AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031581

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031581.t1 MTR_4g080700 94.412 340 19 0 1 340 1 340 0.0 650
MS.gene031581.t1 MTR_4g080730 92.941 340 24 0 1 340 1 340 0.0 640
MS.gene031581.t1 MTR_5g022560 63.582 335 115 6 11 340 12 344 1.87e-155 439
MS.gene031581.t1 MTR_4g107930 61.494 348 123 5 1 340 1 345 1.60e-154 437
MS.gene031581.t1 MTR_4g079770 64.286 336 111 8 11 340 13 345 4.73e-154 436
MS.gene031581.t1 MTR_4g080360 61.224 343 125 5 4 340 2 342 8.12e-154 435
MS.gene031581.t1 MTR_4g047610 64.798 321 106 6 25 340 25 343 2.41e-152 432
MS.gene031581.t1 MTR_4g079470 64.907 322 105 7 25 340 26 345 1.57e-151 430
MS.gene031581.t1 MTR_4g079800 64.174 321 109 6 25 340 26 345 5.36e-151 428
MS.gene031581.t1 MTR_2g075830 61.516 343 127 5 1 340 1 341 2.51e-150 426
MS.gene031581.t1 MTR_2g083930 60.058 343 120 6 6 338 7 342 4.07e-139 398
MS.gene031581.t1 MTR_3g075390 54.006 337 144 7 10 338 10 343 1.05e-126 367
MS.gene031581.t1 MTR_7g078600 59.091 308 118 5 33 338 35 336 1.67e-126 366
MS.gene031581.t1 MTR_4g104370 55.060 336 139 8 11 338 11 342 3.06e-125 363
MS.gene031581.t1 MTR_7g079230 57.419 310 125 5 32 339 31 335 4.80e-124 360
MS.gene031581.t1 MTR_1g107550 54.839 310 132 4 36 338 46 354 2.29e-118 350
MS.gene031581.t1 MTR_3g116080 52.381 336 150 6 11 339 15 347 2.60e-117 343
MS.gene031581.t1 MTR_2g090890 48.788 330 165 3 10 338 9 335 9.31e-116 338
MS.gene031581.t1 MTR_4g079440 48.512 336 161 4 13 338 10 343 2.35e-114 335
MS.gene031581.t1 MTR_4g094918 47.761 335 164 6 14 339 9 341 4.68e-113 332
MS.gene031581.t1 MTR_3g102220 48.895 362 159 9 1 338 1 360 2.69e-112 335
MS.gene031581.t1 MTR_1g018840 51.948 308 137 6 40 338 55 360 5.71e-108 323
MS.gene031581.t1 MTR_1g018840 51.948 308 137 6 40 338 55 360 8.02e-108 323
MS.gene031581.t1 MTR_3g112430 49.346 306 147 3 41 339 37 341 1.54e-101 306
MS.gene031581.t1 MTR_2g016180 44.083 338 172 8 16 339 21 355 3.69e-86 268
MS.gene031581.t1 MTR_2g015850 48.110 291 139 7 55 338 77 362 1.22e-84 260
MS.gene031581.t1 MTR_2g016270 44.048 336 160 9 16 339 21 340 3.25e-84 263
MS.gene031581.t1 MTR_2g067900 45.201 323 157 8 30 337 6 323 2.33e-79 245
MS.gene031581.t1 MTR_2g064475 41.274 361 155 13 26 339 1 351 2.10e-77 241
MS.gene031581.t1 MTR_7g078630 47.601 271 104 5 36 295 702 945 6.93e-72 240
MS.gene031581.t1 MTR_4g065077 37.088 364 196 12 1 339 1 356 7.14e-71 225
MS.gene031581.t1 MTR_4g125300 40.803 299 165 7 46 339 57 348 2.62e-70 223
MS.gene031581.t1 MTR_2g015920 39.441 322 178 9 23 331 33 350 3.55e-70 223
MS.gene031581.t1 MTR_2g067560 42.296 331 160 11 26 333 1 323 7.93e-69 231
MS.gene031581.t1 MTR_2g015930 38.439 346 181 14 10 331 13 350 1.83e-66 213
MS.gene031581.t1 MTR_2g066980 36.338 355 166 11 7 339 2 318 2.30e-66 212
MS.gene031581.t1 MTR_4g071060 35.143 350 191 9 11 331 6 348 5.97e-65 209
MS.gene031581.t1 MTR_8g086470 35.537 363 187 12 1 339 17 356 1.93e-63 206
MS.gene031581.t1 MTR_1g023210 35.393 356 191 12 8 331 4 352 1.63e-61 201
MS.gene031581.t1 MTR_1g023810 45.570 237 118 8 107 337 30 261 4.46e-58 189
MS.gene031581.t1 MTR_1g094135 34.734 357 183 11 9 331 18 358 1.51e-56 188
MS.gene031581.t1 MTR_4g072790 32.432 333 181 9 10 331 13 312 1.17e-47 164
MS.gene031581.t1 MTR_4g072340 33.038 339 175 9 10 331 13 316 1.57e-46 160
MS.gene031581.t1 MTR_4g073660 31.940 335 184 8 10 331 12 315 2.86e-46 160
MS.gene031581.t1 MTR_7g079130 70.000 90 26 1 250 338 7 96 8.75e-39 133
MS.gene031581.t1 MTR_7g078580 67.010 97 30 2 243 338 5 100 1.43e-37 131
MS.gene031581.t1 MTR_2g015840 42.675 157 83 3 8 159 2 156 3.28e-36 132
MS.gene031581.t1 MTR_2g015840 49.550 111 54 2 230 339 162 271 1.47e-30 117
MS.gene031581.t1 MTR_1g025450 41.361 191 88 5 107 292 36 207 1.60e-34 133
MS.gene031581.t1 MTR_7g078310 44.928 138 69 3 82 217 115 247 9.66e-31 117
MS.gene031581.t1 MTR_2g016380 46.552 116 58 2 227 339 21 135 8.03e-27 106
MS.gene031581.t1 MTR_7g111060 28.571 273 162 12 89 334 67 333 9.78e-23 97.8
MS.gene031581.t1 MTR_2g047960 53.750 80 36 1 133 212 7 85 4.14e-21 87.0
MS.gene031581.t1 MTR_7g111050 29.825 228 132 9 126 331 106 327 9.81e-20 89.4
MS.gene031581.t1 MTR_3g029560 28.365 208 123 5 136 338 50 236 1.30e-18 84.0
MS.gene031581.t1 MTR_7g079260 55.556 72 15 2 266 336 7 62 4.67e-18 79.0
MS.gene031581.t1 MTR_8g087510 33.077 130 81 4 165 292 23 148 8.23e-17 77.4
MS.gene031581.t1 MTR_7g111050 27.273 198 120 7 126 301 101 296 2.30e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031581.t1 AT5G45890 52.632 342 147 7 11 339 6 345 4.20e-128 370
MS.gene031581.t1 AT5G50260 55.685 343 139 8 6 338 2 341 8.50e-127 367
MS.gene031581.t1 AT3G48340 51.297 347 149 7 6 338 2 342 3.23e-118 346
MS.gene031581.t1 AT5G43060 52.890 346 141 7 13 338 9 352 3.72e-117 347
MS.gene031581.t1 AT3G19390 50.000 348 161 6 2 339 1 345 1.66e-115 342
MS.gene031581.t1 AT3G19400 52.787 305 139 3 40 339 44 348 1.03e-113 334
MS.gene031581.t1 AT2G34080 46.802 344 166 7 10 340 6 345 1.05e-113 333
MS.gene031581.t1 AT2G27420 48.266 346 161 7 9 340 7 348 3.76e-113 332
MS.gene031581.t1 AT3G48350 49.422 346 155 8 11 339 1 343 9.85e-113 332
MS.gene031581.t1 AT4G35350 51.242 322 151 4 22 339 33 352 1.70e-112 331
MS.gene031581.t1 AT1G29090 47.813 343 166 7 9 340 15 355 6.33e-112 330
MS.gene031581.t1 AT1G20850 53.398 309 137 5 36 339 47 353 1.74e-111 328
MS.gene031581.t1 AT1G47128 53.289 304 135 5 40 338 50 351 1.75e-111 332
MS.gene031581.t1 AT3G49340 48.246 342 160 8 9 340 7 341 7.36e-110 324
MS.gene031581.t1 AT4G23520 48.387 341 161 7 10 338 11 348 5.26e-108 320
MS.gene031581.t1 AT4G11310 49.836 305 143 6 41 338 50 351 2.55e-107 318
MS.gene031581.t1 AT4G36880 52.244 312 132 8 42 338 51 360 3.63e-107 318
MS.gene031581.t1 AT1G29080 45.000 340 168 7 13 340 14 346 1.32e-106 316
MS.gene031581.t1 AT4G11320 49.508 305 144 6 41 338 57 358 1.06e-105 314
MS.gene031581.t1 AT4G11320 49.508 305 144 6 41 338 57 358 1.06e-105 314
MS.gene031581.t1 AT1G09850 51.140 307 144 4 36 338 28 332 4.03e-104 312
MS.gene031581.t1 AT1G06260 47.734 331 168 5 10 338 14 341 1.41e-103 308
MS.gene031581.t1 AT3G43960 48.534 307 148 6 40 338 41 345 2.85e-91 278
MS.gene031581.t1 AT1G29110 39.017 346 187 6 4 339 2 333 1.91e-89 271
MS.gene031581.t1 AT3G19400 50.633 237 112 3 40 271 44 280 5.36e-78 241
MS.gene031581.t1 AT4G35350 47.287 258 129 5 22 275 33 287 1.04e-74 232
MS.gene031581.t1 AT3G48350 45.714 280 133 7 11 274 1 277 5.96e-74 231
MS.gene031581.t1 AT5G60360 40.764 314 166 9 37 339 52 356 3.92e-71 225
MS.gene031581.t1 AT3G45310 39.103 312 174 8 37 339 52 356 9.10e-69 219
MS.gene031581.t1 AT5G60360 40.446 314 166 10 37 339 52 355 1.50e-68 219
MS.gene031581.t1 AT5G60360 40.328 305 162 9 37 330 52 347 2.72e-67 216
MS.gene031581.t1 AT3G45310 38.782 312 174 9 37 339 52 355 3.60e-66 213
MS.gene031581.t1 AT2G21430 34.682 346 193 11 10 331 13 349 3.98e-64 207
MS.gene031581.t1 AT4G39090 37.582 306 161 9 46 331 57 352 4.67e-62 202
MS.gene031581.t1 AT3G54940 33.696 368 193 11 1 331 1 354 2.47e-57 190
MS.gene031581.t1 AT4G16190 35.197 304 173 8 46 331 61 358 2.50e-53 180
MS.gene031581.t1 AT3G54940 33.099 284 160 7 1 259 1 279 3.52e-42 148
MS.gene031581.t1 AT1G02305 28.522 291 174 11 69 331 49 333 2.22e-24 102
MS.gene031581.t1 AT4G01610 27.922 308 186 12 52 331 31 330 2.31e-24 102
MS.gene031581.t1 AT4G01610 27.273 308 188 12 52 331 31 330 2.54e-22 96.7
MS.gene031581.t1 AT1G02300 28.436 211 123 8 143 331 146 350 9.23e-18 84.0
MS.gene031581.t1 AT2G22160 43.011 93 44 3 56 143 10 98 9.86e-15 69.7

Find 81 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACATTGTCCTTGAATATT+TGG 0.087740 4.4:-31215131 None:intergenic
TTTGACTATTGAAGAATTTA+AGG 0.095428 4.4:+31215221 MS.gene031581:CDS
CTTCCATCAGCCGCTTTATA+AGG 0.201613 4.4:-31216016 None:intergenic
TTCCTCATCCTTGCACTTGC+TGG 0.223659 4.4:+31214988 MS.gene031581:CDS
GTGCTAATGGCACTGATTAT+TGG 0.243505 4.4:+31216257 MS.gene031581:CDS
ACCCCTGTGGCTAAGATTAA+AGG 0.261539 4.4:+31216058 MS.gene031581:CDS
AGCACTTTCTCGCTATTAAC+TGG 0.298226 4.4:-31216094 None:intergenic
CATTGACCCTGGTCCTTGAT+TGG 0.342967 4.4:-31215360 None:intergenic
ACATCCTTGATCTTCACCTT+TGG 0.353254 4.4:-31215921 None:intergenic
GCATCAATGGATACTGATAT+TGG 0.360718 4.4:-31216139 None:intergenic
GCTGTAGATTGGAGGTTAAA+AGG 0.362932 4.4:+31215327 MS.gene031581:CDS
CAATCTTCTTGTAACCATTA+CGG 0.367579 4.4:-31215248 None:intergenic
AATCTTCTTGTAACCATTAC+GGG 0.369381 4.4:-31215247 None:intergenic
GGTGTTACTGCTGTTGGTTA+TGG 0.376619 4.4:+31216232 MS.gene031581:CDS
TGATTAACACTTAGCTTGTA+AGG 0.378815 4.4:-31215189 None:intergenic
ATTGGATTGTGAAGAATTCA+TGG 0.385037 4.4:+31216275 MS.gene031581:CDS
GAACAATGGATGGTAGAATA+TGG 0.390228 4.4:+31215069 MS.gene031581:CDS
CTATTCCAGCAGCTGTAGAT+TGG 0.409369 4.4:+31215316 MS.gene031581:CDS
TTCCATCAGCCGCTTTATAA+GGG 0.422592 4.4:-31216015 None:intergenic
ATGAAACAAAAGGGAGCTGC+TGG 0.423861 4.4:+31215804 MS.gene031581:intron
AGGATGTGAAGGAGGACTAA+TGG 0.423869 4.4:+31215937 MS.gene031581:CDS
AACCTCCAATCTACAGCTGC+TGG 0.429672 4.4:-31215321 None:intergenic
GAATTTAAGGCTTCCCGTAA+TGG 0.442626 4.4:+31215234 MS.gene031581:CDS
ACTTGAATCACTAGCATCAA+TGG 0.443557 4.4:-31216152 None:intergenic
GTTGGTTATGGTAGTGCTAA+TGG 0.449761 4.4:+31216244 MS.gene031581:CDS
AATCTTAGCCACAGGGGTGG+TGG 0.453955 4.4:-31216053 None:intergenic
GGCACAGTGTGGGGTGAGAA+AGG 0.461177 4.4:+31216298 MS.gene031581:CDS
TTCAACAGTGGCTGCAACTG+AGG 0.464305 4.4:+31215832 MS.gene031581:CDS
GACCATGGTGTTACTGCTGT+TGG 0.465152 4.4:+31216226 MS.gene031581:CDS
GCAAAGAGGTATAGCTACTA+AGG 0.466603 4.4:+31216333 MS.gene031581:CDS
GGGGTGAGAAAGGGTACATA+AGG 0.472010 4.4:+31216308 MS.gene031581:CDS
AATCCTTTAATCTTAGCCAC+AGG 0.472271 4.4:-31216061 None:intergenic
TAAGATTAAAGGATTTGAAA+AGG 0.475936 4.4:+31216069 MS.gene031581:CDS
AACACGTTTCCAAATATTCA+AGG 0.489113 4.4:+31215122 MS.gene031581:CDS
AATTGAGCAGGTTAAGCAGT+TGG 0.502345 4.4:-31216391 None:intergenic
AGGAGCTGTCACTCCAATCA+AGG 0.506249 4.4:+31215347 MS.gene031581:CDS
TTCCAGCAGCTGTAGATTGG+AGG 0.518760 4.4:+31215319 MS.gene031581:CDS
TCGTGTCTTTCTTGTAAAGA+TGG 0.523194 4.4:-31215048 None:intergenic
GAATTCATGGGGCACAGTGT+GGG 0.529597 4.4:+31216288 MS.gene031581:CDS
GCACAGTGTGGGGTGAGAAA+GGG 0.530150 4.4:+31216299 MS.gene031581:CDS
TACCAGCAAGTGCAAGGATG+AGG 0.532312 4.4:-31214990 None:intergenic
TACCCTTATAAAGCGGCTGA+TGG 0.533723 4.4:+31216013 MS.gene031581:CDS
GAAGGAGGACTAATGGAAGA+TGG 0.537127 4.4:+31215944 MS.gene031581:CDS
CAATGGATACTGATATTGGT+TGG 0.538870 4.4:-31216135 None:intergenic
GGTATCAACCAAATAACTAC+AGG 0.541357 4.4:+31215854 MS.gene031581:CDS
TTGGATTGTGAAGAATTCAT+GGG 0.541633 4.4:+31216276 MS.gene031581:CDS
GCTACTAAGGAAGGCTTATG+TGG 0.549794 4.4:+31216346 MS.gene031581:CDS
TGTCACTCCAATCAAGGACC+AGG 0.555614 4.4:+31215353 MS.gene031581:CDS
AGGCTTATGTGGTATTGCCA+TGG 0.564267 4.4:+31216357 MS.gene031581:CDS
AACTAGCTCTTGTTCTGAGA+GGG 0.572252 4.4:-31215888 None:intergenic
TCAACAGTGGCTGCAACTGA+GGG 0.573583 4.4:+31215833 MS.gene031581:CDS
GTATGAAGATGCTGCTGAGA+AGG 0.577908 4.4:+31215098 MS.gene031581:CDS
AATTCATGGGGCACAGTGTG+GGG 0.578521 4.4:+31216289 MS.gene031581:CDS
AGAAAGACACGAACAATGGA+TGG 0.579345 4.4:+31215059 MS.gene031581:CDS
TTATCTTACCACATTGACCC+TGG 0.582806 4.4:-31215371 None:intergenic
AGAGGTATAGCTACTAAGGA+AGG 0.583530 4.4:+31216337 MS.gene031581:CDS
TGGTAATACCAGCAAGTGCA+AGG 0.584093 4.4:-31214996 None:intergenic
GGAATTTACACAGGAGAATG+TGG 0.588477 4.4:+31216193 MS.gene031581:CDS
TATTCTAGTGGAATTTACAC+AGG 0.589049 4.4:+31216184 MS.gene031581:CDS
GACACCAAAGGTGAAGATCA+AGG 0.593673 4.4:+31215917 MS.gene031581:CDS
AGAATTCATGGGGCACAGTG+TGG 0.594834 4.4:+31216287 MS.gene031581:CDS
ATCCTTTAATCTTAGCCACA+GGG 0.605196 4.4:-31216060 None:intergenic
GGTGAAGATCAAGGATGTGA+AGG 0.605528 4.4:+31215926 MS.gene031581:CDS
AGTTGGATAAGAAGAATCCA+TGG 0.617565 4.4:-31216374 None:intergenic
GAAAGTGCTTCTCAAAGCTG+TGG 0.624708 4.4:+31216108 MS.gene031581:CDS
GAAGATCAAGGATGTGAAGG+AGG 0.626945 4.4:+31215929 MS.gene031581:CDS
TGAAACAAAAGGGAGCTGCT+GGG 0.627368 4.4:+31215805 MS.gene031581:intron
GACTAATTACCCTTATAAAG+CGG 0.628662 4.4:+31216006 MS.gene031581:CDS
TACAAGAAAGACACGAACAA+TGG 0.636068 4.4:+31215055 MS.gene031581:CDS
CAACTAGCTCTTGTTCTGAG+AGG 0.636772 4.4:-31215889 None:intergenic
CTTCAATAGTCAAATCAGCA+AGG 0.637348 4.4:-31215212 None:intergenic
TTTAATCTTAGCCACAGGGG+TGG 0.638615 4.4:-31216056 None:intergenic
CACTGCAACCACCACCCCTG+TGG 0.644161 4.4:+31216045 MS.gene031581:CDS
TGGATTGTGAAGAATTCATG+GGG 0.646576 4.4:+31216277 MS.gene031581:CDS
ATCAAGGACCAGGGTCAATG+TGG 0.657892 4.4:+31215363 MS.gene031581:CDS
CTAGTTGATTGTGACACCAA+AGG 0.661401 4.4:+31215905 MS.gene031581:CDS
GTCACTCCAATCAAGGACCA+GGG 0.673826 4.4:+31215354 MS.gene031581:CDS
AACCAACAGCAGTAACACCA+TGG 0.692410 4.4:-31216228 None:intergenic
GGGTACATAAGGATGCAAAG+AGG 0.705777 4.4:+31216319 MS.gene031581:CDS
TCCTTTAATCTTAGCCACAG+GGG 0.712399 4.4:-31216059 None:intergenic
TGTGGAACTGAGCTAGACCA+TGG 0.784822 4.4:+31216211 MS.gene031581:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTTCATATAATAAAG+CGG - chr4.4:31215578-31215597 None:intergenic 10.0%
!! TTTGAATTTATCATTAAAAA+TGG + chr4.4:31215968-31215987 MS.gene031581:CDS 10.0%
!!! AGTTTATTAATTTTTTTTTG+AGG - chr4.4:31215670-31215689 None:intergenic 10.0%
!!! TAATAAACTTTTGTTATATA+TGG + chr4.4:31215681-31215700 MS.gene031581:intron 10.0%
!! TTATATTGAGTCTAATTAAT+TGG + chr4.4:31215743-31215762 MS.gene031581:intron 15.0%
!! ATTTATCCACGAAAATTATT+AGG - chr4.4:31215474-31215493 None:intergenic 20.0%
!! GAATTTATCATTAAAAATGG+TGG + chr4.4:31215971-31215990 MS.gene031581:CDS 20.0%
!! TAAGATTAAAGGATTTGAAA+AGG + chr4.4:31216069-31216088 MS.gene031581:CDS 20.0%
!! TAGCAAAAAACAATACATAT+TGG + chr4.4:31214960-31214979 MS.gene031581:CDS 20.0%
!! TTTGACTATTGAAGAATTTA+AGG + chr4.4:31215221-31215240 MS.gene031581:CDS 20.0%
!!! CATTTTCAAAACAAAATCAA+CGG - chr4.4:31215787-31215806 None:intergenic 20.0%
!!! GTTTTGAAAATGAAACAAAA+GGG + chr4.4:31215795-31215814 MS.gene031581:intron 20.0%
!!! TGTTTTGAAAATGAAACAAA+AGG + chr4.4:31215794-31215813 MS.gene031581:intron 20.0%
! ATGCACAATAGTTAATAAGA+AGG - chr4.4:31215629-31215648 None:intergenic 25.0%
! TTTCATATAATAAAGCGGTT+CGG - chr4.4:31215573-31215592 None:intergenic 25.0%
!!! AGTTTCATGTTTTATTCTAG+TGG + chr4.4:31216172-31216191 MS.gene031581:CDS 25.0%
!!! TATGTATTGTTTTTTGCTAG+TGG - chr4.4:31214959-31214978 None:intergenic 25.0%
!!! TTAGTTTTCCTGTAGTTATT+TGG - chr4.4:31215865-31215884 None:intergenic 25.0%
AACACGTTTCCAAATATTCA+AGG + chr4.4:31215122-31215141 MS.gene031581:CDS 30.0%
AATCTTCTTGTAACCATTAC+GGG - chr4.4:31215250-31215269 None:intergenic 30.0%
ATTGGATTGTGAAGAATTCA+TGG + chr4.4:31216275-31216294 MS.gene031581:CDS 30.0%
CAACATTGTCCTTGAATATT+TGG - chr4.4:31215134-31215153 None:intergenic 30.0%
CAATCTTCTTGTAACCATTA+CGG - chr4.4:31215251-31215270 None:intergenic 30.0%
GACTAATTACCCTTATAAAG+CGG + chr4.4:31216006-31216025 MS.gene031581:CDS 30.0%
TATTCTAGTGGAATTTACAC+AGG + chr4.4:31216184-31216203 MS.gene031581:CDS 30.0%
TTCATATAATAAAGCGGTTC+GGG - chr4.4:31215572-31215591 None:intergenic 30.0%
TTGGATTGTGAAGAATTCAT+GGG + chr4.4:31216276-31216295 MS.gene031581:CDS 30.0%
!! TGATTAACACTTAGCTTGTA+AGG - chr4.4:31215192-31215211 None:intergenic 30.0%
AATCCTTTAATCTTAGCCAC+AGG - chr4.4:31216064-31216083 None:intergenic 35.0%
ACTTGAATCACTAGCATCAA+TGG - chr4.4:31216155-31216174 None:intergenic 35.0%
AGTTGGATAAGAAGAATCCA+TGG - chr4.4:31216377-31216396 None:intergenic 35.0%
ATCCTTTAATCTTAGCCACA+GGG - chr4.4:31216063-31216082 None:intergenic 35.0%
CAATGGATACTGATATTGGT+TGG - chr4.4:31216138-31216157 None:intergenic 35.0%
CTTCAATAGTCAAATCAGCA+AGG - chr4.4:31215215-31215234 None:intergenic 35.0%
GAACAATGGATGGTAGAATA+TGG + chr4.4:31215069-31215088 MS.gene031581:CDS 35.0%
GCATCAATGGATACTGATAT+TGG - chr4.4:31216142-31216161 None:intergenic 35.0%
GGTATCAACCAAATAACTAC+AGG + chr4.4:31215854-31215873 MS.gene031581:CDS 35.0%
TACAAGAAAGACACGAACAA+TGG + chr4.4:31215055-31215074 MS.gene031581:CDS 35.0%
TCGTGTCTTTCTTGTAAAGA+TGG - chr4.4:31215051-31215070 None:intergenic 35.0%
TGGATTGTGAAGAATTCATG+GGG + chr4.4:31216277-31216296 MS.gene031581:CDS 35.0%
TTCAAGTCAGTGATCAAACT+CGG + chr4.4:31215513-31215532 MS.gene031581:intron 35.0%
! ACCATTTCTCTAGAAACTAG+AGG - chr4.4:31215450-31215469 None:intergenic 35.0%
! ATGGTGACTCATACAGTTTT+CGG - chr4.4:31215032-31215051 None:intergenic 35.0%
! ATGGTGCCTAATAATTTTCG+TGG + chr4.4:31215465-31215484 MS.gene031581:intron 35.0%
! TCCTCTAGTTTCTAGAGAAA+TGG + chr4.4:31215446-31215465 MS.gene031581:intron 35.0%
ACATCCTTGATCTTCACCTT+TGG - chr4.4:31215924-31215943 None:intergenic 40.0%
AGAAAGACACGAACAATGGA+TGG + chr4.4:31215059-31215078 MS.gene031581:CDS 40.0%
AGCACTTTCTCGCTATTAAC+TGG - chr4.4:31216097-31216116 None:intergenic 40.0%
CTAGTTGATTGTGACACCAA+AGG + chr4.4:31215905-31215924 MS.gene031581:CDS 40.0%
GCTGTAGATTGGAGGTTAAA+AGG + chr4.4:31215327-31215346 MS.gene031581:CDS 40.0%
GGAATTTACACAGGAGAATG+TGG + chr4.4:31216193-31216212 MS.gene031581:CDS 40.0%
TCAAGTCAGTGATCAAACTC+GGG + chr4.4:31215514-31215533 MS.gene031581:intron 40.0%
TCCTTTAATCTTAGCCACAG+GGG - chr4.4:31216062-31216081 None:intergenic 40.0%
TTATCTTACCACATTGACCC+TGG - chr4.4:31215374-31215393 None:intergenic 40.0%
! AGAGGTATAGCTACTAAGGA+AGG + chr4.4:31216337-31216356 MS.gene031581:CDS 40.0%
! GAATTTAAGGCTTCCCGTAA+TGG + chr4.4:31215234-31215253 MS.gene031581:CDS 40.0%
! GCAAAGAGGTATAGCTACTA+AGG + chr4.4:31216333-31216352 MS.gene031581:CDS 40.0%
! GTTGGTTATGGTAGTGCTAA+TGG + chr4.4:31216244-31216263 MS.gene031581:CDS 40.0%
! TGGTGACTCATACAGTTTTC+GGG - chr4.4:31215031-31215050 None:intergenic 40.0%
! TTCCATCAGCCGCTTTATAA+GGG - chr4.4:31216018-31216037 None:intergenic 40.0%
!! AACTAGCTCTTGTTCTGAGA+GGG - chr4.4:31215891-31215910 None:intergenic 40.0%
!! GTGCTAATGGCACTGATTAT+TGG + chr4.4:31216257-31216276 MS.gene031581:CDS 40.0%
AACCAACAGCAGTAACACCA+TGG - chr4.4:31216231-31216250 None:intergenic 45.0%
ACCCCTGTGGCTAAGATTAA+AGG + chr4.4:31216058-31216077 MS.gene031581:CDS 45.0%
AGGATGTGAAGGAGGACTAA+TGG + chr4.4:31215937-31215956 MS.gene031581:CDS 45.0%
AGGCTTATGTGGTATTGCCA+TGG + chr4.4:31216357-31216376 MS.gene031581:CDS 45.0%
ATGAAACAAAAGGGAGCTGC+TGG + chr4.4:31215804-31215823 MS.gene031581:intron 45.0%
CTATTCCAGCAGCTGTAGAT+TGG + chr4.4:31215316-31215335 MS.gene031581:CDS 45.0%
GAAGATCAAGGATGTGAAGG+AGG + chr4.4:31215929-31215948 MS.gene031581:CDS 45.0%
GAAGGAGGACTAATGGAAGA+TGG + chr4.4:31215944-31215963 MS.gene031581:CDS 45.0%
GACACCAAAGGTGAAGATCA+AGG + chr4.4:31215917-31215936 MS.gene031581:CDS 45.0%
GCTACTAAGGAAGGCTTATG+TGG + chr4.4:31216346-31216365 MS.gene031581:CDS 45.0%
GGGTACATAAGGATGCAAAG+AGG + chr4.4:31216319-31216338 MS.gene031581:CDS 45.0%
GGTGAAGATCAAGGATGTGA+AGG + chr4.4:31215926-31215945 MS.gene031581:CDS 45.0%
GTATGAAGATGCTGCTGAGA+AGG + chr4.4:31215098-31215117 MS.gene031581:CDS 45.0%
TACCCTTATAAAGCGGCTGA+TGG + chr4.4:31216013-31216032 MS.gene031581:CDS 45.0%
TGAAACAAAAGGGAGCTGCT+GGG + chr4.4:31215805-31215824 MS.gene031581:intron 45.0%
TGGTAATACCAGCAAGTGCA+AGG - chr4.4:31214999-31215018 None:intergenic 45.0%
TTTAATCTTAGCCACAGGGG+TGG - chr4.4:31216059-31216078 None:intergenic 45.0%
! CAACTAGCTCTTGTTCTGAG+AGG - chr4.4:31215892-31215911 None:intergenic 45.0%
! CTTCCATCAGCCGCTTTATA+AGG - chr4.4:31216019-31216038 None:intergenic 45.0%
!! CAGTTTTCGGGACATGACAT+TGG - chr4.4:31215019-31215038 None:intergenic 45.0%
!! GAAAGTGCTTCTCAAAGCTG+TGG + chr4.4:31216108-31216127 MS.gene031581:CDS 45.0%
!! GGTGTTACTGCTGTTGGTTA+TGG + chr4.4:31216232-31216251 MS.gene031581:CDS 45.0%
AACCTCCAATCTACAGCTGC+TGG - chr4.4:31215324-31215343 None:intergenic 50.0%
AATTCATGGGGCACAGTGTG+GGG + chr4.4:31216289-31216308 MS.gene031581:CDS 50.0%
AGAATTCATGGGGCACAGTG+TGG + chr4.4:31216287-31216306 MS.gene031581:CDS 50.0%
AGGAGCTGTCACTCCAATCA+AGG + chr4.4:31215347-31215366 MS.gene031581:CDS 50.0%
AGTCAGTGATCAAACTCGGG+TGG + chr4.4:31215517-31215536 MS.gene031581:intron 50.0%
ATCAAGGACCAGGGTCAATG+TGG + chr4.4:31215363-31215382 MS.gene031581:CDS 50.0%
CATTGACCCTGGTCCTTGAT+TGG - chr4.4:31215363-31215382 None:intergenic 50.0%
GAATTCATGGGGCACAGTGT+GGG + chr4.4:31216288-31216307 MS.gene031581:CDS 50.0%
GTCACTCCAATCAAGGACCA+GGG + chr4.4:31215354-31215373 MS.gene031581:CDS 50.0%
TACCAGCAAGTGCAAGGATG+AGG - chr4.4:31214993-31215012 None:intergenic 50.0%
TCAACAGTGGCTGCAACTGA+GGG + chr4.4:31215833-31215852 MS.gene031581:CDS 50.0%
TGTCACTCCAATCAAGGACC+AGG + chr4.4:31215353-31215372 MS.gene031581:CDS 50.0%
TGTGGAACTGAGCTAGACCA+TGG + chr4.4:31216211-31216230 MS.gene031581:CDS 50.0%
TTCAACAGTGGCTGCAACTG+AGG + chr4.4:31215832-31215851 MS.gene031581:CDS 50.0%
TTCCAGCAGCTGTAGATTGG+AGG + chr4.4:31215319-31215338 MS.gene031581:CDS 50.0%
TTCCTCATCCTTGCACTTGC+TGG + chr4.4:31214988-31215007 MS.gene031581:CDS 50.0%
! CTGCTGGGCATTTTCAACAG+TGG + chr4.4:31215820-31215839 MS.gene031581:CDS 50.0%
! GGGGTGAGAAAGGGTACATA+AGG + chr4.4:31216308-31216327 MS.gene031581:CDS 50.0%
!! GACCATGGTGTTACTGCTGT+TGG + chr4.4:31216226-31216245 MS.gene031581:CDS 50.0%
AATCTTAGCCACAGGGGTGG+TGG - chr4.4:31216056-31216075 None:intergenic 55.0%
GCACAGTGTGGGGTGAGAAA+GGG + chr4.4:31216299-31216318 MS.gene031581:CDS 55.0%
GGCACAGTGTGGGGTGAGAA+AGG + chr4.4:31216298-31216317 MS.gene031581:CDS 60.0%
CACTGCAACCACCACCCCTG+TGG + chr4.4:31216045-31216064 MS.gene031581:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 31214949 31216402 31214949 ID=MS.gene031581
chr4.4 mRNA 31214949 31216402 31214949 ID=MS.gene031581.t1;Parent=MS.gene031581
chr4.4 exon 31214949 31215384 31214949 ID=MS.gene031581.t1.exon1;Parent=MS.gene031581.t1
chr4.4 CDS 31214949 31215384 31214949 ID=cds.MS.gene031581.t1;Parent=MS.gene031581.t1
chr4.4 exon 31215816 31216402 31215816 ID=MS.gene031581.t1.exon2;Parent=MS.gene031581.t1
chr4.4 CDS 31215816 31216402 31215816 ID=cds.MS.gene031581.t1;Parent=MS.gene031581.t1
Gene Sequence

>MS.gene031581

ATGGTTTCCACTAGCAAAAAACAATACATATTGGCTTTGTTCCTCATCCTTGCACTTGCTGGTATTACCAATGTCATGTCCCGAAAACTGTATGAGTCACCATCTTTACAAGAAAGACACGAACAATGGATGGTAGAATATGGCAAAGTGTATGAAGATGCTGCTGAGAAGGAAACACGTTTCCAAATATTCAAGGACAATGTTGAGTTCATTGAATCATTCAATGCTGCTGACAACAAACCTTACAAGCTAAGTGTTAATCACCTTGCTGATTTGACTATTGAAGAATTTAAGGCTTCCCGTAATGGTTACAAGAAGATTGCTCGTGAATTTACTACGACATCGTTTAAGTATGAAAATGTTACTGCTATTCCAGCAGCTGTAGATTGGAGGTTAAAAGGAGCTGTCACTCCAATCAAGGACCAGGGTCAATGTGGTAAGATAAACAATATCATACATAAAAAAAATACTTTATTATATGATTTTCTTCTTCTTAATCCTCTAGTTTCTAGAGAAATGGTGCCTAATAATTTTCGTGGATAAATAAAGTCTGATCAAAGATTATTCAAGTCAGTGATCAAACTCGGGTGGTCTTAATCGCTTCAACTTTAACAATTTGACCCGAACCGCTTTATTATATGAAATTAATTACACAAATGTTCAAACTTCAATCATATCCTTCTTATTAACTATTGTGCATAAATTAATAAACTTTTTTCCTCAAAAAAAAATTAATAAACTTTTGTTATATATGGTATAGTATAGTGTTCTTACTATGATTTTATTTGATGTGATTATATTGAGTCTAATTAATTGGATTAAATAAATAATATTTCCGTTGATTTTGTTTTGAAAATGAAACAAAAGGGAGCTGCTGGGCATTTTCAACAGTGGCTGCAACTGAGGGTATCAACCAAATAACTACAGGAAAACTAATCTCCCTCTCAGAACAAGAGCTAGTTGATTGTGACACCAAAGGTGAAGATCAAGGATGTGAAGGAGGACTAATGGAAGATGGATTTGAATTTATCATTAAAAATGGTGGAATCACAAGTGAGACTAATTACCCTTATAAAGCGGCTGATGGAAGCTGTAACACTGCAACCACCACCCCTGTGGCTAAGATTAAAGGATTTGAAAAGGTTCCAGTTAATAGCGAGAAAGTGCTTCTCAAAGCTGTGGCGAACCAACCAATATCAGTATCCATTGATGCTAGTGATTCAAGTTTCATGTTTTATTCTAGTGGAATTTACACAGGAGAATGTGGAACTGAGCTAGACCATGGTGTTACTGCTGTTGGTTATGGTAGTGCTAATGGCACTGATTATTGGATTGTGAAGAATTCATGGGGCACAGTGTGGGGTGAGAAAGGGTACATAAGGATGCAAAGAGGTATAGCTACTAAGGAAGGCTTATGTGGTATTGCCATGGATTCTTCTTATCCAACTGCTTAA
Protein sequence

>MS.gene031581.t1

MVSTSKKQYILALFLILALAGITNVMSRKLYESPSLQERHEQWMVEYGKVYEDAAEKETRFQIFKDNVEFIESFNAADNKPYKLSVNHLADLTIEEFKASRNGYKKIAREFTTTSFKYENVTAIPAAVDWRLKGAVTPIKDQGQCGSCWAFSTVAATEGINQITTGKLISLSEQELVDCDTKGEDQGCEGGLMEDGFEFIIKNGGITSETNYPYKAADGSCNTATTTPVAKIKGFEKVPVNSEKVLLKAVANQPISVSIDASDSSFMFYSSGIYTGECGTELDHGVTAVGYGSANGTDYWIVKNSWGTVWGEKGYIRMQRGIATKEGLCGIAMDSSYPTA