AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70263


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70263.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene70263.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene70263.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene70263.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene70263.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene70263.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene70263.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene70263.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene70263.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene70263.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene70263.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene70263.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene70263.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene70263.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene70263.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene70263.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene70263.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene70263.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene70263.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene70263.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene70263.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene70263.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene70263.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene70263.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene70263.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene70263.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene70263.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene70263.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene70263.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene70263.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene70263.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene70263.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene70263.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70263.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene70263.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene70263.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene70263.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene70263.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene70263.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene70263.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene70263.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene70263.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene70263.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene70263.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene70263.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene70263.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene70263.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene70263.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene70263.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene70263.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene70263.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene70263.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene70263.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene70263.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene70263.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 38 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTTGCCGGAGCTGATTTC+CGG 0.223115 4.1:+8876130 None:intergenic
CTTTGGTTGCCAGTTGCTTC+CGG 0.239809 4.1:+8876157 None:intergenic
TTCTTCACTCCTCCGGTTGC+CGG 0.280449 4.1:+8876117 None:intergenic
TTTGGTTGCCAGTTGCTTCC+GGG 0.293951 4.1:+8876158 None:intergenic
AGCTGATTTCCGGGCGGCTT+TGG 0.313671 4.1:+8876140 None:intergenic
TCTCTCAAAGCAACTGTTCC+AGG 0.333816 4.1:+8876078 None:intergenic
ATTCCAGAGCAGTGCTGTTT+CGG 0.344458 4.1:-8875956 MS.gene70263:CDS
GGTTGCCGGAGCTGATTTCC+GGG 0.374321 4.1:+8876131 None:intergenic
CAAGGCTCCCCGGAAGCAAC+TGG 0.376550 4.1:-8876166 MS.gene70263:CDS
CGGAAATCAGCTCCGGCAAC+CGG 0.399036 4.1:-8876129 MS.gene70263:CDS
AGAAGCGGCTGAAGCTTACT+TGG 0.416086 4.1:-8875926 MS.gene70263:CDS
GAGCCGAAACAGCACTGCTC+TGG 0.422418 4.1:+8875953 None:intergenic
GACGTTGGAATGGAAGTTTA+CGG 0.458530 4.1:+8876019 None:intergenic
AGGCATGATTGTAACTCTCT+TGG 0.461766 4.1:+8875864 None:intergenic
CTTGCAAGCTGAATGTCCTT+AGG 0.462646 4.1:+8875844 None:intergenic
GTGTGGCTTCTTCACTCCTC+CGG 0.469362 4.1:+8876110 None:intergenic
CCACCGGCGGCAAGGCTCCC+CGG 0.480154 4.1:-8876176 MS.gene70263:CDS
TCGCAAGTCCACCGGCGGCA+AGG 0.488583 4.1:-8876184 MS.gene70263:CDS
AGCCGCCCGGAAATCAGCTC+CGG 0.490847 4.1:-8876136 MS.gene70263:CDS
TTGGTTGCCAGTTGCTTCCG+GGG 0.522282 4.1:+8876159 None:intergenic
AACTGGCAACCAAAGCCGCC+CGG 0.530994 4.1:-8876149 MS.gene70263:CDS
CTTCCGGGGAGCCTTGCCGC+CGG 0.543290 4.1:+8876173 None:intergenic
CCGGGGAGCCTTGCCGCCGG+TGG 0.548377 4.1:+8876176 None:intergenic
GGACTTGCGAGCAGTCTGCT+TGG 0.551577 4.1:+8876197 None:intergenic
CAGACTGCTCGCAAGTCCAC+CGG 0.562052 4.1:-8876192 MS.gene70263:CDS
GCGGCTGAAGCTTACTTGGT+CGG 0.565776 4.1:-8875922 MS.gene70263:CDS
GAGAGTTACAATCATGCCTA+AGG 0.573275 4.1:-8875860 MS.gene70263:CDS
AAGAAGAATCAGAGGAGAGA+GGG 0.573886 4.1:-8875824 MS.gene70263:CDS
TCACGAACAAGACGTTGGAA+TGG 0.598115 4.1:+8876009 None:intergenic
CAAGAAGAATCAGAGGAGAG+AGG 0.603703 4.1:-8875825 MS.gene70263:CDS
AAATCAGCTCCGGCAACCGG+AGG 0.609979 4.1:-8876126 MS.gene70263:CDS
CGATTTCACGAACAAGACGT+TGG 0.613448 4.1:+8876004 None:intergenic
CAGCTTGCAAGAAGAATCAG+AGG 0.621034 4.1:-8875832 MS.gene70263:CDS
ACTGCTCGCAAGTCCACCGG+CGG 0.647164 4.1:-8876189 MS.gene70263:CDS
AAGCCACACAGATTCAGACC+TGG 0.672238 4.1:-8876096 MS.gene70263:CDS
TGTTTCGGCTCTTCAAGAAG+CGG 0.672573 4.1:-8875941 MS.gene70263:CDS
GTTCCAGGTCTGAATCTGTG+TGG 0.688220 4.1:+8876093 None:intergenic
TGCCGGAGCTGATTTCCGGG+CGG 0.740409 4.1:+8876134 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGAAGAATCAGAGGAGAGA+GGG - chr4.1:8876202-8876221 MS.gene70263:CDS 40.0%
AGGCATGATTGTAACTCTCT+TGG + chr4.1:8876165-8876184 None:intergenic 40.0%
GACGTTGGAATGGAAGTTTA+CGG + chr4.1:8876010-8876029 None:intergenic 40.0%
GAGAGTTACAATCATGCCTA+AGG - chr4.1:8876166-8876185 MS.gene70263:CDS 40.0%
CAAGAAGAATCAGAGGAGAG+AGG - chr4.1:8876201-8876220 MS.gene70263:CDS 45.0%
CAGCTTGCAAGAAGAATCAG+AGG - chr4.1:8876194-8876213 MS.gene70263:CDS 45.0%
CGATTTCACGAACAAGACGT+TGG + chr4.1:8876025-8876044 None:intergenic 45.0%
CTTGCAAGCTGAATGTCCTT+AGG + chr4.1:8876185-8876204 None:intergenic 45.0%
TCACGAACAAGACGTTGGAA+TGG + chr4.1:8876020-8876039 None:intergenic 45.0%
TCTCTCAAAGCAACTGTTCC+AGG + chr4.1:8875951-8875970 None:intergenic 45.0%
TGTTTCGGCTCTTCAAGAAG+CGG - chr4.1:8876085-8876104 MS.gene70263:CDS 45.0%
!! ATTCCAGAGCAGTGCTGTTT+CGG - chr4.1:8876070-8876089 MS.gene70263:CDS 45.0%
AAGCCACACAGATTCAGACC+TGG - chr4.1:8875930-8875949 MS.gene70263:CDS 50.0%
AGAAGCGGCTGAAGCTTACT+TGG - chr4.1:8876100-8876119 MS.gene70263:CDS 50.0%
CTTTGGTTGCCAGTTGCTTC+CGG + chr4.1:8875872-8875891 None:intergenic 50.0%
GTTCCAGGTCTGAATCTGTG+TGG + chr4.1:8875936-8875955 None:intergenic 50.0%
TTTGGTTGCCAGTTGCTTCC+GGG + chr4.1:8875871-8875890 None:intergenic 50.0%
GCGGCTGAAGCTTACTTGGT+CGG - chr4.1:8876104-8876123 MS.gene70263:CDS 55.0%
GTGTGGCTTCTTCACTCCTC+CGG + chr4.1:8875919-8875938 None:intergenic 55.0%
TTCTTCACTCCTCCGGTTGC+CGG + chr4.1:8875912-8875931 None:intergenic 55.0%
TTGGTTGCCAGTTGCTTCCG+GGG + chr4.1:8875870-8875889 None:intergenic 55.0%
AAATCAGCTCCGGCAACCGG+AGG - chr4.1:8875900-8875919 MS.gene70263:CDS 60.0%
AACTGGCAACCAAAGCCGCC+CGG - chr4.1:8875877-8875896 MS.gene70263:CDS 60.0%
CAGACTGCTCGCAAGTCCAC+CGG - chr4.1:8875834-8875853 MS.gene70263:CDS 60.0%
CGGAAATCAGCTCCGGCAAC+CGG - chr4.1:8875897-8875916 MS.gene70263:CDS 60.0%
GAGCCGAAACAGCACTGCTC+TGG + chr4.1:8876076-8876095 None:intergenic 60.0%
GGACTTGCGAGCAGTCTGCT+TGG + chr4.1:8875832-8875851 None:intergenic 60.0%
! AGCTGATTTCCGGGCGGCTT+TGG + chr4.1:8875889-8875908 None:intergenic 60.0%
! CGGTTGCCGGAGCTGATTTC+CGG + chr4.1:8875899-8875918 None:intergenic 60.0%
! GGTTGCCGGAGCTGATTTCC+GGG + chr4.1:8875898-8875917 None:intergenic 60.0%
ACTGCTCGCAAGTCCACCGG+CGG - chr4.1:8875837-8875856 MS.gene70263:CDS 65.0%
AGCCGCCCGGAAATCAGCTC+CGG - chr4.1:8875890-8875909 MS.gene70263:CDS 65.0%
CAAGGCTCCCCGGAAGCAAC+TGG - chr4.1:8875860-8875879 MS.gene70263:CDS 65.0%
! TGCCGGAGCTGATTTCCGGG+CGG + chr4.1:8875895-8875914 None:intergenic 65.0%
TCGCAAGTCCACCGGCGGCA+AGG - chr4.1:8875842-8875861 MS.gene70263:CDS 70.0%
CTTCCGGGGAGCCTTGCCGC+CGG + chr4.1:8875856-8875875 None:intergenic 75.0%
! CCACCGGCGGCAAGGCTCCC+CGG - chr4.1:8875850-8875869 MS.gene70263:CDS 80.0%
! CCGGGGAGCCTTGCCGCCGG+TGG + chr4.1:8875853-8875872 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 8875819 8876229 8875819 ID=MS.gene70263
chr4.1 mRNA 8875819 8876229 8875819 ID=MS.gene70263.t1;Parent=MS.gene70263
chr4.1 exon 8875819 8876229 8875819 ID=MS.gene70263.t1.exon1;Parent=MS.gene70263.t1
chr4.1 CDS 8875819 8876229 8875819 ID=cds.MS.gene70263.t1;Parent=MS.gene70263.t1
Gene Sequence

>MS.gene70263

ATGGCACGTACCAAGCAGACTGCTCGCAAGTCCACCGGCGGCAAGGCTCCCCGGAAGCAACTGGCAACCAAAGCCGCCCGGAAATCAGCTCCGGCAACCGGAGGAGTGAAGAAGCCACACAGATTCAGACCTGGAACAGTTGCTTTGAGAGAGATTCGTAAGTATCAGAAGAGCACTGAGCTTCTCATCCGTAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCTCAAGATTTCAAGACAGATCTTAGATTCCAGAGCAGTGCTGTTTCGGCTCTTCAAGAAGCGGCTGAAGCTTACTTGGTCGGTCTCTTTGAAGATACTAATCTTTGTGCAATTCATGCCAAGAGAGTTACAATCATGCCTAAGGACATTCAGCTTGCAAGAAGAATCAGAGGAGAGAGGGCTTAG

Protein sequence

>MS.gene70263.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA