AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031621

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031621.t1 MTR_4g079770 96.232 345 12 1 1 344 1 345 0.0 691
MS.gene031621.t1 MTR_4g079470 95.942 345 13 1 1 344 1 345 0.0 660
MS.gene031621.t1 MTR_5g022560 90.145 345 32 2 1 344 1 344 0.0 649
MS.gene031621.t1 MTR_4g107930 89.275 345 36 1 1 344 1 345 0.0 644
MS.gene031621.t1 MTR_4g079800 91.594 345 28 1 1 344 1 345 0.0 632
MS.gene031621.t1 MTR_4g047610 89.565 345 33 3 1 344 1 343 0.0 621
MS.gene031621.t1 MTR_2g075830 77.391 345 73 4 1 344 1 341 0.0 533
MS.gene031621.t1 MTR_4g080700 65.839 322 101 8 26 344 25 340 1.60e-150 427
MS.gene031621.t1 MTR_4g080730 65.217 322 103 7 26 344 25 340 1.32e-149 425
MS.gene031621.t1 MTR_4g080360 53.824 340 151 5 8 344 6 342 5.44e-131 377
MS.gene031621.t1 MTR_2g083930 52.722 349 151 5 1 342 1 342 1.02e-128 372
MS.gene031621.t1 MTR_4g104370 56.805 338 136 8 10 342 10 342 3.80e-128 371
MS.gene031621.t1 MTR_3g075390 53.372 341 144 8 13 342 7 343 1.64e-124 362
MS.gene031621.t1 MTR_4g079440 55.484 310 131 4 34 342 40 343 1.17e-122 357
MS.gene031621.t1 MTR_3g116080 52.035 344 151 7 7 343 11 347 1.34e-120 352
MS.gene031621.t1 MTR_7g078600 58.065 310 118 7 35 342 37 336 2.88e-120 350
MS.gene031621.t1 MTR_1g107550 56.013 316 129 7 33 342 43 354 7.19e-119 352
MS.gene031621.t1 MTR_7g079230 52.770 343 152 7 1 342 1 334 1.28e-118 346
MS.gene031621.t1 MTR_2g090890 50.146 343 158 5 7 343 1 336 6.69e-118 344
MS.gene031621.t1 MTR_4g094918 48.680 341 164 7 10 343 5 341 9.31e-115 337
MS.gene031621.t1 MTR_1g018840 48.098 368 152 9 4 342 3 360 1.81e-112 335
MS.gene031621.t1 MTR_1g018840 48.098 368 152 9 4 342 3 360 2.68e-112 335
MS.gene031621.t1 MTR_3g102220 51.682 327 139 8 28 342 41 360 1.30e-111 333
MS.gene031621.t1 MTR_3g112430 50.482 311 140 5 41 343 37 341 9.31e-103 309
MS.gene031621.t1 MTR_2g015850 55.172 290 122 6 56 342 78 362 7.81e-101 301
MS.gene031621.t1 MTR_2g016180 44.669 347 168 11 12 343 18 355 1.13e-88 275
MS.gene031621.t1 MTR_2g016270 44.606 343 159 11 12 343 18 340 3.38e-87 270
MS.gene031621.t1 MTR_2g067900 45.625 320 151 13 39 343 15 326 3.72e-77 240
MS.gene031621.t1 MTR_2g067560 45.171 321 144 13 39 338 15 324 8.99e-74 245
MS.gene031621.t1 MTR_4g125300 41.722 302 160 9 47 343 58 348 1.63e-70 224
MS.gene031621.t1 MTR_2g064475 38.710 372 162 17 17 343 1 351 7.60e-70 222
MS.gene031621.t1 MTR_2g015920 39.308 318 179 10 34 343 47 358 2.69e-68 219
MS.gene031621.t1 MTR_4g065077 38.438 320 181 8 33 343 44 356 6.58e-68 217
MS.gene031621.t1 MTR_7g078630 45.455 286 115 5 29 303 695 950 7.08e-67 226
MS.gene031621.t1 MTR_2g066980 35.775 355 166 13 11 343 4 318 2.26e-66 212
MS.gene031621.t1 MTR_4g071060 40.656 305 154 10 47 335 55 348 5.01e-65 210
MS.gene031621.t1 MTR_1g023210 38.348 339 167 12 22 335 31 352 1.05e-64 209
MS.gene031621.t1 MTR_8g086470 37.421 318 171 8 33 343 60 356 1.21e-62 204
MS.gene031621.t1 MTR_2g015930 39.623 318 171 12 38 343 50 358 7.06e-62 202
MS.gene031621.t1 MTR_1g023810 45.022 231 118 6 114 341 37 261 8.66e-62 198
MS.gene031621.t1 MTR_1g094135 36.202 337 177 12 24 335 35 358 1.82e-57 191
MS.gene031621.t1 MTR_4g072790 35.274 292 152 8 47 335 55 312 2.53e-45 157
MS.gene031621.t1 MTR_2g015840 50.633 158 73 4 7 161 1 156 2.48e-44 153
MS.gene031621.t1 MTR_2g015840 55.357 112 49 1 232 343 161 271 5.36e-36 132
MS.gene031621.t1 MTR_4g073660 34.797 296 152 10 47 335 54 315 1.75e-42 150
MS.gene031621.t1 MTR_4g072340 35.135 296 151 10 47 335 55 316 1.93e-42 150
MS.gene031621.t1 MTR_7g078580 64.286 98 34 1 245 342 4 100 1.52e-41 141
MS.gene031621.t1 MTR_7g079130 70.000 90 27 0 253 342 7 96 1.00e-40 138
MS.gene031621.t1 MTR_1g025450 38.503 187 90 7 114 296 43 208 1.19e-32 127
MS.gene031621.t1 MTR_2g016380 47.107 121 57 4 227 343 18 135 5.66e-27 107
MS.gene031621.t1 MTR_7g078310 42.958 142 71 3 81 219 113 247 5.97e-27 107
MS.gene031621.t1 MTR_7g111060 25.879 313 189 12 56 335 28 330 1.04e-22 97.8
MS.gene031621.t1 MTR_7g079260 61.111 72 12 1 269 340 7 62 1.23e-22 91.3
MS.gene031621.t1 MTR_7g111050 29.787 235 135 8 132 343 110 337 5.52e-22 95.5
MS.gene031621.t1 MTR_8g087510 41.985 131 69 5 167 295 23 148 8.01e-21 88.6
MS.gene031621.t1 MTR_3g029560 27.888 251 146 9 99 343 16 237 2.08e-20 89.0
MS.gene031621.t1 MTR_2g047960 48.235 85 43 1 135 219 7 90 8.51e-18 78.2
MS.gene031621.t1 MTR_3g082370 26.087 207 133 5 131 322 5 206 2.71e-17 80.1
MS.gene031621.t1 MTR_7g111050 27.692 195 117 6 132 305 105 296 1.33e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031621.t1 AT5G45890 55.652 345 143 5 7 343 3 345 3.45e-136 391
MS.gene031621.t1 AT5G50260 54.783 345 131 7 15 342 5 341 1.31e-129 375
MS.gene031621.t1 AT3G48340 54.335 346 135 8 13 342 4 342 6.54e-123 358
MS.gene031621.t1 AT2G34080 50.877 342 156 6 11 344 8 345 1.94e-121 353
MS.gene031621.t1 AT4G35350 51.163 344 151 8 10 343 16 352 1.56e-118 347
MS.gene031621.t1 AT5G43060 53.681 326 136 7 25 342 34 352 2.39e-116 345
MS.gene031621.t1 AT3G49340 50.146 343 151 7 17 344 4 341 1.73e-115 338
MS.gene031621.t1 AT2G27420 48.424 349 162 6 10 344 4 348 3.48e-115 338
MS.gene031621.t1 AT3G19390 49.854 343 161 6 9 343 6 345 4.57e-115 341
MS.gene031621.t1 AT3G48350 50.432 347 154 8 11 343 1 343 1.98e-114 336
MS.gene031621.t1 AT1G06260 51.594 345 147 7 8 342 7 341 2.02e-112 330
MS.gene031621.t1 AT1G20850 52.716 313 141 5 34 343 45 353 5.33e-112 330
MS.gene031621.t1 AT1G29090 46.459 353 173 7 4 344 7 355 1.52e-111 329
MS.gene031621.t1 AT4G36880 51.863 322 142 6 33 343 42 361 1.58e-111 329
MS.gene031621.t1 AT1G47128 51.780 309 136 7 40 342 50 351 1.89e-110 330
MS.gene031621.t1 AT3G19400 50.814 307 146 5 40 343 44 348 1.21e-109 324
MS.gene031621.t1 AT1G29080 46.132 349 162 9 13 344 7 346 3.64e-108 320
MS.gene031621.t1 AT1G09850 50.323 310 150 4 34 342 26 332 1.45e-103 311
MS.gene031621.t1 AT4G23520 49.359 312 137 9 41 342 48 348 1.88e-101 303
MS.gene031621.t1 AT4G11310 48.553 311 142 7 41 342 50 351 1.28e-99 298
MS.gene031621.t1 AT4G11320 46.984 315 141 7 41 342 57 358 2.26e-98 296
MS.gene031621.t1 AT4G11320 46.984 315 141 7 41 342 57 358 2.26e-98 296
MS.gene031621.t1 AT1G29110 41.880 351 166 9 9 343 5 333 3.04e-89 271
MS.gene031621.t1 AT3G43960 43.675 332 177 8 17 342 18 345 3.84e-86 265
MS.gene031621.t1 AT4G35350 51.406 249 109 6 34 277 45 286 3.97e-84 256
MS.gene031621.t1 AT5G60360 45.364 302 149 9 47 343 66 356 1.87e-78 244
MS.gene031621.t1 AT3G19400 48.963 241 119 4 40 277 44 283 7.49e-76 235
MS.gene031621.t1 AT5G60360 45.033 302 149 10 47 343 66 355 9.02e-76 237
MS.gene031621.t1 AT3G45310 43.894 303 154 9 47 344 66 357 3.58e-75 236
MS.gene031621.t1 AT3G48350 46.263 281 133 8 11 277 1 277 1.34e-74 232
MS.gene031621.t1 AT5G60360 44.710 293 146 9 47 334 66 347 4.98e-74 233
MS.gene031621.t1 AT3G45310 43.234 303 155 9 47 344 66 356 1.52e-72 229
MS.gene031621.t1 AT4G39090 40.260 308 152 11 47 335 58 352 6.68e-64 207
MS.gene031621.t1 AT2G21430 38.816 304 162 8 47 335 55 349 8.30e-64 207
MS.gene031621.t1 AT3G54940 39.241 316 154 10 43 335 54 354 7.53e-61 199
MS.gene031621.t1 AT4G16190 39.228 311 157 11 45 335 60 358 1.47e-60 199
MS.gene031621.t1 AT3G54940 39.738 229 120 5 43 259 54 276 1.66e-45 157
MS.gene031621.t1 AT1G02305 29.778 225 130 7 132 335 116 333 6.86e-24 101
MS.gene031621.t1 AT4G01610 27.148 291 177 11 76 339 52 334 1.77e-21 94.4
MS.gene031621.t1 AT4G01610 26.804 291 178 11 76 339 52 334 2.91e-20 90.9
MS.gene031621.t1 AT1G02300 29.384 211 123 6 145 335 146 350 7.99e-20 89.7
MS.gene031621.t1 AT2G22160 40.217 92 52 2 55 145 9 98 1.11e-14 69.7

Find 95 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATGATTTAATTGATTAT+TGG 0.217020 4.3:+29371739 None:intergenic
TACAAAAGTGGTGTCTTTAC+TGG 0.245613 4.3:-29370833 MS.gene031621:CDS
ACTTCCCAGTACTCAACTTA+TGG 0.259340 4.3:+29371161 None:intergenic
TTTCTCCAATCCACTGTTGA+TGG 0.267040 4.3:+29371368 None:intergenic
GCATCAATAGCTACGGAAAT+TGG 0.285597 4.3:+29370878 None:intergenic
CTTATTATTGCCAGCATTGT+TGG 0.324490 4.3:+29371526 None:intergenic
CTGCAGTGCCTGCTCATTGT+TGG 0.329361 4.3:+29370919 None:intergenic
AGTTCCGTCAACACCTTGAT+AGG 0.330791 4.3:+29371003 None:intergenic
TGGGAAGTTGGTCTCTTTAT+CGG 0.332823 4.3:-29371146 MS.gene031621:CDS
TTACTATGGCAAAGTCTATA+AGG 0.348730 4.3:-29371612 MS.gene031621:CDS
GAATTCATGGGGAACTGATT+GGG 0.351922 4.3:-29370726 MS.gene031621:CDS
ATCCATGCAGTTACCATTAC+CGG 0.356686 4.3:-29370959 MS.gene031621:CDS
AAATTCATCATTCAAAATCA+TGG 0.364764 4.3:-29371049 MS.gene031621:CDS
AGGCTGTGAGGGTGGCCTTA+TGG 0.366798 4.3:-29371083 MS.gene031621:CDS
ATTGGTTGGTTAAGAATTCA+TGG 0.370703 4.3:-29370739 MS.gene031621:CDS
ATTAAATCATAGCATTTCAT+TGG 0.375929 4.3:-29371729 MS.gene031621:CDS
CATTGACCTTGGTTCTTCAC+TGG 0.396716 4.3:+29371329 None:intergenic
AGAATTCATGGGGAACTGAT+TGG 0.403396 4.3:-29370727 MS.gene031621:CDS
GGAATCCATAAGTTGAGTAC+TGG 0.422547 4.3:-29371166 MS.gene031621:CDS
AGTACTTGTCTTTGTGATTG+AGG 0.434941 4.3:+29371415 None:intergenic
AGTGCCTGCTCATTGTTGGC+AGG 0.443934 4.3:+29370923 None:intergenic
ACTCAACTTATGGATTCCTT+CGG 0.446108 4.3:+29371171 None:intergenic
GGTGTCACTGCTGTAGGTTA+TGG 0.447580 4.3:-29370785 MS.gene031621:CDS
ATTCTTAACCAACCAATACT+TGG 0.453395 4.3:+29370745 None:intergenic
TCATAGCATTTCATTGGCAT+TGG 0.455936 4.3:-29371723 MS.gene031621:CDS
GACTTTCAGTTCTACAAAAG+TGG 0.468517 4.3:-29370845 MS.gene031621:CDS
GGAACTGATTGGGGTGAAGA+AGG 0.474210 4.3:-29370716 MS.gene031621:CDS
AAGTTTGGAACTTAAGCAGT+AGG 0.478514 4.3:+29370623 None:intergenic
GAGCAATGGATGAGTTACTA+TGG 0.479194 4.3:-29371626 MS.gene031621:CDS
TGCATCTAGAAACAAATTCA+AGG 0.484430 4.3:-29371450 MS.gene031621:CDS
AGATGCAATGAACTCCTCAT+TGG 0.488280 4.3:+29371466 None:intergenic
TTGCTCGTGTCTCTCGTACA+TGG 0.488893 4.3:+29371643 None:intergenic
TCTATAAGGATTCTCAAGAA+AGG 0.490614 4.3:-29371598 MS.gene031621:CDS
AAGAAAGGGAGAACCGTCTC+AGG 0.495449 4.3:-29371583 MS.gene031621:CDS
TACCCCTATCAAGGTGTTGA+CGG 0.498791 4.3:-29371007 MS.gene031621:CDS
GGGGTGAAGAAGGATACATT+AGG 0.500551 4.3:-29370706 MS.gene031621:CDS
GACATCTTCATACCCGGTAA+TGG 0.502057 4.3:+29370946 None:intergenic
AGGATACATTAGGATGCAAA+GGG 0.504604 4.3:-29370696 MS.gene031621:CDS
CTATAAGGATTCTCAAGAAA+GGG 0.505602 4.3:-29371597 MS.gene031621:CDS
ATTTGAAAGCATCATCCATA+AGG 0.506586 4.3:+29371068 None:intergenic
GATGCTGCTGAAGGACTTTG+TGG 0.515605 4.3:-29370668 MS.gene031621:CDS
GGTTATGGTGTCGGTAATGA+TGG 0.515702 4.3:-29370770 MS.gene031621:CDS
TCTCGTACATGGAGTCATCT+TGG 0.518580 4.3:+29371654 None:intergenic
TAAGTTGAGTACTGGGAAGT+TGG 0.520530 4.3:-29371158 MS.gene031621:CDS
AATAATAAGTCATACAAACT+TGG 0.521678 4.3:-29371512 MS.gene031621:CDS
TTGGTTGGTTAAGAATTCAT+GGG 0.526242 4.3:-29370738 MS.gene031621:CDS
GACACTTACCACATTGACCT+TGG 0.526963 4.3:+29371318 None:intergenic
ATTGAAGCTTCCAACAATGC+TGG 0.532605 4.3:-29371536 MS.gene031621:CDS
ACAGTGGATTGGAGAAAGAA+AGG 0.533216 4.3:-29371362 MS.gene031621:CDS
CTTGTTGATTGTGACACAAA+AGG 0.533495 4.3:-29371115 MS.gene031621:CDS
AAGGATACATTAGGATGCAA+AGG 0.533659 4.3:-29370697 MS.gene031621:CDS
TCTGCTGTTGCGGCAACCGA+AGG 0.538022 4.3:-29371187 MS.gene031621:CDS
TAAGGCCACCCTCACAGCCT+TGG 0.538822 4.3:+29371086 None:intergenic
GTTCCGTCAACACCTTGATA+GGG 0.541629 4.3:+29371004 None:intergenic
TAATTGATTATTGGCAGCCA+TGG 0.542700 4.3:+29371748 None:intergenic
GAATCCATAAGTTGAGTACT+GGG 0.546014 4.3:-29371165 MS.gene031621:CDS
GTGCCTGCTCATTGTTGGCA+GGG 0.546537 4.3:+29370924 None:intergenic
TCCGTAGCTATTGATGCTAG+TGG 0.550787 4.3:-29370872 MS.gene031621:CDS
GTGTTCCATCAACAGTGGAT+TGG 0.552593 4.3:-29371373 MS.gene031621:CDS
GAAGCTCAATACCCCTATCA+AGG 0.559311 4.3:-29371016 MS.gene031621:CDS
TGTCCCTGCCAACAATGAGC+AGG 0.564412 4.3:-29370927 MS.gene031621:CDS
CCGTAGCTATTGATGCTAGT+GGG 0.567886 4.3:-29370871 MS.gene031621:CDS
GTAATGATGGAACCAAGTAT+TGG 0.568388 4.3:-29370757 MS.gene031621:CDS
TGATGGAACCAAGTATTGGT+TGG 0.568906 4.3:-29370753 MS.gene031621:CDS
GGTGTAGACCAAGGCTGTGA+GGG 0.570213 4.3:-29371094 MS.gene031621:CDS
AGTTTGGAACTTAAGCAGTA+GGG 0.572870 4.3:+29370624 None:intergenic
AGGGGAGTCGATGCTGCTGA+AGG 0.575211 4.3:-29370677 MS.gene031621:CDS
AATTCATGGGGAACTGATTG+GGG 0.577168 4.3:-29370725 MS.gene031621:CDS
TTTCTTTGAATATCCTGAGA+CGG 0.585323 4.3:+29371570 None:intergenic
GTAGACCAAGGCTGTGAGGG+TGG 0.586935 4.3:-29371091 MS.gene031621:CDS
AGGACTTTGTGGCATTGCAA+TGG 0.587668 4.3:-29370657 MS.gene031621:CDS
CCCACTAGCATCAATAGCTA+CGG 0.596676 4.3:+29370871 None:intergenic
GGTAATGGTAACTGCATGGA+TGG 0.597553 4.3:+29370961 None:intergenic
ACCCGGTAATGGTAACTGCA+TGG 0.598149 4.3:+29370957 None:intergenic
TTCCGTCAACACCTTGATAG+GGG 0.605371 4.3:+29371005 None:intergenic
GGCAGGGACATCTTCATACC+CGG 0.606676 4.3:+29370940 None:intergenic
GCATCTAGAAACAAATTCAA+GGG 0.607899 4.3:-29371449 MS.gene031621:CDS
TGTACGAGAGACACGAGCAA+TGG 0.611237 4.3:-29371640 MS.gene031621:CDS
TGGTTCATGTGGAACTGAGT+TGG 0.612813 4.3:-29370813 MS.gene031621:CDS
GACCATGGTGTCACTGCTGT+AGG 0.612821 4.3:-29370791 MS.gene031621:CDS
TCCATGCAGTTACCATTACC+GGG 0.614442 4.3:-29370958 MS.gene031621:CDS
TGGTTGGTTAAGAATTCATG+GGG 0.622660 4.3:-29370737 MS.gene031621:CDS
TGCAAGTGTTCCATCAACAG+TGG 0.628871 4.3:-29371378 MS.gene031621:CDS
CAATGAACTCCTCATTGGTG+AGG 0.636198 4.3:+29371471 None:intergenic
GGTGTCTTTACTGGTTCATG+TGG 0.637602 4.3:-29370824 MS.gene031621:CDS
AACCTACAGCAGTGACACCA+TGG 0.649313 4.3:+29370789 None:intergenic
AGGTGTAGACCAAGGCTGTG+AGG 0.655227 4.3:-29371095 MS.gene031621:CDS
ATTTGCAGACCTCACCAATG+AGG 0.664553 4.3:-29371480 MS.gene031621:CDS
GTGAAGAACCAAGGTCAATG+TGG 0.671761 4.3:-29371326 MS.gene031621:intron
GCAGGCACTGCAGAAAGCTG+TGG 0.675386 4.3:-29370909 MS.gene031621:CDS
ACTGCTGTAGGTTATGGTGT+CGG 0.678447 4.3:-29370779 MS.gene031621:CDS
TGTGGAACTGAGTTGGACCA+TGG 0.679648 4.3:-29370806 MS.gene031621:CDS
GACACAAAAGGTGTAGACCA+AGG 0.702457 4.3:-29371103 MS.gene031621:CDS
GTAACACCAGTGAAGAACCA+AGG 0.718576 4.3:-29371335 MS.gene031621:CDS
GGATACATTAGGATGCAAAG+GGG 0.764149 4.3:-29370695 MS.gene031621:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTAATTTACCTTTTGT+AGG - chr4.3:29371154-29371173 MS.gene031621:CDS 15.0%
!! AAATTCATCATTCAAAATCA+TGG - chr4.3:29371331-29371350 MS.gene031621:CDS 20.0%
!! AATAATAAGTCATACAAACT+TGG - chr4.3:29370868-29370887 MS.gene031621:CDS 20.0%
!! ATTAAATCATAGCATTTCAT+TGG - chr4.3:29370651-29370670 MS.gene031621:CDS 20.0%
!! GCTATGATTTAATTGATTAT+TGG + chr4.3:29370644-29370663 None:intergenic 20.0%
ATTCTTAACCAACCAATACT+TGG + chr4.3:29371638-29371657 None:intergenic 30.0%
CTATAAGGATTCTCAAGAAA+GGG - chr4.3:29370783-29370802 MS.gene031621:CDS 30.0%
GCATCTAGAAACAAATTCAA+GGG - chr4.3:29370931-29370950 MS.gene031621:CDS 30.0%
TCTATAAGGATTCTCAAGAA+AGG - chr4.3:29370782-29370801 MS.gene031621:CDS 30.0%
TGCATCTAGAAACAAATTCA+AGG - chr4.3:29370930-29370949 MS.gene031621:CDS 30.0%
TTACTATGGCAAAGTCTATA+AGG - chr4.3:29370768-29370787 MS.gene031621:CDS 30.0%
TTTCTTTGAATATCCTGAGA+CGG + chr4.3:29370813-29370832 None:intergenic 30.0%
! ATTGGTTGGTTAAGAATTCA+TGG - chr4.3:29371641-29371660 MS.gene031621:CDS 30.0%
! TTGGTTGGTTAAGAATTCAT+GGG - chr4.3:29371642-29371661 MS.gene031621:CDS 30.0%
!! ATTTGAAAGCATCATCCATA+AGG + chr4.3:29371315-29371334 None:intergenic 30.0%
AAGGATACATTAGGATGCAA+AGG - chr4.3:29371683-29371702 MS.gene031621:CDS 35.0%
ACTCAACTTATGGATTCCTT+CGG + chr4.3:29371212-29371231 None:intergenic 35.0%
AGGATACATTAGGATGCAAA+GGG - chr4.3:29371684-29371703 MS.gene031621:CDS 35.0%
AGTACTTGTCTTTGTGATTG+AGG + chr4.3:29370968-29370987 None:intergenic 35.0%
CTTATTATTGCCAGCATTGT+TGG + chr4.3:29370857-29370876 None:intergenic 35.0%
GACTTTCAGTTCTACAAAAG+TGG - chr4.3:29371535-29371554 MS.gene031621:CDS 35.0%
TACAAAAGTGGTGTCTTTAC+TGG - chr4.3:29371547-29371566 MS.gene031621:CDS 35.0%
TCATAGCATTTCATTGGCAT+TGG - chr4.3:29370657-29370676 MS.gene031621:CDS 35.0%
! ACCTTTTGTAGGATGTTGTT+GGG - chr4.3:29371165-29371184 MS.gene031621:CDS 35.0%
! CTTGTTGATTGTGACACAAA+AGG - chr4.3:29371265-29371284 MS.gene031621:intron 35.0%
! GAATCCATAAGTTGAGTACT+GGG - chr4.3:29371215-29371234 MS.gene031621:intron 35.0%
! GTAATGATGGAACCAAGTAT+TGG - chr4.3:29371623-29371642 MS.gene031621:CDS 35.0%
! TACCTTTTGTAGGATGTTGT+TGG - chr4.3:29371164-29371183 MS.gene031621:CDS 35.0%
! TGGTTGGTTAAGAATTCATG+GGG - chr4.3:29371643-29371662 MS.gene031621:CDS 35.0%
!!! ATTGGCATTGGTTTTATGCT+TGG - chr4.3:29370669-29370688 MS.gene031621:CDS 35.0%
!!! TTGGCATTGGTTTTATGCTT+GGG - chr4.3:29370670-29370689 MS.gene031621:CDS 35.0%
AATTCATGGGGAACTGATTG+GGG - chr4.3:29371655-29371674 MS.gene031621:CDS 40.0%
ACTTCCCAGTACTCAACTTA+TGG + chr4.3:29371222-29371241 None:intergenic 40.0%
AGAATTCATGGGGAACTGAT+TGG - chr4.3:29371653-29371672 MS.gene031621:CDS 40.0%
AGATGCAATGAACTCCTCAT+TGG + chr4.3:29370917-29370936 None:intergenic 40.0%
ATCCATGCAGTTACCATTAC+CGG - chr4.3:29371421-29371440 MS.gene031621:CDS 40.0%
ATTGAAGCTTCCAACAATGC+TGG - chr4.3:29370844-29370863 MS.gene031621:CDS 40.0%
GAATTCATGGGGAACTGATT+GGG - chr4.3:29371654-29371673 MS.gene031621:CDS 40.0%
GAGCAATGGATGAGTTACTA+TGG - chr4.3:29370754-29370773 MS.gene031621:CDS 40.0%
GCATCAATAGCTACGGAAAT+TGG + chr4.3:29371505-29371524 None:intergenic 40.0%
GGATACATTAGGATGCAAAG+GGG - chr4.3:29371685-29371704 MS.gene031621:CDS 40.0%
TTTCTCCAATCCACTGTTGA+TGG + chr4.3:29371015-29371034 None:intergenic 40.0%
! GGAATCCATAAGTTGAGTAC+TGG - chr4.3:29371214-29371233 MS.gene031621:intron 40.0%
! TGGGAAGTTGGTCTCTTTAT+CGG - chr4.3:29371234-29371253 MS.gene031621:intron 40.0%
!! ACAGTGGATTGGAGAAAGAA+AGG - chr4.3:29371018-29371037 MS.gene031621:CDS 40.0%
!! GGTTTTATGCTTGGGATTGT+TGG - chr4.3:29370678-29370697 MS.gene031621:CDS 40.0%
!! TAAGTTGAGTACTGGGAAGT+TGG - chr4.3:29371222-29371241 MS.gene031621:intron 40.0%
!! TGATGGAACCAAGTATTGGT+TGG - chr4.3:29371627-29371646 MS.gene031621:CDS 40.0%
ACTGCTGTAGGTTATGGTGT+CGG - chr4.3:29371601-29371620 MS.gene031621:CDS 45.0%
AGGACTTTGTGGCATTGCAA+TGG - chr4.3:29371723-29371742 MS.gene031621:CDS 45.0%
AGTTCCGTCAACACCTTGAT+AGG + chr4.3:29371380-29371399 None:intergenic 45.0%
ATTTGCAGACCTCACCAATG+AGG - chr4.3:29370900-29370919 MS.gene031621:CDS 45.0%
CAATGAACTCCTCATTGGTG+AGG + chr4.3:29370912-29370931 None:intergenic 45.0%
CCCACTAGCATCAATAGCTA+CGG + chr4.3:29371512-29371531 None:intergenic 45.0%
CCGTAGCTATTGATGCTAGT+GGG - chr4.3:29371509-29371528 MS.gene031621:CDS 45.0%
GAAGCTCAATACCCCTATCA+AGG - chr4.3:29371364-29371383 MS.gene031621:CDS 45.0%
GACACTTACCACATTGACCT+TGG + chr4.3:29371065-29371084 None:intergenic 45.0%
GACATCTTCATACCCGGTAA+TGG + chr4.3:29371437-29371456 None:intergenic 45.0%
GCCCAACAACATCCTACAAA+AGG + chr4.3:29371169-29371188 None:intergenic 45.0%
GGGGTGAAGAAGGATACATT+AGG - chr4.3:29371674-29371693 MS.gene031621:CDS 45.0%
GTAACACCAGTGAAGAACCA+AGG - chr4.3:29371045-29371064 MS.gene031621:CDS 45.0%
GTGAAGAACCAAGGTCAATG+TGG - chr4.3:29371054-29371073 MS.gene031621:CDS 45.0%
GTGTTCCATCAACAGTGGAT+TGG - chr4.3:29371007-29371026 MS.gene031621:CDS 45.0%
GTTCCGTCAACACCTTGATA+GGG + chr4.3:29371379-29371398 None:intergenic 45.0%
TCCATGCAGTTACCATTACC+GGG - chr4.3:29371422-29371441 MS.gene031621:CDS 45.0%
TCCGTAGCTATTGATGCTAG+TGG - chr4.3:29371508-29371527 MS.gene031621:CDS 45.0%
TCTCGTACATGGAGTCATCT+TGG + chr4.3:29370729-29370748 None:intergenic 45.0%
TGCAAGTGTTCCATCAACAG+TGG - chr4.3:29371002-29371021 MS.gene031621:CDS 45.0%
TGGTTCATGTGGAACTGAGT+TGG - chr4.3:29371567-29371586 MS.gene031621:CDS 45.0%
TTCCGTCAACACCTTGATAG+GGG + chr4.3:29371378-29371397 None:intergenic 45.0%
! CATTGACCTTGGTTCTTCAC+TGG + chr4.3:29371054-29371073 None:intergenic 45.0%
! GACACAAAAGGTGTAGACCA+AGG - chr4.3:29371277-29371296 MS.gene031621:intron 45.0%
! GGTAATGGTAACTGCATGGA+TGG + chr4.3:29371422-29371441 None:intergenic 45.0%
! TTGGGCATTTTCTGCTGTTG+CGG - chr4.3:29371183-29371202 MS.gene031621:CDS 45.0%
!! GGTGTCTTTACTGGTTCATG+TGG - chr4.3:29371556-29371575 MS.gene031621:CDS 45.0%
!! GGTTATGGTGTCGGTAATGA+TGG - chr4.3:29371610-29371629 MS.gene031621:CDS 45.0%
!! TACCCCTATCAAGGTGTTGA+CGG - chr4.3:29371373-29371392 MS.gene031621:CDS 45.0%
AACCTACAGCAGTGACACCA+TGG + chr4.3:29371594-29371613 None:intergenic 50.0%
AAGAAAGGGAGAACCGTCTC+AGG - chr4.3:29370797-29370816 MS.gene031621:CDS 50.0%
GATGCTGCTGAAGGACTTTG+TGG - chr4.3:29371712-29371731 MS.gene031621:CDS 50.0%
GGAACTGATTGGGGTGAAGA+AGG - chr4.3:29371664-29371683 MS.gene031621:CDS 50.0%
TGTACGAGAGACACGAGCAA+TGG - chr4.3:29370740-29370759 MS.gene031621:CDS 50.0%
TTGCTCGTGTCTCTCGTACA+TGG + chr4.3:29370740-29370759 None:intergenic 50.0%
! ACCCGGTAATGGTAACTGCA+TGG + chr4.3:29371426-29371445 None:intergenic 50.0%
! TGTGGAACTGAGTTGGACCA+TGG - chr4.3:29371574-29371593 MS.gene031621:CDS 50.0%
!! GGTGTCACTGCTGTAGGTTA+TGG - chr4.3:29371595-29371614 MS.gene031621:CDS 50.0%
AGGTGTAGACCAAGGCTGTG+AGG - chr4.3:29371285-29371304 MS.gene031621:intron 55.0%
AGTGCCTGCTCATTGTTGGC+AGG + chr4.3:29371460-29371479 None:intergenic 55.0%
CTGCAGTGCCTGCTCATTGT+TGG + chr4.3:29371464-29371483 None:intergenic 55.0%
GGCAGGGACATCTTCATACC+CGG + chr4.3:29371443-29371462 None:intergenic 55.0%
GGTGTAGACCAAGGCTGTGA+GGG - chr4.3:29371286-29371305 MS.gene031621:intron 55.0%
GTGCCTGCTCATTGTTGGCA+GGG + chr4.3:29371459-29371478 None:intergenic 55.0%
TGTCCCTGCCAACAATGAGC+AGG - chr4.3:29371453-29371472 MS.gene031621:CDS 55.0%
!! GACCATGGTGTCACTGCTGT+AGG - chr4.3:29371589-29371608 MS.gene031621:CDS 55.0%
AGGGGAGTCGATGCTGCTGA+AGG - chr4.3:29371703-29371722 MS.gene031621:CDS 60.0%
GTAGACCAAGGCTGTGAGGG+TGG - chr4.3:29371289-29371308 MS.gene031621:intron 60.0%
TAAGGCCACCCTCACAGCCT+TGG + chr4.3:29371297-29371316 None:intergenic 60.0%
TCTGCTGTTGCGGCAACCGA+AGG - chr4.3:29371193-29371212 MS.gene031621:CDS 60.0%
! AGGCTGTGAGGGTGGCCTTA+TGG - chr4.3:29371297-29371316 MS.gene031621:intron 60.0%
! GCAGGCACTGCAGAAAGCTG+TGG - chr4.3:29371471-29371490 MS.gene031621:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 29370634 29371768 29370634 ID=MS.gene031621
chr4.3 mRNA 29370634 29371768 29370634 ID=MS.gene031621.t1;Parent=MS.gene031621
chr4.3 exon 29371327 29371768 29371327 ID=MS.gene031621.t1.exon1;Parent=MS.gene031621.t1
chr4.3 CDS 29371327 29371768 29371327 ID=cds.MS.gene031621.t1;Parent=MS.gene031621.t1
chr4.3 exon 29370634 29371226 29370634 ID=MS.gene031621.t1.exon2;Parent=MS.gene031621.t1
chr4.3 CDS 29370634 29371226 29370634 ID=cds.MS.gene031621.t1;Parent=MS.gene031621.t1
Gene Sequence

>MS.gene031621

ATGGCTGCCAATAATCAATTAAATCATAGCATTTCATTGGCATTGGTTTTATGCTTGGGATTGTTGGCTATTCAAGTCACTTCTCGTACTCTCCAAGATGACTCCATGTACGAGAGACACGAGCAATGGATGAGTTACTATGGCAAAGTCTATAAGGATTCTCAAGAAAGGGAGAACCGTCTCAGGATATTCAAAGAAAATGTGAATTACATTGAAGCTTCCAACAATGCTGGCAATAATAAGTCATACAAACTTGGCATTAATCAATTTGCAGACCTCACCAATGAGGAGTTCATTGCATCTAGAAACAAATTCAAGGGTCATATGTGCTCCTCAATCACAAAGACAAGTACTTTTAAGTATGAAAATGCAAGTGTTCCATCAACAGTGGATTGGAGAAAGAAAGGAGCAGTAACACCAGTGAAGAACCAAGGTCAATGTGGTAAGTGTCTATCTAATATGTAATGCATTCAATTACATATTATGTGATAAAAATTTGAAGTGTCATTAAACTCTAGTTAATTTTAATTTACCTTTTGTAGGATGTTGTTGGGCATTTTCTGCTGTTGCGGCAACCGAAGGAATCCATAAGTTGAGTACTGGGAAGTTGGTCTCTTTATCGGAACAAGAACTTGTTGATTGTGACACAAAAGGTGTAGACCAAGGCTGTGAGGGTGGCCTTATGGATGATGCTTTCAAATTCATCATTCAAAATCATGGACTCAACACTGAAGCTCAATACCCCTATCAAGGTGTTGACGGAACTTGCAGTGCAAACAAAGCATCCATCCATGCAGTTACCATTACCGGGTATGAAGATGTCCCTGCCAACAATGAGCAGGCACTGCAGAAAGCTGTGGCAAATCAACCAATTTCCGTAGCTATTGATGCTAGTGGGTCTGACTTTCAGTTCTACAAAAGTGGTGTCTTTACTGGTTCATGTGGAACTGAGTTGGACCATGGTGTCACTGCTGTAGGTTATGGTGTCGGTAATGATGGAACCAAGTATTGGTTGGTTAAGAATTCATGGGGAACTGATTGGGGTGAAGAAGGATACATTAGGATGCAAAGGGGAGTCGATGCTGCTGAAGGACTTTGTGGCATTGCAATGGAAGCATCTTACCCTACTGCTTAA
Protein sequence

>MS.gene031621.t1

MAANNQLNHSISLALVLCLGLLAIQVTSRTLQDDSMYERHEQWMSYYGKVYKDSQERENRLRIFKENVNYIEASNNAGNNKSYKLGINQFADLTNEEFIASRNKFKGHMCSSITKTSTFKYENASVPSTVDWRKKGAVTPVKNQGQCGCCWAFSAVAATEGIHKLSTGKLVSLSEQELVDCDTKGVDQGCEGGLMDDAFKFIIQNHGLNTEAQYPYQGVDGTCSANKASIHAVTITGYEDVPANNEQALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGVGNDGTKYWLVKNSWGTDWGEEGYIRMQRGVDAAEGLCGIAMEASYPTA