AlfalfaGEDB | MS.gene23321

M. sativa cultivar XinJiangDaYe / MS.gene23321

KEGG: K01373
GO: GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00112.26 PF08246.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23321.t1	XP_003606998.1	92.3	362	8	1	1	342	1	362	1.00E-194	689.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23321.t1	P43295	69.4	359	87	3	1	337	1	358	1.1e-144	514.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23321.t1	G7JIL2	92.3	362	8	1	1	342	1	362	7.5e-195	689.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054004	MS.gene23321	0.808335	3.26E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene23321	MS.gene045458	PPI
MS.gene23321	MS.gene026874	PPI
MS.gene23321	MS.gene73815	PPI
MS.gene23321	MS.gene95543	PPI
MS.gene23321	MS.gene006720	PPI
MS.gene23321	MS.gene045501	PPI
MS.gene23321	MS.gene012206	PPI
MS.gene23321	MS.gene055760	PPI
MS.gene23321	MS.gene052630	PPI
MS.gene23321	MS.gene045525	PPI
MS.gene23321	MS.gene50738	PPI
MS.gene23321	MS.gene006665	PPI
MS.gene23321	MS.gene006680	PPI
MS.gene66833	MS.gene23321	PPI
MS.gene23321	MS.gene069978	PPI
MS.gene23321	MS.gene69195	PPI
MS.gene23321	MS.gene003680	PPI
MS.gene23321	MS.gene003677	PPI
MS.gene23321	MS.gene26670	PPI
MS.gene23321	MS.gene63719	PPI
MS.gene23321	MS.gene005934	PPI
MS.gene23321	MS.gene26669	PPI
MS.gene23321	MS.gene78715	PPI
MS.gene23321	MS.gene059966	PPI
MS.gene23321	MS.gene59493	PPI
MS.gene23321	MS.gene73817	PPI
MS.gene23321	MS.gene011363	PPI
MS.gene23321	MS.gene63396	PPI
MS.gene23321	MS.gene052628	PPI
MS.gene23321	MS.gene34090	PPI
MS.gene23321	MS.gene64795	PPI
MS.gene23321	MS.gene73834	PPI
MS.gene23321	MS.gene73835	PPI
MS.gene23321	MS.gene005933	PPI
MS.gene23321	MS.gene064873	PPI
MS.gene006646	MS.gene23321	PPI
MS.gene23321	MS.gene045461	PPI
MS.gene23321	MS.gene026873	PPI
MS.gene23321	MS.gene065787	PPI
MS.gene23321	MS.gene033324	PPI
MS.gene23321	MS.gene09529	PPI
MS.gene028850	MS.gene23321	PPI
MS.gene23321	MS.gene033151	PPI
MS.gene23321	MS.gene045455	PPI
MS.gene23321	MS.gene24818	PPI
MS.gene23321	MS.gene006682	PPI
MS.gene23321	MS.gene031638	PPI
MS.gene23321	MS.gene26853	PPI
MS.gene23321	MS.gene045526	PPI
MS.gene23321	MS.gene045520	PPI
MS.gene014304	MS.gene23321	PPI
MS.gene23321	MS.gene67468	PPI
MS.gene23321	MS.gene73838	PPI
MS.gene23321	MS.gene045503	PPI
MS.gene23321	MS.gene043473	PPI
MS.gene23321	MS.gene031581	PPI
MS.gene23321	MS.gene29082	PPI
MS.gene23321	MS.gene48296	PPI
MS.gene23321	MS.gene73840	PPI
MS.gene23321	MS.gene065785	PPI
MS.gene23321	MS.gene027420	PPI
MS.gene23321	MS.gene031580	PPI
MS.gene23321	MS.gene045522	PPI
MS.gene23321	MS.gene25255	PPI
MS.gene23321	MS.gene031635	PPI
MS.gene23321	MS.gene022410	PPI
MS.gene23321	MS.gene87639	PPI
MS.gene23321	MS.gene060218	PPI
MS.gene23321	MS.gene031619	PPI
MS.gene23321	MS.gene031621	PPI
MS.gene23321	MS.gene045459	PPI
MS.gene23321	MS.gene026876	PPI
MS.gene23321	MS.gene51064	PPI
MS.gene23321	MS.gene058156	PPI
MS.gene23321	MS.gene31410	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23321.t1	MTR_4g071060	92.265	362	8	1	1	342	1	362	0.0	694
MS.gene23321.t1	MTR_1g023210	66.576	368	91	6	1	340	1	364	3.28e-177	496
MS.gene23321.t1	MTR_4g073660	63.912	363	73	7	3	343	4	330	1.23e-160	452
MS.gene23321.t1	MTR_4g072790	64.560	364	65	8	3	343	5	327	2.84e-160	451
MS.gene23321.t1	MTR_4g072340	63.488	367	74	8	1	343	1	331	7.93e-160	450
MS.gene23321.t1	MTR_1g094135	51.821	357	145	4	10	340	15	370	1.17e-133	386
MS.gene23321.t1	MTR_4g079770	38.136	354	160	12	5	328	12	336	4.79e-64	207
MS.gene23321.t1	MTR_4g047610	39.550	311	142	9	54	333	44	339	1.70e-63	205
MS.gene23321.t1	MTR_4g125300	38.202	356	174	11	8	333	6	345	1.70e-63	206
MS.gene23321.t1	MTR_4g079470	40.127	314	142	10	45	328	39	336	4.61e-63	204
MS.gene23321.t1	MTR_4g079800	38.871	319	149	10	45	333	39	341	2.48e-62	202
MS.gene23321.t1	MTR_5g022560	39.062	320	148	10	45	333	37	340	4.85e-62	201
MS.gene23321.t1	MTR_1g107550	41.000	300	131	7	50	321	54	335	8.44e-62	204
MS.gene23321.t1	MTR_4g094918	38.136	354	143	13	5	321	7	321	1.05e-60	199
MS.gene23321.t1	MTR_4g079440	33.521	355	190	10	5	333	7	341	2.08e-60	197
MS.gene23321.t1	MTR_3g102220	35.345	348	172	7	5	321	16	341	8.04e-60	199
MS.gene23321.t1	MTR_3g116080	37.975	316	148	9	48	333	47	344	1.16e-59	196
MS.gene23321.t1	MTR_4g080700	37.107	318	155	9	38	328	32	331	1.42e-59	195
MS.gene23321.t1	MTR_4g107930	38.585	311	145	9	54	333	46	341	2.93e-59	194
MS.gene23321.t1	MTR_4g080730	37.086	302	147	8	54	328	46	331	3.91e-58	191
MS.gene23321.t1	MTR_3g112430	38.006	321	146	11	48	333	36	338	1.62e-57	192
MS.gene23321.t1	MTR_1g018840	36.443	343	166	9	9	321	21	341	3.92e-57	192
MS.gene23321.t1	MTR_1g018840	36.443	343	166	9	9	321	21	341	4.10e-57	192
MS.gene23321.t1	MTR_3g075390	37.458	299	138	9	63	328	54	336	1.15e-55	186
MS.gene23321.t1	MTR_2g075830	37.417	302	148	7	54	328	45	332	1.40e-55	185
MS.gene23321.t1	MTR_4g080360	35.831	307	149	9	54	328	43	333	3.65e-55	184
MS.gene23321.t1	MTR_4g104370	37.500	304	140	9	58	328	49	335	8.07e-55	183
MS.gene23321.t1	MTR_2g090890	34.810	316	166	7	48	339	39	338	7.55e-53	177
MS.gene23321.t1	MTR_2g016270	36.928	306	154	11	48	333	51	337	5.23e-52	179
MS.gene23321.t1	MTR_2g015850	38.028	284	133	7	64	321	78	344	2.93e-51	174
MS.gene23321.t1	MTR_2g016180	35.826	321	152	12	48	333	51	352	4.86e-50	174
MS.gene23321.t1	MTR_4g065077	33.065	372	179	14	4	332	8	352	5.53e-50	171
MS.gene23321.t1	MTR_2g015920	30.601	366	188	11	6	330	12	352	1.19e-49	170
MS.gene23321.t1	MTR_2g083930	35.135	333	163	13	41	338	30	344	7.67e-49	167
MS.gene23321.t1	MTR_7g078600	33.758	314	171	9	37	328	31	329	8.40e-47	162
MS.gene23321.t1	MTR_8g086470	33.046	348	167	12	4	321	24	335	1.09e-46	162
MS.gene23321.t1	MTR_2g015930	30.028	363	193	12	6	329	11	351	3.60e-45	158
MS.gene23321.t1	MTR_8g087510	50.000	136	57	3	152	286	23	148	1.08e-41	143
MS.gene23321.t1	MTR_7g079230	31.488	289	175	6	45	328	57	327	2.33e-41	147
MS.gene23321.t1	MTR_2g067900	33.333	336	157	14	42	333	10	322	3.37e-41	147
MS.gene23321.t1	MTR_1g023810	40.930	215	107	7	116	327	54	251	2.95e-38	137
MS.gene23321.t1	MTR_2g064475	35.772	246	118	11	110	342	134	352	2.15e-36	135
MS.gene23321.t1	MTR_2g067560	32.110	327	149	12	42	321	10	310	2.59e-34	134
MS.gene23321.t1	MTR_2g066980	27.273	374	165	15	7	335	4	315	1.31e-28	113
MS.gene23321.t1	MTR_7g078630	30.315	254	149	6	48	288	706	944	1.62e-26	110
MS.gene23321.t1	MTR_7g111060	27.247	356	206	14	5	333	6	335	2.42e-21	94.0
MS.gene23321.t1	MTR_7g111050	30.800	250	133	10	109	333	98	332	2.06e-20	91.3
MS.gene23321.t1	MTR_1g025450	36.559	186	89	7	104	286	48	207	2.78e-19	89.4
MS.gene23321.t1	MTR_2g047960	42.391	92	45	1	115	206	2	85	8.00e-15	69.7
MS.gene23321.t1	MTR_7g078310	43.299	97	46	2	116	210	157	246	9.69e-15	73.6
MS.gene23321.t1	MTR_2g015840	31.325	166	80	5	29	164	18	179	3.00e-14	72.4
MS.gene23321.t1	MTR_2g015840	34.746	118	69	3	224	340	161	271	2.56e-13	69.7
MS.gene23321.t1	MTR_3g029560	28.821	229	111	11	122	338	49	237	2.13e-12	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23321.t1	AT2G21430	69.167	360	88	3	1	338	1	359	0.0	518
MS.gene23321.t1	AT4G39090	68.067	357	92	2	8	342	10	366	0.0	508
MS.gene23321.t1	AT4G16190	64.171	374	102	5	1	343	1	373	1.80e-169	476
MS.gene23321.t1	AT3G54940	57.064	361	118	7	6	336	9	362	1.16e-145	416
MS.gene23321.t1	AT3G54940	51.636	275	97	6	6	251	9	276	1.84e-91	275
MS.gene23321.t1	AT3G45310	38.873	355	164	12	10	328	11	348	1.77e-65	211
MS.gene23321.t1	AT5G60360	39.157	332	156	10	31	333	39	353	3.60e-65	210
MS.gene23321.t1	AT3G45310	38.527	353	164	12	10	326	11	346	4.41e-64	207
MS.gene23321.t1	AT5G60360	39.571	326	151	10	31	327	39	347	5.59e-64	207
MS.gene23321.t1	AT3G19400	40.000	310	136	9	57	333	53	345	9.82e-64	206
MS.gene23321.t1	AT5G60360	39.385	325	151	10	31	326	39	346	1.25e-63	206
MS.gene23321.t1	AT1G09850	38.700	323	149	10	48	339	32	336	5.52e-62	204
MS.gene23321.t1	AT1G20850	38.486	317	147	8	48	333	51	350	2.13e-61	200
MS.gene23321.t1	AT3G19390	37.396	361	165	13	8	333	8	342	2.41e-61	203
MS.gene23321.t1	AT1G47128	40.203	296	137	6	50	321	54	333	2.64e-61	203
MS.gene23321.t1	AT5G43060	41.489	282	124	8	64	321	70	334	3.05e-59	197
MS.gene23321.t1	AT3G43960	39.932	293	135	7	56	321	49	327	2.46e-58	193
MS.gene23321.t1	AT5G45890	34.435	363	175	11	1	328	3	337	9.44e-58	191
MS.gene23321.t1	AT4G35350	36.709	316	153	9	48	333	51	349	3.71e-57	189
MS.gene23321.t1	AT4G36880	38.408	289	131	6	64	321	69	341	1.01e-56	189
MS.gene23321.t1	AT1G29090	36.088	363	162	14	6	328	14	346	1.03e-56	188
MS.gene23321.t1	AT1G06260	36.923	325	144	13	45	333	40	339	9.59e-56	185
MS.gene23321.t1	AT3G48340	35.920	348	161	10	9	321	4	324	1.16e-55	186
MS.gene23321.t1	AT4G23520	33.518	361	181	14	9	333	9	346	1.39e-54	182
MS.gene23321.t1	AT2G27420	34.810	316	148	10	54	328	41	339	1.55e-53	179
MS.gene23321.t1	AT5G50260	37.171	304	142	8	58	328	47	334	1.64e-52	177
MS.gene23321.t1	AT3G48350	34.870	347	171	10	7	322	3	325	2.03e-52	177
MS.gene23321.t1	AT1G29080	36.164	318	153	9	54	337	45	346	3.63e-52	176
MS.gene23321.t1	AT3G49340	33.546	313	155	10	54	331	41	335	6.10e-50	170
MS.gene23321.t1	AT2G34080	35.370	311	146	11	54	328	45	336	1.36e-49	169
MS.gene23321.t1	AT4G11310	31.476	359	187	10	6	328	9	344	3.16e-48	166
MS.gene23321.t1	AT3G19400	39.556	225	104	4	57	256	53	270	2.52e-47	162
MS.gene23321.t1	AT4G11320	34.286	315	154	9	48	328	56	351	4.73e-47	163
MS.gene23321.t1	AT4G11320	34.286	315	154	9	48	328	56	351	4.73e-47	163
MS.gene23321.t1	AT4G35350	35.622	233	117	5	48	256	51	274	1.02e-41	147
MS.gene23321.t1	AT1G29110	31.511	311	148	9	54	328	44	325	9.38e-41	146
MS.gene23321.t1	AT3G48350	32.781	302	139	9	7	267	3	281	9.93e-37	134
MS.gene23321.t1	AT1G02305	30.400	250	132	11	109	332	104	337	7.07e-20	89.7
MS.gene23321.t1	AT4G01610	28.571	308	165	12	61	329	40	331	8.67e-20	89.4
MS.gene23321.t1	AT4G01610	28.247	308	166	12	61	329	40	331	1.53e-18	85.9
MS.gene23321.t1	AT1G02300	29.778	225	120	11	130	332	146	354	1.65e-17	83.2

Find 83 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCATATATACTGCATTTA+AGG	0.130654	4.4:+37541231	None:intergenic
TCGACCACCTGACGGATTAT+TGG	0.223904	4.4:+37542613	None:intergenic
AACCTATTTAAACATAAGTT+TGG	0.234066	4.4:-37542553	MS.gene23321:CDS
TCCCAAACTTATGTTTAAAT+AGG	0.268902	4.4:+37542551	None:intergenic
GAAGAAGATTATCCTTATTC+TGG	0.284846	4.4:-37541486	MS.gene23321:CDS
CCAAGGATGAACATGATTAC+AGG	0.304516	4.4:-37542516	MS.gene23321:CDS
CTGGAGCCTATTCGCCTATT+AGG	0.320950	4.4:-37541129	MS.gene23321:CDS
GGAGTTTCGTCAGAGCGTTT+TGG	0.321592	4.4:-37542392	MS.gene23321:CDS
GGTAAGACTTGGGGAGAAAA+TGG	0.331018	4.4:-37541067	MS.gene23321:CDS
CCACGATCAGTACCAGAATA+AGG	0.365501	4.4:+37541474	None:intergenic
ACTAGATCATGGAGTGTTAC+TGG	0.371273	4.4:-37541164	MS.gene23321:CDS
ACCTATTTAAACATAAGTTT+GGG	0.393244	4.4:-37542552	MS.gene23321:CDS
AGTCTCTTTGAGCATATGTA+TGG	0.401014	4.4:+37541184	None:intergenic
GGCTTCTCCTTCATCCTAAT+AGG	0.407412	4.4:+37541115	None:intergenic
TCACAAGAACCCGGTTGCTC+TGG	0.408096	4.4:+37541579	None:intergenic
GGAGTGTTACTGGTGGGATA+TGG	0.410428	4.4:-37541154	MS.gene23321:CDS
GGATGAAGGAGAAGCCATAT+TGG	0.423435	4.4:-37541108	MS.gene23321:CDS
TGCTAAGAAGCATCAATTGA+TGG	0.425569	4.4:-37542464	MS.gene23321:CDS
CTTCATCCTAATAGGCGAAT+AGG	0.432818	4.4:+37541123	None:intergenic
TGACAAGATTAGCGGCAATC+TGG	0.437809	4.4:+37541379	None:intergenic
CAACCGGGTTCTTGTGATGC+CGG	0.438520	4.4:-37541573	MS.gene23321:CDS
ATAGTGTGATCCAGAGCAAC+CGG	0.450276	4.4:-37541589	MS.gene23321:intron
CGTCAGGTGGTCGACGAAGA+AGG	0.454316	4.4:-37542604	MS.gene23321:CDS
AAAACTCATGGGGTAAGACT+TGG	0.455227	4.4:-37541078	MS.gene23321:CDS
TCTTGTGATGCCGGGTGCAA+TGG	0.456716	4.4:-37541564	MS.gene23321:CDS
CCTGTAATCATGTTCATCCT+TGG	0.456777	4.4:+37542516	None:intergenic
GCATCAATTGATGGATCCTT+CGG	0.466749	4.4:-37542455	MS.gene23321:CDS
TGTTGGAGTTTCAGTACAAC+CGG	0.469204	4.4:-37542136	MS.gene23321:CDS
ATGGATCCTTCGGCGGTTCA+CGG	0.470073	4.4:-37542445	MS.gene23321:CDS
ATGGATACTACAAAATATGC+AGG	0.476628	4.4:-37541048	MS.gene23321:CDS
AAACTCATGGGGTAAGACTT+GGG	0.490997	4.4:-37541077	MS.gene23321:CDS
AACCGGGTTCTTGTGATGCC+GGG	0.494687	4.4:-37541572	MS.gene23321:CDS
GACGAAGAAGGAGTGCGGTT+GGG	0.502317	4.4:-37542592	MS.gene23321:CDS
ATTGGATCTTCTCCTTCGTC+CGG	0.505970	4.4:+37542631	None:intergenic
TTACTGGTGGGATATGGATC+TGG	0.507620	4.4:-37541148	MS.gene23321:CDS
TCAATTGATGCTTCTTAGCA+CGG	0.511562	4.4:+37542467	None:intergenic
CCTTATTCTGGTACTGATCG+TGG	0.512596	4.4:-37541474	MS.gene23321:CDS
TGGGAAGGTTTATTCTTCCA+AGG	0.519917	4.4:-37542533	MS.gene23321:CDS
AGAAGATCCAATAATCCGTC+AGG	0.520179	4.4:-37542620	MS.gene23321:CDS
CGACGAAGAAGGAGTGCGGT+TGG	0.523063	4.4:-37542593	MS.gene23321:CDS
GGTGCTCACTTTCTCTCGAC+CGG	0.523916	4.4:-37542103	MS.gene23321:CDS
CTTCTTCGTCGACCACCTGA+CGG	0.530090	4.4:+37542605	None:intergenic
TTCTTCGCGCATCACCCCAC+CGG	0.538068	4.4:+37541503	None:intergenic
CTTTAGCACTTACTAGCAAG+AGG	0.547021	4.4:+37541348	None:intergenic
ACCGTTCTTGACAAGATTAG+CGG	0.547122	4.4:+37541371	None:intergenic
AGTACAACCGGTGCACTTGA+AGG	0.552820	4.4:-37542124	MS.gene23321:CDS
AAACTCCCTCGGAGTAAGAT+CGG	0.553683	4.4:+37542408	None:intergenic
CATCAGACCACCATTGCACC+CGG	0.562811	4.4:+37541554	None:intergenic
CTATTCGCCTATTAGGATGA+AGG	0.564118	4.4:-37541122	MS.gene23321:CDS
TGCTCAAAGAGACTAGATCA+TGG	0.566764	4.4:-37541175	MS.gene23321:CDS
GTCTCTTTGAGCATATGTAT+GGG	0.587803	4.4:+37541185	None:intergenic
AACTCATGGGGTAAGACTTG+GGG	0.587990	4.4:-37541076	MS.gene23321:CDS
ATTTAAACATAAGTTTGGGA+AGG	0.587990	4.4:-37542548	MS.gene23321:CDS
GCCGCTAATCTTGTCAAGAA+CGG	0.589186	4.4:-37541372	MS.gene23321:CDS
GTGAGCACCTTCAAGTGCAC+CGG	0.592969	4.4:+37542117	None:intergenic
ACTTGTCGATTGTGATCATG+AGG	0.595475	4.4:-37542053	MS.gene23321:intron
TGGATACTACAAAATATGCA+GGG	0.599253	4.4:-37541047	MS.gene23321:CDS
GTATATATGCAAACATACAT+TGG	0.601664	4.4:-37541220	MS.gene23321:CDS
TGTGATGCCGGGTGCAATGG+TGG	0.601772	4.4:-37541561	MS.gene23321:CDS
TATATGCGGAGTGGACTCCA+TGG	0.603926	4.4:-37541017	MS.gene23321:CDS
ACGCTCTGACGAAACTCCCT+CGG	0.605558	4.4:+37542397	None:intergenic
ATTCTCCGATCTTACTCCGA+GGG	0.607597	4.4:-37542413	MS.gene23321:CDS
CACCCGGCATCACAAGAACC+CGG	0.609271	4.4:+37541570	None:intergenic
TATATGCAAACATACATTGG+AGG	0.612222	4.4:-37541217	MS.gene23321:CDS
CGGCGTAGAGAATGCCGAGA+CGG	0.623788	4.4:+37542651	None:intergenic
CTACTGAGTAGTAGTATGAA+CGG	0.624053	4.4:+37540979	None:intergenic
GGACTCCATGGTTTCTACTG+TGG	0.626354	4.4:-37541005	MS.gene23321:CDS
TCAATTGATGGATCCTTCGG+CGG	0.633662	4.4:-37542452	MS.gene23321:CDS
GATCATGGAGTGTTACTGGT+GGG	0.636411	4.4:-37541160	MS.gene23321:CDS
GTAACACCGTGAACCGCCGA+AGG	0.638844	4.4:+37542439	None:intergenic
TAGTGTGATCCAGAGCAACC+GGG	0.639888	4.4:-37541588	MS.gene23321:CDS
AGGGCTCGTGTGGATCATGT+TGG	0.640660	4.4:-37542153	MS.gene23321:CDS
GCATTCTCTACGCCGGACGA+AGG	0.643570	4.4:-37542643	MS.gene23321:CDS
ATCATGAATTAGGGCTCGTG+TGG	0.646039	4.4:-37542163	MS.gene23321:intron
GGCAGCCACAGTAGAAACCA+TGG	0.655273	4.4:+37541000	None:intergenic
CGTCTCGGCATTCTCTACGC+CGG	0.661935	4.4:-37542650	MS.gene23321:CDS
AGATCATGGAGTGTTACTGG+TGG	0.667806	4.4:-37541161	MS.gene23321:CDS
GGATACTACAAAATATGCAG+GGG	0.670950	4.4:-37541046	MS.gene23321:CDS
AGATCCAATAATCCGTCAGG+TGG	0.675027	4.4:-37542617	MS.gene23321:CDS
TGCAGGGGACGAAATATATG+CGG	0.679393	4.4:-37541031	MS.gene23321:CDS
GGGACGAAATATATGCGGAG+TGG	0.687467	4.4:-37541026	MS.gene23321:CDS
AATTCTCCGATCTTACTCCG+AGG	0.702571	4.4:-37542414	MS.gene23321:CDS
TGGTCGACGAAGAAGGAGTG+CGG	0.715673	4.4:-37542597	MS.gene23321:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATTATATGTTATCTAAAT+TGG	-	chr4.4:37542040-37542059	MS.gene23321:intron	10.0%
!!	AGATATACTATTAATTACAA+AGG	+	chr4.4:37541672-37541691	None:intergenic	15.0%
!!	ATCTTTACAGAATTATTTAT+TGG	-	chr4.4:37541974-37541993	MS.gene23321:intron	15.0%
!!	GATATACTATTAATTACAAA+GGG	+	chr4.4:37541671-37541690	None:intergenic	15.0%
!!	TCTTTACAGAATTATTTATT+GGG	-	chr4.4:37541975-37541994	MS.gene23321:intron	15.0%
!!	AACCTATTTAAACATAAGTT+TGG	-	chr4.4:37541123-37541142	MS.gene23321:CDS	20.0%
!!	AATTAATAGTATATCTGCTA+AGG	-	chr4.4:37541676-37541695	MS.gene23321:intron	20.0%
!!	ACCTATTTAAACATAAGTTT+GGG	-	chr4.4:37541124-37541143	MS.gene23321:CDS	20.0%
!!	ATACAATATCTAATAGAGTT+TGG	+	chr4.4:37541809-37541828	None:intergenic	20.0%
!!	ATATGTTATCTAAATTGGTT+TGG	-	chr4.4:37542045-37542064	MS.gene23321:intron	20.0%
!!	ATTAATTACAAAGGGAAAAT+AGG	+	chr4.4:37541663-37541682	None:intergenic	20.0%
!!	ATTGGATAATTAAAAACTCA+TGG	-	chr4.4:37542586-37542605	MS.gene23321:CDS	20.0%
!!	TATCTGATATTAATCTTTCT+AGG	-	chr4.4:37541753-37541772	MS.gene23321:intron	20.0%
!!	TTGGATAATTAAAAACTCAT+GGG	-	chr4.4:37542587-37542606	MS.gene23321:CDS	20.0%
!!!	AAATGCTAACTTGTAATATT+TGG	-	chr4.4:37541998-37542017	MS.gene23321:intron	20.0%
!!!	AATGCTAACTTGTAATATTT+GGG	-	chr4.4:37541999-37542018	MS.gene23321:intron	20.0%
!!!	GAGTTTTTAATTATCCAATA+TGG	+	chr4.4:37542585-37542604	None:intergenic	20.0%
!!!	TTTGAGTACATTTTAAAATC+CGG	-	chr4.4:37542154-37542173	MS.gene23321:CDS	20.0%
!	ACTCTATTAGATATTGTATG+AGG	-	chr4.4:37541810-37541829	MS.gene23321:intron	25.0%
!	ATTTAAACATAAGTTTGGGA+AGG	-	chr4.4:37541128-37541147	MS.gene23321:CDS	25.0%
!	GTATATATGCAAACATACAT+TGG	-	chr4.4:37542456-37542475	MS.gene23321:CDS	25.0%
!	TCCCAAACTTATGTTTAAAT+AGG	+	chr4.4:37541128-37541147	None:intergenic	25.0%
!	TGGATAATTAAAAACTCATG+GGG	-	chr4.4:37542588-37542607	MS.gene23321:CDS	25.0%
!	TTGCATATATACTGCATTTA+AGG	+	chr4.4:37542448-37542467	None:intergenic	25.0%
!!	AGCAAATTTGAAACATGATT+AGG	-	chr4.4:37541906-37541925	MS.gene23321:intron	25.0%
!!	ATGCTAACTTGTAATATTTG+GGG	-	chr4.4:37542000-37542019	MS.gene23321:intron	25.0%
!!	TGTTTTGTGATCATGAATTA+GGG	-	chr4.4:37541504-37541523	MS.gene23321:CDS	25.0%
!!!	TTATCTAAATTGGTTTGGAT+TGG	-	chr4.4:37542050-37542069	MS.gene23321:intron	25.0%
	AGTTACTGCTGTTAAAAATC+AGG	-	chr4.4:37541404-37541423	MS.gene23321:CDS	30.0%
	ATGGATACTACAAAATATGC+AGG	-	chr4.4:37542628-37542647	MS.gene23321:CDS	30.0%
	GAAGAAGATTATCCTTATTC+TGG	-	chr4.4:37542190-37542209	MS.gene23321:intron	30.0%
	TATATGCAAACATACATTGG+AGG	-	chr4.4:37542459-37542478	MS.gene23321:CDS	30.0%
	TGGATACTACAAAATATGCA+GGG	-	chr4.4:37542629-37542648	MS.gene23321:CDS	30.0%
!	GTGTTTTGTGATCATGAATT+AGG	-	chr4.4:37541503-37541522	MS.gene23321:CDS	30.0%
!!!	AGTACATTTTAAAATCCGGT+GGG	-	chr4.4:37542158-37542177	MS.gene23321:intron	30.0%
	AGATTATCTGTCGGAAGAAT+CGG	+	chr4.4:37541351-37541370	None:intergenic	35.0%
	AGTCTCTTTGAGCATATGTA+TGG	+	chr4.4:37542495-37542514	None:intergenic	35.0%
	ATTGTATGAGGTTCGACTTA+AGG	-	chr4.4:37541822-37541841	MS.gene23321:intron	35.0%
	CTACTGAGTAGTAGTATGAA+CGG	+	chr4.4:37542700-37542719	None:intergenic	35.0%
	GGATACTACAAAATATGCAG+GGG	-	chr4.4:37542630-37542649	MS.gene23321:CDS	35.0%
	GTCTCTTTGAGCATATGTAT+GGG	+	chr4.4:37542494-37542513	None:intergenic	35.0%
	TCAATTGATGCTTCTTAGCA+CGG	+	chr4.4:37541212-37541231	None:intergenic	35.0%
!!	TGCTAAGAAGCATCAATTGA+TGG	-	chr4.4:37541212-37541231	MS.gene23321:CDS	35.0%
!!!	GAGTACATTTTAAAATCCGG+TGG	-	chr4.4:37542157-37542176	MS.gene23321:intron	35.0%
!!!	GATTTTGATTGGAGAGAGAA+AGG	-	chr4.4:37541378-37541397	MS.gene23321:CDS	35.0%
!!!	GTACATTTTAAAATCCGGTG+GGG	-	chr4.4:37542159-37542178	MS.gene23321:intron	35.0%
	AAAACTCATGGGGTAAGACT+TGG	-	chr4.4:37542598-37542617	MS.gene23321:CDS	40.0%
	AAACTCATGGGGTAAGACTT+GGG	-	chr4.4:37542599-37542618	MS.gene23321:CDS	40.0%
	ACCGTTCTTGACAAGATTAG+CGG	+	chr4.4:37542308-37542327	None:intergenic	40.0%
	AGAAGATCCAATAATCCGTC+AGG	-	chr4.4:37541056-37541075	MS.gene23321:CDS	40.0%
	ATTATCTGTCGGAAGAATCG+GGG	+	chr4.4:37541349-37541368	None:intergenic	40.0%
	CCAAGGATGAACATGATTAC+AGG	-	chr4.4:37541160-37541179	MS.gene23321:CDS	40.0%
	CCTGTAATCATGTTCATCCT+TGG	+	chr4.4:37541163-37541182	None:intergenic	40.0%
	CTATTCGCCTATTAGGATGA+AGG	-	chr4.4:37542554-37542573	MS.gene23321:CDS	40.0%
	CTTCATCCTAATAGGCGAAT+AGG	+	chr4.4:37542556-37542575	None:intergenic	40.0%
	CTTTAGCACTTACTAGCAAG+AGG	+	chr4.4:37542331-37542350	None:intergenic	40.0%
	GATTATCTGTCGGAAGAATC+GGG	+	chr4.4:37541350-37541369	None:intergenic	40.0%
	TCTCTCCAATCAAAATCCTC+CGG	+	chr4.4:37541375-37541394	None:intergenic	40.0%
	TGCTCAAAGAGACTAGATCA+TGG	-	chr4.4:37542501-37542520	MS.gene23321:CDS	40.0%
	TGGGAAGGTTTATTCTTCCA+AGG	-	chr4.4:37541143-37541162	MS.gene23321:CDS	40.0%
	TGTTGGAGTTTCAGTACAAC+CGG	-	chr4.4:37541540-37541559	MS.gene23321:CDS	40.0%
!	ACTAGATCATGGAGTGTTAC+TGG	-	chr4.4:37542512-37542531	MS.gene23321:CDS	40.0%
!	ACTTGTCGATTGTGATCATG+AGG	-	chr4.4:37541623-37541642	MS.gene23321:intron	40.0%
!	GCATCAATTGATGGATCCTT+CGG	-	chr4.4:37541221-37541240	MS.gene23321:CDS	40.0%
!	GCTAAGACTGACAAGTTTTC+CGG	+	chr4.4:37541595-37541614	None:intergenic	40.0%
!	TCTTTTCATCTTCTCCGTCT+CGG	-	chr4.4:37541011-37541030	MS.gene23321:CDS	40.0%
!!	GCACATTGATAGTGACTTGT+AGG	-	chr4.4:37541858-37541877	MS.gene23321:intron	40.0%
	AAACTCCCTCGGAGTAAGAT+CGG	+	chr4.4:37541271-37541290	None:intergenic	45.0%
	AACTCATGGGGTAAGACTTG+GGG	-	chr4.4:37542600-37542619	MS.gene23321:CDS	45.0%
	AATTCTCCGATCTTACTCCG+AGG	-	chr4.4:37541262-37541281	MS.gene23321:intron	45.0%
	AGATCCAATAATCCGTCAGG+TGG	-	chr4.4:37541059-37541078	MS.gene23321:CDS	45.0%
	ATAGTGTGATCCAGAGCAAC+CGG	-	chr4.4:37542087-37542106	MS.gene23321:CDS	45.0%
	ATCATGAATTAGGGCTCGTG+TGG	-	chr4.4:37541513-37541532	MS.gene23321:CDS	45.0%
	ATTCTCCGATCTTACTCCGA+GGG	-	chr4.4:37541263-37541282	MS.gene23321:intron	45.0%
	ATTGGATCTTCTCCTTCGTC+CGG	+	chr4.4:37541048-37541067	None:intergenic	45.0%
	CCACGATCAGTACCAGAATA+AGG	+	chr4.4:37542205-37542224	None:intergenic	45.0%
	CTCTCCAATCAAAATCCTCC+GGG	+	chr4.4:37541374-37541393	None:intergenic	45.0%
	GCCGCTAATCTTGTCAAGAA+CGG	-	chr4.4:37542304-37542323	MS.gene23321:intron	45.0%
	GGATGAAGGAGAAGCCATAT+TGG	-	chr4.4:37542568-37542587	MS.gene23321:CDS	45.0%
	GGCTTCTCCTTCATCCTAAT+AGG	+	chr4.4:37542564-37542583	None:intergenic	45.0%
	GGTAAGACTTGGGGAGAAAA+TGG	-	chr4.4:37542609-37542628	MS.gene23321:CDS	45.0%
	TCTTCCGACAGATAATCTCC+CGG	-	chr4.4:37541353-37541372	MS.gene23321:intron	45.0%
	TGACAAGATTAGCGGCAATC+TGG	+	chr4.4:37542300-37542319	None:intergenic	45.0%
	TGCAGGGGACGAAATATATG+CGG	-	chr4.4:37542645-37542664	MS.gene23321:CDS	45.0%
	TTACTGGTGGGATATGGATC+TGG	-	chr4.4:37542528-37542547	MS.gene23321:CDS	45.0%
!	AGATCATGGAGTGTTACTGG+TGG	-	chr4.4:37542515-37542534	MS.gene23321:CDS	45.0%
!	GATCATGGAGTGTTACTGGT+GGG	-	chr4.4:37542516-37542535	MS.gene23321:CDS	45.0%
!	TCAATTGATGGATCCTTCGG+CGG	-	chr4.4:37541224-37541243	MS.gene23321:CDS	45.0%
!!	ATCTCCCGGAGGATTTTGAT+TGG	-	chr4.4:37541367-37541386	MS.gene23321:CDS	45.0%
!!	CCTTATTCTGGTACTGATCG+TGG	-	chr4.4:37542202-37542221	MS.gene23321:intron	45.0%
!!	GAGTTTCGTCAGAGCGTTTT+GGG	-	chr4.4:37541285-37541304	MS.gene23321:intron	45.0%
!!!	AGAGCGTTTTGGGATTGAGA+GGG	-	chr4.4:37541295-37541314	MS.gene23321:intron	45.0%
	AGTACAACCGGTGCACTTGA+AGG	-	chr4.4:37541552-37541571	MS.gene23321:CDS	50.0%
	CTGGAGCCTATTCGCCTATT+AGG	-	chr4.4:37542547-37542566	MS.gene23321:CDS	50.0%
	GGACTCCATGGTTTCTACTG+TGG	-	chr4.4:37542671-37542690	MS.gene23321:CDS	50.0%
	GGGACGAAATATATGCGGAG+TGG	-	chr4.4:37542650-37542669	MS.gene23321:CDS	50.0%
	GGGGCTGCATTTGCATCTTT+CGG	+	chr4.4:37541330-37541349	None:intergenic	50.0%
	TAGTGTGATCCAGAGCAACC+GGG	-	chr4.4:37542088-37542107	MS.gene23321:CDS	50.0%
	TCCTCCGGGAGATTATCTGT+CGG	+	chr4.4:37541360-37541379	None:intergenic	50.0%
!	GGAGTGTTACTGGTGGGATA+TGG	-	chr4.4:37542522-37542541	MS.gene23321:CDS	50.0%
!	TATATGCGGAGTGGACTCCA+TGG	-	chr4.4:37542659-37542678	MS.gene23321:CDS	50.0%
!!	GGAGTTTCGTCAGAGCGTTT+TGG	-	chr4.4:37541284-37541303	MS.gene23321:intron	50.0%
!!	TCGACCACCTGACGGATTAT+TGG	+	chr4.4:37541066-37541085	None:intergenic	50.0%
!!!	CAGAGCGTTTTGGGATTGAG+AGG	-	chr4.4:37541294-37541313	MS.gene23321:intron	50.0%
!!!	GAGCGTTTTGGGATTGAGAG+GGG	-	chr4.4:37541296-37541315	MS.gene23321:intron	50.0%
!!!	GTTTTGGGATTGAGAGGGGT+GGG	-	chr4.4:37541300-37541319	MS.gene23321:intron	50.0%
!!!	TTTTGGGATTGAGAGGGGTG+GGG	-	chr4.4:37541301-37541320	MS.gene23321:intron	50.0%
	AACCGGGTTCTTGTGATGCC+GGG	-	chr4.4:37542104-37542123	MS.gene23321:CDS	55.0%
	ACGCTCTGACGAAACTCCCT+CGG	+	chr4.4:37541282-37541301	None:intergenic	55.0%
	AGGGCTCGTGTGGATCATGT+TGG	-	chr4.4:37541523-37541542	MS.gene23321:CDS	55.0%
	ATGGATCCTTCGGCGGTTCA+CGG	-	chr4.4:37541231-37541250	MS.gene23321:CDS	55.0%
	CAACCGGGTTCTTGTGATGC+CGG	-	chr4.4:37542103-37542122	MS.gene23321:CDS	55.0%
	CATCAGACCACCATTGCACC+CGG	+	chr4.4:37542125-37542144	None:intergenic	55.0%
	CTTCTTCGTCGACCACCTGA+CGG	+	chr4.4:37541074-37541093	None:intergenic	55.0%
	GGCAGCCACAGTAGAAACCA+TGG	+	chr4.4:37542679-37542698	None:intergenic	55.0%
	GGTGCTCACTTTCTCTCGAC+CGG	-	chr4.4:37541573-37541592	MS.gene23321:CDS	55.0%
	GTGAGCACCTTCAAGTGCAC+CGG	+	chr4.4:37541562-37541581	None:intergenic	55.0%
	TCACAAGAACCCGGTTGCTC+TGG	+	chr4.4:37542100-37542119	None:intergenic	55.0%
	TCCGACAGATAATCTCCCGG+AGG	-	chr4.4:37541356-37541375	MS.gene23321:intron	55.0%
	TCTTGTGATGCCGGGTGCAA+TGG	-	chr4.4:37542112-37542131	MS.gene23321:CDS	55.0%
	TGGTCGACGAAGAAGGAGTG+CGG	-	chr4.4:37541079-37541098	MS.gene23321:CDS	55.0%
!	GACGAAGAAGGAGTGCGGTT+GGG	-	chr4.4:37541084-37541103	MS.gene23321:CDS	55.0%
!!!	CGTTTTGGGATTGAGAGGGG+TGG	-	chr4.4:37541299-37541318	MS.gene23321:intron	55.0%
	CACCCGGCATCACAAGAACC+CGG	+	chr4.4:37542109-37542128	None:intergenic	60.0%
	CGGCGTAGAGAATGCCGAGA+CGG	+	chr4.4:37541028-37541047	None:intergenic	60.0%
	CGTCAGGTGGTCGACGAAGA+AGG	-	chr4.4:37541072-37541091	MS.gene23321:CDS	60.0%
	CGTCTCGGCATTCTCTACGC+CGG	-	chr4.4:37541026-37541045	MS.gene23321:CDS	60.0%
	GCATTCTCTACGCCGGACGA+AGG	-	chr4.4:37541033-37541052	MS.gene23321:CDS	60.0%
	GTAACACCGTGAACCGCCGA+AGG	+	chr4.4:37541240-37541259	None:intergenic	60.0%
	TGTGATGCCGGGTGCAATGG+TGG	-	chr4.4:37542115-37542134	MS.gene23321:CDS	60.0%
	TTCTTCGCGCATCACCCCAC+CGG	+	chr4.4:37542176-37542195	None:intergenic	60.0%
!	CGACGAAGAAGGAGTGCGGT+TGG	-	chr4.4:37541083-37541102	MS.gene23321:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	37540979	37542719	37540979	ID=MS.gene23321
chr4.4	mRNA	37540979	37542719	37540979	ID=MS.gene23321.t1;Parent=MS.gene23321
chr4.4	exon	37542381	37542719	37542381	ID=MS.gene23321.t1.exon1;Parent=MS.gene23321.t1
chr4.4	CDS	37542381	37542719	37542381	ID=cds.MS.gene23321.t1;Parent=MS.gene23321.t1
chr4.4	exon	37542273	37542320	37542273	ID=MS.gene23321.t1.exon2;Parent=MS.gene23321.t1
chr4.4	CDS	37542273	37542320	37542273	ID=cds.MS.gene23321.t1;Parent=MS.gene23321.t1
chr4.4	exon	37542054	37542173	37542054	ID=MS.gene23321.t1.exon3;Parent=MS.gene23321.t1
chr4.4	CDS	37542054	37542173	37542054	ID=cds.MS.gene23321.t1;Parent=MS.gene23321.t1
chr4.4	exon	37541361	37541607	37541361	ID=MS.gene23321.t1.exon4;Parent=MS.gene23321.t1
chr4.4	CDS	37541361	37541607	37541361	ID=cds.MS.gene23321.t1;Parent=MS.gene23321.t1
chr4.4	exon	37540979	37541256	37540979	ID=MS.gene23321.t1.exon5;Parent=MS.gene23321.t1
chr4.4	CDS	37540979	37541256	37540979	ID=cds.MS.gene23321.t1;Parent=MS.gene23321.t1

Gene Sequence

>MS.gene23321

ATGGATCATCGCACTCTTCTTCTCTTCGTCGTTCTTTTCATCTTCTCCGTCTCGGCATTCTCTACGCCGGACGAAGGAGAAGATCCAATAATCCGTCAGGTGGTCGACGAAGAAGGAGTGCGGTTGGGAGCAGAGCATCACTTTAACCTATTTAAACATAAGTTTGGGAAGGTTTATTCTTCCAAGGATGAACATGATTACAGGTTTAAAATCTTCAAGTCTAATCTGAACCGTGCTAAGAAGCATCAATTGATGGATCCTTCGGCGGTTCACGGTGTTACGAAATTCTCCGATCTTACTCCGAGGGAGTTTCGTCAGAGCGTTTTGGGATTGAGAGGGGTGGGGTTGCCGAAAGATGCAAATGCAGCCCCGATTCTTCCGACAGATAATCTCCCGGAGGATTTTGATTGGAGAGAGAAAGGAGCAGTTACTGCTGTTAAAAATCAGGTATTATTTCTAATTGTGATTATGATTATTATTTGTTTATTATTGTTGTTTCGTTTAAAAAAAAACAAATTTTGATTGTGTTTTGTGATCATGAATTAGGGCTCGTGTGGATCATGTTGGAGTTTCAGTACAACCGGTGCACTTGAAGGTGCTCACTTTCTCTCGACCGGAAAACTTGTCAGTCTTAGCGAGCAACAACTTGTCGATTGTGATCATGAGGTTTTTTCTTCTTTTCCTATTTTCCCTTTGTAATTAATAGTATATCTGCTAAGGAATTATGATTATGATTATAATTGTGATTGTCATACTGCATTTTGTAAAGAACTTTATCTGATATTAATCTTTCTAGGAAAACAAGTAACAAAAATAACATTTAGTAACCAAACTCTATTAGATATTGTATGAGGTTCGACTTAAGGAATCTCTGCAGTTGCACATTGATAGTGACTTGTAGGAAAGAAGACAAAAACACGTACAATAAGCAAATTTGAAACATGATTAGGTCATATGAATTTGTTTTAGTAATTGAACATATTTCAAATTAATTAATCTTTACAGAATTATTTATTGGGAAATGCTAACTTGTAATATTTGGGGCTTTATAGCAAGTTATATTATTATATGTTATCTAAATTGGTTTGGATTGGATTTACTTTTATGTATAGTGTGATCCAGAGCAACCGGGTTCTTGTGATGCCGGGTGCAATGGTGGTCTGATGAATAGTGCTTTTGAGTACATTTTAAAATCCGGTGGGGTGATGCGCGAAGAAGATTATCCTTATTCTGGTACTGATCGTGGAGCATGCAAGTTTGACAAGAAAAAAATTGCAGCATCAATTGCAAATTTCAGCGTTGTTTCACTTGATGAAGACCAGATTGCCGCTAATCTTGTCAAGAACGGTCCTCTTGCTAGTAAGTGCTAAAGTCTTAACAATTTGTGTTTTTTTGTTCAATTATAATTATCGAATTCAAATGCAGATATTGATTCTTGTAAATTTCAATGTGCACAAACGCAGTTGCCTTAAATGCAGTATATATGCAAACATACATTGGAGGAGTATCATGCCCATACATATGCTCAAAGAGACTAGATCATGGAGTGTTACTGGTGGGATATGGATCTGGAGCCTATTCGCCTATTAGGATGAAGGAGAAGCCATATTGGATAATTAAAAACTCATGGGGTAAGACTTGGGGAGAAAATGGATACTACAAAATATGCAGGGGACGAAATATATGCGGAGTGGACTCCATGGTTTCTACTGTGGCTGCCGTTCATACTACTACTCAGTAG

Protein sequence

>MS.gene23321.t1

MDHRTLLLFVVLFIFSVSAFSTPDEGEDPIIRQVVDEEGVRLGAEHHFNLFKHKFGKVYSSKDEHDYRFKIFKSNLNRAKKHQLMDPSAVHGVTKFSDLTPREFRQSVLGLRGDFDWREKGAVTAVKNQGSCGSCWSFSTTGALEGAHFLSTGKLVSLSEQQLVDCDHECDPEQPGSCDAGCNGGLMNSAFEYILKSGGVMREEDYPYSGTDRGACKFDKKKIAASIANFSVVSLDEDQIAANLVKNGPLAIALNAVYMQTYIGGVSCPYICSKRLDHGVLLVGYGSGAYSPIRMKEKPYWIIKNSWGKTWGENGYYKICRGRNICGVDSMVSTVAAVHTTTQ