AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26406


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26406.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene26406.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene26406.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene26406.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene26406.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene26406.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene26406.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene26406.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene26406.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene26406.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene26406.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene26406.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene26406.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene26406.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene26406.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene26406.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene26406.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene26406.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene26406.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene26406.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene26406.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene26406.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene26406.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene26406.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene26406.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene26406.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene26406.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene26406.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene26406.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene26406.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene26406.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene26406.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene26406.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26406.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene26406.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene26406.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene26406.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene26406.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene26406.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene26406.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene26406.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene26406.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene26406.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene26406.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene26406.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene26406.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene26406.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene26406.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene26406.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene26406.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene26406.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene26406.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene26406.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene26406.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene26406.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 50 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAAACCTCACCGTTTC+AGG 0.224781 4.1:-22158458 MS.gene26406:CDS
TCACGGAGAGCGACGGTTCC+TGG 0.319730 4.1:+22158435 None:intergenic
TGAGGAGCTCAGTGCTCTTC+TGG 0.339681 4.1:+22158400 None:intergenic
GTGCCGACACGGCGGAGCTC+TGG 0.383446 4.1:+22158310 None:intergenic
TTCTTCACTCCGCCGGTGGC+CGG 0.393192 4.1:+22158474 None:intergenic
TTTGTGGCGAGTTGCTTCCT+TGG 0.400731 4.1:+22158516 None:intergenic
GGAAGGGGAGTTTGCGGATG+AGG 0.429699 4.1:+22158382 None:intergenic
TTATCTCGTCGGTCTCTTTG+AGG 0.447224 4.1:-22158268 MS.gene26406:CDS
CTTCCTTGGAGCTTTGCCAC+CGG 0.456245 4.1:+22158530 None:intergenic
CGCAAATCTGCTCCGGCCAC+CGG 0.457456 4.1:-22158486 MS.gene26406:CDS
CGCTTCTTGCAGTGCCGACA+CGG 0.458211 4.1:+22158299 None:intergenic
AGTGACACGCTTAGCATGAA+TGG 0.474744 4.1:+22158230 None:intergenic
CCTTGGAGCTTTGCCACCGG+TGG 0.488901 4.1:+22158533 None:intergenic
GGCACTGCAAGAAGCGGCCG+AGG 0.489550 4.1:-22158292 MS.gene26406:CDS
CTAGCGAGCTGGATATCCTT+GGG 0.491052 4.1:+22158201 None:intergenic
CCTAGCGAGCTGGATATCCT+TGG 0.497532 4.1:+22158200 None:intergenic
CGACGGTTCCTGGCCTGAAA+CGG 0.503357 4.1:+22158445 None:intergenic
GGATTTGCGAGCGGTTTGCT+TGG 0.506530 4.1:+22158554 None:intergenic
AAACCTCACCGTTTCAGGCC+AGG 0.512079 4.1:-22158453 MS.gene26406:CDS
CACGGATACGCCTAGCGAGC+TGG 0.513784 4.1:+22158190 None:intergenic
GACGCTGGAAGGGGAGTTTG+CGG 0.521996 4.1:+22158376 None:intergenic
TTTCCAGAGCTCCGCCGTGT+CGG 0.522343 4.1:-22158313 MS.gene26406:CDS
ACACGGCGGAGCTCTGGAAA+CGG 0.525377 4.1:+22158316 None:intergenic
AGCCGCTCGCAAATCTGCTC+CGG 0.526992 4.1:-22158493 MS.gene26406:CDS
CACGGACAAGACGCTGGAAG+GGG 0.529109 4.1:+22158367 None:intergenic
CCAAGGATATCCAGCTCGCT+AGG 0.536369 4.1:-22158200 MS.gene26406:CDS
CTCACGGACAAGACGCTGGA+AGG 0.539901 4.1:+22158365 None:intergenic
GTGAGGTTTCTTCACTCCGC+CGG 0.553823 4.1:+22158467 None:intergenic
TGAAATCCTGAGCGATCTCA+CGG 0.570370 4.1:+22158349 None:intergenic
TCTGGTACTTGCGGATCTCA+CGG 0.571857 4.1:+22158418 None:intergenic
CCACCGGTGGCAAAGCTCCA+AGG 0.572773 4.1:-22158533 MS.gene26406:CDS
CAGTGCTCTTCTGGTACTTG+CGG 0.579006 4.1:+22158409 None:intergenic
TCACGGACAAGACGCTGGAA+GGG 0.583031 4.1:+22158366 None:intergenic
AAATCTGCTCCGGCCACCGG+CGG 0.584127 4.1:-22158483 MS.gene26406:CDS
CGATCTCACGGACAAGACGC+TGG 0.585935 4.1:+22158361 None:intergenic
AGGTTTCTTCACTCCGCCGG+TGG 0.590490 4.1:+22158470 None:intergenic
CGTGTCGGCACTGCAAGAAG+CGG 0.596423 4.1:-22158298 MS.gene26406:CDS
TCTTGTCCGTGAGATCGCTC+AGG 0.603921 4.1:-22158355 MS.gene26406:CDS
GCGGCCGAGGCTTATCTCGT+CGG 0.608232 4.1:-22158279 MS.gene26406:CDS
CAAACCGCTCGCAAATCCAC+CGG 0.613586 4.1:-22158549 MS.gene26406:CDS
GCGTGTCACTATCATGCCCA+AGG 0.632623 4.1:-22158217 MS.gene26406:CDS
ACCGCTCGCAAATCCACCGG+TGG 0.632751 4.1:-22158546 MS.gene26406:CDS
AAGATCAAGCACGCTCGCCA+CGG 0.639365 4.1:+22158172 None:intergenic
GGCCGGAGCAGATTTGCGAG+CGG 0.640066 4.1:+22158491 None:intergenic
GCCACCGGTGGATTTGCGAG+CGG 0.642552 4.1:+22158545 None:intergenic
GAGACCGACGAGATAAGCCT+CGG 0.653052 4.1:+22158275 None:intergenic
GCGGATCTCACGGAGAGCGA+CGG 0.662183 4.1:+22158428 None:intergenic
GTTCCTGGCCTGAAACGGTG+AGG 0.663103 4.1:+22158450 None:intergenic
CAGCTCGCTAGGCGTATCCG+TGG 0.667707 4.1:-22158189 MS.gene26406:CDS
TTCTTGCAGTGCCGACACGG+CGG 0.739278 4.1:+22158302 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGTGACACGCTTAGCATGAA+TGG + chr4.1:22158513-22158532 None:intergenic 45.0%
TGAAATCCTGAGCGATCTCA+CGG + chr4.1:22158394-22158413 None:intergenic 45.0%
TGAAGAAACCTCACCGTTTC+AGG - chr4.1:22158282-22158301 MS.gene26406:CDS 45.0%
TTATCTCGTCGGTCTCTTTG+AGG - chr4.1:22158472-22158491 MS.gene26406:CDS 45.0%
CAGTGCTCTTCTGGTACTTG+CGG + chr4.1:22158334-22158353 None:intergenic 50.0%
CTAGCGAGCTGGATATCCTT+GGG + chr4.1:22158542-22158561 None:intergenic 50.0%
TCTGGTACTTGCGGATCTCA+CGG + chr4.1:22158325-22158344 None:intergenic 50.0%
TTTGTGGCGAGTTGCTTCCT+TGG + chr4.1:22158227-22158246 None:intergenic 50.0%
! AGATTTGCGAGCGGCTTTTG+TGG + chr4.1:22158243-22158262 None:intergenic 50.0%
AAACCTCACCGTTTCAGGCC+AGG - chr4.1:22158287-22158306 MS.gene26406:CDS 55.0%
CAAACCGCTCGCAAATCCAC+CGG - chr4.1:22158191-22158210 MS.gene26406:CDS 55.0%
CCAAGGATATCCAGCTCGCT+AGG - chr4.1:22158540-22158559 MS.gene26406:CDS 55.0%
CCTAGCGAGCTGGATATCCT+TGG + chr4.1:22158543-22158562 None:intergenic 55.0%
CTTCCTTGGAGCTTTGCCAC+CGG + chr4.1:22158213-22158232 None:intergenic 55.0%
GAGACCGACGAGATAAGCCT+CGG + chr4.1:22158468-22158487 None:intergenic 55.0%
GTGAGGTTTCTTCACTCCGC+CGG + chr4.1:22158276-22158295 None:intergenic 55.0%
TCACGGACAAGACGCTGGAA+GGG + chr4.1:22158377-22158396 None:intergenic 55.0%
TCTTGTCCGTGAGATCGCTC+AGG - chr4.1:22158385-22158404 MS.gene26406:CDS 55.0%
TGAGGAGCTCAGTGCTCTTC+TGG + chr4.1:22158343-22158362 None:intergenic 55.0%
! GCGTGTCACTATCATGCCCA+AGG - chr4.1:22158523-22158542 MS.gene26406:CDS 55.0%
! GGATTTGCGAGCGGTTTGCT+TGG + chr4.1:22158189-22158208 None:intergenic 55.0%
AGCCGCTCGCAAATCTGCTC+CGG - chr4.1:22158247-22158266 MS.gene26406:CDS 60.0%
AGGTTTCTTCACTCCGCCGG+TGG + chr4.1:22158273-22158292 None:intergenic 60.0%
CACGGACAAGACGCTGGAAG+GGG + chr4.1:22158376-22158395 None:intergenic 60.0%
CGACGGTTCCTGGCCTGAAA+CGG + chr4.1:22158298-22158317 None:intergenic 60.0%
CGATCTCACGGACAAGACGC+TGG + chr4.1:22158382-22158401 None:intergenic 60.0%
CTCACGGACAAGACGCTGGA+AGG + chr4.1:22158378-22158397 None:intergenic 60.0%
GACGCTGGAAGGGGAGTTTG+CGG + chr4.1:22158367-22158386 None:intergenic 60.0%
GGAAGGGGAGTTTGCGGATG+AGG + chr4.1:22158361-22158380 None:intergenic 60.0%
GTTCCTGGCCTGAAACGGTG+AGG + chr4.1:22158293-22158312 None:intergenic 60.0%
TTTCCAGAGCTCCGCCGTGT+CGG - chr4.1:22158427-22158446 MS.gene26406:CDS 60.0%
! CGTGTCGGCACTGCAAGAAG+CGG - chr4.1:22158442-22158461 MS.gene26406:CDS 60.0%
!! ACACGGCGGAGCTCTGGAAA+CGG + chr4.1:22158427-22158446 None:intergenic 60.0%
!! CGCTTCTTGCAGTGCCGACA+CGG + chr4.1:22158444-22158463 None:intergenic 60.0%
!! TTCTTGCAGTGCCGACACGG+CGG + chr4.1:22158441-22158460 None:intergenic 60.0%
AAATCTGCTCCGGCCACCGG+CGG - chr4.1:22158257-22158276 MS.gene26406:CDS 65.0%
ACCGCTCGCAAATCCACCGG+TGG - chr4.1:22158194-22158213 MS.gene26406:CDS 65.0%
CACGGATACGCCTAGCGAGC+TGG + chr4.1:22158553-22158572 None:intergenic 65.0%
CAGCTCGCTAGGCGTATCCG+TGG - chr4.1:22158551-22158570 MS.gene26406:CDS 65.0%
CCACCGGTGGCAAAGCTCCA+AGG - chr4.1:22158207-22158226 MS.gene26406:CDS 65.0%
CCTTGGAGCTTTGCCACCGG+TGG + chr4.1:22158210-22158229 None:intergenic 65.0%
CGCAAATCTGCTCCGGCCAC+CGG - chr4.1:22158254-22158273 MS.gene26406:CDS 65.0%
GCGGATCTCACGGAGAGCGA+CGG + chr4.1:22158315-22158334 None:intergenic 65.0%
GCGGCCGAGGCTTATCTCGT+CGG - chr4.1:22158461-22158480 MS.gene26406:CDS 65.0%
GGCCGGAGCAGATTTGCGAG+CGG + chr4.1:22158252-22158271 None:intergenic 65.0%
TCACGGAGAGCGACGGTTCC+TGG + chr4.1:22158308-22158327 None:intergenic 65.0%
TTCTTCACTCCGCCGGTGGC+CGG + chr4.1:22158269-22158288 None:intergenic 65.0%
! GCCACCGGTGGATTTGCGAG+CGG + chr4.1:22158198-22158217 None:intergenic 65.0%
GGCACTGCAAGAAGCGGCCG+AGG - chr4.1:22158448-22158467 MS.gene26406:CDS 70.0%
! GTGCCGACACGGCGGAGCTC+TGG + chr4.1:22158433-22158452 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 22158176 22158586 22158176 ID=MS.gene26406
chr4.1 mRNA 22158176 22158586 22158176 ID=MS.gene26406.t1;Parent=MS.gene26406
chr4.1 exon 22158176 22158586 22158176 ID=MS.gene26406.t1.exon1;Parent=MS.gene26406.t1
chr4.1 CDS 22158176 22158586 22158176 ID=cds.MS.gene26406.t1;Parent=MS.gene26406.t1
Gene Sequence

>MS.gene26406

ATGGCACGTACCAAGCAAACCGCTCGCAAATCCACCGGTGGCAAAGCTCCAAGGAAGCAACTCGCCACAAAAGCCGCTCGCAAATCTGCTCCGGCCACCGGCGGAGTGAAGAAACCTCACCGTTTCAGGCCAGGAACCGTCGCTCTCCGTGAGATCCGCAAGTACCAGAAGAGCACTGAGCTCCTCATCCGCAAACTCCCCTTCCAGCGTCTTGTCCGTGAGATCGCTCAGGATTTCAAGACTGATCTCCGTTTCCAGAGCTCCGCCGTGTCGGCACTGCAAGAAGCGGCCGAGGCTTATCTCGTCGGTCTCTTTGAGGATACTAATCTCTGCGCCATTCATGCTAAGCGTGTCACTATCATGCCCAAGGATATCCAGCTCGCTAGGCGTATCCGTGGCGAGCGTGCTTGA

Protein sequence

>MS.gene26406.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA