AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043473

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043473.t1 MTR_2g090890 93.195 338 23 0 1 338 1 338 0.0 672
MS.gene043473.t1 MTR_4g079440 61.652 339 127 2 1 336 6 344 4.63e-158 446
MS.gene043473.t1 MTR_5g022560 51.622 339 150 5 5 335 10 342 2.24e-119 348
MS.gene043473.t1 MTR_4g107930 51.952 333 153 4 10 335 11 343 1.21e-118 346
MS.gene043473.t1 MTR_3g075390 50.720 347 158 5 3 338 2 346 1.25e-118 347
MS.gene043473.t1 MTR_4g079770 51.411 319 149 3 23 335 25 343 1.05e-115 338
MS.gene043473.t1 MTR_4g047610 53.074 309 138 3 35 336 34 342 2.34e-115 338
MS.gene043473.t1 MTR_4g079470 53.443 305 136 3 37 335 39 343 1.53e-114 336
MS.gene043473.t1 MTR_4g094918 46.957 345 169 6 4 338 3 343 2.25e-114 336
MS.gene043473.t1 MTR_4g079800 50.625 320 152 3 23 336 25 344 8.10e-114 334
MS.gene043473.t1 MTR_4g080730 51.316 304 144 3 35 335 36 338 8.81e-114 333
MS.gene043473.t1 MTR_1g107550 51.603 312 141 4 35 337 46 356 2.43e-112 335
MS.gene043473.t1 MTR_4g080700 50.987 304 145 3 35 335 36 338 1.88e-111 328
MS.gene043473.t1 MTR_1g018840 48.624 327 156 5 22 338 39 363 9.68e-109 325
MS.gene043473.t1 MTR_4g104370 52.597 308 134 6 39 337 40 344 1.29e-108 321
MS.gene043473.t1 MTR_1g018840 48.624 327 156 5 22 338 39 363 1.32e-108 325
MS.gene043473.t1 MTR_3g102220 49.841 315 145 5 35 337 49 362 2.34e-106 319
MS.gene043473.t1 MTR_2g075830 50.654 306 145 4 35 335 35 339 4.33e-106 314
MS.gene043473.t1 MTR_4g080360 46.131 336 171 5 9 336 8 341 8.74e-105 311
MS.gene043473.t1 MTR_3g116080 50.658 304 143 3 39 337 47 348 2.13e-104 310
MS.gene043473.t1 MTR_2g083930 47.284 313 154 3 35 336 31 343 1.32e-102 305
MS.gene043473.t1 MTR_3g112430 48.387 310 150 3 37 337 34 342 4.73e-98 297
MS.gene043473.t1 MTR_7g078600 48.397 312 148 8 31 336 33 337 1.43e-95 287
MS.gene043473.t1 MTR_7g079230 43.750 336 181 5 2 335 5 334 6.76e-92 278
MS.gene043473.t1 MTR_2g016180 40.968 310 170 5 39 338 51 357 1.98e-79 251
MS.gene043473.t1 MTR_2g016270 41.234 308 161 6 37 338 49 342 1.14e-78 248
MS.gene043473.t1 MTR_2g015850 44.828 290 148 7 55 336 78 363 5.14e-77 241
MS.gene043473.t1 MTR_4g065077 38.997 359 185 13 1 337 11 357 3.56e-73 231
MS.gene043473.t1 MTR_2g015920 40.635 315 165 9 39 338 53 360 2.49e-72 229
MS.gene043473.t1 MTR_2g067900 43.396 318 155 11 38 336 15 326 1.41e-69 220
MS.gene043473.t1 MTR_4g125300 42.105 304 164 7 39 336 51 348 8.79e-69 219
MS.gene043473.t1 MTR_2g015930 38.855 332 180 10 22 338 37 360 4.95e-67 215
MS.gene043473.t1 MTR_8g086470 38.136 354 171 13 5 337 31 357 1.79e-66 213
MS.gene043473.t1 MTR_2g067560 41.509 318 152 12 38 330 15 323 2.64e-62 213
MS.gene043473.t1 MTR_2g064475 38.261 345 154 13 38 334 15 348 1.31e-61 201
MS.gene043473.t1 MTR_1g023210 37.584 298 164 7 39 317 51 345 3.17e-60 197
MS.gene043473.t1 MTR_1g094135 37.025 316 166 9 36 328 53 358 2.45e-59 196
MS.gene043473.t1 MTR_4g071060 34.493 345 187 8 4 317 5 341 3.54e-58 192
MS.gene043473.t1 MTR_2g066980 34.831 356 163 13 7 335 4 317 7.43e-56 185
MS.gene043473.t1 MTR_1g023810 44.749 219 113 6 119 334 48 261 1.16e-55 182
MS.gene043473.t1 MTR_7g078630 40.000 270 124 6 35 292 702 945 2.39e-52 186
MS.gene043473.t1 MTR_4g072790 31.532 333 178 10 2 317 6 305 2.18e-47 163
MS.gene043473.t1 MTR_4g073660 30.564 337 180 10 2 317 5 308 2.00e-45 158
MS.gene043473.t1 MTR_4g072340 33.447 293 152 9 38 317 47 309 4.83e-45 157
MS.gene043473.t1 MTR_1g025450 41.379 174 79 6 119 289 54 207 6.02e-35 134
MS.gene043473.t1 MTR_7g078580 52.475 101 46 2 239 338 4 103 1.36e-31 115
MS.gene043473.t1 MTR_7g079130 57.143 91 38 1 247 336 7 97 9.75e-31 112
MS.gene043473.t1 MTR_2g015840 49.550 111 54 2 226 335 161 270 4.50e-30 115
MS.gene043473.t1 MTR_2g015840 39.264 163 84 7 1 155 1 156 5.46e-27 107
MS.gene043473.t1 MTR_2g016380 38.211 123 72 2 219 338 16 137 1.49e-24 100
MS.gene043473.t1 MTR_7g111060 29.137 278 159 12 86 333 66 335 2.70e-24 102
MS.gene043473.t1 MTR_7g111050 30.742 283 154 14 86 335 63 336 3.53e-24 101
MS.gene043473.t1 MTR_7g078310 42.336 137 72 4 79 212 114 246 2.04e-23 97.8
MS.gene043473.t1 MTR_3g029560 29.245 212 121 6 131 336 49 237 3.58e-23 96.7
MS.gene043473.t1 MTR_8g087510 41.667 132 70 5 161 290 23 149 8.85e-20 85.5
MS.gene043473.t1 MTR_2g047960 50.633 79 38 1 130 208 8 85 3.28e-17 76.3
MS.gene043473.t1 MTR_7g111050 28.862 246 139 12 84 298 56 296 6.43e-15 74.7
MS.gene043473.t1 MTR_7g079260 47.222 72 21 2 263 333 7 62 3.22e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043473.t1 AT1G06260 52.367 338 156 3 3 337 8 343 5.33e-129 372
MS.gene043473.t1 AT3G48340 50.585 342 156 4 7 337 5 344 2.48e-116 341
MS.gene043473.t1 AT5G43060 51.402 321 148 4 24 337 35 354 5.76e-115 341
MS.gene043473.t1 AT5G45890 49.263 339 157 6 10 335 8 344 3.18e-114 335
MS.gene043473.t1 AT1G47128 48.711 349 162 6 6 338 7 354 4.54e-114 338
MS.gene043473.t1 AT5G50260 48.703 347 166 5 1 338 1 344 9.99e-113 332
MS.gene043473.t1 AT3G19390 48.387 341 168 6 4 337 7 346 2.98e-111 331
MS.gene043473.t1 AT4G35350 51.757 313 145 3 30 337 42 353 2.44e-108 320
MS.gene043473.t1 AT1G20850 51.475 305 141 2 39 337 51 354 1.36e-107 318
MS.gene043473.t1 AT3G19400 49.062 320 156 6 25 337 30 349 5.68e-106 315
MS.gene043473.t1 AT3G48350 47.851 349 165 7 1 337 1 344 1.84e-104 311
MS.gene043473.t1 AT4G36880 52.397 292 126 6 59 338 73 363 4.82e-104 310
MS.gene043473.t1 AT4G11310 47.403 308 152 3 39 338 49 354 2.93e-100 300
MS.gene043473.t1 AT4G11320 47.078 308 153 3 39 338 56 361 4.50e-99 297
MS.gene043473.t1 AT4G11320 47.078 308 153 3 39 338 56 361 4.50e-99 297
MS.gene043473.t1 AT2G27420 44.886 352 173 6 1 336 1 347 1.39e-98 295
MS.gene043473.t1 AT3G49340 45.614 342 169 7 8 336 3 340 4.77e-98 293
MS.gene043473.t1 AT1G09850 47.727 308 155 4 35 337 28 334 4.69e-97 294
MS.gene043473.t1 AT4G23520 41.040 346 193 6 1 338 9 351 4.72e-97 291
MS.gene043473.t1 AT2G34080 47.634 317 144 7 35 336 35 344 3.48e-94 284
MS.gene043473.t1 AT1G29080 43.895 344 174 9 5 336 9 345 5.08e-92 278
MS.gene043473.t1 AT1G29090 42.319 345 178 9 5 336 18 354 3.32e-86 264
MS.gene043473.t1 AT3G43960 42.857 322 172 8 26 337 28 347 1.26e-82 255
MS.gene043473.t1 AT4G35350 50.403 248 116 4 30 272 42 287 2.64e-77 239
MS.gene043473.t1 AT3G19400 49.798 247 114 7 27 266 35 278 9.78e-74 229
MS.gene043473.t1 AT5G60360 43.934 305 157 8 39 336 59 356 6.90e-73 230
MS.gene043473.t1 AT1G29110 38.372 344 181 8 5 336 9 333 2.37e-72 228
MS.gene043473.t1 AT3G45310 38.843 363 188 10 1 336 1 356 2.44e-72 228
MS.gene043473.t1 AT5G60360 43.279 305 158 8 39 336 59 355 1.33e-70 224
MS.gene043473.t1 AT3G45310 38.567 363 188 10 1 336 1 355 2.77e-70 223
MS.gene043473.t1 AT3G48350 44.681 282 140 6 1 271 1 277 1.74e-69 219
MS.gene043473.t1 AT5G60360 44.211 285 149 7 39 316 59 340 8.17e-69 219
MS.gene043473.t1 AT4G39090 36.513 304 171 6 32 315 42 343 6.66e-61 199
MS.gene043473.t1 AT2G21430 33.728 338 193 8 5 315 7 340 9.63e-60 196
MS.gene043473.t1 AT3G54940 36.928 306 163 8 36 317 48 347 5.39e-58 192
MS.gene043473.t1 AT4G16190 36.246 309 173 7 39 328 55 358 5.64e-52 176
MS.gene043473.t1 AT3G54940 37.391 230 131 4 36 253 48 276 1.97e-43 151
MS.gene043473.t1 AT1G02305 30.935 278 148 13 84 328 67 333 8.52e-26 106
MS.gene043473.t1 AT4G01610 28.623 276 157 12 84 328 64 330 3.70e-24 101
MS.gene043473.t1 AT4G01610 27.899 276 159 12 84 328 64 330 3.57e-22 96.3
MS.gene043473.t1 AT1G02300 30.516 213 117 9 139 328 146 350 1.47e-16 80.1
MS.gene043473.t1 AT2G22160 39.326 89 50 1 55 139 10 98 4.55e-13 65.1

Find 69 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTCTTTGTGTGCATTTC+GGG 0.181188 2.2:-8552776 None:intergenic
TAATGTTATAGTTGTCTTCA+TGG 0.274993 2.2:-8552711 None:intergenic
TGAATTCTTTGTGTGCATTT+CGG 0.283355 2.2:-8552777 None:intergenic
TACCTCTTCTTCATGATTGC+AGG 0.284385 2.2:-8552806 None:intergenic
GCATTGTTATACTCAATCTA+TGG 0.301011 2.2:+8552735 MS.gene043473:CDS
TTACCGTCTCTTCCTGTATA+AGG 0.353740 2.2:-8553718 None:intergenic
GTTGGGTATGGAGAAGAAAA+TGG 0.381977 2.2:+8553949 MS.gene043473:CDS
GGCAAATGACTGGGGTGAAT+CGG 0.383051 2.2:+8554002 MS.gene043473:CDS
GAGTTCAAAAGCACCTATCT+AGG 0.384448 2.2:+8552992 MS.gene043473:CDS
CATGCTGCTTAATGCTTGAC+AGG 0.390203 2.2:-8554096 None:intergenic
TATTCAAAAGGTGTCTTCTC+TGG 0.393618 2.2:+8553889 MS.gene043473:CDS
ATTGGATTGTGAAGAATTCA+TGG 0.401875 2.2:+8553980 MS.gene043473:CDS
CTATATACTGAACATTTGAT+TGG 0.414116 2.2:-8552901 None:intergenic
AACCATGGCATGACTATAGT+TGG 0.415037 2.2:+8553931 MS.gene043473:CDS
AGAATTCATGGGCAAATGAC+TGG 0.416096 2.2:+8553992 MS.gene043473:CDS
TCAGCGGTGGCAGCTGTAGA+AGG 0.416880 2.2:+8553535 MS.gene043473:CDS
AAGAAAATGGTGAAAAGTAT+TGG 0.423887 2.2:+8553962 MS.gene043473:CDS
GGCATCAACAAGATCAAAAC+AGG 0.429365 2.2:+8553556 MS.gene043473:CDS
GCAAATGACTGGGGTGAATC+GGG 0.433050 2.2:+8554003 MS.gene043473:CDS
CCGAGGCAGAAAACCTAGAT+AGG 0.436245 2.2:-8553005 None:intergenic
CTTACAAGCTCAAAGACAAC+AGG 0.445738 2.2:+8552948 MS.gene043473:CDS
GTCGCCATTGCAACTGATGC+TGG 0.448142 2.2:+8553847 MS.gene043473:CDS
AACTACCGAAAAGCATAGAT+TGG 0.448513 2.2:+8553068 MS.gene043473:CDS
GCTAAAGAATATCCTTATAC+AGG 0.450987 2.2:+8553706 MS.gene043473:CDS
TTACAGAGACAGAGAAGAAT+GGG 0.469400 2.2:+8552859 MS.gene043473:CDS
ACTTGATGCAGGAAGTTGTT+GGG 0.479460 2.2:+8553507 MS.gene043473:intron
GGGGTGAATCGGGTTATATC+AGG 0.482885 2.2:+8554013 MS.gene043473:CDS
AGCATAGATTGGAGAAAGAA+TGG 0.483674 2.2:+8553079 MS.gene043473:CDS
GCATCAGTTGCAATGGCGAC+AGG 0.488969 2.2:-8553844 None:intergenic
CAGGATGAAACGTGATACTA+AGG 0.505062 2.2:+8554032 MS.gene043473:CDS
AACTTGATGCAGGAAGTTGT+TGG 0.520464 2.2:+8553506 MS.gene043473:intron
GGCATGACTATAGTTGGGTA+TGG 0.523474 2.2:+8553937 MS.gene043473:CDS
ACCATGGCATGACTATAGTT+GGG 0.527315 2.2:+8553932 MS.gene043473:CDS
AACAATGCTGTAACTATAAG+CGG 0.529987 2.2:+8553763 MS.gene043473:CDS
GTTACACACGTCAAAGATCA+AGG 0.535305 2.2:+8553106 MS.gene043473:CDS
CTATAGTCATGCCATGGTTG+AGG 0.539074 2.2:-8553927 None:intergenic
ATTACAGAGACAGAGAAGAA+TGG 0.541853 2.2:+8552858 MS.gene043473:CDS
GAATTCATGGGCAAATGACT+GGG 0.555476 2.2:+8553993 MS.gene043473:CDS
ATAGATTGCGATACTAAAAG+TGG 0.565210 2.2:+8553610 MS.gene043473:CDS
GGGAACGAAGGCTGCAAAGG+TGG 0.565824 2.2:+8553631 MS.gene043473:CDS
ATCCTGCAATCATGAAGAAG+AGG 0.570589 2.2:+8552804 MS.gene043473:CDS
GAAACATGGCTGAAACGATA+CGG 0.571747 2.2:+8552830 MS.gene043473:CDS
ACCTCCAGCATCAGTTGCAA+TGG 0.572861 2.2:-8553851 None:intergenic
CGTGATACTAAGGATAAAGA+CGG 0.580833 2.2:+8554042 MS.gene043473:CDS
GGTGTCTTCTCTGGAAGTTG+TGG 0.581965 2.2:+8553898 MS.gene043473:CDS
AGTGGGAACGAAGGCTGCAA+AGG 0.593884 2.2:+8553628 MS.gene043473:CDS
TTGGATTGTGAAGAATTCAT+GGG 0.600522 2.2:+8553981 MS.gene043473:CDS
CTTCTCTGGAAGTTGTGGAA+AGG 0.605603 2.2:+8553903 MS.gene043473:CDS
GACAGGGTATGTAGCATCCA+CGG 0.614493 2.2:-8554079 None:intergenic
ATAAGGATATTCTTTAGCAG+TGG 0.618035 2.2:-8553701 None:intergenic
TATCCTTATACAGGAAGAGA+CGG 0.621774 2.2:+8553715 MS.gene043473:CDS
TTGTTGGGCATTCTCAGCGG+TGG 0.623201 2.2:+8553522 MS.gene043473:CDS
GTGATACTAAGGATAAAGAC+GGG 0.625784 2.2:+8554043 MS.gene043473:CDS
CGGGACTTGTGGCATTGCCG+TGG 0.626460 2.2:+8554062 MS.gene043473:CDS
TCTGTTTGTGCACGAAACCG+AGG 0.626529 2.2:-8553022 None:intergenic
AAGGATAAAGACGGGACTTG+TGG 0.629104 2.2:+8554051 MS.gene043473:CDS
TGAAGAAGAGGTATGAAACA+TGG 0.632545 2.2:+8552816 MS.gene043473:CDS
GAATTCAGTTATCATAAACA+TGG 0.645354 2.2:+8553043 MS.gene043473:CDS
ACCCAACTATAGTCATGCCA+TGG 0.645927 2.2:-8553933 None:intergenic
TAGATTGCGATACTAAAAGT+GGG 0.654963 2.2:+8553611 MS.gene043473:CDS
GTCAAAGATCAAGGCCATTG+TGG 0.656255 2.2:+8553115 MS.gene043473:CDS
TTTGCAATAAACTACCACAA+TGG 0.657523 2.2:-8553129 None:intergenic
AAGAGACGGTAAGTGTAACA+AGG 0.663083 2.2:+8553729 MS.gene043473:CDS
TGTGGAAAGGACCTCAACCA+TGG 0.667027 2.2:+8553916 MS.gene043473:CDS
ATGCTGCTTAATGCTTGACA+GGG 0.670927 2.2:-8554095 None:intergenic
GATACTAAAAGTGGGAACGA+AGG 0.681544 2.2:+8553619 MS.gene043473:CDS
AAGTTGTTGGGCATTCTCAG+CGG 0.690067 2.2:+8553519 MS.gene043473:CDS
AATTCATGGGCAAATGACTG+GGG 0.692239 2.2:+8553994 MS.gene043473:CDS
GCCATTGCAACTGATGCTGG+AGG 0.719085 2.2:+8553850 MS.gene043473:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTGAAATTTATGTTTT+GGG + chr2.2:8553466-8553485 MS.gene043473:intron 10.0%
!!! AAATGAATAAACTTTTTTTG+AGG - chr2.2:8553182-8553201 None:intergenic 15.0%
!!! CATTTTTGAAATTTATGTTT+TGG + chr2.2:8553465-8553484 MS.gene043473:intron 15.0%
!! TTCAAATACATTTACTTGTT+AGG - chr2.2:8553393-8553412 None:intergenic 20.0%
!!! GCATTTCAATTTTATTCAAA+AGG + chr2.2:8553877-8553896 MS.gene043473:CDS 20.0%
! CTATATACTGAACATTTGAT+TGG - chr2.2:8552904-8552923 None:intergenic 25.0%
! GAATTCAGTTATCATAAACA+TGG + chr2.2:8553043-8553062 MS.gene043473:CDS 25.0%
! TTCAACTACATCAAAAAACA+CGG + chr2.2:8553670-8553689 MS.gene043473:CDS 25.0%
!! AAGAAAATGGTGAAAAGTAT+TGG + chr2.2:8553962-8553981 MS.gene043473:CDS 25.0%
AACAATGCTGTAACTATAAG+CGG + chr2.2:8553763-8553782 MS.gene043473:CDS 30.0%
ATAAGGATATTCTTTAGCAG+TGG - chr2.2:8553704-8553723 None:intergenic 30.0%
ATAGATTGCGATACTAAAAG+TGG + chr2.2:8553610-8553629 MS.gene043473:CDS 30.0%
ATTGGATTGTGAAGAATTCA+TGG + chr2.2:8553980-8553999 MS.gene043473:CDS 30.0%
GCATTGTTATACTCAATCTA+TGG + chr2.2:8552735-8552754 MS.gene043473:CDS 30.0%
GCTAAAGAATATCCTTATAC+AGG + chr2.2:8553706-8553725 MS.gene043473:CDS 30.0%
TAGATTGCGATACTAAAAGT+GGG + chr2.2:8553611-8553630 MS.gene043473:CDS 30.0%
TGAATTCTTTGTGTGCATTT+CGG - chr2.2:8552780-8552799 None:intergenic 30.0%
TTGGATTGTGAAGAATTCAT+GGG + chr2.2:8553981-8554000 MS.gene043473:CDS 30.0%
TTTGCAATAAACTACCACAA+TGG - chr2.2:8553132-8553151 None:intergenic 30.0%
! TTTCTCCAATCTATGCTTTT+CGG - chr2.2:8553076-8553095 None:intergenic 30.0%
!! ATTTATGTTTTGGGCTGATA+TGG + chr2.2:8553475-8553494 MS.gene043473:intron 30.0%
!!! TACAGCATTGTTTTTAGCTT+TGG - chr2.2:8553755-8553774 None:intergenic 30.0%
AACTACATCAAAAAACACGG+AGG + chr2.2:8553673-8553692 MS.gene043473:CDS 35.0%
ACTACATCAAAAAACACGGA+GGG + chr2.2:8553674-8553693 MS.gene043473:CDS 35.0%
ACTTATCGTTTAGTTGTGCA+TGG - chr2.2:8553264-8553283 None:intergenic 35.0%
AGCATAGATTGGAGAAAGAA+TGG + chr2.2:8553079-8553098 MS.gene043473:CDS 35.0%
ATTACAGAGACAGAGAAGAA+TGG + chr2.2:8552858-8552877 MS.gene043473:CDS 35.0%
CGTGATACTAAGGATAAAGA+CGG + chr2.2:8554042-8554061 MS.gene043473:CDS 35.0%
GAATTCTTTGTGTGCATTTC+GGG - chr2.2:8552779-8552798 None:intergenic 35.0%
GTGATACTAAGGATAAAGAC+GGG + chr2.2:8554043-8554062 MS.gene043473:CDS 35.0%
GTTTGTGATATGAACATGAC+AGG + chr2.2:8553306-8553325 MS.gene043473:intron 35.0%
TATCCTTATACAGGAAGAGA+CGG + chr2.2:8553715-8553734 MS.gene043473:CDS 35.0%
TGTTAGGTAGTAATTCCTCA+AGG - chr2.2:8553377-8553396 None:intergenic 35.0%
TTACAGAGACAGAGAAGAAT+GGG + chr2.2:8552859-8552878 MS.gene043473:CDS 35.0%
! AACTACCGAAAAGCATAGAT+TGG + chr2.2:8553068-8553087 MS.gene043473:CDS 35.0%
! GAAAACAGTCTTTTACCTTG+AGG + chr2.2:8553359-8553378 MS.gene043473:intron 35.0%
! TGAAGAAGAGGTATGAAACA+TGG + chr2.2:8552816-8552835 MS.gene043473:CDS 35.0%
!! TATTCAAAAGGTGTCTTCTC+TGG + chr2.2:8553889-8553908 MS.gene043473:CDS 35.0%
AACCATGGCATGACTATAGT+TGG + chr2.2:8553931-8553950 MS.gene043473:CDS 40.0%
AACTTGATGCAGGAAGTTGT+TGG + chr2.2:8553506-8553525 MS.gene043473:intron 40.0%
AAGAGACGGTAAGTGTAACA+AGG + chr2.2:8553729-8553748 MS.gene043473:CDS 40.0%
AATTCATGGGCAAATGACTG+GGG + chr2.2:8553994-8554013 MS.gene043473:CDS 40.0%
ACCATGGCATGACTATAGTT+GGG + chr2.2:8553932-8553951 MS.gene043473:CDS 40.0%
ACTTGATGCAGGAAGTTGTT+GGG + chr2.2:8553507-8553526 MS.gene043473:intron 40.0%
AGAATTCATGGGCAAATGAC+TGG + chr2.2:8553992-8554011 MS.gene043473:CDS 40.0%
ATCCTGCAATCATGAAGAAG+AGG + chr2.2:8552804-8552823 MS.gene043473:CDS 40.0%
CAGGATGAAACGTGATACTA+AGG + chr2.2:8554032-8554051 MS.gene043473:CDS 40.0%
CTTACAAGCTCAAAGACAAC+AGG + chr2.2:8552948-8552967 MS.gene043473:CDS 40.0%
GAAACATGGCTGAAACGATA+CGG + chr2.2:8552830-8552849 MS.gene043473:CDS 40.0%
GAATTCATGGGCAAATGACT+GGG + chr2.2:8553993-8554012 MS.gene043473:CDS 40.0%
GATACTAAAAGTGGGAACGA+AGG + chr2.2:8553619-8553638 MS.gene043473:CDS 40.0%
GGCATCAACAAGATCAAAAC+AGG + chr2.2:8553556-8553575 MS.gene043473:CDS 40.0%
GTTACACACGTCAAAGATCA+AGG + chr2.2:8553106-8553125 MS.gene043473:CDS 40.0%
GTTGGGTATGGAGAAGAAAA+TGG + chr2.2:8553949-8553968 MS.gene043473:CDS 40.0%
TACCTCTTCTTCATGATTGC+AGG - chr2.2:8552809-8552828 None:intergenic 40.0%
TTACCGTCTCTTCCTGTATA+AGG - chr2.2:8553721-8553740 None:intergenic 40.0%
!! GAGTTCAAAAGCACCTATCT+AGG + chr2.2:8552992-8553011 MS.gene043473:CDS 40.0%
AAGGATAAAGACGGGACTTG+TGG + chr2.2:8554051-8554070 MS.gene043473:CDS 45.0%
AAGTTGTTGGGCATTCTCAG+CGG + chr2.2:8553519-8553538 MS.gene043473:CDS 45.0%
ACCCAACTATAGTCATGCCA+TGG - chr2.2:8553936-8553955 None:intergenic 45.0%
CTATAGTCATGCCATGGTTG+AGG - chr2.2:8553930-8553949 None:intergenic 45.0%
GGCATGACTATAGTTGGGTA+TGG + chr2.2:8553937-8553956 MS.gene043473:CDS 45.0%
GGTGATGTCAAACTTGATGC+AGG + chr2.2:8553496-8553515 MS.gene043473:intron 45.0%
GTCAAAGATCAAGGCCATTG+TGG + chr2.2:8553115-8553134 MS.gene043473:CDS 45.0%
! AGCATTTTCTCATTGCGAGC+AGG - chr2.2:8553802-8553821 None:intergenic 45.0%
! CCTATCTAGGTTTTCTGCCT+CGG + chr2.2:8553005-8553024 MS.gene043473:CDS 45.0%
! CTTCTCTGGAAGTTGTGGAA+AGG + chr2.2:8553903-8553922 MS.gene043473:CDS 45.0%
ACCTCCAGCATCAGTTGCAA+TGG - chr2.2:8553854-8553873 None:intergenic 50.0%
CCGAGGCAGAAAACCTAGAT+AGG - chr2.2:8553008-8553027 None:intergenic 50.0%
GACAGGGTATGTAGCATCCA+CGG - chr2.2:8554082-8554101 None:intergenic 50.0%
GCAAATGACTGGGGTGAATC+GGG + chr2.2:8554003-8554022 MS.gene043473:CDS 50.0%
GGCAAATGACTGGGGTGAAT+CGG + chr2.2:8554002-8554021 MS.gene043473:CDS 50.0%
GGGGTGAATCGGGTTATATC+AGG + chr2.2:8554013-8554032 MS.gene043473:CDS 50.0%
TCTGTTTGTGCACGAAACCG+AGG - chr2.2:8553025-8553044 None:intergenic 50.0%
TGTGGAAAGGACCTCAACCA+TGG + chr2.2:8553916-8553935 MS.gene043473:CDS 50.0%
! GGTGTCTTCTCTGGAAGTTG+TGG + chr2.2:8553898-8553917 MS.gene043473:CDS 50.0%
AGTGGGAACGAAGGCTGCAA+AGG + chr2.2:8553628-8553647 MS.gene043473:CDS 55.0%
GCATCAGTTGCAATGGCGAC+AGG - chr2.2:8553847-8553866 None:intergenic 55.0%
GCCATTGCAACTGATGCTGG+AGG + chr2.2:8553850-8553869 MS.gene043473:CDS 55.0%
GTCGCCATTGCAACTGATGC+TGG + chr2.2:8553847-8553866 MS.gene043473:CDS 55.0%
TTGTTGGGCATTCTCAGCGG+TGG + chr2.2:8553522-8553541 MS.gene043473:CDS 55.0%
GGGAACGAAGGCTGCAAAGG+TGG + chr2.2:8553631-8553650 MS.gene043473:CDS 60.0%
TCAGCGGTGGCAGCTGTAGA+AGG + chr2.2:8553535-8553554 MS.gene043473:CDS 60.0%
CGGGACTTGTGGCATTGCCG+TGG + chr2.2:8554062-8554081 MS.gene043473:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 8552713 8554110 8552713 ID=MS.gene043473
chr2.2 mRNA 8552713 8554110 8552713 ID=MS.gene043473.t1;Parent=MS.gene043473
chr2.2 exon 8552713 8553136 8552713 ID=MS.gene043473.t1.exon1;Parent=MS.gene043473.t1
chr2.2 CDS 8552713 8553136 8552713 ID=cds.MS.gene043473.t1;Parent=MS.gene043473.t1
chr2.2 exon 8553518 8554110 8553518 ID=MS.gene043473.t1.exon2;Parent=MS.gene043473.t1
chr2.2 CDS 8553518 8554110 8553518 ID=cds.MS.gene043473.t1;Parent=MS.gene043473.t1
Gene Sequence

>MS.gene043473

ATGAAGACAACTATAACATTAAGCATTGTTATACTCAATCTATGGATAACTGCAAGTGCATGTCCCGAAATGCACACAAAGAATTCAACTAATCCTGCAATCATGAAGAAGAGGTATGAAACATGGCTGAAACGATACGGACGACATTACAGAGACAGAGAAGAATGGGAAGTTCGTTTCGACATCTACCAATCAAATGTTCAGTATATAGAGTTCTACAACTCTCAAAACTATTCTTACAAGCTCAAAGACAACAGGTTTGCAGATCTTACTAATGAAGAGTTCAAAAGCACCTATCTAGGTTTTCTGCCTCGGTTTCGTGCACAAACAGAATTCAGTTATCATAAACATGGAGAACTACCGAAAAGCATAGATTGGAGAAAGAATGGTGCAGTTACACACGTCAAAGATCAAGGCCATTGTGGTAGTTTATTGCAAATCAAGTTTATTAATTTTTTTTTTTTTGCCTCAAAAAAAGTTTATTCATTTTCTCAGATATTACAGTTTAGCTACTAGTATGCTATGATATATACACAAATCTAGTAGTTCCATGCACAACTAAACGATAAGTCTATACGAACTTTATCTGAAGTGTTTGTGATATGAACATGACAGGATAAATTAGTCTTATAGTTATATCTATAAAGAAAACAGTCTTTTACCTTGAGGAATTACTACCTAACAAGTAAATGTATTTGAAGTATTCTGAAGTTTATCTATTGCTAAGTAGCGAGCTAAAGAGCATGCTTAAACATTTTTGAAATTTATGTTTTGGGCTGATATGGTGATGTCAAACTTGATGCAGGAAGTTGTTGGGCATTCTCAGCGGTGGCAGCTGTAGAAGGCATCAACAAGATCAAAACAGGAAATTTAGTTTCTCTATCAGAACAACAGTTGATAGATTGCGATACTAAAAGTGGGAACGAAGGCTGCAAAGGTGGCGACATGTACATAGCATTCAACTACATCAAAAAACACGGAGGGTTAGCCACTGCTAAAGAATATCCTTATACAGGAAGAGACGGTAAGTGTAACAAGGCCAAAGCTAAAAACAATGCTGTAACTATAAGCGGCTACGAAAGTGTACCTGCTCGCAATGAGAAAATGCTAAAAGCTGCAGTTGCTCATCAGCCTGTCGCCATTGCAACTGATGCTGGAGGTTATGCATTTCAATTTTATTCAAAAGGTGTCTTCTCTGGAAGTTGTGGAAAGGACCTCAACCATGGCATGACTATAGTTGGGTATGGAGAAGAAAATGGTGAAAAGTATTGGATTGTGAAGAATTCATGGGCAAATGACTGGGGTGAATCGGGTTATATCAGGATGAAACGTGATACTAAGGATAAAGACGGGACTTGTGGCATTGCCGTGGATGCTACATACCCTGTCAAGCATTAA
Protein sequence

>MS.gene043473.t1

MKTTITLSIVILNLWITASACPEMHTKNSTNPAIMKKRYETWLKRYGRHYRDREEWEVRFDIYQSNVQYIEFYNSQNYSYKLKDNRFADLTNEEFKSTYLGFLPRFRAQTEFSYHKHGELPKSIDWRKNGAVTHVKDQGHCGSCWAFSAVAAVEGINKIKTGNLVSLSEQQLIDCDTKSGNEGCKGGDMYIAFNYIKKHGGLATAKEYPYTGRDGKCNKAKAKNNAVTISGYESVPARNEKMLKAAVAHQPVAIATDAGGYAFQFYSKGVFSGSCGKDLNHGMTIVGYGEENGEKYWIVKNSWANDWGESGYIRMKRDTKDKDGTCGIAVDATYPVKH