AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006682

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006682.t1 MTR_4g079770 90.725 345 30 2 1 343 1 345 0.0 660
MS.gene006682.t1 MTR_5g022560 88.081 344 40 1 1 343 1 344 0.0 647
MS.gene006682.t1 MTR_4g079470 91.049 324 28 1 21 343 22 345 0.0 626
MS.gene006682.t1 MTR_4g079800 90.432 324 30 1 21 343 22 345 0.0 623
MS.gene006682.t1 MTR_4g107930 85.797 345 47 2 1 343 1 345 0.0 621
MS.gene006682.t1 MTR_4g047610 87.791 344 40 2 1 343 1 343 0.0 617
MS.gene006682.t1 MTR_2g075830 76.453 344 77 3 1 343 1 341 0.0 538
MS.gene006682.t1 MTR_4g080730 61.207 348 122 7 1 343 1 340 2.02e-150 427
MS.gene006682.t1 MTR_4g080700 64.198 324 103 7 25 343 25 340 2.02e-150 427
MS.gene006682.t1 MTR_4g104370 57.143 343 135 10 6 341 5 342 1.82e-131 379
MS.gene006682.t1 MTR_2g083930 55.193 337 137 5 12 341 13 342 4.42e-131 378
MS.gene006682.t1 MTR_4g080360 53.687 339 151 5 8 343 7 342 2.12e-128 371
MS.gene006682.t1 MTR_3g075390 52.632 342 145 8 14 342 7 344 1.09e-126 367
MS.gene006682.t1 MTR_4g079440 55.806 310 130 4 33 341 40 343 1.59e-123 358
MS.gene006682.t1 MTR_3g116080 51.026 341 151 7 11 342 14 347 7.96e-120 350
MS.gene006682.t1 MTR_1g107550 55.769 312 130 5 35 341 46 354 1.21e-119 353
MS.gene006682.t1 MTR_4g094918 50.733 341 155 7 10 342 6 341 1.18e-118 347
MS.gene006682.t1 MTR_2g090890 50.442 339 155 5 10 342 5 336 2.19e-117 343
MS.gene006682.t1 MTR_7g078600 55.696 316 125 8 31 341 31 336 1.60e-116 341
MS.gene006682.t1 MTR_7g079230 51.818 330 147 7 16 341 13 334 3.52e-115 337
MS.gene006682.t1 MTR_3g102220 53.333 315 128 8 39 341 53 360 1.58e-112 335
MS.gene006682.t1 MTR_1g018840 52.716 313 131 6 39 341 55 360 2.75e-112 334
MS.gene006682.t1 MTR_1g018840 52.716 313 131 6 39 341 55 360 4.25e-112 334
MS.gene006682.t1 MTR_3g112430 50.804 311 139 5 40 342 37 341 8.56e-102 307
MS.gene006682.t1 MTR_2g015850 53.767 292 127 6 53 341 76 362 3.98e-100 300
MS.gene006682.t1 MTR_2g016180 45.665 346 166 11 11 342 18 355 5.17e-90 278
MS.gene006682.t1 MTR_2g016270 45.614 342 157 11 11 342 18 340 1.74e-88 274
MS.gene006682.t1 MTR_2g067900 44.479 317 154 11 38 340 15 323 3.60e-75 235
MS.gene006682.t1 MTR_2g067560 44.237 321 147 13 38 337 15 324 1.72e-71 239
MS.gene006682.t1 MTR_2g064475 40.571 350 150 16 38 342 15 351 2.48e-71 226
MS.gene006682.t1 MTR_2g015920 38.941 321 176 11 33 342 47 358 5.45e-70 223
MS.gene006682.t1 MTR_4g125300 41.118 304 163 8 45 343 57 349 1.33e-69 221
MS.gene006682.t1 MTR_4g065077 38.509 322 178 9 32 342 44 356 2.90e-65 211
MS.gene006682.t1 MTR_2g066980 35.754 358 168 13 7 342 1 318 3.00e-65 209
MS.gene006682.t1 MTR_7g078630 44.755 286 117 6 28 302 695 950 5.86e-65 221
MS.gene006682.t1 MTR_4g071060 37.017 362 186 13 9 339 3 353 8.67e-65 209
MS.gene006682.t1 MTR_1g023810 44.156 231 120 6 113 340 37 261 5.50e-63 201
MS.gene006682.t1 MTR_1g023210 37.278 338 168 11 23 334 33 352 1.24e-62 204
MS.gene006682.t1 MTR_2g015930 39.564 321 167 12 37 342 50 358 1.46e-62 203
MS.gene006682.t1 MTR_8g086470 37.500 320 168 9 32 342 60 356 6.36e-60 197
MS.gene006682.t1 MTR_1g094135 34.540 359 195 12 7 339 19 363 2.39e-54 182
MS.gene006682.t1 MTR_4g072790 34.459 296 157 9 47 339 56 317 2.21e-42 150
MS.gene006682.t1 MTR_7g078580 64.286 98 34 1 244 341 4 100 1.23e-41 142
MS.gene006682.t1 MTR_4g072340 30.337 356 196 11 1 339 1 321 2.20e-41 147
MS.gene006682.t1 MTR_4g073660 33.667 300 158 9 47 339 55 320 3.18e-41 147
MS.gene006682.t1 MTR_7g079130 70.000 90 27 0 252 341 7 96 1.12e-40 138
MS.gene006682.t1 MTR_2g015840 47.134 157 79 3 7 160 1 156 2.45e-40 143
MS.gene006682.t1 MTR_2g015840 40.201 199 99 6 162 342 75 271 9.28e-37 134
MS.gene006682.t1 MTR_1g025450 37.433 187 92 7 113 295 43 208 8.52e-33 128
MS.gene006682.t1 MTR_7g078310 43.662 142 70 3 80 218 113 247 1.53e-27 108
MS.gene006682.t1 MTR_2g016380 46.281 121 58 4 226 342 18 135 1.76e-26 105
MS.gene006682.t1 MTR_7g111060 26.032 315 186 12 55 334 28 330 2.83e-24 102
MS.gene006682.t1 MTR_3g029560 30.159 252 139 9 98 342 16 237 1.18e-22 95.1
MS.gene006682.t1 MTR_7g079260 59.722 72 13 1 268 339 7 62 2.47e-22 90.5
MS.gene006682.t1 MTR_7g111050 28.936 235 137 8 131 342 110 337 3.63e-22 96.3
MS.gene006682.t1 MTR_8g087510 41.221 131 70 5 166 294 23 148 6.61e-20 85.9
MS.gene006682.t1 MTR_2g047960 47.059 85 44 1 134 218 7 90 1.08e-17 77.8
MS.gene006682.t1 MTR_3g082370 26.442 208 133 5 129 321 4 206 1.53e-16 78.2
MS.gene006682.t1 MTR_7g111050 26.667 195 119 6 131 304 105 296 7.47e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006682.t1 AT5G45890 54.783 345 145 6 7 342 3 345 1.71e-135 389
MS.gene006682.t1 AT5G50260 53.913 345 134 7 14 341 5 341 1.44e-130 377
MS.gene006682.t1 AT3G48340 54.467 347 133 9 12 341 4 342 2.73e-125 364
MS.gene006682.t1 AT2G34080 50.877 342 156 6 10 343 8 345 2.68e-122 355
MS.gene006682.t1 AT4G35350 51.156 346 152 8 7 342 14 352 1.15e-118 347
MS.gene006682.t1 AT3G19390 51.205 332 157 5 13 342 17 345 1.88e-117 347
MS.gene006682.t1 AT5G43060 53.681 326 136 7 24 341 34 352 2.90e-116 345
MS.gene006682.t1 AT3G49340 49.711 346 148 8 16 343 4 341 1.28e-115 338
MS.gene006682.t1 AT2G27420 49.425 348 158 6 10 343 5 348 1.96e-114 336
MS.gene006682.t1 AT3G48350 53.943 317 136 7 32 342 31 343 6.03e-113 333
MS.gene006682.t1 AT1G47128 52.751 309 133 7 39 341 50 351 1.24e-112 335
MS.gene006682.t1 AT3G19400 50.814 307 146 5 39 342 44 348 8.59e-112 330
MS.gene006682.t1 AT1G29090 47.025 353 170 7 4 343 7 355 1.11e-111 329
MS.gene006682.t1 AT4G36880 50.462 325 142 6 32 342 42 361 1.76e-111 329
MS.gene006682.t1 AT1G06260 50.146 343 151 7 9 341 9 341 3.41e-111 327
MS.gene006682.t1 AT1G20850 50.633 316 143 6 33 342 45 353 6.26e-110 325
MS.gene006682.t1 AT1G29080 47.605 334 152 7 24 343 22 346 5.52e-107 317
MS.gene006682.t1 AT1G09850 49.677 310 152 4 33 341 26 332 8.42e-102 306
MS.gene006682.t1 AT4G23520 48.220 309 145 9 40 341 48 348 1.63e-100 301
MS.gene006682.t1 AT4G11310 46.945 311 147 7 40 341 50 351 3.82e-99 297
MS.gene006682.t1 AT4G11320 45.714 315 145 7 40 341 57 358 3.33e-98 295
MS.gene006682.t1 AT4G11320 45.714 315 145 7 40 341 57 358 3.33e-98 295
MS.gene006682.t1 AT1G29110 43.293 328 150 7 29 342 28 333 7.00e-89 270
MS.gene006682.t1 AT3G43960 43.675 332 177 8 16 341 18 345 1.94e-87 268
MS.gene006682.t1 AT4G35350 48.718 273 124 7 13 276 21 286 5.09e-84 256
MS.gene006682.t1 AT3G19400 48.963 241 119 4 39 276 44 283 9.84e-78 240
MS.gene006682.t1 AT5G60360 42.208 308 162 9 41 343 61 357 1.01e-74 234
MS.gene006682.t1 AT3G48350 50.598 251 114 7 32 276 31 277 1.33e-73 230
MS.gene006682.t1 AT5G60360 41.883 308 162 10 41 343 61 356 3.41e-72 228
MS.gene006682.t1 AT3G45310 41.158 311 161 11 41 343 61 357 2.01e-71 226
MS.gene006682.t1 AT5G60360 41.414 297 162 8 41 332 61 350 3.27e-70 223
MS.gene006682.t1 AT3G45310 40.836 311 161 12 41 343 61 356 7.28e-69 219
MS.gene006682.t1 AT2G21430 38.284 303 163 8 47 334 56 349 2.68e-62 203
MS.gene006682.t1 AT4G39090 38.462 312 160 11 47 339 59 357 1.60e-60 198
MS.gene006682.t1 AT3G54940 38.125 320 160 10 38 334 50 354 7.01e-59 194
MS.gene006682.t1 AT4G16190 37.621 311 164 9 48 339 64 363 6.78e-57 189
MS.gene006682.t1 AT3G54940 38.197 233 126 5 38 258 50 276 7.83e-43 150
MS.gene006682.t1 AT1G02305 28.146 302 166 12 68 334 48 333 6.99e-26 106
MS.gene006682.t1 AT4G01610 27.336 289 171 11 75 334 52 330 1.01e-22 97.8
MS.gene006682.t1 AT4G01610 26.990 289 172 11 75 334 52 330 1.80e-21 94.4
MS.gene006682.t1 AT1G02300 28.638 213 122 6 144 334 146 350 2.27e-20 91.3
MS.gene006682.t1 AT2G22160 39.785 93 53 2 53 144 8 98 5.06e-15 70.5

Find 84 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCCTGCTGCTACCATTAC+TGG 0.241381 4.1:-26445381 MS.gene006682:CDS
AATATGATATAATTGATTAT+TGG 0.245014 4.1:+26446159 None:intergenic
TAAGTTAAGTACTGGAAAAT+TGG 0.257813 4.1:-26445580 MS.gene006682:CDS
GCATCAATGGCTACAGAAAT+TGG 0.307524 4.1:+26445300 None:intergenic
ACAATGATGGAACCAAATAT+TGG 0.314081 4.1:-26445179 MS.gene006682:CDS
TCACCATGGCAAAGTGTATA+AGG 0.340501 4.1:-26446035 MS.gene006682:CDS
TGTTCCGTCCACACCTTGAT+AGG 0.347233 4.1:+26445425 None:intergenic
AAATTCATCATTCAAAATCA+TGG 0.364764 4.1:-26445471 MS.gene006682:CDS
TTGCAGTGCGTTCTCATTGT+TGG 0.372109 4.1:+26445341 None:intergenic
AGGTTGTGAGGGTGGTCTTA+TGG 0.376813 4.1:-26445505 MS.gene006682:CDS
GGTGTTACTGCTGTGGGATA+TGG 0.378598 4.1:-26445207 MS.gene006682:CDS
TTTGTTATTGTCAGCATTGT+TGG 0.400731 4.1:+26445949 None:intergenic
TCAATTATATCATATTTCAT+TGG 0.405472 4.1:-26446152 MS.gene006682:CDS
GGAACATGCAATGCTAATAA+AGG 0.418975 4.1:-26445408 MS.gene006682:CDS
ACTTTGTGGCATTGCAATGC+AGG 0.423561 4.1:-26445076 MS.gene006682:CDS
TGCCTTTATAGGATGTTGTT+GGG 0.430973 4.1:-26445637 MS.gene006682:intron
CACACCTTGATAGGGGTATT+CGG 0.435671 4.1:+26445434 None:intergenic
ATGCCTTTATAGGATGTTGT+TGG 0.435997 4.1:-26445638 MS.gene006682:intron
AAAACTCATGGGGAACCGAC+TGG 0.445030 4.1:-26445149 MS.gene006682:CDS
TACAAAAGTGGTGTCTTCAC+TGG 0.452446 4.1:-26445255 MS.gene006682:CDS
AGTGCGTTCTCATTGTTGGC+AGG 0.452809 4.1:+26445345 None:intergenic
GGGATTCATAAGTTAAGTAC+TGG 0.452855 4.1:-26445588 MS.gene006682:CDS
ACAATCAACAAGTTCTTGTT+CGG 0.454613 4.1:+26445548 None:intergenic
GGATGATATGAACTCCTCAT+TGG 0.466065 4.1:+26445889 None:intergenic
GTCGGATCCACTAGCATCAA+TGG 0.467753 4.1:+26445287 None:intergenic
TGCTCGTGTTTCTCATACAT+AGG 0.475196 4.1:+26446067 None:intergenic
TCAGCTATTGCGGCAACTGA+AGG 0.488495 4.1:-26445609 MS.gene006682:CDS
TTCCTCCAATCCATTGTGGA+TGG 0.490858 4.1:+26445791 None:intergenic
TGCTACCATTACTGGGTATG+AGG 0.491803 4.1:-26445373 MS.gene006682:CDS
CTCCTGCTGCTACCATTACT+GGG 0.496600 4.1:-26445380 MS.gene006682:CDS
ATATTTAAAAGTAGTTGTCC+TGG 0.500162 4.1:+26445829 None:intergenic
AAACTCATGGGGAACCGACT+GGG 0.502307 4.1:-26445148 MS.gene006682:CDS
ATGGGGAACCGACTGGGGTG+AGG 0.510047 4.1:-26445142 MS.gene006682:CDS
TTTCTTCCTCCAATCCATTG+TGG 0.510059 4.1:+26445787 None:intergenic
GTTCCGTCCACACCTTGATA+GGG 0.517808 4.1:+26445426 None:intergenic
ACAATGGATTGGAGGAAGAA+AGG 0.520772 4.1:-26445785 MS.gene006682:CDS
GGGGTGAGGAAGGATACATT+AGG 0.521721 4.1:-26445128 MS.gene006682:CDS
AGGATACATTAGGATGCAAA+GGG 0.521998 4.1:-26445118 MS.gene006682:CDS
CAGCTATTGCGGCAACTGAA+GGG 0.522776 4.1:-26445608 MS.gene006682:CDS
GGATATGGCATTAACAATGA+TGG 0.525852 4.1:-26445192 MS.gene006682:CDS
TCTGTAGCCATTGATGCTAG+TGG 0.529031 4.1:-26445294 MS.gene006682:CDS
ATGTTTGACATTTAAGCCGT+AGG 0.532128 4.1:+26445045 None:intergenic
CTTGTTGATTGTGACACAAA+AGG 0.533495 4.1:-26445537 MS.gene006682:CDS
GATGCTGCAGAAGGACTTTG+TGG 0.533809 4.1:-26445090 MS.gene006682:CDS
GCTCGTGTTTCTCATACATA+GGG 0.536434 4.1:+26446068 None:intergenic
GGAACCGACTGGGGTGAGGA+AGG 0.537425 4.1:-26445138 MS.gene006682:CDS
AATAACAAATCATACAAACT+TGG 0.543607 4.1:-26445935 MS.gene006682:CDS
TGTATGAGAAACACGAGCAA+TGG 0.547122 4.1:-26446063 MS.gene006682:CDS
TGTGGTACTGAGTTGGATCA+TGG 0.551365 4.1:-26445228 MS.gene006682:CDS
TTCCATCCACAATGGATTGG+AGG 0.551446 4.1:-26445793 MS.gene006682:CDS
GTGTTCCATCCACAATGGAT+TGG 0.552593 4.1:-26445796 MS.gene006682:CDS
TGATGGAACCAAATATTGGT+TGG 0.554328 4.1:-26445175 MS.gene006682:CDS
TACCCAGTAATGGTAGCAGC+AGG 0.558279 4.1:+26445378 None:intergenic
GTGCGTTCTCATTGTTGGCA+GGG 0.560091 4.1:+26445346 None:intergenic
AAGGATACATTAGGATGCAA+AGG 0.562755 4.1:-26445119 MS.gene006682:CDS
GGATCATGGTGTTACTGCTG+TGG 0.569092 4.1:-26445214 MS.gene006682:CDS
AATGCAGGCATCTTATCCTA+CGG 0.576356 4.1:-26445061 MS.gene006682:CDS
TACCCCTATCAAGGTGTGGA+CGG 0.577316 4.1:-26445429 MS.gene006682:CDS
AACTCATGGGGAACCGACTG+GGG 0.580555 4.1:-26445147 MS.gene006682:CDS
TGGTTCATGTGGTACTGAGT+TGG 0.586183 4.1:-26445235 MS.gene006682:CDS
GATCATGGTGTTACTGCTGT+GGG 0.587872 4.1:-26445213 MS.gene006682:CDS
GGTGTAGACCAAGGTTGTGA+GGG 0.591635 4.1:-26445516 MS.gene006682:CDS
TAAGACCACCCTCACAACCT+TGG 0.592405 4.1:+26445508 None:intergenic
AGGTGTAGACCAAGGTTGTG+AGG 0.594253 4.1:-26445517 MS.gene006682:CDS
GTAGACCAAGGTTGTGAGGG+TGG 0.595756 4.1:-26445513 MS.gene006682:CDS
TTCCGTCCACACCTTGATAG+GGG 0.599701 4.1:+26445427 None:intergenic
GAGGCCGAATACCCCTATCA+AGG 0.601939 4.1:-26445438 MS.gene006682:CDS
GACATCCTCATACCCAGTAA+TGG 0.602674 4.1:+26445368 None:intergenic
GATCCTTATACACTTTGCCA+TGG 0.603597 4.1:+26446032 None:intergenic
ACATGTGCTCTTCAATCACC+AGG 0.611193 4.1:-26445847 MS.gene006682:CDS
TGAGAAACACGAGCAATGGA+TGG 0.613416 4.1:-26446059 MS.gene006682:CDS
GGTGTCTTCACTGGTTCATG+TGG 0.623688 4.1:-26445246 MS.gene006682:CDS
GTCATCTTGTAGAGTGCGAG+AGG 0.637765 4.1:+26446090 None:intergenic
AGGGGCGTAGATGCTGCAGA+AGG 0.641062 4.1:-26445099 MS.gene006682:CDS
GTATCCTTCCTCACCCCAGT+CGG 0.644090 4.1:+26445134 None:intergenic
ATATGAACTCCTCATTGGTG+AGG 0.644741 4.1:+26445894 None:intergenic
TGCGAGTGTTCCATCCACAA+TGG 0.653708 4.1:-26445801 MS.gene006682:CDS
ATTTGCAGACCTCACCAATG+AGG 0.664553 4.1:-26445903 MS.gene006682:CDS
CGAATACCCCTATCAAGGTG+TGG 0.692068 4.1:-26445433 MS.gene006682:CDS
GAGCAATGGATGGCTCACCA+TGG 0.701771 4.1:-26446049 MS.gene006682:CDS
GACACAAAAGGTGTAGACCA+AGG 0.707755 4.1:-26445525 MS.gene006682:CDS
AAATCATGGAATCAGTACTG+AGG 0.718091 4.1:-26445457 MS.gene006682:CDS
GGATACATTAGGATGCAAAG+GGG 0.764149 4.1:-26445117 MS.gene006682:CDS
GGATTGGAGGAAGAAAGGAG+CGG 0.765865 4.1:-26445780 MS.gene006682:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGATATAATTGATTAT+TGG + chr4.1:26445066-26445085 None:intergenic 10.0%
!! TCAATTATATCATATTTCAT+TGG - chr4.1:26445070-26445089 MS.gene006682:CDS 15.0%
!! TTGAATTTCTTATAGATATA+TGG - chr4.1:26445522-26445541 MS.gene006682:CDS 15.0%
!!! AAAGGCATATTTAAAATAAT+TGG + chr4.1:26445572-26445591 None:intergenic 15.0%
!!! TATTTTAAATATGCCTTTAT+AGG - chr4.1:26445574-26445593 MS.gene006682:CDS 15.0%
!! AAATTCATCATTCAAAATCA+TGG - chr4.1:26445751-26445770 MS.gene006682:CDS 20.0%
!! AATAACAAATCATACAAACT+TGG - chr4.1:26445287-26445306 MS.gene006682:CDS 20.0%
! ATATTTAAAAGTAGTTGTCC+TGG + chr4.1:26445396-26445415 None:intergenic 25.0%
! ATCATCCAAAAATAGATTCA+AGG - chr4.1:26445349-26445368 MS.gene006682:CDS 25.0%
! TCATCCAAAAATAGATTCAA+GGG - chr4.1:26445350-26445369 MS.gene006682:CDS 25.0%
!! ACTTTTGTAAAACTGAAAGT+CGG + chr4.1:26445956-26445975 None:intergenic 25.0%
!! GTTTTTAACCAACCAATATT+TGG + chr4.1:26446058-26446077 None:intergenic 25.0%
!!! TAAGTTAAGTACTGGAAAAT+TGG - chr4.1:26445642-26445661 MS.gene006682:intron 25.0%
ACAATCAACAAGTTCTTGTT+CGG + chr4.1:26445677-26445696 None:intergenic 30.0%
CATCCAAAAATAGATTCAAG+GGG - chr4.1:26445351-26445370 MS.gene006682:CDS 30.0%
! ACAATGATGGAACCAAATAT+TGG - chr4.1:26446043-26446062 MS.gene006682:CDS 30.0%
! ACATTGACCTTGATTTTTCA+CGG + chr4.1:26445474-26445493 None:intergenic 30.0%
! ATTGGTTGGTTAAAAACTCA+TGG - chr4.1:26446061-26446080 MS.gene006682:CDS 30.0%
! GACTTTCAGTTTTACAAAAG+TGG - chr4.1:26445955-26445974 MS.gene006682:CDS 30.0%
! TTGGTTGGTTAAAAACTCAT+GGG - chr4.1:26446062-26446081 MS.gene006682:CDS 30.0%
!! TTTGTTATTGTCAGCATTGT+TGG + chr4.1:26445276-26445295 None:intergenic 30.0%
AAATCATGGAATCAGTACTG+AGG - chr4.1:26445765-26445784 MS.gene006682:CDS 35.0%
AAGGATACATTAGGATGCAA+AGG - chr4.1:26446103-26446122 MS.gene006682:CDS 35.0%
AGGATACATTAGGATGCAAA+GGG - chr4.1:26446104-26446123 MS.gene006682:CDS 35.0%
ATGCCTTTATAGGATGTTGT+TGG - chr4.1:26445584-26445603 MS.gene006682:CDS 35.0%
GGAACATGCAATGCTAATAA+AGG - chr4.1:26445814-26445833 MS.gene006682:CDS 35.0%
GGATATGGCATTAACAATGA+TGG - chr4.1:26446030-26446049 MS.gene006682:CDS 35.0%
GGGATTCATAAGTTAAGTAC+TGG - chr4.1:26445634-26445653 MS.gene006682:intron 35.0%
GTGAAAAATCAAGGTCAATG+TGG - chr4.1:26445473-26445492 MS.gene006682:CDS 35.0%
TGATGGAACCAAATATTGGT+TGG - chr4.1:26446047-26446066 MS.gene006682:CDS 35.0%
TGCCTTTATAGGATGTTGTT+GGG - chr4.1:26445585-26445604 MS.gene006682:CDS 35.0%
! CATTGACCTTGATTTTTCAC+GGG + chr4.1:26445473-26445492 None:intergenic 35.0%
! CTTGTTGATTGTGACACAAA+AGG - chr4.1:26445685-26445704 MS.gene006682:intron 35.0%
! TGGTTGGTTAAAAACTCATG+GGG - chr4.1:26446063-26446082 MS.gene006682:CDS 35.0%
!! ATTGGCATTGCTTTTATGCT+TGG - chr4.1:26445088-26445107 MS.gene006682:CDS 35.0%
!! TTGGCATTGCTTTTATGCTT+GGG - chr4.1:26445089-26445108 MS.gene006682:CDS 35.0%
AATGCAGGCATCTTATCCTA+CGG - chr4.1:26446161-26446180 MS.gene006682:CDS 40.0%
ATATGAACTCCTCATTGGTG+AGG + chr4.1:26445331-26445350 None:intergenic 40.0%
GATCCTTATACACTTTGCCA+TGG + chr4.1:26445193-26445212 None:intergenic 40.0%
GCATCAATGGCTACAGAAAT+TGG + chr4.1:26445925-26445944 None:intergenic 40.0%
GCTCGTGTTTCTCATACATA+GGG + chr4.1:26445157-26445176 None:intergenic 40.0%
GGATACATTAGGATGCAAAG+GGG - chr4.1:26446105-26446124 MS.gene006682:CDS 40.0%
GGATGATATGAACTCCTCAT+TGG + chr4.1:26445336-26445355 None:intergenic 40.0%
TACAAAAGTGGTGTCTTCAC+TGG - chr4.1:26445967-26445986 MS.gene006682:CDS 40.0%
TCACCATGGCAAAGTGTATA+AGG - chr4.1:26445187-26445206 MS.gene006682:CDS 40.0%
TGCTCGTGTTTCTCATACAT+AGG + chr4.1:26445158-26445177 None:intergenic 40.0%
TGTATGAGAAACACGAGCAA+TGG - chr4.1:26445159-26445178 MS.gene006682:CDS 40.0%
TTTCTTCCTCCAATCCATTG+TGG + chr4.1:26445438-26445457 None:intergenic 40.0%
! GCTTTTATGCTTGGGATTGT+GGG - chr4.1:26445097-26445116 MS.gene006682:CDS 40.0%
! GTGCCCCTTGAATCTATTTT+TGG + chr4.1:26445357-26445376 None:intergenic 40.0%
! TGCTTTTATGCTTGGGATTG+TGG - chr4.1:26445096-26445115 MS.gene006682:CDS 40.0%
!! ACAATGGATTGGAGGAAGAA+AGG - chr4.1:26445437-26445456 MS.gene006682:CDS 40.0%
ACATGTGCTCTTCAATCACC+AGG - chr4.1:26445375-26445394 MS.gene006682:CDS 45.0%
ACTTTGTGGCATTGCAATGC+AGG - chr4.1:26446146-26446165 MS.gene006682:CDS 45.0%
ATTTGCAGACCTCACCAATG+AGG - chr4.1:26445319-26445338 MS.gene006682:CDS 45.0%
CGCCCAACAACATCCTATAA+AGG + chr4.1:26445590-26445609 None:intergenic 45.0%
GACATCCTCATACCCAGTAA+TGG + chr4.1:26445857-26445876 None:intergenic 45.0%
GTGACACCCGTGAAAAATCA+AGG - chr4.1:26445464-26445483 MS.gene006682:CDS 45.0%
GTGTTCCATCCACAATGGAT+TGG - chr4.1:26445426-26445445 MS.gene006682:CDS 45.0%
TCTGTAGCCATTGATGCTAG+TGG - chr4.1:26445928-26445947 MS.gene006682:CDS 45.0%
TGAGAAACACGAGCAATGGA+TGG - chr4.1:26445163-26445182 MS.gene006682:CDS 45.0%
TGCTACCATTACTGGGTATG+AGG - chr4.1:26445849-26445868 MS.gene006682:CDS 45.0%
TTCCTCCAATCCATTGTGGA+TGG + chr4.1:26445434-26445453 None:intergenic 45.0%
TTGCAGTGCGTTCTCATTGT+TGG + chr4.1:26445884-26445903 None:intergenic 45.0%
! GACACAAAAGGTGTAGACCA+AGG - chr4.1:26445697-26445716 MS.gene006682:intron 45.0%
! TGGTTCATGTGGTACTGAGT+TGG - chr4.1:26445987-26446006 MS.gene006682:CDS 45.0%
! TGTGGTACTGAGTTGGATCA+TGG - chr4.1:26445994-26446013 MS.gene006682:CDS 45.0%
! TTGGGCGTTTTCAGCTATTG+CGG - chr4.1:26445603-26445622 MS.gene006682:CDS 45.0%
!! CACACCTTGATAGGGGTATT+CGG + chr4.1:26445791-26445810 None:intergenic 45.0%
!! GATCATGGTGTTACTGCTGT+GGG - chr4.1:26446009-26446028 MS.gene006682:CDS 45.0%
!! TTCCATCCACAATGGATTGG+AGG - chr4.1:26445429-26445448 MS.gene006682:CDS 45.0%
AAAACTCATGGGGAACCGAC+TGG - chr4.1:26446073-26446092 MS.gene006682:CDS 50.0%
AAACTCATGGGGAACCGACT+GGG - chr4.1:26446074-26446093 MS.gene006682:CDS 50.0%
AGGTGTAGACCAAGGTTGTG+AGG - chr4.1:26445705-26445724 MS.gene006682:intron 50.0%
AGTGCGTTCTCATTGTTGGC+AGG + chr4.1:26445880-26445899 None:intergenic 50.0%
CAGCTATTGCGGCAACTGAA+GGG - chr4.1:26445614-26445633 MS.gene006682:CDS 50.0%
CGAATACCCCTATCAAGGTG+TGG - chr4.1:26445789-26445808 MS.gene006682:CDS 50.0%
CTCCTGCTGCTACCATTACT+GGG - chr4.1:26445842-26445861 MS.gene006682:CDS 50.0%
GAACGCACTGCAAAAAGCAG+TGG - chr4.1:26445891-26445910 MS.gene006682:CDS 50.0%
GATGCTGCAGAAGGACTTTG+TGG - chr4.1:26446132-26446151 MS.gene006682:CDS 50.0%
GGATTGGAGGAAGAAAGGAG+CGG - chr4.1:26445442-26445461 MS.gene006682:CDS 50.0%
GGGGTGAGGAAGGATACATT+AGG - chr4.1:26446094-26446113 MS.gene006682:CDS 50.0%
GGTGTAGACCAAGGTTGTGA+GGG - chr4.1:26445706-26445725 MS.gene006682:intron 50.0%
GTCATCTTGTAGAGTGCGAG+AGG + chr4.1:26445135-26445154 None:intergenic 50.0%
GTCGGATCCACTAGCATCAA+TGG + chr4.1:26445938-26445957 None:intergenic 50.0%
GTGCGTTCTCATTGTTGGCA+GGG + chr4.1:26445879-26445898 None:intergenic 50.0%
GTTCCGTCCACACCTTGATA+GGG + chr4.1:26445799-26445818 None:intergenic 50.0%
TAAGACCACCCTCACAACCT+TGG + chr4.1:26445717-26445736 None:intergenic 50.0%
TACCCAGTAATGGTAGCAGC+AGG + chr4.1:26445847-26445866 None:intergenic 50.0%
TACCCCTATCAAGGTGTGGA+CGG - chr4.1:26445793-26445812 MS.gene006682:CDS 50.0%
TCAGCTATTGCGGCAACTGA+AGG - chr4.1:26445613-26445632 MS.gene006682:CDS 50.0%
TGCGAGTGTTCCATCCACAA+TGG - chr4.1:26445421-26445440 MS.gene006682:CDS 50.0%
TGTTCCGTCCACACCTTGAT+AGG + chr4.1:26445800-26445819 None:intergenic 50.0%
TTCCGTCCACACCTTGATAG+GGG + chr4.1:26445798-26445817 None:intergenic 50.0%
! AGGTTGTGAGGGTGGTCTTA+TGG - chr4.1:26445717-26445736 MS.gene006682:intron 50.0%
!! GGATCATGGTGTTACTGCTG+TGG - chr4.1:26446008-26446027 MS.gene006682:CDS 50.0%
!! GGTGTCTTCACTGGTTCATG+TGG - chr4.1:26445976-26445995 MS.gene006682:CDS 50.0%
!! GGTGTTACTGCTGTGGGATA+TGG - chr4.1:26446015-26446034 MS.gene006682:CDS 50.0%
AACTCATGGGGAACCGACTG+GGG - chr4.1:26446075-26446094 MS.gene006682:CDS 55.0%
GAGCAATGGATGGCTCACCA+TGG - chr4.1:26445173-26445192 MS.gene006682:CDS 55.0%
GAGGCCGAATACCCCTATCA+AGG - chr4.1:26445784-26445803 MS.gene006682:CDS 55.0%
GCTCCTGCTGCTACCATTAC+TGG - chr4.1:26445841-26445860 MS.gene006682:CDS 55.0%
GTAGACCAAGGTTGTGAGGG+TGG - chr4.1:26445709-26445728 MS.gene006682:intron 55.0%
GTATCCTTCCTCACCCCAGT+CGG + chr4.1:26446091-26446110 None:intergenic 55.0%
! AGGGGCGTAGATGCTGCAGA+AGG - chr4.1:26446123-26446142 MS.gene006682:CDS 60.0%
ATGGGGAACCGACTGGGGTG+AGG - chr4.1:26446080-26446099 MS.gene006682:CDS 65.0%
GGAACCGACTGGGGTGAGGA+AGG - chr4.1:26446084-26446103 MS.gene006682:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 26445056 26446188 26445056 ID=MS.gene006682
chr4.1 mRNA 26445056 26446188 26445056 ID=MS.gene006682.t1;Parent=MS.gene006682
chr4.1 exon 26445750 26446188 26445750 ID=MS.gene006682.t1.exon1;Parent=MS.gene006682.t1
chr4.1 CDS 26445750 26446188 26445750 ID=cds.MS.gene006682.t1;Parent=MS.gene006682.t1
chr4.1 exon 26445056 26445648 26445056 ID=MS.gene006682.t1.exon2;Parent=MS.gene006682.t1
chr4.1 CDS 26445056 26445648 26445056 ID=cds.MS.gene006682.t1;Parent=MS.gene006682.t1
Gene Sequence

>MS.gene006682

ATGGCTGCCAATAATCAATTATATCATATTTCATTGGCATTGCTTTTATGCTTGGGATTGTGGGCAATTCAAATCACCTCTCGCACTCTACAAGATGACCCTATGTATGAGAAACACGAGCAATGGATGGCTCACCATGGCAAAGTGTATAAGGATCTTCAAGAAAGAGAGAATCGTTTAAAGATATTCAAAGAAAATGTGAATTACATTGAAGCTTCCAACAATGCTGACAATAACAAATCATACAAACTTGGCATTAATCAATTTGCAGACCTCACCAATGAGGAGTTCATATCATCCAAAAATAGATTCAAGGGGCACATGTGCTCTTCAATCACCAGGACAACTACTTTTAAATATGAAAATGCGAGTGTTCCATCCACAATGGATTGGAGGAAGAAAGGAGCGGTGACACCCGTGAAAAATCAAGGTCAATGTGGTAAGTGTTTATCGAATAATTAATTCATTGAATTTCTTATAGATATATGGTCAAGTTTGAATTGTCATCTGACTCCAATTATTTTAAATATGCCTTTATAGGATGTTGTTGGGCGTTTTCAGCTATTGCGGCAACTGAAGGGATTCATAAGTTAAGTACTGGAAAATTGGTGTCTTTATCCGAACAAGAACTTGTTGATTGTGACACAAAAGGTGTAGACCAAGGTTGTGAGGGTGGTCTTATGGATGATGCTTTCAAATTCATCATTCAAAATCATGGAATCAGTACTGAGGCCGAATACCCCTATCAAGGTGTGGACGGAACATGCAATGCTAATAAAGGTTCAGCTCCTGCTGCTACCATTACTGGGTATGAGGATGTCCCTGCCAACAATGAGAACGCACTGCAAAAAGCAGTGGCTAATCAACCAATTTCTGTAGCCATTGATGCTAGTGGATCCGACTTTCAGTTTTACAAAAGTGGTGTCTTCACTGGTTCATGTGGTACTGAGTTGGATCATGGTGTTACTGCTGTGGGATATGGCATTAACAATGATGGAACCAAATATTGGTTGGTTAAAAACTCATGGGGAACCGACTGGGGTGAGGAAGGATACATTAGGATGCAAAGGGGCGTAGATGCTGCAGAAGGACTTTGTGGCATTGCAATGCAGGCATCTTATCCTACGGCTTAA
Protein sequence

>MS.gene006682.t1

MAANNQLYHISLALLLCLGLWAIQITSRTLQDDPMYEKHEQWMAHHGKVYKDLQERENRLKIFKENVNYIEASNNADNNKSYKLGINQFADLTNEEFISSKNRFKGHMCSSITRTTTFKYENASVPSTMDWRKKGAVTPVKNQGQCGCCWAFSAIAATEGIHKLSTGKLVSLSEQELVDCDTKGVDQGCEGGLMDDAFKFIIQNHGISTEAEYPYQGVDGTCNANKGSAPAATITGYEDVPANNENALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGINNDGTKYWLVKNSWGTDWGEEGYIRMQRGVDAAEGLCGIAMQASYPTA