTTTCTTGTAACAGAATTGCATGGCTTTTTAGAGGTTCTAGGTTATGCTATTTCAGTTTTACCATATTATGCGATTCAAGCTATGTTACTTATGTGATTTCTTACAACTTAAGGAACATTCTTACTCTGCCAATGTTTTTTTAACTTGATATTGTACGAAACATTGGTATTTTAACTTGTGGAACATTCTCTCAAGATGGTTGTTTTAACTACTTTTAAGTTGTGTTCACACAGGAGGTTGTTGGGCATTCGCTGCAGTGGCAGCTGTAGAAGGCATCAACAAAATAAAATCAGGGAAATTGATCTCTCTATCTGAACAAGAACTAATAGATTGTGATGTTAAAAATGGTAACCAAGGTTGCCAAGGTGGCTTAATGGAGACAGCATATACATTCATCATAAAAAATGGCGGACTAACCACCGAAAAAGACTATCCTTATGAAGGTGTAGATGGTACCTGTAAGACCGAAAAAGCCGCACATTATGCTGCGAGTATAAGTGGATATGAAGAAGTTCCTGCTGATAATGAGGCTAAGCTTAAAGCTGCTGCAGCTCATCAACCCGTGTCTGTTGCAATTGATGCAGGAAGTTATTCATTTCAGCTTTATTCAGAAGGTGTTTTCTCTGGCATTTGTGGAAAGCAACTCAATCATGGTGTAACCGTAGTTGGATATGGAGAAGGAAATATTAATAAGTATTGGATTGTGAAGAATTCATGGGGTGCTGACTGGGGTGAATCTGGTTATATCAGGATGAAGCGCGACACTTTGAGTAAAGAAGGTATGTGTGGCATTGCTATGCAAGCCAGCTACCCTCTAGTGTAG
Protein sequence

>MS.gene006646.t1

MKKRFEGWVKRHGRKYKHNDEREVRFGIYQANVQYIHCKNAQKNSYNLTDNKFADLTNEEFQSTYMGLGTRVRSHNTGFRYDENGDLPESKDWRKEGAVTEIKNQGQCGGCWAFAAVAAVEGINKIKSGKLISLSEQELIDCDVKNGNQGCQGGLMETAYTFIIKNGGLTTEKDYPYEGVDGTCKTEKAAHYAASISGYEEVPADNEAKLKAAAAHQPVSVAIDAGSYSFQLYSEGVFSGICGKQLNHGVTVVGYGEGNINKYWIVKNSWGADWGESGYIRMKRDTLSKEGMCGIAMQASYPLV