AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060218

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060218.t1 MTR_2g075830 95.015 341 17 0 1 341 1 341 0.0 659
MS.gene060218.t1 MTR_5g022560 74.709 344 84 2 1 341 1 344 0.0 540
MS.gene060218.t1 MTR_4g079770 75.504 347 77 6 1 341 1 345 0.0 538
MS.gene060218.t1 MTR_4g107930 74.203 345 85 3 1 341 1 345 0.0 535
MS.gene060218.t1 MTR_4g047610 74.783 345 81 4 1 341 1 343 0.0 518
MS.gene060218.t1 MTR_4g079470 76.074 326 71 5 21 341 22 345 0.0 514
MS.gene060218.t1 MTR_4g079800 74.540 326 76 5 21 341 22 345 0.0 507
MS.gene060218.t1 MTR_4g080700 63.608 316 112 3 27 341 27 340 3.38e-152 431
MS.gene060218.t1 MTR_4g080730 62.975 316 114 3 27 341 27 340 5.29e-150 426
MS.gene060218.t1 MTR_4g080360 54.896 337 144 4 10 341 9 342 4.29e-135 388
MS.gene060218.t1 MTR_2g083930 51.873 347 149 5 3 339 4 342 3.68e-126 365
MS.gene060218.t1 MTR_7g078600 55.591 313 128 5 31 339 31 336 2.74e-124 360
MS.gene060218.t1 MTR_4g104370 52.907 344 146 8 6 339 5 342 1.56e-123 359
MS.gene060218.t1 MTR_1g107550 54.103 329 142 6 18 339 28 354 5.52e-122 359
MS.gene060218.t1 MTR_3g075390 52.035 344 150 7 5 339 6 343 7.26e-122 355
MS.gene060218.t1 MTR_4g079440 55.375 307 134 3 33 339 40 343 8.51e-121 352
MS.gene060218.t1 MTR_7g079230 53.994 313 131 4 31 339 31 334 1.34e-119 348
MS.gene060218.t1 MTR_3g116080 55.096 314 128 6 33 340 41 347 7.53e-118 344
MS.gene060218.t1 MTR_2g090890 48.824 340 168 4 1 339 1 335 1.76e-116 340
MS.gene060218.t1 MTR_1g018840 49.718 354 160 8 1 339 10 360 3.03e-115 342
MS.gene060218.t1 MTR_1g018840 49.718 354 160 8 1 339 10 360 3.26e-115 342
MS.gene060218.t1 MTR_4g094918 49.558 339 160 6 10 340 6 341 1.39e-114 337
MS.gene060218.t1 MTR_3g102220 49.857 349 153 11 10 339 15 360 3.29e-109 327
MS.gene060218.t1 MTR_3g112430 51.447 311 135 4 40 340 37 341 1.63e-107 321
MS.gene060218.t1 MTR_2g015850 51.557 289 132 5 55 339 78 362 1.13e-91 278
MS.gene060218.t1 MTR_2g016270 42.982 342 168 9 9 340 16 340 2.33e-87 271
MS.gene060218.t1 MTR_2g016180 42.651 347 177 9 9 340 16 355 2.48e-87 271
MS.gene060218.t1 MTR_2g067900 47.152 316 145 10 38 338 15 323 4.76e-84 258
MS.gene060218.t1 MTR_2g064475 41.667 348 147 14 38 340 15 351 3.29e-75 236
MS.gene060218.t1 MTR_2g067560 45.031 322 141 14 38 335 15 324 4.18e-73 243
MS.gene060218.t1 MTR_2g015920 38.483 356 180 13 4 332 7 350 1.32e-72 229
MS.gene060218.t1 MTR_7g078630 45.878 279 112 6 33 300 700 950 8.21e-70 234
MS.gene060218.t1 MTR_4g065077 36.538 364 198 10 1 340 2 356 9.53e-69 219
MS.gene060218.t1 MTR_2g066980 35.876 354 165 11 10 340 4 318 2.07e-68 217
MS.gene060218.t1 MTR_4g125300 42.244 303 157 9 45 340 57 348 1.49e-67 216
MS.gene060218.t1 MTR_2g015930 37.396 361 184 15 1 332 3 350 3.23e-66 213
MS.gene060218.t1 MTR_4g071060 36.620 355 189 9 7 332 1 348 3.88e-65 210
MS.gene060218.t1 MTR_8g086470 34.903 361 192 10 1 340 18 356 4.40e-62 202
MS.gene060218.t1 MTR_1g023210 39.088 307 151 10 48 332 60 352 1.30e-61 201
MS.gene060218.t1 MTR_1g023810 44.397 232 120 6 110 338 36 261 4.16e-61 196
MS.gene060218.t1 MTR_1g094135 35.647 317 171 10 37 332 54 358 5.93e-52 176
MS.gene060218.t1 MTR_4g072790 36.364 297 142 11 45 332 54 312 1.57e-46 160
MS.gene060218.t1 MTR_4g072340 35.880 301 142 11 45 332 54 316 5.07e-45 157
MS.gene060218.t1 MTR_4g073660 35.880 301 142 11 45 332 53 315 7.09e-45 156
MS.gene060218.t1 MTR_2g015840 46.835 158 73 3 8 158 3 156 1.94e-43 151
MS.gene060218.t1 MTR_2g015840 54.464 112 50 1 229 340 161 271 8.91e-36 131
MS.gene060218.t1 MTR_7g078580 63.265 98 35 1 242 339 4 100 3.72e-42 143
MS.gene060218.t1 MTR_7g079130 63.736 91 33 0 249 339 6 96 7.80e-37 128
MS.gene060218.t1 MTR_1g025450 38.624 189 89 7 110 293 42 208 5.38e-33 129
MS.gene060218.t1 MTR_7g078310 43.571 140 72 3 79 216 113 247 1.24e-30 117
MS.gene060218.t1 MTR_2g016380 43.590 117 61 3 227 340 21 135 4.55e-27 107
MS.gene060218.t1 MTR_3g029560 29.108 213 121 6 134 340 49 237 5.10e-23 96.3
MS.gene060218.t1 MTR_8g087510 43.511 131 67 5 164 292 23 148 4.87e-21 89.0
MS.gene060218.t1 MTR_7g111060 29.515 227 128 7 129 332 113 330 9.30e-21 92.0
MS.gene060218.t1 MTR_7g111050 30.508 236 136 8 118 332 99 327 3.88e-20 90.5
MS.gene060218.t1 MTR_7g079260 51.389 72 19 1 266 337 7 62 8.91e-19 80.9
MS.gene060218.t1 MTR_2g047960 47.059 85 44 1 132 216 7 90 7.81e-17 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060218.t1 AT5G45890 53.529 340 143 7 14 340 8 345 4.50e-129 373
MS.gene060218.t1 AT5G50260 52.647 340 144 5 14 339 5 341 5.92e-123 358
MS.gene060218.t1 AT3G48340 50.867 346 157 6 3 339 1 342 7.24e-120 350
MS.gene060218.t1 AT2G34080 49.560 341 160 5 10 341 8 345 2.26e-117 343
MS.gene060218.t1 AT3G19390 49.563 343 167 5 2 340 5 345 3.59e-117 346
MS.gene060218.t1 AT4G35350 51.335 337 150 6 13 340 21 352 3.97e-117 343
MS.gene060218.t1 AT1G06260 51.917 339 147 8 10 339 10 341 6.56e-116 339
MS.gene060218.t1 AT3G48350 50.867 346 157 8 5 340 1 343 8.33e-116 340
MS.gene060218.t1 AT2G27420 50.145 345 158 4 10 341 5 348 9.63e-116 339
MS.gene060218.t1 AT3G49340 48.844 346 155 5 11 341 3 341 5.49e-115 337
MS.gene060218.t1 AT5G43060 50.284 352 154 11 1 339 9 352 6.85e-115 341
MS.gene060218.t1 AT1G29090 48.305 354 164 8 3 341 6 355 4.74e-112 330
MS.gene060218.t1 AT3G19400 51.307 306 144 4 39 340 44 348 8.82e-112 329
MS.gene060218.t1 AT1G47128 52.273 308 134 8 39 339 50 351 6.53e-111 331
MS.gene060218.t1 AT1G20850 51.757 313 144 4 32 340 44 353 1.03e-109 324
MS.gene060218.t1 AT4G36880 53.333 315 126 7 41 339 51 360 1.42e-109 324
MS.gene060218.t1 AT1G29080 47.875 353 156 10 10 341 1 346 1.82e-108 320
MS.gene060218.t1 AT1G09850 50.323 310 148 4 33 339 26 332 9.05e-105 314
MS.gene060218.t1 AT4G11310 50.485 309 137 7 40 339 50 351 1.20e-104 311
MS.gene060218.t1 AT4G23520 46.132 349 171 10 1 339 7 348 2.72e-102 305
MS.gene060218.t1 AT4G11320 48.693 306 147 5 40 339 57 358 3.77e-102 305
MS.gene060218.t1 AT4G11320 48.693 306 147 5 40 339 57 358 3.77e-102 305
MS.gene060218.t1 AT3G43960 44.578 332 170 8 16 339 20 345 3.13e-92 280
MS.gene060218.t1 AT1G29110 42.274 343 168 8 13 340 6 333 2.37e-87 266
MS.gene060218.t1 AT4G35350 49.446 271 123 6 13 274 21 286 2.95e-84 256
MS.gene060218.t1 AT3G48350 48.214 280 132 8 5 274 1 277 1.75e-78 242
MS.gene060218.t1 AT3G19400 48.750 240 119 3 39 274 44 283 9.67e-77 238
MS.gene060218.t1 AT3G45310 43.046 302 156 10 45 340 65 356 5.60e-70 223
MS.gene060218.t1 AT5G60360 43.046 302 156 10 45 340 65 356 1.51e-69 221
MS.gene060218.t1 AT3G45310 42.715 302 156 11 45 340 65 355 2.00e-67 216
MS.gene060218.t1 AT5G60360 42.715 302 156 11 45 340 65 355 4.88e-67 215
MS.gene060218.t1 AT5G60360 41.946 298 161 9 45 336 65 356 7.45e-67 214
MS.gene060218.t1 AT4G39090 39.869 306 156 9 45 332 57 352 9.06e-63 204
MS.gene060218.t1 AT2G21430 39.216 306 158 8 45 332 54 349 1.54e-62 203
MS.gene060218.t1 AT4G16190 39.088 307 159 8 45 332 61 358 1.03e-60 199
MS.gene060218.t1 AT3G54940 36.859 312 165 9 42 332 54 354 3.34e-54 182
MS.gene060218.t1 AT3G54940 36.000 225 132 4 42 256 54 276 9.43e-38 136
MS.gene060218.t1 AT1G02305 27.841 352 195 13 11 332 11 333 2.90e-25 104
MS.gene060218.t1 AT4G01610 27.797 295 177 10 70 336 48 334 8.22e-23 98.2
MS.gene060218.t1 AT4G01610 27.458 295 178 10 70 336 48 334 1.48e-21 94.4
MS.gene060218.t1 AT1G02300 30.000 210 123 7 142 332 146 350 1.59e-18 85.9
MS.gene060218.t1 AT2G22160 40.659 91 51 2 54 142 9 98 4.74e-15 70.9

Find 79 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATAAATCTTATAAATT+AGG 0.157228 2.3:-21032608 MS.gene060218:CDS
TCTCATACATAGGGTCATTT+TGG 0.232576 2.3:+21032747 None:intergenic
ATTTGCATTGTTTCTTTGCT+TGG 0.237282 2.3:-21032804 MS.gene060218:CDS
TACAAAAGTGGTGTCTTTAC+TGG 0.255221 2.3:-21031939 MS.gene060218:CDS
AGCTCTGCTGCTACCATTTC+CGG 0.255468 2.3:-21032065 MS.gene060218:CDS
TAAGTTGAGTACAGGAAATT+TGG 0.276268 2.3:-21032264 MS.gene060218:CDS
TTTGCATTGTTTCTTTGCTT+GGG 0.324853 2.3:-21032803 MS.gene060218:CDS
CGTAGCAAATCAACCGGTTT+CGG 0.341741 2.3:-21031997 MS.gene060218:CDS
ACAACATGGCAAAGTCTATA+AGG 0.357194 2.3:-21032705 MS.gene060218:CDS
GACATTCTCGTACCCGGAAA+TGG 0.380302 2.3:+21032052 None:intergenic
GGTGTAGCTGTTGTGGGTTA+TGG 0.386579 2.3:-21031891 MS.gene060218:CDS
AATTATGCAGTTGGATAAGA+TGG 0.402093 2.3:+21031738 None:intergenic
ATTGAATCTTTCAATAATGT+TGG 0.404766 2.3:-21032629 MS.gene060218:CDS
AATATTTGTAGGATGTTGTT+GGG 0.406693 2.3:-21032321 MS.gene060218:intron
ATTGGTTGGTGAAGAATTCA+TGG 0.413527 2.3:-21031845 MS.gene060218:CDS
TACCCTTATCAAGGTGTTGA+TGG 0.417342 2.3:-21032113 MS.gene060218:CDS
GCATCAATGGCTACCGAAAC+CGG 0.418768 2.3:+21031984 None:intergenic
TAATATTTGTAGGATGTTGT+TGG 0.420839 2.3:-21032322 MS.gene060218:intron
GAATTCATGGGGAACTCAAT+GGG 0.434741 2.3:-21031832 MS.gene060218:CDS
GGGGTGAAGAAGGGTACATT+AGG 0.434781 2.3:-21031812 MS.gene060218:CDS
AGGTTGTGAAGGTGGCCTTA+TGG 0.437220 2.3:-21032189 MS.gene060218:CDS
GCTCTGCTGCTACCATTTCC+GGG 0.441657 2.3:-21032064 MS.gene060218:CDS
TTCCATCAACACCTTGATAA+GGG 0.450919 2.3:+21032111 None:intergenic
GTCAAAGCCACTAGCATCAA+TGG 0.451644 2.3:+21031971 None:intergenic
GCTGTTGTGGGTTATGGTGT+TGG 0.453735 2.3:-21031885 MS.gene060218:CDS
GATCATGGTGTAGCTGTTGT+GGG 0.460675 2.3:-21031897 MS.gene060218:CDS
GGAACTCAATGGGGTGAAGA+AGG 0.462980 2.3:-21031822 MS.gene060218:CDS
GTTCCATCAACACCTTGATA+AGG 0.464383 2.3:+21032110 None:intergenic
TGCTCATGCCTCTCATACAT+AGG 0.466056 2.3:+21032737 None:intergenic
AAATCTTATAATTATGCAGT+TGG 0.468251 2.3:+21031729 None:intergenic
GCAACTAGAAACAAATTCAA+TGG 0.478900 2.3:-21032545 MS.gene060218:CDS
GGATCATGGTGTAGCTGTTG+TGG 0.482554 2.3:-21031898 MS.gene060218:CDS
ACTCAACTTATGAATTCCTT+CGG 0.492619 2.3:+21032277 None:intergenic
CATTAGGATGCAAAGGGGTG+TGG 0.492848 2.3:-21031796 MS.gene060218:CDS
AGAATTCATGGGGAACTCAA+TGG 0.494609 2.3:-21031833 MS.gene060218:CDS
ATTCGAAAGCATCATCCATA+AGG 0.507986 2.3:+21032174 None:intergenic
TACCATCTGCAGTGGATTGG+AGG 0.508009 2.3:-21032469 MS.gene060218:CDS
CGAAACCGGTTGATTTGCTA+CGG 0.509364 2.3:+21031998 None:intergenic
GGAATTCATAAGTTGAGTAC+AGG 0.513328 2.3:-21032272 MS.gene060218:CDS
TTCGGGGACATTCTCGTACC+CGG 0.515155 2.3:+21032046 None:intergenic
ACATGCAATAAAACTGAAGT+AGG 0.516912 2.3:-21032089 MS.gene060218:CDS
ATCTGCAGTGGATTGGAGGC+AGG 0.519791 2.3:-21032465 MS.gene060218:CDS
AGGGTACATTAGGATGCAAA+GGG 0.524704 2.3:-21031802 MS.gene060218:CDS
AAAAGCCGTAGCAAATCAAC+CGG 0.525968 2.3:-21032003 MS.gene060218:CDS
TGTCCCCGAAAATGATGAGC+AGG 0.531895 2.3:-21032033 MS.gene060218:CDS
TTCTAGTTGCTACAAACTCA+TGG 0.534672 2.3:+21032558 None:intergenic
TCGGTAGCCATTGATGCTAG+TGG 0.545275 2.3:-21031978 MS.gene060218:CDS
AAATTCAATGGATATTTGCA+TGG 0.546885 2.3:-21032533 MS.gene060218:CDS
AAGGGTACATTAGGATGCAA+AGG 0.551462 2.3:-21031803 MS.gene060218:CDS
TAATGGACTTAGCACTGAAG+TGG 0.556033 2.3:-21032138 MS.gene060218:CDS
TTGGTTGGTGAAGAATTCAT+GGG 0.557529 2.3:-21031844 MS.gene060218:CDS
TCGGCTGTTGCATCAACCGA+AGG 0.561296 2.3:-21032293 MS.gene060218:CDS
TGTGGAACTGAGTTGGATCA+TGG 0.564256 2.3:-21031912 MS.gene060218:CDS
GAACTCAATGGGGTGAAGAA+GGG 0.565034 2.3:-21031821 MS.gene060218:CDS
GAAGTGGAATACCCTTATCA+AGG 0.568429 2.3:-21032122 MS.gene060218:CDS
GCTCATGCCTCTCATACATA+GGG 0.569140 2.3:+21032738 None:intergenic
GTGAAGATGAGACTGAGTAT+TGG 0.569553 2.3:-21031863 MS.gene060218:CDS
ATGTACCATCTGCAGTGGAT+TGG 0.570020 2.3:-21032472 MS.gene060218:CDS
TGCCTCCAATCCACTGCAGA+TGG 0.579586 2.3:+21032467 None:intergenic
TAAGGCCACCTTCACAACCT+TGG 0.583050 2.3:+21032192 None:intergenic
TCTATAAGGATGCACATGAA+AGG 0.584380 2.3:-21032691 MS.gene060218:CDS
TGGTTCATGTGGAACTGAGT+TGG 0.588339 2.3:-21031919 MS.gene060218:CDS
AGGGGTGTGGATGCTTCAGA+AGG 0.605030 2.3:-21031783 MS.gene060218:CDS
TGTATGAGAGGCATGAGCAA+TGG 0.614507 2.3:-21032733 MS.gene060218:CDS
GCAGTGGATTGGAGGCAGGA+AGG 0.619784 2.3:-21032461 MS.gene060218:CDS
AGATGAGACTGAGTATTGGT+TGG 0.624949 2.3:-21031859 MS.gene060218:CDS
GAAGACCAAGGTTGTGAAGG+TGG 0.628652 2.3:-21032197 MS.gene060218:CDS
AAAATGACCCTATGTATGAG+AGG 0.630864 2.3:-21032745 MS.gene060218:CDS
GGTGTCTTTACTGGTTCATG+TGG 0.637602 2.3:-21031930 MS.gene060218:CDS
GGTGAAGACCAAGGTTGTGA+AGG 0.642150 2.3:-21032200 MS.gene060218:CDS
GATGCTTCAGAAGGAATATG+TGG 0.650231 2.3:-21031774 MS.gene060218:CDS
CTTGTTGATTGTGACACAAG+TGG 0.664457 2.3:-21032221 MS.gene060218:CDS
AATTCATGGGGAACTCAATG+GGG 0.670782 2.3:-21031831 MS.gene060218:CDS
TGAGAGGCATGAGCAATGGA+TGG 0.686001 2.3:-21032729 MS.gene060218:CDS
GAGTGATGTACCATCTGCAG+TGG 0.686438 2.3:-21032477 MS.gene060218:CDS
TGGTTGGTGAAGAATTCATG+GGG 0.692894 2.3:-21031843 MS.gene060218:CDS
GAGCAATGGATGGTACAACA+TGG 0.707448 2.3:-21032719 MS.gene060218:CDS
GACACAAGTGGTGAAGACCA+AGG 0.761833 2.3:-21032209 MS.gene060218:CDS
GGGTACATTAGGATGCAAAG+GGG 0.770853 2.3:-21031801 MS.gene060218:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTTATATAACAATGA+AGG - chr2.3:21032183-21032202 MS.gene060218:CDS 10.0%
!! GAATTCATTATAAAAAATAA+TGG - chr2.3:21032421-21032440 MS.gene060218:intron 10.0%
!! GGAAATAAATCTTATAAATT+AGG - chr2.3:21031968-21031987 MS.gene060218:CDS 15.0%
!!! AACTAAATTTTACTATTACA+AGG + chr2.3:21032228-21032247 None:intergenic 15.0%
!! AGTTGTGATTTAATATTTGT+AGG - chr2.3:21032244-21032263 MS.gene060218:CDS 20.0%
!! ATTGAATCTTTCAATAATGT+TGG - chr2.3:21031947-21031966 MS.gene060218:CDS 20.0%
! AAATTCAATGGATATTTGCA+TGG - chr2.3:21032043-21032062 MS.gene060218:CDS 25.0%
! AATATTTGTAGGATGTTGTT+GGG - chr2.3:21032255-21032274 MS.gene060218:CDS 25.0%
! TAATATTTGTAGGATGTTGT+TGG - chr2.3:21032254-21032273 MS.gene060218:CDS 25.0%
! TTTATATAACAATGAAGGTG+TGG - chr2.3:21032188-21032207 MS.gene060218:CDS 25.0%
!! GACTTTCAATTTTACAAAAG+TGG - chr2.3:21032625-21032644 MS.gene060218:CDS 25.0%
ACATGCAATAAAACTGAAGT+AGG - chr2.3:21032487-21032506 MS.gene060218:CDS 30.0%
ACTCAACTTATGAATTCCTT+CGG + chr2.3:21032302-21032321 None:intergenic 30.0%
GCAACTAGAAACAAATTCAA+TGG - chr2.3:21032031-21032050 MS.gene060218:CDS 30.0%
! ATGAAAGGCAAAAACGTTTT+GGG - chr2.3:21031900-21031919 MS.gene060218:CDS 30.0%
! ATTTGCATTGTTTCTTTGCT+TGG - chr2.3:21031772-21031791 MS.gene060218:CDS 30.0%
! TAAGTTGAGTACAGGAAATT+TGG - chr2.3:21032312-21032331 MS.gene060218:CDS 30.0%
! TTTGCATTGTTTCTTTGCTT+GGG - chr2.3:21031773-21031792 MS.gene060218:CDS 30.0%
AAAATGACCCTATGTATGAG+AGG - chr2.3:21031831-21031850 MS.gene060218:CDS 35.0%
ACAACATGGCAAAGTCTATA+AGG - chr2.3:21031871-21031890 MS.gene060218:CDS 35.0%
GTGAAAAATCAAGGTCAATG+TGG - chr2.3:21032151-21032170 MS.gene060218:CDS 35.0%
TACAAAAGTGGTGTCTTTAC+TGG - chr2.3:21032637-21032656 MS.gene060218:CDS 35.0%
TCTATAAGGATGCACATGAA+AGG - chr2.3:21031885-21031904 MS.gene060218:CDS 35.0%
TTCCATCAACACCTTGATAA+GGG + chr2.3:21032468-21032487 None:intergenic 35.0%
! CATGAAAGGCAAAAACGTTT+TGG - chr2.3:21031899-21031918 MS.gene060218:CDS 35.0%
! CATTGACCTTGATTTTTCAC+AGG + chr2.3:21032151-21032170 None:intergenic 35.0%
! GGAATTCATAAGTTGAGTAC+AGG - chr2.3:21032304-21032323 MS.gene060218:CDS 35.0%
! TCTCATACATAGGGTCATTT+TGG + chr2.3:21031832-21031851 None:intergenic 35.0%
! TTCTAGTTGCTACAAACTCA+TGG + chr2.3:21032021-21032040 None:intergenic 35.0%
!! ATTCGAAAGCATCATCCATA+AGG + chr2.3:21032405-21032424 None:intergenic 35.0%
!! ATTGGTTGGTGAAGAATTCA+TGG - chr2.3:21032731-21032750 MS.gene060218:CDS 35.0%
!! TTGGTTGGTGAAGAATTCAT+GGG - chr2.3:21032732-21032751 MS.gene060218:CDS 35.0%
AAAAGCCGTAGCAAATCAAC+CGG - chr2.3:21032573-21032592 MS.gene060218:CDS 40.0%
AATTCATGGGGAACTCAATG+GGG - chr2.3:21032745-21032764 MS.gene060218:CDS 40.0%
AGAATTCATGGGGAACTCAA+TGG - chr2.3:21032743-21032762 MS.gene060218:CDS 40.0%
AGGGTACATTAGGATGCAAA+GGG - chr2.3:21032774-21032793 MS.gene060218:CDS 40.0%
GAAGTGGAATACCCTTATCA+AGG - chr2.3:21032454-21032473 MS.gene060218:CDS 40.0%
GAATTCATGGGGAACTCAAT+GGG - chr2.3:21032744-21032763 MS.gene060218:CDS 40.0%
GATGCTTCAGAAGGAATATG+TGG - chr2.3:21032802-21032821 MS.gene060218:CDS 40.0%
GTCACACCTGTGAAAAATCA+AGG - chr2.3:21032142-21032161 MS.gene060218:CDS 40.0%
GTGAAGATGAGACTGAGTAT+TGG - chr2.3:21032713-21032732 MS.gene060218:CDS 40.0%
GTTCCATCAACACCTTGATA+AGG + chr2.3:21032469-21032488 None:intergenic 40.0%
! AAGGGTACATTAGGATGCAA+AGG - chr2.3:21032773-21032792 MS.gene060218:CDS 40.0%
! AATGCCTGCTCATCATTTTC+GGG + chr2.3:21032550-21032569 None:intergenic 40.0%
! CAATGCCTGCTCATCATTTT+CGG + chr2.3:21032551-21032570 None:intergenic 40.0%
! CTTGTTGATTGTGACACAAG+TGG - chr2.3:21032355-21032374 MS.gene060218:intron 40.0%
!! AGATGAGACTGAGTATTGGT+TGG - chr2.3:21032717-21032736 MS.gene060218:CDS 40.0%
!! TAATGGACTTAGCACTGAAG+TGG - chr2.3:21032438-21032457 MS.gene060218:CDS 40.0%
!! TACCCTTATCAAGGTGTTGA+TGG - chr2.3:21032463-21032482 MS.gene060218:CDS 40.0%
!! TGGTTGGTGAAGAATTCATG+GGG - chr2.3:21032733-21032752 MS.gene060218:CDS 40.0%
ATGTACCATCTGCAGTGGAT+TGG - chr2.3:21032104-21032123 MS.gene060218:CDS 45.0%
GAACTCAATGGGGTGAAGAA+GGG - chr2.3:21032755-21032774 MS.gene060218:CDS 45.0%
GAGCAATGGATGGTACAACA+TGG - chr2.3:21031857-21031876 MS.gene060218:CDS 45.0%
GATCATGGTGTAGCTGTTGT+GGG - chr2.3:21032679-21032698 MS.gene060218:CDS 45.0%
GCTCATGCCTCTCATACATA+GGG + chr2.3:21031841-21031860 None:intergenic 45.0%
GGGTACATTAGGATGCAAAG+GGG - chr2.3:21032775-21032794 MS.gene060218:CDS 45.0%
GTCAAAGCCACTAGCATCAA+TGG + chr2.3:21032608-21032627 None:intergenic 45.0%
TGCTCATGCCTCTCATACAT+AGG + chr2.3:21031842-21031861 None:intergenic 45.0%
TGGTTCATGTGGAACTGAGT+TGG - chr2.3:21032657-21032676 MS.gene060218:CDS 45.0%
! AGGATGTTGTTGGGCGTTTT+CGG - chr2.3:21032264-21032283 MS.gene060218:CDS 45.0%
! ATGCCTGCTCATCATTTTCG+GGG + chr2.3:21032549-21032568 None:intergenic 45.0%
! CGTAGCAAATCAACCGGTTT+CGG - chr2.3:21032579-21032598 MS.gene060218:CDS 45.0%
! TGTATGAGAGGCATGAGCAA+TGG - chr2.3:21031843-21031862 MS.gene060218:CDS 45.0%
! TGTGGAACTGAGTTGGATCA+TGG - chr2.3:21032664-21032683 MS.gene060218:CDS 45.0%
!! CGAAACCGGTTGATTTGCTA+CGG + chr2.3:21032581-21032600 None:intergenic 45.0%
!! GGTGTCTTTACTGGTTCATG+TGG - chr2.3:21032646-21032665 MS.gene060218:CDS 45.0%
AGCTCTGCTGCTACCATTTC+CGG - chr2.3:21032511-21032530 MS.gene060218:CDS 50.0%
CATTAGGATGCAAAGGGGTG+TGG - chr2.3:21032780-21032799 MS.gene060218:CDS 50.0%
GAAGACCAAGGTTGTGAAGG+TGG - chr2.3:21032379-21032398 MS.gene060218:intron 50.0%
GACACAAGTGGTGAAGACCA+AGG - chr2.3:21032367-21032386 MS.gene060218:intron 50.0%
GACATTCTCGTACCCGGAAA+TGG + chr2.3:21032527-21032546 None:intergenic 50.0%
GAGTGATGTACCATCTGCAG+TGG - chr2.3:21032099-21032118 MS.gene060218:CDS 50.0%
GCATCAATGGCTACCGAAAC+CGG + chr2.3:21032595-21032614 None:intergenic 50.0%
GGAACTCAATGGGGTGAAGA+AGG - chr2.3:21032754-21032773 MS.gene060218:CDS 50.0%
GGATCATGGTGTAGCTGTTG+TGG - chr2.3:21032678-21032697 MS.gene060218:CDS 50.0%
GGTGAAGACCAAGGTTGTGA+AGG - chr2.3:21032376-21032395 MS.gene060218:intron 50.0%
TAAGGCCACCTTCACAACCT+TGG + chr2.3:21032387-21032406 None:intergenic 50.0%
TCGGTAGCCATTGATGCTAG+TGG - chr2.3:21032598-21032617 MS.gene060218:CDS 50.0%
TGTCCCCGAAAATGATGAGC+AGG - chr2.3:21032543-21032562 MS.gene060218:CDS 50.0%
! AGGTTGTGAAGGTGGCCTTA+TGG - chr2.3:21032387-21032406 MS.gene060218:intron 50.0%
! GCTGTTGTGGGTTATGGTGT+TGG - chr2.3:21032691-21032710 MS.gene060218:CDS 50.0%
! GGGGTGAAGAAGGGTACATT+AGG - chr2.3:21032764-21032783 MS.gene060218:CDS 50.0%
! GGTGTAGCTGTTGTGGGTTA+TGG - chr2.3:21032685-21032704 MS.gene060218:CDS 50.0%
! TGAGAGGCATGAGCAATGGA+TGG - chr2.3:21031847-21031866 MS.gene060218:CDS 50.0%
!! TACCATCTGCAGTGGATTGG+AGG - chr2.3:21032107-21032126 MS.gene060218:CDS 50.0%
GCTCTGCTGCTACCATTTCC+GGG - chr2.3:21032512-21032531 MS.gene060218:CDS 55.0%
TCGGCTGTTGCATCAACCGA+AGG - chr2.3:21032283-21032302 MS.gene060218:CDS 55.0%
TGCCTCCAATCCACTGCAGA+TGG + chr2.3:21032112-21032131 None:intergenic 55.0%
TTCGGGGACATTCTCGTACC+CGG + chr2.3:21032533-21032552 None:intergenic 55.0%
! AGGGGTGTGGATGCTTCAGA+AGG - chr2.3:21032793-21032812 MS.gene060218:CDS 55.0%
!! ATCTGCAGTGGATTGGAGGC+AGG - chr2.3:21032111-21032130 MS.gene060218:CDS 55.0%
!! GCAGTGGATTGGAGGCAGGA+AGG - chr2.3:21032115-21032134 MS.gene060218:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 21031740 21032858 21031740 ID=MS.gene060218
chr2.3 mRNA 21031740 21032858 21031740 ID=MS.gene060218.t1;Parent=MS.gene060218
chr2.3 exon 21032426 21032858 21032426 ID=MS.gene060218.t1.exon1;Parent=MS.gene060218.t1
chr2.3 CDS 21032426 21032858 21032426 ID=cds.MS.gene060218.t1;Parent=MS.gene060218.t1
chr2.3 exon 21031740 21032332 21031740 ID=MS.gene060218.t1.exon2;Parent=MS.gene060218.t1
chr2.3 CDS 21031740 21032332 21031740 ID=cds.MS.gene060218.t1;Parent=MS.gene060218.t1
Gene Sequence

>MS.gene060218

ATGGTTATGAATATTCAATTACATTATATTTCATTTGCATTGTTTCTTTGCTTGGGATTGTTGAGTTTTCAAGCTACATCTCGAACTCTCCAAAATGACCCTATGTATGAGAGGCATGAGCAATGGATGGTACAACATGGCAAAGTCTATAAGGATGCACATGAAAGGCAAAAACGTTTTGGGATATTCAAAGAAAATGTGAATTACATTGAATCTTTCAATAATGTTGGAAATAAATCTTATAAATTAGGACTTAATCATTTTGCTGATCTTAGTAACCATGAGTTTGTAGCAACTAGAAACAAATTCAATGGATATTTGCATGGCTCAAGCATAACTACTTTTAAGTACAAAAATGTGAGTGATGTACCATCTGCAGTGGATTGGAGGCAGGAAGGAGCAGTCACACCTGTGAAAAATCAAGGTCAATGTGGTAAGTGTTTATTTATTTATATAACAATGAAGGTGTGGTTTCTTATTTTTAACCTTGTAATAGTAAAATTTAGTTGTGATTTAATATTTGTAGGATGTTGTTGGGCGTTTTCGGCTGTTGCATCAACCGAAGGAATTCATAAGTTGAGTACAGGAAATTTGGTCTCTTTATCAGAACAAGAACTTGTTGATTGTGACACAAGTGGTGAAGACCAAGGTTGTGAAGGTGGCCTTATGGATGATGCTTTCGAATTCATTATAAAAAATAATGGACTTAGCACTGAAGTGGAATACCCTTATCAAGGTGTTGATGGAACATGCAATAAAACTGAAGTAGGCAGCTCTGCTGCTACCATTTCCGGGTACGAGAATGTCCCCGAAAATGATGAGCAGGCATTGCAAAAAGCCGTAGCAAATCAACCGGTTTCGGTAGCCATTGATGCTAGTGGCTTTGACTTTCAATTTTACAAAAGTGGTGTCTTTACTGGTTCATGTGGAACTGAGTTGGATCATGGTGTAGCTGTTGTGGGTTATGGTGTTGGTGAAGATGAGACTGAGTATTGGTTGGTGAAGAATTCATGGGGAACTCAATGGGGTGAAGAAGGGTACATTAGGATGCAAAGGGGTGTGGATGCTTCAGAAGGAATATGTGGTATTGCAATGCAACCATCTTATCCAACTGCATAA
Protein sequence

>MS.gene060218.t1

MVMNIQLHYISFALFLCLGLLSFQATSRTLQNDPMYERHEQWMVQHGKVYKDAHERQKRFGIFKENVNYIESFNNVGNKSYKLGLNHFADLSNHEFVATRNKFNGYLHGSSITTFKYKNVSDVPSAVDWRQEGAVTPVKNQGQCGCCWAFSAVASTEGIHKLSTGNLVSLSEQELVDCDTSGEDQGCEGGLMDDAFEFIIKNNGLSTEVEYPYQGVDGTCNKTEVGSSAATISGYENVPENDEQALQKAVANQPVSVAIDASGFDFQFYKSGVFTGSCGTELDHGVAVVGYGVGEDETEYWLVKNSWGTQWGEEGYIRMQRGVDASEGICGIAMQPSYPTA