AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031619

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031619.t1 MTR_4g079770 90.145 345 30 3 1 341 1 345 0.0 649
MS.gene031619.t1 MTR_5g022560 86.919 344 42 2 1 341 1 344 0.0 631
MS.gene031619.t1 MTR_4g079470 90.432 324 28 2 21 341 22 345 0.0 615
MS.gene031619.t1 MTR_4g079800 89.815 324 30 2 21 341 22 345 0.0 613
MS.gene031619.t1 MTR_4g107930 85.217 345 47 3 1 341 1 345 0.0 610
MS.gene031619.t1 MTR_4g047610 86.919 344 41 3 1 341 1 343 0.0 603
MS.gene031619.t1 MTR_2g075830 76.093 343 78 3 1 341 1 341 0.0 533
MS.gene031619.t1 MTR_4g080730 61.095 347 122 7 1 341 1 340 2.53e-149 424
MS.gene031619.t1 MTR_4g080700 64.087 323 103 7 25 341 25 340 2.67e-149 424
MS.gene031619.t1 MTR_4g104370 57.478 341 135 9 6 339 5 342 2.73e-134 387
MS.gene031619.t1 MTR_3g075390 53.235 340 144 7 14 340 7 344 1.26e-129 375
MS.gene031619.t1 MTR_4g080360 53.731 335 149 5 12 341 9 342 1.29e-128 372
MS.gene031619.t1 MTR_2g083930 54.464 336 139 5 12 339 13 342 2.96e-128 370
MS.gene031619.t1 MTR_4g079440 54.870 308 134 3 33 339 40 343 3.45e-122 355
MS.gene031619.t1 MTR_3g116080 51.032 339 152 6 11 340 14 347 7.90e-120 349
MS.gene031619.t1 MTR_4g094918 51.032 339 155 6 10 340 6 341 6.24e-119 347
MS.gene031619.t1 MTR_2g090890 50.148 337 157 4 10 340 5 336 1.95e-118 345
MS.gene031619.t1 MTR_1g107550 55.627 311 130 5 35 339 46 354 3.59e-118 350
MS.gene031619.t1 MTR_7g078600 56.508 315 122 8 31 339 31 336 2.09e-117 343
MS.gene031619.t1 MTR_7g079230 51.064 329 149 7 16 339 13 334 1.01e-112 331
MS.gene031619.t1 MTR_3g102220 52.000 325 139 7 27 339 41 360 1.43e-112 335
MS.gene031619.t1 MTR_1g018840 52.733 311 132 5 39 339 55 360 1.81e-112 335
MS.gene031619.t1 MTR_1g018840 52.733 311 132 5 39 339 55 360 3.25e-112 334
MS.gene031619.t1 MTR_3g112430 50.323 310 140 5 40 340 37 341 3.17e-100 303
MS.gene031619.t1 MTR_2g015850 52.921 291 129 6 53 339 76 362 1.10e-97 293
MS.gene031619.t1 MTR_2g016180 45.087 346 166 11 11 340 18 355 2.11e-88 274
MS.gene031619.t1 MTR_2g016270 45.029 342 157 11 11 340 18 340 5.05e-87 270
MS.gene031619.t1 MTR_2g067900 44.304 316 154 11 38 338 15 323 4.09e-74 232
MS.gene031619.t1 MTR_4g125300 41.722 302 162 7 45 341 57 349 3.88e-72 228
MS.gene031619.t1 MTR_2g067560 44.375 320 146 13 38 335 15 324 3.99e-71 238
MS.gene031619.t1 MTR_2g015920 38.941 321 174 11 33 340 47 358 6.33e-69 220
MS.gene031619.t1 MTR_2g064475 40.115 349 151 16 38 340 15 351 9.15e-69 219
MS.gene031619.t1 MTR_2g066980 35.574 357 168 13 7 340 1 318 6.52e-65 208
MS.gene031619.t1 MTR_7g078630 44.366 284 119 5 28 300 695 950 1.49e-64 220
MS.gene031619.t1 MTR_4g065077 38.509 322 176 9 32 340 44 356 3.47e-64 207
MS.gene031619.t1 MTR_4g071060 36.944 360 187 12 9 337 3 353 6.12e-64 207
MS.gene031619.t1 MTR_1g023810 44.156 231 120 6 111 338 37 261 5.64e-63 201
MS.gene031619.t1 MTR_2g015930 38.871 319 170 11 37 340 50 358 1.36e-61 201
MS.gene031619.t1 MTR_1g023210 36.607 336 171 10 23 332 33 352 3.59e-61 200
MS.gene031619.t1 MTR_8g086470 37.421 318 169 9 32 340 60 356 1.36e-59 196
MS.gene031619.t1 MTR_1g094135 35.376 359 190 14 7 337 19 363 2.54e-53 180
MS.gene031619.t1 MTR_4g072790 33.333 294 161 7 47 337 56 317 7.04e-42 148
MS.gene031619.t1 MTR_7g078580 64.286 98 34 1 242 339 4 100 1.36e-41 141
MS.gene031619.t1 MTR_4g073660 34.448 299 155 9 47 337 55 320 5.16e-41 146
MS.gene031619.t1 MTR_4g072340 30.986 355 193 11 1 337 1 321 6.12e-41 146
MS.gene031619.t1 MTR_7g079130 70.000 90 27 0 250 339 7 96 1.12e-40 138
MS.gene031619.t1 MTR_2g015840 46.154 156 80 3 7 158 1 156 2.82e-39 140
MS.gene031619.t1 MTR_2g015840 40.704 199 98 6 160 340 75 271 2.05e-37 135
MS.gene031619.t1 MTR_1g025450 37.433 187 92 7 111 293 43 208 6.49e-33 128
MS.gene031619.t1 MTR_2g016380 46.281 121 58 4 224 340 18 135 1.30e-26 106
MS.gene031619.t1 MTR_7g078310 44.286 140 68 3 80 216 115 247 1.94e-26 105
MS.gene031619.t1 MTR_7g111060 26.518 313 182 13 55 332 31 330 3.65e-24 101
MS.gene031619.t1 MTR_3g029560 30.159 252 139 9 96 340 16 237 1.18e-22 95.1
MS.gene031619.t1 MTR_7g111050 26.250 320 194 13 52 340 29 337 1.29e-22 97.4
MS.gene031619.t1 MTR_7g079260 59.722 72 13 1 266 337 7 62 3.37e-22 90.1
MS.gene031619.t1 MTR_8g087510 40.458 131 71 5 164 292 23 148 8.34e-19 83.2
MS.gene031619.t1 MTR_2g047960 47.059 85 44 1 132 216 7 90 4.10e-18 79.0
MS.gene031619.t1 MTR_3g082370 26.442 208 133 5 127 319 4 206 1.63e-16 78.2
MS.gene031619.t1 MTR_7g111050 24.286 280 176 11 52 302 24 296 1.95e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031619.t1 AT5G45890 54.783 345 143 7 7 340 3 345 2.38e-134 386
MS.gene031619.t1 AT5G50260 53.644 343 136 6 14 339 5 341 1.49e-131 380
MS.gene031619.t1 AT3G48340 54.203 345 135 8 12 339 4 342 3.68e-126 366
MS.gene031619.t1 AT2G34080 50.733 341 156 6 10 341 8 345 3.93e-121 353
MS.gene031619.t1 AT4G35350 50.581 344 155 7 7 340 14 352 6.43e-118 345
MS.gene031619.t1 AT3G48350 54.603 315 135 6 32 340 31 343 1.85e-116 342
MS.gene031619.t1 AT3G49340 49.855 345 147 8 16 341 4 341 2.28e-116 340
MS.gene031619.t1 AT3G19390 51.057 331 157 5 13 340 17 345 6.53e-116 343
MS.gene031619.t1 AT5G43060 53.086 324 139 6 24 339 34 352 1.09e-115 343
MS.gene031619.t1 AT1G06260 50.733 341 150 6 9 339 9 341 6.91e-115 337
MS.gene031619.t1 AT2G27420 49.568 347 157 6 10 341 5 348 6.53e-114 334
MS.gene031619.t1 AT1G47128 52.443 307 135 6 39 339 50 351 3.13e-112 334
MS.gene031619.t1 AT3G19400 50.654 306 146 5 39 340 44 348 1.72e-110 326
MS.gene031619.t1 AT1G29090 46.875 352 170 7 4 341 7 355 2.39e-110 325
MS.gene031619.t1 AT1G20850 50.637 314 144 5 33 340 45 353 2.90e-110 325
MS.gene031619.t1 AT4G36880 50.769 325 139 7 32 340 42 361 3.54e-109 323
MS.gene031619.t1 AT1G29080 47.147 333 153 7 24 341 22 346 2.22e-105 313
MS.gene031619.t1 AT1G09850 49.515 309 152 4 33 339 26 332 4.19e-100 302
MS.gene031619.t1 AT4G23520 47.557 307 148 8 40 339 48 348 1.13e-99 298
MS.gene031619.t1 AT4G11310 46.602 309 149 6 40 339 50 351 6.98e-99 296
MS.gene031619.t1 AT4G11320 45.367 313 147 6 40 339 57 358 1.56e-97 293
MS.gene031619.t1 AT4G11320 45.367 313 147 6 40 339 57 358 1.56e-97 293
MS.gene031619.t1 AT1G29110 42.508 327 152 7 29 340 28 333 1.63e-86 264
MS.gene031619.t1 AT3G43960 42.900 331 179 8 16 339 18 345 1.23e-85 263
MS.gene031619.t1 AT4G35350 47.482 278 131 7 7 274 14 286 3.18e-83 254
MS.gene031619.t1 AT3G48350 51.406 249 113 6 32 274 31 277 1.85e-77 239
MS.gene031619.t1 AT5G60360 42.484 306 162 8 41 341 61 357 8.19e-77 240
MS.gene031619.t1 AT3G19400 48.750 240 119 4 39 274 44 283 1.52e-76 237
MS.gene031619.t1 AT5G60360 42.157 306 162 9 41 341 61 356 3.89e-74 233
MS.gene031619.t1 AT3G45310 41.424 309 161 10 41 341 61 357 1.93e-73 231
MS.gene031619.t1 AT5G60360 41.892 296 158 8 41 331 61 347 2.68e-72 228
MS.gene031619.t1 AT3G45310 41.100 309 161 11 41 341 61 356 7.93e-71 224
MS.gene031619.t1 AT2G21430 37.874 301 165 7 47 332 56 349 8.91e-61 199
MS.gene031619.t1 AT4G39090 38.387 310 161 10 47 337 59 357 2.30e-59 195
MS.gene031619.t1 AT3G54940 37.736 318 162 9 38 332 50 354 2.34e-57 190
MS.gene031619.t1 AT4G16190 36.893 309 167 8 48 337 64 363 1.97e-55 185
MS.gene031619.t1 AT3G54940 37.662 231 128 4 38 256 50 276 2.09e-41 146
MS.gene031619.t1 AT1G02305 28.283 297 162 12 71 332 53 333 2.96e-26 107
MS.gene031619.t1 AT4G01610 26.688 311 188 12 51 332 31 330 1.33e-23 100
MS.gene031619.t1 AT4G01610 26.367 311 189 12 51 332 31 330 2.43e-22 96.7
MS.gene031619.t1 AT1G02300 28.638 213 122 6 142 332 146 350 2.36e-20 91.3
MS.gene031619.t1 AT2G22160 40.659 91 53 1 53 142 8 98 2.48e-17 77.0

Find 86 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCCTGCTGCTACCATTAC+TGG 0.241381 4.3:-29340166 MS.gene031619:CDS
AATATGATATAATTGATTAT+TGG 0.245014 4.3:+29340940 None:intergenic
TAAGTTAAGTACTGGAAAAT+TGG 0.257813 4.3:-29340365 MS.gene031619:CDS
GCATCAATGGCTACAGAAAT+TGG 0.307524 4.3:+29340085 None:intergenic
ACAATGATGGAACCAAATAT+TGG 0.314081 4.3:-29339964 MS.gene031619:CDS
TCACCATGGCAAAGTGTATA+AGG 0.340501 4.3:-29340816 MS.gene031619:CDS
TGTTCCGTCCACACCTTGAT+AGG 0.347233 4.3:+29340210 None:intergenic
GTATGGCTTGTTAGCATTGT+TGG 0.355877 4.3:+29340730 None:intergenic
TTGCAGTGCGTTCTCATTGT+TGG 0.372109 4.3:+29340126 None:intergenic
AGGTTGTGAGGGTGGTCTTA+TGG 0.376813 4.3:-29340290 MS.gene031619:CDS
GGTGTTACTGCTGTGGGATA+TGG 0.378598 4.3:-29339992 MS.gene031619:CDS
TGATTAATGCCAAGCTTGTA+TGG 0.387555 4.3:+29340713 None:intergenic
TCAATTATATCATATTTCAT+TGG 0.405472 4.3:-29340933 MS.gene031619:CDS
AAATTCATCAATCAAAATCA+TGG 0.416498 4.3:-29340256 MS.gene031619:CDS
GGAACATGCAATGCTAATAA+AGG 0.418975 4.3:-29340193 MS.gene031619:CDS
ACTTTGTGGCATTGCAATGC+AGG 0.423561 4.3:-29339861 MS.gene031619:CDS
TGCCTTTATAGGATGTTGTT+GGG 0.430973 4.3:-29340422 MS.gene031619:intron
CACACCTTGATAGGGGTATT+CGG 0.435671 4.3:+29340219 None:intergenic
ATGCCTTTATAGGATGTTGT+TGG 0.435997 4.3:-29340423 MS.gene031619:intron
AAAACTCATGGGGAACCGAC+TGG 0.445030 4.3:-29339934 MS.gene031619:CDS
TACAAAAGTGGTGTCTTCAC+TGG 0.452446 4.3:-29340040 MS.gene031619:CDS
AGTGCGTTCTCATTGTTGGC+AGG 0.452809 4.3:+29340130 None:intergenic
GGGATTCATAAGTTAAGTAC+TGG 0.452855 4.3:-29340373 MS.gene031619:CDS
ACAATCAACAAGTTCTTGTT+CGG 0.454613 4.3:+29340333 None:intergenic
GGATGATATGAACTCCTCAT+TGG 0.466065 4.3:+29340676 None:intergenic
GTCGGATCCACTAGCATCAA+TGG 0.467753 4.3:+29340072 None:intergenic
TGCTCGTGTTTCTCATACAT+AGG 0.475196 4.3:+29340848 None:intergenic
TCAGCTATTGCGGCAACTGA+AGG 0.488495 4.3:-29340394 MS.gene031619:CDS
TTCCTCCAATCCATTGTGGA+TGG 0.490858 4.3:+29340578 None:intergenic
TGCTACCATTACTGGGTATG+AGG 0.491803 4.3:-29340158 MS.gene031619:CDS
CTCCTGCTGCTACCATTACT+GGG 0.496600 4.3:-29340165 MS.gene031619:CDS
ATATTTAAAAGTAGTTGTCC+TGG 0.500162 4.3:+29340616 None:intergenic
AAACTCATGGGGAACCGACT+GGG 0.502307 4.3:-29339933 MS.gene031619:CDS
ATGGGGAACCGACTGGGGTG+AGG 0.510047 4.3:-29339927 MS.gene031619:CDS
TTTCTTCCTCCAATCCATTG+TGG 0.510059 4.3:+29340574 None:intergenic
GTTCCGTCCACACCTTGATA+GGG 0.517808 4.3:+29340211 None:intergenic
ACAATGGATTGGAGGAAGAA+AGG 0.520772 4.3:-29340572 MS.gene031619:CDS
GGGGTGAGGAAGGATACATT+AGG 0.521721 4.3:-29339913 MS.gene031619:CDS
AGGATACATTAGGATGCAAA+GGG 0.522408 4.3:-29339903 MS.gene031619:CDS
CAGCTATTGCGGCAACTGAA+GGG 0.522776 4.3:-29340393 MS.gene031619:CDS
GGATATGGCATTAACAATGA+TGG 0.525852 4.3:-29339977 MS.gene031619:CDS
TCTGTAGCCATTGATGCTAG+TGG 0.529031 4.3:-29340079 MS.gene031619:CDS
TAATTGATTATTGGCAGCCA+TGG 0.531448 4.3:+29340949 None:intergenic
ATGTTTGACATTTAAGCCGT+AGG 0.532128 4.3:+29339830 None:intergenic
CTTGTTGATTGTGACACAAA+AGG 0.533495 4.3:-29340322 MS.gene031619:CDS
GCTCGTGTTTCTCATACATA+GGG 0.536434 4.3:+29340849 None:intergenic
GGAACCGACTGGGGTGAGGA+AGG 0.537425 4.3:-29339923 MS.gene031619:CDS
GCTAACAAGCCATACAAGCT+TGG 0.545790 4.3:-29340722 MS.gene031619:CDS
TGTATGAGAAACACGAGCAA+TGG 0.547122 4.3:-29340844 MS.gene031619:CDS
TGTGGTACTGAGTTGGATCA+TGG 0.551365 4.3:-29340013 MS.gene031619:CDS
TTCCATCCACAATGGATTGG+AGG 0.551446 4.3:-29340580 MS.gene031619:CDS
GTGTTCCATCCACAATGGAT+TGG 0.552593 4.3:-29340583 MS.gene031619:CDS
TGATGGAACCAAATATTGGT+TGG 0.554328 4.3:-29339960 MS.gene031619:CDS
TACCCAGTAATGGTAGCAGC+AGG 0.558279 4.3:+29340163 None:intergenic
GTGCGTTCTCATTGTTGGCA+GGG 0.560091 4.3:+29340131 None:intergenic
AAGGATACATTAGGATGCAA+AGG 0.563165 4.3:-29339904 MS.gene031619:CDS
GGATCATGGTGTTACTGCTG+TGG 0.569092 4.3:-29339999 MS.gene031619:CDS
GATGCTGCAGAAGGACTTTG+TGG 0.573462 4.3:-29339875 MS.gene031619:CDS
AATGCAGGCATCTTATCCTA+CGG 0.576356 4.3:-29339846 MS.gene031619:CDS
TACCCCTATCAAGGTGTGGA+CGG 0.577316 4.3:-29340214 MS.gene031619:CDS
AACTCATGGGGAACCGACTG+GGG 0.580555 4.3:-29339932 MS.gene031619:CDS
TGGTTCATGTGGTACTGAGT+TGG 0.586183 4.3:-29340020 MS.gene031619:CDS
GATCATGGTGTTACTGCTGT+GGG 0.587872 4.3:-29339998 MS.gene031619:CDS
GGTGTAGACCAAGGTTGTGA+GGG 0.591635 4.3:-29340301 MS.gene031619:CDS
TAAGACCACCCTCACAACCT+TGG 0.592405 4.3:+29340293 None:intergenic
AGGTGTAGACCAAGGTTGTG+AGG 0.594253 4.3:-29340302 MS.gene031619:CDS
GTAGACCAAGGTTGTGAGGG+TGG 0.595756 4.3:-29340298 MS.gene031619:CDS
TTCCGTCCACACCTTGATAG+GGG 0.599701 4.3:+29340212 None:intergenic
GAGGCCGAATACCCCTATCA+AGG 0.601939 4.3:-29340223 MS.gene031619:CDS
GACATCCTCATACCCAGTAA+TGG 0.602674 4.3:+29340153 None:intergenic
GATCCTTATACACTTTGCCA+TGG 0.603597 4.3:+29340813 None:intergenic
ACATGTGCTCTTCAATCACC+AGG 0.611193 4.3:-29340634 MS.gene031619:CDS
TGAGAAACACGAGCAATGGA+TGG 0.613416 4.3:-29340840 MS.gene031619:CDS
GGTGTCTTCACTGGTTCATG+TGG 0.623688 4.3:-29340031 MS.gene031619:CDS
AGGGGTGTAGATGCTGCAGA+AGG 0.625926 4.3:-29339884 MS.gene031619:CDS
GTCATCTTGTAGAGTGCGAG+AGG 0.637765 4.3:+29340871 None:intergenic
GTATCCTTCCTCACCCCAGT+CGG 0.644090 4.3:+29339919 None:intergenic
ATATGAACTCCTCATTGGTG+AGG 0.644741 4.3:+29340681 None:intergenic
TGCGAGTGTTCCATCCACAA+TGG 0.653708 4.3:-29340588 MS.gene031619:CDS
ATTTGCAGACCTCACCAATG+AGG 0.664553 4.3:-29340690 MS.gene031619:CDS
CGAATACCCCTATCAAGGTG+TGG 0.692068 4.3:-29340218 MS.gene031619:CDS
GAGCAATGGATGGCTCACCA+TGG 0.701771 4.3:-29340830 MS.gene031619:CDS
GACACAAAAGGTGTAGACCA+AGG 0.707755 4.3:-29340310 MS.gene031619:CDS
AAATCATGGAATCAGTACTG+AGG 0.718091 4.3:-29340242 MS.gene031619:CDS
GGATTGGAGGAAGAAAGGAG+CGG 0.765865 4.3:-29340567 MS.gene031619:CDS
GGATACATTAGGATGCAAAG+GGG 0.768557 4.3:-29339902 MS.gene031619:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGATATAATTGATTAT+TGG + chr4.3:29339851-29339870 None:intergenic 10.0%
!! GAATTTCTTATAGATATATA+TGG - chr4.3:29340303-29340322 MS.gene031619:CDS 15.0%
!! TCAATTATATCATATTTCAT+TGG - chr4.3:29339855-29339874 MS.gene031619:CDS 15.0%
!!! AAAGGCATATTTAAAATAAT+TGG + chr4.3:29340353-29340372 None:intergenic 15.0%
!!! TATTTTAAATATGCCTTTAT+AGG - chr4.3:29340355-29340374 MS.gene031619:CDS 15.0%
!! AAATTCATCAATCAAAATCA+TGG - chr4.3:29340532-29340551 MS.gene031619:intron 20.0%
! ATATTTAAAAGTAGTTGTCC+TGG + chr4.3:29340175-29340194 None:intergenic 25.0%
! ATCATCCAAAAATAGATTCA+AGG - chr4.3:29340128-29340147 MS.gene031619:CDS 25.0%
! TCATCCAAAAATAGATTCAA+GGG - chr4.3:29340129-29340148 MS.gene031619:CDS 25.0%
!! ACTTTTGTAAAACTGAAAGT+CGG + chr4.3:29340737-29340756 None:intergenic 25.0%
!! GTTTTTAACCAACCAATATT+TGG + chr4.3:29340839-29340858 None:intergenic 25.0%
!!! TAAGTTAAGTACTGGAAAAT+TGG - chr4.3:29340423-29340442 MS.gene031619:intron 25.0%
ACAATCAACAAGTTCTTGTT+CGG + chr4.3:29340458-29340477 None:intergenic 30.0%
CATCCAAAAATAGATTCAAG+GGG - chr4.3:29340130-29340149 MS.gene031619:CDS 30.0%
! ACAATGATGGAACCAAATAT+TGG - chr4.3:29340824-29340843 MS.gene031619:CDS 30.0%
! ACATTGACCTTGATTTTTCA+CGG + chr4.3:29340253-29340272 None:intergenic 30.0%
! ATTGGTTGGTTAAAAACTCA+TGG - chr4.3:29340842-29340861 MS.gene031619:CDS 30.0%
! GACTTTCAGTTTTACAAAAG+TGG - chr4.3:29340736-29340755 MS.gene031619:CDS 30.0%
! TTGGTTGGTTAAAAACTCAT+GGG - chr4.3:29340843-29340862 MS.gene031619:CDS 30.0%
AAATCATGGAATCAGTACTG+AGG - chr4.3:29340546-29340565 MS.gene031619:CDS 35.0%
AAGGATACATTAGGATGCAA+AGG - chr4.3:29340884-29340903 MS.gene031619:CDS 35.0%
AGGATACATTAGGATGCAAA+GGG - chr4.3:29340885-29340904 MS.gene031619:CDS 35.0%
ATGCCTTTATAGGATGTTGT+TGG - chr4.3:29340365-29340384 MS.gene031619:CDS 35.0%
GGAACATGCAATGCTAATAA+AGG - chr4.3:29340595-29340614 MS.gene031619:CDS 35.0%
GGATATGGCATTAACAATGA+TGG - chr4.3:29340811-29340830 MS.gene031619:CDS 35.0%
GGGATTCATAAGTTAAGTAC+TGG - chr4.3:29340415-29340434 MS.gene031619:intron 35.0%
GTGAAAAATCAAGGTCAATG+TGG - chr4.3:29340252-29340271 MS.gene031619:CDS 35.0%
TGATGGAACCAAATATTGGT+TGG - chr4.3:29340828-29340847 MS.gene031619:CDS 35.0%
TGATTAATGCCAAGCTTGTA+TGG + chr4.3:29340078-29340097 None:intergenic 35.0%
TGCCTTTATAGGATGTTGTT+GGG - chr4.3:29340366-29340385 MS.gene031619:CDS 35.0%
! CATTGACCTTGATTTTTCAC+GGG + chr4.3:29340252-29340271 None:intergenic 35.0%
! CTTGTTGATTGTGACACAAA+AGG - chr4.3:29340466-29340485 MS.gene031619:intron 35.0%
! TGGTTGGTTAAAAACTCATG+GGG - chr4.3:29340844-29340863 MS.gene031619:CDS 35.0%
!! ATTGGCATTGCTTTTATGCT+TGG - chr4.3:29339873-29339892 MS.gene031619:CDS 35.0%
!! TTGGCATTGCTTTTATGCTT+GGG - chr4.3:29339874-29339893 MS.gene031619:CDS 35.0%
AATGCAGGCATCTTATCCTA+CGG - chr4.3:29340942-29340961 MS.gene031619:CDS 40.0%
ATATGAACTCCTCATTGGTG+AGG + chr4.3:29340110-29340129 None:intergenic 40.0%
GATCCTTATACACTTTGCCA+TGG + chr4.3:29339978-29339997 None:intergenic 40.0%
GCATCAATGGCTACAGAAAT+TGG + chr4.3:29340706-29340725 None:intergenic 40.0%
GCTCGTGTTTCTCATACATA+GGG + chr4.3:29339942-29339961 None:intergenic 40.0%
GGATACATTAGGATGCAAAG+GGG - chr4.3:29340886-29340905 MS.gene031619:CDS 40.0%
GGATGATATGAACTCCTCAT+TGG + chr4.3:29340115-29340134 None:intergenic 40.0%
TACAAAAGTGGTGTCTTCAC+TGG - chr4.3:29340748-29340767 MS.gene031619:CDS 40.0%
TCACCATGGCAAAGTGTATA+AGG - chr4.3:29339972-29339991 MS.gene031619:CDS 40.0%
TGCTCGTGTTTCTCATACAT+AGG + chr4.3:29339943-29339962 None:intergenic 40.0%
TGTATGAGAAACACGAGCAA+TGG - chr4.3:29339944-29339963 MS.gene031619:CDS 40.0%
TTTCTTCCTCCAATCCATTG+TGG + chr4.3:29340217-29340236 None:intergenic 40.0%
! GCTTTTATGCTTGGGATTGT+GGG - chr4.3:29339882-29339901 MS.gene031619:CDS 40.0%
! GTGCCCCTTGAATCTATTTT+TGG + chr4.3:29340136-29340155 None:intergenic 40.0%
! TGCTTTTATGCTTGGGATTG+TGG - chr4.3:29339881-29339900 MS.gene031619:CDS 40.0%
!! ACAATGGATTGGAGGAAGAA+AGG - chr4.3:29340216-29340235 MS.gene031619:CDS 40.0%
!! GTATGGCTTGTTAGCATTGT+TGG + chr4.3:29340061-29340080 None:intergenic 40.0%
ACATGTGCTCTTCAATCACC+AGG - chr4.3:29340154-29340173 MS.gene031619:CDS 45.0%
ACTTTGTGGCATTGCAATGC+AGG - chr4.3:29340927-29340946 MS.gene031619:CDS 45.0%
ATTTGCAGACCTCACCAATG+AGG - chr4.3:29340098-29340117 MS.gene031619:CDS 45.0%
CGCCCAACAACATCCTATAA+AGG + chr4.3:29340371-29340390 None:intergenic 45.0%
GACATCCTCATACCCAGTAA+TGG + chr4.3:29340638-29340657 None:intergenic 45.0%
GCTAACAAGCCATACAAGCT+TGG - chr4.3:29340066-29340085 MS.gene031619:CDS 45.0%
GTGACACCCGTGAAAAATCA+AGG - chr4.3:29340243-29340262 MS.gene031619:CDS 45.0%
GTGTTCCATCCACAATGGAT+TGG - chr4.3:29340205-29340224 MS.gene031619:CDS 45.0%
TCTGTAGCCATTGATGCTAG+TGG - chr4.3:29340709-29340728 MS.gene031619:CDS 45.0%
TGAGAAACACGAGCAATGGA+TGG - chr4.3:29339948-29339967 MS.gene031619:CDS 45.0%
TGCTACCATTACTGGGTATG+AGG - chr4.3:29340630-29340649 MS.gene031619:CDS 45.0%
TTCCTCCAATCCATTGTGGA+TGG + chr4.3:29340213-29340232 None:intergenic 45.0%
TTGCAGTGCGTTCTCATTGT+TGG + chr4.3:29340665-29340684 None:intergenic 45.0%
! GACACAAAAGGTGTAGACCA+AGG - chr4.3:29340478-29340497 MS.gene031619:intron 45.0%
! TGGTTCATGTGGTACTGAGT+TGG - chr4.3:29340768-29340787 MS.gene031619:CDS 45.0%
! TGTGGTACTGAGTTGGATCA+TGG - chr4.3:29340775-29340794 MS.gene031619:CDS 45.0%
! TTGGGCGTTTTCAGCTATTG+CGG - chr4.3:29340384-29340403 MS.gene031619:CDS 45.0%
!! CACACCTTGATAGGGGTATT+CGG + chr4.3:29340572-29340591 None:intergenic 45.0%
!! GATCATGGTGTTACTGCTGT+GGG - chr4.3:29340790-29340809 MS.gene031619:CDS 45.0%
!! TTCCATCCACAATGGATTGG+AGG - chr4.3:29340208-29340227 MS.gene031619:CDS 45.0%
AAAACTCATGGGGAACCGAC+TGG - chr4.3:29340854-29340873 MS.gene031619:CDS 50.0%
AAACTCATGGGGAACCGACT+GGG - chr4.3:29340855-29340874 MS.gene031619:CDS 50.0%
AGGTGTAGACCAAGGTTGTG+AGG - chr4.3:29340486-29340505 MS.gene031619:intron 50.0%
AGTGCGTTCTCATTGTTGGC+AGG + chr4.3:29340661-29340680 None:intergenic 50.0%
CAGCTATTGCGGCAACTGAA+GGG - chr4.3:29340395-29340414 MS.gene031619:CDS 50.0%
CGAATACCCCTATCAAGGTG+TGG - chr4.3:29340570-29340589 MS.gene031619:CDS 50.0%
CTCCTGCTGCTACCATTACT+GGG - chr4.3:29340623-29340642 MS.gene031619:CDS 50.0%
GAACGCACTGCAAAAAGCAG+TGG - chr4.3:29340672-29340691 MS.gene031619:CDS 50.0%
GATGCTGCAGAAGGACTTTG+TGG - chr4.3:29340913-29340932 MS.gene031619:CDS 50.0%
GGATTGGAGGAAGAAAGGAG+CGG - chr4.3:29340221-29340240 MS.gene031619:CDS 50.0%
GGGGTGAGGAAGGATACATT+AGG - chr4.3:29340875-29340894 MS.gene031619:CDS 50.0%
GGTGTAGACCAAGGTTGTGA+GGG - chr4.3:29340487-29340506 MS.gene031619:intron 50.0%
GTCATCTTGTAGAGTGCGAG+AGG + chr4.3:29339920-29339939 None:intergenic 50.0%
GTCGGATCCACTAGCATCAA+TGG + chr4.3:29340719-29340738 None:intergenic 50.0%
GTGCGTTCTCATTGTTGGCA+GGG + chr4.3:29340660-29340679 None:intergenic 50.0%
GTTCCGTCCACACCTTGATA+GGG + chr4.3:29340580-29340599 None:intergenic 50.0%
TAAGACCACCCTCACAACCT+TGG + chr4.3:29340498-29340517 None:intergenic 50.0%
TACCCAGTAATGGTAGCAGC+AGG + chr4.3:29340628-29340647 None:intergenic 50.0%
TACCCCTATCAAGGTGTGGA+CGG - chr4.3:29340574-29340593 MS.gene031619:CDS 50.0%
TCAGCTATTGCGGCAACTGA+AGG - chr4.3:29340394-29340413 MS.gene031619:CDS 50.0%
TGCGAGTGTTCCATCCACAA+TGG - chr4.3:29340200-29340219 MS.gene031619:CDS 50.0%
TGTTCCGTCCACACCTTGAT+AGG + chr4.3:29340581-29340600 None:intergenic 50.0%
TTCCGTCCACACCTTGATAG+GGG + chr4.3:29340579-29340598 None:intergenic 50.0%
! AGGTTGTGAGGGTGGTCTTA+TGG - chr4.3:29340498-29340517 MS.gene031619:intron 50.0%
!! GGATCATGGTGTTACTGCTG+TGG - chr4.3:29340789-29340808 MS.gene031619:CDS 50.0%
!! GGTGTCTTCACTGGTTCATG+TGG - chr4.3:29340757-29340776 MS.gene031619:CDS 50.0%
!! GGTGTTACTGCTGTGGGATA+TGG - chr4.3:29340796-29340815 MS.gene031619:CDS 50.0%
AACTCATGGGGAACCGACTG+GGG - chr4.3:29340856-29340875 MS.gene031619:CDS 55.0%
GAGCAATGGATGGCTCACCA+TGG - chr4.3:29339958-29339977 MS.gene031619:CDS 55.0%
GAGGCCGAATACCCCTATCA+AGG - chr4.3:29340565-29340584 MS.gene031619:CDS 55.0%
GCTCCTGCTGCTACCATTAC+TGG - chr4.3:29340622-29340641 MS.gene031619:CDS 55.0%
GTAGACCAAGGTTGTGAGGG+TGG - chr4.3:29340490-29340509 MS.gene031619:intron 55.0%
GTATCCTTCCTCACCCCAGT+CGG + chr4.3:29340872-29340891 None:intergenic 55.0%
! AGGGGTGTAGATGCTGCAGA+AGG - chr4.3:29340904-29340923 MS.gene031619:CDS 55.0%
ATGGGGAACCGACTGGGGTG+AGG - chr4.3:29340861-29340880 MS.gene031619:CDS 65.0%
GGAACCGACTGGGGTGAGGA+AGG - chr4.3:29340865-29340884 MS.gene031619:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 29339841 29340969 29339841 ID=MS.gene031619
chr4.3 mRNA 29339841 29340969 29339841 ID=MS.gene031619.t1;Parent=MS.gene031619
chr4.3 exon 29340537 29340969 29340537 ID=MS.gene031619.t1.exon1;Parent=MS.gene031619.t1
chr4.3 CDS 29340537 29340969 29340537 ID=cds.MS.gene031619.t1;Parent=MS.gene031619.t1
chr4.3 exon 29339841 29340433 29339841 ID=MS.gene031619.t1.exon2;Parent=MS.gene031619.t1
chr4.3 CDS 29339841 29340433 29339841 ID=cds.MS.gene031619.t1;Parent=MS.gene031619.t1
Gene Sequence

>MS.gene031619

ATGGCTGCCAATAATCAATTATATCATATTTCATTGGCATTGCTTTTATGCTTGGGATTGTGGGCAATTCAAGTCACCTCTCGCACTCTACAAGATGACCCTATGTATGAGAAACACGAGCAATGGATGGCTCACCATGGCAAAGTGTATAAGGATCTTCAAGAAAGAAAGAATCGTTTAAAGATATTCAAAGAAAATGTGAATTACATTGAAGCTTCCAACAATGCTAACAAGCCATACAAGCTTGGCATTAATCAATTTGCAGACCTCACCAATGAGGAGTTCATATCATCCAAAAATAGATTCAAGGGGCACATGTGCTCTTCAATCACCAGGACAACTACTTTTAAATATGAAAATGCGAGTGTTCCATCCACAATGGATTGGAGGAAGAAAGGAGCGGTGACACCCGTGAAAAATCAAGGTCAATGTGGTAAGTGTTTATCGAATAATTAATTCATTGAATTTCTTATAGATATATATGGTCAAGTTTGAATTGTCATCTGACTCCAATTATTTTAAATATGCCTTTATAGGATGTTGTTGGGCGTTTTCAGCTATTGCGGCAACTGAAGGGATTCATAAGTTAAGTACTGGAAAATTGGTGTCTTTATCCGAACAAGAACTTGTTGATTGTGACACAAAAGGTGTAGACCAAGGTTGTGAGGGTGGTCTTATGGATGATGCTTTCAAATTCATCAATCAAAATCATGGAATCAGTACTGAGGCCGAATACCCCTATCAAGGTGTGGACGGAACATGCAATGCTAATAAAGGTTCAGCTCCTGCTGCTACCATTACTGGGTATGAGGATGTCCCTGCCAACAATGAGAACGCACTGCAAAAAGCAGTGGCTAATCAACCAATTTCTGTAGCCATTGATGCTAGTGGATCCGACTTTCAGTTTTACAAAAGTGGTGTCTTCACTGGTTCATGTGGTACTGAGTTGGATCATGGTGTTACTGCTGTGGGATATGGCATTAACAATGATGGAACCAAATATTGGTTGGTTAAAAACTCATGGGGAACCGACTGGGGTGAGGAAGGATACATTAGGATGCAAAGGGGTGTAGATGCTGCAGAAGGACTTTGTGGCATTGCAATGCAGGCATCTTATCCTACGGCTTAA
Protein sequence

>MS.gene031619.t1

MAANNQLYHISLALLLCLGLWAIQVTSRTLQDDPMYEKHEQWMAHHGKVYKDLQERKNRLKIFKENVNYIEASNNANKPYKLGINQFADLTNEEFISSKNRFKGHMCSSITRTTTFKYENASVPSTMDWRKKGAVTPVKNQGQCGCCWAFSAIAATEGIHKLSTGKLVSLSEQELVDCDTKGVDQGCEGGLMDDAFKFINQNHGISTEAEYPYQGVDGTCNANKGSAPAATITGYEDVPANNENALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGINNDGTKYWLVKNSWGTDWGEEGYIRMQRGVDAAEGLCGIAMQASYPTA