AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027420.t1 MTR_4g047610 96.221 344 12 1 1 344 1 343 0.0 671
MS.gene027420.t1 MTR_5g022560 89.535 344 36 0 1 344 1 344 0.0 655
MS.gene027420.t1 MTR_4g079770 88.150 346 38 3 1 344 1 345 0.0 634
MS.gene027420.t1 MTR_4g107930 86.377 345 46 1 1 344 1 345 0.0 628
MS.gene027420.t1 MTR_4g079470 88.615 325 35 2 21 344 22 345 0.0 603
MS.gene027420.t1 MTR_4g079800 85.538 325 45 2 21 344 22 345 0.0 585
MS.gene027420.t1 MTR_2g075830 74.709 344 84 2 1 344 1 341 0.0 530
MS.gene027420.t1 MTR_4g080700 64.198 324 104 6 25 344 25 340 1.03e-151 430
MS.gene027420.t1 MTR_4g080730 61.782 348 121 6 1 344 1 340 1.83e-151 429
MS.gene027420.t1 MTR_4g080360 52.464 345 157 5 2 344 3 342 2.56e-131 379
MS.gene027420.t1 MTR_2g083930 53.846 338 141 5 12 342 13 342 6.50e-131 377
MS.gene027420.t1 MTR_4g104370 55.102 343 141 9 7 342 6 342 3.44e-127 369
MS.gene027420.t1 MTR_3g075390 53.079 341 144 7 14 342 7 343 1.69e-124 362
MS.gene027420.t1 MTR_4g079440 55.161 310 133 3 33 342 40 343 1.08e-121 354
MS.gene027420.t1 MTR_2g090890 50.000 340 156 6 10 343 5 336 7.46e-118 344
MS.gene027420.t1 MTR_7g078600 56.452 310 124 6 34 342 37 336 8.44e-118 344
MS.gene027420.t1 MTR_3g116080 52.924 342 144 9 11 343 14 347 3.11e-117 343
MS.gene027420.t1 MTR_7g079230 50.877 342 160 5 1 342 1 334 5.98e-116 339
MS.gene027420.t1 MTR_1g107550 52.681 317 139 6 32 342 43 354 7.55e-114 338
MS.gene027420.t1 MTR_4g094918 46.939 343 166 6 10 343 6 341 2.20e-112 331
MS.gene027420.t1 MTR_3g102220 49.541 327 147 7 27 342 41 360 4.33e-108 324
MS.gene027420.t1 MTR_1g018840 48.295 352 153 8 8 342 21 360 7.84e-108 323
MS.gene027420.t1 MTR_1g018840 48.295 352 153 8 8 342 21 360 9.16e-108 323
MS.gene027420.t1 MTR_3g112430 49.839 311 143 5 40 343 37 341 5.41e-101 305
MS.gene027420.t1 MTR_2g015850 53.103 290 129 5 55 342 78 362 2.72e-96 290
MS.gene027420.t1 MTR_2g016180 44.638 345 172 9 11 343 18 355 1.52e-90 280
MS.gene027420.t1 MTR_2g016270 44.737 342 161 9 11 343 18 340 1.26e-88 274
MS.gene027420.t1 MTR_2g067900 46.688 317 148 12 38 341 15 323 4.75e-80 247
MS.gene027420.t1 MTR_2g064475 41.714 350 147 16 38 343 15 351 3.51e-75 236
MS.gene027420.t1 MTR_2g067560 45.483 321 144 14 38 338 15 324 5.45e-74 245
MS.gene027420.t1 MTR_2g066980 36.592 358 166 13 7 343 1 318 2.46e-70 222
MS.gene027420.t1 MTR_7g078630 47.038 287 110 7 28 303 695 950 3.17e-70 236
MS.gene027420.t1 MTR_4g065077 38.889 324 175 9 32 343 44 356 5.22e-70 223
MS.gene027420.t1 MTR_2g015920 40.498 321 172 10 33 343 47 358 8.20e-70 223
MS.gene027420.t1 MTR_4g125300 41.424 309 162 10 41 343 53 348 2.77e-68 218
MS.gene027420.t1 MTR_4g071060 37.293 362 186 13 9 340 3 353 4.45e-65 210
MS.gene027420.t1 MTR_2g015930 36.813 364 201 12 1 343 3 358 5.09e-65 210
MS.gene027420.t1 MTR_8g086470 37.267 322 167 9 32 343 60 356 2.35e-63 206
MS.gene027420.t1 MTR_1g023210 36.982 338 170 12 23 335 33 352 1.15e-62 204
MS.gene027420.t1 MTR_1g023810 46.256 227 111 7 120 341 41 261 2.01e-59 192
MS.gene027420.t1 MTR_1g094135 35.294 357 196 11 7 340 19 363 4.00e-58 192
MS.gene027420.t1 MTR_4g072790 33.993 303 158 10 39 335 46 312 1.52e-42 150
MS.gene027420.t1 MTR_4g072340 33.333 303 158 9 41 335 50 316 5.41e-42 149
MS.gene027420.t1 MTR_4g073660 32.673 303 160 9 41 335 49 315 1.32e-41 148
MS.gene027420.t1 MTR_2g015840 46.497 157 81 2 7 161 1 156 9.72e-41 144
MS.gene027420.t1 MTR_2g015840 55.357 112 49 1 232 343 161 271 3.28e-35 129
MS.gene027420.t1 MTR_7g078580 62.245 98 36 1 245 342 4 100 3.38e-39 135
MS.gene027420.t1 MTR_7g079130 66.667 90 30 0 253 342 7 96 3.34e-38 132
MS.gene027420.t1 MTR_1g025450 41.143 175 78 7 126 296 55 208 3.77e-32 126
MS.gene027420.t1 MTR_2g016380 47.500 120 58 3 227 343 18 135 5.93e-28 109
MS.gene027420.t1 MTR_7g078310 44.286 140 69 3 82 219 115 247 1.04e-27 109
MS.gene027420.t1 MTR_7g111060 28.112 249 147 8 112 335 89 330 4.51e-23 98.6
MS.gene027420.t1 MTR_3g029560 28.125 256 140 9 98 343 16 237 1.99e-22 94.7
MS.gene027420.t1 MTR_7g111050 27.586 261 155 9 110 343 84 337 2.38e-21 94.0
MS.gene027420.t1 MTR_7g079260 56.944 72 15 1 269 340 7 62 6.71e-21 86.7
MS.gene027420.t1 MTR_8g087510 40.458 131 71 5 167 295 23 148 3.66e-19 84.0
MS.gene027420.t1 MTR_2g047960 48.235 85 43 1 135 219 7 90 1.23e-17 77.8
MS.gene027420.t1 MTR_3g082370 26.471 204 130 5 131 319 5 203 3.47e-17 80.1
MS.gene027420.t1 MTR_7g111050 25.339 221 137 7 110 305 79 296 1.45e-11 64.7
MS.gene027420.t1 MTR_1g042480 35.106 94 58 2 9 101 7 98 2.68e-11 60.8
MS.gene027420.t1 MTR_1g042360 35.106 94 58 2 9 101 7 98 2.68e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027420.t1 AT5G45890 55.942 345 142 6 7 343 3 345 1.54e-135 389
MS.gene027420.t1 AT5G50260 53.666 341 142 6 14 342 5 341 4.64e-127 369
MS.gene027420.t1 AT3G48340 54.651 344 138 8 12 342 4 342 1.58e-123 359
MS.gene027420.t1 AT4G35350 50.578 346 155 7 7 343 14 352 4.78e-117 343
MS.gene027420.t1 AT3G49340 49.415 342 148 7 20 344 8 341 1.81e-116 341
MS.gene027420.t1 AT2G34080 47.953 342 167 5 10 344 8 345 3.34e-116 340
MS.gene027420.t1 AT3G19390 50.151 331 161 4 14 343 18 345 1.70e-115 342
MS.gene027420.t1 AT2G27420 48.711 349 160 7 10 344 5 348 7.13e-114 334
MS.gene027420.t1 AT3G48350 54.259 317 136 7 32 343 31 343 1.17e-113 334
MS.gene027420.t1 AT5G43060 52.147 326 142 7 24 342 34 352 1.03e-112 335
MS.gene027420.t1 AT1G06260 50.146 343 152 7 9 342 9 341 4.52e-110 325
MS.gene027420.t1 AT1G20850 51.118 313 147 4 33 343 45 353 5.70e-110 325
MS.gene027420.t1 AT4G36880 50.154 325 144 6 32 343 42 361 8.68e-110 325
MS.gene027420.t1 AT3G19400 50.163 307 149 4 39 343 44 348 6.11e-109 322
MS.gene027420.t1 AT1G29090 46.797 359 163 9 4 344 7 355 8.29e-109 322
MS.gene027420.t1 AT1G47128 50.809 309 140 7 39 342 50 351 6.97e-108 323
MS.gene027420.t1 AT1G29080 45.509 334 160 7 24 344 22 346 5.95e-102 304
MS.gene027420.t1 AT1G09850 49.355 310 154 3 33 342 26 332 2.55e-101 305
MS.gene027420.t1 AT4G23520 45.143 350 167 9 10 342 7 348 1.42e-100 301
MS.gene027420.t1 AT4G11310 46.278 309 153 6 40 342 50 351 3.36e-97 292
MS.gene027420.t1 AT4G11320 44.762 315 149 6 40 342 57 358 6.10e-96 290
MS.gene027420.t1 AT4G11320 44.762 315 149 6 40 342 57 358 6.10e-96 290
MS.gene027420.t1 AT3G43960 43.202 331 181 6 16 342 18 345 1.28e-87 268
MS.gene027420.t1 AT1G29110 41.925 322 164 7 29 343 28 333 7.60e-85 260
MS.gene027420.t1 AT4G35350 48.214 280 129 7 7 277 14 286 2.07e-84 257
MS.gene027420.t1 AT3G19400 48.133 241 122 3 39 277 44 283 3.87e-76 236
MS.gene027420.t1 AT3G48350 51.793 251 112 7 32 277 31 277 4.72e-76 236
MS.gene027420.t1 AT5G60360 43.182 308 158 10 41 343 61 356 8.15e-71 224
MS.gene027420.t1 AT3G45310 42.258 310 160 11 41 344 61 357 1.64e-68 219
MS.gene027420.t1 AT5G60360 42.857 308 158 11 41 343 61 355 3.04e-68 218
MS.gene027420.t1 AT5G60360 42.282 298 159 9 41 333 61 350 1.63e-66 214
MS.gene027420.t1 AT3G45310 41.613 310 161 11 41 344 61 356 5.23e-66 212
MS.gene027420.t1 AT4G39090 39.048 315 159 11 45 340 57 357 4.22e-63 205
MS.gene027420.t1 AT2G21430 39.286 308 158 11 45 335 54 349 9.06e-63 204
MS.gene027420.t1 AT4G16190 39.048 315 163 10 44 340 60 363 7.36e-61 199
MS.gene027420.t1 AT3G54940 37.500 320 163 10 38 335 50 354 6.36e-59 194
MS.gene027420.t1 AT3G54940 36.910 233 130 5 38 259 50 276 3.83e-43 150
MS.gene027420.t1 AT1G02305 29.362 235 134 8 126 335 106 333 2.02e-23 99.8
MS.gene027420.t1 AT4G01610 27.801 241 142 8 120 335 97 330 6.90e-20 89.7
MS.gene027420.t1 AT1G02300 29.717 212 121 7 145 335 146 350 1.73e-19 89.0
MS.gene027420.t1 AT4G01610 27.386 241 143 8 120 335 97 330 9.99e-19 86.7
MS.gene027420.t1 AT2G22160 41.304 92 52 1 54 145 9 98 3.81e-15 70.9

Find 86 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATAAAGAGATCAACTTTC+TGG 0.194734 4.4:-53433464 None:intergenic
ATTGGCATTGCTTCTATGTT+TGG 0.245347 4.4:+53432905 MS.gene027420:CDS
AACACCTTGATAAGGGTAAT+TGG 0.282835 4.4:-53433599 None:intergenic
TTGGCATTGCTTCTATGTTT+GGG 0.292590 4.4:+53432906 MS.gene027420:CDS
AGGTTGTGAGGGCGGTCTTA+TGG 0.306810 4.4:+53433528 MS.gene027420:CDS
TCAGTATGGTAAAGTTTATA+AGG 0.313351 4.4:+53433004 MS.gene027420:CDS
AATATGATATAAGTGATTAT+TGG 0.319024 4.4:-53432880 None:intergenic
AAATTCATCATTCAAAATCA+TGG 0.364764 4.4:+53433562 MS.gene027420:CDS
GAACTCTTGGGGAACCGATT+GGG 0.373659 4.4:+53433885 MS.gene027420:CDS
ATCAACGCAGTTACCATTAC+TGG 0.386273 4.4:+53433652 MS.gene027420:CDS
TCACTTATATCATATTTCAT+TGG 0.405472 4.4:+53432887 MS.gene027420:CDS
ATTGGTTGGTTAAGAACTCT+TGG 0.408910 4.4:+53433872 MS.gene027420:CDS
TTGTAGTGCCTGCTCATTGT+TGG 0.422084 4.4:-53433692 None:intergenic
AGTAATGGTAACTGCGTTGA+TGG 0.423400 4.4:-53433650 None:intergenic
TTGGTTGGTTAAGAACTCTT+GGG 0.431188 4.4:+53433873 MS.gene027420:CDS
GCATCAATGGCTACAGAAAT+CGG 0.431804 4.4:-53433733 None:intergenic
AGTCAATTTATGAATTCCTT+CGG 0.435278 4.4:-53433440 None:intergenic
CATACTGACTCATCCATTGC+TGG 0.446987 4.4:-53432989 None:intergenic
CAGCAATGGATGAGTCAGTA+TGG 0.455594 4.4:+53432990 MS.gene027420:CDS
TTCCGTCAACACCTTGATAA+GGG 0.456935 4.4:-53433606 None:intergenic
GTCAGATCCACTAGCATCAA+TGG 0.459170 4.4:-53433746 None:intergenic
GTTCCGTCAACACCTTGATA+AGG 0.464383 4.4:-53433607 None:intergenic
TTGATAAGGGTAATTGGCTT+CGG 0.466155 4.4:-53433593 None:intergenic
TACCCTTATCAAGGTGTTGA+CGG 0.470988 4.4:+53433604 MS.gene027420:CDS
TTTATAAGGATTCTCAAGAA+AGG 0.473803 4.4:+53433018 MS.gene027420:CDS
TCTCATACATGGAGTCATCT+TGG 0.474251 4.4:-53432962 None:intergenic
CAACTCAGTTCCGCATGAAC+CGG 0.479102 4.4:-53433797 None:intergenic
TTGCTGGTGCCTCTCATACA+TGG 0.480269 4.4:-53432973 None:intergenic
TTCCTCCAATCCACTGAGGA+TGG 0.487802 4.4:-53433251 None:intergenic
TTATAAGGATTCTCAAGAAA+GGG 0.488285 4.4:+53433019 MS.gene027420:CDS
CAATTCCATCCTCAGTGGAT+TGG 0.490367 4.4:+53433246 MS.gene027420:CDS
GTATCCTTCTTCACCCCAAT+CGG 0.492451 4.4:-53433899 None:intergenic
AGAACTCTTGGGGAACCGAT+TGG 0.493995 4.4:+53433884 MS.gene027420:CDS
CATTGGCCTTGATTCTTCAC+CGG 0.499810 4.4:-53433290 None:intergenic
ATCGTCTAGAAACAAATTCA+AGG 0.505283 4.4:+53433166 MS.gene027420:CDS
AGTGCCTGCTCATTGTTGGC+CGG 0.505953 4.4:-53433688 None:intergenic
TTCCATCCTCAGTGGATTGG+AGG 0.507592 4.4:+53433249 MS.gene027420:CDS
GGTGTTACCGCTGTGGGTTA+CGG 0.510469 4.4:+53433826 MS.gene027420:CDS
TACATTGAAGCTTTCAACAA+AGG 0.517957 4.4:+53433077 MS.gene027420:CDS
GGAACCGATTGGGGTGAAGA+AGG 0.521876 4.4:+53433895 MS.gene027420:CDS
AACTCTTGGGGAACCGATTG+GGG 0.523168 4.4:+53433886 MS.gene027420:CDS
CGGTTCATGCGGAACTGAGT+TGG 0.529631 4.4:+53433798 MS.gene027420:CDS
TCAGTGGATTGGAGGAAGAA+AGG 0.529739 4.4:+53433257 MS.gene027420:CDS
TCTGTAGCCATTGATGCTAG+TGG 0.531179 4.4:+53433739 MS.gene027420:CDS
GACTTTCAATTCTACAAAAG+CGG 0.531410 4.4:+53433766 MS.gene027420:CDS
GTGACGACAGAACTAAGTAT+TGG 0.537741 4.4:+53433854 MS.gene027420:CDS
GTGCCTGCTCATTGTTGGCC+GGG 0.539134 4.4:-53433687 None:intergenic
TACTGGGTATGAAGATGTCC+CGG 0.540326 4.4:+53433669 MS.gene027420:CDS
ACTAGGCATTAATCAATTTG+CGG 0.545109 4.4:+53433121 MS.gene027420:CDS
CGACAGAACTAAGTATTGGT+TGG 0.545793 4.4:+53433858 MS.gene027420:CDS
TGTATGAGAGGCACCAGCAA+TGG 0.552809 4.4:+53432976 MS.gene027420:CDS
GTTACACCGGTGAAGAATCA+AGG 0.555167 4.4:+53433284 MS.gene027420:CDS
GATGCTGCTGAAGGAATTTG+TGG 0.557209 4.4:+53433943 MS.gene027420:CDS
TCTGCTGTTGCAGCAACCGA+AGG 0.563495 4.4:+53433424 MS.gene027420:CDS
ATGTGAACTCGTCGTTGGTG+AGG 0.564366 4.4:-53433145 None:intergenic
TAAGTGATTATTGGCAGCCA+TGG 0.564524 4.4:-53432871 None:intergenic
TGCGGAACTGAGTTGGATCA+TGG 0.566419 4.4:+53433805 MS.gene027420:CDS
AGACGATGTGAACTCGTCGT+TGG 0.573339 4.4:-53433150 None:intergenic
TCGTCTAGAAACAAATTCAA+GGG 0.573612 4.4:+53433167 MS.gene027420:CDS
GAAGCCAATTACCCTTATCA+AGG 0.574336 4.4:+53433595 MS.gene027420:CDS
GTGGACCAAGGTTGTGAGGG+CGG 0.578637 4.4:+53433520 MS.gene027420:CDS
TGTCCCGGCCAACAATGAGC+AGG 0.579548 4.4:+53433684 MS.gene027420:CDS
TAAGACCGCCCTCACAACCT+TGG 0.579957 4.4:-53433525 None:intergenic
TGGTTGGTTAAGAACTCTTG+GGG 0.580774 4.4:+53433874 MS.gene027420:CDS
AGGTGTGGACCAAGGTTGTG+AGG 0.589515 4.4:+53433516 MS.gene027420:CDS
GATCATGGTGTTACCGCTGT+GGG 0.595355 4.4:+53433820 MS.gene027420:CDS
GACATCTTCATACCCAGTAA+TGG 0.599023 4.4:-53433665 None:intergenic
AGAGGAGTTGATGCTGCTGA+AGG 0.602244 4.4:+53433934 MS.gene027420:CDS
AATAACAAGCTATACGCACT+AGG 0.605798 4.4:+53433104 MS.gene027420:CDS
GGATCATGGTGTTACCGCTG+TGG 0.606355 4.4:+53433819 MS.gene027420:CDS
GGTGTGGACCAAGGTTGTGA+GGG 0.610392 4.4:+53433517 MS.gene027420:CDS
TCAACGCAGTTACCATTACT+GGG 0.618651 4.4:+53433653 MS.gene027420:CDS
GGAACATGCAATGCAAACAA+AGG 0.625136 4.4:+53433625 MS.gene027420:CDS
CTCGTTGATTGTGACACGAA+AGG 0.633361 4.4:+53433496 MS.gene027420:CDS
GAGACGAAAACGTACCACAT+TGG 0.642550 4.4:-53433307 None:intergenic
AAGATGACTCCATGTATGAG+AGG 0.643922 4.4:+53432964 MS.gene027420:CDS
ATATGTGCTCTTCAATCACA+AGG 0.656906 4.4:+53433192 MS.gene027420:CDS
GAAGAAAGGAGCAGTTACAC+CGG 0.658192 4.4:+53433271 MS.gene027420:CDS
CGTCTAGAAACAAATTCAAG+GGG 0.665171 4.4:+53433168 MS.gene027420:CDS
AAGTGCAATTCCATCCTCAG+TGG 0.672799 4.4:+53433241 MS.gene027420:CDS
TTTCTTCCTCCAATCCACTG+AGG 0.673228 4.4:-53433255 None:intergenic
GGATACATTATGATGCAAAG+AGG 0.676444 4.4:+53433916 MS.gene027420:CDS
GACACGAAAGGTGTGGACCA+AGG 0.716503 4.4:+53433508 MS.gene027420:CDS
TGATTGTGACACGAAAGGTG+TGG 0.726786 4.4:+53433501 MS.gene027420:CDS
ACTAACACCGTAACCCACAG+CGG 0.728918 4.4:-53433833 None:intergenic
GTGAAGAATCAAGGCCAATG+TGG 0.733517 4.4:+53433293 MS.gene027420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGATATAAGTGATTAT+TGG - chr4.4:53432883-53432902 None:intergenic 15.0%
!!! AGTTTTAATTTACATTTTGT+AGG + chr4.4:53433385-53433404 MS.gene027420:intron 15.0%
!! AAATTCATCATTCAAAATCA+TGG + chr4.4:53433562-53433581 MS.gene027420:CDS 20.0%
!! TCACTTATATCATATTTCAT+TGG + chr4.4:53432887-53432906 MS.gene027420:CDS 20.0%
! AGTCAATTTATGAATTCCTT+CGG - chr4.4:53433443-53433462 None:intergenic 25.0%
! TCAGTATGGTAAAGTTTATA+AGG + chr4.4:53433004-53433023 MS.gene027420:CDS 25.0%
! TTATAAGGATTCTCAAGAAA+GGG + chr4.4:53433019-53433038 MS.gene027420:CDS 25.0%
! TTTATAAGGATTCTCAAGAA+AGG + chr4.4:53433018-53433037 MS.gene027420:CDS 25.0%
ACTAGGCATTAATCAATTTG+CGG + chr4.4:53433121-53433140 MS.gene027420:CDS 30.0%
ATCGTCTAGAAACAAATTCA+AGG + chr4.4:53433166-53433185 MS.gene027420:CDS 30.0%
GACTTTCAATTCTACAAAAG+CGG + chr4.4:53433766-53433785 MS.gene027420:CDS 30.0%
TACATTGAAGCTTTCAACAA+AGG + chr4.4:53433077-53433096 MS.gene027420:CDS 30.0%
TCGTCTAGAAACAAATTCAA+GGG + chr4.4:53433167-53433186 MS.gene027420:CDS 30.0%
TGATAAAGAGATCAACTTTC+TGG - chr4.4:53433467-53433486 None:intergenic 30.0%
! ACATTTTGTAGGATGTTGTT+GGG + chr4.4:53433396-53433415 MS.gene027420:intron 30.0%
! TACATTTTGTAGGATGTTGT+TGG + chr4.4:53433395-53433414 MS.gene027420:intron 30.0%
AACACCTTGATAAGGGTAAT+TGG - chr4.4:53433602-53433621 None:intergenic 35.0%
AATAACAAGCTATACGCACT+AGG + chr4.4:53433104-53433123 MS.gene027420:CDS 35.0%
ATATGTGCTCTTCAATCACA+AGG + chr4.4:53433192-53433211 MS.gene027420:CDS 35.0%
ATTGGCATTGCTTCTATGTT+TGG + chr4.4:53432905-53432924 MS.gene027420:CDS 35.0%
CGTCTAGAAACAAATTCAAG+GGG + chr4.4:53433168-53433187 MS.gene027420:CDS 35.0%
GGATACATTATGATGCAAAG+AGG + chr4.4:53433916-53433935 MS.gene027420:CDS 35.0%
TTGGCATTGCTTCTATGTTT+GGG + chr4.4:53432906-53432925 MS.gene027420:CDS 35.0%
! ATTGGTTGGTTAAGAACTCT+TGG + chr4.4:53433872-53433891 MS.gene027420:CDS 35.0%
! TTGATAAGGGTAATTGGCTT+CGG - chr4.4:53433596-53433615 None:intergenic 35.0%
! TTGGTTGGTTAAGAACTCTT+GGG + chr4.4:53433873-53433892 MS.gene027420:CDS 35.0%
AAGATGACTCCATGTATGAG+AGG + chr4.4:53432964-53432983 MS.gene027420:CDS 40.0%
AGTAATGGTAACTGCGTTGA+TGG - chr4.4:53433653-53433672 None:intergenic 40.0%
ATCAACGCAGTTACCATTAC+TGG + chr4.4:53433652-53433671 MS.gene027420:CDS 40.0%
GAAGCCAATTACCCTTATCA+AGG + chr4.4:53433595-53433614 MS.gene027420:CDS 40.0%
GACATCTTCATACCCAGTAA+TGG - chr4.4:53433668-53433687 None:intergenic 40.0%
GCATCAATGGCTACAGAAAT+CGG - chr4.4:53433736-53433755 None:intergenic 40.0%
GGAACATGCAATGCAAACAA+AGG + chr4.4:53433625-53433644 MS.gene027420:CDS 40.0%
GTGACGACAGAACTAAGTAT+TGG + chr4.4:53433854-53433873 MS.gene027420:CDS 40.0%
TCAACGCAGTTACCATTACT+GGG + chr4.4:53433653-53433672 MS.gene027420:CDS 40.0%
TCTCATACATGGAGTCATCT+TGG - chr4.4:53432965-53432984 None:intergenic 40.0%
TTCCGTCAACACCTTGATAA+GGG - chr4.4:53433609-53433628 None:intergenic 40.0%
! TACAAAAGCGGTGTTTTCAC+CGG + chr4.4:53433778-53433797 MS.gene027420:CDS 40.0%
! TGGTTGGTTAAGAACTCTTG+GGG + chr4.4:53433874-53433893 MS.gene027420:CDS 40.0%
!! CGACAGAACTAAGTATTGGT+TGG + chr4.4:53433858-53433877 MS.gene027420:CDS 40.0%
!! TACCCTTATCAAGGTGTTGA+CGG + chr4.4:53433604-53433623 MS.gene027420:CDS 40.0%
AAGTGCAATTCCATCCTCAG+TGG + chr4.4:53433241-53433260 MS.gene027420:CDS 45.0%
CAATTCCATCCTCAGTGGAT+TGG + chr4.4:53433246-53433265 MS.gene027420:CDS 45.0%
CAGCAATGGATGAGTCAGTA+TGG + chr4.4:53432990-53433009 MS.gene027420:CDS 45.0%
GAAGAAAGGAGCAGTTACAC+CGG + chr4.4:53433271-53433290 MS.gene027420:CDS 45.0%
GAGACGAAAACGTACCACAT+TGG - chr4.4:53433310-53433329 None:intergenic 45.0%
GATGCTGCTGAAGGAATTTG+TGG + chr4.4:53433943-53433962 MS.gene027420:CDS 45.0%
GTATCCTTCTTCACCCCAAT+CGG - chr4.4:53433902-53433921 None:intergenic 45.0%
GTCAGATCCACTAGCATCAA+TGG - chr4.4:53433749-53433768 None:intergenic 45.0%
GTGAAGAATCAAGGCCAATG+TGG + chr4.4:53433293-53433312 MS.gene027420:CDS 45.0%
GTTACACCGGTGAAGAATCA+AGG + chr4.4:53433284-53433303 MS.gene027420:CDS 45.0%
GTTCCGTCAACACCTTGATA+AGG - chr4.4:53433610-53433629 None:intergenic 45.0%
TACTGGGTATGAAGATGTCC+CGG + chr4.4:53433669-53433688 MS.gene027420:CDS 45.0%
TCTGTAGCCATTGATGCTAG+TGG + chr4.4:53433739-53433758 MS.gene027420:CDS 45.0%
TGATTGTGACACGAAAGGTG+TGG + chr4.4:53433501-53433520 MS.gene027420:CDS 45.0%
TTGTAGTGCCTGCTCATTGT+TGG - chr4.4:53433695-53433714 None:intergenic 45.0%
TTTCTTCCTCCAATCCACTG+AGG - chr4.4:53433258-53433277 None:intergenic 45.0%
! CTCGTTGATTGTGACACGAA+AGG + chr4.4:53433496-53433515 MS.gene027420:CDS 45.0%
!! CATACTGACTCATCCATTGC+TGG - chr4.4:53432992-53433011 None:intergenic 45.0%
!! CATTGGCCTTGATTCTTCAC+CGG - chr4.4:53433293-53433312 None:intergenic 45.0%
!! TCAGTGGATTGGAGGAAGAA+AGG + chr4.4:53433257-53433276 MS.gene027420:CDS 45.0%
AACTCTTGGGGAACCGATTG+GGG + chr4.4:53433886-53433905 MS.gene027420:CDS 50.0%
ACTAACACCGTAACCCACAG+CGG - chr4.4:53433836-53433855 None:intergenic 50.0%
AGAACTCTTGGGGAACCGAT+TGG + chr4.4:53433884-53433903 MS.gene027420:CDS 50.0%
AGACGATGTGAACTCGTCGT+TGG - chr4.4:53433153-53433172 None:intergenic 50.0%
ATGTGAACTCGTCGTTGGTG+AGG - chr4.4:53433148-53433167 None:intergenic 50.0%
CAACTCAGTTCCGCATGAAC+CGG - chr4.4:53433800-53433819 None:intergenic 50.0%
GAACTCTTGGGGAACCGATT+GGG + chr4.4:53433885-53433904 MS.gene027420:CDS 50.0%
GCAGGCACTACAAAAAGCAG+TGG + chr4.4:53433702-53433721 MS.gene027420:CDS 50.0%
TTCCTCCAATCCACTGAGGA+TGG - chr4.4:53433254-53433273 None:intergenic 50.0%
TTGCTGGTGCCTCTCATACA+TGG - chr4.4:53432976-53432995 None:intergenic 50.0%
! TGCGGAACTGAGTTGGATCA+TGG + chr4.4:53433805-53433824 MS.gene027420:CDS 50.0%
!! AGAGGAGTTGATGCTGCTGA+AGG + chr4.4:53433934-53433953 MS.gene027420:CDS 50.0%
!! GATCATGGTGTTACCGCTGT+GGG + chr4.4:53433820-53433839 MS.gene027420:CDS 50.0%
!! TGTATGAGAGGCACCAGCAA+TGG + chr4.4:53432976-53432995 MS.gene027420:CDS 50.0%
!! TTCCATCCTCAGTGGATTGG+AGG + chr4.4:53433249-53433268 MS.gene027420:CDS 50.0%
!!! GGTGTTTTCACCGGTTCATG+CGG + chr4.4:53433787-53433806 MS.gene027420:CDS 50.0%
AGGTGTGGACCAAGGTTGTG+AGG + chr4.4:53433516-53433535 MS.gene027420:CDS 55.0%
AGGTTGTGAGGGCGGTCTTA+TGG + chr4.4:53433528-53433547 MS.gene027420:CDS 55.0%
AGTGCCTGCTCATTGTTGGC+CGG - chr4.4:53433691-53433710 None:intergenic 55.0%
CGGTTCATGCGGAACTGAGT+TGG + chr4.4:53433798-53433817 MS.gene027420:CDS 55.0%
GGAACCGATTGGGGTGAAGA+AGG + chr4.4:53433895-53433914 MS.gene027420:CDS 55.0%
GGTGTGGACCAAGGTTGTGA+GGG + chr4.4:53433517-53433536 MS.gene027420:CDS 55.0%
TAAGACCGCCCTCACAACCT+TGG - chr4.4:53433528-53433547 None:intergenic 55.0%
TCTGCTGTTGCAGCAACCGA+AGG + chr4.4:53433424-53433443 MS.gene027420:CDS 55.0%
! GACACGAAAGGTGTGGACCA+AGG + chr4.4:53433508-53433527 MS.gene027420:CDS 55.0%
! GGTGTTACCGCTGTGGGTTA+CGG + chr4.4:53433826-53433845 MS.gene027420:CDS 55.0%
!! GGATCATGGTGTTACCGCTG+TGG + chr4.4:53433819-53433838 MS.gene027420:CDS 55.0%
GTGCCTGCTCATTGTTGGCC+GGG - chr4.4:53433690-53433709 None:intergenic 60.0%
GTGGACCAAGGTTGTGAGGG+CGG + chr4.4:53433520-53433539 MS.gene027420:CDS 60.0%
TGTCCCGGCCAACAATGAGC+AGG + chr4.4:53433684-53433703 MS.gene027420:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 53432873 53433999 53432873 ID=MS.gene027420
chr4.4 mRNA 53432873 53433999 53432873 ID=MS.gene027420.t1;Parent=MS.gene027420
chr4.4 exon 53432873 53433314 53432873 ID=MS.gene027420.t1.exon1;Parent=MS.gene027420.t1
chr4.4 CDS 53432873 53433314 53432873 ID=cds.MS.gene027420.t1;Parent=MS.gene027420.t1
chr4.4 exon 53433407 53433999 53433407 ID=MS.gene027420.t1.exon2;Parent=MS.gene027420.t1
chr4.4 CDS 53433407 53433999 53433407 ID=cds.MS.gene027420.t1;Parent=MS.gene027420.t1
Gene Sequence

>MS.gene027420

ATGGCTGCCAATAATCACTTATATCATATTTCATTGGCATTGCTTCTATGTTTGGGATTGTTTGCGATTCAAGTCACTTCTCGCACTCTCCAAGATGACTCCATGTATGAGAGGCACCAGCAATGGATGAGTCAGTATGGTAAAGTTTATAAGGATTCTCAAGAAAGGGAGAAGCGTTTCAAGATATTCACTGAAAACGTGAATTACATTGAAGCTTTCAACAAAGGTGACAATAACAAGCTATACGCACTAGGCATTAATCAATTTGCGGACCTCACCAACGACGAGTTCACATCGTCTAGAAACAAATTCAAGGGGCATATGTGCTCTTCAATCACAAGGACAAGTACTTTTAAGTATGAAAATACAAGTGCAATTCCATCCTCAGTGGATTGGAGGAAGAAAGGAGCAGTTACACCGGTGAAGAATCAAGGCCAATGTGGTACGTTTTCGTCTCATAATGCATTCGATTTTATATGTGATAAAAAATTAATTATCGTCTAACTCTAGTTAGTTTTAATTTACATTTTGTAGGATGTTGTTGGGCGTTTTCTGCTGTTGCAGCAACCGAAGGAATTCATAAATTGACTACCAGAAAGTTGATCTCTTTATCAGAACAAGAACTCGTTGATTGTGACACGAAAGGTGTGGACCAAGGTTGTGAGGGCGGTCTTATGGACGATGCTTTCAAATTCATCATTCAAAATCATGGACTCGCTACCGAAGCCAATTACCCTTATCAAGGTGTTGACGGAACATGCAATGCAAACAAAGGATCCATCAACGCAGTTACCATTACTGGGTATGAAGATGTCCCGGCCAACAATGAGCAGGCACTACAAAAAGCAGTGGCTAATCAACCGATTTCTGTAGCCATTGATGCTAGTGGATCTGACTTTCAATTCTACAAAAGCGGTGTTTTCACCGGTTCATGCGGAACTGAGTTGGATCATGGTGTTACCGCTGTGGGTTACGGTGTTAGTGACGACAGAACTAAGTATTGGTTGGTTAAGAACTCTTGGGGAACCGATTGGGGTGAAGAAGGATACATTATGATGCAAAGAGGAGTTGATGCTGCTGAAGGAATTTGTGGCATTGCAATGCAAGCATCATACCCTACTGCTTAA
Protein sequence

>MS.gene027420.t1

MAANNHLYHISLALLLCLGLFAIQVTSRTLQDDSMYERHQQWMSQYGKVYKDSQEREKRFKIFTENVNYIEAFNKGDNNKLYALGINQFADLTNDEFTSSRNKFKGHMCSSITRTSTFKYENTSAIPSSVDWRKKGAVTPVKNQGQCGCCWAFSAVAATEGIHKLTTRKLISLSEQELVDCDTKGVDQGCEGGLMDDAFKFIIQNHGLATEANYPYQGVDGTCNANKGSINAVTITGYEDVPANNEQALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGVSDDRTKYWLVKNSWGTDWGEEGYIMMQRGVDAAEGICGIAMQASYPTA