AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045503

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045503.t1 MTR_4g079770 94.203 345 19 1 1 344 1 345 0.0 677
MS.gene045503.t1 MTR_4g079470 93.913 345 20 1 1 344 1 345 0.0 646
MS.gene045503.t1 MTR_5g022560 89.855 345 33 2 1 344 1 344 0.0 645
MS.gene045503.t1 MTR_4g107930 88.696 345 38 1 1 344 1 345 0.0 639
MS.gene045503.t1 MTR_4g079800 91.304 345 29 1 1 344 1 345 0.0 632
MS.gene045503.t1 MTR_4g047610 88.986 345 35 3 1 344 1 343 0.0 617
MS.gene045503.t1 MTR_2g075830 78.261 345 70 4 1 344 1 341 0.0 538
MS.gene045503.t1 MTR_4g080700 66.044 321 102 7 26 344 25 340 1.25e-153 435
MS.gene045503.t1 MTR_4g080730 66.044 321 102 6 26 344 25 340 1.38e-153 435
MS.gene045503.t1 MTR_4g104370 57.692 338 133 8 10 342 10 342 6.86e-132 380
MS.gene045503.t1 MTR_4g080360 54.118 340 150 5 8 344 6 342 1.47e-130 377
MS.gene045503.t1 MTR_2g083930 53.009 349 150 5 1 342 1 342 1.74e-129 374
MS.gene045503.t1 MTR_3g075390 53.666 341 143 7 13 342 7 343 1.03e-126 367
MS.gene045503.t1 MTR_4g079440 55.806 310 130 4 34 342 40 343 2.38e-122 356
MS.gene045503.t1 MTR_3g116080 51.744 344 152 7 7 343 11 347 1.56e-120 351
MS.gene045503.t1 MTR_1g107550 55.238 315 133 5 33 342 43 354 4.56e-119 352
MS.gene045503.t1 MTR_7g079230 52.187 343 154 7 1 342 1 334 3.37e-118 345
MS.gene045503.t1 MTR_2g090890 49.854 343 159 5 7 343 1 336 6.99e-118 344
MS.gene045503.t1 MTR_7g078600 57.097 310 121 7 35 342 37 336 4.88e-117 342
MS.gene045503.t1 MTR_4g094918 49.123 342 161 7 10 343 5 341 2.96e-115 338
MS.gene045503.t1 MTR_1g018840 47.671 365 158 8 4 342 3 360 2.73e-112 334
MS.gene045503.t1 MTR_1g018840 47.671 365 158 8 4 342 3 360 3.15e-112 335
MS.gene045503.t1 MTR_3g102220 51.682 327 139 8 28 342 41 360 4.03e-111 332
MS.gene045503.t1 MTR_3g112430 50.161 311 141 5 41 343 37 341 5.87e-102 307
MS.gene045503.t1 MTR_2g015850 55.517 290 121 6 56 342 78 362 1.52e-100 301
MS.gene045503.t1 MTR_2g016180 44.092 347 170 10 12 343 18 355 5.78e-89 275
MS.gene045503.t1 MTR_2g016270 44.606 343 159 11 12 343 18 340 2.15e-87 271
MS.gene045503.t1 MTR_2g067900 45.000 320 153 12 39 343 15 326 4.82e-78 242
MS.gene045503.t1 MTR_2g067560 45.171 321 144 13 39 338 15 324 2.35e-73 244
MS.gene045503.t1 MTR_2g064475 38.978 372 161 17 17 343 1 351 4.99e-71 225
MS.gene045503.t1 MTR_4g125300 40.717 307 166 9 42 343 53 348 1.29e-68 219
MS.gene045503.t1 MTR_2g015920 38.994 318 180 10 34 343 47 358 4.81e-68 218
MS.gene045503.t1 MTR_4g065077 39.009 323 175 10 33 343 44 356 3.67e-67 215
MS.gene045503.t1 MTR_2g066980 34.930 355 169 12 11 343 4 318 5.90e-66 211
MS.gene045503.t1 MTR_4g071060 40.127 314 162 10 38 335 45 348 1.20e-65 211
MS.gene045503.t1 MTR_7g078630 44.406 286 118 5 29 303 695 950 1.23e-64 220
MS.gene045503.t1 MTR_1g023210 38.643 339 166 12 22 335 31 352 2.02e-64 208
MS.gene045503.t1 MTR_1g023810 45.455 231 117 6 114 341 37 261 8.35e-63 201
MS.gene045503.t1 MTR_2g015930 39.564 321 167 12 38 343 50 358 3.75e-62 202
MS.gene045503.t1 MTR_8g086470 38.006 321 165 10 33 343 60 356 8.79e-62 201
MS.gene045503.t1 MTR_1g094135 35.608 337 179 12 24 335 35 358 1.12e-55 186
MS.gene045503.t1 MTR_4g072790 34.343 297 158 8 42 335 50 312 2.70e-45 157
MS.gene045503.t1 MTR_2g015840 50.633 158 73 4 7 161 1 156 2.07e-44 154
MS.gene045503.t1 MTR_2g015840 55.000 120 52 2 225 343 153 271 4.90e-37 134
MS.gene045503.t1 MTR_4g073660 33.887 301 158 9 42 335 49 315 2.47e-43 152
MS.gene045503.t1 MTR_4g072340 33.887 301 158 9 42 335 50 316 3.02e-43 152
MS.gene045503.t1 MTR_7g078580 64.286 98 34 1 245 342 4 100 1.51e-41 141
MS.gene045503.t1 MTR_7g079130 70.000 90 27 0 253 342 7 96 1.09e-40 138
MS.gene045503.t1 MTR_1g025450 38.503 187 90 7 114 296 43 208 1.37e-32 127
MS.gene045503.t1 MTR_7g078310 44.366 142 69 3 81 219 113 247 1.60e-27 108
MS.gene045503.t1 MTR_2g016380 46.281 121 58 4 227 343 18 135 2.41e-26 105
MS.gene045503.t1 MTR_7g111060 26.032 315 186 12 56 335 28 330 1.95e-23 99.8
MS.gene045503.t1 MTR_7g079260 61.111 72 12 1 269 340 7 62 1.43e-22 91.3
MS.gene045503.t1 MTR_7g111050 29.362 235 136 8 132 343 110 337 1.01e-21 94.7
MS.gene045503.t1 MTR_3g029560 28.968 252 142 10 99 343 16 237 9.80e-21 90.1
MS.gene045503.t1 MTR_8g087510 41.985 131 69 5 167 295 23 148 1.72e-20 87.8
MS.gene045503.t1 MTR_2g047960 48.235 85 43 1 135 219 7 90 8.01e-18 78.2
MS.gene045503.t1 MTR_7g111050 27.179 195 118 6 132 305 105 296 1.81e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045503.t1 AT5G45890 55.652 345 143 5 7 343 3 345 2.59e-136 391
MS.gene045503.t1 AT5G50260 55.652 345 128 7 15 342 5 341 2.61e-133 384
MS.gene045503.t1 AT3G48340 54.070 344 139 7 13 342 4 342 9.61e-125 362
MS.gene045503.t1 AT2G34080 51.462 342 154 6 11 344 8 345 3.35e-124 360
MS.gene045503.t1 AT5G43060 53.988 326 135 7 25 342 34 352 1.21e-116 345
MS.gene045503.t1 AT4G35350 53.651 315 134 6 34 343 45 352 1.40e-116 342
MS.gene045503.t1 AT3G19390 50.146 343 160 6 9 343 6 345 1.70e-116 345
MS.gene045503.t1 AT3G49340 50.437 343 150 7 17 344 4 341 8.23e-116 339
MS.gene045503.t1 AT3G48350 50.720 347 153 8 11 343 1 343 9.49e-116 340
MS.gene045503.t1 AT2G27420 48.711 349 161 6 10 344 4 348 9.60e-115 337
MS.gene045503.t1 AT1G29090 46.742 353 172 7 4 344 7 355 4.65e-113 333
MS.gene045503.t1 AT1G06260 51.594 345 147 7 8 342 7 341 6.49e-113 332
MS.gene045503.t1 AT1G20850 52.077 313 143 5 34 343 45 353 7.89e-112 330
MS.gene045503.t1 AT4G36880 50.311 322 147 6 33 343 42 361 3.99e-111 328
MS.gene045503.t1 AT1G47128 52.104 309 135 7 40 342 50 351 8.11e-111 330
MS.gene045503.t1 AT3G19400 50.814 307 146 5 40 343 44 348 9.35e-111 327
MS.gene045503.t1 AT1G29080 45.559 349 164 8 13 344 7 346 3.56e-108 320
MS.gene045503.t1 AT1G09850 49.051 316 145 5 34 342 26 332 2.05e-103 311
MS.gene045503.t1 AT4G23520 48.867 309 143 8 41 342 48 348 4.99e-101 302
MS.gene045503.t1 AT4G11310 47.910 311 144 7 41 342 50 351 4.09e-98 295
MS.gene045503.t1 AT4G11320 46.349 315 143 7 41 342 57 358 9.54e-97 291
MS.gene045503.t1 AT4G11320 46.349 315 143 7 41 342 57 358 9.54e-97 291
MS.gene045503.t1 AT1G29110 42.165 351 165 9 9 343 5 333 5.98e-91 275
MS.gene045503.t1 AT3G43960 43.505 331 179 7 17 342 18 345 7.29e-85 261
MS.gene045503.t1 AT4G35350 51.004 249 110 6 34 277 45 286 2.56e-82 252
MS.gene045503.t1 AT3G19400 48.963 241 119 4 40 277 44 283 5.05e-77 238
MS.gene045503.t1 AT3G48350 46.619 281 132 8 11 277 1 277 5.93e-76 236
MS.gene045503.t1 AT5G60360 43.506 308 158 9 42 344 61 357 2.04e-75 236
MS.gene045503.t1 AT5G60360 43.182 308 158 10 42 344 61 356 6.78e-73 230
MS.gene045503.t1 AT3G45310 41.883 308 163 9 42 344 61 357 8.05e-72 227
MS.gene045503.t1 AT5G60360 42.761 297 158 8 42 333 61 350 3.12e-71 226
MS.gene045503.t1 AT3G45310 41.558 308 163 10 42 344 61 356 2.70e-69 221
MS.gene045503.t1 AT2G21430 38.816 304 162 8 47 335 55 349 5.23e-64 207
MS.gene045503.t1 AT4G39090 40.260 308 152 11 47 335 58 352 3.75e-63 205
MS.gene045503.t1 AT4G16190 39.032 310 159 9 45 335 60 358 2.12e-60 198
MS.gene045503.t1 AT3G54940 38.438 320 159 10 39 335 50 354 6.06e-60 197
MS.gene045503.t1 AT3G54940 39.301 229 121 5 43 259 54 276 1.31e-44 154
MS.gene045503.t1 AT1G02305 26.703 367 200 13 3 335 2 333 3.77e-25 104
MS.gene045503.t1 AT4G01610 27.119 295 172 11 76 339 52 334 5.98e-22 95.5
MS.gene045503.t1 AT4G01610 26.780 295 173 11 76 339 52 334 9.60e-21 92.4
MS.gene045503.t1 AT1G02300 29.108 213 121 6 145 335 146 350 4.19e-20 90.5
MS.gene045503.t1 AT2G22160 40.217 92 52 2 55 145 9 98 4.26e-15 70.9

Find 99 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATGATTTAATTGATTAT+TGG 0.217020 4.2:+30423158 None:intergenic
GCCCCTGCTGCTACCATTAC+TGG 0.247727 4.2:-30422378 MS.gene045503:CDS
TACAAAAGTGGTGTCTTTAC+TGG 0.247785 4.2:-30422252 MS.gene045503:CDS
GCATCAATGGCTACGGAAAT+TGG 0.280161 4.2:+30422297 None:intergenic
CTTATTATTGCCAGCATTGT+TGG 0.324490 4.2:+30422945 None:intergenic
TGGGAAGTTGGTCTCTTTAT+CGG 0.332823 4.2:-30422565 MS.gene045503:CDS
TGTTCCGTCCACACCTTGAT+AGG 0.347233 4.2:+30422422 None:intergenic
GAATTCATGGGGAACTGATT+GGG 0.351922 4.2:-30422145 MS.gene045503:CDS
AAGAAAGGGAGAACCGTCTT+AGG 0.352861 4.2:-30423002 MS.gene045503:CDS
TCACTATGGCAAAGTCTATA+AGG 0.352872 4.2:-30423031 MS.gene045503:CDS
AAATTCATCATTCAAAATCA+TGG 0.364764 4.2:-30422468 MS.gene045503:CDS
ATTGGTTGGTTAAGAATTCA+TGG 0.370703 4.2:-30422158 MS.gene045503:CDS
ATTAAATCATAGCATTTCAT+TGG 0.375929 4.2:-30423148 MS.gene045503:CDS
ACCTATTGTAGGATGTTGTT+GGG 0.383083 4.2:-30422634 MS.gene045503:intron
AGGTTGTGAGGGTGGCCTTA+TGG 0.383116 4.2:-30422502 MS.gene045503:CDS
TTGCAATGCCTGCTCATTGT+TGG 0.384601 4.2:+30422338 None:intergenic
CATTGACCTTGGTTCTTCAC+TGG 0.396716 4.2:+30422748 None:intergenic
AGAATTCATGGGGAACTGAT+TGG 0.403396 4.2:-30422146 MS.gene045503:CDS
GGAATTCATAAGTTGAGTAC+TGG 0.406869 4.2:-30422585 MS.gene045503:CDS
TACCTATTGTAGGATGTTGT+TGG 0.413643 4.2:-30422635 MS.gene045503:intron
GGAACATGCAATGCTAATAA+AGG 0.418975 4.2:-30422405 MS.gene045503:CDS
GGTGTCACTGCCGTTGGTTA+TGG 0.425588 4.2:-30422204 MS.gene045503:CDS
AGTAGTTGTCTTTGTGATTG+AGG 0.437424 4.2:+30422834 None:intergenic
ACTCAACTTATGAATTCCTT+CGG 0.443283 4.2:+30422590 None:intergenic
GGGGTGAGGAAGGATACATT+AGG 0.445488 4.2:-30422125 MS.gene045503:CDS
TTTCTCCAATCCACTGCTGA+TGG 0.445961 4.2:+30422787 None:intergenic
AATGCCTGCTCATTGTTGGC+AGG 0.451756 4.2:+30422342 None:intergenic
ATTCTTAACCAACCAATACT+TGG 0.453395 4.2:+30422164 None:intergenic
TCATAGCATTTCATTGGCAT+TGG 0.455936 4.2:-30423142 MS.gene045503:CDS
GTCAGACCCACTAGCATCAA+TGG 0.473799 4.2:+30422284 None:intergenic
GGTTATGGTGTTGGTAATGA+TGG 0.479044 4.2:-30422189 MS.gene045503:CDS
GGAACTGATTGGGGTGAGGA+AGG 0.483892 4.2:-30422135 MS.gene045503:CDS
CTTGTTGATTGCGACACAAA+AGG 0.484366 4.2:-30422534 MS.gene045503:CDS
AGCATCTAGAAACAAATTCA+AGG 0.484430 4.2:-30422869 MS.gene045503:CDS
AGATGCTATGAACTCCTCAT+TGG 0.488280 4.2:+30422885 None:intergenic
TTGCTCGTGTCTCTCGTACA+TGG 0.488893 4.2:+30423062 None:intergenic
TAAGTTGAGTACTGGGAAGT+TGG 0.489338 4.2:-30422577 MS.gene045503:CDS
TCTATAAGGATTCTCAAGAA+AGG 0.490614 4.2:-30423017 MS.gene045503:CDS
TGCTACCATTACTGGGTATG+AGG 0.491803 4.2:-30422370 MS.gene045503:CDS
AAGTTTGACACTTAAGCAGT+AGG 0.491983 4.2:+30422042 None:intergenic
TACCCAGTAATGGTAGCAGC+AGG 0.503313 4.2:+30422375 None:intergenic
CTATAAGGATTCTCAAGAAA+GGG 0.505602 4.2:-30423016 MS.gene045503:CDS
ATTTGAAAGCATCATCCATA+AGG 0.506586 4.2:+30422487 None:intergenic
GCAGTGGATTGGAGAAAGAA+AGG 0.507676 4.2:-30422781 MS.gene045503:CDS
GATGCTGCTGAAGGACTTTG+TGG 0.515605 4.2:-30422087 MS.gene045503:CDS
GTTCCGTCCACACCTTGATA+GGG 0.517808 4.2:+30422423 None:intergenic
CCCCTGCTGCTACCATTACT+GGG 0.517972 4.2:-30422377 MS.gene045503:CDS
TCTCGTACATGGAGTCATCT+TGG 0.518580 4.2:+30423073 None:intergenic
AATAATAAGTCATACAAACT+TGG 0.521678 4.2:-30422931 MS.gene045503:CDS
AGGATACATTAGGATGCAAA+GGG 0.521998 4.2:-30422115 MS.gene045503:CDS
GTGTTCCATCAGCAGTGGAT+TGG 0.522985 4.2:-30422792 MS.gene045503:CDS
GAGCAATGGATGAGTCACTA+TGG 0.524213 4.2:-30423045 MS.gene045503:CDS
TTGGTTGGTTAAGAATTCAT+GGG 0.526242 4.2:-30422157 MS.gene045503:CDS
GACACTTACCACATTGACCT+TGG 0.526963 4.2:+30422737 None:intergenic
AGTTTGACACTTAAGCAGTA+GGG 0.532124 4.2:+30422043 None:intergenic
ATTGAAGCTTCCAACAATGC+TGG 0.532605 4.2:-30422955 MS.gene045503:CDS
TCTGCTGTTGCGGCAACCGA+AGG 0.538022 4.2:-30422606 MS.gene045503:CDS
TAATTGATTATTGGCAGCCA+TGG 0.542700 4.2:+30423167 None:intergenic
CCGTAGCCATTGATGCTAGT+GGG 0.545236 4.2:-30422290 MS.gene045503:CDS
TCCGTAGCCATTGATGCTAG+TGG 0.552719 4.2:-30422291 MS.gene045503:CDS
ACTGCCGTTGGTTATGGTGT+TGG 0.553487 4.2:-30422198 MS.gene045503:CDS
GTAATGATGGAACCAAGTAT+TGG 0.556240 4.2:-30422176 MS.gene045503:CDS
TGTCCCTGCCAACAATGAGC+AGG 0.557594 4.2:-30422346 MS.gene045503:CDS
TTTCTTTGAATATCCTAAGA+CGG 0.559084 4.2:+30422989 None:intergenic
GAAGCTCAATACCCCTATCA+AGG 0.559311 4.2:-30422435 MS.gene045503:CDS
AAGGATACATTAGGATGCAA+AGG 0.562755 4.2:-30422116 MS.gene045503:CDS
TGATGGAACCAAGTATTGGT+TGG 0.568906 4.2:-30422172 MS.gene045503:CDS
ATGGGGAACTGATTGGGGTG+AGG 0.569509 4.2:-30422139 MS.gene045503:CDS
AGGGGAGTCGATGCTGCTGA+AGG 0.575211 4.2:-30422096 MS.gene045503:CDS
GGTGTAGATCAAGGTTGTGA+GGG 0.575537 4.2:-30422513 MS.gene045503:CDS
AATTCATGGGGAACTGATTG+GGG 0.577168 4.2:-30422144 MS.gene045503:CDS
TACCCCTATCAAGGTGTGGA+CGG 0.577316 4.2:-30422426 MS.gene045503:CDS
GAATTCATAAGTTGAGTACT+GGG 0.579056 4.2:-30422584 MS.gene045503:CDS
CCCAGTAATGGTAGCAGCAG+GGG 0.583241 4.2:+30422377 None:intergenic
CCCACTAGCATCAATGGCTA+CGG 0.584519 4.2:+30422290 None:intergenic
AGGACTTTGTGGCATTGCAA+TGG 0.587668 4.2:-30422076 MS.gene045503:CDS
GACACAAAAGGTGTAGATCA+AGG 0.591093 4.2:-30422522 MS.gene045503:CDS
GTAGATCAAGGTTGTGAGGG+TGG 0.591695 4.2:-30422510 MS.gene045503:CDS
GACCATGGTGTCACTGCCGT+TGG 0.593896 4.2:-30422210 MS.gene045503:CDS
TTCCGTCCACACCTTGATAG+GGG 0.599701 4.2:+30422424 None:intergenic
ATGCCTGCTCATTGTTGGCA+GGG 0.601537 4.2:+30422343 None:intergenic
GCATCTAGAAACAAATTCAA+GGG 0.601852 4.2:-30422868 MS.gene045503:CDS
GACATCCTCATACCCAGTAA+TGG 0.602674 4.2:+30422365 None:intergenic
TGCAAGTGTTCCATCAGCAG+TGG 0.603452 4.2:-30422797 MS.gene045503:CDS
TGTACGAGAGACACGAGCAA+TGG 0.611237 4.2:-30423059 MS.gene045503:CDS
TGGTTCATGTGGAACTGAGT+TGG 0.612813 4.2:-30422232 MS.gene045503:CDS
AGGTGTAGATCAAGGTTGTG+AGG 0.615496 4.2:-30422514 MS.gene045503:CDS
TGGTTGGTTAAGAATTCATG+GGG 0.622660 4.2:-30422156 MS.gene045503:CDS
ACCCAGTAATGGTAGCAGCA+GGG 0.635691 4.2:+30422376 None:intergenic
GGTGTCTTTACTGGTTCATG+TGG 0.637602 4.2:-30422243 MS.gene045503:CDS
CTATGAACTCCTCATTGGTG+AGG 0.645140 4.2:+30422890 None:intergenic
AACCAACGGCAGTGACACCA+TGG 0.652654 4.2:+30422208 None:intergenic
ATTTGCAGACCTCACCAATG+AGG 0.664553 4.2:-30422899 MS.gene045503:CDS
GTGAAGAACCAAGGTCAATG+TGG 0.671761 4.2:-30422745 MS.gene045503:intron
ATTACCAACACCATAACCAA+CGG 0.678062 4.2:+30422194 None:intergenic
TGTGGAACTGAGTTGGACCA+TGG 0.679648 4.2:-30422225 MS.gene045503:CDS
TCAATACCCCTATCAAGGTG+TGG 0.700771 4.2:-30422430 MS.gene045503:CDS
GTAACACCAGTGAAGAACCA+AGG 0.718576 4.2:-30422754 MS.gene045503:CDS
GGATACATTAGGATGCAAAG+GGG 0.764149 4.2:-30422114 MS.gene045503:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTAATTTACCTATTGT+AGG - chr4.2:30422573-30422592 MS.gene045503:CDS 15.0%
!! AAATTCATCATTCAAAATCA+TGG - chr4.2:30422750-30422769 MS.gene045503:CDS 20.0%
!! AATAATAAGTCATACAAACT+TGG - chr4.2:30422287-30422306 MS.gene045503:CDS 20.0%
!! ATTAAATCATAGCATTTCAT+TGG - chr4.2:30422070-30422089 MS.gene045503:CDS 20.0%
!! GCTATGATTTAATTGATTAT+TGG + chr4.2:30422063-30422082 None:intergenic 20.0%
! TTAGATCACATAGTATGTAA+TGG + chr4.2:30422527-30422546 None:intergenic 25.0%
! TTTCTTTGAATATCCTAAGA+CGG + chr4.2:30422232-30422251 None:intergenic 25.0%
ACTCAACTTATGAATTCCTT+CGG + chr4.2:30422631-30422650 None:intergenic 30.0%
AGCATCTAGAAACAAATTCA+AGG - chr4.2:30422349-30422368 MS.gene045503:CDS 30.0%
ATTCTTAACCAACCAATACT+TGG + chr4.2:30423057-30423076 None:intergenic 30.0%
CTATAAGGATTCTCAAGAAA+GGG - chr4.2:30422202-30422221 MS.gene045503:CDS 30.0%
GCATCTAGAAACAAATTCAA+GGG - chr4.2:30422350-30422369 MS.gene045503:CDS 30.0%
TCTATAAGGATTCTCAAGAA+AGG - chr4.2:30422201-30422220 MS.gene045503:CDS 30.0%
! ATTGGTTGGTTAAGAATTCA+TGG - chr4.2:30423060-30423079 MS.gene045503:CDS 30.0%
! GAATTCATAAGTTGAGTACT+GGG - chr4.2:30422634-30422653 MS.gene045503:intron 30.0%
! GACTTTCAGTTTTACAAAAG+TGG - chr4.2:30422954-30422973 MS.gene045503:CDS 30.0%
! TTGGTTGGTTAAGAATTCAT+GGG - chr4.2:30423061-30423080 MS.gene045503:CDS 30.0%
!! ATTTGAAAGCATCATCCATA+AGG + chr4.2:30422734-30422753 None:intergenic 30.0%
AAGGATACATTAGGATGCAA+AGG - chr4.2:30423102-30423121 MS.gene045503:CDS 35.0%
ACCTATTGTAGGATGTTGTT+GGG - chr4.2:30422584-30422603 MS.gene045503:CDS 35.0%
AGGATACATTAGGATGCAAA+GGG - chr4.2:30423103-30423122 MS.gene045503:CDS 35.0%
AGTAGTTGTCTTTGTGATTG+AGG + chr4.2:30422387-30422406 None:intergenic 35.0%
ATTACCAACACCATAACCAA+CGG + chr4.2:30423027-30423046 None:intergenic 35.0%
CTTATTATTGCCAGCATTGT+TGG + chr4.2:30422276-30422295 None:intergenic 35.0%
GGAACATGCAATGCTAATAA+AGG - chr4.2:30422813-30422832 MS.gene045503:CDS 35.0%
TACAAAAGTGGTGTCTTTAC+TGG - chr4.2:30422966-30422985 MS.gene045503:CDS 35.0%
TACCTATTGTAGGATGTTGT+TGG - chr4.2:30422583-30422602 MS.gene045503:CDS 35.0%
TCACTATGGCAAAGTCTATA+AGG - chr4.2:30422187-30422206 MS.gene045503:CDS 35.0%
TCATAGCATTTCATTGGCAT+TGG - chr4.2:30422076-30422095 MS.gene045503:CDS 35.0%
! GGAATTCATAAGTTGAGTAC+TGG - chr4.2:30422633-30422652 MS.gene045503:intron 35.0%
! GTAATGATGGAACCAAGTAT+TGG - chr4.2:30423042-30423061 MS.gene045503:CDS 35.0%
! TGGTTGGTTAAGAATTCATG+GGG - chr4.2:30423062-30423081 MS.gene045503:CDS 35.0%
!!! ATTGGCATTGGTTTTATGCT+TGG - chr4.2:30422088-30422107 MS.gene045503:CDS 35.0%
!!! TTGGCATTGGTTTTATGCTT+GGG - chr4.2:30422089-30422108 MS.gene045503:CDS 35.0%
AATTCATGGGGAACTGATTG+GGG - chr4.2:30423074-30423093 MS.gene045503:CDS 40.0%
AGAATTCATGGGGAACTGAT+TGG - chr4.2:30423072-30423091 MS.gene045503:CDS 40.0%
AGATGCTATGAACTCCTCAT+TGG + chr4.2:30422336-30422355 None:intergenic 40.0%
ATTGAAGCTTCCAACAATGC+TGG - chr4.2:30422263-30422282 MS.gene045503:CDS 40.0%
GAATTCATGGGGAACTGATT+GGG - chr4.2:30423073-30423092 MS.gene045503:CDS 40.0%
GGATACATTAGGATGCAAAG+GGG - chr4.2:30423104-30423123 MS.gene045503:CDS 40.0%
! CTTGTTGATTGCGACACAAA+AGG - chr4.2:30422684-30422703 MS.gene045503:intron 40.0%
! GACACAAAAGGTGTAGATCA+AGG - chr4.2:30422696-30422715 MS.gene045503:intron 40.0%
! TGGGAAGTTGGTCTCTTTAT+CGG - chr4.2:30422653-30422672 MS.gene045503:intron 40.0%
!! GGTTATGGTGTTGGTAATGA+TGG - chr4.2:30423029-30423048 MS.gene045503:CDS 40.0%
!! GGTTTTATGCTTGGGATTGT+TGG - chr4.2:30422097-30422116 MS.gene045503:CDS 40.0%
!! TAAGTTGAGTACTGGGAAGT+TGG - chr4.2:30422641-30422660 MS.gene045503:intron 40.0%
!! TGATGGAACCAAGTATTGGT+TGG - chr4.2:30423046-30423065 MS.gene045503:CDS 40.0%
AAGAAAGGGAGAACCGTCTT+AGG - chr4.2:30422216-30422235 MS.gene045503:CDS 45.0%
AGGACTTTGTGGCATTGCAA+TGG - chr4.2:30423142-30423161 MS.gene045503:CDS 45.0%
AGGTGTAGATCAAGGTTGTG+AGG - chr4.2:30422704-30422723 MS.gene045503:intron 45.0%
ATTTGCAGACCTCACCAATG+AGG - chr4.2:30422319-30422338 MS.gene045503:CDS 45.0%
CTATGAACTCCTCATTGGTG+AGG + chr4.2:30422331-30422350 None:intergenic 45.0%
GAAGCTCAATACCCCTATCA+AGG - chr4.2:30422783-30422802 MS.gene045503:CDS 45.0%
GACACTTACCACATTGACCT+TGG + chr4.2:30422484-30422503 None:intergenic 45.0%
GACATCCTCATACCCAGTAA+TGG + chr4.2:30422856-30422875 None:intergenic 45.0%
GAGCAATGGATGAGTCACTA+TGG - chr4.2:30422173-30422192 MS.gene045503:CDS 45.0%
GCATCAATGGCTACGGAAAT+TGG + chr4.2:30422924-30422943 None:intergenic 45.0%
GCCCAACAACATCCTACAAT+AGG + chr4.2:30422588-30422607 None:intergenic 45.0%
GGTGTAGATCAAGGTTGTGA+GGG - chr4.2:30422705-30422724 MS.gene045503:intron 45.0%
GTAACACCAGTGAAGAACCA+AGG - chr4.2:30422464-30422483 MS.gene045503:CDS 45.0%
GTGAAGAACCAAGGTCAATG+TGG - chr4.2:30422473-30422492 MS.gene045503:CDS 45.0%
TCAATACCCCTATCAAGGTG+TGG - chr4.2:30422788-30422807 MS.gene045503:CDS 45.0%
TCTCGTACATGGAGTCATCT+TGG + chr4.2:30422148-30422167 None:intergenic 45.0%
TGCTACCATTACTGGGTATG+AGG - chr4.2:30422848-30422867 MS.gene045503:CDS 45.0%
TGGTTCATGTGGAACTGAGT+TGG - chr4.2:30422986-30423005 MS.gene045503:CDS 45.0%
TTGCAATGCCTGCTCATTGT+TGG + chr4.2:30422883-30422902 None:intergenic 45.0%
TTTCTCCAATCCACTGCTGA+TGG + chr4.2:30422434-30422453 None:intergenic 45.0%
! CATTGACCTTGGTTCTTCAC+TGG + chr4.2:30422473-30422492 None:intergenic 45.0%
! TTGGGCATTTTCTGCTGTTG+CGG - chr4.2:30422602-30422621 MS.gene045503:CDS 45.0%
!! GCAGTGGATTGGAGAAAGAA+AGG - chr4.2:30422437-30422456 MS.gene045503:CDS 45.0%
!! GGTGTCTTTACTGGTTCATG+TGG - chr4.2:30422975-30422994 MS.gene045503:CDS 45.0%
AATGCCTGCTCATTGTTGGC+AGG + chr4.2:30422879-30422898 None:intergenic 50.0%
ACCCAGTAATGGTAGCAGCA+GGG + chr4.2:30422845-30422864 None:intergenic 50.0%
ATGCCTGCTCATTGTTGGCA+GGG + chr4.2:30422878-30422897 None:intergenic 50.0%
CCCACTAGCATCAATGGCTA+CGG + chr4.2:30422931-30422950 None:intergenic 50.0%
CCGTAGCCATTGATGCTAGT+GGG - chr4.2:30422928-30422947 MS.gene045503:CDS 50.0%
GATGCTGCTGAAGGACTTTG+TGG - chr4.2:30423131-30423150 MS.gene045503:CDS 50.0%
GGGGTGAGGAAGGATACATT+AGG - chr4.2:30423093-30423112 MS.gene045503:CDS 50.0%
GTAGATCAAGGTTGTGAGGG+TGG - chr4.2:30422708-30422727 MS.gene045503:intron 50.0%
GTCAGACCCACTAGCATCAA+TGG + chr4.2:30422937-30422956 None:intergenic 50.0%
GTGTTCCATCAGCAGTGGAT+TGG - chr4.2:30422426-30422445 MS.gene045503:CDS 50.0%
GTTCCGTCCACACCTTGATA+GGG + chr4.2:30422798-30422817 None:intergenic 50.0%
TACCCAGTAATGGTAGCAGC+AGG + chr4.2:30422846-30422865 None:intergenic 50.0%
TACCCCTATCAAGGTGTGGA+CGG - chr4.2:30422792-30422811 MS.gene045503:CDS 50.0%
TCCGTAGCCATTGATGCTAG+TGG - chr4.2:30422927-30422946 MS.gene045503:CDS 50.0%
TGCAAGTGTTCCATCAGCAG+TGG - chr4.2:30422421-30422440 MS.gene045503:CDS 50.0%
TGTACGAGAGACACGAGCAA+TGG - chr4.2:30422159-30422178 MS.gene045503:CDS 50.0%
TGTTCCGTCCACACCTTGAT+AGG + chr4.2:30422799-30422818 None:intergenic 50.0%
TTCCGTCCACACCTTGATAG+GGG + chr4.2:30422797-30422816 None:intergenic 50.0%
TTGCTCGTGTCTCTCGTACA+TGG + chr4.2:30422159-30422178 None:intergenic 50.0%
! ACTGCCGTTGGTTATGGTGT+TGG - chr4.2:30423020-30423039 MS.gene045503:CDS 50.0%
! GCAGGCATTGCAAAAAGCTG+TGG - chr4.2:30422890-30422909 MS.gene045503:CDS 50.0%
! TGTGGAACTGAGTTGGACCA+TGG - chr4.2:30422993-30423012 MS.gene045503:CDS 50.0%
AACCAACGGCAGTGACACCA+TGG + chr4.2:30423013-30423032 None:intergenic 55.0%
ATGGGGAACTGATTGGGGTG+AGG - chr4.2:30423079-30423098 MS.gene045503:CDS 55.0%
CCCAGTAATGGTAGCAGCAG+GGG + chr4.2:30422844-30422863 None:intergenic 55.0%
CCCCTGCTGCTACCATTACT+GGG - chr4.2:30422841-30422860 MS.gene045503:CDS 55.0%
GGAACTGATTGGGGTGAGGA+AGG - chr4.2:30423083-30423102 MS.gene045503:CDS 55.0%
TGTCCCTGCCAACAATGAGC+AGG - chr4.2:30422872-30422891 MS.gene045503:CDS 55.0%
! AGGTTGTGAGGGTGGCCTTA+TGG - chr4.2:30422716-30422735 MS.gene045503:intron 55.0%
!! GGTGTCACTGCCGTTGGTTA+TGG - chr4.2:30423014-30423033 MS.gene045503:CDS 55.0%
AGGGGAGTCGATGCTGCTGA+AGG - chr4.2:30423122-30423141 MS.gene045503:CDS 60.0%
GCCCCTGCTGCTACCATTAC+TGG - chr4.2:30422840-30422859 MS.gene045503:CDS 60.0%
TCTGCTGTTGCGGCAACCGA+AGG - chr4.2:30422612-30422631 MS.gene045503:CDS 60.0%
!! GACCATGGTGTCACTGCCGT+TGG - chr4.2:30423008-30423027 MS.gene045503:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 30422053 30423187 30422053 ID=MS.gene045503
chr4.2 mRNA 30422053 30423187 30422053 ID=MS.gene045503.t1;Parent=MS.gene045503
chr4.2 exon 30422746 30423187 30422746 ID=MS.gene045503.t1.exon1;Parent=MS.gene045503.t1
chr4.2 CDS 30422746 30423187 30422746 ID=cds.MS.gene045503.t1;Parent=MS.gene045503.t1
chr4.2 exon 30422053 30422645 30422053 ID=MS.gene045503.t1.exon2;Parent=MS.gene045503.t1
chr4.2 CDS 30422053 30422645 30422053 ID=cds.MS.gene045503.t1;Parent=MS.gene045503.t1
Gene Sequence

>MS.gene045503

ATGGCTGCCAATAATCAATTAAATCATAGCATTTCATTGGCATTGGTTTTATGCTTGGGATTGTTGGCTATTCAAGTCACTTCTCGTACTCTCCAAGATGACTCCATGTACGAGAGACACGAGCAATGGATGAGTCACTATGGCAAAGTCTATAAGGATTCTCAAGAAAGGGAGAACCGTCTTAGGATATTCAAAGAAAATGTGAATTACATTGAAGCTTCCAACAATGCTGGCAATAATAAGTCATACAAACTTGGCATTAATCAATTTGCAGACCTCACCAATGAGGAGTTCATAGCATCTAGAAACAAATTCAAGGGTCATATGTGCTCCTCAATCACAAAGACAACTACTTTTATGTATGAAAATGCAAGTGTTCCATCAGCAGTGGATTGGAGAAAGAAAGGAGCAGTAACACCAGTGAAGAACCAAGGTCAATGTGGTAAGTGTCTATCTAATATGTAATGCATTCCATTACATACTATGTGATCTAATATTGAATTGTCATTAAACTCTAGTTAATTTTAATTTACCTATTGTAGGATGTTGTTGGGCATTTTCTGCTGTTGCGGCAACCGAAGGAATTCATAAGTTGAGTACTGGGAAGTTGGTCTCTTTATCGGAACAAGAACTTGTTGATTGCGACACAAAAGGTGTAGATCAAGGTTGTGAGGGTGGCCTTATGGATGATGCTTTCAAATTCATCATTCAAAATCATGGACTCAACACTGAAGCTCAATACCCCTATCAAGGTGTGGACGGAACATGCAATGCTAATAAAGGTTCAGCCCCTGCTGCTACCATTACTGGGTATGAGGATGTCCCTGCCAACAATGAGCAGGCATTGCAAAAAGCTGTGGCAAATCAACCAATTTCCGTAGCCATTGATGCTAGTGGGTCTGACTTTCAGTTTTACAAAAGTGGTGTCTTTACTGGTTCATGTGGAACTGAGTTGGACCATGGTGTCACTGCCGTTGGTTATGGTGTTGGTAATGATGGAACCAAGTATTGGTTGGTTAAGAATTCATGGGGAACTGATTGGGGTGAGGAAGGATACATTAGGATGCAAAGGGGAGTCGATGCTGCTGAAGGACTTTGTGGCATTGCAATGGAAGCATCTTACCCTACTGCTTAA
Protein sequence

>MS.gene045503.t1

MAANNQLNHSISLALVLCLGLLAIQVTSRTLQDDSMYERHEQWMSHYGKVYKDSQERENRLRIFKENVNYIEASNNAGNNKSYKLGINQFADLTNEEFIASRNKFKGHMCSSITKTTTFMYENASVPSAVDWRKKGAVTPVKNQGQCGCCWAFSAVAATEGIHKLSTGKLVSLSEQELVDCDTKGVDQGCEGGLMDDAFKFIIQNHGLNTEAQYPYQGVDGTCNANKGSAPAATITGYEDVPANNEQALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGVGNDGTKYWLVKNSWGTDWGEEGYIRMQRGVDAAEGLCGIAMEASYPTA