AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026873

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026873.t1 MTR_2g075830 94.094 254 13 1 31 282 88 341 2.82e-179 497
MS.gene026873.t1 MTR_4g107930 74.088 274 65 3 13 282 74 345 4.19e-148 419
MS.gene026873.t1 MTR_5g022560 76.654 257 56 2 30 282 88 344 1.43e-146 414
MS.gene026873.t1 MTR_4g047610 72.727 275 67 4 13 282 72 343 8.51e-146 412
MS.gene026873.t1 MTR_4g079770 74.453 274 61 5 14 282 76 345 1.72e-145 412
MS.gene026873.t1 MTR_4g079470 74.453 274 61 5 14 282 76 345 1.78e-145 412
MS.gene026873.t1 MTR_4g079800 72.993 274 65 5 14 282 76 345 4.61e-143 406
MS.gene026873.t1 MTR_4g080700 62.308 260 89 3 31 282 82 340 7.22e-119 344
MS.gene026873.t1 MTR_4g080730 61.154 260 92 3 31 282 82 340 5.05e-116 337
MS.gene026873.t1 MTR_4g104370 55.208 288 110 6 10 280 57 342 1.53e-105 311
MS.gene026873.t1 MTR_3g075390 54.035 285 113 6 13 280 60 343 3.03e-102 303
MS.gene026873.t1 MTR_7g078600 61.504 226 80 4 58 280 115 336 4.37e-101 299
MS.gene026873.t1 MTR_4g080360 56.693 254 101 4 36 282 91 342 4.98e-101 299
MS.gene026873.t1 MTR_7g079230 58.798 233 91 3 50 280 105 334 1.35e-96 288
MS.gene026873.t1 MTR_2g083930 56.828 227 96 1 56 280 116 342 1.67e-96 288
MS.gene026873.t1 MTR_4g079440 54.941 253 110 3 30 280 93 343 2.21e-96 287
MS.gene026873.t1 MTR_3g116080 60.173 231 87 4 53 281 120 347 3.65e-95 284
MS.gene026873.t1 MTR_1g107550 57.724 246 98 5 39 280 111 354 3.80e-94 286
MS.gene026873.t1 MTR_2g090890 53.012 249 110 4 36 281 92 336 2.40e-92 276
MS.gene026873.t1 MTR_1g018840 55.833 240 101 4 44 280 123 360 9.57e-90 274
MS.gene026873.t1 MTR_1g018840 55.833 240 101 4 44 280 123 360 9.78e-90 275
MS.gene026873.t1 MTR_4g094918 57.339 218 90 3 66 281 125 341 5.88e-88 266
MS.gene026873.t1 MTR_3g102220 58.716 218 85 4 66 280 145 360 6.36e-87 267
MS.gene026873.t1 MTR_3g112430 52.091 263 108 5 31 281 85 341 5.98e-86 264
MS.gene026873.t1 MTR_2g015850 49.805 257 119 6 30 280 110 362 6.13e-74 231
MS.gene026873.t1 MTR_2g016270 45.387 271 126 7 15 281 88 340 1.99e-71 227
MS.gene026873.t1 MTR_2g016180 44.928 276 135 7 15 281 88 355 2.08e-71 228
MS.gene026873.t1 MTR_1g023810 44.828 232 117 7 53 279 36 261 2.61e-61 195
MS.gene026873.t1 MTR_7g078630 50.754 199 81 4 56 241 756 950 2.85e-58 200
MS.gene026873.t1 MTR_2g067900 45.318 267 122 11 30 279 64 323 1.37e-57 187
MS.gene026873.t1 MTR_4g125300 41.264 269 138 9 26 281 87 348 1.75e-54 180
MS.gene026873.t1 MTR_4g065077 43.805 226 109 8 65 281 140 356 3.29e-54 180
MS.gene026873.t1 MTR_8g086470 43.805 226 109 8 65 281 140 356 3.29e-54 180
MS.gene026873.t1 MTR_1g023210 38.550 262 128 8 32 273 104 352 4.63e-54 179
MS.gene026873.t1 MTR_2g015920 41.667 240 120 10 49 273 116 350 6.38e-54 179
MS.gene026873.t1 MTR_4g071060 44.000 225 104 6 65 273 130 348 7.28e-54 179
MS.gene026873.t1 MTR_2g064475 45.575 226 104 11 66 281 135 351 2.68e-51 172
MS.gene026873.t1 MTR_2g067560 47.964 221 93 12 66 276 116 324 2.01e-49 175
MS.gene026873.t1 MTR_2g015930 40.654 214 114 8 67 273 143 350 4.62e-47 161
MS.gene026873.t1 MTR_1g094135 39.655 232 110 8 62 273 137 358 1.35e-45 158
MS.gene026873.t1 MTR_7g078580 64.286 98 32 2 185 280 4 100 3.09e-40 136
MS.gene026873.t1 MTR_2g066980 37.387 222 96 8 66 281 134 318 7.17e-40 141
MS.gene026873.t1 MTR_4g072790 39.720 214 93 8 65 273 130 312 3.70e-37 134
MS.gene026873.t1 MTR_7g079130 65.556 90 29 1 193 280 7 96 3.33e-36 125
MS.gene026873.t1 MTR_4g073660 38.991 218 93 8 65 273 129 315 1.39e-35 130
MS.gene026873.t1 MTR_4g072340 38.991 218 93 8 65 273 130 316 1.40e-35 130
MS.gene026873.t1 MTR_2g015840 56.250 112 46 2 172 281 161 271 6.19e-35 127
MS.gene026873.t1 MTR_2g015840 44.304 79 34 2 30 101 81 156 3.94e-13 68.2
MS.gene026873.t1 MTR_1g025450 38.095 189 88 8 53 234 42 208 3.73e-32 124
MS.gene026873.t1 MTR_7g078310 49.541 109 53 1 53 159 139 247 1.60e-29 112
MS.gene026873.t1 MTR_2g016380 43.966 116 60 3 170 281 21 135 1.12e-25 102
MS.gene026873.t1 MTR_3g029560 29.384 211 121 6 77 281 49 237 5.26e-23 95.1
MS.gene026873.t1 MTR_8g087510 43.182 132 68 5 107 236 23 149 1.49e-20 86.7
MS.gene026873.t1 MTR_7g111060 29.778 225 128 7 72 273 113 330 2.07e-20 90.1
MS.gene026873.t1 MTR_7g111050 30.932 236 133 9 61 273 99 327 1.87e-19 87.4
MS.gene026873.t1 MTR_2g047960 47.059 85 44 1 75 159 7 90 3.23e-17 75.5
MS.gene026873.t1 MTR_7g079260 52.778 72 16 2 209 278 7 62 1.56e-16 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026873.t1 AT5G50260 63.717 226 79 2 57 280 117 341 1.43e-104 309
MS.gene026873.t1 AT5G45890 56.274 263 104 4 28 281 85 345 9.25e-103 303
MS.gene026873.t1 AT3G48340 58.678 242 98 2 40 280 102 342 1.66e-101 301
MS.gene026873.t1 AT3G48350 53.047 279 114 4 19 281 66 343 5.71e-97 290
MS.gene026873.t1 AT4G36880 62.443 221 80 3 62 280 141 360 6.20e-96 287
MS.gene026873.t1 AT4G35350 57.778 225 93 2 58 281 129 352 2.94e-94 282
MS.gene026873.t1 AT1G06260 62.385 218 78 3 65 280 126 341 7.27e-94 281
MS.gene026873.t1 AT1G47128 56.466 232 97 4 52 280 121 351 4.70e-92 280
MS.gene026873.t1 AT5G43060 60.185 216 84 2 66 280 138 352 5.94e-92 280
MS.gene026873.t1 AT1G20850 56.769 229 97 2 54 281 126 353 6.38e-92 276
MS.gene026873.t1 AT2G27420 51.408 284 120 5 13 282 69 348 3.19e-91 274
MS.gene026873.t1 AT3G19390 53.101 258 116 4 28 281 89 345 3.50e-91 278
MS.gene026873.t1 AT3G49340 57.456 228 91 3 57 282 118 341 3.56e-90 271
MS.gene026873.t1 AT2G34080 51.321 265 115 6 28 282 85 345 8.56e-89 268
MS.gene026873.t1 AT3G19400 51.351 259 121 4 28 281 90 348 1.03e-88 268
MS.gene026873.t1 AT4G23520 55.708 219 90 5 66 280 133 348 5.48e-85 258
MS.gene026873.t1 AT1G09850 57.143 217 89 3 66 280 118 332 1.40e-83 258
MS.gene026873.t1 AT4G11310 53.540 226 99 4 59 280 128 351 4.26e-83 254
MS.gene026873.t1 AT1G29090 50.187 267 117 7 28 282 93 355 8.44e-83 253
MS.gene026873.t1 AT1G29080 49.071 269 116 9 28 282 85 346 1.90e-79 244
MS.gene026873.t1 AT4G11320 47.773 247 113 4 36 280 126 358 4.59e-79 244
MS.gene026873.t1 AT4G11320 47.773 247 113 4 36 280 126 358 4.59e-79 244
MS.gene026873.t1 AT3G43960 49.345 229 108 6 58 280 119 345 2.62e-69 219
MS.gene026873.t1 AT4G35350 59.375 160 63 2 58 217 129 286 4.45e-65 205
MS.gene026873.t1 AT3G48350 50.704 213 90 3 19 217 66 277 6.24e-64 202
MS.gene026873.t1 AT1G29110 45.575 226 102 6 62 281 123 333 9.45e-60 193
MS.gene026873.t1 AT3G19400 49.485 194 94 3 28 217 90 283 2.05e-57 186
MS.gene026873.t1 AT3G45310 45.089 224 109 8 66 282 141 357 4.46e-55 182
MS.gene026873.t1 AT2G21430 43.556 225 105 6 65 273 131 349 3.79e-54 179
MS.gene026873.t1 AT5G60360 45.291 223 108 8 66 281 141 356 1.09e-53 178
MS.gene026873.t1 AT4G39090 43.556 225 105 6 65 273 134 352 1.07e-52 176
MS.gene026873.t1 AT3G45310 44.196 224 110 8 66 282 141 356 1.34e-52 175
MS.gene026873.t1 AT4G16190 39.300 257 133 6 33 273 109 358 9.61e-52 174
MS.gene026873.t1 AT5G60360 44.843 223 108 9 66 281 141 355 3.41e-51 172
MS.gene026873.t1 AT5G60360 43.836 219 113 7 66 277 141 356 7.44e-51 171
MS.gene026873.t1 AT3G54940 40.693 231 107 8 63 273 134 354 8.31e-47 160
MS.gene026873.t1 AT3G54940 42.361 144 75 2 63 199 134 276 5.39e-33 122
MS.gene026873.t1 AT1G02305 32.000 225 123 8 72 273 116 333 4.40e-22 94.7
MS.gene026873.t1 AT4G01610 30.672 238 133 9 66 277 103 334 7.59e-22 94.0
MS.gene026873.t1 AT4G01610 30.252 238 134 9 66 277 103 334 1.38e-20 90.5
MS.gene026873.t1 AT1G02300 30.476 210 120 8 85 273 146 350 1.04e-17 82.4

Find 76 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAAAAGTGGTGTCTTTAC+TGG 0.255221 2.4:-21548424 MS.gene026873:CDS
AGCTCTGCTGCTACCATTTC+CGG 0.255468 2.4:-21548550 MS.gene026873:CDS
TAAGTTAAGTACAGGAAATT+TGG 0.266307 2.4:-21548749 MS.gene026873:CDS
CGTTGCAAATCAACCGGTTT+CGG 0.305328 2.4:-21548482 MS.gene026873:CDS
ACAACATGGCAAAGTCTATA+AGG 0.357194 2.4:-21549190 MS.gene026873:CDS
CAATGCCTGCTCATCATTTG+TGG 0.376785 2.4:+21548513 None:intergenic
GGTGTAGCTGTTGTGGGTTA+TGG 0.384097 2.4:-21547341 MS.gene026873:intron
AATTATGCAGTTGGATAAGA+TGG 0.402123 2.4:+21547188 None:intergenic
AATATTTGTAGGATGTTGTT+GGG 0.406693 2.4:-21548806 MS.gene026873:intron
ATTGGTTGGTGAAGAATTCA+TGG 0.413527 2.4:-21547295 MS.gene026873:CDS
AGGTTGTGAAGGTGGTCTTA+TGG 0.416045 2.4:-21548674 MS.gene026873:CDS
TACCCTTATCAAGGTGTTGA+TGG 0.417342 2.4:-21548598 MS.gene026873:CDS
GCATCAATGGCTACCGAAAC+CGG 0.418768 2.4:+21548469 None:intergenic
TAATATTTGTAGGATGTTGT+TGG 0.420839 2.4:-21548807 MS.gene026873:intron
GACATTCTCGTACCCGGAAA+TGG 0.421059 2.4:+21548537 None:intergenic
CCCCTGGGTGTAGCTGTTGT+GGG 0.432312 2.4:-21547347 MS.gene026873:intron
GAATTCATGGGGAACTCAAT+GGG 0.434741 2.4:-21547282 MS.gene026873:CDS
GGGGTGAAGAAGGGTACATT+AGG 0.434781 2.4:-21547262 MS.gene026873:CDS
GCTCTGCTGCTACCATTTCC+GGG 0.441657 2.4:-21548549 MS.gene026873:CDS
TTCCATCAACACCTTGATAA+GGG 0.450919 2.4:+21548596 None:intergenic
GCTGTTGTGGGTTATGGTGT+TGG 0.453735 2.4:-21547335 MS.gene026873:CDS
AATGCCTGCTCATCATTTGT+GGG 0.454729 2.4:+21548514 None:intergenic
AAAAGCCGTTGCAAATCAAC+CGG 0.459968 2.4:-21548488 MS.gene026873:CDS
GTCAGAGCCACTAGCATCAA+TGG 0.460761 2.4:+21548456 None:intergenic
GGAACTCAATGGGGTGAAGA+AGG 0.462980 2.4:-21547272 MS.gene026873:CDS
GTTCCATCAACACCTTGATA+AGG 0.464383 2.4:+21548595 None:intergenic
TGCTCATGCCTCTCATACAT+AGG 0.466056 2.4:+21549222 None:intergenic
AAATCTTATAATTATGCAGT+TGG 0.468251 2.4:+21547179 None:intergenic
GCAGCTAGAAACAAATTCAA+TGG 0.472406 2.4:-21549030 MS.gene026873:CDS
ACTTAACTTATGAATTCCTT+CGG 0.480238 2.4:+21548762 None:intergenic
GCCCCTGGGTGTAGCTGTTG+TGG 0.481622 2.4:-21547348 MS.gene026873:intron
CATTAGGATGCAAAGGGGTG+TGG 0.492848 2.4:-21547246 MS.gene026873:CDS
AGAATTCATGGGGAACTCAA+TGG 0.494609 2.4:-21547283 MS.gene026873:CDS
TACCATCTGCAGTGGATTGG+AGG 0.508009 2.4:-21548954 MS.gene026873:CDS
TTCTAGCTGCTATAAACTCA+TGG 0.510437 2.4:+21549043 None:intergenic
GGAATTCATAAGTTAAGTAC+AGG 0.513328 2.4:-21548757 MS.gene026873:CDS
ACATGCAATAAAACTGAAGT+AGG 0.516912 2.4:-21548574 MS.gene026873:CDS
ATCTGCAGTGGATTGGAGGC+AGG 0.519791 2.4:-21548950 MS.gene026873:CDS
TGTCCCCACAAATGATGAGC+AGG 0.522234 2.4:-21548518 MS.gene026873:CDS
AGGGTACATTAGGATGCAAA+GGG 0.524704 2.4:-21547252 MS.gene026873:CDS
TGTGGGGACATTCTCGTACC+CGG 0.543427 2.4:+21548531 None:intergenic
AAATTCAATGGATATTTGCA+TGG 0.546885 2.4:-21549018 MS.gene026873:CDS
AAGGGTACATTAGGATGCAA+AGG 0.551462 2.4:-21547253 MS.gene026873:CDS
TAATGGACTTAGCACTGAAG+TGG 0.556033 2.4:-21548623 MS.gene026873:CDS
TTGGTTGGTGAAGAATTCAT+GGG 0.557529 2.4:-21547294 MS.gene026873:CDS
CGAAACCGGTTGATTTGCAA+CGG 0.558104 2.4:+21548483 None:intergenic
TCGGCTGTTGCATCAACCGA+AGG 0.561296 2.4:-21548778 MS.gene026873:CDS
TGTGGAACTGAGTTGGATCA+TGG 0.564256 2.4:-21548397 MS.gene026873:intron
GAACTCAATGGGGTGAAGAA+GGG 0.565034 2.4:-21547271 MS.gene026873:CDS
GAAGTGGAATACCCTTATCA+AGG 0.568429 2.4:-21548607 MS.gene026873:CDS
GCTCATGCCTCTCATACATA+GGG 0.569140 2.4:+21549223 None:intergenic
GTGAAGATGAGACTGAGTAT+TGG 0.569553 2.4:-21547313 MS.gene026873:CDS
ATGTACCATCTGCAGTGGAT+TGG 0.570020 2.4:-21548957 MS.gene026873:CDS
TCGGTAGCCATTGATGCTAG+TGG 0.574292 2.4:-21548463 MS.gene026873:CDS
TGCCTCCAATCCACTGCAGA+TGG 0.579586 2.4:+21548952 None:intergenic
TAAGACCACCTTCACAACCT+TGG 0.583050 2.4:+21548677 None:intergenic
TCTATAAGGATGCACATGAA+AGG 0.584380 2.4:-21549176 MS.gene026873:CDS
TGGTTCATGTGGAACTGAGT+TGG 0.588339 2.4:-21548404 MS.gene026873:CDS
AGGGGTGTGGATGCTTCAGA+AGG 0.605030 2.4:-21547233 MS.gene026873:CDS
TGTATGAGAGGCATGAGCAA+TGG 0.614507 2.4:-21549218 MS.gene026873:CDS
GCAGTGGATTGGAGGCAGGA+AGG 0.619784 2.4:-21548946 MS.gene026873:CDS
AGATGAGACTGAGTATTGGT+TGG 0.624949 2.4:-21547309 MS.gene026873:CDS
AAAATGACCCTATGTATGAG+AGG 0.630864 2.4:-21549230 MS.gene026873:CDS
GAAGACCAAGGTTGTGAAGG+TGG 0.632713 2.4:-21548682 MS.gene026873:CDS
GGTGTCTTTACTGGTTCATG+TGG 0.637602 2.4:-21548415 MS.gene026873:CDS
GGTGAAGACCAAGGTTGTGA+AGG 0.642150 2.4:-21548685 MS.gene026873:CDS
GATGCTTCAGAAGGAATATG+TGG 0.650231 2.4:-21547224 MS.gene026873:CDS
CTTGTTGATTGTGACACAAG+TGG 0.664457 2.4:-21548706 MS.gene026873:CDS
AATTCATGGGGAACTCAATG+GGG 0.670782 2.4:-21547281 MS.gene026873:CDS
TAGTGATGTACCATCTGCAG+TGG 0.678103 2.4:-21548962 MS.gene026873:CDS
TGAGAGGCATGAGCAATGGA+TGG 0.686001 2.4:-21549214 MS.gene026873:CDS
ATGCCTGCTCATCATTTGTG+GGG 0.688383 2.4:+21548515 None:intergenic
TGGTTGGTGAAGAATTCATG+GGG 0.692894 2.4:-21547293 MS.gene026873:CDS
GAGCAATGGATGGTACAACA+TGG 0.707448 2.4:-21549204 MS.gene026873:CDS
GACACAAGTGGTGAAGACCA+AGG 0.761833 2.4:-21548694 MS.gene026873:CDS
GGGTACATTAGGATGCAAAG+GGG 0.770853 2.4:-21547251 MS.gene026873:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAAAAAAAAAAAAAGG+GGG + chr2.4:21548122-21548141 None:intergenic 10.0%
!! GAATTCATTATAAAAAATAA+TGG - chr2.4:21547777-21547796 MS.gene026873:intron 10.0%
!!! TTAAGTTTTTAAAATTGTTA+GGG - chr2.4:21548516-21548535 MS.gene026873:CDS 10.0%
!!! TTTAAGTTTTTAAAATTGTT+AGG - chr2.4:21548515-21548534 MS.gene026873:CDS 10.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr2.4:21548099-21548118 None:intergenic 100.0%
!! AAAAACTTAAAACAATCAAT+TGG + chr2.4:21548506-21548525 None:intergenic 15.0%
!! AATTTCTAATTAATAAGTTG+TGG - chr2.4:21548666-21548685 MS.gene026873:CDS 15.0%
!! GGAAATAAATCTTATAAATT+AGG - chr2.4:21547324-21547343 MS.gene026873:CDS 15.0%
!! TCAACAATTAATGATATATA+TGG - chr2.4:21548190-21548209 MS.gene026873:intron 15.0%
!! TTTAAAAAAAAAAAAAAGGG+GGG + chr2.4:21548121-21548140 None:intergenic 15.0%
!!! AACTAAATTTTACTATTACA+AGG + chr2.4:21547584-21547603 None:intergenic 15.0%
!! AATTCGACTAATTAATTGTT+AGG - chr2.4:21548595-21548614 MS.gene026873:CDS 20.0%
!! AGTAACCTATGTAATAAAAT+GGG - chr2.4:21548816-21548835 MS.gene026873:intron 20.0%
!! AGTTGTGATTTAATATTTGT+AGG - chr2.4:21547600-21547619 MS.gene026873:intron 20.0%
!! ATTCGACTAATTAATTGTTA+GGG - chr2.4:21548596-21548615 MS.gene026873:CDS 20.0%
!! TTAAAAAAAAAAAAAAGGGG+GGG + chr2.4:21548120-21548139 None:intergenic 20.0%
!! TTTATATAACAATCAAGATG+TGG - chr2.4:21547544-21547563 MS.gene026873:intron 20.0%
!! TTTCTATATAAGTTACAAGA+CGG + chr2.4:21548860-21548879 None:intergenic 20.0%
!!! GTTTTATATTGATTTTGAGT+AGG - chr2.4:21548572-21548591 MS.gene026873:CDS 20.0%
! AAATTCAATGGATATTTGCA+TGG - chr2.4:21547399-21547418 MS.gene026873:intron 25.0%
! AATATTTGTAGGATGTTGTT+GGG - chr2.4:21547611-21547630 MS.gene026873:intron 25.0%
! ACTTAACTTATGAATTCCTT+CGG + chr2.4:21547658-21547677 None:intergenic 25.0%
! ATTGAATCTTTCAACAATGT+TGG - chr2.4:21547303-21547322 MS.gene026873:CDS 25.0%
! ATTGCAATTTAAGAGAGAAA+GGG + chr2.4:21548384-21548403 None:intergenic 25.0%
! CTTCAAAAGTTTATAAACCA+TGG + chr2.4:21548727-21548746 None:intergenic 25.0%
! GAGTAACCTATGTAATAAAA+TGG - chr2.4:21548815-21548834 MS.gene026873:intron 25.0%
! TAAAAAAAAAAAAAAGGGGG+GGG + chr2.4:21548119-21548138 None:intergenic 25.0%
! TAAGCAAAAGTTTACACTTT+AGG - chr2.4:21548162-21548181 MS.gene026873:intron 25.0%
! TAAGTTAAGTACAGGAAATT+TGG - chr2.4:21547668-21547687 MS.gene026873:intron 25.0%
! TAATATTTGTAGGATGTTGT+TGG - chr2.4:21547610-21547629 MS.gene026873:intron 25.0%
!! AAATTTGAAATTGAGAGAGA+TGG + chr2.4:21548426-21548445 None:intergenic 25.0%
!! ATATATATGGTCTTTGTTGT+AGG - chr2.4:21548203-21548222 MS.gene026873:intron 25.0%
!! GACTTTCAATTTTACAAAAG+TGG - chr2.4:21547981-21548000 MS.gene026873:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG - chr2.4:21548264-21548283 MS.gene026873:intron 25.0%
!!! GGTTTATAAACTTTTGAAGT+TGG - chr2.4:21548728-21548747 MS.gene026873:CDS 25.0%
!!! TTTAGCTCATTTTCAACAAA+TGG - chr2.4:21548914-21548933 MS.gene026873:CDS 25.0%
!!! TTTTTTTTAAATACACCCTC+CGG - chr2.4:21548130-21548149 MS.gene026873:intron 25.0%
AAAAAAAAAAAAAAGGGGGG+GGG + chr2.4:21548118-21548137 None:intergenic 30.0%
AAACAATCAATTGGAGAATG+AGG + chr2.4:21548497-21548516 None:intergenic 30.0%
AACAATCAATTGGAGAATGA+GGG + chr2.4:21548496-21548515 None:intergenic 30.0%
AATAAGTTGTGGAAAACTTG+TGG - chr2.4:21548677-21548696 MS.gene026873:CDS 30.0%
ACATGCAATAAAACTGAAGT+AGG - chr2.4:21547843-21547862 MS.gene026873:intron 30.0%
ATCCAGACTTTAATTATACG+TGG + chr2.4:21549047-21549066 None:intergenic 30.0%
GATTGCAATTTAAGAGAGAA+AGG + chr2.4:21548385-21548404 None:intergenic 30.0%
GGAATTCATAAGTTAAGTAC+AGG - chr2.4:21547660-21547679 MS.gene026873:intron 30.0%
GTAAATGATTGATGTCACTA+AGG + chr2.4:21548545-21548564 None:intergenic 30.0%
GTAATAAAATGGGTGATGAA+TGG - chr2.4:21548826-21548845 MS.gene026873:intron 30.0%
TTGCAATTTAAGAGAGAAAG+GGG + chr2.4:21548383-21548402 None:intergenic 30.0%
! CATCACCCATTTTATTACAT+AGG + chr2.4:21548824-21548843 None:intergenic 30.0%
! CTCATTTTCAACAAATGGAT+AGG - chr2.4:21548919-21548938 MS.gene026873:CDS 30.0%
! CTTTTGCTTATAATAGTGAC+CGG + chr2.4:21548152-21548171 None:intergenic 30.0%
!! CTTTTGTTTAATATACCCTC+CGG + chr2.4:21548286-21548305 None:intergenic 30.0%
AAAAAAAAAAAAAGGGGGGG+GGG + chr2.4:21548117-21548136 None:intergenic 35.0%
ACAACATGGCAAAGTCTATA+AGG - chr2.4:21547227-21547246 MS.gene026873:CDS 35.0%
ACAATCAATTGGAGAATGAG+GGG + chr2.4:21548495-21548514 None:intergenic 35.0%
CAACACGAATTCATTTCGAA+AGG + chr2.4:21548635-21548654 None:intergenic 35.0%
CTCCACGTATAATTAAAGTC+TGG - chr2.4:21549042-21549061 MS.gene026873:CDS 35.0%
GCAGCTAGAAACAAATTCAA+TGG - chr2.4:21547387-21547406 MS.gene026873:intron 35.0%
GTGAAAAATCAAGGTCAATG+TGG - chr2.4:21547507-21547526 MS.gene026873:intron 35.0%
TACAAAAGTGGTGTCTTTAC+TGG - chr2.4:21547993-21548012 MS.gene026873:intron 35.0%
TCTATAAGGATGCACATGAA+AGG - chr2.4:21547241-21547260 MS.gene026873:CDS 35.0%
TTCAACAAATGGATAGGAGA+CGG - chr2.4:21548925-21548944 MS.gene026873:CDS 35.0%
TTCCATCAACACCTTGATAA+GGG + chr2.4:21547824-21547843 None:intergenic 35.0%
! CATGAAAGGCAAAAACGTTT+TGG - chr2.4:21547255-21547274 MS.gene026873:CDS 35.0%
! CATTGACCTTGATTTTTCAC+AGG + chr2.4:21547507-21547526 None:intergenic 35.0%
! TTCTAGCTGCTATAAACTCA+TGG + chr2.4:21547377-21547396 None:intergenic 35.0%
!! ATTGGTTGGTGAAGAATTCA+TGG - chr2.4:21549122-21549141 MS.gene026873:intron 35.0%
!! TTGGTTGGTGAAGAATTCAT+GGG - chr2.4:21549123-21549142 MS.gene026873:intron 35.0%
AAAAAAAAAAAAGGGGGGGG+GGG + chr2.4:21548116-21548135 None:intergenic 40.0%
AAAAGCCGTTGCAAATCAAC+CGG - chr2.4:21547929-21547948 MS.gene026873:intron 40.0%
AATGCCTGCTCATCATTTGT+GGG + chr2.4:21547906-21547925 None:intergenic 40.0%
AATTCATGGGGAACTCAATG+GGG - chr2.4:21549136-21549155 MS.gene026873:intron 40.0%
AGAATTCATGGGGAACTCAA+TGG - chr2.4:21549134-21549153 MS.gene026873:intron 40.0%
AGGGTACATTAGGATGCAAA+GGG - chr2.4:21549165-21549184 MS.gene026873:CDS 40.0%
ATTGCTTACCTTAGAGTGAG+AGG + chr2.4:21548469-21548488 None:intergenic 40.0%
CAATCAATTGGAGAATGAGG+GGG + chr2.4:21548494-21548513 None:intergenic 40.0%
GAAGTGGAATACCCTTATCA+AGG - chr2.4:21547810-21547829 MS.gene026873:intron 40.0%
GAATTCATGGGGAACTCAAT+GGG - chr2.4:21549135-21549154 MS.gene026873:intron 40.0%
GATGCTTCAGAAGGAATATG+TGG - chr2.4:21549193-21549212 MS.gene026873:CDS 40.0%
GTCACACCTGTGAAAAATCA+AGG - chr2.4:21547498-21547517 MS.gene026873:intron 40.0%
GTGAAGATGAGACTGAGTAT+TGG - chr2.4:21549104-21549123 MS.gene026873:intron 40.0%
GTTCCATCAACACCTTGATA+AGG + chr2.4:21547825-21547844 None:intergenic 40.0%
TAAGTGCAACACATTTGCCA+TGG - chr2.4:21548707-21548726 MS.gene026873:CDS 40.0%
TGCTTATAATAGTGACCGGA+GGG - chr2.4:21548268-21548287 MS.gene026873:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG + chr2.4:21548287-21548268 None:intergenic 40.0%
TGTTTAGTTACGGTACAGGA+AGG + chr2.4:21548358-21548377 None:intergenic 40.0%
TTACGGTACAGGAAGGAAAT+TGG + chr2.4:21548351-21548370 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr2.4:21548267-21548286 MS.gene026873:intron 40.0%
TTGCTTATAATAGTGACCGG+AGG + chr2.4:21548286-21548267 None:intergenic 40.0%
! AAGGGTACATTAGGATGCAA+AGG - chr2.4:21549164-21549183 MS.gene026873:CDS 40.0%
! CTTGTTGATTGTGACACAAG+TGG - chr2.4:21547711-21547730 MS.gene026873:intron 40.0%
!! AGATGAGACTGAGTATTGGT+TGG - chr2.4:21549108-21549127 MS.gene026873:intron 40.0%
!! TAATGGACTTAGCACTGAAG+TGG - chr2.4:21547794-21547813 MS.gene026873:intron 40.0%
!! TACCCTTATCAAGGTGTTGA+TGG - chr2.4:21547819-21547838 MS.gene026873:intron 40.0%
!! TGGTTGGTGAAGAATTCATG+GGG - chr2.4:21549124-21549143 MS.gene026873:intron 40.0%
AAAAAAAAAAAGGGGGGGGG+GGG + chr2.4:21548115-21548134 None:intergenic 45.0%
AACTCTCACCTCTCACTCTA+AGG - chr2.4:21548458-21548477 MS.gene026873:CDS 45.0%
AGAGGTGAGAGTTGAGAAGT+AGG + chr2.4:21548451-21548470 None:intergenic 45.0%
ATGCCTGCTCATCATTTGTG+GGG + chr2.4:21547905-21547924 None:intergenic 45.0%
ATGTACCATCTGCAGTGGAT+TGG - chr2.4:21547460-21547479 MS.gene026873:intron 45.0%
CAATGCCTGCTCATCATTTG+TGG + chr2.4:21547907-21547926 None:intergenic 45.0%
GAACTCAATGGGGTGAAGAA+GGG - chr2.4:21549146-21549165 MS.gene026873:CDS 45.0%
GAGCAATGGATGGTACAACA+TGG - chr2.4:21547213-21547232 MS.gene026873:CDS 45.0%
GATGCATGTAGGAGCAATGT+GGG - chr2.4:21548057-21548076 MS.gene026873:intron 45.0%
GCTCATGCCTCTCATACATA+GGG + chr2.4:21547197-21547216 None:intergenic 45.0%
GGGTACATTAGGATGCAAAG+GGG - chr2.4:21549166-21549185 MS.gene026873:CDS 45.0%
TAAGACCACCTTCACAACCT+TGG + chr2.4:21547743-21547762 None:intergenic 45.0%
TAGTGATGTACCATCTGCAG+TGG - chr2.4:21547455-21547474 MS.gene026873:intron 45.0%
TGCTCATGCCTCTCATACAT+AGG + chr2.4:21547198-21547217 None:intergenic 45.0%
TGGTTCATGTGGAACTGAGT+TGG - chr2.4:21548013-21548032 MS.gene026873:intron 45.0%
! AGGATGTTGTTGGGCGTTTT+CGG - chr2.4:21547620-21547639 MS.gene026873:intron 45.0%
! AGGTTGTGAAGGTGGTCTTA+TGG - chr2.4:21547743-21547762 MS.gene026873:intron 45.0%
! CGTTGCAAATCAACCGGTTT+CGG - chr2.4:21547935-21547954 MS.gene026873:intron 45.0%
! GAAAGGGGCGTGTTTAGTTA+CGG + chr2.4:21548368-21548387 None:intergenic 45.0%
! TGTATGAGAGGCATGAGCAA+TGG - chr2.4:21547199-21547218 MS.gene026873:CDS 45.0%
! TGTGGAACTGAGTTGGATCA+TGG - chr2.4:21548020-21548039 MS.gene026873:intron 45.0%
!! CGAAACCGGTTGATTTGCAA+CGG + chr2.4:21547937-21547956 None:intergenic 45.0%
!! GGTGTCTTTACTGGTTCATG+TGG - chr2.4:21548002-21548021 MS.gene026873:intron 45.0%
!! GTATTTAAAAAAAAAAAAAA+GGG + chr2.4:21548124-21548143 None:intergenic 5.0%
!! TATTTAAAAAAAAAAAAAAG+GGG + chr2.4:21548123-21548142 None:intergenic 5.0%
!! TGTATTTAAAAAAAAAAAAA+AGG + chr2.4:21548125-21548144 None:intergenic 5.0%
AAAAAAAAAAGGGGGGGGGG+GGG + chr2.4:21548114-21548133 None:intergenic 50.0%
AGCTCTGCTGCTACCATTTC+CGG - chr2.4:21547867-21547886 MS.gene026873:intron 50.0%
AGTTGGATCATGGTGTAGCC+AGG - chr2.4:21548030-21548049 MS.gene026873:intron 50.0%
CATTAGGATGCAAAGGGGTG+TGG - chr2.4:21549171-21549190 MS.gene026873:CDS 50.0%
GAAGACCAAGGTTGTGAAGG+TGG - chr2.4:21547735-21547754 MS.gene026873:intron 50.0%
GACACAAGTGGTGAAGACCA+AGG - chr2.4:21547723-21547742 MS.gene026873:intron 50.0%
GACATTCTCGTACCCGGAAA+TGG + chr2.4:21547883-21547902 None:intergenic 50.0%
GCATCAATGGCTACCGAAAC+CGG + chr2.4:21547951-21547970 None:intergenic 50.0%
GGAACTCAATGGGGTGAAGA+AGG - chr2.4:21549145-21549164 MS.gene026873:intron 50.0%
GGATGCATGTAGGAGCAATG+TGG - chr2.4:21548056-21548075 MS.gene026873:intron 50.0%
GGCGTGTTTAGTTACGGTAC+AGG + chr2.4:21548362-21548381 None:intergenic 50.0%
GGTGAAGACCAAGGTTGTGA+AGG - chr2.4:21547732-21547751 MS.gene026873:intron 50.0%
GTCAGAGCCACTAGCATCAA+TGG + chr2.4:21547964-21547983 None:intergenic 50.0%
GTTGGATCATGGTGTAGCCA+GGG - chr2.4:21548031-21548050 MS.gene026873:intron 50.0%
TCGGTAGCCATTGATGCTAG+TGG - chr2.4:21547954-21547973 MS.gene026873:intron 50.0%
TGTCCCCACAAATGATGAGC+AGG - chr2.4:21547899-21547918 MS.gene026873:intron 50.0%
TTGGATCATGGTGTAGCCAG+GGG - chr2.4:21548032-21548051 MS.gene026873:intron 50.0%
! GCTGTTGTGGGTTATGGTGT+TGG - chr2.4:21549082-21549101 MS.gene026873:intron 50.0%
! GGGGTGAAGAAGGGTACATT+AGG - chr2.4:21549155-21549174 MS.gene026873:CDS 50.0%
! GGTGTAGCTGTTGTGGGTTA+TGG - chr2.4:21549076-21549095 MS.gene026873:intron 50.0%
! TGAGAGGCATGAGCAATGGA+TGG - chr2.4:21547203-21547222 MS.gene026873:CDS 50.0%
!! GAAGTTGGCTTTGCTCCTGT+CGG - chr2.4:21548743-21548762 MS.gene026873:CDS 50.0%
!! TACCATCTGCAGTGGATTGG+AGG - chr2.4:21547463-21547482 MS.gene026873:intron 50.0%
AAAAAAAAAGGGGGGGGGGG+GGG + chr2.4:21548113-21548132 None:intergenic 55.0%
AACCCACAACAGCTACACCC+AGG + chr2.4:21549075-21549094 None:intergenic 55.0%
ACCCACAACAGCTACACCCA+GGG + chr2.4:21549074-21549093 None:intergenic 55.0%
CACTTGCAGTTGCTACCGAC+AGG + chr2.4:21548761-21548780 None:intergenic 55.0%
GCTCTGCTGCTACCATTTCC+GGG - chr2.4:21547868-21547887 MS.gene026873:intron 55.0%
TAAAGTCTGGATCCGCCCCT+GGG - chr2.4:21549055-21549074 MS.gene026873:intron 55.0%
TCGGCTGTTGCATCAACCGA+AGG - chr2.4:21547639-21547658 MS.gene026873:intron 55.0%
TGCCTCCAATCCACTGCAGA+TGG + chr2.4:21547468-21547487 None:intergenic 55.0%
TGTGGGGACATTCTCGTACC+CGG + chr2.4:21547889-21547908 None:intergenic 55.0%
TTAAAGTCTGGATCCGCCCC+TGG - chr2.4:21549054-21549073 MS.gene026873:intron 55.0%
! AGGGGTGTGGATGCTTCAGA+AGG - chr2.4:21549184-21549203 MS.gene026873:CDS 55.0%
!! ATCTGCAGTGGATTGGAGGC+AGG - chr2.4:21547467-21547486 MS.gene026873:intron 55.0%
AAAAAAAAGGGGGGGGGGGG+GGG + chr2.4:21548112-21548131 None:intergenic 60.0%
ACAACAGCTACACCCAGGGG+CGG + chr2.4:21549070-21549089 None:intergenic 60.0%
CCCACAACAGCTACACCCAG+GGG + chr2.4:21549073-21549092 None:intergenic 60.0%
CCCCTGGGTGTAGCTGTTGT+GGG - chr2.4:21549070-21549089 MS.gene026873:intron 60.0%
GATCATGGTGTAGCCAGGGG+CGG - chr2.4:21548035-21548054 MS.gene026873:intron 60.0%
!! GCAGTGGATTGGAGGCAGGA+AGG - chr2.4:21547471-21547490 MS.gene026873:intron 60.0%
AAAAAAAGGGGGGGGGGGGG+GGG + chr2.4:21548111-21548130 None:intergenic 65.0%
AGCCAGGGGCGGATGCATGT+AGG - chr2.4:21548046-21548065 MS.gene026873:intron 65.0%
CTCCTACATGCATCCGCCCC+TGG + chr2.4:21548051-21548070 None:intergenic 65.0%
GCCCCTGGGTGTAGCTGTTG+TGG - chr2.4:21549069-21549088 MS.gene026873:intron 65.0%
AAAAAAGGGGGGGGGGGGGG+GGG + chr2.4:21548110-21548129 None:intergenic 70.0%
AAAAAGGGGGGGGGGGGGGG+GGG + chr2.4:21548109-21548128 None:intergenic 75.0%
! AAAAGGGGGGGGGGGGGGGG+GGG + chr2.4:21548108-21548127 None:intergenic 80.0%
!! GGGGGGGGGGGGGAAGCATG+TGG + chr2.4:21548092-21548111 None:intergenic 80.0%
! AAAGGGGGGGGGGGGGGGGG+GGG + chr2.4:21548107-21548126 None:intergenic 85.0%
! AAGGGGGGGGGGGGGGGGGG+GGG + chr2.4:21548106-21548125 None:intergenic 90.0%
! AGGGGGGGGGGGGGGGGGGG+GGG + chr2.4:21548105-21548124 None:intergenic 95.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 21547190 21549249 21547190 ID=MS.gene026873
chr2.4 mRNA 21547190 21549249 21547190 ID=MS.gene026873.t1;Parent=MS.gene026873
chr2.4 exon 21549146 21549249 21549146 ID=MS.gene026873.t1.exon1;Parent=MS.gene026873.t1
chr2.4 CDS 21549146 21549249 21549146 ID=cds.MS.gene026873.t1;Parent=MS.gene026873.t1
chr2.4 exon 21548911 21549068 21548911 ID=MS.gene026873.t1.exon2;Parent=MS.gene026873.t1
chr2.4 CDS 21548911 21549068 21548911 ID=cds.MS.gene026873.t1;Parent=MS.gene026873.t1
chr2.4 exon 21548398 21548817 21548398 ID=MS.gene026873.t1.exon3;Parent=MS.gene026873.t1
chr2.4 CDS 21548398 21548817 21548398 ID=cds.MS.gene026873.t1;Parent=MS.gene026873.t1
chr2.4 exon 21547190 21547356 21547190 ID=MS.gene026873.t1.exon4;Parent=MS.gene026873.t1
chr2.4 CDS 21547190 21547356 21547190 ID=cds.MS.gene026873.t1;Parent=MS.gene026873.t1
Gene Sequence

>MS.gene026873

ATGACCCTATGTATGAGAGGCATGAGCAATGGATGGTACAACATGGCAAAGTCTATAAGGATGCACATGAAAGGCAAAAACGTTTTGGAATATTCAAAGAAAATGTAAATTACATTGAATCTTTCAACAATGTTGGAAATAAATCTTATAAATTAGGACTTAATCATTTTGCTGATCTTAGCAACCATGAGTTTATAGCAGCTAGAAACAAATTCAATGGATATTTGCATGGCTCAAGCATAACTACTTTTAAGTACAAAAATGTTAGTGATGTACCATCTGCAGTGGATTGGAGGCAGGAAGGAGCAGTCACACCTGTGAAAAATCAAGGTCAATGTGGTAAGTGTTTATTTATTTATATAACAATCAAGATGTGGTTTCTTATTTTTAACCTTGTAATAGTAAAATTTAGTTGTGATTTAATATTTGTAGGATGTTGTTGGGCGTTTTCGGCTGTTGCATCAACCGAAGGAATTCATAAGTTAAGTACAGGAAATTTGGTCTCTTTATCAGAACAAGAACTTGTTGATTGTGACACAAGTGGTGAAGACCAAGGTTGTGAAGGTGGTCTTATGGATGATGCTTTCGAATTCATTATAAAAAATAATGGACTTAGCACTGAAGTGGAATACCCTTATCAAGGTGTTGATGGAACATGCAATAAAACTGAAGTAGGCAGCTCTGCTGCTACCATTTCCGGGTACGAGAATGTCCCCACAAATGATGAGCAGGCATTGCAAAAAGCCGTTGCAAATCAACCGGTTTCGGTAGCCATTGATGCTAGTGGCTCTGACTTTCAATTTTACAAAAGTGGTGTCTTTACTGGTTCATGTGGAACTGAGTTGGATCATGGTGTAGCCAGGGGCGGATGCATGTAGGAGCAATGTGGGACTATAGCTCCACATGCTTCCCCCCCCCCCCCCCCCCCCCCCCCTTTTTTTTTTTTTTAAATACACCCTCCGGTCACTATTATAAGCAAAAGTTTACACTTTAGGTTCATTCAACAATTAATGATATATATGGTCTTTGTTGTAGGTCATATACATCATTCAATGAATGAATCTAAAATATAAATTTTTGCTTATAATAGTGACCGGAGGGTATATTAAACAAAAGACAACTGTGTGTCTCACTCTCACTCTCTTTTCATTTTCTATTCCAATTTCCTTCCTGTACCGTAACTAAACACGCCCCTTTCTCTCTTAAATTGCAATCTCTCTCAATCTCAGCTCTCCATCTCTCTCAATTTCAAATTTCTCCTACTTCTCAACTCTCACCTCTCACTCTAAGGTAAGCAATTTCCCCCTCATTCTCCAATTGATTGTTTTAAGTTTTTAAAATTGTTAGGGTTTCCTTAGTGACATCAATCATTTACTAAGAATGTTTTATATTGATTTTGAGTAGGAATTCGACTAATTAATTGTTAGGGATTCATGTTATTACCTTTCGAAATGAATTCGTGTTGTATGCAATGTTAATTTCTAATTAATAAGTTGTGGAAAACTTGTGGAAACAAATAAGTGCAACACATTTGCCATGGTTTATAAACTTTTGAAGTTGGCTTTGCTCCTGTCGGTAGCAACTGCAAGTGTTGAACGTGTTTTTTCAGCTATGAAAGTTGTGAAGAGTAACCTATGTAATAAAATGGGTGATGAATGGCTAAATGACCGTCTTGTAACTTATATAGAAAGAGATGTTCTTTTAACAATTAACAATGATGTTATTTTAGCTCATTTTCAACAAATGGATAGGAGACGGTTTTCTTTGTAATGTATTGCACTCTATAACAGAAAGAGATGTTAATTCTTATTTTTATTATAGTTTGCATTATTGTTTTTTTTAGAAATTTTAGCTCCACGTATAATTAAAGTCTGGATCCGCCCCTGGGTGTAGCTGTTGTGGGTTATGGTGTTGGTGAAGATGAGACTGAGTATTGGTTGGTGAAGAATTCATGGGGAACTCAATGGGGTGAAGAAGGGTACATTAGGATGCAAAGGGGTGTGGATGCTTCAGAAGGAATATGTGGTATTGCAATGCATCCATCTTATCCAACTGCATAA
Protein sequence

>MS.gene026873.t1

MTLCMRGMSNGWYNMAKSIRMHMKGKNVLEYSKKINHEFIAARNKFNGYLHGSSITTFKYKNVSDVPSAVDWRQEGAVTPVKNQGQCGCCWAFSAVASTEGIHKLSTGNLVSLSEQELVDCDTSGEDQGCEGGLMDDAFEFIIKNNGLSTEVEYPYQGVDGTCNKTEVGSSAATISGYENVPTNDEQALQKAVANQPVSVAIDASGSDFQFYKSGVFTGSCGTELDHAVVGYGVGEDETEYWLVKNSWGTQWGEEGYIRMQRGVDASEGICGIAMHPSYPTA