AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026874

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026874.t1 MTR_2g075830 95.114 307 13 1 1 305 35 341 0.0 592
MS.gene026874.t1 MTR_4g107930 77.419 310 65 3 1 305 36 345 8.53e-180 500
MS.gene026874.t1 MTR_4g079470 77.492 311 62 5 1 305 37 345 1.57e-178 496
MS.gene026874.t1 MTR_4g079770 77.492 311 62 5 1 305 37 345 1.70e-178 496
MS.gene026874.t1 MTR_5g022560 76.129 310 69 3 1 305 35 344 4.53e-178 495
MS.gene026874.t1 MTR_4g047610 76.527 311 66 4 1 305 34 343 6.78e-178 495
MS.gene026874.t1 MTR_4g079800 75.884 311 67 5 1 305 37 345 7.09e-176 490
MS.gene026874.t1 MTR_4g080700 64.052 306 108 2 1 305 36 340 5.18e-151 427
MS.gene026874.t1 MTR_4g080730 63.072 306 111 2 1 305 36 340 1.06e-147 418
MS.gene026874.t1 MTR_4g080360 57.929 309 121 4 4 305 36 342 8.42e-131 375
MS.gene026874.t1 MTR_2g083930 54.140 314 131 4 1 303 31 342 2.36e-123 357
MS.gene026874.t1 MTR_7g078600 56.209 306 124 5 1 303 38 336 7.26e-122 353
MS.gene026874.t1 MTR_4g104370 55.305 311 127 7 1 303 36 342 1.49e-118 345
MS.gene026874.t1 MTR_1g107550 55.627 311 128 6 1 303 46 354 6.76e-118 347
MS.gene026874.t1 MTR_3g075390 54.662 311 130 7 1 303 36 343 8.65e-118 343
MS.gene026874.t1 MTR_7g079230 55.082 305 130 4 1 303 35 334 8.83e-118 342
MS.gene026874.t1 MTR_4g079440 54.426 305 134 4 1 303 42 343 3.07e-115 336
MS.gene026874.t1 MTR_3g116080 54.808 312 126 7 1 304 43 347 2.24e-114 334
MS.gene026874.t1 MTR_2g090890 51.792 307 140 5 1 304 35 336 1.10e-111 327
MS.gene026874.t1 MTR_1g018840 52.885 312 128 7 5 303 55 360 5.51e-111 329
MS.gene026874.t1 MTR_1g018840 52.885 312 128 7 5 303 55 360 6.97e-111 330
MS.gene026874.t1 MTR_4g094918 50.485 309 142 6 5 304 35 341 2.67e-107 317
MS.gene026874.t1 MTR_3g112430 51.447 311 133 5 6 304 37 341 7.67e-106 316
MS.gene026874.t1 MTR_3g102220 51.911 314 130 10 5 303 53 360 1.30e-105 316
MS.gene026874.t1 MTR_2g015850 52.249 289 128 6 21 303 78 362 1.35e-92 279
MS.gene026874.t1 MTR_2g016270 45.779 308 145 7 3 304 49 340 1.85e-85 265
MS.gene026874.t1 MTR_2g016180 45.367 313 154 7 3 304 49 355 2.12e-85 265
MS.gene026874.t1 MTR_2g067900 47.152 316 143 11 4 302 15 323 3.05e-82 251
MS.gene026874.t1 MTR_2g064475 41.040 346 150 14 4 304 15 351 1.10e-73 230
MS.gene026874.t1 MTR_2g067560 45.342 322 138 15 4 299 15 324 1.39e-72 240
MS.gene026874.t1 MTR_7g078630 46.182 275 107 6 3 264 704 950 7.76e-70 233
MS.gene026874.t1 MTR_2g015920 40.836 311 156 12 3 296 51 350 7.55e-69 219
MS.gene026874.t1 MTR_4g125300 42.574 303 154 10 11 304 57 348 4.43e-67 213
MS.gene026874.t1 MTR_4g065077 40.125 319 167 11 1 304 47 356 2.21e-66 212
MS.gene026874.t1 MTR_2g066980 36.957 322 144 10 5 304 34 318 9.05e-64 204
MS.gene026874.t1 MTR_4g071060 39.404 302 160 7 11 296 54 348 2.77e-62 201
MS.gene026874.t1 MTR_2g015930 38.782 312 162 13 3 296 50 350 3.00e-62 201
MS.gene026874.t1 MTR_1g023810 44.828 232 117 7 76 302 36 261 1.48e-60 194
MS.gene026874.t1 MTR_1g023210 38.436 307 151 10 14 296 60 352 4.29e-60 196
MS.gene026874.t1 MTR_8g086470 38.291 316 161 11 1 304 63 356 1.52e-59 194
MS.gene026874.t1 MTR_1g094135 35.331 317 170 11 3 296 54 358 1.03e-49 169
MS.gene026874.t1 MTR_4g072790 36.027 297 141 12 11 296 54 312 3.87e-44 153
MS.gene026874.t1 MTR_4g072340 35.548 301 141 12 11 296 54 316 1.25e-42 149
MS.gene026874.t1 MTR_4g073660 35.548 301 141 12 11 296 53 315 1.80e-42 149
MS.gene026874.t1 MTR_7g078580 64.286 98 32 2 208 303 4 100 4.92e-40 136
MS.gene026874.t1 MTR_2g015840 51.163 129 53 2 3 124 31 156 2.87e-39 139
MS.gene026874.t1 MTR_2g015840 56.250 112 46 2 195 304 161 271 1.29e-34 127
MS.gene026874.t1 MTR_7g079130 64.835 91 30 1 215 303 6 96 5.52e-36 125
MS.gene026874.t1 MTR_1g025450 38.095 189 88 8 76 257 42 208 1.11e-31 124
MS.gene026874.t1 MTR_7g078310 44.286 140 71 3 45 182 113 247 5.67e-31 117
MS.gene026874.t1 MTR_2g016380 43.966 116 60 3 193 304 21 135 1.62e-25 102
MS.gene026874.t1 MTR_3g029560 29.384 211 121 6 100 304 49 237 7.70e-23 95.1
MS.gene026874.t1 MTR_8g087510 43.182 132 68 5 130 259 23 149 1.97e-20 87.0
MS.gene026874.t1 MTR_7g111060 29.778 225 128 7 95 296 113 330 6.78e-20 89.0
MS.gene026874.t1 MTR_7g111050 30.932 236 133 9 84 296 99 327 3.00e-19 87.0
MS.gene026874.t1 MTR_2g047960 47.059 85 44 1 98 182 7 90 5.07e-17 75.5
MS.gene026874.t1 MTR_7g079260 52.778 72 16 2 232 301 7 62 1.99e-16 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026874.t1 AT5G45890 55.096 314 129 5 1 304 34 345 3.01e-125 362
MS.gene026874.t1 AT5G50260 55.272 313 125 6 1 303 34 341 1.07e-119 348
MS.gene026874.t1 AT3G48340 54.221 308 129 5 5 303 38 342 1.67e-116 340
MS.gene026874.t1 AT2G34080 51.746 315 138 6 1 305 35 345 5.48e-114 333
MS.gene026874.t1 AT4G35350 52.581 310 137 5 1 304 47 352 3.39e-113 331
MS.gene026874.t1 AT2G27420 52.050 317 137 4 3 305 33 348 2.03e-112 329
MS.gene026874.t1 AT3G19390 52.273 308 139 5 1 304 42 345 7.48e-112 331
MS.gene026874.t1 AT3G49340 51.266 316 134 5 3 305 33 341 2.18e-111 326
MS.gene026874.t1 AT3G48350 53.355 313 134 7 1 304 34 343 7.64e-111 326
MS.gene026874.t1 AT4G36880 53.846 312 129 7 7 304 51 361 1.43e-110 325
MS.gene026874.t1 AT1G47128 52.117 307 134 8 5 303 50 351 1.33e-109 326
MS.gene026874.t1 AT1G06260 53.871 310 129 7 1 303 39 341 1.86e-109 322
MS.gene026874.t1 AT5G43060 53.571 308 129 8 5 303 50 352 1.90e-109 325
MS.gene026874.t1 AT3G19400 50.492 305 146 4 5 304 44 348 3.43e-109 322
MS.gene026874.t1 AT1G29090 50.794 315 139 7 3 305 45 355 9.11e-108 318
MS.gene026874.t1 AT1G20850 51.133 309 144 4 1 304 47 353 1.35e-107 317
MS.gene026874.t1 AT1G29080 50.476 315 135 9 5 305 39 346 3.45e-105 311
MS.gene026874.t1 AT1G09850 50.980 306 142 5 3 303 30 332 5.79e-103 308
MS.gene026874.t1 AT4G11310 49.675 308 139 7 6 303 50 351 1.08e-101 302
MS.gene026874.t1 AT4G23520 49.675 308 138 10 6 303 48 348 7.70e-101 300
MS.gene026874.t1 AT4G11320 47.869 305 149 5 6 303 57 358 3.49e-99 296
MS.gene026874.t1 AT4G11320 47.869 305 149 5 6 303 57 358 3.49e-99 296
MS.gene026874.t1 AT3G43960 46.278 309 152 8 5 303 41 345 6.28e-90 273
MS.gene026874.t1 AT1G29110 44.051 311 148 7 5 304 38 333 2.42e-84 257
MS.gene026874.t1 AT4G35350 52.245 245 107 5 1 240 47 286 7.25e-84 254
MS.gene026874.t1 AT3G48350 51.417 247 110 6 1 240 34 277 1.08e-77 239
MS.gene026874.t1 AT3G19400 48.750 240 119 3 5 240 44 283 1.80e-77 238
MS.gene026874.t1 AT3G45310 43.137 306 150 11 11 305 65 357 1.11e-69 220
MS.gene026874.t1 AT5G60360 43.607 305 148 11 11 304 65 356 1.60e-69 220
MS.gene026874.t1 AT3G45310 42.484 306 151 11 11 305 65 356 3.51e-67 214
MS.gene026874.t1 AT5G60360 43.279 305 148 12 11 304 65 355 5.29e-67 213
MS.gene026874.t1 AT5G60360 42.525 301 153 10 11 300 65 356 1.18e-66 213
MS.gene026874.t1 AT2G21430 38.889 306 157 9 11 296 54 349 1.90e-60 197
MS.gene026874.t1 AT4G39090 39.216 306 156 9 11 296 57 352 2.86e-60 196
MS.gene026874.t1 AT4G16190 38.436 307 159 8 11 296 61 358 1.64e-58 192
MS.gene026874.t1 AT3G54940 36.076 316 168 10 4 296 50 354 6.76e-52 175
MS.gene026874.t1 AT3G54940 36.000 225 132 4 8 222 54 276 3.53e-38 136
MS.gene026874.t1 AT1G02305 29.452 292 166 11 36 296 51 333 6.89e-22 94.7
MS.gene026874.t1 AT4G01610 28.136 295 174 11 36 300 48 334 1.12e-21 94.0
MS.gene026874.t1 AT4G01610 27.797 295 175 11 36 300 48 334 1.73e-20 90.9
MS.gene026874.t1 AT1G02300 30.476 210 120 8 108 296 146 350 2.55e-17 82.0
MS.gene026874.t1 AT2G22160 40.659 91 51 2 20 108 9 98 5.39e-15 70.1

Find 77 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATAAATCTTATAAATT+AGG 0.157228 2.4:-21561203 MS.gene026874:CDS
TACAAAAGTGGTGTCTTTAC+TGG 0.255221 2.4:-21560534 MS.gene026874:CDS
AGCTCTGCTGCTACCATTTC+CGG 0.255468 2.4:-21560660 MS.gene026874:CDS
TAAGTTAAGTACAGGAAATT+TGG 0.266307 2.4:-21560859 MS.gene026874:CDS
CGTTGCAAATCAACCGGTTT+CGG 0.305328 2.4:-21560592 MS.gene026874:CDS
ACAACATGGCAAAGTCTATA+AGG 0.357194 2.4:-21561300 MS.gene026874:CDS
CAATGCCTGCTCATCATTTG+TGG 0.376785 2.4:+21560623 None:intergenic
GGTGTAGCTGTTGTGGGTTA+TGG 0.384097 2.4:-21559452 MS.gene026874:intron
AATTATGCAGTTGGATAAGA+TGG 0.402123 2.4:+21559299 None:intergenic
AATATTTGTAGGATGTTGTT+GGG 0.406693 2.4:-21560916 MS.gene026874:intron
ATTGAATCTTTCAACAATGT+TGG 0.411583 2.4:-21561224 MS.gene026874:CDS
ATTGGTTGGTGAAGAATTCA+TGG 0.413527 2.4:-21559406 MS.gene026874:CDS
AGGTTGTGAAGGTGGTCTTA+TGG 0.416045 2.4:-21560784 MS.gene026874:CDS
TACCCTTATCAAGGTGTTGA+TGG 0.417342 2.4:-21560708 MS.gene026874:CDS
GCATCAATGGCTACCGAAAC+CGG 0.418768 2.4:+21560579 None:intergenic
TAATATTTGTAGGATGTTGT+TGG 0.420839 2.4:-21560917 MS.gene026874:intron
GACATTCTCGTACCCGGAAA+TGG 0.421059 2.4:+21560647 None:intergenic
CCCCTGGGTGTAGCTGTTGT+GGG 0.432312 2.4:-21559458 MS.gene026874:intron
GAATTCATGGGGAACTCAAT+GGG 0.434741 2.4:-21559393 MS.gene026874:CDS
GGGGTGAAGAAGGGTACATT+AGG 0.434781 2.4:-21559373 MS.gene026874:CDS
GCTCTGCTGCTACCATTTCC+GGG 0.441657 2.4:-21560659 MS.gene026874:CDS
TTCCATCAACACCTTGATAA+GGG 0.450919 2.4:+21560706 None:intergenic
GCTGTTGTGGGTTATGGTGT+TGG 0.453735 2.4:-21559446 MS.gene026874:CDS
AATGCCTGCTCATCATTTGT+GGG 0.454729 2.4:+21560624 None:intergenic
AAAAGCCGTTGCAAATCAAC+CGG 0.459968 2.4:-21560598 MS.gene026874:CDS
GTCAGAGCCACTAGCATCAA+TGG 0.460761 2.4:+21560566 None:intergenic
GGAACTCAATGGGGTGAAGA+AGG 0.462980 2.4:-21559383 MS.gene026874:CDS
GTTCCATCAACACCTTGATA+AGG 0.464383 2.4:+21560705 None:intergenic
TGCTCATGCCTCTCATACAT+AGG 0.466056 2.4:+21561332 None:intergenic
AAATCTTATAATTATGCAGT+TGG 0.468251 2.4:+21559290 None:intergenic
GCAGCTAGAAACAAATTCAA+TGG 0.472406 2.4:-21561140 MS.gene026874:CDS
ACTTAACTTATGAATTCCTT+CGG 0.480238 2.4:+21560872 None:intergenic
GCCCCTGGGTGTAGCTGTTG+TGG 0.481622 2.4:-21559459 MS.gene026874:intron
CATTAGGATGCAAAGGGGTG+TGG 0.492848 2.4:-21559357 MS.gene026874:CDS
AGAATTCATGGGGAACTCAA+TGG 0.494609 2.4:-21559394 MS.gene026874:CDS
TACCATCTGCAGTGGATTGG+AGG 0.508009 2.4:-21561064 MS.gene026874:CDS
TTCTAGCTGCTATAAACTCA+TGG 0.510437 2.4:+21561153 None:intergenic
GGAATTCATAAGTTAAGTAC+AGG 0.513328 2.4:-21560867 MS.gene026874:CDS
ACATGCAATAAAACTGAAGT+AGG 0.516912 2.4:-21560684 MS.gene026874:CDS
ATCTGCAGTGGATTGGAGGC+AGG 0.519791 2.4:-21561060 MS.gene026874:CDS
TGTCCCCACAAATGATGAGC+AGG 0.522234 2.4:-21560628 MS.gene026874:CDS
AGGGTACATTAGGATGCAAA+GGG 0.524704 2.4:-21559363 MS.gene026874:CDS
TGTGGGGACATTCTCGTACC+CGG 0.543427 2.4:+21560641 None:intergenic
AAATTCAATGGATATTTGCA+TGG 0.546885 2.4:-21561128 MS.gene026874:CDS
AAGGGTACATTAGGATGCAA+AGG 0.551462 2.4:-21559364 MS.gene026874:CDS
TAATGGACTTAGCACTGAAG+TGG 0.556033 2.4:-21560733 MS.gene026874:CDS
TTGGTTGGTGAAGAATTCAT+GGG 0.557529 2.4:-21559405 MS.gene026874:CDS
CGAAACCGGTTGATTTGCAA+CGG 0.558104 2.4:+21560593 None:intergenic
TCGGCTGTTGCATCAACCGA+AGG 0.561296 2.4:-21560888 MS.gene026874:CDS
TGTGGAACTGAGTTGGATCA+TGG 0.564256 2.4:-21560507 MS.gene026874:intron
GAACTCAATGGGGTGAAGAA+GGG 0.565034 2.4:-21559382 MS.gene026874:CDS
GAAGTGGAATACCCTTATCA+AGG 0.568429 2.4:-21560717 MS.gene026874:CDS
GTGAAGATGAGACTGAGTAT+TGG 0.569553 2.4:-21559424 MS.gene026874:CDS
ATGTACCATCTGCAGTGGAT+TGG 0.570020 2.4:-21561067 MS.gene026874:CDS
TCGGTAGCCATTGATGCTAG+TGG 0.574292 2.4:-21560573 MS.gene026874:CDS
TGCCTCCAATCCACTGCAGA+TGG 0.579586 2.4:+21561062 None:intergenic
TAAGACCACCTTCACAACCT+TGG 0.583050 2.4:+21560787 None:intergenic
TCTATAAGGATGCACATGAA+AGG 0.584380 2.4:-21561286 MS.gene026874:CDS
TGGTTCATGTGGAACTGAGT+TGG 0.588339 2.4:-21560514 MS.gene026874:CDS
AGGGGTGTGGATGCTTCAGA+AGG 0.605030 2.4:-21559344 MS.gene026874:CDS
TGTATGAGAGGCATGAGCAA+TGG 0.614507 2.4:-21561328 MS.gene026874:CDS
GCAGTGGATTGGAGGCAGGA+AGG 0.619784 2.4:-21561056 MS.gene026874:CDS
AGATGAGACTGAGTATTGGT+TGG 0.624949 2.4:-21559420 MS.gene026874:CDS
AAAATGACCCTATGTATGAG+AGG 0.630864 2.4:-21561340 None:intergenic
GAAGACCAAGGTTGTGAAGG+TGG 0.632713 2.4:-21560792 MS.gene026874:CDS
GGTGTCTTTACTGGTTCATG+TGG 0.637602 2.4:-21560525 MS.gene026874:CDS
GGTGAAGACCAAGGTTGTGA+AGG 0.642150 2.4:-21560795 MS.gene026874:CDS
GATGCTTCAGAAGGAATATG+TGG 0.650231 2.4:-21559335 MS.gene026874:CDS
CTTGTTGATTGTGACACAAG+TGG 0.664457 2.4:-21560816 MS.gene026874:CDS
AATTCATGGGGAACTCAATG+GGG 0.670782 2.4:-21559392 MS.gene026874:CDS
TAGTGATGTACCATCTGCAG+TGG 0.678103 2.4:-21561072 MS.gene026874:CDS
TGAGAGGCATGAGCAATGGA+TGG 0.686001 2.4:-21561324 MS.gene026874:CDS
ATGCCTGCTCATCATTTGTG+GGG 0.688383 2.4:+21560625 None:intergenic
TGGTTGGTGAAGAATTCATG+GGG 0.692894 2.4:-21559404 MS.gene026874:CDS
GAGCAATGGATGGTACAACA+TGG 0.707448 2.4:-21561314 MS.gene026874:CDS
GACACAAGTGGTGAAGACCA+AGG 0.761833 2.4:-21560804 MS.gene026874:CDS
GGGTACATTAGGATGCAAAG+GGG 0.770853 2.4:-21559362 MS.gene026874:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAAAAAAAAAAAAAGG+GGG + chr2.4:21560224-21560243 None:intergenic 10.0%
!! GAATTCATTATAAAAAATAA+TGG - chr2.4:21559880-21559899 MS.gene026874:intron 10.0%
!!! TTAAGTTTTTAAAATTGTTA+GGG - chr2.4:21560618-21560637 MS.gene026874:CDS 10.0%
!!! TTTAAGTTTTTAAAATTGTT+AGG - chr2.4:21560617-21560636 MS.gene026874:CDS 10.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr2.4:21560202-21560221 None:intergenic 100.0%
!! AAAAACTTAAAACAATCAAT+TGG + chr2.4:21560608-21560627 None:intergenic 15.0%
!! AATTTCTAATTAATAAGTTG+TGG - chr2.4:21560768-21560787 MS.gene026874:CDS 15.0%
!! GGAAATAAATCTTATAAATT+AGG - chr2.4:21559427-21559446 MS.gene026874:CDS 15.0%
!! TCAACAATTAATGATATATA+TGG - chr2.4:21560292-21560311 MS.gene026874:intron 15.0%
!! TTTAAAAAAAAAAAAAAGGG+GGG + chr2.4:21560223-21560242 None:intergenic 15.0%
!!! AACTAAATTTTACTATTACA+AGG + chr2.4:21559687-21559706 None:intergenic 15.0%
!! AATTCGACTAATTAATTGTT+AGG - chr2.4:21560697-21560716 MS.gene026874:CDS 20.0%
!! AGTAACCTATGTAATAAAAT+GGG - chr2.4:21560918-21560937 MS.gene026874:intron 20.0%
!! AGTTGTGATTTAATATTTGT+AGG - chr2.4:21559703-21559722 MS.gene026874:intron 20.0%
!! ATTCGACTAATTAATTGTTA+GGG - chr2.4:21560698-21560717 MS.gene026874:CDS 20.0%
!! TTAAAAAAAAAAAAAAGGGG+GGG + chr2.4:21560222-21560241 None:intergenic 20.0%
!! TTTATATAACAATCAAGATG+TGG - chr2.4:21559647-21559666 MS.gene026874:intron 20.0%
!! TTTCTATATAAGTTACAAGA+CGG + chr2.4:21560962-21560981 None:intergenic 20.0%
!!! GTTTTATATTGATTTTGAGT+AGG - chr2.4:21560674-21560693 MS.gene026874:CDS 20.0%
! AAATTCAATGGATATTTGCA+TGG - chr2.4:21559502-21559521 MS.gene026874:intron 25.0%
! AATATTTGTAGGATGTTGTT+GGG - chr2.4:21559714-21559733 MS.gene026874:intron 25.0%
! ACTTAACTTATGAATTCCTT+CGG + chr2.4:21559761-21559780 None:intergenic 25.0%
! ATTGAATCTTTCAACAATGT+TGG - chr2.4:21559406-21559425 MS.gene026874:CDS 25.0%
! ATTGCAATTTAAGAGAGAAA+GGG + chr2.4:21560486-21560505 None:intergenic 25.0%
! CTTCAAAAGTTTATAAACCA+TGG + chr2.4:21560829-21560848 None:intergenic 25.0%
! GAGTAACCTATGTAATAAAA+TGG - chr2.4:21560917-21560936 MS.gene026874:intron 25.0%
! TAAAAAAAAAAAAAAGGGGG+GGG + chr2.4:21560221-21560240 None:intergenic 25.0%
! TAAGCAAAAGTTTACACTTT+AGG - chr2.4:21560264-21560283 MS.gene026874:intron 25.0%
! TAAGTTAAGTACAGGAAATT+TGG - chr2.4:21559771-21559790 MS.gene026874:intron 25.0%
! TAATATTTGTAGGATGTTGT+TGG - chr2.4:21559713-21559732 MS.gene026874:intron 25.0%
!! AAATTTGAAATTGAGAGAGA+TGG + chr2.4:21560528-21560547 None:intergenic 25.0%
!! ATATATATGGTCTTTGTTGT+AGG - chr2.4:21560305-21560324 MS.gene026874:intron 25.0%
!! GACTTTCAATTTTACAAAAG+TGG - chr2.4:21560084-21560103 MS.gene026874:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG - chr2.4:21560366-21560385 MS.gene026874:intron 25.0%
!!! GGTTTATAAACTTTTGAAGT+TGG - chr2.4:21560830-21560849 MS.gene026874:CDS 25.0%
!!! TTTAGCTCATTTTCAACAAA+TGG - chr2.4:21561016-21561035 MS.gene026874:intron 25.0%
!!! TTTTTTTTAAATACACCCTC+CGG - chr2.4:21560232-21560251 MS.gene026874:intron 25.0%
AAAAAAAAAAAAAAGGGGGG+GGG + chr2.4:21560220-21560239 None:intergenic 30.0%
AAACAATCAATTGGAGAATG+AGG + chr2.4:21560599-21560618 None:intergenic 30.0%
AACAATCAATTGGAGAATGA+GGG + chr2.4:21560598-21560617 None:intergenic 30.0%
AATAAGTTGTGGAAAACTTG+TGG - chr2.4:21560779-21560798 MS.gene026874:CDS 30.0%
ACATGCAATAAAACTGAAGT+AGG - chr2.4:21559946-21559965 MS.gene026874:intron 30.0%
ATCCAGACTTTAATTATACG+TGG + chr2.4:21561149-21561168 None:intergenic 30.0%
GATTGCAATTTAAGAGAGAA+AGG + chr2.4:21560487-21560506 None:intergenic 30.0%
GGAATTCATAAGTTAAGTAC+AGG - chr2.4:21559763-21559782 MS.gene026874:intron 30.0%
GTAAATGATTGATGTCACTA+AGG + chr2.4:21560647-21560666 None:intergenic 30.0%
GTAATAAAATGGGTGATGAA+TGG - chr2.4:21560928-21560947 MS.gene026874:intron 30.0%
TTGCAATTTAAGAGAGAAAG+GGG + chr2.4:21560485-21560504 None:intergenic 30.0%
! CATCACCCATTTTATTACAT+AGG + chr2.4:21560926-21560945 None:intergenic 30.0%
! CTCATTTTCAACAAATGGAT+AGG - chr2.4:21561021-21561040 MS.gene026874:CDS 30.0%
! CTTTTGCTTATAATAGTGAC+CGG + chr2.4:21560254-21560273 None:intergenic 30.0%
!! CTTTTGTTTAATATACCCTC+CGG + chr2.4:21560388-21560407 None:intergenic 30.0%
AAAAAAAAAAAAAGGGGGGG+GGG + chr2.4:21560219-21560238 None:intergenic 35.0%
ACAACATGGCAAAGTCTATA+AGG - chr2.4:21559330-21559349 MS.gene026874:CDS 35.0%
ACAATCAATTGGAGAATGAG+GGG + chr2.4:21560597-21560616 None:intergenic 35.0%
CAACACGAATTCATTTCGAA+AGG + chr2.4:21560737-21560756 None:intergenic 35.0%
CTCCACGTATAATTAAAGTC+TGG - chr2.4:21561144-21561163 MS.gene026874:CDS 35.0%
GCAGCTAGAAACAAATTCAA+TGG - chr2.4:21559490-21559509 MS.gene026874:intron 35.0%
GTGAAAAATCAAGGTCAATG+TGG - chr2.4:21559610-21559629 MS.gene026874:intron 35.0%
TACAAAAGTGGTGTCTTTAC+TGG - chr2.4:21560096-21560115 MS.gene026874:intron 35.0%
TCTATAAGGATGCACATGAA+AGG - chr2.4:21559344-21559363 MS.gene026874:CDS 35.0%
TTCAACAAATGGATAGGAGA+CGG - chr2.4:21561027-21561046 MS.gene026874:CDS 35.0%
TTCCATCAACACCTTGATAA+GGG + chr2.4:21559927-21559946 None:intergenic 35.0%
! CATGAAAGGCAAAAACGTTT+TGG - chr2.4:21559358-21559377 MS.gene026874:CDS 35.0%
! CATTGACCTTGATTTTTCAC+AGG + chr2.4:21559610-21559629 None:intergenic 35.0%
! TTCTAGCTGCTATAAACTCA+TGG + chr2.4:21559480-21559499 None:intergenic 35.0%
!! ATTGGTTGGTGAAGAATTCA+TGG - chr2.4:21561224-21561243 MS.gene026874:CDS 35.0%
!! TTGGTTGGTGAAGAATTCAT+GGG - chr2.4:21561225-21561244 MS.gene026874:CDS 35.0%
AAAAAAAAAAAAGGGGGGGG+GGG + chr2.4:21560218-21560237 None:intergenic 40.0%
AAAAGCCGTTGCAAATCAAC+CGG - chr2.4:21560032-21560051 MS.gene026874:intron 40.0%
AATGCCTGCTCATCATTTGT+GGG + chr2.4:21560009-21560028 None:intergenic 40.0%
AATTCATGGGGAACTCAATG+GGG - chr2.4:21561238-21561257 MS.gene026874:CDS 40.0%
AGAATTCATGGGGAACTCAA+TGG - chr2.4:21561236-21561255 MS.gene026874:CDS 40.0%
AGGGTACATTAGGATGCAAA+GGG - chr2.4:21561267-21561286 MS.gene026874:CDS 40.0%
ATTGCTTACCTTAGAGTGAG+AGG + chr2.4:21560571-21560590 None:intergenic 40.0%
CAATCAATTGGAGAATGAGG+GGG + chr2.4:21560596-21560615 None:intergenic 40.0%
GAAGTGGAATACCCTTATCA+AGG - chr2.4:21559913-21559932 MS.gene026874:intron 40.0%
GAATTCATGGGGAACTCAAT+GGG - chr2.4:21561237-21561256 MS.gene026874:CDS 40.0%
GATGCTTCAGAAGGAATATG+TGG - chr2.4:21561295-21561314 MS.gene026874:CDS 40.0%
GTCACACCTGTGAAAAATCA+AGG - chr2.4:21559601-21559620 MS.gene026874:intron 40.0%
GTGAAGATGAGACTGAGTAT+TGG - chr2.4:21561206-21561225 MS.gene026874:CDS 40.0%
GTTCCATCAACACCTTGATA+AGG + chr2.4:21559928-21559947 None:intergenic 40.0%
TAAGTGCAACACATTTGCCA+TGG - chr2.4:21560809-21560828 MS.gene026874:CDS 40.0%
TGCTTATAATAGTGACCGGA+GGG - chr2.4:21560370-21560389 MS.gene026874:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG + chr2.4:21560389-21560370 None:intergenic 40.0%
TGTTTAGTTACGGTACAGGA+AGG + chr2.4:21560460-21560479 None:intergenic 40.0%
TTACGGTACAGGAAGGAAAT+TGG + chr2.4:21560453-21560472 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr2.4:21560369-21560388 MS.gene026874:intron 40.0%
TTGCTTATAATAGTGACCGG+AGG + chr2.4:21560388-21560369 None:intergenic 40.0%
! AAGGGTACATTAGGATGCAA+AGG - chr2.4:21561266-21561285 MS.gene026874:CDS 40.0%
! CTTGTTGATTGTGACACAAG+TGG - chr2.4:21559814-21559833 MS.gene026874:intron 40.0%
!! AGATGAGACTGAGTATTGGT+TGG - chr2.4:21561210-21561229 MS.gene026874:CDS 40.0%
!! TAATGGACTTAGCACTGAAG+TGG - chr2.4:21559897-21559916 MS.gene026874:intron 40.0%
!! TACCCTTATCAAGGTGTTGA+TGG - chr2.4:21559922-21559941 MS.gene026874:intron 40.0%
!! TGGTTGGTGAAGAATTCATG+GGG - chr2.4:21561226-21561245 MS.gene026874:CDS 40.0%
AAAAAAAAAAAGGGGGGGGG+GGG + chr2.4:21560217-21560236 None:intergenic 45.0%
AACTCTCACCTCTCACTCTA+AGG - chr2.4:21560560-21560579 MS.gene026874:CDS 45.0%
AGAGGTGAGAGTTGAGAAGT+AGG + chr2.4:21560553-21560572 None:intergenic 45.0%
ATGCCTGCTCATCATTTGTG+GGG + chr2.4:21560008-21560027 None:intergenic 45.0%
ATGTACCATCTGCAGTGGAT+TGG - chr2.4:21559563-21559582 MS.gene026874:intron 45.0%
CAATGCCTGCTCATCATTTG+TGG + chr2.4:21560010-21560029 None:intergenic 45.0%
GAACTCAATGGGGTGAAGAA+GGG - chr2.4:21561248-21561267 MS.gene026874:CDS 45.0%
GAGCAATGGATGGTACAACA+TGG - chr2.4:21559316-21559335 MS.gene026874:CDS 45.0%
GATGCATGTAGGAGCAATGT+GGG - chr2.4:21560160-21560179 MS.gene026874:intron 45.0%
GGGTACATTAGGATGCAAAG+GGG - chr2.4:21561268-21561287 MS.gene026874:CDS 45.0%
TAAGACCACCTTCACAACCT+TGG + chr2.4:21559846-21559865 None:intergenic 45.0%
TAGTGATGTACCATCTGCAG+TGG - chr2.4:21559558-21559577 MS.gene026874:intron 45.0%
TGGTTCATGTGGAACTGAGT+TGG - chr2.4:21560116-21560135 MS.gene026874:intron 45.0%
! AGGATGTTGTTGGGCGTTTT+CGG - chr2.4:21559723-21559742 MS.gene026874:intron 45.0%
! AGGTTGTGAAGGTGGTCTTA+TGG - chr2.4:21559846-21559865 MS.gene026874:intron 45.0%
! CGTTGCAAATCAACCGGTTT+CGG - chr2.4:21560038-21560057 MS.gene026874:intron 45.0%
! GAAAGGGGCGTGTTTAGTTA+CGG + chr2.4:21560470-21560489 None:intergenic 45.0%
! TGTATGAGAGGCATGAGCAA+TGG - chr2.4:21559302-21559321 MS.gene026874:CDS 45.0%
! TGTGGAACTGAGTTGGATCA+TGG - chr2.4:21560123-21560142 MS.gene026874:intron 45.0%
!! CGAAACCGGTTGATTTGCAA+CGG + chr2.4:21560040-21560059 None:intergenic 45.0%
!! GGTGTCTTTACTGGTTCATG+TGG - chr2.4:21560105-21560124 MS.gene026874:intron 45.0%
!! GTATTTAAAAAAAAAAAAAA+GGG + chr2.4:21560226-21560245 None:intergenic 5.0%
!! TATTTAAAAAAAAAAAAAAG+GGG + chr2.4:21560225-21560244 None:intergenic 5.0%
!! TGTATTTAAAAAAAAAAAAA+AGG + chr2.4:21560227-21560246 None:intergenic 5.0%
AAAAAAAAAAGGGGGGGGGG+GGG + chr2.4:21560216-21560235 None:intergenic 50.0%
AGCTCTGCTGCTACCATTTC+CGG - chr2.4:21559970-21559989 MS.gene026874:intron 50.0%
AGTTGGATCATGGTGTAGCC+AGG - chr2.4:21560133-21560152 MS.gene026874:intron 50.0%
CATTAGGATGCAAAGGGGTG+TGG - chr2.4:21561273-21561292 MS.gene026874:CDS 50.0%
GAAGACCAAGGTTGTGAAGG+TGG - chr2.4:21559838-21559857 MS.gene026874:intron 50.0%
GACACAAGTGGTGAAGACCA+AGG - chr2.4:21559826-21559845 MS.gene026874:intron 50.0%
GACATTCTCGTACCCGGAAA+TGG + chr2.4:21559986-21560005 None:intergenic 50.0%
GCATCAATGGCTACCGAAAC+CGG + chr2.4:21560054-21560073 None:intergenic 50.0%
GGAACTCAATGGGGTGAAGA+AGG - chr2.4:21561247-21561266 MS.gene026874:CDS 50.0%
GGATGCATGTAGGAGCAATG+TGG - chr2.4:21560159-21560178 MS.gene026874:intron 50.0%
GGCGTGTTTAGTTACGGTAC+AGG + chr2.4:21560464-21560483 None:intergenic 50.0%
GGTGAAGACCAAGGTTGTGA+AGG - chr2.4:21559835-21559854 MS.gene026874:intron 50.0%
GTCAGAGCCACTAGCATCAA+TGG + chr2.4:21560067-21560086 None:intergenic 50.0%
GTTGGATCATGGTGTAGCCA+GGG - chr2.4:21560134-21560153 MS.gene026874:intron 50.0%
TCGGTAGCCATTGATGCTAG+TGG - chr2.4:21560057-21560076 MS.gene026874:intron 50.0%
TGTCCCCACAAATGATGAGC+AGG - chr2.4:21560002-21560021 MS.gene026874:intron 50.0%
TTGGATCATGGTGTAGCCAG+GGG - chr2.4:21560135-21560154 MS.gene026874:intron 50.0%
! GCTGTTGTGGGTTATGGTGT+TGG - chr2.4:21561184-21561203 MS.gene026874:CDS 50.0%
! GGGGTGAAGAAGGGTACATT+AGG - chr2.4:21561257-21561276 MS.gene026874:CDS 50.0%
! GGTGTAGCTGTTGTGGGTTA+TGG - chr2.4:21561178-21561197 MS.gene026874:CDS 50.0%
! TGAGAGGCATGAGCAATGGA+TGG - chr2.4:21559306-21559325 MS.gene026874:CDS 50.0%
!! GAAGTTGGCTTTGCTCCTGT+CGG - chr2.4:21560845-21560864 MS.gene026874:CDS 50.0%
!! TACCATCTGCAGTGGATTGG+AGG - chr2.4:21559566-21559585 MS.gene026874:intron 50.0%
AAAAAAAAAGGGGGGGGGGG+GGG + chr2.4:21560215-21560234 None:intergenic 55.0%
AACCCACAACAGCTACACCC+AGG + chr2.4:21561177-21561196 None:intergenic 55.0%
ACCCACAACAGCTACACCCA+GGG + chr2.4:21561176-21561195 None:intergenic 55.0%
CACTTGCAGTTGCTACCGAC+AGG + chr2.4:21560863-21560882 None:intergenic 55.0%
GCTCTGCTGCTACCATTTCC+GGG - chr2.4:21559971-21559990 MS.gene026874:intron 55.0%
TAAAGTCTGGATCCGCCCCT+GGG - chr2.4:21561157-21561176 MS.gene026874:CDS 55.0%
TCGGCTGTTGCATCAACCGA+AGG - chr2.4:21559742-21559761 MS.gene026874:intron 55.0%
TGCCTCCAATCCACTGCAGA+TGG + chr2.4:21559571-21559590 None:intergenic 55.0%
TGTGGGGACATTCTCGTACC+CGG + chr2.4:21559992-21560011 None:intergenic 55.0%
TTAAAGTCTGGATCCGCCCC+TGG - chr2.4:21561156-21561175 MS.gene026874:CDS 55.0%
! AGGGGTGTGGATGCTTCAGA+AGG - chr2.4:21561286-21561305 MS.gene026874:CDS 55.0%
!! ATCTGCAGTGGATTGGAGGC+AGG - chr2.4:21559570-21559589 MS.gene026874:intron 55.0%
AAAAAAAAGGGGGGGGGGGG+GGG + chr2.4:21560214-21560233 None:intergenic 60.0%
ACAACAGCTACACCCAGGGG+CGG + chr2.4:21561172-21561191 None:intergenic 60.0%
CCCACAACAGCTACACCCAG+GGG + chr2.4:21561175-21561194 None:intergenic 60.0%
CCCCTGGGTGTAGCTGTTGT+GGG - chr2.4:21561172-21561191 MS.gene026874:CDS 60.0%
GATCATGGTGTAGCCAGGGG+CGG - chr2.4:21560138-21560157 MS.gene026874:intron 60.0%
!! GCAGTGGATTGGAGGCAGGA+AGG - chr2.4:21559574-21559593 MS.gene026874:intron 60.0%
AAAAAAAGGGGGGGGGGGGG+GGG + chr2.4:21560213-21560232 None:intergenic 65.0%
AGCCAGGGGCGGATGCATGT+AGG - chr2.4:21560149-21560168 MS.gene026874:intron 65.0%
CTCCTACATGCATCCGCCCC+TGG + chr2.4:21560154-21560173 None:intergenic 65.0%
GCCCCTGGGTGTAGCTGTTG+TGG - chr2.4:21561171-21561190 MS.gene026874:CDS 65.0%
AAAAAAGGGGGGGGGGGGGG+GGG + chr2.4:21560212-21560231 None:intergenic 70.0%
AAAAAGGGGGGGGGGGGGGG+GGG + chr2.4:21560211-21560230 None:intergenic 75.0%
! AAAAGGGGGGGGGGGGGGGG+GGG + chr2.4:21560210-21560229 None:intergenic 80.0%
!! GGGGGGGGGGGGGAAGCATG+TGG + chr2.4:21560195-21560214 None:intergenic 80.0%
! AAAGGGGGGGGGGGGGGGGG+GGG + chr2.4:21560209-21560228 None:intergenic 85.0%
! AAGGGGGGGGGGGGGGGGGG+GGG + chr2.4:21560208-21560227 None:intergenic 90.0%
! AGGGGGGGGGGGGGGGGGGG+GGG + chr2.4:21560207-21560226 None:intergenic 95.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 21559301 21561351 21559301 ID=MS.gene026874
chr2.4 mRNA 21559301 21561351 21559301 ID=MS.gene026874.t1;Parent=MS.gene026874
chr2.4 exon 21561021 21561351 21561021 ID=MS.gene026874.t1.exon1;Parent=MS.gene026874.t1
chr2.4 CDS 21561021 21561351 21561021 ID=cds.MS.gene026874.t1;Parent=MS.gene026874.t1
chr2.4 exon 21560508 21560927 21560508 ID=MS.gene026874.t1.exon2;Parent=MS.gene026874.t1
chr2.4 CDS 21560508 21560927 21560508 ID=cds.MS.gene026874.t1;Parent=MS.gene026874.t1
chr2.4 exon 21559301 21559467 21559301 ID=MS.gene026874.t1.exon3;Parent=MS.gene026874.t1
chr2.4 CDS 21559301 21559467 21559301 ID=cds.MS.gene026874.t1;Parent=MS.gene026874.t1
Gene Sequence

>MS.gene026874

ATGTATGAGAGGCATGAGCAATGGATGGTACAACATGGCAAAGTCTATAAGGATGCACATGAAAGGCAAAAACGTTTTGGAATATTCAAAGAAAATGTAAATTACATTGAATCTTTCAACAATGTTGGAAATAAATCTTATAAATTAGGACTTAATCATTTTGCTGATCTTAGCAACCATGAGTTTATAGCAGCTAGAAACAAATTCAATGGATATTTGCATGGCTCAAGCATAACTACTTTTAAGTACAAAAATGTTAGTGATGTACCATCTGCAGTGGATTGGAGGCAGGAAGGAGCAGTCACACCTGTGAAAAATCAAGGTCAATGTGGTAAGTGTTTATTTATTTATATAACAATCAAGATGTGGTTTCTTATTTTTAACCTTGTAATAGTAAAATTTAGTTGTGATTTAATATTTGTAGGATGTTGTTGGGCGTTTTCGGCTGTTGCATCAACCGAAGGAATTCATAAGTTAAGTACAGGAAATTTGGTCTCTTTATCAGAACAAGAACTTGTTGATTGTGACACAAGTGGTGAAGACCAAGGTTGTGAAGGTGGTCTTATGGATGATGCTTTCGAATTCATTATAAAAAATAATGGACTTAGCACTGAAGTGGAATACCCTTATCAAGGTGTTGATGGAACATGCAATAAAACTGAAGTAGGCAGCTCTGCTGCTACCATTTCCGGGTACGAGAATGTCCCCACAAATGATGAGCAGGCATTGCAAAAAGCCGTTGCAAATCAACCGGTTTCGGTAGCCATTGATGCTAGTGGCTCTGACTTTCAATTTTACAAAAGTGGTGTCTTTACTGGTTCATGTGGAACTGAGTTGGATCATGGTGTAGCCAGGGGCGGATGCATGTAGGAGCAATGTGGGACTATAGCTCCACATGCTTCCCCCCCCCCCCCCCCCCCCCCCCTTTTTTTTTTTTTTAAATACACCCTCCGGTCACTATTATAAGCAAAAGTTTACACTTTAGGTTCATTCAACAATTAATGATATATATGGTCTTTGTTGTAGGTCATATACATCATTCAATGAATGAATCTAAAATATAAATTTTTGCTTATAATAGTGACCGGAGGGTATATTAAACAAAAGACAACTGTGTGTCTCACTCTCACTCTCTTTTCATTTTCTATTCCAATTTCCTTCCTGTACCGTAACTAAACACGCCCCTTTCTCTCTTAAATTGCAATCTCTCTCAATCTCAGCTCTCCATCTCTCTCAATTTCAAATTTCTCCTACTTCTCAACTCTCACCTCTCACTCTAAGGTAAGCAATTTCCCCCTCATTCTCCAATTGATTGTTTTAAGTTTTTAAAATTGTTAGGGTTTCCTTAGTGACATCAATCATTTACTAAGAATGTTTTATATTGATTTTGAGTAGGAATTCGACTAATTAATTGTTAGGGATTCATGTTATTACCTTTCGAAATGAATTCGTGTTGTATGCAATGTTAATTTCTAATTAATAAGTTGTGGAAAACTTGTGGAAACAAATAAGTGCAACACATTTGCCATGGTTTATAAACTTTTGAAGTTGGCTTTGCTCCTGTCGGTAGCAACTGCAAGTGTTGAACGTGTTTTTTCAGCTATGAAAGTTGTGAAGAGTAACCTATGTAATAAAATGGGTGATGAATGGCTAAATGACCGTCTTGTAACTTATATAGAAAGAGATGTTCTTTTAACAATTAACAATGATGTTATTTTAGCTCATTTTCAACAAATGGATAGGAGACGGTTTTCTTTGTAATGTATTGCACTCTATAACAGAAAGAGATGTTAATTCTTATTTTTATTATAGTTTGCATTATTGTTTTTTTTAGAAATTTTAGCTCCACGTATAATTAAAGTCTGGATCCGCCCCTGGGTGTAGCTGTTGTGGGTTATGGTGTTGGTGAAGATGAGACTGAGTATTGGTTGGTGAAGAATTCATGGGGAACTCAATGGGGTGAAGAAGGGTACATTAGGATGCAAAGGGGTGTGGATGCTTCAGAAGGAATATGTGGTATTGCAATGCATCCATCTTATCCAACTGCATAA
Protein sequence

>MS.gene026874.t1

MYERHEQWMVQHGKVYKDAHERQKRFGIFKENVNYIESFNNVGNKSYKLGLNHFADLSNHEFIAARNKFNGYLHGSSITTFKYKNVSDVPSAVDWRQEGAVTPVKNQGQCGCCWAFSAVASTEGIHKLSTGNLVSLSEQELVDCDTSGEDQGCEGGLMDDAFEFIIKNNGLSTEVEYPYQGVDGTCNKTEVGSSAATISGYENVPTNDEQALQKAVANQPVSVAIDASGSDFQFYKSGVFTGSCGTELDHAVVGYGVGEDETEYWLVKNSWGTQWGEEGYIRMQRGVDASEGICGIAMHPSYPTA